MD14G1016700.v1.1

fructose-bisphosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001206
Physical Location & Seq
Forward (+)
1509395 .. 1511350
1956 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1016700.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGTCGGCCTTCAAGGGAAAGTATCACGATGAGCTCATTGCTAATGCTACCTACATTGGTACCCCGGGAAAGGGTATCCTTGCAGCCGATGAGTCAACTGGCACGATTGGCAAGCGTTTTTCCAGCATTGGCGTTGAGAATACTGAGACAAACAGGCGCGCTCTCCGTGAGCTGCTCTTCACCACTCCCGGTGCCGTCCAATACCTCAGCGGAGTCATTCTCTTTGAGGAGACCCTCTACCAAAAGACCGATGCTGGTAAGCCATTCGTTGATGTTTTGAAGGAAGGTGGTGTCCTCCCTGGCATTAAGGTTGACAAGGGCACTGTCGAGCTTGCTGGTACCAATGGTGAAACCACAACTCAGGGTCTTGATGGCCTTGGCGAACGTTGCAAGAAGTACTACGAAGCTGGTGCCCGATTTGCAAAATGGCGTGCCGTGCTGAAGATTGGCCCCAACGAGCCTTCTCAGCTGTCAATCAACGAGAACGCCAATGGGTTGGCCCGATATGCTATCATCTGCCAGGAGAATGGCCTCGTTCCCATTGTTGAGCCAGAGATTCTGGTTGATGGATCCCATGACATTCAAAAGTGTGCTGATGTGACCGAACGTGTTCTTGCAGCATGCTACAAGGCCCTTAACGACCACCATGTCCTGCTTGAAGGAACTCTATTGAAGCCTAACATGGTGACTCCAGGATCTGATGCAAAAAAGGTTCCACCCGAGATGATTGCTGAGTACACTGTCCGTGCCTTGCAGCGCACCATGCCCGCAGCTGTCCCTGCTGTGGTGTTCCTCTCTGGTGGGCAGAGCGAGGAGGAGGCTACCATCAACCTCAACGCTATGAACAAGTTGAAGGCGAATAAGCCATGGTCTCTTTCCTTCTCCTTCGGACGTGCCCTTCAGCAAAGCACTCTCAAGGCTTGGTCAGGAAAGGAAGGGAACGTGAAGAAGGCGCAGGAGGCTTTCCTAACCAGGGCCAAGGCCAACTCGGAAGCGACATTGGGAAACTACAAGGGTGATGCCAAGCTCGGTGAAGGTGCCGCGGAGAGCCTTCACGTCAAGGACTACAAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00680 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00030 map00051 map00680 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
Glycolytic

Protein Analysis

359

Amino Acids

38.61

Weight (kDa)

7.62

Isoelectric Point (pI)

