pycom12g01560

fructose-bisphosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001206
Physical Location & Seq
Forward (+)
1277312 .. 1279881
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g01560.1

Sequence Viewer

Length: 1227 bp
ATGTATATGCAACTTTCGAGCCGTACTCGCATAAAAATCCTCACCCACCCTTCTTTTTCTTCCATCATCTCTTCCTCTCTCTCGATCTCTCAGAAAACAACCAATTCGTCCTTTTCTTCCTCTCGCGATCTTCAGCTTCAATCAAACATGTCTGCTTACAGAGGCAAATACGCTGATGAGCTTATCGCCAATGCTGCCTACATCGGCACCCCTGGAAAGGGTATTCTCGCTGCTGATGAGTCAACTGGCACAATTGGCAAGCGTTTTGCCAGCATCAATGTTGAGAATGTTGAATCAAACAGGCGTTGTCTTCGTGAACTCCTCTTCACTGCCCCTGATGTGCTCCAATACCTTAGTGGAGTTATCCTCTTTGAGGAAACCCTCTACCAGAAGACCGCTTCAGGCAAGCCATTCGTTGATGTCTTGAAGGAAGGTGGTGTTCTCCCTGGAATTAAGGTTGACAAGGGTACCGTTGAGCTTGCCGGAACCAATGGTGAGACCACAACCCAGGGTCTTGATGGCCTTGCCCAGCGTTGCCAGAAGTACTATGAAGCTGGTGCACGGTTTGCAAAGTGGCGTGCTGTGCTCAAGATTGGCCCCACCGAACCATCTCAGCTGTCCATCCAAGAGAATGCTAACGGGTTGGCTCGTTATGCCATCATCTGCCAGGAGAATGGCCTAGTCCCCATCGTTGAGCCAGAGATCGATCTGGTTGATGGACCCCATTCCATCGATAAGTGTGCTGATGTGACCGAACGTGTTCTTGCCGCATGCTACAAGGCCCTTAACGACCACCATGTCCTGCTTGAAGGAACTCTCTTGAAACCCAATATGGTTACCCCAGGATCTGATGCCAAGAAGGCTTCACCAAAAGTGATTGCTGAGTACACAGTTCGTGCCTTGTTGCGCACTACCCCTGCAGCAGTCCCTGCTGTTGTGTTCTTATCCGGTGGACAGAGTGAGGAGGAGGCTACCCTGAACCTCAACGCCATGAACCAACTCAAGGGAAAGAAGCCGTGGTCTCTATCCTTCTCTTTCGGACGTGCTCTTCAGCAGAGCACACTGAAGGCATGGGCAGGGAAAGAGGAAAACATCCCAAAGGCGCAGGCTGCTCTCCTCACAAGGGCCAAGGCCAACTCAGAAGCTACTCTTGGAATCTACAAGGGTGATGCCAAGCTCGGTGAGGGTGCCGCTGAGTCTCTCCATGTGAAGGATTACAAGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00680 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00030 map00051 map00680 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
Glycolytic

Protein Analysis

409

Amino Acids

44.05

Weight (kDa)

8.73

Isoelectric Point (pI)

