Rh2AG464200

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001657
Physical Location & Seq
Forward (+)
68194200 .. 68223024
28825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG464200.1

Sequence Viewer

Length: 9717 bp
ATGGGTTTGGAGTTTTACTGTGGCACACTTGATGGGGCGGTGGAGTTGATGGCTATGAACAATGATGGGGATTCCGTAGTATGGCAAGATATAAGATATGACGGGAAAAGATATAACAACTCCATACTGTCACCATGTGATGTTTCCATGTCACTCTCGGTCAACAGATCAGGAGAGCTTGATAGCAAAATCCCGCAGTACTCCATCACCGCTGAGATAACTGACTTGGTGATGTGCTTAGATGAGGTTCAGTTGCAACAGATTTTGTTTCTGTGGGATTATTTTTGTACATGTGAATTGAGAAATAAGTATGGGCGTTATCGTCCCTGGAGTAGTCCGTTATCAAAAAAGGTGAAGGGTTGGCAAATGTTATGGTGGCATTATGCGCAAGAATCTATCTTATCAGATGTACGGAAGAGATTAAAGAAAACATCGTGGAGACACTTTGGACAGAGACTAAGCTCTTGTCGGAAGTATGTTAACCTATATAAGACTAAGTTGGATTTTCTTCGCCATGATCAGCCAATTGATGAAAGTACTCGATGGGAATTGGAGCAGATGGAGAAAGAATTAGACATAGATGATATTTTAAGTTACAGGTCGGCTGCAGAATGTGAGTTGCAGGAGTTTTTGGTGAACTCTTCAACTGGAAAATCCCGTGGATGGCTGAACTGGCTATCTCTTGGTATGCTTGGTGCTGGAGGAACAGATGACTTGGGTCAATTTTCTGGGGCTGTTTTTGTTTCTGATGCAGTTATTAAGGATATATATGAGGCGACAAAGTTCAATCCTCCTATTTTATCCAATGGGGATGCTCGAACAACTGATAAGATTGATATATGCGCACTCCATTTCAGCATCAATCGGATTTCTGCAACACTGCGTAACATGAAGTATGGCCAAGAAATTGCAGAGTTGATGTTGAATGGAGTGACTACGGAGTGCAAATTTAGGGAAGAATCTGCAGCCATTGTCACCATTGTGAACTCAGGGCAGATGGTTTATCCTTGTAACAAGAAAGTCATTCTGCATTTGCGAAGGCCTAATAATGAGAAGAACGAACTGGAAAACGAGAATCCTTCCTGTAGATTGCAAGTGGATGTATCATCAAACCTCGAGGCTGAATTGTCCATTAAGGGGATGCTTCATCCATTAGAAGTCACCATTGATGCAGAATTTTTCTTAAAGTTGATGGATTTTCTTGGAGCTTTAAAATCCTTTGAAAGTCAGCATGGAAGGGTCCTTCTGTCGCTAAATGGGATAGAAAATGTCAATGGTCGACTGTTGTCGAAAGCTGAATACCTTTTATCACATCATAGGAAAGTGGTGTGGGATGTTACTATCTTCAATATCATCATAAATGTTCCTTGGAGAGAGGCAATAACCGATCCACATAACTTGGTTTTTGAAGCAGGCTCTCTTCTTTTTAGGACTAAATGTGATATGGGATCTCAGCCATCAGATGATGAGGAACAATTTTATACTCTGAAAAAGTTCTTAACTTCCGTTTCCAGCTGTAATATTTCTACGTGCGTCCAAATTCAAGATCTGTACGATCACTATGAGGTCAAGTTAAATGATTTTGAGTTGAAGGTAACGATGCCTTCTCATTCTTCTCCAATTTCTATCTTGGAGAAACTTAGTCCTTCTGTCTCCTTTGCATTTTGCTTGATCCAAGATGAGTCAATATTGAAACAGTTGGAGGCTTGTGTCATTGTATCATCACTTCATGCACATTTTTCTCCATCGATATATGCTGCAATGCTGGGATTGATAGAATATCTAGGTGCTCTGCAATTGAATTTTGATTCTCTACCCTTGGGAACTATTGATTCACTTGATGCCACATCAAATGGATTAGGGAATCCTGTTTTCGGTTTCTCTACCAATATCAAACTGGAGACAGTGAAGATTGAAGTCGAACTGGAAAATGAAGAAGAAAATAGCTCGTCATTCATGCTTAAATTCCAACAATTGGACATGGGTTATTCGCTTTCAAAAATTGAGGAATGCTGGATCTTAATGAACGCATTGAGTATCACGACTTCTAAATTGGCTGATAAAAGTGATAGTCGGATTTTGTATTCATCTGGAAATCAATCATCTATGAATGCTTTACATCCACATGAGATTGGGATTGGCAATAGAAATGATCATTTTGCCAACACCATTGGAAATAATGAAGCTTGCTTTACTTTACAATATGAATCTCATCCGAAGGAGACTGTTCCTCAAAAATGTAGAATATGCTTGAATAACGGTGATCTCCACTGCTACCCATATGTTATCCGGCTTCTGGTTGCATTTTTTGATAAACTTTCTACTTATGGTGCATCTAATCCTGGTAAGAACACTTTGAGCTCATCTGTGGATGCTAGGTATCCAAAAAGTGTTCCTGGTTTTGGGTCTCAAAGGTTTGGTTTCTCCAACTTTGTAGAAACTGGGTCCTCTGAGTATGCAAGCATACCTTTAGATCATTTTCCTTTTGTTATGCTATCCAATGCTGCTTCCCTTGGTAACCTTGAAAGTTCTTTGCTTTATGCCAGTCCCGAGTGGAGAAAGTATTTCAGTATAAGGGACAGCAGAATCAGCAGCCCACTATCCAGCATAAAGGAGGTATCTAAAAAGTTCCATGCCCCAGCATTGAAGCACTCATCTGTGGTTGAAGCTTCTGCTGTGTGTGGGAGTTCTGCCACTACTAGTAGTCCGCTTGTAATTGATGTCAATCTCTGTGGAATCAGAGTGCATTTTCATGATTCAAAATGCATTGTTGGGACGATTACCCTGCCTAGGTCCAACTCTTCAGTATTAATTTCTGAAAACAGCTTTGATATATTGTGTTCTACTGAGGGGTTAGTTCTCAGTTCATCTTGGTCGTCACAGAATTTACGCGAGTTTCTGTGGGGCCCCTCGGTCTCGAATTTATCTCCTATTTTAAATGTACGTGTGAGGAAAGAATGTGGGCCATTGAGTTCGCATGTTGAACTATGCTTTGGCATACAGCATGTATATTGCATCTTACCTCCTGAATATCTGGCCATAATAATTGGTTACTTTTCATTGTCTGATTGGAGTTCAGATTCTAATGACCAGCTTGTCACCATGGGGCATGAAGATACTGAAAGTGAAAGTGAATGTTCCTTTATCTACAAGATTGAGATACTGGACTCTGTTTTAGTTGTGCCCGTGGAAAGCAACGAGGGTCAGTTTCTAAAGAGTGAACTTCAGCAGTTCTACTGTACATTTATTCAAAGCAGTTCAAATAATGTATTGAAGGACATTCCCCATGAATGTTGGGTCCCGACTGATAAACTTGCTAAAAGAAATCATTGTTTAAATTTATTTGGACGAGACTTGTTTCTATCATTCCTGTCTTTCAAGGACAATCAATCCAATTCCTTTGTAGATGTTCCGTTAATTGCTTCCCTAGGTGCTGACGTTTGGGTGAAAATACCTTGTGAAAGTGAATCGTCATTTGAAAGTTCTCCCTCAAACATTTGTGTCATGATCAGAGTTGGAAACTGTCAGCTTAAGCCTGAGGATGATCACTTTTTTTATGGATTTCAGGGGCTAAATGATATCATAAATCAATTTTCTGTAGTTTCTGATGTATCTGAATGTTTCAAGTCTGATGTTCTACAATTTCTTCAGTCAAAAAGGTGCTTAGCACAGAAAAATGCAGTTCCACCTGTTGTTTCAAGCATTAATTATACCGAAGTTAGATGCTATATCAATTCATTGTTGATACAGTTGAATCCCTGCCAAAGAGTCTCAGAAGATCCTATTGCTACTGCTGAAATGAAATTCATCTGCTCTGCATCATTGAGAAATGACACTCTCTTAAGTGTAGCTCTAAGTTTTTCTTCCCTAGAAATGTGTTCATTACCTAATTCTTTTGTATTAGCAAGATGCAAGTCCACAAGCTCAACCTCGTCAGTTCTTGAATTTTCTCTTACCAAAGAAAAAGATGGTGTAAATGAACTTCACGTTTCCTTGCCTTGTGTTGAAGTGTGGTTACATTTGTCTTACTGGAATGAGGTTGTTGACCTTTTCAAATCTTATGCTGGACAATCATCCACAAGTCTGCCGGACAACTTAGAGCAGGACACTGATGCCCTCATTGTGAGCTCGGAGAATATATTCATTACATTTTACTTACCTGTTTGGACTGATGACCGAGCTTTTGGGGAATATCAGGGAGAGGAATGTCATGTAGATGGCTCTACAAATGGGTTATCTGACATAGTTGAAGCAAAAGCCTTTAGGTCCATTGCAGTTACTTTGTGCAGTAGAAGTAGTGAACTTTTTGTTGATGGAACAAATGTGAAAGTGAAATCCGACATAGAAAAACTGGAGGGCATGGTATTGGTAAGCCAGGATGGAAGTGTTCAATCTTCACCCTTTTTCCAGATATCTCAGGTGCTTTTGGTAGCTGACATAAATAACCTTATGGAGCTTATGCATGTTGAAGTAGATGTTCAATTTGATCATTTAGATGTATGGATTTCGCACAGCATTCTCTACTTCTGGCATGGTGTACAATTCAGTGTTGCTGAAGGAGGGCCTTCTCAATTTTCTTTTGGTAGAATTGATGTTAAAGTCCAGCTTAGGAAGGTTTCTTTTCTGCTAAGCGATGGAAGGTGGAGTTGTAGTGGACCCCTGTTTCAGATTCTCTTGGGGAACGTCCTCTTGCATATTACTGTGACTGAAAATAACCTCGAAGGTTTAGTTTCGGGTGACCTTCAAGTGAACTACAATAACATTCACAAGGTGTTCTGGGAGCCTTTTATTGAACCTTGGCAGTTTGAGGTCAATGTGATTAGAAAACAAGAGATGAGTCTCAGCAGCTCCAATTTGACAGATATCCATCTGAAATCAGCTGCACACCTTAATGTGAACTTCACTGAATCCCTTATAGAGTGTGTTTTCAGGACGGTTGAGATGATCAAGGATGCTTGGGTTCTAGTGAGTCCAAATGATCTTCCTGAAAGCCAAAAGTTATTAAATTCTCCGTTTGCTAAGCACACATATGCAGGAAAATATGCTCCTTATGTACTTCAAAACTTGACATCGCTGCCTCTAGCATATTGTGTTTATCAAGGCCCAATTAATTCTGATGAGTTGGATGTCTCAGAAACGAATAGAAAATATGTGCAACCAGGTTCTTCTATTCCAATATATATCGATGATACTCCTGAGAAACAACTTATTCATGTTAAGCCAGCACACTTCTCTGAGAGACTTTTTGACCAGAAGGCAAATGGGGTTCGCCATCAGTATATTACCATTCAACTTGATGGAACATCAGTGCCTTCTGAACCGATTTCAATGGATCTTGTAGGACTTACCTACTTTGAGGTTGATTTTTCTATGTCATACAGTGACAATATGGAAAACCATGGAACAAATGCCAATGCTGGTTTTGTTGTTCCTGTTGTCTTTGATGTTTCAGTGCAGCGGTACAGCAAATTAATCCGATTATACTCAACGGTTATACTTTCGAATTCAACTTCCATGCCATTGGAGCTGCGATTTGATATTCCATTTGGTGTGTCCCCAAAGATCTTAGATCCAATATATCCTGGTCAAGAGTTACCACTGCCTCTACACTTGGCCGAAGCTGGTAGGATTAGATGGCGTCCAATTGGCGATTCTCATCTCTGGAGTGAAGTTTATAACCTCTCAAACCTGCTATTTCAGGAGGGCAAAATTGGATTTCTCAAGTCTTTTGCTTGCTATCCAGCCCATCCCAATAGTGATCCATTTCGCTGTTGTATCTCAGTTAGAAACGTCAGTTTACCTTCACCTGTAAGGTCAAGAAAGAGTTCTTTAAAGCAAACAGTTGCAAGTGGTGGTCAAATATTGCACAAGGACGAGGCAAAAAAGCAGTTTATTCACCAAGTAGTCCTCAGTATCCCTTTAGTAGTGAATAACTATCTTCCCGATGCAGTGACATTGACGATAGAAAGCGGTGGGATTACCCGGACTGCATTTCTTTCAGAGGTGGAAACTTCTTTTCATAATGTTGATCCTTCTCATCAGCTGAGATTGGAGATCCATATGCATGGCTTTAAAACAGCTATTTTGGATTTTCCACGTACTGAAATATTCTGTAAAAAGGCTAAGTTTGGTGGCACGAAGTTCTCACTTTCTGAAATTGTGACCTTTGACAGAGACTCAACAAATGGTCCAATGTATGTCACAGTGGAAAAGGTGATGGATGCATTTTCTGGAGCCAGGGAACTCTTTATCTCTGTTCCATTTCTGTTATATAATTGCACTGGCTTTCCGCTCTTCATTTCTGAATCTGCCAGTGAAATGAAAGGAGTAAGCTGCATTGTGCCTTCTTGTTATGATATGGCTGATCAAGAAGTACTTCAGGGAAAAAAGGATGGTCTTGGTCTGGTATCTTCTAATCATAATCCAAATGCTAGAGATTCATATACAATGGGAAGTTCTTCCTCAAGAGTTCACATTGTCTCAATTAGAGACAATGCTACAACTCACAAAGAAATAAGTTTTAGCAAGCCTCTTTACTCTGAAGACAACTATCATGAGCTTTTAAGCAAACGTAATTTAGACAGGGAAAACTCACTTTCCAACAGTTCACAGAATAGATCGAGTTCAAGCAGTCAGTTGACCTCAAAAGATTTAAACTCTAGTGGTTATGAGCGTGGAAGAGTAAGGGCATGCATGTTCTCTCCTAAACCAATTTCCTCTGCTGGTGAAGTAATGGTTAGAGTGAGTAGGTGTATGCCTGAATATGTAACGGAAAAGATGCCAAATTCTCTATGGTCAAGTTCATTTCCCCTTATTCCACCTAGTGGTTCAACTACTGTCCTTGTTCCTCAACCATCGTCAAATGCGGCTTTTATGATGTCCATTACGTCCAGTGCAGTTGCTGCACCATTTGCTGGGAGGACAAGTGCAATTACCTTCCAACCTAGATACATCATCAGCAATGCATGCAGTAAGAACTTATGTTATAAGCAGAAAGGAACCGAATTTTCTTTTCAATTGGGTACAGGTGAACATTCTCATCTTCACTGGATGGATACAACCAGGGACTTACTCGTTTCAATTCGTTATAATGAGCCTGGATGGCAATGGTCAGGTGGCTTCTTGCCAGACCATCTCGGTGACACTCAAGTGAAAATGCGGAATTATCTCTCTGGTTCCTTAAATATGATACGGGTTGAAGTGCAAAATGCTGACGTCTCCGTGGGAGATGAAAAGATTGTTGGAAACTTCTATGGAAATTCTGGGACAAACTTGATTCTTGTGTCAGATGATGAGACTGGGTATATGCCCTACAGGGTTGATAATTTTACTAACGAGAGGCTAAGGATCTACCAACAAAGATGTGAAACCTTTGAGACTATTGTTCAGTCTTACACTTCTTGCCCTTATGCCTGGGATGAACCATGCTATCCACATCGTCTCACTGTGGAGGTACCTGGTAAGCGTGTTTTAGGGTCATACGCTCTTGATGATGTAAAAGAATACAGTCCGGTGCAATTACCATCATCTGCTGAGAAACCTGAGAGAACATTGCATATATCCATCCATGTTGAGGGAGCAACAAAGGTCCTCCATGTTATTGATTCAAGTTACCATGTCATGAATGACAACAGAAGTATTAGTCTACCTCACTTAAAAGACAAAGGGAAGCATGAACAGAAACAGGACAAGTTTATTGGTTTCATGGAAAGAATTTCAGTTTTTATTCAACAGATTGGCATCTCTGTGATCAATATTCACCCTCAGGAGTTGCTTTTTATATGTGCAAAGAGCATTACAGTTGATCTGGTACAGAGTTTGGATCAACAAAAACTTTCCTTCCAAATCGCATCATTGCAAGTCGATAATCAGCTTCGATCTTCCCCATATCCTGTTATGTTGTCTTTTGATCGTGAGTACAAAAACAATCCAGCTGGCCATGTGATTAGGGAGGATGATGTTAAACCAAGTGAAAGGATTTTGCAAAGGACTTCTCGCAATTTTGAACCTGTATTCTGCCTAGCTGTATCTAAGTGGAGGAAGAAAGATGTCTCACTGGTTTCGTTTGAATACATAAGCTTAAGAGTTGCAGATGTTTGTCTTGAGCTTGAACAAGAATTGATATTAAGCCTATTTGGCTTCATAAGAAATGTATCTTCAAGGTTCCAGAGTAGAGTCTTGCCATTATCAGATCCGTTCCTGCACCATCCTTCTAATGATGCTGGCTCAATGGATGCGTATGCAACTGATAACCAACTTCACTTAATGAGTGTTCCTCTGCTTAATGAAAACCACAAGCAAAGACTCTCACTACCTTCAATAGTTCCAATTGGAGCTCCTTGGCAGCAAATTTACCTTTTAGCTAGAAGACAGAAAAAGATATATGTAGAAGTGTTTGAATTTTCCCCCATCAAGTTGACCCTAAGCTTTTCCAGCACTCCATGGATGCTTAGAAATGGGATTCTTGCAGCAGGAGAATCTGTTATTCATAGAGGTCTGATGGCTCTTGCTGATGTGGAGGGTGCACGAATTCATCTGAAGCAGTTGACAATTGCACATCAAATTGCTAGCTTGGAGTCTCTACAAGAGATCCTTATGAGGCATTATACTCGGCAACTTCTTCATGAGATGTATAAGGTTTTTGGTTCAGCTGGTGTAATTGGCAATCCCATGGGATTTGCAAGGAGTTTAGGGCTTGGCATTAGAGACTTCTTGTCAGTTCCTGCCAGAAGCATTTTTCAGAGCCCTACTGGATTAATTACTGGCATGGCACAAGGCACGACTAGTCTCCTAAGTAATACTGTCTATGCTATAAGTGATGCTGCCACCCAATTCAGCAAAGCTGCACACAAGGGAATTGTGGCATTTACATTTGATGACCAGGCTGTTTCAGGGGTGCAACAGCAACAGACTGGTATAGCATCTCACTCTAAAGGTGTTATCAATGAAGTATTTGAGGGACTCACAGGTCTTCTTCAATCACCAATCAATGGAGCTGAGAAACATGGTCTTCCTGGTGTGCTCTCGGGCATAGCTTTAGGTATTACAGGACTTGTGGCCAAACCAGCTGCTAGTATTCTTGAAGTTACAGGAAAAACTGCACAGAGCATTAGAAACCGAAGTAGGATCTATCAGACAGGACAACAGCGCTTTAGAGTCCGTCTTCCAAGGCCTCTAAGCCGAGAATATCCCTTAAGACCTTACTGTTGGGAAGAAGCTGTTGGAGCATCAGTACTTGTTGAGGCAGATGATAGTTTGAGGCTCAAGGATGAAATTTTTGTTATGTGCAAGAAACTTAAAGAAGCTGGTAAATTTGTCATCATCACAGAGAGACTGGTTTTGATTGTAAGCTGCTCGAGTTTGGTGGACTTGGATAAGCCTGAATTTCGTGGTGTTCCTTCTGATCTCAAATGGGTGATTGAATCAGAAATTCATTTGGAGAGTGCGATGCATGCTGACTGCGATCAAGGAGTAGTTCATATAGTCGGCAGCAGCTCTAATACGCCTTTGCGTCAAAACCAGCTAGCCAAAAGAAGCAGTGGAACAAGGGCGGTGCGTTGGAATAATCCTACTGTTCCCCTCATTCAGACCAACTTGGAACTAGAACACAAAGAAGATGCGGAGAACTTGTTACAGGTTTTATTGTCTACAATTGAACTGGGAAAAGACCAAGGTTGGGGTTGTAGGAATATTCTGCACCGAAGTAATATCAAATAG

Protein Analysis

3238

Amino Acids

362.45

Weight (kDa)

5.77

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_N PF12624 36 - 528 1.2e-33 VPS13-like family N-terminal region
VPS13_M PF25033 1553 - 1649 4.4e-08 VPS13-like family middle region
VPS13_VAB PF25036 1816 - 2105 6.8e-09 Vacuolar-sorting associated protein 13, adaptor binding domain
VPS13_VAB PF25036 2215 - 2476 1.4e-23 Vacuolar-sorting associated protein 13, adaptor binding domain
VPS13_C PF25037 3053 - 3122 1.2e-08 Intermembrane lipid transfer protein VPS13, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 5676, 6987, 7089
AasI GACNNNNNNGTC 2 cut(s) 3095, 8967
AatII GACGTC 1 cut(s) 7218
AbsI CCTCGAGG 1 cut(s) 1115
Acc16I TGCGCA 2 cut(s) 387, 844
Acc36I ACCTGC 1 cut(s) 5697
Acc65I GGTACC 1 cut(s) 7453
AccB1I GGYRCC 1 cut(s) 7453
AccB7I CCANNNNNTGG 4 cut(s) 1975, 6824, 6914, 8990
AccBSI CCGCTC 1 cut(s) 6292
AccI GTMKAC 3 cut(s) 1279, 7645, 9647
AccII CGCG 1 cut(s) 2892
AcoI YGGCCR 5 cut(s) 898, 3036, 5613, 7936, 9057
AcuI CTGAAG 7 cut(s) 2787, 3209, 3632, 4575, 6362, 6561, 8558
AcyI GRCGYC 2 cut(s) 5638, 7215
AdeI CACNNNGTG 3 cut(s) 137, 4771, 6824
AfeI AGCGCT 1 cut(s) 9149
AflII CTTAAG 4 cut(s) 3530, 3841, 8080, 9193
AflIII ACRYGT 2 cut(s) 290, 2944
AhdI GACNNNNNGTC 1 cut(s) 8713
AhlI ACTAGT 2 cut(s) 2699, 8783
AjuI GAANNNNNNNTTGG 8 cut(s) 2976, 3008, 5635, 5667, 7224, 7256, 9204, 9236
AleI CACNNNNGTG 2 cut(s) 980, 7148
Alw21I GWGCWC 6 cut(s) 1792, 2363, 4130, 8338, 8527, 9024
Alw44I GTGCAC 1 cut(s) 8523
AlwNI CAGNNNCTG 2 cut(s) 6902, 8723
Ama87I CYCGRG 4 cut(s) 1115, 2549, 9025, 9355
Aor51HI AGCGCT 1 cut(s) 9149
ApaI GGGCCC 1 cut(s) 2909
ApaLI GTGCAC 1 cut(s) 8523
