FvH4_6g36300

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001657
Physical Location & Seq
Forward (+)
28542410 .. 28570735
28326 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g36300.t4

Sequence Viewer

Length: 10266 bp
ATGTTCGAACGGCTCGTCACTCAGGTCCTTCACGGCTACCTAGGTCGCTACGTCAAGGATTTTCACAAGCACCAGCTCAAGATCACCATCTGGAACGAGGAAGTGTTCTTGGAGAACGTGGAGCTTAGTCTCGAAGCCTTTGATTATCTCCAGTTGCCTTTCGCTCTCAAACAAGGTCGGATTGGAAAAATAAGCATCAAAATTCCATGGAAAAAGCTTGGTTGGGAGCCCTTTGTAATTAGTTTGGAAAATGTCTTCCTATGTGCATCCCAGAGAGATGATGAAGAGTGGAGTTTGGATGAAGTTGAAAAACGAGAGTTTGCTGGAAAAAAGGCCAAACTTGCCGCTGCAGAGCTGGCCAAATTATCAAAACGTGTATGCGAAAATCAAGCTGGGTTTATATCAATAATTACTGTGAAGATTATTGACAGCATCCAAGTGTCAATCAGAGATTTTCATATCTTGTACCATGACAAGCTGAGTGGCTCGGTATGTAATATTTTTGGTTTGAAGTTTTCCAGCTTGAGGACTATGAAGCAAAATCCTTCCTGGTCAGTTGCAAAGGGAAGAGGGGCCCAAGTTAACAAAACTGTGGAGATTATGGGTTTGGAGTTTTATTGTGGCACACATGATGGGCCGGTGGAGTTGATGAATATGAACAATAGCGGGGACTCCACAGTATGGCAAGATACAAGATATGATGAGAAAAGATATAACAACTCTATACTGTCACCATGTGATGTTTCCATGTCACTTTTGGTCAACAGATCAGGAGAGCTTGGTAGCAAAATTCCGCAGTACTCCGTCACCGCTGAGATAACTGACTTGGTGATGTCCATAGATGAGGTTCAGTTGCAACAGATATTGTTTCTTTGGGATTATTTCTGTACATGTGAATTAAGAAATACGTATGGGCGTTACCGTCCCTGGAGTAGTCCGTTATCAAAAAAGGTGAAGGGTTGGCAAATGTTGTGGTGGCATTATGCGCAAGAATCTATCTTATCAGATGTGCGGAAGAGATTAAAGAAAACATCGTGGAGACACTTTGGACAGAGATTAAGCTCTTGTCGGAAGTATGTTAACCTTTATAAAACTAAGCTGGATTTTCTTCGCCATGATCAGCCAATTGATGAAAGTACTCGATGGGAATTGGAGCAGATGGAGAAAGAATTAGACATAGATGATATTTTAAGTTACAGGTCCGCTGCAGAATGTGAGTTGCAGGAGATGTTGGTGAACACTTCAACTGGAAAATCCCGTGGATGGCTGAACTGGCTATCTCTTGGTATGCTTGGTGCTGGAGGAACAGAGAATACGGATCAATTTTCTGGGGCTGTTTCTCTTTCTGATGCAGCTATTAAGGATATATATGAGGCAACCGAGTTCAATCCTCCTATTTTATCCAATGGGGTTGCTCCAACAAATGATGAGATTGATATATGTGCACTCCAGTTCAACATTAATCGGATTTCTGCAACACTACGTAACATGAAGTATGGCCAAGAAATTGCAGAGTTGATGTTGAATGGGGTGACTGTAGAGTGCAAATTTAGGGAAGAATCTGCAGCCATTGTCGCCATTGTGAAGTCAGGGGAGATGGTTTATCCTTGTAACAAGAAAATCATTCTGCATTTGCAAGGGCCTAATAATGAGACGAACGAAGTGGAAAACGAGAATCCTTCCTTTAGATTGCAGGTGGATGTATCATCTGATCTCGAGGCTGAATTGTCCATTAAGGGGATGCTTCAACCATTAGAAGTCACCATTGATGCAGAATTTTTCTTAAAGTTGATGGATTTTCTTGGAGCGTTAAAAACCATTGAAAGTCAGCATGGAAGGGTCCTTATGTCACTAAATGGGATAGAAAATGTCAATGGTCGACTGCTATCAAAAGCTGAATACATTTTATCACGTCATAGGAAAGTGGTGTGGGATGTTACTATTTTCAATATCGTCATAAATGTTCCTTGGAGAGATTCAACGTCCGATCCACATAACTTGGTTTTGGAAGCAGGCTCTCTTCTTTTCAGGACTAAATGTGATCTAAGATCTAAGCCATCAGATTTCGAGGAGCAATTTTATACACTGAAAAACTTCTTAACTTCAGTTTCCAGCTGTAATATTTCTCCTTGTGTCCAAATTCAAGATCTCTATGATCACTATGAGGTGAAGTTAAATGAATTTGAGTTGAAGGTAATGATACCTTCTCATTCTTTTCCAATTTCTATCTTGGAGAAAGTTAGTGCTTCTGTCTCCTTTGCATTTTGCTTGATCCAAGATGAGTCAATACTGAAACAGTTGGAGGCTTGTGTCATTGTATCATCACTTCATGCTCATTTTTCTCCATCAATATATGCTGCCATTCTGGGATTGATAGCATATCTAGGTGCTCTGCAATTGAAGTTTGATTCTCTACCCTTGGAAACTATTGATTCGCTTGATGCCACGTCAAATGGATTAGGGACCCCTGTTTTTGGTTTCTCTACCAATATCAAACTGGAGACAGTGAAGATTGAAGTTGAACTGGAAAATGAGCAAGAAAACAGCTCGTCAATCATGCTTAAATTCCAACAATTGGACATCGGCTATTCTCTTTCACAAATTGAGGAATGCTGGATCATAGTGAACGCATTTAGTATCACCACTTATGAACTGGCTAGTAGAAGTGATAGTCGGATTTTGTATTCATCTGGAAATCAGTCATCTACTAATGCTTTACCTCCACATGGGATCGGGGTTGACAATACAAATGATTCTTTTGCCAAAAACGCTGAAAATAATCAAGCATGCTTTACTTTACATTATGAATCTCATCTGAAGGAGCCAGTTCATCACAAATGTAGAATATGCTTGAATAACGGCGATCTCCATTGCTACCCATATGTTATCCGGCTTCTGGTTGCATTTTTTGATAGACTTTCTGCATATGGTTCATCTAATCCTGGTAAGAACACCTCGAGCTCATCTGTGGACGCTAGGTATCCAAACAGTGTTCTAGGTTTTGGGTTTCAAAGGTTTGGTTTCTCCAACTTTGTAGAAATTGGGTCGTCTGAGTATGCAAGCATACCTGTAGATCGTTTTCCATTTGTTATGCTATCCAGTGCTGGTTCCCTTGGTAACCTTGAAAGTTCTTTGGTTTATGCCAGTCCTGAATGGAGAAAATATTTCAACGTAAAGGAGGTATCTAAAACGTTTCATGACCCAGCATTGAAGTTCCGATCTGCTGTTGAAGCTTCTGCTGTGTTTGGGACTTCTGCTACCACTAGTAGTCCTCTTGTAATTGATGTCAATCTCTGTGGATTAAAAGTGCATTTTCATGATTCCAAATGCGTTGTTGGAACGATTACCGTGCCTAGGTGCAACTCTTCAGTATCAATTTATGAAAACTGCTTCGATGTATTGTGTTCGTCTGAGGGGTTAGTTCTCAGCTCATCTTGGTCATCACAGAATTTACGCGAGTTTCTGTGGGGGCCCTCAATATCGAATATCTCTCCTATTCTAAACGTACGTGTGAGGAAAGAATGTGGACCATTGAGTTCACGTGTTGAACTATGCTTCAGCGTGCAGCATGTATATTGCATTTTACCTCCTGAATATCTGGCCATAATAATTGGTTACTTCTCCTTGTCTGATTGGAGTTCCGATTCTAATGACCAGCTTGTCACCACAGGGCATGAAGATACTGAAAGTGATAACGAATGTTCCTTTGTTTACAAGATTGAGATACTGGACTCTGTTTTGATTGTGCCTGTGGAAAGCAATGACGGACAATTTCTAAAGTGTGAACTTGAGCAGTTCTACTGTACATTTATTCAAAGTAGTTTAAATAATGTACTGAAGGACATTCCTCATGAATGTTGGGTCTCCACTGATAAACTTGCAAAGAGGAACCATTCTTTAAATCTATTTGGACGGGACCTGTTTCTTTCATTGCTATCTTTCAAGGACAACCAATACAGTTCCTCTATAAATGTTCCGTTAATTGGTCCTCTTTGTGCTGACATATGGGTGGAAATACCTTGTGAAAACGAATCCTCATGTCAAAGTTCCCCCTCGAACACTTGTGTCATGATCAGAGTTGGAAACTGTCAGCTTAAGCCCGAAGATGATCACTTTTTTCAGGGATTTCAGGGGCTAACTGATATCATAAATCAATTTTCCATAGTTTCTGATCTATCCGAATGTTTCAAGACTGATGTTCTACAGTTTCTTCAATCAAAAAGGTGCCTAGCACAGAATAATGAAGATCCACCTGTACTGTCAAGCGTTAATTATACCGAAGTTAGATGCTATGTCAATTCATTGTCGATACAGTTGAATCCATGCCAAAGAAACTCTGAAGACCCTATCGCTACTGCTGAAATGAAATTAGTTTGCTCGGCATCGTTGAGAAATGACACTCTGTTAAGTGTTGCTATAATTTTTTCTTCCCTAGAATTAAGTTCATTACCTAATTCTGTTGTATTAGCAAGATGCAAGTCCACTAGCACAACCTCGTCGGTTCTTGAATTTTCTCTTACCAAAGAAAAAGACGGTGTAAATGAAGTTCGTGTTTCTTTGCCTTCTGTTGAAGTGTGGTTACATATGTCTTACTGGACTGAGGTGATTGAATTTTTCAAATCTTACGCTGGACAATCATCCACAAGTTTGCCAGACAACTCAGAGCAGGACACTGATGCCCTCATCGTGAGGTCAGATAATATTTTTATTACATTGCACTTTCCTGTTTGGAATTGTGGCCGAGCATTTGGGGAATATCAGGGAGAGGATTGCCATGGATGTGGCTCTACGAATGATTTATCTGACATAGTTGAAGCAAAAGCCTTTAGGTCCATTGCAGTTACTTTGTGTAGTAGAAGTAGTGAACTTTTTGTTGATGGAACGAATGTGAAAGTGAAGTCTGACATAGAAAAAGTAGAGGGCATGGTATGGGTTAGCCAGAATGAAAGTGTTCAATCTACGCCCTTTTTCCAAATATCTCAGGTGCTCTTGGTAGCTGACATTAATAACCAGGAGCTTGTGCATGTTGAAGGAGATGTTCAATGTGATCATTTAGATGTATGGATTTCACACAGCATTCTCTACTTCTGGCATGGTGTACAATTTAGTGTTGCTGAAGGAGGGCATTCCCATTTATCTTTTGGTAGAATCGATGTTGGAGTCCATATTAGGAAGGTTTCTTTTCTGCTAAGCGATGGAAGGTGGAGTTGTAGTGGACCCCTGTTTCAGATTCTTATGGGGAACGTCCCCTTGCATGTTATTGCGACTGAAAATAACATTGAATGTTTAGTTTCTGGTGACCTTCAAGTGAACTACAATAACATTCACAAGGTGTTCTGGGAGCCATTTATTGAGCCTTGGCAATTTGAGGTCAATGTGATTAGAAAACAAGAGATGAGTCTCAGCAGCTCCAATTTGACAGACATCCATCTCAAATCATCTGGACACCTCAATGTGAACTTCACTGAATCCCTCATAGAGTGTCTCTTCAGGACAGTTGAGATGATCAAGGATGCGTGTGTTCTATTGAGTCCAAATGATCTTCCTGAAAGCCAAAAATTATTAAATTCCCCATTTCCTGAGTACACATATGCAGGAAAACATGCTCCTTATGTACTTCAAAACATGACATCGCTGCCTCTAGCATATCATGTTTATCAAGGCCCAATTAGTCCTGATGAGTTTGATTCCTCAGAAATGAATAAAAAATTTGTGCAACCAGGTTCTTTGATTCCAATATATATCAATGATACTCCTGGGAAACAACTTATTCATGTTAAGCCAGCACACTTTCCCGAAAGAATTTTTGATCAGAAGGCAAATGGGGTTCGTCATCAGTATATTAGCATTCAACTTGATGGAACATCAGTGCCTTCAGAACCGATTTCAATGGATCTTGTAGGACTTACCTACTTTGAGGTTGATTTTTCTATGTCATACAATGACAATATGGAAAACCATAGATCAAATGCCACTGCTGGTTTTGTTGTTCCTGTCATATTTGATGTTTCGGTGCAGCGGTACAGCAAATTAATCCGATTGTACTCAACGGTTATACTTTCAAATGCGACTTCAATGCCTTTGGAACTGCGATTTGATATTCCATTTGGTGTGGCCCCAAAGATCTTAGATCCAATATATCCTGGTCAAGAGCTACCGCTGCCTCTGCACTTGGCTGAAGCTGGTAGGATTAGATGGCGTCCAATTGGATATTCTCATCTCTGGAGTGAAGTTTACAATCTCTCAAACCTGCTTTCGCAAGAGGGAAAAATTGGATTTCTCAAGTCTTTTGCTTGCTATCCAGCCCATCCGAATAGTGATCCATTTCGCTGTTGTATCTCAGTAAGAAACGTCAGTATACCTTCTCCTGTTAGGTCAAGAAAGAGTTCATTAAAGCAATCAGTTGCAAATGGTGGTCAAATATTGCACAAGGATGAGGCAAAAAAGCAGTTTATTCACCAAGTAGTCCTCAGTATCCCTTTAGTAGTGAATAACTATCTTCCTGATGCAGTGACATTGACGATAGAAAGTGGTGGGCTTACCCAAACTGCATTTCTTTCAGAGGTGGAAACTTCTTTTCATAATGTTGATCCTTCTCATCAGCTGAAATTGGAGATCCATATTAATGGCTTTAAAACGGCTATTTTGGATTTTCCACGTACTGAAATATTCTGTAAAAAAGCCAAGTTTGGTGGAACGAAGTTTTCACTTTCTGAAGTTGTGCCGTTCGACAGAGACTCGACAAATGGTCCAGTGTATGTCACAGTGGAAAAGGTGATGGATGCATTTTCTGGAGCCAGGGAACTCTTTATCTCTGTTCCCTTTCTGTTATATAATTGCACGGGTTTTCCACTGTTTATTTCTGAATCTGCCAGTGACATGAAAGGAGTTAGCTGCATTGTGCCTTCTTGTTATGATATGGATGAACAAGAAGTATTTCAGGGAAACAAGGATGGTCTTGGTCTGGTATCTTCTAGCTATAATCCTAATGCTAGAGAGTCGCATACAATTGGAAGTTCTTCGTCAAGTGTTCATATAGTCTCAAATAGAGACAATGCTGCAACTCACAAAGGAATAAGTTCTAGCAAACCTCTGTACTCCGAAGACAACTTTAATGAACTTTTAAGCAAATGTAATGTAGACAGGGAAAACTCAGTTTCCAACGGTTTACAGAATAGATCAAGTTCGACCAGTCAGTTGGCCTCAAAAGATTTAAACTCTAGTGGATATGAGCGTGGAAGAGTGAGGGCATGCATGTTCTCTCCTAATCAGTTTTCCTCTGCTGGTGAAGTAATGGTTAGAGTGAGTAGGTGTATGCCTGAATATGTAAGAGATAAGATGCCAAATTCTCTATGGTCAAGTTCATTTTCCCTTATTCCACCTAGTGGTTCAACTACTGTCCTTGTTCCCCAACCGTCAACAAATCAGGCTTTTATGATGTCCATTACGTCCAGTGCAGTGGCCGCACCATTTGCTGGGAGGACAAGTGCAATTACCTTCCAACCTAGGTACATAATCAGCAACGCATGCAGTAAGAACATATGTTATAAGCAGAAAGGAACCGAATTTTCTTTTCAATTGGGTACAGGGGAACATTCTCATCTTCACTGGATGGATACAACCAGGGAGTTACTCGTCTCCATTCGTTATAATGAGCCTGGATGGCAATGGTCAGGTGGCTTCTTGCCAGACCATCTCGGTGACACTCAAGTGAAAATGCGAAATTATCTCTCTGGTTCCTTAAATATGATACGGGTTGAAGTGCAAAATGCTGATGTATCTTTGGGAGATGAAACGATTGTTGGAAACTTCCATGGAAATTCTGGGACCAACTTGATTCTTATTTCAGATGATGAGACTGGGTATATGCCCTACAGGGTTGATAATTTTTCGAATGAGAGGCTAAGGATCTACCAACAAAAGTGTGAAACCTTTGAAACTATTGTTCAGTCTTACACTTCTTGCCCTTATGCCTGGGATGAACCATGCTATCCGCATCGTCTAACTGTGGAGGTACCGGGTAAGCGTGTTTTAGGGTCATATGCTCTTGATGATGTAAAACAATACAGTCCGGTGCAATTACCATCATCTCCCGAGAAACCTGAGAGAACATTGCATATATCTATCCATGTTGAGGGAGCAACAAAGGTCCTGTGTGTTATAGATTCAAGTTACCATGTTTTGAATGACAACAAGAGTCTACCTCACTCAAAAAACAAAGGGAAACATGAACAGAAACAGGACAAGTTTTTTGGTTATATGGAAAGATTTTCATTTTTTATTCAAGAGATTGGTATCTCTTTGATCAATATACACCCTCAGGAGTTGCTTTTCATATGTGCAAAGAACATTACAGCTGATCTGGTACAGAGTTTGGATCAACAAAAACTTTCATTCCAAATCGAATCATTGCAAATCGATAATCAGCTTCGATCTTCCCCATATCCTGTTATGTTGTCTTTTGATCGTGAGTACAAAAGCAATCCAGCTGGCCATGTGATTAGGGAGGATGACATGAAACCAAGTGAAAGGATATTGCAAAGACCTTCTCACAATTTTGAACCTATATTCTGCCTAACTGTATCCAAGTGGAGGAAGAAAGATGTCTCATTGGTTTCATTTGAATACATAAGCTTAAGGGTTGCAGATGTTTGTCTTGAGCTTGAACAAGAATTGATATTAAGCCTATTTGGATTCATTAGAAATGTATCTTCAAGGTTCCAGAGTGGAGTCTTGCCATTATCAGATCCGTTCCTGCACCCTCCTAATGATGCTGGTTCAATGGATTCATATGCAACTGATAACCAACTACACTTAATGAATGTTCCTCTGTTTACTGAAATCCATAGGCAGAGACTCTCACTGCCTTCAATAGTTCCCATTGGAGCTCCTTGGCAGCAAATTTACCTTTTGGCTAGAAGACAGAAAAAGATATATGTAGAAATGTTTGAATTGTCCCCCATCAAGTTGACCTTAAGCTTTTCCAGCACTCCATGGATGCTTAGAAATGGGATTCTTGCAGCAGGAGAATCTGTTATCCATAGAGGTCTGATGGCTCTTGCTGATGTGGAGGGTGCTCGAATTCATCTGAAACAGTTAACAATTGCACATCAAATTGCTAGCTTGGAGTCTCTACAGGAGATCCTTTTGAGGCATTATACTCGGCAACTTCTTCATGAGATGTATAAGGTGTTTGGTTCAGCCGGTGTAATTGGCAATCCCATGGGATTTGCAAGGAGTTTAGGGCTTGGCATTAGAGACTTCTTGTCAGTGCCTGCCAGAAGCATTTTTCAGAGCCCTACTGGGTTAATTACTGGCATGGCACAAGGCACGACTAGTCTCCTAAGCAATACTGTCTATGCTATAAGTGATGCTGCCACCCAATTCAGTAAAGCTGCACACAAGGGAATTGTGGCATTTACGTTTGATGACCAAGCAGTTTCAGAAGTACAACAGCAACAGACTGGTATAACATCTCACTCTAAAGGTGTCATCAATGAAGTACTTGAGGGACTCACAGGTCTTCTTCAATCACCGATCAATGGAGCTGAGAAACATGGTCTTCCTGGTGTGCTCTCAGGCATAGCTTTAGGCCTTACAGGACTTGTGGCCAAACCGGCTGCTAGTATTCTTGAAGTTACTGGAAAAACTGCGCAGAGCATTAGAAACCGAAGTAGGATCTATCAAACGAGACAACAGCGCTTTAGAGTCCGTCTTCCAAGGCCTCTAAGCCAAGAATATCCCTTAAGACCTTACTGTTGGGAAGAAGCTGTTGGAGCATCAGTACTTGTTGAGGCAGATGGTAATTTGAGGCTCAAGGATGAAATATTTGTGACATGCAAGAAGCTTAAAGAAGCTGGTAAATTTGTCATCATCACAGGGAGACTGGTTTTGATTGTAAGCTGCTCAAGTTTGGTGGACTTGGGTAAGCCTGAATTTCGTGGTGTTCCTTCTGATCTCGAATGGGTGATAGAATCAGAAATTCATTTGGAGAGTGTGATACATGCTGACTGTGATCAAGGAGTAGTGCATATAGTCGGCAGCAGCTCAAATACGCCTTTGCGCCAAAACCAGCTAGCCAAAAGAAGCAGTGGAACAAGGGCGGTGCGTTGGAACAATCCTACTGTTCCCCTCATTCAAACTAACTTAGAACTAGAACACAAAGATGCGGAAAACTTGTTACAGGTTTTGTCTTCTACAATTGAACTGGGAAAAGACCAAGGTTGGGGTTGTAGGAATATTCTGCACCGAAGTAATATCAAATAG

Protein Analysis

3422

Amino Acids

383.28

Weight (kDa)

5.73

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_N PF12624 7 - 703 9.2e-68 VPS13-like family N-terminal region
VPS13_M PF25033 1730 - 1808 7.5e-08 VPS13-like family middle region
VPS13_VAB PF25036 1976 - 2263 6.7e-08 Vacuolar-sorting associated protein 13, adaptor binding domain
VPS13_VAB PF25036 2363 - 2636 1.3e-26 Vacuolar-sorting associated protein 13, adaptor binding domain
VPS13_C PF25037 3210 - 3279 2.6e-08 Intermembrane lipid transfer protein VPS13, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1089, 7548, 7650
AarI CACCTGC 1 cut(s) 1684
AasI GACNNNNNNGTC 2 cut(s) 3659, 9519
Acc16I TGCGCA 2 cut(s) 987, 9654
Acc36I ACCTGC 2 cut(s) 1684, 6258
Acc65I GGTACC 1 cut(s) 8014
AccB1I GGYRCC 2 cut(s) 4224, 8014
AccB7I CCANNNNNTGG 4 cut(s) 681, 2575, 7385, 7475
AccI GTMKAC 4 cut(s) 1879, 6358, 7140, 8200
AccII CGCG 1 cut(s) 3456
AclI AACGTT 1 cut(s) 3191
AcoI YGGCCR 7 cut(s) 357, 1498, 3600, 4738, 7461, 8491, 9609
AcvI CACGTG 1 cut(s) 3542
AcyI GRCGYC 1 cut(s) 6199
AdeI CACNNNGTG 3 cut(s) 737, 5332, 7385
AfeI AGCGCT 1 cut(s) 9701
AflII CTTAAG 4 cut(s) 4094, 8635, 8974, 9745
AflIII ACRYGT 4 cut(s) 373, 890, 3508, 3541
AhdI GACNNNNNGTC 2 cut(s) 6747, 9265
AhlI ACTAGT 2 cut(s) 3263, 9335
AjiI CACGTC 2 cut(s) 1913, 2448
AjuI GAANNNNNNNTTGG 8 cut(s) 6196, 6228, 6537, 6569, 7785, 7817, 9756, 9788
AleI CACNNNNGTG 1 cut(s) 7709
