Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0008865

Physical Location & Seq

Genomic Coordinates Reverse (-)

12351352 .. 12353693

Gene Structure 2342 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g18740.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1611 bp

ATGTCAGAAAAAATATTGATCGTGCTCCATCTTCTGGTATCTTATATAGTTGAATGCTTTTCTCATCTTAGTCGATACCTCAGATCCAAGAAACCCACTAATTATCAGAAGAAGAAGAAGAAGATGGATCAGTCGGACAAATTAAGCAACTTACCAACCGGTGTTACACAGAAAATCTTGTCATACTTACCTATCGACCAGGCAGTGAGGACAAGTATTTTATCTAGTGAGTGGAGGTCGACATGGTCTCGGCTTCCATATCTGGTGTTTGATGATCAAAGTGGATATGATATCAAAAAAGTTGTTAATCATGTGCTGTTACTTCATCGTGGCGGCATAGAGACTTTTAAGCTTTCTAATCTTGATGGTTCCGCTGATAGTGAAAATATTGATAAATGGATTGCTTATCTATCAAGAGAAAGGATCAAAGAGTTCGTACTGGAACATACCAAATGGGATCCCTATAATGTCTTTTCCTGCATCTTTTTATATAAAGGTTTGACTCGTTTAGAGTTGCACAATTGTTTGTTGAATCCTCCGGCGACGTTCAAAGGCTTCCGGATGTTGAAGAGACTTCATCTTAAGCACGTTGATGTGTCCGGAGATGTGTTGGGAAGACTAATTGCTTGCTGTCCTCAGCTTGAGAGAGTTATTCTATGTGACTTGAAGGGTATAGCTGATGTTACTATTTGTGCACCAAGCATACAGTTCCTTAAAGTTCAAGGCAGTTTTGAGGATGTTGTACTAGAGAAGACCTTTAATCTTGTTGATGTTTCAATTGACTGGAAACAAGGTTCCACAATCTGTTCTGGCAAATTGCTCAGGCATATTTTGCAGCTGCCTCGTATTGAAAGACTCACACTGCAGCGTAGCTTTCTAGAGTGTGCTGTTGGTTCCTTGACCAAGAAGCTACCTAAATCATGTCTGTCTCTGAAGTTTCTTTCGATAAGCATACGCTTGGATAATTCGGAGGAGATTTTAACTGCCATGCACCTTATGAGAAGCGCACCTGCTCTAGAAGAACTAGACATTGTGTTTCGAAAAGGAAAGGTGACTAATGATGGAGAATCAACCTATTGTTTAAGTGATGACAAACAGAATTGGGAATTCACCCAACTGCAACGAGTGAAAGTAATCTGCTTCTCTGGTATCAAAGCAGAAGTAGACTTCATCAAATTTCTGTTATTAACCTCACCTGGTCTTCAAGAACTAGAAATTTCGTTTGGCCAAGATATGAAAGGAGTGGACTTTTCTTGGTTAGATGACAACCAGAAGAATTCTGCATTCACTCAAGTACGAGTTGTGAAAGTTACTCGCCTCTCTGGTGTCAAAGCCGAAGTAGATTTCATCAGATTTCTGCTCTCAAATACTTCACAAAGGCTTGAGAGAGTGATTCTACAACCTTCTTCTACCGAAGTTTCTTGGGAATTATTTAAGTTATTGCTGGCGTTCAAACGCGATACATTACATGCAGATTTTAAGTTCTTGGACCCATCAAGTCCTGTCACTCCTGTGCAGGATTCTGACGACTCGTCTTCTGACCACAACTTTGATGCTGCCTCATCTTCTGACTCCGACTTTGATGATGATGATGATGATGAATTGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box FBD LRR_2

Protein Analysis

537

Amino Acids

61.17

Weight (kDa)

6.48

Isoelectric Point (pI)