25.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycolytic PF00274 11 - 358 3.6e-175 Fructose-bisphosphate aldolase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 647
Acc65I GGTACC 2 cut(s) 59, 338
AccB1I GGYRCC 5 cut(s) 59, 191, 338, 410, 1037
AccII CGCG 2 cut(s) 159, 1043
AciI CCGC 4 cut(s) 210, 768, 1041, 1043
AclI AACGTT 1 cut(s) 385
AclWI GGATC 3 cut(s) 564, 577, 703
AcuI CTGAAG 2 cut(s) 461, 884
AfaI GTAC 4 cut(s) 61, 340, 398, 737
AfiI CCNNNNNNNGG 4 cut(s) 70, 71, 784, 973
AflIII ACRYGT 1 cut(s) 607
AgsI TTSAA 6 cut(s) 13, 280, 584, 659, 673, 853
AjiI CACGTC 2 cut(s) 893, 1057
AjnI CCWGG 4 cut(s) 298, 519, 691, 971
AluBI AGCT 7 cut(s) 34, 172, 331, 407, 469, 773, 1027
AluI AGCT 7 cut(s) 34, 172, 331, 407, 469, 773, 1027
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 3 cut(s) 140, 224, 876
AlwI GGATC 3 cut(s) 564, 577, 703
AlwNI CAGNNNCTG 1 cut(s) 698
Ama87I CYCGRG 2 cut(s) 64, 719
AoxI GGCC 8 cut(s) 6, 373, 448, 498, 529, 630, 975, 981
ApeKI GCWGC 5 cut(s) 83, 172, 617, 754, 770
Asp700I GAANNNNTTC 1 cut(s) 609
Asp718I GGTACC 2 cut(s) 59, 338
AspLEI GCGC 4 cut(s) 159, 161, 759, 955
AspS9I GGNCC 4 cut(s) 449, 499, 631, 975
AsuC2I CCSGG 3 cut(s) 65, 66, 189
AsuHPI GGTGA 5 cut(s) 172, 359, 697, 1028, 1043
AvaI CYCGRG 2 cut(s) 64, 719
BaeGI GKGCMC 3 cut(s) 323, 415, 898
BaeI ACNNNNGTAYC 2 cut(s) 43, 76
BamHI GGATCC 1 cut(s) 569
BanI GGYRCC 5 cut(s) 59, 191, 338, 410, 1037
BanII GRGCYC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 36
BbvCI CCTCAGC 1 cut(s) 206
BbvI GCAGC 5 cut(s) 95, 159, 629, 766, 782
BccI CCATC 3 cut(s) 365, 560, 833
BceAI ACGGC 2 cut(s) 179, 419
BcgI CGANNNNNNTGC 4 cut(s) 392, 426, 710, 744
BciT130I CCWGG 4 cut(s) 300, 521, 693, 973
BciVI GTATCC 1 cut(s) 86
BcnI CCSGG 3 cut(s) 65, 66, 189
BcoDI GTCTC 3 cut(s) 140, 224, 876
BfuI GTATCC 1 cut(s) 86
BisI GCNGC 6 cut(s) 84, 173, 618, 755, 771, 1041
BlsI GCNGC 6 cut(s) 85, 174, 619, 756, 772, 1042
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 7 cut(s) 65, 66, 189, 300, 521, 693, 973
BmeT110I CYCGRG 2 cut(s) 64, 719
BmgBI CACGTC 2 cut(s) 893, 1057
BmgT120I GGNCC 4 cut(s) 449, 499, 631, 975
BmiI GGNNCC 8 cut(s) 61, 193, 340, 412, 451, 571, 714, 1039
BmrFI CCNGG 7 cut(s) 65, 66, 189, 300, 521, 693, 973
BmsI GCATC 3 cut(s) 241, 691, 1009
BplI GAGNNNNNCTC 2 cut(s) 204, 236
BpmI CTGGAG 1 cut(s) 675
Bpu10I CCTNAGC 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 3 cut(s) 65, 66, 189
BsaI GGTCTC 2 cut(s) 224, 876
BsaJI CCNNGG 8 cut(s) 63, 64, 298, 376, 866, 972, 978, 1041
Bsc4I CCNNNNNNNGG 4 cut(s) 70, 71, 784, 973
Bse1I ACTGG 1 cut(s) 103
Bse3DI GCAATG 1 cut(s) 36
BseBI CCWGG 4 cut(s) 300, 521, 693, 973