Alw21I GWGCWC 7 cut(s) 1447, 2392, 2963, 4989, 8890, 9079, 9576
Alw44I GTGCAC 1 cut(s) 1443
AlwNI CAGNNNCTG 1 cut(s) 9275
Ama87I CYCGRG 3 cut(s) 1715, 2956, 8093
Aor51HI AGCGCT 1 cut(s) 9701
ApaI GGGCCC 2 cut(s) 577, 3474
ApaLI GTGCAC 1 cut(s) 1443
ArsI GACNNNNNNTTYG 3 cut(s) 32, 8234, 8266
AseI ATTAAT 4 cut(s) 1461, 5004, 6032, 6624
Asp700I GAANNNNTTC 6 cut(s) 2093, 4950, 6385, 6936, 7018, 7078
Asp718I GGTACC 1 cut(s) 8014
AspA2I CCTAGG 3 cut(s) 40, 3353, 7505
AspLEI GCGC 4 cut(s) 988, 9655, 9702, 10065
AsuC2I CCSGG 1 cut(s) 8019
AsuII TTCGAA 2 cut(s) 6, 7892
AsuNHI GCTAGC 2 cut(s) 9119, 10075
AvaI CYCGRG 3 cut(s) 1715, 2956, 8093
AvrII CCTAGG 3 cut(s) 40, 3353, 7505
AxyI CCTNAGG 1 cut(s) 8319
BaeGI GKGCMC 3 cut(s) 577, 1447, 3474
BalI TGGCCA 5 cut(s) 359, 1500, 3602, 8493, 9611
BanI GGYRCC 2 cut(s) 4224, 8014
BanII GRGCYC 6 cut(s) 231, 577, 2963, 3474, 8890, 9299
BarI GAAGNNNNNNTAC 4 cut(s) 2310, 2342, 4563, 4595
BbrPI CACGTG 1 cut(s) 3542
BbsI GAAGAC 8 cut(s) 247, 4347, 7110, 8926, 9515, 9554, 9707, 10185
Bbv12I GWGCWC 7 cut(s) 1447, 2392, 2963, 4989, 8890, 9079, 9576
BceAI ACGGC 5 cut(s) 26, 49, 2875, 6654, 6709
BcgI CGANNNNNNTGC 6 cut(s) 3331, 3365, 6058, 6092, 9143, 9177
BciVI GTATCC 4 cut(s) 2991, 6485, 7609, 8593
BclI TGATCA 9 cut(s) 1117, 2155, 4071, 4108, 5047, 5505, 5809, 8304, 10015
BcnI CCSGG 1 cut(s) 8019
BcuI ACTAGT 2 cut(s) 3263, 9335
BfmI CTRYAG 8 cut(s) 348, 1206, 1536, 1563, 3069, 4202, 7873, 9134
BfoI RGCGCY 1 cut(s) 9703
BfrI CTTAAG 4 cut(s) 4094, 8635, 8974, 9745
BfuAI ACCTGC 2 cut(s) 1684, 6258
BfuI GTATCC 4 cut(s) 2991, 6485, 7609, 8593
BglI GCCNNNNNGGC 2 cut(s) 7663, 9617
BglII AGATCT 3 cut(s) 2048, 2146, 6123
BlnI CCTAGG 3 cut(s) 40, 3353, 7505
BlpI GCTNAGC 1 cut(s) 5189
BmcAI AGTACT 4 cut(s) 800, 1138, 9504, 9786
BmeRI GACNNNNNGTC 2 cut(s) 6747, 9265
BmeT110I CYCGRG 3 cut(s) 1715, 2956, 8093
BmgBI CACGTC 2 cut(s) 1913, 2448
BmrI ACTGGG 3 cut(s) 7869, 9312, 10217
BmtI GCTAGC 2 cut(s) 9123, 10079
BmuI ACTGGG 3 cut(s) 7869, 9312, 10217
BpiI GAAGAC 8 cut(s) 247, 4347, 7110, 8926, 9515, 9554, 9707, 10185
BplI GAGNNNNNCTC 2 cut(s) 8188, 8220
BpmI CTGGAG 7 cut(s) 134, 949, 1320, 1433, 2519, 6244, 6813
Bpu10I CCTNAGC 2 cut(s) 7904, 9344
Bpu1102I GCTNAGC 1 cut(s) 5189
Bpu14I TTCGAA 2 cut(s) 6, 7892
BpuEI CTTGAG 9 cut(s) 62, 544, 3809, 6266, 7692, 8678, 9527, 9800, 9892
BpuMI CCSGG 1 cut(s) 8019
Bsa29I ATCGAT 2 cut(s) 5151, 8418
BsaAI YACGTR 5 cut(s) 909, 1484, 3509, 3542, 6659
BsaBI GATNNNNATC 3 cut(s) 86, 3288, 8294
BsaHI GRCGYC 1 cut(s) 6199
BsaI GGTCTC 1 cut(s) 3868
BsaWI WCCGGW 1 cut(s) 8071
BsaXI ACNNNNNCTCC 8 cut(s) 5197, 5227, 7471, 7501, 9018, 9048, 9768, 9798
Bse118I RCCGGY 3 cut(s) 637, 9203, 9616
Bse21I CCTNAGG 1 cut(s) 8319
Bse3DI GCAATG 8 cut(s) 2869, 3766, 3929, 4712, 4833, 7673, 8111, 8408
Bse8I GATNNNNATC 3 cut(s) 86, 3288, 8294
BseCI ATCGAT 2 cut(s) 5151, 8418
BseJI GATNNNNATC 3 cut(s) 86, 3288, 8294
BseMI GCAATG 8 cut(s) 2869, 3766, 3929, 4712, 4833, 7673, 8111, 8408
BseRI GAGGAG 1 cut(s) 2084
BseSI GKGCMC 3 cut(s) 577, 1447, 3474
BseYI CCCAGC 3 cut(s) 392, 3202, 7475
BsgI GTGCAG 7 cut(s) 3584, 6035, 6152, 7476, 8741, 9381, 10229
Bsh1236I CGCG 1 cut(s) 3456
BshNI GGYRCC 2 cut(s) 4224, 8014
BshVI ATCGAT 2 cut(s) 5151, 8418
BsiHKAI GWGCWC 7 cut(s) 1447, 2392, 2963, 4989, 8890, 9079, 9576
BsiHKCI CYCGRG 3 cut(s) 1715, 2956, 8093
BsiSI CCGG 6 cut(s) 638, 2890, 8018, 8072, 9204, 9617
BsiWI CGTACG 1 cut(s) 3505
BslFI GGGAC 9 cut(s) 683, 909, 2476, 3262, 3929, 5231, 7840, 8941, 9525
BsmBI CGTCTC 2 cut(s) 1646, 7642
BsmFI GGGAC 9 cut(s) 683, 909, 2476, 3262, 3929, 5231, 7840, 8941, 9525
BsmI GAATGC 4 cut(s) 2615, 5076, 5125, 5847
Bso31I GGTCTC 1 cut(s) 3868
BsoBI CYCGRG 3 cut(s) 1715, 2956, 8093
Bsp119I TTCGAA 2 cut(s) 6, 7892
Bsp120I GGGCCC 2 cut(s) 573, 3470
Bsp1407I TGTACA 3 cut(s) 887, 3803, 5098
Bsp1720I GCTNAGC 1 cut(s) 5189
Bsp19I CCATGG 5 cut(s) 206, 4774, 7813, 8993, 9222
BspDI ATCGAT 2 cut(s) 5151, 8418
BspFNI CGCG 1 cut(s) 3456
BspHI TCATGA 5 cut(s) 3196, 3316, 3850, 4068, 9175
BspMAI CTGCAG 3 cut(s) 352, 1210, 1567
BspMI ACCTGC 2 cut(s) 1684, 6258
BspOI GCTAGC 2 cut(s) 9123, 10079
BspT104I TTCGAA 2 cut(s) 6, 7892
BspT107I GGYRCC 2 cut(s) 4224, 8014
BspTI CTTAAG 4 cut(s) 4094, 8635, 8974, 9745
BspTNI GGTCTC 1 cut(s) 3868
BsrDI GCAATG 8 cut(s) 2869, 3766, 3929, 4712, 4833, 7673, 8111, 8408
BsrFI RCCGGY 3 cut(s) 637, 9203, 9616
BsrGI TGTACA 3 cut(s) 887, 3803, 5098
BssAI RCCGGY 3 cut(s) 637, 9203, 9616
BssNAI GTATAC 1 cut(s) 6359
BssNI GRCGYC 1 cut(s) 6199
Bst1107I GTATAC 1 cut(s) 6359
Bst6I CTCTTC 7 cut(s) 279, 562, 1010, 2025, 3370, 5492, 7234
BstACI GRCGYC 1 cut(s) 6199
BstAFI CTTAAG 4 cut(s) 4094, 8635, 8974, 9745
BstAPI GCANNNNNTGC 2 cut(s) 7472, 7527
BstAUI TGTACA 3 cut(s) 887, 3803, 5098
BstBAI YACGTR 5 cut(s) 909, 1484, 3509, 3542, 6659
BstBI TTCGAA 2 cut(s) 6, 7892
BstDSI CCRYGG 6 cut(s) 206, 1258, 4774, 7813, 8993, 9222
BstEII GGTNACC 2 cut(s) 3116, 5297
BstFNI CGCG 1 cut(s) 3456
BstH2I RGCGCY 1 cut(s) 9703
BstHHI GCGC 4 cut(s) 988, 9655, 9702, 10065
BstPI GGTNACC 2 cut(s) 3116, 5297
BstSFI CTRYAG 8 cut(s) 348, 1206, 1536, 1563, 3069, 4202, 7873, 9134
BstSLI GKGCMC 3 cut(s) 577, 1447, 3474
BstSNI TACGTA 2 cut(s) 909, 1484
BstUI CGCG 1 cut(s) 3456
BstV2I GAAGAC 8 cut(s) 247, 4347, 7110, 8926, 9515, 9554, 9707, 10185
BstXI CCANNNNNNTGG 4 cut(s) 4781, 6626, 7198, 7459
BstZ17I GTATAC 1 cut(s) 6359
Bsu15I ATCGAT 2 cut(s) 5151, 8418
Bsu36I CCTNAGG 1 cut(s) 8319
BsuI GTATCC 4 cut(s) 2991, 6485, 7609, 8593
BsuTUI ATCGAT 2 cut(s) 5151, 8418
BtgI CCRYGG 6 cut(s) 206, 1258, 4774, 7813, 8993, 9222
BtgZI GCGATG 2 cut(s) 5208, 5616
BtrI CACGTC 2 cut(s) 1913, 2448
BtsI GCAGTG 5 cut(s) 5973, 6516, 7464, 8861, 10096
BveI ACCTGC 2 cut(s) 1684, 6258
CaiI CAGNNNCTG 1 cut(s) 9275
CciI TCATGA 5 cut(s) 3196, 3316, 3850, 4068, 9175
CfoI GCGC 4 cut(s) 988, 9655, 9702, 10065
Cfr10I RCCGGY 3 cut(s) 637, 9203, 9616
ClaI ATCGAT 2 cut(s) 5151, 8418
CseI GACGC 2 cut(s) 2981, 6188
CsiI ACCWGGT 1 cut(s) 5719
DraI TTTAAA 4 cut(s) 3825, 3900, 6634, 7215