ArsI GACNNNNNNTTYG 2 cut(s) 4166, 4198
AseI ATTAAT 5 cut(s) 2810, 3705, 5109, 5471, 8756
Asp700I GAANNNNTTC 5 cut(s) 1493, 4139, 5824, 6375, 6457
Asp718I GGTACC 1 cut(s) 7453
AspA2I CCTAGG 2 cut(s) 2789, 3427
AspLEI GCGC 3 cut(s) 388, 845, 9150
AsuC2I CCSGG 1 cut(s) 5983
AsuII TTCGAA 1 cut(s) 5501
AsuNHI GCTAGC 2 cut(s) 8567, 9523
AvaI CYCGRG 4 cut(s) 1115, 2549, 9025, 9355
AvaII GGWCC 8 cut(s) 1240, 2445, 2793, 3298, 4266, 4655, 6188, 7588
AvrII CCTAGG 2 cut(s) 2789, 3427
AxyI CCTNAGG 2 cut(s) 3537, 7764
BaeGI GKGCMC 3 cut(s) 2909, 3186, 8527
BalI TGGCCA 4 cut(s) 900, 3038, 7938, 9059
BanI GGYRCC 1 cut(s) 7453
BanII GRGCYC 5 cut(s) 2363, 2909, 4130, 8338, 8747
BarI GAAGNNNNNNTAC 8 cut(s) 1710, 1742, 2029, 2061, 3966, 3998, 3999, 4031
BbsI GAAGAC 5 cut(s) 6549, 8374, 8963, 9002, 9155
Bbv12I GWGCWC 6 cut(s) 1792, 2363, 4130, 8338, 8527, 9024
BcgI CGANNNNNNTGC 4 cut(s) 2767, 2801, 8591, 8625
BciVI GTATCC 3 cut(s) 2391, 5924, 7048
BclI TGATCA 8 cut(s) 517, 2152, 3507, 3544, 4486, 4944, 6364, 7749
BcnI CCSGG 1 cut(s) 5983
BcuI ACTAGT 2 cut(s) 2699, 8783
BfmI CTRYAG 5 cut(s) 606, 963, 1084, 3597, 7312
BfoI RGCGCY 1 cut(s) 9151
BfrI CTTAAG 4 cut(s) 3530, 3841, 8080, 9193
BfuAI ACCTGC 1 cut(s) 5697
BfuI GTATCC 3 cut(s) 2391, 5924, 7048
BglI GCCNNNNNGGC 2 cut(s) 7102, 8136
BglII AGATCT 2 cut(s) 1546, 5562
BlnI CCTAGG 2 cut(s) 2789, 3427
BlpI GCTNAGC 3 cut(s) 3664, 4628, 5019
BmcAI AGTACT 4 cut(s) 200, 538, 6375, 9234
Bme18I GGWCC 8 cut(s) 1240, 2445, 2793, 3298, 4266, 4655, 6188, 7588
BmeRI GACNNNNNGTC 1 cut(s) 8713
BmeT110I CYCGRG 4 cut(s) 1115, 2549, 9025, 9355
BmrI ACTGGG 3 cut(s) 2451, 7308, 9668
BmtI GCTAGC 2 cut(s) 8571, 9527
BmuI ACTGGG 3 cut(s) 2451, 7308, 9668
BoxI GACNNNNGTC 2 cut(s) 717, 1285
BpiI GAAGAC 5 cut(s) 6549, 8374, 8963, 9002, 9155
BplI GAGNNNNNCTC 2 cut(s) 8260, 8292
BpmI CTGGAG 6 cut(s) 349, 720, 1919, 4373, 5683, 6252
Bpu10I CCTNAGC 3 cut(s) 4607, 7343, 8423
Bpu1102I GCTNAGC 3 cut(s) 3664, 4628, 5019
Bpu14I TTCGAA 1 cut(s) 5501
BpuEI CTTGAG 5 cut(s) 5705, 6448, 7131, 8123, 9248
BpuMI CCSGG 1 cut(s) 5983
Bsa29I ATCGAT 2 cut(s) 1748, 5184
BsaAI YACGTR 3 cut(s) 1530, 2945, 6098
BsaBI GATNNNNATC 4 cut(s) 2724, 4866, 5655, 7739
BsaHI GRCGYC 2 cut(s) 5638, 7215
BsaI GGTCTC 2 cut(s) 2412, 2920
BsaWI WCCGGW 1 cut(s) 7510
Bse21I CCTNAGG 2 cut(s) 3537, 7764
Bse3DI GCAATG 6 cut(s) 1767, 4269, 6967, 7112, 7550, 7853
Bse8I GATNNNNATC 4 cut(s) 2724, 4866, 5655, 7739
BseCI ATCGAT 2 cut(s) 1748, 5184
BseJI GATNNNNATC 4 cut(s) 2724, 4866, 5655, 7739
BseMI GCAATG 6 cut(s) 1767, 4269, 6967, 7112, 7550, 7853
BseSI GKGCMC 3 cut(s) 2909, 3186, 8527
BseYI CCCAGC 3 cut(s) 1765, 2638, 6914
BsgI GTGCAG 9 cut(s) 4306, 4866, 5474, 6888, 6915, 8186, 8829, 9084, 9680
Bsh1236I CGCG 1 cut(s) 2892
BshNI GGYRCC 1 cut(s) 7453
BshVI ATCGAT 2 cut(s) 1748, 5184
BsiHKAI GWGCWC 6 cut(s) 1792, 2363, 4130, 8338, 8527, 9024
BsiHKCI CYCGRG 4 cut(s) 1115, 2549, 9025, 9355
BsiSI CCGG 4 cut(s) 2290, 4088, 5983, 7511
