Rh3AG175600

fructose-bisphosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001206
Physical Location & Seq
Forward (+)
16349436 .. 16352579
3144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG175600.1

Sequence Viewer

Length: 1077 bp
ATGACTGCTTACAGAGGCAAATACGCTGATGAGCTCATTGCCAATGCTGCCTACATTGGCACCCCTGGAAAGGGTATTCTGGCTGCTGATGAGTCCACTGGCACCATTGGAAAACGTTTTTCCAGCATCAATGTTGAGAATGTTGAAGAGAACAGGCGTGCTCTTCGTGAGCTCCTCTTCACCGCCCCCAATGTCCTCCAGTACCTTAGTGGAGTGATCCTCTTTGAGGAAACCCTCTACCAGAAAACAGCTGCAGGCAAGCCTTTCGTTGATGTCTTGAAGGAAGGTGGTGTTCTTCCTGGTATCAAGGTTGACAAGGGTGTTGTTGAGCTTAAAGGCACCGACGGTGAGACCACAACCCAGGGTTTGGATGGCCTTGGTGCCCGCTGCGCTAAGTACTATGAAGCCGGTGCCCGGTTTGCCAAATGGCGTGCTGTGCTCAAGATTGGTCCCAATGAGCCATCTCAGCTTTCAATCAACGAGAATGCCAATGGCTTGGCTCGTTATGCTATCATCTGCCAGGAGAATGGTTTGGTACCCATTGTTGAGCCTGAGATCCTTGTTGACGGACCCCACTCCATTGACAAGTGTGCTGATGTGACTGAGCGCGTCCTTGCTGCATGTTACAAGGCTCTCAATGACCACCACGTCCTTCTTGAAGGAACTCTCTTGAAGCCCAACATGGTGACTCCCGGATCTGATGCCAAGAAGGCTTCACCAGAAGTGATTGCTGAGTACACCGTCCGTGCCTTGCAGCGCACTACCCCTGCAGCTGTCCCTGCTGTGGTGTTCTTGTCTGGTGGACAGAGTGAGGAGGAGGCTACTCTCAACCTTAACGCCATGAACAAGCTCAAGGGAAAGAAGCCATGGACTCTTTCCTTCTCCTTCGGCCGTGCTCTTCAAGCCAGCACTCTCAAGGCTTGGTCAGGAAAGAAAGAAAATGTTAAGGCTGCACAGGAGGCTCTCCTCACAAGGGCCAAGGCCAACTCAGAGGCAACCTTGGGAACCTACAAGGGTGATGCCAAGCTCGGAGAGGGTGCTGCAGAGAGCCTTCACGTCAAGGACTACAAGTACTGA

Protein Analysis

358

Amino Acids

38.41

Weight (kDa)

8.15

Isoelectric Point (pI)