41.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	48 - 86	7.7e-06	F-box domain
LRR_At1g61320_AtMIF1	PF23622	109 - 410	6.8e-17	At1g61320/AtMIF1, LRR domain
LRR_At5g56370	PF24758	130 - 344	1.7e-18	FBD-associated F-box protein At5g56370, LRR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008865)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g09550 FvH4_5g09550 FvH4_5g09550 FvH4_5g09550 FvH4_5g10110 FvH4_5g10110 FvH4_5g10120 FvH4_5g10120 FvH4_6g18740
rosa_chinensis	RchiOBHm_Chr3g0473701
rosa_laevigata	RLG00000004674 RLG00000004675 RLG00000023990 RLG00000023992
rosa_multiflora	Rmu_co8411403.1_g000001 Rmu_sc0003069.1_g000001
rosa_roxburghii	Rroxscaffold_3G00266170 Rroxscaffold_6G00407920 Rroxscaffold_6G00407930
rosa_rugosa	Rorug03G0134800 Rorug06G0492000
rosa_samantha	Rh3BG213600 Rh7CG100200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1018
Acc36I	ACCTGC	1 cut(s)	1018
AccB7I	CCANNNNNTGG	1 cut(s)	34
AccI	GTMKAC	2 cut(s)	239, 1164
AccII	CGCG	1 cut(s)	1458
AccIII	TCCGGA	2 cut(s)	558, 599
AciI	CCGC	2 cut(s)	333, 372
AclWI	GGATC	5 cut(s)	78, 135, 431, 452, 465
AcoI	YGGCCR	1 cut(s)	1225
AcsI	RAATTY	4 cut(s)	1106, 1175, 1215, 1276
AcuI	CTGAAG	1 cut(s)	953
AfaI	GTAC	3 cut(s)	438, 744, 1296
AfiI	CCNNNNNNNGG	1 cut(s)	34
AflII	CTTAAG	1 cut(s)	581
AgeI	ACCGGT	1 cut(s)	158
AhdI	GACNNNNNGTC	1 cut(s)	1531
AjnI	CCWGG	2 cut(s)	198, 1195
AjuI	GAANNNNNNNTTGG	2 cut(s)	1206, 1238
AleI	CACNNNNGTG	1 cut(s)	1511
AloI	GAACNNNNNNTCC	2 cut(s)	778, 810
AluBI	AGCT	6 cut(s)	352, 640, 677, 838, 873, 910
AluI	AGCT	6 cut(s)	352, 640, 677, 838, 873, 910
Alw21I	GWGCWC	2 cut(s)	27, 697
Alw26I	GTCTC	4 cut(s)	252, 335, 565, 933
Alw44I	GTGCAC	1 cut(s)	693
AlwI	GGATC	5 cut(s)	78, 135, 431, 452, 465
Aor13HI	TCCGGA	2 cut(s)	558, 599
AoxI	GGCC	1 cut(s)	1225
ApaLI	GTGCAC	1 cut(s)	693
ApeKI	GCWGC	4 cut(s)	835, 838, 865, 1556
ApoI	RAATTY	4 cut(s)	1106, 1175, 1215, 1276
ArsI	GACNNNNNNTTYG	4 cut(s)	1532, 1562, 1564, 1594
AsiGI	ACCGGT	1 cut(s)	158
AspLEI	GCGC	1 cut(s)	1007
AspS9I	GGNCC	1 cut(s)	1489
AsuHPI	GGTGA	3 cut(s)	1063, 1102, 1185
AsuII	TTCGAA	1 cut(s)	1039
AvaII	GGWCC	1 cut(s)	1489
BaeGI	GKGCMC	1 cut(s)	697
BalI	TGGCCA	1 cut(s)	1227
BamHI	GGATCC	1 cut(s)	457