DraIII CACNNNGTG 3 cut(s) 737, 5332, 7385
DrdI GACNNNNNNGTC 2 cut(s) 3659, 9519
DriI GACNNNNNGTC 2 cut(s) 6747, 9265
DseDI GACNNNNNNGTC 2 cut(s) 3659, 9519
EaeI YGGCCR 7 cut(s) 357, 1498, 3600, 4738, 7461, 8491, 9609
Eam1104I CTCTTC 7 cut(s) 279, 562, 1010, 2025, 3370, 5492, 7234
Eam1105I GACNNNNNGTC 2 cut(s) 6747, 9265
EarI CTCTTC 7 cut(s) 279, 562, 1010, 2025, 3370, 5492, 7234
Ecl136II GAGCTC 2 cut(s) 2961, 8888
Eco105I TACGTA 2 cut(s) 909, 1484
Eco147I AGGCCT 2 cut(s) 9594, 9724
Eco24I GRGCYC 6 cut(s) 231, 577, 2963, 3474, 8890, 9299
Eco31I GGTCTC 1 cut(s) 3868
Eco32I GATATC 1 cut(s) 4144
Eco47III AGCGCT 1 cut(s) 9701
Eco53kI GAGCTC 2 cut(s) 2961, 8888
Eco72I CACGTG 1 cut(s) 3542
Eco81I CCTNAGG 1 cut(s) 8319
Eco88I CYCGRG 3 cut(s) 1715, 2956, 8093
Eco91I GGTNACC 2 cut(s) 3116, 5297
EcoICRI GAGCTC 2 cut(s) 2961, 8888
EcoO109I RGGNCCY 9 cut(s) 25, 573, 1640, 1840, 2463, 3470, 3471, 3916, 8149
EcoO65I GGTNACC 2 cut(s) 3116, 5297
EcoRI GAATTC 1 cut(s) 9081
EcoRV GATATC 1 cut(s) 4144
EcoT22I ATGCAT 2 cut(s) 6787, 7256
EcoT38I GRGCYC 6 cut(s) 231, 577, 2963, 3474, 8890, 9299
Esp3I CGTCTC 2 cut(s) 1646, 7642
FalI AAGNNNNNCTT 2 cut(s) 8957, 8989
FaqI GGGAC 9 cut(s) 683, 909, 2476, 3262, 3929, 5231, 7840, 8941, 9525
FauI CCCGC 1 cut(s) 659
FauNDI CATATG 9 cut(s) 2881, 2926, 4004, 4584, 5590, 7541, 8041, 8336, 8791
FbaI TGATCA 9 cut(s) 1117, 2155, 4071, 4108, 5047, 5505, 5809, 8304, 10015
FblI GTMKAC 4 cut(s) 1879, 6358, 7140, 8200
FriOI GRGCYC 6 cut(s) 231, 577, 2963, 3474, 8890, 9299
FspI TGCGCA 2 cut(s) 987, 9654
GlaI GCGC 4 cut(s) 987, 9654, 9701, 10064
GsaI CCCAGC 3 cut(s) 396, 3206, 7479
GsuI CTGGAG 7 cut(s) 134, 949, 1320, 1433, 2519, 6244, 6813
HaeII RGCGCY 1 cut(s) 9703
HapII CCGG 6 cut(s) 638, 2890, 8018, 8072, 9204, 9617
HgaI GACGC 2 cut(s) 2981, 6188
HhaI GCGC 4 cut(s) 988, 9655, 9702, 10065
Hin1I GRCGYC 1 cut(s) 6199
Hin6I GCGC 4 cut(s) 986, 9653, 9700, 10063
HinP1I GCGC 4 cut(s) 986, 9653, 9700, 10063
HincII GTYRAC 8 cut(s) 583, 763, 1081, 1880, 2740, 7419, 8970, 9099
HindII GTYRAC 8 cut(s) 583, 763, 1081, 1880, 2740, 7419, 8970, 9099
HindIII AAGCTT 5 cut(s) 215, 3231, 8632, 8977, 9845
HpaI GTTAAC 3 cut(s) 583, 1081, 9099
HpaII CCGG 6 cut(s) 638, 2890, 8018, 8072, 9204, 9617
Hpy99I CGWCG 1 cut(s) 4501
Hsp92I GRCGYC 1 cut(s) 6199
HspAI GCGC 4 cut(s) 986, 9653, 9700, 10063
KflI GGGWCCC 1 cut(s) 2463
KpnI GGTACC 1 cut(s) 8018
Ksp22I TGATCA 9 cut(s) 1117, 2155, 4071, 4108, 5047, 5505, 5809, 8304, 10015
KspAI GTTAAC 3 cut(s) 583, 1081, 9099
MabI ACCWGGT 1 cut(s) 5719
MfeI CAATTG 8 cut(s) 1125, 2396, 2573, 6204, 7008, 7577, 9102, 10200
MlsI TGGCCA 5 cut(s) 359, 1500, 3602, 8493, 9611
MluNI TGGCCA 5 cut(s) 359, 1500, 3602, 8493, 9611
Mox20I TGGCCA 5 cut(s) 359, 1500, 3602, 8493, 9611
Mph1103I ATGCAT 2 cut(s) 6787, 7256
MroXI GAANNNNTTC 6 cut(s) 2093, 4950, 6385, 6936, 7018, 7078
MscI TGGCCA 5 cut(s) 359, 1500, 3602, 8493, 9611
Msp20I TGGCCA 5 cut(s) 359, 1500, 3602, 8493, 9611
MspA1I CMGCKG 9 cut(s) 347, 812, 1205, 2115, 6019, 6160, 6604, 8357, 8489