BslFI GGGAC 9 cut(s) 309, 2532, 2591, 2788, 3284, 5539, 7079, 7279, 8973
BsmBI CGTCTC 2 cut(s) 7222, 7445
BsmFI GGGAC 9 cut(s) 309, 2532, 2591, 2788, 3284, 5539, 7079, 7279, 8973
BsmI GAATGC 3 cut(s) 2015, 2116, 4515
Bso31I GGTCTC 2 cut(s) 2412, 2920
BsoBI CYCGRG 4 cut(s) 1115, 2549, 9025, 9355
Bsp119I TTCGAA 1 cut(s) 5501
Bsp120I GGGCCC 1 cut(s) 2905
Bsp1286I GDGCHC 9 cut(s) 1792, 2363, 2909, 3186, 4130, 8338, 8527, 8747, 9024
Bsp1407I TGTACA 3 cut(s) 287, 3239, 4537
Bsp1720I GCTNAGC 3 cut(s) 3664, 4628, 5019
Bsp19I CCATGG 4 cut(s) 3102, 5398, 8441, 8670
BspDI ATCGAT 2 cut(s) 1748, 5184
BspFNI CGCG 1 cut(s) 2892
BspHI TCATGA 5 cut(s) 2752, 3504, 6553, 7620, 8623
BspMAI CTGCAG 2 cut(s) 610, 967
BspMI ACCTGC 1 cut(s) 5697
BspOI GCTAGC 2 cut(s) 8571, 9527
BspQI GCTCTTC 1 cut(s) 6299
BspT104I TTCGAA 1 cut(s) 5501
BspT107I GGYRCC 1 cut(s) 7453
BspTI CTTAAG 4 cut(s) 3530, 3841, 8080, 9193
BspTNI GGTCTC 2 cut(s) 2412, 2920
BsrBI CCGCTC 1 cut(s) 6292
BsrDI GCAATG 6 cut(s) 1767, 4269, 6967, 7112, 7550, 7853
BsrGI TGTACA 3 cut(s) 287, 3239, 4537
BssNI GRCGYC 2 cut(s) 5638, 7215
Bst6I CTCTTC 6 cut(s) 410, 646, 1425, 2806, 6299, 6673
BstACI GRCGYC 2 cut(s) 5638, 7215
BstAFI CTTAAG 4 cut(s) 3530, 3841, 8080, 9193
BstAPI GCANNNNNTGC 3 cut(s) 6902, 6911, 6966
BstAUI TGTACA 3 cut(s) 287, 3239, 4537
BstBAI YACGTR 3 cut(s) 1530, 2945, 6098
BstBI TTCGAA 1 cut(s) 5501
BstDSI CCRYGG 7 cut(s) 658, 3102, 3186, 5398, 7221, 8441, 8670
BstEII GGTNACC 2 cut(s) 2516, 4736
BstFNI CGCG 1 cut(s) 2892
BstH2I RGCGCY 1 cut(s) 9151
BstHHI GCGC 3 cut(s) 388, 845, 9150
BstNSI RCATGY 8 cut(s) 294, 2981, 3008, 4466, 6693, 6697, 6969, 9455
BstPAI GACNNNNGTC 2 cut(s) 717, 1285
BstPI GGTNACC 2 cut(s) 2516, 4736
BstSFI CTRYAG 5 cut(s) 606, 963, 1084, 3597, 7312
BstSLI GKGCMC 3 cut(s) 2909, 3186, 8527
BstUI CGCG 1 cut(s) 2892
BstV2I GAAGAC 5 cut(s) 6549, 8374, 8963, 9002, 9155
BstXI CCANNNNNNTGG 2 cut(s) 5521, 6065
Bsu15I ATCGAT 2 cut(s) 1748, 5184
Bsu36I CCTNAGG 2 cut(s) 3537, 7764
BsuI GTATCC 3 cut(s) 2391, 5924, 7048
BsuTUI ATCGAT 2 cut(s) 1748, 5184
BtgI CCRYGG 7 cut(s) 658, 3102, 3186, 5398, 7221, 8441, 8670
BtgZI GCGATG 3 cut(s) 4647, 5055, 9461
BtsI GCAGTG 5 cut(s) 878, 2269, 5597, 5955, 9544
BveI ACCTGC 1 cut(s) 5697
CaiI CAGNNNCTG 2 cut(s) 6902, 8723
CciI TCATGA 5 cut(s) 2752, 3504, 6553, 7620, 8623
CfoI GCGC 3 cut(s) 388, 845, 9150
ClaI ATCGAT 2 cut(s) 1748, 5184
CseI GACGC 3 cut(s) 1522, 5627, 9500
CsiI ACCWGGT 2 cut(s) 5158, 7456
CspCI CAANNNNNGTGG 4 cut(s) 1380, 1415, 2713, 2748
DraI TTTAAA 6 cut(s) 1212, 2937, 3336, 5832, 6073, 6654
DraIII CACNNNGTG 3 cut(s) 137, 4771, 6824
DrdI GACNNNNNNGTC 2 cut(s) 3095, 8967
DriI GACNNNNNGTC 1 cut(s) 8713
DseDI GACNNNNNNGTC 2 cut(s) 3095, 8967
EaeI YGGCCR 5 cut(s) 898, 3036, 5613, 7936, 9057
Eam1104I CTCTTC 6 cut(s) 410, 646, 1425, 2806, 6299, 6673
Eam1105I GACNNNNNGTC 1 cut(s) 8713
EarI CTCTTC 6 cut(s) 410, 646, 1425, 2806, 6299, 6673
