RchiOBHm_Chr3g0473701

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0008865
Physical Location & Seq
Reverse (-)
19474327 .. 19476183
1857 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43936

Sequence Viewer

Length: 1476 bp
ATGTCAGATCCTAAGAAAACCATCCAATCCAATTCCTGTTTGATAAAGAAGAAGAAGATGGATCAGTTGGACAAATTAAGCAACCTACCAACTGGTGTTACACACCAGATTTTGTCATACTTACCTATCAAGCAGGCAGTGAGGACAAGTATTTTATCAAGTGACTGGAGGTACAAATGGGCTCAGCTTCCATGTCTTGTGTTTGATGATAGAACTCATAGAAGTGGATATCCAATTGCAAAGGTTGTGGATCATGTGCTGTTACTTCATAGTGGCCGCATTGACACTTTCAAGCTTTCTACTTTTAACTATCCAGCCGCTAGGGATATAGATAAATGGATTTCTCATCTATCAAGAAGCAATGTCAAAGTGTTCGTACTGAAAGTATGGGAGCTGCTGCCTCGATACAATGTGTTTTCCTGCTTCTTTTCTTTTAAAGATTTGACTCATTTAGAGTTATGCAATTGTTTGCTTAAACCTCCAACGACATTCAAAGGCTTCAGGATGTTGAAGAGCCTTCATCTTCAAGAAGTTATTGTGTCCGGACATGTGTTGGGAAAACTAATAGCTTGCTGTCCTCAGCTTGAGAGAATAATTCTATGTGACTTAAAGGGAATCACTGATGTCACTATTGATGCACCAAGTATCCGGTTCCTTAGAGTTCGAGGCAGTTTTGAGGATGTTCTACTAGCAAATACCTTAAATCTTGTTGATGTTTCAATTGACTGGAAACAAGTTTCTACAATCAGTTCTAGCAAATTGCTCAGGTATTTTTTTGAACTGCCTCGTATTGAAAGGCTCACACTTAAGGATGGCTTTTTAGAGTATGCTGTTGGTTCCTTGACCAAGAATCTGCTGCCTACACCATGTCTATGTCTGAAGTTTCTTTCTATAAGAATATGCTTGAATAATTTGGATGAGATTTCAACTGCTATCCACCTTATGAGAAGCGCACCTGCTCTACAAGAACTAGACATTTTGGTTCGAAAAGGAGAGGAGACTAATGATGGAAAAGCAACCTATTGTTTAATTGACAAACAGAATTGGGAATTCACCCAACTGCAACGAATGAAAGTAACCGGCTTCTCTGGTGTCAAAGCAGAAGTAGACTTCATTGAATTTCTGTTATTAACCTCACCTGGTCTTCAAGAACTAGAAATTTCATTTTGTCAAGATGTGAAAAAAGTGGACTTTTCTTGGTTAGATGACAACCAGAAGACTTGTGCATTCACACAACTGCTAGTTGTGAAAGTAACTGGCTTCTCTGGTGTCAGAGCCGAAGTAGATTTCATTAGATTTCTGCTTTCAAGCACTTCTAAAGTGATTCTTCAACCTGCTTCTGTCAAAGTTTATTGGGATGTATTTAAATTATTGCCGGTGTTCAGACGCGACTCATTACATGCAGATTTAAAGCTCTTGGGCCCATCAATTCATGTACAGGATTCTGATGACTCATCCTTCTGTCTCCGACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

491

Amino Acids

56.03

Weight (kDa)