23.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycolytic PF00274 11 - 358 6.1e-175 Fructose-bisphosphate aldolase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 647
Acc65I GGTACC 1 cut(s) 535
AccB1I GGYRCC 6 cut(s) 59, 101, 338, 380, 410, 535
AccB7I CCANNNNNTGG 1 cut(s) 367
AccII CGCG 1 cut(s) 609
AciI CCGC 2 cut(s) 183, 385
AclI AACGTT 1 cut(s) 115
AclWI GGATC 3 cut(s) 211, 550, 703
AcoI YGGCCR 1 cut(s) 889
AfaI GTAC 5 cut(s) 203, 398, 537, 737, 1073
AfiI CCNNNNNNNGG 7 cut(s) 70, 71, 226, 367, 414, 784, 973
AgsI TTSAA 6 cut(s) 146, 280, 474, 659, 673, 902
AjiI CACGTC 2 cut(s) 649, 1057
AjnI CCWGG 4 cut(s) 64, 298, 360, 519
AluBI AGCT 8 cut(s) 34, 172, 251, 331, 469, 773, 850, 1027
AluI AGCT 8 cut(s) 34, 172, 251, 331, 469, 773, 850, 1027
Alw21I GWGCWC 5 cut(s) 36, 163, 174, 441, 898
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 3 cut(s) 211, 550, 703
AoxI GGCC 4 cut(s) 373, 889, 975, 981
ApeKI GCWGC 9 cut(s) 47, 83, 251, 387, 617, 754, 770, 950, 1040
Asp718I GGTACC 1 cut(s) 535
AspLEI GCGC 3 cut(s) 392, 609, 759
AspS9I GGNCC 3 cut(s) 449, 569, 975
AsuC2I CCSGG 2 cut(s) 415, 693
AsuHPI GGTGA 5 cut(s) 172, 359, 697, 708, 1028
AvaII GGWCC 2 cut(s) 449, 569
BaeGI GKGCMC 2 cut(s) 385, 415
BanI GGYRCC 6 cut(s) 59, 101, 338, 380, 410, 535
BanII GRGCYC 2 cut(s) 36, 174
Bbv12I GWGCWC 5 cut(s) 36, 163, 174, 441, 898
BbvI GCAGC 9 cut(s) 34, 70, 238, 374, 604, 766, 782, 937, 1027
BccI CCATC 2 cut(s) 365, 469
BceAI ACGGC 1 cut(s) 876
BcgI CGANNNNNNTGC 2 cut(s) 247, 281
BciT130I CCWGG 4 cut(s) 66, 300, 362, 521
BcnI CCSGG 2 cut(s) 415, 693
BcoDI GTCTC 1 cut(s) 344
BfmI CTRYAG 3 cut(s) 252, 768, 1041
BglI GCCNNNNNGGC 1 cut(s) 710
BisI GCNGC 9 cut(s) 48, 84, 252, 388, 618, 755, 771, 951, 1041
BlsI GCNGC 9 cut(s) 49, 85, 253, 389, 619, 756, 772, 952, 1042
BmcAI AGTACT 2 cut(s) 398, 1073
Bme1390I CCNGG 6 cut(s) 66, 300, 362, 415, 521, 693
Bme18I GGWCC 2 cut(s) 449, 569
BmgBI CACGTC 2 cut(s) 649, 1057
BmgT120I GGNCC 3 cut(s) 449, 569, 975
BmiI GGNNCC 9 cut(s) 61, 103, 340, 382, 412, 451, 537, 571, 1006
BmrFI CCNGG 6 cut(s) 66, 300, 362, 415, 521, 693
BmsI GCATC 3 cut(s) 135, 691, 1009
BplI GAGNNNNNCTC 2 cut(s) 204, 236
BpmI CTGGAG 1 cut(s) 182
BpuEI CTTGAG 3 cut(s) 425, 836, 899
BpuMI CCSGG 2 cut(s) 415, 693
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 7 cut(s) 64, 360, 361, 376, 866, 978, 999
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 71, 226, 367, 414, 784, 973
Bse118I RCCGGY 1 cut(s) 407
Bse1I ACTGG 2 cut(s) 103, 199
Bse3DI GCAATG 1 cut(s) 36
BseBI CCWGG 4 cut(s) 66, 300, 362, 521
BseDI CCNNGG 7 cut(s) 64, 360, 361, 376, 866, 978, 999
BseGI GGATG 1 cut(s) 376
BseLI CCNNNNNNNGG 7 cut(s) 70, 71, 226, 367, 414, 784, 973
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 5 cut(s) 479, 543, 594, 723, 1002