BseDI CCNNGG 8 cut(s) 63, 64, 298, 376, 866, 972, 978, 1041
BseLI CCNNNNNNNGG 4 cut(s) 70, 71, 784, 973
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 5 cut(s) 135, 220, 374, 479, 723
BseNI ACTGG 1 cut(s) 103
BsePI GCGCGC 1 cut(s) 157
BseRI GAGGAG 3 cut(s) 242, 827, 830
BseSI GKGCMC 3 cut(s) 323, 415, 898
BseXI GCAGC 5 cut(s) 95, 159, 629, 766, 782
Bsh1236I CGCG 2 cut(s) 159, 1043
BshFI GGCC 8 cut(s) 8, 375, 450, 500, 531, 632, 977, 983
BshNI GGYRCC 5 cut(s) 59, 191, 338, 410, 1037
BsiHKAI GWGCWC 1 cut(s) 36
BsiHKCI CYCGRG 2 cut(s) 64, 719
BsiSI CCGG 2 cut(s) 65, 189
BslFI GGGAC 1 cut(s) 761
BslI CCNNNNNNNGG 4 cut(s) 70, 71, 784, 973
BsmAI GTCTC 3 cut(s) 140, 224, 876
BsmFI GGGAC 1 cut(s) 761
BsnI GGCC 8 cut(s) 8, 375, 450, 500, 531, 632, 977, 983
Bso31I GGTCTC 2 cut(s) 224, 876
BsoBI CYCGRG 2 cut(s) 64, 719
Bsp1286I GDGCHC 4 cut(s) 36, 323, 415, 898
Bsp143I GATC 2 cut(s) 569, 695
Bsp19I CCATGG 1 cut(s) 866
BspACI CCGC 4 cut(s) 210, 768, 1041, 1043
BspANI GGCC 8 cut(s) 8, 375, 450, 500, 531, 632, 977, 983
BspCNI CTCAG 5 cut(s) 136, 219, 373, 478, 724
BspFNI CGCG 2 cut(s) 159, 1043
BspLI GGNNCC 8 cut(s) 61, 193, 340, 412, 451, 571, 714, 1039
BspPI GGATC 3 cut(s) 564, 577, 703
BspQI GCTCTTC 1 cut(s) 182
BspT107I GGYRCC 5 cut(s) 59, 191, 338, 410, 1037
BspTNI GGTCTC 2 cut(s) 224, 876
BsrDI GCAATG 1 cut(s) 36
BsrI ACTGG 1 cut(s) 103
BssECI CCNNGG 8 cut(s) 63, 64, 298, 376, 866, 972, 978, 1041
BssHII GCGCGC 1 cut(s) 157
BssMI GATC 2 cut(s) 569, 695
BssT1I CCWWGG 3 cut(s) 376, 866, 978
Bst2UI CCWGG 4 cut(s) 300, 521, 693, 973
Bst4CI ACNGT 2 cut(s) 325, 742
Bst6I CTCTTC 1 cut(s) 182
BstC8I GCNNGC 6 cut(s) 113, 159, 333, 432, 622, 768
BstDEI CTNAG 5 cut(s) 144, 206, 360, 465, 732
BstDSI CCRYGG 2 cut(s) 866, 1041
BstFNI CGCG 2 cut(s) 159, 1043
BstHHI GCGC 4 cut(s) 159, 161, 759, 955
BstKTI GATC 2 cut(s) 572, 698
BstMAI GTCTC 3 cut(s) 140, 224, 876
BstMBI GATC 2 cut(s) 569, 695
BstMWI GCNNNNNNNGC 9 cut(s) 108, 387, 419, 436, 466, 506, 763, 779, 959
BstNI CCWGG 4 cut(s) 300, 521, 693, 973
BstNSI RCATGY 1 cut(s) 624
BstSCI CCNGG 7 cut(s) 63, 64, 187, 298, 519, 691, 971
BstSLI GKGCMC 3 cut(s) 323, 415, 898
BstUI CGCG 2 cut(s) 159, 1043
BstV1I GCAGC 5 cut(s) 95, 159, 629, 766, 782
BstX2I RGATCY 2 cut(s) 569, 695
BstXI CCANNNNNNTGG 2 cut(s) 496, 527
BstYI RGATCY 2 cut(s) 569, 695
BsuI GTATCC 1 cut(s) 86
BsuRI GGCC 8 cut(s) 8, 375, 450, 500, 531, 632, 977, 983
BtgI CCRYGG 2 cut(s) 866, 1041
BtrI CACGTC 2 cut(s) 893, 1057
BtsIMutI CAGTG 2 cut(s) 321, 738
Cac8I GCNNGC 6 cut(s) 113, 159, 333, 432, 622, 768
CaiI CAGNNNCTG 1 cut(s) 698
CfoI GCGC 4 cut(s) 159, 161, 759, 955
Cfr13I GGNCC 4 cut(s) 449, 499, 631, 975
Cfr42I CCGCGG 1 cut(s) 1044
Cfr9I CCCGGG 1 cut(s) 64