BbsI	GAAGAC	4 cut(s)	622, 758, 1193, 1527
Bbv12I	GWGCWC	2 cut(s)	27, 697
BbvCI	CCTCAGC	1 cut(s)	636
BbvI	GCAGC	4 cut(s)	825, 847, 877, 1543
BccI	CCATC	5 cut(s)	36, 118, 359, 1055, 1501
BcgI	CGANNNNNNTGC	2 cut(s)	824, 858
BciT130I	CCWGG	2 cut(s)	200, 1197
BclI	TGATCA	1 cut(s)	274
BcoDI	GTCTC	4 cut(s)	252, 335, 565, 933
BfaI	CTAG	6 cut(s)	225, 746, 878, 1016, 1025, 1211
BfmI	CTRYAG	1 cut(s)	863
BfrI	CTTAAG	1 cut(s)	581
BfuAI	ACCTGC	1 cut(s)	1018
BisI	GCNGC	5 cut(s)	334, 836, 839, 866, 1557
BlsI	GCNGC	5 cut(s)	335, 837, 840, 867, 1558
Bme1390I	CCNGG	2 cut(s)	200, 1197
Bme18I	GGWCC	1 cut(s)	1489
BmeRI	GACNNNNNGTC	1 cut(s)	1531
BmgT120I	GGNCC	1 cut(s)	1489
BmiI	GGNNCC	5 cut(s)	370, 459, 796, 895, 1491
BmrFI	CCNGG	2 cut(s)	200, 1197
BmsI	GCATC	2 cut(s)	489, 1543
BpiI	GAAGAC	4 cut(s)	622, 758, 1193, 1527
Bpu10I	CCTNAGC	2 cut(s)	636, 821
Bpu14I	TTCGAA	1 cut(s)	1039
BpuEI	CTTGAG	3 cut(s)	662, 1275, 1403
BsaI	GGTCTC	1 cut(s)	252
BsaWI	WCCGGW	3 cut(s)	158, 558, 599
Bsc4I	CCNNNNNNNGG	1 cut(s)	34
Bse118I	RCCGGY	1 cut(s)	158
Bse1I	ACTGG	2 cut(s)	444, 788
BseAI	TCCGGA	2 cut(s)	558, 599
BseBI	CCWGG	2 cut(s)	200, 1197
BseGI	GGATG	2 cut(s)	567, 742
BseLI	CCNNNNNNNGG	1 cut(s)	34
BseMII	CTCAG	3 cut(s)	94, 650, 835
BseNI	ACTGG	2 cut(s)	444, 788
BseRI	GAGGAG	1 cut(s)	986
BseSI	GKGCMC	1 cut(s)	697
BseXI	GCAGC	4 cut(s)	825, 847, 877, 1543
BsgI	GTGCAG	1 cut(s)	1535
Bsh1236I	CGCG	1 cut(s)	1458
BshFI	GGCC	1 cut(s)	1227
BshTI	ACCGGT	1 cut(s)	158
BsiHKAI	GWGCWC	2 cut(s)	27, 697
BsiSI	CCGG	4 cut(s)	159, 539, 559, 600
BslI	CCNNNNNNNGG	1 cut(s)	34
BsmAI	GTCTC	4 cut(s)	252, 335, 565, 933
BsmI	GAATGC	2 cut(s)	59, 1283
BsnI	GGCC	1 cut(s)	1227
Bso31I	GGTCTC	1 cut(s)	252
Bsp119I	TTCGAA	1 cut(s)	1039
Bsp1286I	GDGCHC	2 cut(s)	27, 697
Bsp13I	TCCGGA	2 cut(s)	558, 599
Bsp143I	GATC	6 cut(s)	18, 83, 127, 274, 423, 457
BspACI	CCGC	2 cut(s)	333, 372
BspANI	GGCC	1 cut(s)	1227
BspCNI	CTCAG	3 cut(s)	93, 649, 834
BspEI	TCCGGA	2 cut(s)	558, 599
BspFNI	CGCG	1 cut(s)	1458
BspLI	GGNNCC	5 cut(s)	370, 459, 796, 895, 1491
BspMAI	CTGCAG	1 cut(s)	867
BspMI	ACCTGC	1 cut(s)	1018
BspPI	GGATC	5 cut(s)	78, 135, 431, 452, 465
BspT104I	TTCGAA	1 cut(s)	1039
BspTI	CTTAAG	1 cut(s)	581
BspTNI	GGTCTC	1 cut(s)	252