8.89

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 89 - 209 5.8e-09 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 110 - 326 1.5e-15 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1 PF23622 208 - 408 9.2e-07 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 964
Acc36I ACCTGC 2 cut(s) 964, 1342
AccI GTMKAC 1 cut(s) 1107
AccII CGCG 1 cut(s) 1389
AccIII TCCGGA 1 cut(s) 542
AciI CCGC 2 cut(s) 277, 318
AclWI GGATC 3 cut(s) 2, 69, 258
AcoI YGGCCR 1 cut(s) 274
AcsI RAATTY 3 cut(s) 1049, 1118, 1158
AcuI CTGAAG 2 cut(s) 484, 899
AfaI GTAC 3 cut(s) 173, 378, 1437
AflII CTTAAG 1 cut(s) 806
AflIII ACRYGT 1 cut(s) 547
AjnI CCWGG 1 cut(s) 1138
AluBI AGCT 6 cut(s) 187, 295, 394, 569, 583, 1414
AluI AGCT 6 cut(s) 187, 295, 394, 569, 583, 1414
Alw26I GTCTC 2 cut(s) 992, 1469
AlwI GGATC 3 cut(s) 2, 69, 258
Aor13HI TCCGGA 1 cut(s) 542
AoxI GGCC 2 cut(s) 274, 1420
ApaI GGGCCC 1 cut(s) 1424
ApeKI GCWGC 3 cut(s) 394, 397, 856
ApoI RAATTY 3 cut(s) 1049, 1118, 1158
AspLEI GCGC 1 cut(s) 953
AspS9I GGNCC 2 cut(s) 1420, 1421
AsuHPI GGTGA 2 cut(s) 1045, 1128
AsuII TTCGAA 1 cut(s) 985
BaeGI GKGCMC 1 cut(s) 1424
BanII GRGCYC 2 cut(s) 184, 1424
BbsI GAAGAC 2 cut(s) 1136, 1223
BbvCI CCTCAGC 1 cut(s) 579
BbvI GCAGC 3 cut(s) 381, 384, 843
BccI CCATC 5 cut(s) 29, 52, 806, 1001, 1432
BciT130I CCWGG 1 cut(s) 1140
BciVI GTATCC 1 cut(s) 656
BcoDI GTCTC 2 cut(s) 992, 1469
BfaI CTAG 6 cut(s) 321, 689, 753, 971, 1154, 1241
BfrI CTTAAG 1 cut(s) 806
BfuAI ACCTGC 2 cut(s) 964, 1342
BfuI GTATCC 1 cut(s) 656
BisI GCNGC 5 cut(s) 277, 318, 395, 398, 857
BlpI GCTNAGC 1 cut(s) 183
BlsI GCNGC 5 cut(s) 278, 319, 396, 399, 858
Bme1390I CCNGG 1 cut(s) 1140
BmgT120I GGNCC 2 cut(s) 1420, 1421
BmiI GGNNCC 3 cut(s) 653, 838, 1422
BmrFI CCNGG 1 cut(s) 1140
BmsI GCATC 1 cut(s) 625
BpiI GAAGAC 2 cut(s) 1136, 1223
BpmI CTGGAG 1 cut(s) 187
Bpu10I CCTNAGC 2 cut(s) 579, 764
Bpu1102I GCTNAGC 1 cut(s) 183
Bpu14I TTCGAA 1 cut(s) 985
BpuEI CTTGAG 1 cut(s) 605
BsaWI WCCGGW 2 cut(s) 542, 648
Bse118I RCCGGY 2 cut(s) 1079, 1375
Bse1I ACTGG 4 cut(s) 97, 170, 731, 1261
Bse3DI GCAATG 1 cut(s) 367
BseAI TCCGGA 1 cut(s) 542
BseBI CCWGG 1 cut(s) 1140
BseGI GGATG 7 cut(s) 21, 510, 685, 817, 922, 1363, 1454
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 3 cut(s) 197, 593, 778
