RchiOBHm_Chr2g0148311

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001657
Physical Location & Seq
Forward (+)
65929980 .. 65952796
22817 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51789

Sequence Viewer

Length: 9351 bp
ATGTTATGGTGGCATTATGCGCAAGAATCTATCTTATCAGATGTACGGAAGAGATTAAAGAAAACATCGTGGAGACACTTCGGACAGAGACTAAGCTCTTGTCGGAAGTATGTTAACCTATATAAGACTAAGCTGGATTTTCTTCGCCATGATCAGCCAATTGATGAAAGTACTCGATGGGAATTGGAGCAGATGGAGAAAGAATTAGACATAGATGATATTTTAAGTTACAGATCGGCTGCAGAATGTGAGTTGCAGGAGTTTTTGGTGAACTCTTCAACTGGAAAATCCCGTGGATGGCTGAACTGGCTATCTCTTGGTATGCTTGGTGCTGGAGGAACAGATGACTTGGGTCAATTTTCTGGGGCTGTTTTTGTTTCTGATGCAGTTATTAAGGATATATATGAGGCGACAAAGTTCAATCCTCCTATTTTATCCAATGGGGATGCTCGAACAACTGATAAGATTGATATATGCGCACTCCATTTCAGCATCAATCGGATTTCTGCAACACTGCGTAACATGAAGTATGGCCAAGAAATTGCAGAGTTGATGTTGAATGGGGTGACTACGGAGTGCAAATTTAGGGAAGAATCTGCAGCCATTGTCACCATTGTGAACTCAGGGCAGATGGTTTATCCTTGTAACAAGAAAGTCATTCTGCATTTGCGAAGGCCTAATAATGAGAAGAACGAAGTGGAAAACGAGAATCCTTCCTGTAGATTGCAAGTGGATGTATCATCAAACCTCGAGGCTGAATTGTCCATTAAGGGGATGCTTCAACCATTAGAAGTCACCATTGATGCAGAATTTTTCTTAAAGTTGATGGATTTTCTTGGAGCTTTAAAATCCTTTGAAAGTCAGCATGGAAGGGTCCTTCTGTCGCTAAATGGGATAGAAAATGTCAATGGTCGACTGTTGTCGAAAGCTGAATACCTTTTATCACATCATAGGAAAGTGGTGTGGGATGTTACTATCTTCAATATCATCATAAATGTTCCTTGGAGAGAGGCAATATCTGATCCACATAACTTGGTTTTTGAAGCAGGCTCTCTTCTTTTTAGGACTAAATGTGATATGGGATCTCAGCCATCAGATGATGAGGAACAATTTTATACTCTGAAAAAGTTCTTAACTTCAGTTTCCAGCTGTAATATTTCTCCATGCGTCCAAATTCAAGATCTGTACGATCACTATGAGGTCAAGTTAAATGATTTTGAGTTGAAGGTAACGATGCCTTCTCATTCTTCTCCAATTTCTATCTTGGAGAAACTTAGTCCTTCTGTCTCCTTTGCATTTTGCTTGATCCAAGATGAGTCAATATTGAAACAGTTGGAGGCTTGTGTCATTGTATCATCACTTCATGCACATTTTTCTCCATCAATATATGCTGCAATGCTGGGATTGATAGAATATCTAGGTGCTCTGCAAGTGAATTTTGATTCTCTACCCTTGGGAACTATTGATTCACTTGATGCCACATCAAATGGATTAGGGAATCCTGTTTTCGGTTTCTCTACCAATATCAAACTGGAGACAGTGAAGATTGAAGTCGAACTGGAAAATGAAGAAGAAAATAGCTCGTCATTCATGGTTAAATTCCAACAATTGGACATGGGTTATTCGCTTTCAAAAATTGAGGAATGCTGGATCTTAATGAACGCATTGAGTATCACCACTTCTAAATTGGCTGATAAAAGTGATAGTCGGATTTTGTATTCATCTGGAAATCAATCATCTATGAATGCTTTACATCCACATGAGATTGGGATTGGCAATAGAAATGATCATTTTGCCAACACCATTGGAAATAATGAAGCATGCTTTACTTTACAATATGAATCTCATCCGAAGGAGACTGTTCGTAAAAAATGTAGAATATGCTTGAATAACGGTGATCTCCACTGCTACCCATATGTTATCCGGCTTCTGGTTGCATTTTTTGATAAACTTTCTACTTATGGTGCATCTAATCCTGGTAAGAACACTTTGAGCTCATCTGTGGATGCTAGGTATCCAAAAAGTGTTCCTGGTTTTGGGTCTCAAAGGTTTGGTTTCTCCAACTTTGTAGAAACTGGGTCCTCTGAGTATGCAAGCATACCTTTAGATCATTTTCCTTTTGTTATGCTATCCAATGCTGGTTCCCTTGGTAACCTTGAAAGTTCTTTGCTTTATGCCAGTCCCGAGTGGAGAAAGTATTTCAGTATAAGGGACAGCAGAATCAGCAGCCCACTATCCAGCATAAAGGAGGTATCTAAAAAGTTCCATGCCCCAGCATTGAAGCACTCATCTGTGGTTGAAGCTTCTGCTGCGTGTGGGAGTTCTGCCACTACTAGTAGTCCGCTTGTAATTGATGTCAATCTCTGTGGAATCAGAGTGCATTTTCATGATTCAAAATGCATTGTTGGGACAATTACCGTGCCTAGGTCCAACTCTTCAGTATTAATTTCTGAAAACAGCTTTGATATATTGTGTTCTACTGAGGGGTTAGTTCTCAGTTCATCTTGGTCGTCACAGAATTTACGCGAGTTTCTGTGGGGCCCCTCGGTCTTGAATTTATCTCCTATTTTAAATGTACGTGTGAGGAAAGAATGTGGGCCATTGAGTTCGCATGTTGAACTATGCTTTGGCATACAGCATGTATATTGCATCTTACCTCCTGAATATCTGGCCATAATAATTGGTTACTTTTCATCGTCTGATTGGAGTTCAGATTCTAATGACCAGCTTGTCACCATGGGGCATGAAGATACTGAAAGTGAAAGTGAATGTTCCTTTATCTACAAGATTGAGATACTGGACTCTGTTTTAGTTGTGCCCGTGGAAAGCAACGAGGGTCAGTTTCTAAAGAGCGAACTTCAGCAGTTCTACTGTACATTTATTCAAAGCAGTTCAAATAATGTATTGAAGGACATTCCCCATGAATGTTGGGTCCCGACTGATAAACTTGCTAAAAGAAATCATTGTTTAAATTTATTTGGACGAGACTTGTTTCTATCATTCCTGTCTTTCAAGGACAATCAATACAATTCCTTTGTAGATGTTCCGTTAATTGCTTCCCTAGGTGCTGACGTTTGGGTGAAAATACCTTGTGAAAGTGAATCGTCATGTGAAAGTTCTCCCTCAAACATTTGTGTCATGATCAGAGTTGGAAACTGTCAGCTTAAGCCTGAGGATGATCACTTTTTTTATGGATTTGAGGGGCTAAATGATATCATAAATCAATTTTCTGTAGTTTCTGATGTATCTGAATGTTTCAAGTCTGATGTTCTACAATTTCTTCAGTCAAAAAGGTGCTTAGCACAGAAAAATGCAGTTCCACCTGTTGTTTCAAGCATTAATTATACTGAAGTTAGATGCTATATCAATTCATTGTTGATACAGTTGAATCCCTCCCAAAGAGTCTCGGAAGATCCTATTGCTACTGCTGAAATGAAATTCATCTGCTCTGCATCATTGAGAAATGACACTCTCTTAAGTGTAGCTCTAAGTTTTTCTTCCCTAGAAATATTTTCATTACCCAATTCTTTTGTATTAGCAAGATGCAAGTCCACAAGCTCAACCTCGTCAGTTCTTGAATTTTCTCTTACCAAAGAAAAAGATGGTGTAAATGAACTTCACGTTTCCTTGCCTTGTGTTGAAGTGTGGCTACATTTGTCTTACTGGAATGAGGTTGTTGACCTTTTCAAATCTTATGCTGGACAATCATCCACAAGTCTGCCGGACAACTTAGAGCAGGACACTGATGCCCTCATTGTGAGCTCAGAGAATATTTTCATTACATTTTACTTACCTGTTTGGACCGATGACCGAGCTTTTGGGGAATATCAGGGAGAGGAATGTCATGGAGATGGCTCTACAAATGGGTTATCTGACATAGTTGAAGCAAAAGCCTTTAGGTCCATTGCAGTTACTTTGTGCAGTAGAAGTAGTGAACTTTTTGTTGATGGAACAAATGTGAAAGTGAAATCCGACGTAGAAAAACTGGAGGGCATGGTATTGGTAAGCCAGGATGGAAGTGTTCAATCTTCACCCTTTTTCCAGATATCTCAGGTGCTTTTGGTAGCTGACATAAATAACCTTATGGAGCTTATGCATGTTGAAGTAGATGTTCAATTTGATCAATTAGATGTATGGATTTCGCACAGCATTCTCTACTTCTGGCATGGTGTACAATTCAGTGTTGCTGAAGGAGGGCCTTCTCAATTTTCTTTTGGTAGAATTGATGTTAAAGTCCAGCTTAGGAAGGTTTCTTTTCTGCTAAGCGATGGAAGGTGGAGTTGTAGTGGACCTCTGTTTCAGATTCTCTTGGGGAACATCCTCTTGCATATTACTGTGACTGAAAATAACCTCGAAGGTGCAGTTTCGGGTGACCTTCAAGTAAACTACAATAACATTCACAAGGTGTTCTGGGAGCCTTTTATTGAACCTTGGCAGTTTGAGGTCAATGTGATTAGAAAACAAGAGATGAGTCTCAGCAGCTCCAATTTCACAGATATCCATCTGAAATCAGCTGCACACCTTAATGTGAACTTCACTGAATCCCTTATAGAGTGTGTTTTCAGGACAGTTGAGATGATCAAGGATGCTTGGGTTCTAGTGTGTCCAAATGATCTTCCTGAAAGCCAAAAGTTATTAAATTCTCCGTTTGCTAAGCACACATATGCAGGAAAATATGCTCCTTATGTACTTCAAAACTTGACATCGCTGCCTCTAGCATATCGTGTTTATCAAGGCCCAATTAATTCTGATGAGTTGGATGTCTCAGAAACGAATAGAAAATATGTGCAACCAGGTTCTTCCATTCCAATATATATCGATGATACTCCTGAGAAACAACTTATTCATGTTAAGCCAGCACACTTCTCTGAGAGACTTTTTGACCAGAAGGCAAATGGGGTTCGCCATCAGTATATTACCATTCAACTTGATGGAACATCAGTGCCTTCTGAACCGATTTCAATGGATCTTGTAGGACTTACCTACTTTGAGGTTGATTTTTCTATGCCATACAGTGACAATATGGAAAACCATGGAACAAATGCCAATGCTGGTTTTGTTGTTCCTGTTGTCTTTGATGTTTCAGTGCAGCGGTACAGCAAATTAATCCGATTATACTCAACGGTTATACTTTCGAATTCAACTTCCATGCCATTGGAGCTGCGATTTGATATTCCATTTGGTGTGTCCCCAAAGATCTTAGATCCAATATATCCTGGTCAAGAGTTACCACTGCCTCTACACTTGGCCGAAGCTGGTAGGATTAGATGGCGTCCAATTGGCGATTCTCATCTCTGGAGTGAAGTTTATAACCTCTCAAACCTGCTTTTTCAGGAGGGCAAAATTGGATTTCTCAAGTCTTTTGCTTGCTATCCAGCCCATCCCAATAGTGATCCATTTCGCTGTTGTATCTCAGTTAGAAACGTCAGTTTACCTTCACCTGTAAGGTCAAGAAAGAGTTCTTTAAAGCAAACAGTTGCAAGTGGTGGTCAAATATTGCACAAGGACGAGGCAAAAAAGCAGTTTATTCACCAAGTAGTCCTCAGTATCCCTTTAGTAGTGAATAACTATCTTCCCGATGCAGTGACATTGACGATAGAAAGCGGTGGGATTACCCGGACTGCATTTCTTTCAGAGGTGGAAACTTCTTTTCATAATGTTGACCCTTCTCATCAGCTGAGATTGGAGATCCATATGCATGGCTTTAAAACAGCTATTTTGGATTTTCCACGTACTGAAATATTCTGTAAAAAGGCTAAGTTTGGTGGCACGAAGTTCTCACTTTCTGAAATTGTGACCTTTGACAGCGACTCAACAAATGGTCCAATGTATGTCACAGTGGAAAAGGTGATGGATGCATTTTCTGGAGCCAGGGAACTCTTTATCTCTGTTCCATTTCTGTTATATAATTGCACTGGCTTTCCGCTCTTCATTTCTGAATCTGCCAGTGAAATGAAAGGAGTAAGCTGCATTGTGCCTTCTTGTTATGATATGGTTGATCAAGAAGTACTTCAGGGAAAAAAGGATGGTCTTGGTCTGGTATCTTCTAATCATAATCCAAATGCTAGAGATTCAGATACAATGGGAAGTTCTTCCTCAAGAGTTCACATTGTCTCAATTAGAGACAATGCTACAACTCACAAAGAAATAAGTTTTAGCAAGCCTCTTTACTCTGAAGACAACTATCATGAGCTTTTAAGCAAACGTAATTTAGACAGGGAAAACTCACTTTCCAGCAGTTCACAGAATAGATCAAGTTCAAGCAGTCAGTTGACCTCAAAAGATTTAAACTCTAGTCGTTATGAGCGTGGAAGAGTAAGGGCATGCATGTTCTCTCCTAAACCAATTTCCTCTGCTGGTGAAGTAATGGTTAGAGTGAGTAGGTGTATGCCTGAATATGTAACGGAAAAGATGCCAAATTCTCTATGGTCAAGTTCATTTCCCCTTATTCCGCCTAGTGGTTCAACTACTGTCCTTGTTCCTCAACCATCGTCAAATGCGGCTTTTATGATGTCCATTACGTCCAGTGCAGTTGCCGCACCATTTGCTGGGAGGACAAGTGCAATTACCTTCCAACCTAGATACATCATCAGCAATGCATGCAGTAAGAACTTATGTTATAAGCAGAAAGGAACCGAATTTTCTTTTCAATTGGGTACAGGGGAACATTCTCATCTTCACTGGATGGATACAACCAGGGAGTTACTCGTTTCAATTCGTTATAATGAGCCTGGATGGCAATGGTCAGGTGGCTTCTTGCCAGACCATCTCGGTGACACTCAAGTGAAAATGCGGAATTATCTCTCTGGTTCCTTAAATATGATACGGGTTGAAGTGCAAAATGCTGACGTCTCCGTGGGAGATGAAAAGATTGTTGGAAACTTCTATGGAAATTCTGGGACAAACTTGATTCTTGTGTCAGATGATGAGACTGGGTATATGCCCTACAGGGTTGATAATTTTACTAATGAGAGGCTAAGGATCTACCAACAAAGATGTGAAACCTTTGAGACTATTGTTCAGTCTTACACTTCTTGCCCTTATGCCTGGGATGAACCATGCTATCCACATCGTCTCACTGTGGAGGTACCTGGTAAGCGTGTTTTAGGGTCATACGCTCTTGATGATGTAAAAGAATACAGTCCGGTGCAATTACCATCATCTGCTGAGAAACCTGAGAGAACATTGCATATATCCATCCATGTTGAGGGAGCAACAAAGGTCCTCCATGTTATTGATTCAAGTTACCATGTCATGAATGACAACAGAAGTATTAGTCTACCTCACTTAAAAGACAAAGGGAAGCATGAACAGAAACAGGACAAGTTTATTGGTTTCATGGAAAGAATTTCAGTTTTTATTCAACAGATTGGCATCTCTGTGATCAATATTCACCCTCAGGAGTTGCTTTTTATATGTGCAAAGAGCATTACAGTTGATCTGGTACAGAGTTTGGATCAACAAAAACTTTCCATCCAAATCGCATCATTGCAAGTCGATAATCAGCTTCGATCTTCCCCATATCCTGTTATGTTGTCTTTTGATCGTGAGTACAAAAACAATCCAGCTGGCCATGTGATTAGGGAGGATGATGTTAAACCAAGTGAAAGGATTTTGCAAAGGACTTCTCGCAGTTTTGAACCTGTATTCTGCCTAGCTGTATCTAAGTGGAGGAAGAAAGATGTCTCACTGGTTTCGTTTGAATACATAAGCTTAAGAGTTGCAGATGTTTGTCTTGAGCTTGAACAAGAATTGATATTAAGCCTATTTGGCTTCATAAGAAATGTATCTTCAAGGTTCCAGAGTAGAGTCTTGCCATTATCAGATCCGTTCCTGCACCATCCTTCTAATGATGCTGGCTCAATGGATGCGTATGCAACTGATAACCAACTTCACTTAATGAGTGTTCCTCTGCTTAATGAAAACCACAAGCAAAGACTCTCACTACCTTCAATAGTTCCAATTGGAGCTCCTTGGCAGCAAATTTACCTTTTAGCTAGAAGACAGAAAAAGATATATGTAGAAGTGTTTGAATTTTCCCCCATCAAGTTGACCCTAAGCTTTTCCAGCACTCCATGGATGCTTAGAAATGGGATTCTTGCAGCAGGAGAATCTGTTATTCATAGAGGTCTGATGGCTCTTGCTGATGTGGAGGGTGCACGAATTCATCTGAAGCAGTTGACAATTGCACATCAAATTGCTAGCTTGGAGTCTCTACAAGAGATCCTTATGAGGCATTATACTCGGCAACTTCTTCATGAGATGTATAAGGTTTTTGGTTCAGCTGGTGTAATTGGCAATCCCATGGGATTTGCAAGGAGTTTAGGGCTTGGCATTAGAGACTTCTTGTCAGTGCCTGCCAGAAGCATTTTTCAGAGCCCTACTGGATTAATTACTGGCATGGCACAAGGCACGACTAGTCTCCTAAGTAATACAGTCTATGCTATAAGTGATGCTGCCACCCAATTCAGCAAAGCTGCGCACAAGGGAATTGTGGCATTTACATTTGATGACCAGGCTGTTTCAGGGGTGCAACAGCAACAGACTGGTATAGCATCTCACTCTAAAGGTGTTATCAATGAAGTATTTGAGGGACTCACAGGTCTTCTTCAATCACCAATCAATGGAGCTGAGAAACATGGTCTTCCTGGTGTGCTCTCAGGCATAGCTTTAGGTATTACAGGACTTGTGGCCAAACCAGCTGCTAGTATTCTTGAAGTTACAGGAAAAACTGCACAGAGCATTAGAAACCGAAGTAGGATCTATCAGACAGGACAACAGCGCTTTAGAGTCCGTCTTCCAAGGCCTCTAAGCCGAGAATATCCCTTAAGACCTTACTGTTGGGAAGAAGCTGTTGGAGCAGCAGTACTTGTTGAGGCAGATGATAGTTTGAGGCTCAAGGATGAAATTTTTGTTATGTGCAAGAAACTTAAAGAAGCTGGTAAATTTGTCATCATCACAGAGAGACTGGTTTTGATTGTAAGCTGCTCAAGTTTGGTGGACTTGAGTAAGCCTGAATTTCGTGGTGTTCCTTCTGATCTCAAATGGGTGATTGAATCAGAAATTCATTTGGAGAGTGCGATGCATGCTGACTGCGATCAAGGAGTAGTTCATATAGTCGGCAGCAGCTCTAATACGCCTTTGCGTCAAAACCAGCTAGCCAAAAGAAGCAGTGGAACAAGGGCGGTGCGTTGGAATAATCCTACTGTTCCCCTCATTCAGACCAACTTGGAACTAGAACACAAAGAAGATGCGGAGAACTTGTTACAGGTTTTATTGTCTACAATTGAACTGGGAAAAGACCAAGGTTGGGGTTGTAGGAATATTCTGCACCGAAGTAATATCAAATAG

Protein Analysis

3116

Amino Acids

348.31

Weight (kDa)

5.83

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_N PF12624 3 - 406 2.9e-21 VPS13-like family N-terminal region
VPS13_M PF25033 1431 - 1526 2.1e-08 VPS13-like family middle region
VPS13_VAB PF25036 1693 - 1983 5.4e-09 Vacuolar-sorting associated protein 13, adaptor binding domain
VPS13_VAB PF25036 2090 - 2354 8.2e-24 Vacuolar-sorting associated protein 13, adaptor binding domain
VPS13_C PF25037 2931 - 3000 9.9e-09 Intermembrane lipid transfer protein VPS13, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 5310, 6621, 6723
AasI GACNNNNNNGTC 2 cut(s) 2729, 8601
AatII GACGTC 1 cut(s) 6852
AbsI CCTCGAGG 1 cut(s) 749
Acc16I TGCGCA 3 cut(s) 21, 478, 8481
Acc36I ACCTGC 1 cut(s) 5331
Acc65I GGTACC 1 cut(s) 7087
AccB1I GGYRCC 1 cut(s) 7087
AccB7I CCANNNNNTGG 3 cut(s) 1609, 6548, 8624
AccBSI CCGCTC 1 cut(s) 5926
AccI GTMKAC 3 cut(s) 913, 7279, 9281
AccII CGCG 1 cut(s) 2526
AcoI YGGCCR 5 cut(s) 532, 2670, 5247, 7570, 8691
AcuI CTGAAG 9 cut(s) 1122, 2421, 2843, 3266, 3369, 4209, 5996, 6195, 8192
AcyI GRCGYC 2 cut(s) 5272, 6849
AdeI CACNNNGTG 1 cut(s) 4405
AfeI AGCGCT 1 cut(s) 8783
AflII CTTAAG 4 cut(s) 3164, 3475, 7714, 8827
AflIII ACRYGT 1 cut(s) 2578
AhdI GACNNNNNGTC 1 cut(s) 8347
AhlI ACTAGT 2 cut(s) 2333, 8417
AjuI GAANNNNNNNTTGG 8 cut(s) 2610, 2642, 5269, 5301, 6858, 6890, 8838, 8870
AleI CACNNNNGTG 2 cut(s) 614, 6782
Alw21I GWGCWC 6 cut(s) 1426, 1997, 3764, 7972, 8161, 8658
Alw44I GTGCAC 1 cut(s) 8157
AlwNI CAGNNNCTG 1 cut(s) 8357
Ama87I CYCGRG 2 cut(s) 749, 2183
Aor51HI AGCGCT 1 cut(s) 8783
ApaI GGGCCC 1 cut(s) 2543
ApaLI GTGCAC 1 cut(s) 8157
ArsI GACNNNNNNTTYG 2 cut(s) 3800, 3832
AseI ATTAAT 5 cut(s) 2444, 3339, 4743, 5105, 8390
Asp700I GAANNNNTTC 5 cut(s) 1127, 3773, 5458, 6009, 6091
Asp718I GGTACC 1 cut(s) 7087
AspA2I CCTAGG 2 cut(s) 2423, 3061
AspLEI GCGC 4 cut(s) 22, 479, 8482, 8784
AsuC2I CCSGG 1 cut(s) 5617