Ecl136II GAGCTC 3 cut(s) 2361, 4128, 8336
Eco147I AGGCCT 2 cut(s) 1042, 9172
Eco24I GRGCYC 5 cut(s) 2363, 2909, 4130, 8338, 8747
Eco31I GGTCTC 2 cut(s) 2412, 2920
Eco32I GATATC 3 cut(s) 3580, 4413, 4864
Eco47I GGWCC 8 cut(s) 1240, 2445, 2793, 3298, 4266, 4655, 6188, 7588
Eco47III AGCGCT 1 cut(s) 9149
Eco53kI GAGCTC 3 cut(s) 2361, 4128, 8336
Eco57I CTGAAG 7 cut(s) 2787, 3209, 3632, 4575, 6362, 6561, 8558
Eco81I CCTNAGG 2 cut(s) 3537, 7764
Eco88I CYCGRG 4 cut(s) 1115, 2549, 9025, 9355
Eco91I GGTNACC 2 cut(s) 2516, 4736
EcoICRI GAGCTC 3 cut(s) 2361, 4128, 8336
EcoO109I RGGNCCY 7 cut(s) 1240, 2445, 2905, 2906, 3298, 4562, 7588
EcoO65I GGTNACC 2 cut(s) 2516, 4736
EcoRI GAATTC 2 cut(s) 5503, 8529
EcoRV GATATC 3 cut(s) 3580, 4413, 4864
EcoT22I ATGCAT 7 cut(s) 2768, 4464, 6066, 6226, 6695, 6967, 9453
EcoT38I GRGCYC 5 cut(s) 2363, 2909, 4130, 8338, 8747
Esp3I CGTCTC 2 cut(s) 7222, 7445
FaqI GGGAC 9 cut(s) 309, 2532, 2591, 2788, 3284, 5539, 7079, 7279, 8973
FauI CCCGC 1 cut(s) 201
FauNDI CATATG 3 cut(s) 2281, 5029, 6060
FbaI TGATCA 8 cut(s) 517, 2152, 3507, 3544, 4486, 4944, 6364, 7749
FblI GTMKAC 3 cut(s) 1279, 7645, 9647
FriOI GRGCYC 5 cut(s) 2363, 2909, 4130, 8338, 8747
FspAI RTGCGCAY 1 cut(s) 844
FspI TGCGCA 2 cut(s) 387, 844
GlaI GCGC 3 cut(s) 387, 844, 9149
GsaI CCCAGC 3 cut(s) 1769, 2642, 6918
GsuI CTGGAG 6 cut(s) 349, 720, 1919, 4373, 5683, 6252
HaeII RGCGCY 1 cut(s) 9151
HapII CCGG 4 cut(s) 2290, 4088, 5983, 7511
HgaI GACGC 3 cut(s) 1522, 5627, 9500
HhaI GCGC 3 cut(s) 388, 845, 9150
Hin1I GRCGYC 2 cut(s) 5638, 7215
Hin6I GCGC 3 cut(s) 386, 843, 9148
HinP1I GCGC 3 cut(s) 386, 843, 9148
HincII GTYRAC 7 cut(s) 163, 481, 1280, 4045, 6639, 8418, 8547
HindII GTYRAC 7 cut(s) 163, 481, 1280, 4045, 6639, 8418, 8547
HindIII AAGCTT 4 cut(s) 2184, 2667, 8077, 8425
HpaI GTTAAC 1 cut(s) 481
HpaII CCGG 4 cut(s) 2290, 4088, 5983, 7511
Hsp92I GRCGYC 2 cut(s) 5638, 7215
HspAI GCGC 3 cut(s) 386, 843, 9148
KflI GGGWCCC 1 cut(s) 3298
KpnI GGTACC 1 cut(s) 7457
Ksp22I TGATCA 8 cut(s) 517, 2152, 3507, 3544, 4486, 4944, 6364, 7749
KspAI GTTAAC 1 cut(s) 481
LguI GCTCTTC 1 cut(s) 6299
MabI ACCWGGT 2 cut(s) 5158, 7456
MbiI CCGCTC 1 cut(s) 6292
MfeI CAATTG 8 cut(s) 525, 1796, 1973, 5643, 7016, 8328, 8550, 9651
MhlI GDGCHC 9 cut(s) 1792, 2363, 2909, 3186, 4130, 8338, 8527, 8747, 9024
MlsI TGGCCA 4 cut(s) 900, 3038, 7938, 9059
MluNI TGGCCA 4 cut(s) 900, 3038, 7938, 9059
Mox20I TGGCCA 4 cut(s) 900, 3038, 7938, 9059
Mph1103I ATGCAT 7 cut(s) 2768, 4464, 6066, 6226, 6695, 6967, 9453
MroXI GAANNNNTTC 5 cut(s) 1493, 4139, 5824, 6375, 6457
MscI TGGCCA 4 cut(s) 900, 3038, 7938, 9059
Msp20I TGGCCA 4 cut(s) 900, 3038, 7938, 9059
MspA1I CMGCKG 8 cut(s) 212, 1515, 4880, 5458, 6043, 7934, 8651, 9068
MspCI CTTAAG 4 cut(s) 3530, 3841, 8080, 9193
MspI CCGG 4 cut(s) 2290, 4088, 5983, 7511
MunI CAATTG 8 cut(s) 525, 1796, 1973, 5643, 7016, 8328, 8550, 9651
Mva1269I GAATGC 3 cut(s) 2015, 2116, 4515