BseNI ACTGG 4 cut(s) 97, 170, 731, 1261
BseRI GAGGAG 1 cut(s) 1010
BseSI GKGCMC 1 cut(s) 1424
BseXI GCAGC 3 cut(s) 381, 384, 843
Bsh1236I CGCG 1 cut(s) 1389
BshFI GGCC 2 cut(s) 276, 1422
BsiSI CCGG 4 cut(s) 543, 649, 1080, 1376
BsmAI GTCTC 2 cut(s) 992, 1469
BsmI GAATGC 1 cut(s) 1226
BsnI GGCC 2 cut(s) 276, 1422
Bsp119I TTCGAA 1 cut(s) 985
Bsp120I GGGCCC 1 cut(s) 1420
Bsp1286I GDGCHC 2 cut(s) 184, 1424
Bsp13I TCCGGA 1 cut(s) 542
Bsp1407I TGTACA 1 cut(s) 1435
Bsp143I GATC 3 cut(s) 7, 61, 250
Bsp1720I GCTNAGC 1 cut(s) 183
BspACI CCGC 2 cut(s) 277, 318
BspANI GGCC 2 cut(s) 276, 1422
BspCNI CTCAG 3 cut(s) 196, 592, 777
BspEI TCCGGA 1 cut(s) 542
BspFNI CGCG 1 cut(s) 1389
BspLI GGNNCC 3 cut(s) 653, 838, 1422
BspMI ACCTGC 2 cut(s) 964, 1342
BspPI GGATC 3 cut(s) 2, 69, 258
BspQI GCTCTTC 1 cut(s) 506
BspT104I TTCGAA 1 cut(s) 985
BspTI CTTAAG 1 cut(s) 806
BsrDI GCAATG 1 cut(s) 367
BsrFI RCCGGY 2 cut(s) 1079, 1375
BsrGI TGTACA 1 cut(s) 1435
BsrI ACTGG 4 cut(s) 97, 170, 731, 1261
BssAI RCCGGY 2 cut(s) 1079, 1375
BssMI GATC 3 cut(s) 7, 61, 250
Bst2UI CCWGG 1 cut(s) 1140
Bst6I CTCTTC 1 cut(s) 506
BstAFI CTTAAG 1 cut(s) 806
BstAUI TGTACA 1 cut(s) 1435
BstBI TTCGAA 1 cut(s) 985
BstC8I GCNNGC 2 cut(s) 135, 571
BstDEI CTNAG 5 cut(s) 12, 183, 579, 656, 764
BstF5I GGATG 7 cut(s) 21, 510, 685, 817, 922, 1363, 1454
BstFNI CGCG 1 cut(s) 1389
BstHHI GCGC 1 cut(s) 953
BstKTI GATC 3 cut(s) 10, 64, 253
BstMAI GTCTC 2 cut(s) 992, 1469
BstMBI GATC 3 cut(s) 7, 61, 250
BstNI CCWGG 1 cut(s) 1140
BstNSI RCATGY 2 cut(s) 551, 1403
BstSCI CCNGG 1 cut(s) 1138
BstSLI GKGCMC 1 cut(s) 1424
BstUI CGCG 1 cut(s) 1389
BstV1I GCAGC 3 cut(s) 381, 384, 843
BstV2I GAAGAC 2 cut(s) 1136, 1223
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuI GTATCC 1 cut(s) 656
BsuRI GGCC 2 cut(s) 276, 1422
BtsCI GGATG 7 cut(s) 21, 510, 685, 817, 922, 1363, 1454
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 2 cut(s) 144, 618
BveI ACCTGC 2 cut(s) 964, 1342
Cac8I GCNNGC 2 cut(s) 135, 571
CfoI GCGC 1 cut(s) 953
Cfr10I RCCGGY 2 cut(s) 1079, 1375
Cfr13I GGNCC 2 cut(s) 1420, 1421
CseI GACGC 1 cut(s) 1395
CsiI ACCWGGT 1 cut(s) 1138
Csp6I GTAC 3 cut(s) 172, 377, 1436
CspCI CAANNNNNGTGG 2 cut(s) 228, 263
CviAII CATG 6 cut(s) 192, 254, 548, 867, 1400, 1433
CviQI GTAC 3 cut(s) 172, 377, 1436