33.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycolytic PF00274 60 - 408 4e-170 Fructose-bisphosphate aldolase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 797
Acc16I TGCGCA 1 cut(s) 908
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 3 cut(s) 206, 467, 1187
AccII CGCG 1 cut(s) 126
AciI CCGC 3 cut(s) 396, 768, 1191
AclWI GGATC 1 cut(s) 853
AcuI CTGAAG 4 cut(s) 116, 384, 1034, 1085
AfaI GTAC 5 cut(s) 25, 469, 545, 887, 1223
AfiI CCNNNNNNNGG 6 cut(s) 217, 218, 373, 1003, 1123, 1210
AflIII ACRYGT 2 cut(s) 147, 757
AgsI TTSAA 5 cut(s) 140, 293, 427, 809, 823
AjiI CACGTC 1 cut(s) 1043
AjnI CCWGG 5 cut(s) 211, 445, 507, 666, 841
AjuI GAANNNNNNNTTGG 2 cut(s) 1134, 1166
AluBI AGCT 7 cut(s) 136, 181, 478, 554, 616, 1145, 1177
AluI AGCT 7 cut(s) 136, 181, 478, 554, 616, 1145, 1177
Alw21I GWGCWC 5 cut(s) 345, 562, 588, 1048, 1061
Alw26I GTCTC 3 cut(s) 491, 1026, 1203
Alw44I GTGCAC 1 cut(s) 558
AlwI GGATC 1 cut(s) 853
AlwNI CAGNNNCTG 2 cut(s) 848, 929
AoxI GGCC 6 cut(s) 520, 595, 676, 780, 1125, 1131
ApaLI GTGCAC 1 cut(s) 558
ApeKI GCWGC 4 cut(s) 194, 230, 920, 1109
Asp700I GAANNNNTTC 1 cut(s) 759
Asp718I GGTACC 1 cut(s) 467
AspLEI GCGC 2 cut(s) 909, 1105
AspS9I GGNCC 4 cut(s) 596, 719, 781, 1125
AsuHPI GGTGA 5 cut(s) 34, 506, 858, 1178, 1193
AvaII GGWCC 1 cut(s) 719
BaeGI GKGCMC 1 cut(s) 562
BaeI ACNNNNGTAYC 2 cut(s) 451, 484
BanI GGYRCC 3 cut(s) 206, 467, 1187
BbsI GAAGAC 2 cut(s) 302, 398
Bbv12I GWGCWC 5 cut(s) 345, 562, 588, 1048, 1061
BbvI GCAGC 4 cut(s) 181, 217, 932, 1096
BccI CCATC 8 cut(s) 71, 512, 616, 629, 665, 695, 710, 737
BceAI ACGGC 2 cut(s) 6, 1000
BcgI CGANNNNNNTGC 4 cut(s) 394, 428, 722, 756
BciT130I CCWGG 5 cut(s) 213, 447, 509, 668, 843
BcoDI GTCTC 3 cut(s) 491, 1026, 1203
BfaI CTAG 1 cut(s) 680
BfmI CTRYAG 1 cut(s) 918
BglI GCCNNNNNGGC 1 cut(s) 860
BisI GCNGC 6 cut(s) 195, 231, 768, 921, 1110, 1191
BlsI GCNGC 6 cut(s) 196, 232, 769, 922, 1111, 1192
BmcAI AGTACT 2 cut(s) 545, 1223
Bme1390I CCNGG 5 cut(s) 213, 447, 509, 668, 843
Bme18I GGWCC 1 cut(s) 719
BmgBI CACGTC 1 cut(s) 1043
BmgT120I GGNCC 4 cut(s) 596, 719, 781, 1125
BmiI GGNNCC 6 cut(s) 208, 469, 487, 598, 721, 1189
BmrFI CCNGG 5 cut(s) 213, 447, 509, 668, 843
BmsI GCATC 3 cut(s) 282, 841, 1159
BpiI GAAGAC 2 cut(s) 302, 398
BplI GAGNNNNNCTC 4 cut(s) 10, 42, 351, 383