MvnI CGCG 1 cut(s) 2892
NciI CCSGG 1 cut(s) 5983
NcoI CCATGG 4 cut(s) 3102, 5398, 8441, 8670
NdeI CATATG 3 cut(s) 2281, 5029, 6060
NheI GCTAGC 2 cut(s) 8567, 9523
NmeAIII GCCGAG 2 cut(s) 8590, 9206
NsbI TGCGCA 2 cut(s) 387, 844
NsiI ATGCAT 7 cut(s) 2768, 4464, 6066, 6226, 6695, 6967, 9453
NspI RCATGY 8 cut(s) 294, 2981, 3008, 4466, 6693, 6697, 6969, 9455
NspV TTCGAA 1 cut(s) 5501
OliI CACNNNNGTG 2 cut(s) 980, 7148
PaeI GCATGC 3 cut(s) 6693, 6969, 9455
PaeR7I CTCGAG 2 cut(s) 1115, 9355
PagI TCATGA 5 cut(s) 2752, 3504, 6553, 7620, 8623
PceI AGGCCT 2 cut(s) 1042, 9172
PciI ACATGT 1 cut(s) 290
PciSI GCTCTTC 1 cut(s) 6299
PctI GAATGC 3 cut(s) 2015, 2116, 4515
PdmI GAANNNNTTC 5 cut(s) 1493, 4139, 5824, 6375, 6457
PflMI CCANNNNNTGG 4 cut(s) 1975, 6824, 6914, 8990
Ppu21I YACGTR 3 cut(s) 1530, 2945, 6098
PpuMI RGGWCCY 4 cut(s) 1240, 2445, 3298, 7588
PscI ACATGT 1 cut(s) 290
PshAI GACNNNNGTC 2 cut(s) 717, 1285
PshBI ATTAAT 5 cut(s) 2810, 3705, 5109, 5471, 8756
PsiI TTATAA 3 cut(s) 5676, 6987, 7089
Psp124BI GAGCTC 3 cut(s) 2363, 4130, 8338
Psp5II RGGWCCY 4 cut(s) 1240, 2445, 3298, 7588
PspEI GGTNACC 2 cut(s) 2516, 4736
PspFI CCCAGC 3 cut(s) 1765, 2638, 6914
PspOMI GGGCCC 1 cut(s) 2905
PspPPI RGGWCCY 4 cut(s) 1240, 2445, 3298, 7588
PspXI VCTCGAGB 2 cut(s) 1115, 9355
PstI CTGCAG 2 cut(s) 610, 967
PstNI CAGNNNCTG 2 cut(s) 6902, 8723
PvuII CAGCTG 6 cut(s) 1515, 4880, 6043, 7934, 8651, 9068
SacI GAGCTC 3 cut(s) 2363, 4130, 8338
SalI GTCGAC 1 cut(s) 1278
SapI GCTCTTC 1 cut(s) 6299
ScaI AGTACT 4 cut(s) 200, 538, 6375, 9234
SduI GDGCHC 9 cut(s) 1792, 2363, 2909, 3186, 4130, 8338, 8527, 8747, 9024
SexAI ACCWGGT 2 cut(s) 5158, 7456
SfcI CTRYAG 5 cut(s) 606, 963, 1084, 3597, 7312
Sfr274I CTCGAG 2 cut(s) 1115, 9355
SfuI TTCGAA 1 cut(s) 5501
SinI GGWCC 8 cut(s) 1240, 2445, 2793, 3298, 4266, 4655, 6188, 7588
SlaI CTCGAG 2 cut(s) 1115, 9355
SpeI ACTAGT 2 cut(s) 2699, 8783
SphI GCATGC 3 cut(s) 6693, 6969, 9455
SseBI AGGCCT 2 cut(s) 1042, 9172
SspI AATATT 6 cut(s) 1522, 1689, 5862, 6108, 7756, 9691
SstI GAGCTC 3 cut(s) 2363, 4130, 8338
StuI AGGCCT 2 cut(s) 1042, 9172
TaqII GACCGA 3 cut(s) 148, 2902, 4191
TatI WGTACW 9 cut(s) 198, 287, 536, 3239, 4537, 5053, 6373, 7917, 9232
TauI GCSGC 1 cut(s) 6869
Van91I CCANNNNNTGG 4 cut(s) 1975, 6824, 6914, 8990
Vha464I CTTAAG 4 cut(s) 3530, 3841, 8080, 9193
VneI GTGCAC 1 cut(s) 8523
VpaK11BI GGWCC 8 cut(s) 1240, 2445, 2793, 3298, 4266, 4655, 6188, 7588
VspI ATTAAT 5 cut(s) 2810, 3705, 5109, 5471, 8756
XceI RCATGY 8 cut(s) 294, 2981, 3008, 4466, 6693, 6697, 6969, 9455
XcmI CCANNNNNNNNNTGG 3 cut(s) 1894, 5258, 6789
XhoI CTCGAG 2 cut(s) 1115, 9355
XmaJI CCTAGG 2 cut(s) 2789, 3427
XmiI GTMKAC 3 cut(s) 1279, 7645, 9647
XmnI GAANNNNTTC 5 cut(s) 1493, 4139, 5824, 6375, 6457
ZraI GACGTC 1 cut(s) 7216
ZrmI AGTACT 4 cut(s) 200, 538, 6375, 9234
Zsp2I ATGCAT 7 cut(s) 2768, 4464, 6066, 6226, 6695, 6967, 9453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.