BseNI ACTGG 2 cut(s) 103, 199
BseRI GAGGAG 4 cut(s) 164, 827, 830, 956
BseSI GKGCMC 2 cut(s) 385, 415
BseX3I CGGCCG 1 cut(s) 889
BseXI GCAGC 9 cut(s) 34, 70, 238, 374, 604, 766, 782, 937, 1027
BsgI GTGCAG 1 cut(s) 936
Bsh1236I CGCG 1 cut(s) 609
Bsh1285I CGRYCG 1 cut(s) 892
BshFI GGCC 4 cut(s) 375, 891, 977, 983
BshNI GGYRCC 6 cut(s) 59, 101, 338, 380, 410, 535
BsiEI CGRYCG 1 cut(s) 892
BsiHKAI GWGCWC 5 cut(s) 36, 163, 174, 441, 898
BsiSI CCGG 3 cut(s) 408, 415, 693
BslFI GGGAC 2 cut(s) 435, 761
BslI CCNNNNNNNGG 7 cut(s) 70, 71, 226, 367, 414, 784, 973
BsmAI GTCTC 1 cut(s) 344
BsmFI GGGAC 2 cut(s) 435, 761
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 4 cut(s) 375, 891, 977, 983
Bso31I GGTCTC 1 cut(s) 344
Bsp1286I GDGCHC 7 cut(s) 36, 163, 174, 385, 415, 441, 898
Bsp143I GATC 3 cut(s) 216, 555, 695
Bsp19I CCATGG 1 cut(s) 866
BspACI CCGC 2 cut(s) 183, 385
BspANI GGCC 4 cut(s) 375, 891, 977, 983
BspCNI CTCAG 5 cut(s) 478, 544, 595, 724, 1001
BspFNI CGCG 1 cut(s) 609
BspLI GGNNCC 9 cut(s) 61, 103, 340, 382, 412, 451, 537, 571, 1006
BspMAI CTGCAG 3 cut(s) 256, 772, 1045
BspPI GGATC 3 cut(s) 211, 550, 703
BspQI GCTCTTC 2 cut(s) 168, 903
BspT107I GGYRCC 6 cut(s) 59, 101, 338, 380, 410, 535
BspTNI GGTCTC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 36
BsrFI RCCGGY 1 cut(s) 407
BsrI ACTGG 2 cut(s) 103, 199
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 7 cut(s) 64, 360, 361, 376, 866, 978, 999
BssMI GATC 3 cut(s) 216, 555, 695
BssT1I CCWWGG 4 cut(s) 376, 866, 978, 999
Bst2UI CCWGG 4 cut(s) 66, 300, 362, 521
Bst4CI ACNGT 2 cut(s) 347, 742
Bst6I CTCTTC 4 cut(s) 141, 168, 182, 903
BstC8I GCNNGC 6 cut(s) 159, 256, 260, 385, 432, 907
BstDEI CTNAG 7 cut(s) 206, 393, 465, 552, 603, 732, 988
BstDSI CCRYGG 1 cut(s) 866
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 1 cut(s) 376
BstFNI CGCG 1 cut(s) 609
BstHHI GCGC 3 cut(s) 392, 609, 759
BstKTI GATC 3 cut(s) 219, 558, 698
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 3 cut(s) 216, 555, 695
BstMCI CGRYCG 1 cut(s) 892
BstNI CCWGG 4 cut(s) 66, 300, 362, 521
BstNSI RCATGY 1 cut(s) 624
BstSCI CCNGG 6 cut(s) 64, 298, 360, 413, 519, 691
BstSFI CTRYAG 3 cut(s) 252, 768, 1041
BstSLI GKGCMC 2 cut(s) 385, 415
BstUI CGCG 1 cut(s) 609
BstV1I GCAGC 9 cut(s) 34, 70, 238, 374, 604, 766, 782, 937, 1027
BstX2I RGATCY 2 cut(s) 555, 695
BstXI CCANNNNNNTGG 2 cut(s) 496, 527
BstYI RGATCY 2 cut(s) 555, 695
BstZI CGGCCG 1 cut(s) 889
BsuRI GGCC 4 cut(s) 375, 891, 977, 983
BtgI CCRYGG 1 cut(s) 866
BtrI CACGTC 2 cut(s) 649, 1057
BtsCI GGATG 1 cut(s) 376
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 6 cut(s) 159, 256, 260, 385, 432, 907
CfoI GCGC 3 cut(s) 392, 609, 759
Cfr10I RCCGGY 1 cut(s) 407
Cfr13I GGNCC 3 cut(s) 449, 569, 975