BpuEI CTTGAG 2 cut(s) 572, 986
Bsa29I ATCGAT 2 cut(s) 705, 732
BsaI GGTCTC 2 cut(s) 491, 1026
BsaJI CCNNGG 7 cut(s) 211, 445, 507, 508, 841, 1016, 1128
BsaWI WCCGGW 1 cut(s) 947
Bsc4I CCNNNNNNNGG 6 cut(s) 217, 218, 373, 1003, 1123, 1210
Bse1I ACTGG 1 cut(s) 250
BseBI CCWGG 5 cut(s) 213, 447, 509, 668, 843
BseCI ATCGAT 2 cut(s) 705, 732
BseDI CCNNGG 7 cut(s) 211, 445, 507, 508, 841, 1016, 1128
BseGI GGATG 2 cut(s) 621, 1092
BseLI CCNNNNNNNGG 6 cut(s) 217, 218, 373, 1003, 1123, 1210
BseMII CTCAG 5 cut(s) 104, 626, 873, 1152, 1185
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 4 cut(s) 311, 977, 980, 1106
BseSI GKGCMC 1 cut(s) 562
BseXI GCAGC 4 cut(s) 181, 217, 932, 1096
BseYI CCCAGC 1 cut(s) 528
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 6 cut(s) 522, 597, 678, 782, 1127, 1133
BshNI GGYRCC 3 cut(s) 206, 467, 1187
BshVI ATCGAT 2 cut(s) 705, 732
BsiHKAI GWGCWC 5 cut(s) 345, 562, 588, 1048, 1061
BsiSI CCGG 2 cut(s) 483, 948
BslFI GGGAC 2 cut(s) 668, 911
BslI CCNNNNNNNGG 6 cut(s) 217, 218, 373, 1003, 1123, 1210
BsmAI GTCTC 3 cut(s) 491, 1026, 1203
BsmFI GGGAC 2 cut(s) 668, 911
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 6 cut(s) 522, 597, 678, 782, 1127, 1133
Bso31I GGTCTC 2 cut(s) 491, 1026
Bsp1286I GDGCHC 5 cut(s) 345, 562, 588, 1048, 1061
Bsp143I GATC 5 cut(s) 84, 127, 702, 706, 845
Bsp68I TCGCGA 1 cut(s) 126
BspACI CCGC 3 cut(s) 396, 768, 1191
BspANI GGCC 6 cut(s) 522, 597, 678, 782, 1127, 1133
BspCNI CTCAG 5 cut(s) 103, 625, 874, 1151, 1186
BspDI ATCGAT 2 cut(s) 705, 732
BspFNI CGCG 1 cut(s) 126
BspLI GGNNCC 6 cut(s) 208, 469, 487, 598, 721, 1189
BspMAI CTGCAG 1 cut(s) 922
BspPI GGATC 1 cut(s) 853
BspQI GCTCTTC 1 cut(s) 1053
BspT107I GGYRCC 3 cut(s) 206, 467, 1187
BspTNI GGTCTC 2 cut(s) 491, 1026
BsrI ACTGG 1 cut(s) 250
BssECI CCNNGG 7 cut(s) 211, 445, 507, 508, 841, 1016, 1128
BssMI GATC 5 cut(s) 84, 127, 702, 706, 845
BssT1I CCWWGG 1 cut(s) 1128
Bst2UI CCWGG 5 cut(s) 213, 447, 509, 668, 843
Bst4CI ACNGT 3 cut(s) 472, 564, 892
Bst6I CTCTTC 3 cut(s) 76, 329, 1053
BstAPI GCANNNNNTGC 2 cut(s) 566, 929
BstC8I GCNNGC 7 cut(s) 260, 271, 407, 480, 579, 772, 1107
BstDEI CTNAG 6 cut(s) 90, 353, 612, 882, 1138, 1194
BstDSI CCRYGG 1 cut(s) 1016
BstEII GGTNACC 1 cut(s) 835
BstENI CCTNNNNNAGG 1 cut(s) 371
BstF5I GGATG 2 cut(s) 621, 1092