Csp6I GTAC 4 cut(s) 60, 339, 397, 736
CviAII CATG 6 cut(s) 575, 621, 647, 682, 763, 867
CviQI GTAC 4 cut(s) 60, 339, 397, 736
DdeI CTNAG 5 cut(s) 144, 206, 360, 465, 732
DpnI GATC 2 cut(s) 571, 697
DpnII GATC 2 cut(s) 569, 695
DrdI GACNNNNNNGTC 1 cut(s) 647
DseDI GACNNNNNNGTC 1 cut(s) 647
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Ecl136II GAGCTC 1 cut(s) 34
Eco130I CCWWGG 3 cut(s) 376, 866, 978
Eco24I GRGCYC 1 cut(s) 36
Eco31I GGTCTC 2 cut(s) 224, 876
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 2 cut(s) 461, 884
Eco88I CYCGRG 2 cut(s) 64, 719
EcoICRI GAGCTC 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 631
EcoRII CCWGG 4 cut(s) 298, 519, 691, 971
EcoT14I CCWWGG 3 cut(s) 376, 866, 978
EcoT38I GRGCYC 1 cut(s) 36
ErhI CCWWGG 3 cut(s) 376, 866, 978
FaeI CATG 6 cut(s) 578, 624, 650, 685, 766, 870
FaiI YATR 8 cut(s) 507, 576, 622, 648, 683, 764, 842, 868
FaqI GGGAC 1 cut(s) 761
FatI CATG 6 cut(s) 574, 620, 646, 681, 762, 866
FauI CCCGC 1 cut(s) 775
Fnu4HI GCNGC 6 cut(s) 84, 173, 618, 755, 771, 1041
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 6 cut(s) 84, 173, 618, 755, 771, 1041
GlaI GCGC 4 cut(s) 158, 160, 758, 954
GluI GCNGC 6 cut(s) 84, 173, 618, 755, 771, 1041
GsuI CTGGAG 1 cut(s) 675
HaeIII GGCC 8 cut(s) 8, 375, 450, 500, 531, 632, 977, 983
HapII CCGG 2 cut(s) 65, 189
HhaI GCGC 4 cut(s) 159, 161, 759, 955
Hin1II CATG 6 cut(s) 578, 624, 650, 685, 766, 870
Hin6I GCGC 4 cut(s) 157, 159, 757, 953
HinP1I GCGC 4 cut(s) 157, 159, 757, 953
HincII GTYRAC 2 cut(s) 96, 313
HindII GTYRAC 2 cut(s) 96, 313
HinfI GANTC 4 cut(s) 92, 213, 556, 688
HpaII CCGG 2 cut(s) 65, 189
HphI GGTGA 5 cut(s) 172, 359, 697, 1028, 1043
Hpy166II GTNNAC 3 cut(s) 96, 313, 738
Hpy188I TCNGA 3 cut(s) 700, 890, 991
Hpy188III TCNNGA 3 cut(s) 26, 368, 927
Hpy8I GTNNAC 3 cut(s) 96, 313, 738
HpyCH4III ACNGT 2 cut(s) 325, 742
HpyCH4IV ACGT 5 cut(s) 385, 607, 892, 942, 1056
HpyCH4V TGCA 6 cut(s) 83, 390, 422, 617, 704, 754
HpyF10VI GCNNNNNNNGC 9 cut(s) 108, 387, 419, 436, 466, 506, 763, 779, 959
HpyF3I CTNAG 5 cut(s) 144, 206, 360, 465, 732
HpySE526I ACGT 5 cut(s) 385, 607, 892, 942, 1056
Hsp92II CATG 6 cut(s) 578, 624, 650, 685, 766, 870
HspAI GCGC 4 cut(s) 157, 159, 757, 953
KpnI GGTACC 2 cut(s) 63, 342
KspI CCGCGG 1 cut(s) 1044
Kzo9I GATC 2 cut(s) 569, 695
LguI GCTCTTC 1 cut(s) 182
Lsp1109I GCAGC 5 cut(s) 95, 159, 629, 766, 782
LweI GCATC 3 cut(s) 241, 691, 1009
MaeII ACGT 5 cut(s) 385, 607, 892, 942, 1056
MaeIII GTNAC 2 cut(s) 598, 685
MalI GATC 2 cut(s) 571, 697
MboI GATC 2 cut(s) 569, 695
MboII GAAGA 3 cut(s) 169, 454, 958
MflI RGATCY 2 cut(s) 569, 695
MhlI GDGCHC 4 cut(s) 36, 323, 415, 898
MlyI GAGTC 3 cut(s) 101, 222, 682