DdeI CTNAG 5 cut(s) 12, 183, 579, 656, 764
DpnI GATC 3 cut(s) 9, 63, 252
DpnII GATC 3 cut(s) 7, 61, 250
DraI TTTAAA 3 cut(s) 436, 1366, 1410
EaeI YGGCCR 1 cut(s) 274
Eam1104I CTCTTC 1 cut(s) 506
EarI CTCTTC 1 cut(s) 506
Eco24I GRGCYC 2 cut(s) 184, 1424
Eco32I GATATC 1 cut(s) 230
Eco57I CTGAAG 2 cut(s) 484, 899
EcoRI GAATTC 1 cut(s) 1049
EcoRII CCWGG 1 cut(s) 1138
EcoRV GATATC 1 cut(s) 230
EcoT38I GRGCYC 2 cut(s) 184, 1424
FaeI CATG 6 cut(s) 195, 257, 551, 870, 1403, 1436
FalI AAGNNNNNCTT 4 cut(s) 800, 832, 1311, 1343
FatI CATG 6 cut(s) 191, 253, 547, 866, 1399, 1432
FblI GTMKAC 1 cut(s) 1107
Fnu4HI GCNGC 5 cut(s) 277, 318, 395, 398, 857
FokI GGATG 7 cut(s) 8, 517, 692, 824, 929, 1370, 1441
FriOI GRGCYC 2 cut(s) 184, 1424
Fsp4HI GCNGC 5 cut(s) 277, 318, 395, 398, 857
FspBI CTAG 6 cut(s) 321, 689, 753, 971, 1154, 1241
GlaI GCGC 1 cut(s) 952
GluI GCNGC 5 cut(s) 277, 318, 395, 398, 857
GsuI CTGGAG 1 cut(s) 187
HaeIII GGCC 2 cut(s) 276, 1422
HapII CCGG 4 cut(s) 543, 649, 1080, 1376
HgaI GACGC 1 cut(s) 1395
HhaI GCGC 1 cut(s) 953
Hin1II CATG 6 cut(s) 195, 257, 551, 870, 1403, 1436
Hin6I GCGC 1 cut(s) 951
HinP1I GCGC 1 cut(s) 951
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 8 cut(s) 445, 615, 850, 1324, 1391, 1442, 1451, 1470
HpaII CCGG 4 cut(s) 543, 649, 1080, 1376
HphI GGTGA 2 cut(s) 1045, 1128
Hpy166II GTNNAC 2 cut(s) 1108, 1189
Hpy188I TCNGA 7 cut(s) 7, 879, 1274, 1385, 1447, 1469, 1475
Hpy188III TCNNGA 6 cut(s) 354, 502, 527, 543, 1148, 1172
Hpy8I GTNNAC 2 cut(s) 1108, 1189
HpyAV CCTTC 2 cut(s) 527, 1468
HpyCH4V TGCA 6 cut(s) 239, 462, 638, 1063, 1226, 1403
HpyF3I CTNAG 5 cut(s) 12, 183, 579, 656, 764
Hsp92II CATG 6 cut(s) 195, 257, 551, 870, 1403, 1436
HspAI GCGC 1 cut(s) 951
Kpn2I TCCGGA 1 cut(s) 542
Kzo9I GATC 3 cut(s) 7, 61, 250
LguI GCTCTTC 1 cut(s) 506
LmnI GCTCC 1 cut(s) 391
Lsp1109I GCAGC 3 cut(s) 381, 384, 843
LweI GCATC 1 cut(s) 625
MabI ACCWGGT 1 cut(s) 1138
MaeI CTAG 6 cut(s) 321, 689, 753, 971, 1154, 1241
MaeIII GTNAC 7 cut(s) 97, 161, 261, 602, 625, 1075, 1252
MalI GATC 3 cut(s) 9, 63, 252
MboI GATC 3 cut(s) 7, 61, 250
MboII GAAGA 8 cut(s) 61, 64, 67, 515, 523, 1136, 1228, 1319
MfeI CAATTG 3 cut(s) 234, 463, 720
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 184, 1424