CseI GACGC 1 cut(s) 598
Csp6I GTAC 5 cut(s) 202, 397, 536, 736, 1072
CviAII CATG 4 cut(s) 621, 682, 841, 867
CviQI GTAC 5 cut(s) 202, 397, 536, 736, 1072
DdeI CTNAG 7 cut(s) 206, 393, 465, 552, 603, 732, 988
DpnI GATC 3 cut(s) 218, 557, 697
DpnII GATC 3 cut(s) 216, 555, 695
DrdI GACNNNNNNGTC 1 cut(s) 647
DseDI GACNNNNNNGTC 1 cut(s) 647
EaeI YGGCCR 1 cut(s) 889
EagI CGGCCG 1 cut(s) 889
Eam1104I CTCTTC 4 cut(s) 141, 168, 182, 903
EarI CTCTTC 4 cut(s) 141, 168, 182, 903
Ecl136II GAGCTC 2 cut(s) 34, 172
EclXI CGGCCG 1 cut(s) 889
Eco130I CCWWGG 4 cut(s) 376, 866, 978, 999
Eco24I GRGCYC 2 cut(s) 36, 174
Eco31I GGTCTC 1 cut(s) 344
Eco47I GGWCC 2 cut(s) 449, 569
Eco52I CGGCCG 1 cut(s) 889
Eco53kI GAGCTC 2 cut(s) 34, 172
EcoICRI GAGCTC 2 cut(s) 34, 172
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoRII CCWGG 4 cut(s) 64, 298, 360, 519
EcoT14I CCWWGG 4 cut(s) 376, 866, 978, 999
EcoT38I GRGCYC 2 cut(s) 36, 174
ErhI CCWWGG 4 cut(s) 376, 866, 978, 999
FaeI CATG 4 cut(s) 624, 685, 844, 870
FaiI YATR 6 cut(s) 402, 507, 622, 683, 842, 868
FaqI GGGAC 2 cut(s) 435, 761
FatI CATG 4 cut(s) 620, 681, 840, 866
FauI CCCGC 1 cut(s) 392
Fnu4HI GCNGC 9 cut(s) 48, 84, 252, 388, 618, 755, 771, 951, 1041
FokI GGATG 1 cut(s) 383
FriOI GRGCYC 2 cut(s) 36, 174
Fsp4HI GCNGC 9 cut(s) 48, 84, 252, 388, 618, 755, 771, 951, 1041
GlaI GCGC 3 cut(s) 391, 608, 758
GluI GCNGC 9 cut(s) 48, 84, 252, 388, 618, 755, 771, 951, 1041
GsuI CTGGAG 1 cut(s) 182
HaeIII GGCC 4 cut(s) 375, 891, 977, 983
HapII CCGG 3 cut(s) 408, 415, 693
HgaI GACGC 1 cut(s) 598
HhaI GCGC 3 cut(s) 392, 609, 759
Hin1II CATG 4 cut(s) 624, 685, 844, 870
Hin6I GCGC 3 cut(s) 390, 607, 757
HinP1I GCGC 3 cut(s) 390, 607, 757
HincII GTYRAC 2 cut(s) 313, 565
HindII GTYRAC 2 cut(s) 313, 565
HinfI GANTC 3 cut(s) 92, 688, 871
HpaII CCGG 3 cut(s) 408, 415, 693
HphI GGTGA 5 cut(s) 172, 359, 697, 708, 1028
Hpy166II GTNNAC 5 cut(s) 96, 313, 565, 738, 803
Hpy188I TCNGA 3 cut(s) 700, 991, 1031
Hpy188III TCNNGA 6 cut(s) 167, 277, 442, 656, 670, 927
Hpy8I GTNNAC 5 cut(s) 96, 313, 565, 738, 803
Hpy99I CGWCG 1 cut(s) 347
HpyAV CCTTC 8 cut(s) 274, 278, 653, 662, 703, 889, 895, 1061
HpyCH4III ACNGT 2 cut(s) 347, 742
HpyCH4IV ACGT 3 cut(s) 115, 648, 1056
HpyCH4V TGCA 6 cut(s) 254, 620, 754, 770, 953, 1043
HpyF3I CTNAG 7 cut(s) 206, 393, 465, 552, 603, 732, 988
HpySE526I ACGT 3 cut(s) 115, 648, 1056
Hsp92II CATG 4 cut(s) 624, 685, 844, 870
HspAI GCGC 3 cut(s) 390, 607, 757
KpnI GGTACC 1 cut(s) 539
Kzo9I GATC 3 cut(s) 216, 555, 695
LguI GCTCTTC 2 cut(s) 168, 903
LmnI GCTCC 1 cut(s) 177
Lsp1109I GCAGC 9 cut(s) 34, 70, 238, 374, 604, 766, 782, 937, 1027
LweI GCATC 3 cut(s) 135, 691, 1009
MaeII ACGT 3 cut(s) 115, 648, 1056
MaeIII GTNAC 3 cut(s) 598, 623, 685
MalI GATC 3 cut(s) 218, 557, 697