AsuII TTCGAA 1 cut(s) 5135
AsuNHI GCTAGC 2 cut(s) 8201, 9157
AvaI CYCGRG 2 cut(s) 749, 2183
AvaII GGWCC 9 cut(s) 874, 2079, 2427, 2932, 3801, 3900, 4289, 5822, 7222
AvrII CCTAGG 2 cut(s) 2423, 3061
AxyI CCTNAGG 2 cut(s) 3171, 7398
BaeGI GKGCMC 3 cut(s) 2543, 2820, 8161
BalI TGGCCA 4 cut(s) 534, 2672, 7572, 8693
BanI GGYRCC 1 cut(s) 7087
BanII GRGCYC 5 cut(s) 1997, 2543, 3764, 7972, 8381
BarI GAAGNNNNNNTAC 8 cut(s) 1344, 1376, 1663, 1695, 3600, 3632, 3633, 3665
BbsI GAAGAC 5 cut(s) 6183, 8008, 8597, 8636, 8789
Bbv12I GWGCWC 6 cut(s) 1426, 1997, 3764, 7972, 8161, 8658
BcgI CGANNNNNNTGC 2 cut(s) 8225, 8259
BciVI GTATCC 3 cut(s) 2025, 5558, 6682
BclI TGATCA 8 cut(s) 151, 1786, 3141, 3178, 4120, 4578, 5998, 7383
BcnI CCSGG 1 cut(s) 5617
BcuI ACTAGT 2 cut(s) 2333, 8417
BfmI CTRYAG 5 cut(s) 240, 597, 718, 3231, 6946
BfoI RGCGCY 1 cut(s) 8785
BfrI CTTAAG 4 cut(s) 3164, 3475, 7714, 8827
BfuAI ACCTGC 1 cut(s) 5331
BfuI GTATCC 3 cut(s) 2025, 5558, 6682
BglI GCCNNNNNGGC 2 cut(s) 6736, 7770
BglII AGATCT 2 cut(s) 1180, 5196
BlnI CCTAGG 2 cut(s) 2423, 3061
BlpI GCTNAGC 3 cut(s) 3298, 4262, 4653
BmcAI AGTACT 3 cut(s) 172, 6009, 8868
Bme18I GGWCC 9 cut(s) 874, 2079, 2427, 2932, 3801, 3900, 4289, 5822, 7222
BmeRI GACNNNNNGTC 1 cut(s) 8347
BmeT110I CYCGRG 2 cut(s) 749, 2183
BmrI ACTGGG 3 cut(s) 2085, 6942, 9302
BmtI GCTAGC 2 cut(s) 8205, 9161
BmuI ACTGGG 3 cut(s) 2085, 6942, 9302
BoxI GACNNNNGTC 2 cut(s) 351, 919
BpiI GAAGAC 5 cut(s) 6183, 8008, 8597, 8636, 8789
BplI GAGNNNNNCTC 2 cut(s) 7894, 7926
BpmI CTGGAG 5 cut(s) 354, 1553, 4007, 5317, 5886
Bpu10I CCTNAGC 3 cut(s) 4241, 6977, 8057
Bpu1102I GCTNAGC 3 cut(s) 3298, 4262, 4653
Bpu14I TTCGAA 1 cut(s) 5135
BpuEI CTTGAG 7 cut(s) 5339, 6082, 6765, 7757, 8882, 8974, 9025
BpuMI CCSGG 1 cut(s) 5617
Bsa29I ATCGAT 1 cut(s) 4818
BsaAI YACGTR 2 cut(s) 2579, 5732
BsaBI GATNNNNATC 4 cut(s) 2358, 4500, 5289, 7373
BsaHI GRCGYC 2 cut(s) 5272, 6849
BsaI GGTCTC 1 cut(s) 2046
BsaWI WCCGGW 1 cut(s) 7144
Bse21I CCTNAGG 2 cut(s) 3171, 7398
Bse3DI GCAATG 6 cut(s) 1401, 3903, 6601, 6746, 7184, 7487
Bse8I GATNNNNATC 4 cut(s) 2358, 4500, 5289, 7373
BseCI ATCGAT 1 cut(s) 4818
BseJI GATNNNNATC 4 cut(s) 2358, 4500, 5289, 7373
BseMI GCAATG 6 cut(s) 1401, 3903, 6601, 6746, 7184, 7487
BseSI GKGCMC 3 cut(s) 2543, 2820, 8161
BseYI CCCAGC 3 cut(s) 1399, 2272, 6548
BsgI GTGCAG 8 cut(s) 3940, 4380, 4500, 5108, 6549, 7820, 8718, 9314
Bsh1236I CGCG 1 cut(s) 2526
BshNI GGYRCC 1 cut(s) 7087
BshVI ATCGAT 1 cut(s) 4818
BsiHKAI GWGCWC 6 cut(s) 1426, 1997, 3764, 7972, 8161, 8658
BsiHKCI CYCGRG 2 cut(s) 749, 2183
BsiSI CCGG 4 cut(s) 1924, 3722, 5617, 7145
BslFI GGGAC 7 cut(s) 2166, 2225, 2422, 2918, 5173, 6913, 8607
BsmBI CGTCTC 2 cut(s) 6856, 7079
BsmFI GGGAC 7 cut(s) 2166, 2225, 2422, 2918, 5173, 6913, 8607
BsmI GAATGC 3 cut(s) 1649, 1750, 4149
Bso31I GGTCTC 1 cut(s) 2046
BsoBI CYCGRG 2 cut(s) 749, 2183
Bsp119I TTCGAA 1 cut(s) 5135