MspCI CTTAAG 4 cut(s) 4094, 8635, 8974, 9745
MspI CCGG 6 cut(s) 638, 2890, 8018, 8072, 9204, 9617
MunI CAATTG 8 cut(s) 1125, 2396, 2573, 6204, 7008, 7577, 9102, 10200
Mva1269I GAATGC 4 cut(s) 2615, 5076, 5125, 5847
MvnI CGCG 1 cut(s) 3456
NciI CCSGG 1 cut(s) 8019
NcoI CCATGG 5 cut(s) 206, 4774, 7813, 8993, 9222
NdeI CATATG 9 cut(s) 2881, 2926, 4004, 4584, 5590, 7541, 8041, 8336, 8791
NheI GCTAGC 2 cut(s) 9119, 10075
NmeAIII GCCGAG 3 cut(s) 4358, 4766, 9142
NsbI TGCGCA 2 cut(s) 987, 9654
NsiI ATGCAT 2 cut(s) 6787, 7256
NspV TTCGAA 2 cut(s) 6, 7892
OliI CACNNNNGTG 1 cut(s) 7709
PaeI GCATGC 3 cut(s) 2790, 7254, 7530
PaeR7I CTCGAG 2 cut(s) 1715, 2956
PagI TCATGA 5 cut(s) 3196, 3316, 3850, 4068, 9175
PaqCI CACCTGC 1 cut(s) 1684
PceI AGGCCT 2 cut(s) 9594, 9724
PciI ACATGT 1 cut(s) 890
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PctI GAATGC 4 cut(s) 2615, 5076, 5125, 5847
PdmI GAANNNNTTC 6 cut(s) 2093, 4950, 6385, 6936, 7018, 7078
Pfl23II CGTACG 1 cut(s) 3505
PflMI CCANNNNNTGG 4 cut(s) 681, 2575, 7385, 7475
PmaCI CACGTG 1 cut(s) 3542
PmlI CACGTG 1 cut(s) 3542
Ppu21I YACGTR 5 cut(s) 909, 1484, 3509, 3542, 6659
PpuMI RGGWCCY 5 cut(s) 25, 1840, 2463, 3916, 8149
PscI ACATGT 1 cut(s) 890
PshBI ATTAAT 4 cut(s) 1461, 5004, 6032, 6624
PsiI TTATAA 3 cut(s) 1089, 7548, 7650
Psp124BI GAGCTC 2 cut(s) 2963, 8890
Psp1406I AACGTT 1 cut(s) 3191
Psp5II RGGWCCY 5 cut(s) 25, 1840, 2463, 3916, 8149
PspCI CACGTG 1 cut(s) 3542
PspEI GGTNACC 2 cut(s) 3116, 5297
PspFI CCCAGC 3 cut(s) 392, 3202, 7475
PspLI CGTACG 1 cut(s) 3505
PspOMI GGGCCC 2 cut(s) 573, 3470
PspPPI RGGWCCY 5 cut(s) 25, 1840, 2463, 3916, 8149
PspXI VCTCGAGB 1 cut(s) 2956
PsrI GAACNNNNNNTAC 4 cut(s) 1297, 1329, 4530, 4562
PstI CTGCAG 3 cut(s) 352, 1210, 1567
PstNI CAGNNNCTG 1 cut(s) 9275
PvuII CAGCTG 4 cut(s) 2115, 6604, 8357, 8489
SacI GAGCTC 2 cut(s) 2963, 8890
SalI GTCGAC 1 cut(s) 1878
ScaI AGTACT 4 cut(s) 800, 1138, 9504, 9786
SexAI ACCWGGT 1 cut(s) 5719
SfcI CTRYAG 8 cut(s) 348, 1206, 1536, 1563, 3069, 4202, 7873, 9134
Sfr274I CTCGAG 2 cut(s) 1715, 2956
SfuI TTCGAA 2 cut(s) 6, 7892
SlaI CTCGAG 2 cut(s) 1715, 2956
SnaBI TACGTA 2 cut(s) 909, 1484
SpeI ACTAGT 2 cut(s) 3263, 9335
SphI GCATGC 3 cut(s) 2790, 7254, 7530
SseBI AGGCCT 2 cut(s) 9594, 9724
SspI AATATT 8 cut(s) 499, 2122, 3164, 4702, 6423, 6669, 9828, 10240
SstI GAGCTC 2 cut(s) 2963, 8890
StuI AGGCCT 2 cut(s) 9594, 9724
TauI GCSGC 2 cut(s) 347, 7466
TspGWI ACGGA 7 cut(s) 793, 927, 1331, 3780, 3966, 8739, 9701
Van91I CCANNNNNTGG 4 cut(s) 681, 2575, 7385, 7475
Vha464I CTTAAG 4 cut(s) 4094, 8635, 8974, 9745
VneI GTGCAC 1 cut(s) 1443
VspI ATTAAT 4 cut(s) 1461, 5004, 6032, 6624
XcmI CCANNNNNNNNNTGG 6 cut(s) 754, 2494, 2650, 5138, 7350, 7820
XhoI CTCGAG 2 cut(s) 1715, 2956
XmaJI CCTAGG 3 cut(s) 40, 3353, 7505
XmiI GTMKAC 4 cut(s) 1879, 6358, 7140, 8200
XmnI GAANNNNTTC 6 cut(s) 2093, 4950, 6385, 6936, 7018, 7078
ZrmI AGTACT 4 cut(s) 800, 1138, 9504, 9786
Zsp2I ATGCAT 2 cut(s) 6787, 7256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.