BsrFI	RCCGGY	1 cut(s)	158
BsrI	ACTGG	2 cut(s)	444, 788
BssAI	RCCGGY	1 cut(s)	158
BssMI	GATC	6 cut(s)	18, 83, 127, 274, 423, 457
Bst2UI	CCWGG	2 cut(s)	200, 1197
Bst4CI	ACNGT	1 cut(s)	708
Bst6I	CTCTTC	1 cut(s)	563
BstAFI	CTTAAG	1 cut(s)	581
BstAPI	GCANNNNNTGC	1 cut(s)	832
BstBI	TTCGAA	1 cut(s)	1039
BstC8I	GCNNGC	2 cut(s)	628, 1446
BstDEI	CTNAG	4 cut(s)	68, 80, 636, 821
BstF5I	GGATG	2 cut(s)	567, 742
BstFNI	CGCG	1 cut(s)	1458
BstHHI	GCGC	1 cut(s)	1007
BstKTI	GATC	6 cut(s)	21, 86, 130, 277, 426, 460
BstMAI	GTCTC	4 cut(s)	252, 335, 565, 933
BstMBI	GATC	6 cut(s)	18, 83, 127, 274, 423, 457
BstMWI	GCNNNNNNNGC	1 cut(s)	832
BstNI	CCWGG	2 cut(s)	200, 1197
BstNSI	RCATGY	1 cut(s)	1472
BstSCI	CCNGG	2 cut(s)	198, 1195
BstSFI	CTRYAG	1 cut(s)	863
BstSLI	GKGCMC	1 cut(s)	697
BstUI	CGCG	1 cut(s)	1458
BstV1I	GCAGC	4 cut(s)	825, 847, 877, 1543
BstV2I	GAAGAC	4 cut(s)	622, 758, 1193, 1527
BstX2I	RGATCY	2 cut(s)	83, 457
BstYI	RGATCY	2 cut(s)	83, 457
BsuRI	GGCC	1 cut(s)	1227
BtsCI	GGATG	2 cut(s)	567, 742
BtsI	GCAGTG	2 cut(s)	210, 860
BtsIMutI	CAGTG	2 cut(s)	210, 860
BveI	ACCTGC	1 cut(s)	1018
Cac8I	GCNNGC	2 cut(s)	628, 1446
CfoI	GCGC	1 cut(s)	1007
Cfr10I	RCCGGY	1 cut(s)	158
Cfr13I	GGNCC	1 cut(s)	1489
CsiI	ACCWGGT	1 cut(s)	1195
Csp6I	GTAC	3 cut(s)	437, 743, 1295
CspAI	ACCGGT	1 cut(s)	158
CviAII	CATG	5 cut(s)	243, 311, 921, 988, 1469
CviQI	GTAC	3 cut(s)	437, 743, 1295
DdeI	CTNAG	4 cut(s)	68, 80, 636, 821
DpnI	GATC	6 cut(s)	20, 85, 129, 276, 425, 459
DpnII	GATC	6 cut(s)	18, 83, 127, 274, 423, 457
DriI	GACNNNNNGTC	1 cut(s)	1531
EaeI	YGGCCR	1 cut(s)	1225
Eam1104I	CTCTTC	1 cut(s)	563
Eam1105I	GACNNNNNGTC	1 cut(s)	1531
EarI	CTCTTC	1 cut(s)	563
Eco31I	GGTCTC	1 cut(s)	252
Eco32I	GATATC	1 cut(s)	292
Eco47I	GGWCC	1 cut(s)	1489
Eco57I	CTGAAG	1 cut(s)	953
EcoRI	GAATTC	2 cut(s)	1106, 1276
EcoRII	CCWGG	2 cut(s)	198, 1195
EcoRV	GATATC	1 cut(s)	292
FaeI	CATG	5 cut(s)	246, 314, 924, 991, 1472
FatI	CATG	5 cut(s)	242, 310, 920, 987, 1468
FbaI	TGATCA	1 cut(s)	274
FblI	GTMKAC	2 cut(s)	239, 1164
Fnu4HI	GCNGC	5 cut(s)	334, 836, 839, 866, 1557
FokI	GGATG	2 cut(s)	574, 749
Fsp4HI	GCNGC	5 cut(s)	334, 836, 839, 866, 1557
FspBI	CTAG	6 cut(s)	225, 746, 878, 1016, 1025, 1211
GlaI	GCGC	1 cut(s)	1006