MlyI GAGTC 4 cut(s) 439, 1385, 1445, 1464
MmeI TCCRAC 2 cut(s) 48, 506
MroI TCCGGA 1 cut(s) 542
MslI CAYNNNNRTG 2 cut(s) 222, 871
MspCI CTTAAG 1 cut(s) 806
MspI CCGG 4 cut(s) 543, 649, 1080, 1376
MspR9I CCNGG 1 cut(s) 1140
MunI CAATTG 3 cut(s) 234, 463, 720
Mva1269I GAATGC 1 cut(s) 1226
MvaI CCWGG 1 cut(s) 1140
MvnI CGCG 1 cut(s) 1389
NdeII GATC 3 cut(s) 7, 61, 250
NlaIII CATG 6 cut(s) 195, 257, 551, 870, 1403, 1436
NlaIV GGNNCC 3 cut(s) 653, 838, 1422
NmuCI GTSAC 3 cut(s) 161, 602, 625
NspI RCATGY 2 cut(s) 551, 1403
NspV TTCGAA 1 cut(s) 985
PaqCI CACCTGC 1 cut(s) 964
PciI ACATGT 1 cut(s) 547
PciSI GCTCTTC 1 cut(s) 506
PctI GAATGC 1 cut(s) 1226
PfeI GAWTC 4 cut(s) 615, 850, 1324, 1442
PkrI GCNGC 5 cut(s) 278, 319, 396, 399, 858
PleI GAGTC 4 cut(s) 439, 1385, 1445, 1464
PpsI GAGTC 4 cut(s) 439, 1385, 1445, 1464
PscI ACATGT 1 cut(s) 547
Psp6I CCWGG 1 cut(s) 1138
PspGI CCWGG 1 cut(s) 1138
PspN4I GGNNCC 3 cut(s) 653, 838, 1422
PspOMI GGGCCC 1 cut(s) 1420
PspPI GGNCC 2 cut(s) 1420, 1421
PsuI RGATCY 1 cut(s) 7
RsaI GTAC 3 cut(s) 173, 378, 1437
RsaNI GTAC 3 cut(s) 172, 377, 1436
RseI CAYNNNNRTG 2 cut(s) 222, 871
SapI GCTCTTC 1 cut(s) 506
SatI GCNGC 5 cut(s) 277, 318, 395, 398, 857
Sau3AI GATC 3 cut(s) 7, 61, 250
Sau96I GGNCC 2 cut(s) 1420, 1421
SchI GAGTC 4 cut(s) 439, 1385, 1445, 1464
ScrFI CCNGG 1 cut(s) 1140
SduI GDGCHC 2 cut(s) 184, 1424
SexAI ACCWGGT 1 cut(s) 1138
SfaNI GCATC 1 cut(s) 625
SfuI TTCGAA 1 cut(s) 985
SmiI ATTTAAAT 1 cut(s) 1366
SmiMI CAYNNNNRTG 2 cut(s) 222, 871
SmlI CTYRAG 2 cut(s) 584, 806
SmoI CTYRAG 2 cut(s) 584, 806
SsiI CCGC 2 cut(s) 277, 318
SspMI CTAG 6 cut(s) 321, 689, 753, 971, 1154, 1241
StyD4I CCNGG 1 cut(s) 1138
SwaI ATTTAAAT 1 cut(s) 1366
TaqI TCGA 3 cut(s) 403, 664, 985
TatI WGTACW 1 cut(s) 1435
TauI GCSGC 2 cut(s) 279, 320
TfiI GAWTC 4 cut(s) 615, 850, 1324, 1442
TscAI CASTG 2 cut(s) 144, 625
TseFI GTSAC 3 cut(s) 161, 602, 625
TseI GCWGC 3 cut(s) 394, 397, 856
Tsp45I GTSAC 3 cut(s) 161, 602, 625
TspDTI ATGAA 7 cut(s) 257, 509, 1085, 1102, 1152, 1279, 1421
TspRI CASTG 2 cut(s) 144, 625
Vha464I CTTAAG 1 cut(s) 806
XapI RAATTY 3 cut(s) 1049, 1118, 1158
XceI RCATGY 2 cut(s) 551, 1403
XmiI GTMKAC 1 cut(s) 1107
XspI CTAG 6 cut(s) 321, 689, 753, 971, 1154, 1241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.