MboI GATC 3 cut(s) 216, 555, 695
MboII GAAGA 5 cut(s) 155, 158, 169, 287, 890
MflI RGATCY 2 cut(s) 555, 695
MhlI GDGCHC 7 cut(s) 36, 163, 174, 385, 415, 441, 898
MlyI GAGTC 3 cut(s) 101, 682, 865
MseI TTAA 3 cut(s) 333, 834, 945
MspA1I CMGCKG 3 cut(s) 251, 387, 773
MspI CCGG 3 cut(s) 408, 415, 693
MspR9I CCNGG 6 cut(s) 66, 300, 362, 415, 521, 693
Mva1269I GAATGC 1 cut(s) 490
MvaI CCWGG 4 cut(s) 66, 300, 362, 521
MvnI CGCG 1 cut(s) 609
NciI CCSGG 2 cut(s) 415, 693
NcoI CCATGG 1 cut(s) 866
NdeII GATC 3 cut(s) 216, 555, 695
NlaIII CATG 4 cut(s) 624, 685, 844, 870
NlaIV GGNNCC 9 cut(s) 61, 103, 340, 382, 412, 451, 537, 571, 1006
NmuCI GTSAC 2 cut(s) 598, 685
NspI RCATGY 1 cut(s) 624
PasI CCCWGGG 1 cut(s) 361
PciSI GCTCTTC 2 cut(s) 168, 903
PctI GAATGC 1 cut(s) 490
PflMI CCANNNNNTGG 1 cut(s) 367
PfoI TCCNGGA 1 cut(s) 691
PkrI GCNGC 9 cut(s) 49, 85, 253, 389, 619, 756, 772, 952, 1042
PleI GAGTC 3 cut(s) 100, 682, 865
PpsI GAGTC 3 cut(s) 100, 682, 865
Psp124BI GAGCTC 2 cut(s) 36, 174
Psp1406I AACGTT 1 cut(s) 115
Psp6I CCWGG 4 cut(s) 64, 298, 360, 519
PspGI CCWGG 4 cut(s) 64, 298, 360, 519
PspN4I GGNNCC 9 cut(s) 61, 103, 340, 382, 412, 451, 537, 571, 1006
PspPI GGNCC 3 cut(s) 449, 569, 975
PstI CTGCAG 3 cut(s) 256, 772, 1045
PsuI RGATCY 2 cut(s) 555, 695
PvuII CAGCTG 2 cut(s) 251, 773
RsaI GTAC 5 cut(s) 203, 398, 537, 737, 1073
RsaNI GTAC 5 cut(s) 202, 397, 536, 736, 1072
SacI GAGCTC 2 cut(s) 36, 174
SapI GCTCTTC 2 cut(s) 168, 903
SaqAI TTAA 3 cut(s) 333, 834, 945
SatI GCNGC 9 cut(s) 48, 84, 252, 388, 618, 755, 771, 951, 1041
Sau3AI GATC 3 cut(s) 216, 555, 695
Sau96I GGNCC 3 cut(s) 449, 569, 975
ScaI AGTACT 2 cut(s) 398, 1073
SchI GAGTC 3 cut(s) 101, 682, 865
ScrFI CCNGG 6 cut(s) 66, 300, 362, 415, 521, 693
SduI GDGCHC 7 cut(s) 36, 163, 174, 385, 415, 441, 898
SfaNI GCATC 3 cut(s) 135, 691, 1009
SfcI CTRYAG 3 cut(s) 252, 768, 1041
SinI GGWCC 2 cut(s) 449, 569
SmlI CTYRAG 3 cut(s) 440, 851, 914
SmoI CTYRAG 3 cut(s) 440, 851, 914
SsiI CCGC 2 cut(s) 183, 385
SstI GAGCTC 2 cut(s) 36, 174
StyD4I CCNGG 6 cut(s) 64, 298, 360, 413, 519, 691
StyI CCWWGG 4 cut(s) 376, 866, 978, 999
TaaI ACNGT 2 cut(s) 347, 742
TaiI ACGT 3 cut(s) 118, 651, 1059
TatI WGTACW 3 cut(s) 396, 735, 1071
Tru1I TTAA 3 cut(s) 333, 834, 945
Tru9I TTAA 3 cut(s) 333, 834, 945
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 2 cut(s) 598, 685
TseI GCWGC 9 cut(s) 47, 83, 251, 387, 617, 754, 770, 950, 1040
Tsp45I GTSAC 2 cut(s) 598, 685
TspDTI ATGAA 2 cut(s) 417, 857
TspGWI ACGGA 2 cut(s) 582, 734
TspRI CASTG 1 cut(s) 103
Van91I CCANNNNNTGG 1 cut(s) 367
VpaK11BI GGWCC 2 cut(s) 449, 569
XagI CCTNNNNNAGG 1 cut(s) 224
XceI RCATGY 1 cut(s) 624
XcmI CCANNNNNNNNNTGG 2 cut(s) 206, 368
ZrmI AGTACT 2 cut(s) 398, 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.