GluI	GCNGC	5 cut(s)	334, 836, 839, 866, 1557
HaeIII	GGCC	1 cut(s)	1227
HapII	CCGG	4 cut(s)	159, 539, 559, 600
HhaI	GCGC	1 cut(s)	1007
Hin1II	CATG	5 cut(s)	246, 314, 924, 991, 1472
Hin6I	GCGC	1 cut(s)	1005
HinP1I	GCGC	1 cut(s)	1005
HincII	GTYRAC	1 cut(s)	240
HindII	GTYRAC	1 cut(s)	240
HindIII	AAGCTT	1 cut(s)	350
HinfI	GANTC	8 cut(s)	502, 532, 855, 1067, 1393, 1520, 1529, 1571
HpaII	CCGG	4 cut(s)	159, 539, 559, 600
HphI	GGTGA	3 cut(s)	1063, 1102, 1185
Hpy166II	GTNNAC	4 cut(s)	240, 695, 1165, 1246
Hpy188III	TCNNGA	7 cut(s)	362, 414, 559, 600, 878, 1016, 1205
Hpy8I	GTNNAC	4 cut(s)	240, 695, 1165, 1246
Hpy99I	CGWCG	1 cut(s)	547
HpyAV	CCTTC	2 cut(s)	661, 1413
HpyCH4III	ACNGT	1 cut(s)	708
HpyCH4IV	ACGT	2 cut(s)	545, 588
HpyF10VI	GCNNNNNNNGC	1 cut(s)	832
HpyF3I	CTNAG	4 cut(s)	68, 80, 636, 821
HpySE526I	ACGT	2 cut(s)	545, 588
Hsp92II	CATG	5 cut(s)	246, 314, 924, 991, 1472
HspAI	GCGC	1 cut(s)	1005
Kpn2I	TCCGGA	2 cut(s)	558, 599
Ksp22I	TGATCA	1 cut(s)	274
Kzo9I	GATC	6 cut(s)	18, 83, 127, 274, 423, 457
LmnI	GCTCC	1 cut(s)	30
Lsp1109I	GCAGC	4 cut(s)	825, 847, 877, 1543
LweI	GCATC	2 cut(s)	489, 1543
MabI	ACCWGGT	1 cut(s)	1195
MaeI	CTAG	6 cut(s)	225, 746, 878, 1016, 1025, 1211
MaeII	ACGT	2 cut(s)	545, 588
MaeIII	GTNAC	7 cut(s)	163, 318, 659, 682, 1051, 1309, 1504
MalI	GATC	6 cut(s)	20, 85, 129, 276, 425, 459
MboI	GATC	6 cut(s)	18, 83, 127, 274, 423, 457
MfeI	CAATTG	2 cut(s)	520, 777
MflI	RGATCY	2 cut(s)	83, 457
MhlI	GDGCHC	2 cut(s)	27, 697
MlsI	TGGCCA	1 cut(s)	1227
MluNI	TGGCCA	1 cut(s)	1227
MlyI	GAGTC	4 cut(s)	496, 849, 1523, 1565
MmeI	TCCRAC	2 cut(s)	114, 1599
Mox20I	TGGCCA	1 cut(s)	1227
MroI	TCCGGA	2 cut(s)	558, 599
MscI	TGGCCA	1 cut(s)	1227
MslI	CAYNNNNRTG	2 cut(s)	591, 1511
Msp20I	TGGCCA	1 cut(s)	1227
MspA1I	CMGCKG	2 cut(s)	374, 838
MspCI	CTTAAG	1 cut(s)	581
MspI	CCGG	4 cut(s)	159, 539, 559, 600
MspR9I	CCNGG	2 cut(s)	200, 1197
MunI	CAATTG	2 cut(s)	520, 777
Mva1269I	GAATGC	2 cut(s)	59, 1283
MvaI	CCWGG	2 cut(s)	200, 1197
MvnI	CGCG	1 cut(s)	1458
MwoI	GCNNNNNNNGC	1 cut(s)	832
NdeII	GATC	6 cut(s)	18, 83, 127, 274, 423, 457
NlaIII	CATG	5 cut(s)	246, 314, 924, 991, 1472
NlaIV	GGNNCC	5 cut(s)	370, 459, 796, 895, 1491
NmeAIII	GCCGAG	1 cut(s)	229
NmuCI	GTSAC	3 cut(s)	659, 1051, 1504