Bsp120I GGGCCC 1 cut(s) 2539
Bsp1286I GDGCHC 9 cut(s) 1426, 1997, 2543, 2820, 3764, 7972, 8161, 8381, 8658
Bsp1407I TGTACA 2 cut(s) 2873, 4171
Bsp1720I GCTNAGC 3 cut(s) 3298, 4262, 4653
Bsp19I CCATGG 4 cut(s) 2736, 5032, 8075, 8304
BspDI ATCGAT 1 cut(s) 4818
BspFNI CGCG 1 cut(s) 2526
BspHI TCATGA 5 cut(s) 2386, 3138, 6187, 7254, 8257
BspMAI CTGCAG 2 cut(s) 244, 601
BspMI ACCTGC 1 cut(s) 5331
BspOI GCTAGC 2 cut(s) 8205, 9161
BspQI GCTCTTC 1 cut(s) 5933
BspT104I TTCGAA 1 cut(s) 5135
BspT107I GGYRCC 1 cut(s) 7087
BspTI CTTAAG 4 cut(s) 3164, 3475, 7714, 8827
BspTNI GGTCTC 1 cut(s) 2046
BsrBI CCGCTC 1 cut(s) 5926
BsrDI GCAATG 6 cut(s) 1401, 3903, 6601, 6746, 7184, 7487
BsrGI TGTACA 2 cut(s) 2873, 4171
BssNI GRCGYC 2 cut(s) 5272, 6849
Bst6I CTCTTC 6 cut(s) 44, 280, 1059, 2440, 5933, 6307
BstACI GRCGYC 2 cut(s) 5272, 6849
BstAFI CTTAAG 4 cut(s) 3164, 3475, 7714, 8827
BstAPI GCANNNNNTGC 2 cut(s) 6545, 6600
BstAUI TGTACA 2 cut(s) 2873, 4171
BstBAI YACGTR 2 cut(s) 2579, 5732
BstBI TTCGAA 1 cut(s) 5135
BstDSI CCRYGG 7 cut(s) 292, 2736, 2820, 5032, 6855, 8075, 8304
BstEII GGTNACC 2 cut(s) 2150, 4370
BstFNI CGCG 1 cut(s) 2526
BstH2I RGCGCY 1 cut(s) 8785
BstHHI GCGC 4 cut(s) 22, 479, 8482, 8784
BstNSI RCATGY 8 cut(s) 1824, 2615, 2642, 4100, 6327, 6331, 6603, 9089
BstPAI GACNNNNGTC 2 cut(s) 351, 919
BstPI GGTNACC 2 cut(s) 2150, 4370
BstSFI CTRYAG 5 cut(s) 240, 597, 718, 3231, 6946
BstSLI GKGCMC 3 cut(s) 2543, 2820, 8161
BstUI CGCG 1 cut(s) 2526
BstV2I GAAGAC 5 cut(s) 6183, 8008, 8597, 8636, 8789
BstXI CCANNNNNNTGG 2 cut(s) 5155, 5699
Bsu15I ATCGAT 1 cut(s) 4818
Bsu36I CCTNAGG 2 cut(s) 3171, 7398
BsuI GTATCC 3 cut(s) 2025, 5558, 6682
BsuTUI ATCGAT 1 cut(s) 4818
BtgI CCRYGG 7 cut(s) 292, 2736, 2820, 5032, 6855, 8075, 8304
BtgZI GCGATG 3 cut(s) 4281, 4689, 9095
BtsI GCAGTG 5 cut(s) 512, 1903, 5231, 5589, 9178
BveI ACCTGC 1 cut(s) 5331
CaiI CAGNNNCTG 1 cut(s) 8357
CciI TCATGA 5 cut(s) 2386, 3138, 6187, 7254, 8257
CfoI GCGC 4 cut(s) 22, 479, 8482, 8784
ClaI ATCGAT 1 cut(s) 4818
CseI GACGC 3 cut(s) 1156, 5261, 9134
CsiI ACCWGGT 2 cut(s) 4792, 7090
CspCI CAANNNNNGTGG 6 cut(s) 1014, 1049, 2347, 2382, 8979, 9014
DraI TTTAAA 6 cut(s) 846, 2571, 2970, 5466, 5707, 6288
DraIII CACNNNGTG 1 cut(s) 4405
DrdI GACNNNNNNGTC 2 cut(s) 2729, 8601
DriI GACNNNNNGTC 1 cut(s) 8347
DseDI GACNNNNNNGTC 2 cut(s) 2729, 8601
EaeI YGGCCR 5 cut(s) 532, 2670, 5247, 7570, 8691
Eam1104I CTCTTC 6 cut(s) 44, 280, 1059, 2440, 5933, 6307
Eam1105I GACNNNNNGTC 1 cut(s) 8347
EarI CTCTTC 6 cut(s) 44, 280, 1059, 2440, 5933, 6307
EciI GGCGGA 1 cut(s) 6441
Ecl136II GAGCTC 3 cut(s) 1995, 3762, 7970
Eco147I AGGCCT 2 cut(s) 676, 8806
Eco24I GRGCYC 5 cut(s) 1997, 2543, 3764, 7972, 8381
Eco31I GGTCTC 1 cut(s) 2046
Eco32I GATATC 3 cut(s) 3214, 4047, 4498
Eco47I GGWCC 9 cut(s) 874, 2079, 2427, 2932, 3801, 3900, 4289, 5822, 7222
Eco47III AGCGCT 1 cut(s) 8783
Eco53kI GAGCTC 3 cut(s) 1995, 3762, 7970