BstFNI CGCG 1 cut(s) 126
BstHHI GCGC 2 cut(s) 909, 1105
BstKTI GATC 5 cut(s) 87, 130, 705, 709, 848
BstMAI GTCTC 3 cut(s) 491, 1026, 1203
BstMBI GATC 5 cut(s) 84, 127, 702, 706, 845
BstMWI GCNNNNNNNGC 9 cut(s) 27, 194, 255, 566, 583, 653, 860, 929, 1109
BstNI CCWGG 5 cut(s) 213, 447, 509, 668, 843
BstNSI RCATGY 2 cut(s) 151, 774
BstPI GGTNACC 1 cut(s) 835
BstSCI CCNGG 5 cut(s) 211, 445, 507, 666, 841
BstSFI CTRYAG 1 cut(s) 918
BstSLI GKGCMC 1 cut(s) 562
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 4 cut(s) 181, 217, 932, 1096
BstV2I GAAGAC 2 cut(s) 302, 398
BstX2I RGATCY 1 cut(s) 845
BstXI CCANNNNNNTGG 1 cut(s) 674
BstYI RGATCY 1 cut(s) 845
Bsu15I ATCGAT 2 cut(s) 705, 732
BsuRI GGCC 6 cut(s) 522, 597, 678, 782, 1127, 1133
BsuTUI ATCGAT 2 cut(s) 705, 732
BtgI CCRYGG 1 cut(s) 1016
BtrI CACGTC 1 cut(s) 1043
BtsCI GGATG 2 cut(s) 621, 1092
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 2 cut(s) 327, 1061
BtuMI TCGCGA 1 cut(s) 126
Cac8I GCNNGC 7 cut(s) 260, 271, 407, 480, 579, 772, 1107
CaiI CAGNNNCTG 2 cut(s) 848, 929
CfoI GCGC 2 cut(s) 909, 1105
Cfr13I GGNCC 4 cut(s) 596, 719, 781, 1125
ClaI ATCGAT 2 cut(s) 705, 732
Csp6I GTAC 5 cut(s) 24, 468, 544, 886, 1222
CviAII CATG 6 cut(s) 148, 771, 797, 991, 1071, 1205
CviQI GTAC 5 cut(s) 24, 468, 544, 886, 1222
DdeI CTNAG 6 cut(s) 90, 353, 612, 882, 1138, 1194
DpnI GATC 5 cut(s) 86, 129, 704, 708, 847
DpnII GATC 5 cut(s) 84, 127, 702, 706, 845
DrdI GACNNNNNNGTC 1 cut(s) 797
DseDI GACNNNNNNGTC 1 cut(s) 797
Eam1104I CTCTTC 3 cut(s) 76, 329, 1053
EarI CTCTTC 3 cut(s) 76, 329, 1053
Eco130I CCWWGG 1 cut(s) 1128
Eco31I GGTCTC 2 cut(s) 491, 1026
Eco47I GGWCC 1 cut(s) 719
Eco57I CTGAAG 4 cut(s) 116, 384, 1034, 1085
Eco91I GGTNACC 1 cut(s) 835
EcoNI CCTNNNNNAGG 1 cut(s) 371
EcoO109I RGGNCCY 1 cut(s) 781
EcoO65I GGTNACC 1 cut(s) 835
EcoRII CCWGG 5 cut(s) 211, 445, 507, 666, 841
EcoT14I CCWWGG 1 cut(s) 1128
ErhI CCWWGG 1 cut(s) 1128
FaeI CATG 6 cut(s) 151, 774, 800, 994, 1074, 1208
FalI AAGNNNNNCTT 2 cut(s) 1134, 1166
FaqI GGGAC 2 cut(s) 668, 911
FatI CATG 6 cut(s) 147, 770, 796, 990, 1070, 1204
Fnu4HI GCNGC 6 cut(s) 195, 231, 768, 921, 1110, 1191
FokI GGATG 2 cut(s) 608, 1079
Fsp4HI GCNGC 6 cut(s) 195, 231, 768, 921, 1110, 1191
FspBI CTAG 1 cut(s) 680
FspI TGCGCA 1 cut(s) 908
GlaI GCGC 2 cut(s) 908, 1104