NspI	RCATGY	1 cut(s)	1472
NspV	TTCGAA	1 cut(s)	1039
OliI	CACNNNNGTG	1 cut(s)	1511
PaqCI	CACCTGC	1 cut(s)	1018
PctI	GAATGC	2 cut(s)	59, 1283
PfeI	GAWTC	4 cut(s)	532, 1067, 1393, 1520
PflFI	GACNNNGTC	1 cut(s)	244
PflMI	CCANNNNNTGG	1 cut(s)	34
PinAI	ACCGGT	1 cut(s)	158
PkrI	GCNGC	5 cut(s)	335, 837, 840, 867, 1558
PleI	GAGTC	4 cut(s)	496, 849, 1523, 1565
PpsI	GAGTC	4 cut(s)	496, 849, 1523, 1565
Psp6I	CCWGG	2 cut(s)	198, 1195
PspGI	CCWGG	2 cut(s)	198, 1195
PspN4I	GGNNCC	5 cut(s)	370, 459, 796, 895, 1491
PspPI	GGNCC	1 cut(s)	1489
PstI	CTGCAG	1 cut(s)	867
PsuI	RGATCY	2 cut(s)	83, 457
PsyI	GACNNNGTC	1 cut(s)	244
PvuII	CAGCTG	1 cut(s)	838
RsaI	GTAC	3 cut(s)	438, 744, 1296
RsaNI	GTAC	3 cut(s)	437, 743, 1295
RseI	CAYNNNNRTG	2 cut(s)	591, 1511
SalI	GTCGAC	1 cut(s)	238
SatI	GCNGC	5 cut(s)	334, 836, 839, 866, 1557
Sau3AI	GATC	6 cut(s)	18, 83, 127, 274, 423, 457
Sau96I	GGNCC	1 cut(s)	1489
SchI	GAGTC	4 cut(s)	496, 849, 1523, 1565
ScrFI	CCNGG	2 cut(s)	200, 1197
SduI	GDGCHC	2 cut(s)	27, 697
SexAI	ACCWGGT	1 cut(s)	1195
SfaNI	GCATC	2 cut(s)	489, 1543
SfcI	CTRYAG	1 cut(s)	863
SfuI	TTCGAA	1 cut(s)	1039
SinI	GGWCC	1 cut(s)	1489
SmiMI	CAYNNNNRTG	2 cut(s)	591, 1511
SmlI	CTYRAG	4 cut(s)	581, 641, 1290, 1382
SmoI	CTYRAG	4 cut(s)	581, 641, 1290, 1382
SsiI	CCGC	2 cut(s)	333, 372
SspI	AATATT	2 cut(s)	15, 388
SspMI	CTAG	6 cut(s)	225, 746, 878, 1016, 1025, 1211
StyD4I	CCNGG	2 cut(s)	198, 1195
TaaI	ACNGT	1 cut(s)	708
TaiI	ACGT	2 cut(s)	548, 591
TaqI	TCGA	5 cut(s)	73, 195, 239, 944, 1039
TatI	WGTACW	1 cut(s)	742
TauI	GCSGC	1 cut(s)	336
TfiI	GAWTC	4 cut(s)	532, 1067, 1393, 1520
TscAI	CASTG	2 cut(s)	210, 867
TseFI	GTSAC	3 cut(s)	659, 1051, 1504
TseI	GCWGC	4 cut(s)	835, 838, 865, 1556
Tsp45I	GTSAC	3 cut(s)	659, 1051, 1504
TspDTI	ATGAA	5 cut(s)	314, 566, 1159, 1250, 1336
TspRI	CASTG	2 cut(s)	210, 867
Tth111I	GACNNNGTC	1 cut(s)	244
Van91I	CCANNNNNTGG	1 cut(s)	34
Vha464I	CTTAAG	1 cut(s)	581
VneI	GTGCAC	1 cut(s)	693
VpaK11BI	GGWCC	1 cut(s)	1489
XapI	RAATTY	4 cut(s)	1106, 1175, 1215, 1276
XbaI	TCTAGA	2 cut(s)	877, 1015
XceI	RCATGY	1 cut(s)	1472
XmiI	GTMKAC	2 cut(s)	239, 1164
XspI	CTAG	6 cut(s)	225, 746, 878, 1016, 1025, 1211

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.