Eco57I CTGAAG 9 cut(s) 1122, 2421, 2843, 3266, 3369, 4209, 5996, 6195, 8192
Eco81I CCTNAGG 2 cut(s) 3171, 7398
Eco88I CYCGRG 2 cut(s) 749, 2183
Eco91I GGTNACC 2 cut(s) 2150, 4370
EcoICRI GAGCTC 3 cut(s) 1995, 3762, 7970
EcoO109I RGGNCCY 7 cut(s) 874, 2079, 2539, 2540, 2932, 4196, 7222
EcoO65I GGTNACC 2 cut(s) 2150, 4370
EcoRI GAATTC 2 cut(s) 5137, 8163
EcoRV GATATC 3 cut(s) 3214, 4047, 4498
EcoT22I ATGCAT 7 cut(s) 2402, 4098, 5700, 5860, 6329, 6601, 9087
EcoT38I GRGCYC 5 cut(s) 1997, 2543, 3764, 7972, 8381
Esp3I CGTCTC 2 cut(s) 6856, 7079
FaqI GGGAC 7 cut(s) 2166, 2225, 2422, 2918, 5173, 6913, 8607
FauNDI CATATG 3 cut(s) 1915, 4663, 5694
FbaI TGATCA 8 cut(s) 151, 1786, 3141, 3178, 4120, 4578, 5998, 7383
FblI GTMKAC 3 cut(s) 913, 7279, 9281
FriOI GRGCYC 5 cut(s) 1997, 2543, 3764, 7972, 8381
FspAI RTGCGCAY 1 cut(s) 478
FspI TGCGCA 3 cut(s) 21, 478, 8481
GlaI GCGC 4 cut(s) 21, 478, 8481, 8783
GsaI CCCAGC 3 cut(s) 1403, 2276, 6552
GsuI CTGGAG 5 cut(s) 354, 1553, 4007, 5317, 5886
HaeII RGCGCY 1 cut(s) 8785
HapII CCGG 4 cut(s) 1924, 3722, 5617, 7145
HgaI GACGC 3 cut(s) 1156, 5261, 9134
HhaI GCGC 4 cut(s) 22, 479, 8482, 8784
Hin1I GRCGYC 2 cut(s) 5272, 6849
Hin6I GCGC 4 cut(s) 20, 477, 8480, 8782
HinP1I GCGC 4 cut(s) 20, 477, 8480, 8782
HincII GTYRAC 7 cut(s) 115, 914, 3679, 5662, 6273, 8052, 8181
HindII GTYRAC 7 cut(s) 115, 914, 3679, 5662, 6273, 8052, 8181
HindIII AAGCTT 3 cut(s) 2301, 7711, 8059
HpaI GTTAAC 1 cut(s) 115
HpaII CCGG 4 cut(s) 1924, 3722, 5617, 7145
Hpy99I CGWCG 1 cut(s) 3977
HpyCH4IV ACGT 9 cut(s) 2578, 3072, 3621, 3975, 5424, 5731, 6205, 6521, 6849
HpySE526I ACGT 9 cut(s) 2578, 3072, 3621, 3975, 5424, 5731, 6205, 6521, 6849
Hsp92I GRCGYC 2 cut(s) 5272, 6849
HspAI GCGC 4 cut(s) 20, 477, 8480, 8782
KflI GGGWCCC 1 cut(s) 2932
KpnI GGTACC 1 cut(s) 7091
Ksp22I TGATCA 8 cut(s) 151, 1786, 3141, 3178, 4120, 4578, 5998, 7383
KspAI GTTAAC 1 cut(s) 115
LguI GCTCTTC 1 cut(s) 5933
MabI ACCWGGT 2 cut(s) 4792, 7090
MaeII ACGT 9 cut(s) 2578, 3072, 3621, 3975, 5424, 5731, 6205, 6521, 6849
MbiI CCGCTC 1 cut(s) 5926
MfeI CAATTG 7 cut(s) 159, 1607, 5277, 6650, 7962, 8184, 9285
MhlI GDGCHC 9 cut(s) 1426, 1997, 2543, 2820, 3764, 7972, 8161, 8381, 8658
MlsI TGGCCA 4 cut(s) 534, 2672, 7572, 8693
MluNI TGGCCA 4 cut(s) 534, 2672, 7572, 8693
Mox20I TGGCCA 4 cut(s) 534, 2672, 7572, 8693
Mph1103I ATGCAT 7 cut(s) 2402, 4098, 5700, 5860, 6329, 6601, 9087
MroXI GAANNNNTTC 5 cut(s) 1127, 3773, 5458, 6009, 6091
MscI TGGCCA 4 cut(s) 534, 2672, 7572, 8693
Msp20I TGGCCA 4 cut(s) 534, 2672, 7572, 8693
MspA1I CMGCKG 7 cut(s) 1149, 4514, 5092, 5677, 7568, 8285, 8702
MspCI CTTAAG 4 cut(s) 3164, 3475, 7714, 8827
MspI CCGG 4 cut(s) 1924, 3722, 5617, 7145
MunI CAATTG 7 cut(s) 159, 1607, 5277, 6650, 7962, 8184, 9285
Mva1269I GAATGC 3 cut(s) 1649, 1750, 4149
MvnI CGCG 1 cut(s) 2526
NciI CCSGG 1 cut(s) 5617
NcoI CCATGG 4 cut(s) 2736, 5032, 8075, 8304
NdeI CATATG 3 cut(s) 1915, 4663, 5694