GluI GCNGC 6 cut(s) 195, 231, 768, 921, 1110, 1191
GsaI CCCAGC 1 cut(s) 532
HaeIII GGCC 6 cut(s) 522, 597, 678, 782, 1127, 1133
HapII CCGG 2 cut(s) 483, 948
HhaI GCGC 2 cut(s) 909, 1105
Hin1II CATG 6 cut(s) 151, 774, 800, 994, 1074, 1208
Hin6I GCGC 2 cut(s) 907, 1103
HinP1I GCGC 2 cut(s) 907, 1103
HincII GTYRAC 2 cut(s) 243, 460
HindII GTYRAC 2 cut(s) 243, 460
HinfI GANTC 4 cut(s) 239, 293, 1155, 1196
HpaII CCGG 2 cut(s) 483, 948
HphI GGTGA 5 cut(s) 34, 506, 858, 1178, 1193
Hpy166II GTNNAC 6 cut(s) 243, 317, 460, 560, 888, 953
Hpy188I TCNGA 4 cut(s) 93, 850, 1040, 1141
Hpy188III TCNNGA 7 cut(s) 82, 125, 314, 424, 515, 589, 820
Hpy8I GTNNAC 6 cut(s) 243, 317, 460, 560, 888, 953
HpyAV CCTTC 8 cut(s) 60, 421, 425, 803, 853, 1039, 1060, 1204
HpyCH4III ACNGT 3 cut(s) 472, 564, 892
HpyCH4IV ACGT 2 cut(s) 757, 1042
HpyCH4V TGCA 4 cut(s) 10, 560, 569, 920
HpyF10VI GCNNNNNNNGC 9 cut(s) 27, 194, 255, 566, 583, 653, 860, 929, 1109
HpyF3I CTNAG 6 cut(s) 90, 353, 612, 882, 1138, 1194
HpySE526I ACGT 2 cut(s) 757, 1042
Hsp92II CATG 6 cut(s) 151, 774, 800, 994, 1074, 1208
HspAI GCGC 2 cut(s) 907, 1103
KpnI GGTACC 1 cut(s) 471
Kzo9I GATC 5 cut(s) 84, 127, 702, 706, 845
LguI GCTCTTC 1 cut(s) 1053
LmnI GCTCC 1 cut(s) 348
Lsp1109I GCAGC 4 cut(s) 181, 217, 932, 1096
LweI GCATC 3 cut(s) 282, 841, 1159
MaeI CTAG 1 cut(s) 680
MaeII ACGT 2 cut(s) 757, 1042
MaeIII GTNAC 2 cut(s) 748, 835
MalI GATC 5 cut(s) 86, 129, 704, 708, 847
MboI GATC 5 cut(s) 84, 127, 702, 706, 845
MboII GAAGA 8 cut(s) 51, 63, 108, 122, 302, 316, 403, 1040
MfeI CAATTG 1 cut(s) 252
MflI RGATCY 1 cut(s) 845
MhlI GDGCHC 5 cut(s) 345, 562, 588, 1048, 1061
MluCI AATT 3 cut(s) 103, 252, 450
MlyI GAGTC 2 cut(s) 248, 1205
MroXI GAANNNNTTC 1 cut(s) 759
MseI TTAA 2 cut(s) 453, 786
MspA1I CMGCKG 2 cut(s) 616, 1193
MspI CCGG 2 cut(s) 483, 948
MspR9I CCNGG 5 cut(s) 213, 447, 509, 668, 843
MunI CAATTG 1 cut(s) 252
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 5 cut(s) 213, 447, 509, 668, 843
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 9 cut(s) 27, 194, 255, 566, 583, 653, 860, 929, 1109
NdeII GATC 5 cut(s) 84, 127, 702, 706, 845
NlaIII CATG 6 cut(s) 151, 774, 800, 994, 1074, 1208
NlaIV GGNNCC 6 cut(s) 208, 469, 487, 598, 721, 1189
NmuCI GTSAC 1 cut(s) 748
NruI TCGCGA 1 cut(s) 126
NsbI TGCGCA 1 cut(s) 908