MroXI GAANNNNTTC 1 cut(s) 609
MseI TTAA 2 cut(s) 306, 636
MspA1I CMGCKG 4 cut(s) 210, 469, 773, 1043
MspI CCGG 2 cut(s) 65, 189
MspR9I CCNGG 7 cut(s) 65, 66, 189, 300, 521, 693, 973
MvaI CCWGG 4 cut(s) 300, 521, 693, 973
MvnI CGCG 2 cut(s) 159, 1043
MwoI GCNNNNNNNGC 9 cut(s) 108, 387, 419, 436, 466, 506, 763, 779, 959
NciI CCSGG 3 cut(s) 65, 66, 189
NcoI CCATGG 1 cut(s) 866
NdeII GATC 2 cut(s) 569, 695
NlaIII CATG 6 cut(s) 578, 624, 650, 685, 766, 870
NlaIV GGNNCC 8 cut(s) 61, 193, 340, 412, 451, 571, 714, 1039
NmuCI GTSAC 2 cut(s) 598, 685
NspI RCATGY 1 cut(s) 624
PaeI GCATGC 1 cut(s) 624
PauI GCGCGC 1 cut(s) 157
PciSI GCTCTTC 1 cut(s) 182
PdmI GAANNNNTTC 1 cut(s) 609
PfeI GAWTC 1 cut(s) 556
PfoI TCCNGGA 1 cut(s) 691
PkrI GCNGC 6 cut(s) 85, 174, 619, 756, 772, 1042
PleI GAGTC 3 cut(s) 100, 221, 682
PpsI GAGTC 3 cut(s) 100, 221, 682
Psp124BI GAGCTC 1 cut(s) 36
Psp1406I AACGTT 1 cut(s) 385
Psp6I CCWGG 4 cut(s) 298, 519, 691, 971
PspGI CCWGG 4 cut(s) 298, 519, 691, 971
PspN4I GGNNCC 8 cut(s) 61, 193, 340, 412, 451, 571, 714, 1039
PspPI GGNCC 4 cut(s) 449, 499, 631, 975
PstNI CAGNNNCTG 1 cut(s) 698
PsuI RGATCY 2 cut(s) 569, 695
PteI GCGCGC 1 cut(s) 157
PvuII CAGCTG 2 cut(s) 469, 773
RsaI GTAC 4 cut(s) 61, 340, 398, 737
RsaNI GTAC 4 cut(s) 60, 339, 397, 736
SacI GAGCTC 1 cut(s) 36
SacII CCGCGG 1 cut(s) 1044
SapI GCTCTTC 1 cut(s) 182
SaqAI TTAA 2 cut(s) 306, 636
SatI GCNGC 6 cut(s) 84, 173, 618, 755, 771, 1041
Sau3AI GATC 2 cut(s) 569, 695
Sau96I GGNCC 4 cut(s) 449, 499, 631, 975
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 3 cut(s) 101, 222, 682
ScrFI CCNGG 7 cut(s) 65, 66, 189, 300, 521, 693, 973
SduI GDGCHC 4 cut(s) 36, 323, 415, 898
SfaNI GCATC 3 cut(s) 241, 691, 1009
Sfr303I CCGCGG 1 cut(s) 1044
SgrBI CCGCGG 1 cut(s) 1044
SmaI CCCGGG 1 cut(s) 66
SmlI CTYRAG 1 cut(s) 914
SmoI CTYRAG 1 cut(s) 914
SphI GCATGC 1 cut(s) 624
SsiI CCGC 4 cut(s) 210, 768, 1041, 1043
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 7 cut(s) 63, 64, 187, 298, 519, 691, 971
StyI CCWWGG 3 cut(s) 376, 866, 978
TaaI ACNGT 2 cut(s) 325, 742
TaiI ACGT 5 cut(s) 388, 610, 895, 945, 1059
TaqI TCGA 1 cut(s) 327
TaqII GACCGA 2 cut(s) 263, 617
TatI WGTACW 2 cut(s) 396, 735
TauI GCSGC 1 cut(s) 1043
TfiI GAWTC 1 cut(s) 556
Tru1I TTAA 2 cut(s) 306, 636
Tru9I TTAA 2 cut(s) 306, 636
TscAI CASTG 2 cut(s) 328, 745
TseFI GTSAC 2 cut(s) 598, 685
TseI GCWGC 5 cut(s) 83, 172, 617, 754, 770
Tsp45I GTSAC 2 cut(s) 598, 685
TspDTI ATGAA 1 cut(s) 857
TspGWI ACGGA 2 cut(s) 155, 734
TspMI CCCGGG 1 cut(s) 64
TspRI CASTG 2 cut(s) 328, 745
XceI RCATGY 1 cut(s) 624
XmaI CCCGGG 1 cut(s) 64
XmnI GAANNNNTTC 1 cut(s) 609
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.