NheI GCTAGC 2 cut(s) 8201, 9157
NmeAIII GCCGAG 2 cut(s) 8224, 8840
NsbI TGCGCA 3 cut(s) 21, 478, 8481
NsiI ATGCAT 7 cut(s) 2402, 4098, 5700, 5860, 6329, 6601, 9087
NspI RCATGY 8 cut(s) 1824, 2615, 2642, 4100, 6327, 6331, 6603, 9089
NspV TTCGAA 1 cut(s) 5135
OliI CACNNNNGTG 2 cut(s) 614, 6782
PaeI GCATGC 4 cut(s) 1824, 6327, 6603, 9089
PaeR7I CTCGAG 1 cut(s) 749
PagI TCATGA 5 cut(s) 2386, 3138, 6187, 7254, 8257
PceI AGGCCT 2 cut(s) 676, 8806
PciSI GCTCTTC 1 cut(s) 5933
PcsI WCGNNNNNNNCGW 1 cut(s) 6304
PctI GAATGC 3 cut(s) 1649, 1750, 4149
PdmI GAANNNNTTC 5 cut(s) 1127, 3773, 5458, 6009, 6091
PflMI CCANNNNNTGG 3 cut(s) 1609, 6548, 8624
Ppu21I YACGTR 2 cut(s) 2579, 5732
PpuMI RGGWCCY 4 cut(s) 874, 2079, 2932, 7222
PshAI GACNNNNGTC 2 cut(s) 351, 919
PshBI ATTAAT 5 cut(s) 2444, 3339, 4743, 5105, 8390
PsiI TTATAA 3 cut(s) 5310, 6621, 6723
Psp124BI GAGCTC 3 cut(s) 1997, 3764, 7972
Psp5II RGGWCCY 4 cut(s) 874, 2079, 2932, 7222
PspEI GGTNACC 2 cut(s) 2150, 4370
PspFI CCCAGC 3 cut(s) 1399, 2272, 6548
PspOMI GGGCCC 1 cut(s) 2539
PspPPI RGGWCCY 4 cut(s) 874, 2079, 2932, 7222
PspXI VCTCGAGB 1 cut(s) 749
PstI CTGCAG 2 cut(s) 244, 601
PstNI CAGNNNCTG 1 cut(s) 8357
PvuII CAGCTG 6 cut(s) 1149, 4514, 5677, 7568, 8285, 8702
SacI GAGCTC 3 cut(s) 1997, 3764, 7972
SalI GTCGAC 1 cut(s) 912
SapI GCTCTTC 1 cut(s) 5933
ScaI AGTACT 3 cut(s) 172, 6009, 8868
SduI GDGCHC 9 cut(s) 1426, 1997, 2543, 2820, 3764, 7972, 8161, 8381, 8658
SexAI ACCWGGT 2 cut(s) 4792, 7090
SfcI CTRYAG 5 cut(s) 240, 597, 718, 3231, 6946
Sfr274I CTCGAG 1 cut(s) 749
SfuI TTCGAA 1 cut(s) 5135
SinI GGWCC 9 cut(s) 874, 2079, 2427, 2932, 3801, 3900, 4289, 5822, 7222
SlaI CTCGAG 1 cut(s) 749
SpeI ACTAGT 2 cut(s) 2333, 8417
SphI GCATGC 4 cut(s) 1824, 6327, 6603, 9089
SseBI AGGCCT 2 cut(s) 676, 8806
SspI AATATT 8 cut(s) 1156, 1323, 3510, 3772, 5496, 5742, 7390, 9325
SstI GAGCTC 3 cut(s) 1997, 3764, 7972
StuI AGGCCT 2 cut(s) 676, 8806
TaiI ACGT 9 cut(s) 2581, 3075, 3624, 3978, 5427, 5734, 6208, 6524, 6852
TaqII GACCGA 3 cut(s) 2536, 3818, 3825
TatI WGTACW 7 cut(s) 170, 2873, 4171, 4687, 6007, 7551, 8866
TauI GCSGC 2 cut(s) 6503, 6539
TspGWI ACGGA 8 cut(s) 61, 587, 3036, 4635, 6419, 6844, 7818, 8783
Van91I CCANNNNNTGG 3 cut(s) 1609, 6548, 8624
Vha464I CTTAAG 4 cut(s) 3164, 3475, 7714, 8827
VneI GTGCAC 1 cut(s) 8157
VpaK11BI GGWCC 9 cut(s) 874, 2079, 2427, 2932, 3801, 3900, 4289, 5822, 7222
VspI ATTAAT 5 cut(s) 2444, 3339, 4743, 5105, 8390
XceI RCATGY 8 cut(s) 1824, 2615, 2642, 4100, 6327, 6331, 6603, 9089
XcmI CCANNNNNNNNNTGG 4 cut(s) 1528, 1684, 4892, 6423
XhoI CTCGAG 1 cut(s) 749
XmaJI CCTAGG 2 cut(s) 2423, 3061
XmiI GTMKAC 3 cut(s) 913, 7279, 9281
XmnI GAANNNNTTC 5 cut(s) 1127, 3773, 5458, 6009, 6091
ZraI GACGTC 1 cut(s) 6850
ZrmI AGTACT 3 cut(s) 172, 6009, 8868
Zsp2I ATGCAT 7 cut(s) 2402, 4098, 5700, 5860, 6329, 6601, 9087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.