NspI RCATGY 2 cut(s) 151, 774
PaeI GCATGC 1 cut(s) 774
PasI CCCWGGG 1 cut(s) 508
PciI ACATGT 1 cut(s) 147
PciSI GCTCTTC 1 cut(s) 1053
PctI GAATGC 1 cut(s) 637
PdmI GAANNNNTTC 1 cut(s) 759
PfeI GAWTC 2 cut(s) 293, 1155
PkrI GCNGC 6 cut(s) 196, 232, 769, 922, 1111, 1192
PleI GAGTC 2 cut(s) 247, 1204
PpsI GAGTC 2 cut(s) 247, 1204
PscI ACATGT 1 cut(s) 147
Psp6I CCWGG 5 cut(s) 211, 445, 507, 666, 841
PspEI GGTNACC 1 cut(s) 835
PspFI CCCAGC 1 cut(s) 528
PspGI CCWGG 5 cut(s) 211, 445, 507, 666, 841
PspN4I GGNNCC 6 cut(s) 208, 469, 487, 598, 721, 1189
PspPI GGNCC 4 cut(s) 596, 719, 781, 1125
PstI CTGCAG 1 cut(s) 922
PstNI CAGNNNCTG 2 cut(s) 848, 929
PsuI RGATCY 1 cut(s) 845
PvuII CAGCTG 1 cut(s) 616
RruI TCGCGA 1 cut(s) 126
RsaI GTAC 5 cut(s) 25, 469, 545, 887, 1223
RsaNI GTAC 5 cut(s) 24, 468, 544, 886, 1222
SapI GCTCTTC 1 cut(s) 1053
SaqAI TTAA 2 cut(s) 453, 786
SatI GCNGC 6 cut(s) 195, 231, 768, 921, 1110, 1191
Sau3AI GATC 5 cut(s) 84, 127, 702, 706, 845
Sau96I GGNCC 4 cut(s) 596, 719, 781, 1125
ScaI AGTACT 2 cut(s) 545, 1223
SchI GAGTC 2 cut(s) 248, 1205
ScrFI CCNGG 5 cut(s) 213, 447, 509, 668, 843
SduI GDGCHC 5 cut(s) 345, 562, 588, 1048, 1061
SfaNI GCATC 3 cut(s) 282, 841, 1159
SfcI CTRYAG 1 cut(s) 918
SinI GGWCC 1 cut(s) 719
SmlI CTYRAG 2 cut(s) 587, 1001
SmoI CTYRAG 2 cut(s) 587, 1001
SphI GCATGC 1 cut(s) 774
Sse9I AATT 3 cut(s) 103, 252, 450
SsiI CCGC 3 cut(s) 396, 768, 1191
SspMI CTAG 1 cut(s) 680
StyD4I CCNGG 5 cut(s) 211, 445, 507, 666, 841
StyI CCWWGG 1 cut(s) 1128
TaaI ACNGT 3 cut(s) 472, 564, 892
TaiI ACGT 2 cut(s) 760, 1045
TaqI TCGA 4 cut(s) 17, 83, 705, 732
TaqII GACCGA 1 cut(s) 767
TasI AATT 3 cut(s) 103, 252, 450
TatI WGTACW 3 cut(s) 543, 885, 1221
TauI GCSGC 2 cut(s) 770, 1193
TfiI GAWTC 2 cut(s) 293, 1155
Tru1I TTAA 2 cut(s) 453, 786
Tru9I TTAA 2 cut(s) 453, 786
TscAI CASTG 2 cut(s) 334, 1068
TseFI GTSAC 1 cut(s) 748
TseI GCWGC 4 cut(s) 194, 230, 920, 1109
Tsp45I GTSAC 1 cut(s) 748
TspDTI ATGAA 2 cut(s) 564, 1007
TspRI CASTG 2 cut(s) 334, 1068
VneI GTGCAC 1 cut(s) 558
VpaK11BI GGWCC 1 cut(s) 719
XagI CCTNNNNNAGG 1 cut(s) 371
XceI RCATGY 2 cut(s) 151, 774
XcmI CCANNNNNNNNNTGG 2 cut(s) 353, 515
XmnI GAANNNNTTC 1 cut(s) 759
XspI CTAG 1 cut(s) 680
ZrmI AGTACT 2 cut(s) 545, 1223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.