Rh2DG486000

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001657
Physical Location & Seq
Forward (+)
70142118 .. 70170279
28162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG486000.1

Sequence Viewer

Length: 10317 bp
ATGTTCGAAGGACTCGTCAGCCAGATTCTTCACGGCTATCTCGGTCGCTATGTCAAGGATTTTCACAAGCACCAGCTCAAGATGGAGATTTGGAATGAGGAAGTGTTCTTGGAAAACGTCGAGCTTAGTCTCGAAGCCTTTGATTATCTCCAGTTGCCTTTTGCTCTCAAACAAGGTCGGATTGGAAAGATAAGCATCAAAGTTCCATGGAAAACGCTTGGTGGGGACCCCTTTGTAATTAGTTTGGAAAATGTCTTCCTATGTGCATCCCAACGAGATGATGAAGAGTGGAGTTTGGATGAAGTTGAAAAACGAGAGTTTGCTGGAAAAAAGGCCAAACTTGCTGCAGCAGAACTGGCCAAATTATCAAAATGTGTATGCGAAAACCAGGCTGGGCTTAGATCAATAATTACTGCTAAGGTTCTTGACAGCATCCAAGTGTCAATCAGAGATTTTCATATCTTGTACCATGACAAGCTCAGTGGCTCGGCATGTAATATTTTTGGTTTGAAGATTTCCAGCTTGAGGACTATGAAGCAAAATCCTTCCGGGTCACTTGCAAAGGGAAGAGGGGGCCAAGTTAACAAAACTGTGGAGATTATGGGTTTGGAGTTTTACTGTGGCACACTTGATGGGGCGGTGGAGTTGATGGCTATGAACAATGATGGGGACTCTGTAGTACGGCAAGATATAAGATATGACGGGAAAAGATATAACAACTCCATACTGTCACCATGTGATGTTTCCATGTCACTCTCGGTCAACAGATCAGGAGAGCTTGATAGCAAAATCCCGCAGTACTCCATCACCGCTGAGATAACTGACTTGGTGATGTGCTTAGATGAGGTTCAGTTGCAACAGATTTTGTTTCTGTGGGATTATTTTTGTACATGTGAATTGAGAAAGAAGTATGGGCGTTATCGTCCCTGGAGTAGTCCGTTATCAAAAAAGGTGAAGGGTTGGCAAATGTTATGGTGGCATTATGCGCAAGAATCTATCTTATCAGATGTACGGAAGAGATTAAAGAAAACATCGTGGAGACACTTCGGACAGAGACTAAGCTCTTGTCGGAAGTATGTTAACCTATATAAGACTAAGCTGGATTTTCTTCGCCATGATCAGCCAATTGATGAAAGTACTCGATGGGAATTGGAGCAGATGGAGAAAGAATTAGACATAGATGATATTTTAAGTTACAGGTCGGCTGCAGAATGTGAGTTGCAGGAGTTTTTGGTGAACTCTTCAACTGGAAAATCCCGTGGATGGCTGAACTGGCTATCTCTTGGTATGCTTGGTGCTGGAGGAACAGATGACTTGGGTCAATTTTCTGGGGCTGTTTTTGTTTCTGATGCAGTTATTAAGGATATATATGAGGCGACAAAGTTCAATCCTCCTATTTTATCCAATGGGGATGCTCGAACAACTGATAAGATTGATATATGCGCACTCCATTTCAGCATCAATCGGATTTCTGCAACACTGCGTAACATGAAGTATGGCCAAGAAATTGCAGAGTTGATGTTGAATGGAGTGACTACGGAGTGCAAATTTAGGGAAGAATCTGCAGCCATTGTCACCATTGTGAACACAGGGCAGATGGTTTATCCTTGTAACAAGAAAGTCATTCTGCATTTGCGAAGGCCTAATAATGAGAAGAACGAAGTGGAAAACGAGAATCCTTCCTGTAGATTGCAAGTGGATGTATCATCAAACCTCGAGGCTGAATTGTCCATTAAGGGGATGCTTCAACCATTAGAAGTCACCATTGATGCAGAGTTTTTCTTAAAGTTGATGGATTTTCTTGGAGCTTTAAAATCCTTTGAAAGTCAGCATGGAAGGGTCCTTCTGTCGCTAAATGGGATAGAAAATGTCAATGGTCGACTGTTGTCGAAAGCTGAATACCTTTTATCACATCATAGGAAAGTGGTGTGGGATGTTACTATCTTCAATATCATCATAAATGTTCCTTGGAGAGAGGCAATATCTGATCCACATAACTTGGTTTTTGAAGCAGGCTCTCTTCTTTTTAGGACTAAATGTGATATGGGATCTCGGCCATCAGATGATGAGGAACAATTTTATACTCTGAAAAAGTTCTTAACTTCAGTTTCCAGCTGTAATATTTCTCCATGCGTCCAAATTCAAGATCTGTACGATCACTATGAGGTCAAGTTAAATGATTTTGAGTTGAAGGTAACGATGCCTTCTCATTCTTCTCCAATTTCTATCTTGGAGAAACTTAGTCCTTCTGTCTCCTTTGCATTTTGCTTGATCCAAGATGAGTCAATATTGAAACAGTTGGAGGCTTGTGTCATTGTATCATCACTTCATGCACATTTTTCTCCATCGATATATGCTGCAATGCTGGGATTGATAGAATATCTAGGTGCTCTGCAAGTGAATTTTGATTCTCTACCCTTGGGAACTATTGATTCACTTGATGCCACATCAAATGGATTAGGGAATCCTGTTTTCGGTTTCTCTACCAATATCAAACTGGAGACAGTGAAGATTGAAGTCGAACTGGAAAATGAAGAAGAAAATAGCTCGTCATTCATGGTTAAATTCCAACAATTGGACATGGGTTATTCGCTTTCAAAAATTGAGGAATGCTGGATCTTAATGAACGCATTGAGTATCACCACTTCTAAATTGGCTGATAAAAGTGATAGTCGGATTTTGTATTCATCTGGAAATCAATCATCTATGAATGCTTTACATCCACATGAGATTGGGATTGGCAATAGAAATGATCATTTTGCCAACACCATTGGAAATAATGAAGCATGCTTTACTTTACAATATGAATCTCATCCGAAGGAGACTGTTCCTAAAAAATGTAGAATATGCTTGAATAACGGTGATCTCCACTGCTACCCATATGTTATCCGGCTTCTGGTTGCATTTTTTGATAAACTTTCTACTTATGGTGCATCTAATCCTGGTAAGAACACTTTGAGCTCATCTGTGGATGCTAGGTATCCAAAAAGTGTTCCTGGTTTTGGGTCTCAAAGGTTTGGTTTCTCCAACTTTGTAGAAACTGGGTCCTCTGAGTATGCAAGCATACCTTTAGATCATTTTCCTTTTGTTATGCTATCCAATGCTGGTTCCCTTGGTAACCTTGAAAGTTCTTTGCTTTATGCCAGTCCCGAGTGGAGAAAGTATTTCAGTATAAGGGACAGCAGAATCAGCAGCCCACTATCCAGCATAAAGGAGGTATCTAAAAAGTTCCATGCCCCAGCATTGAAGCACTCATCTGTGGTTGAAGCTTCTGCTGCGTGTGGGAGTTCTGCCACTACTAGTAGTCCGCTTGTAATTGATGTCAATCTCTGTGGAATCAGAGTGCATTTTCATGATTCAAAATGCATTGTTGGGACAATTACCGTGCCTAGGTCCAACTCTTCAGTATTAATTTCTGAAAACAGCTTTGATATATTGTGTTCTACTGAGGGGTTAGTTCTCAGTTCATCTTGGTCATCACAGAATTTACGCGAGTTTCTGTGGGGCCCCTCGGTCTTGAATTTATCTCCTATTTTAAATGTACGTGTGAGGAAAGAATGTGGGCCATTGAGTTCGCATGTTGAACTATGCTTTGGCATACAGCATGTATATTGCATCTTACCTCCTGAATATCTGGCCATAATAATTGGTTACTTTTCGTCGTCTGATTGGAGTTCAGATTCTAATGACCAGCTTGTCACCATGGGGCATGAAGATACTGAAAGTGAAAGTGAATGTTCCTTTATCTACAAGATTGAGATACTGGACTCTGTTTTAGTTGTGCCCGTGGAAAGCAACGAGGGTCAGTTTCTAAAGAGCGAACTTCAGCAGTTCTACTGTACATTTATACAAAGCAGTTCAAATAATGTATTGAAGGACATTCCCCATGAATGTTGGGTCCCGACTGATAAACTTGCTAAAAGAAATCATTGTTTAAATTTATTTGGACGAGACTTGTTTCTATCATTCCTGTCTTTCAAGGACAATCAATACAATTCCTTTGTAGATGTTCCGTTAATTGCTTCCCTAGGTGCTGACGTTTGGGTGAAAATACCTTGTGAAAGTGAATCGTCATGTGAAAGTTCTCCCTCAAACATTTGTGTCATGATCAGAGTTGGAAACTGTCAGCTTAAGCCTGAGGATGATCACTTTTTTTATGGATTTGAGGGGCTAAATGATATCATAAATCAATTTTCTGTAGTTTCTGATGTATCTGAATGTTTCAAGTCTGATGTTTTACAATTTCTTCAGTCAAAAAGGTGCTTAGCACAGAAAAATGCAGTTCCACCTGTTGTTTCAAGCATTAATTATACTGAAGTTAGATGCTATATCAATTCATTGTTGATACAGTTGAATCCCTGCCAAAGAGTCTCGGAAGATCCTATTGCTACTGCTGAAATGAAATTCATCTGCTCTGCATCATTGAGAAATGACACTCTCTTAAGTGTAGCTCTAAGTTTTTCTTCCCTAGAAATATTTTCATTACCCAATTCTTTTGTATTAGCAAGATGCAAGTCCACAAGCTCAACCTCGTCAGTTCTTGAATTTTCTCTTACCAAAGAAAAAGATGGTGTAAATGAACTTCACGTTTCCTTGCCTTGTGTTGAAGTGTGGCTACATTTGTCTTACTGGAATGAGGTTGTTGACCTTTTCAAATCTTATGCTGGACAATCATCCACAAGTCTGCCGGACAACTTAGAGCAGGACACTGATGCCCTCATTGTGAGCTCAGAGAATATTTTCATTACATTTTACTTACCTGTTTGGACCGATGACCGAGCTTTTGGGGAATATCAGGGAGAGGAATGTCATGGAGATGGCTCTACAAATGGGTTATCTGACATAGTTGAAGCAAAAGCCTTTAGGTCCATTGCAGTTACTTTGTGCAGCAGAAGTAGTGAACTTTTTGTTGATGGAACAAATGTGAAAGTGAAATCCGACGTAGAAAAACTGGAGGGCATGGTATTGGTAAGCCAGGATGGAAGTGTTCAATCTTCACCCTTTTTCCAGATATCTCAGGTGCTTTTGGTAGCTGACATAAATAACCTTATGGAGCTTATGCATGTTGAAGTAGATGTTCAATTTGATCAATTAGATGTATGGATTTCGCACAGCATTCTCTACTTCTGGCATGGTGTACAATTCAGTGTTGCTGAAGGAGGGCCTTCTCAATTTTCTTTTGGTAGAATTGATGTTAAAGTCCAGCTTAGGAAGGTTTCTTTTCTGCTAAGCGATGGAAGGTGGAGTTGTAGTGGACCCCTGTTTCAGATTCTCTTGGGGAACATCCTCTTGCATATTACTGTGACTGAAAATAACCTCGAAGGTGTAGTTTCGGGTGACCTTCAAGTAAACTACAATAACATTCACAAGGTGTTCTGGGAGCCTTTTATTGAACCTTGGCAGTTTGAGGTCAATGTGATTAGAAAACAAGAGATGAGTCTCAGCAGCTCCAATTTCACAGATATCCATCTGAAATCAGCTGCACACCTTAATGTGAACTTCACTGAATCCCTTATAGAGTGTGTTTTCAGGACAGTTGAGATGATCAAGGATGCTTGGGTTCTAGTGAGTCCAAATGATCTTCCTGAAAGCCAAAAGTTATTAAATTCTCCGTTTGCTAAGCACACATATGCAGGAAAATATGCTCCTTATGTACTTCAAAACTTGACATCGCTGCCTCTAGCATATCGTGTTTATCAAGGCCCAATTAATTCTGACGAGTTGGATGTCTCAGAAACGAATAGAAAATATGTGCAACCAGGTTCTTCCATTCCAATATATATCGATGATACTCCTGAGAAACAACTTATTCATGTTAAGCCAGCACACTTCTCTGAGAGACTTTTTGACCAGAAGGCAAATGGGGTTCGCCATCAGTATATTACCATTCAACTTGATGGAACATCAGTGCCTTCTGAACCGATTTCAATGGATCTTGTAGGACTTACCTACTTTGAGGTTGATTTTTCTATGCCATACAGTGACAATATGGAAAACCATGGAACAAATGCCAATGCTGGTTTTGTTGTTCCTGTTGTCTTTGATGTTTCAGTGCAGCGGTACAGCAAATTAATCCGATTATACTCAACGGTTATACTTTCGAATTCAACTTCCATGCCATTGGAGCTGCGATTTGATATTCCATTTGGTGTGTCCCCAAAGATCTTAGATCCAATATATCCTGGTCAAGAGTTACCACTGCCTCTACACTTGGCCGAAGCTGGTAGGATTAGATGGCGTCCAATTGGCGATTCTCATCTCTGGAGTGAAGTTTATAACCTCTCAAACCTGCTTTTTCAGGAGGGCAAAATTGGATTTCTCAAGTCTTTTGCTTGCTATCCAGCCCATCCCAATAGTGATCCATTTCGCTGTTGTATCTCAGTTAGAAACGTCAGTTTACCTTCACCTGTAAGGTCAAGAAAGAGTTCTTTAAAGCAAACAGTTGCAAGTGGTGGTCAAATATTGCACAAGGACGAGGCAAAAAAGCAGTTTATTCACCAAGTAGTCCTCAGTATCCCTTTAGTAGTGAATAACTATCTTCCCGATGCAGTGACATTGACGATAGAAAGCGGTGGGATTACCCGGACTGCATTTCTTTCAGAGGTGGAAACTTCTTTTCATAATGTTGACCCTTCTCATCAGCTGAGATTGGAGATCCATATGCATGGCTTTAAAACAGCTATTTTGGATTTTCCACGTACTGAAATATTCTGTAAAAAGGCTAAGTTTGGTGGCACGAAGTTCTCACTTTCTGAAATTGTGACCTTTGACAGAGACTCAACAAATGGTCCAATGTATGTCACAGTGGAAAAGGTGATGGATGCATTTTCTGGAGCCAGGGAACTCTTTATCTCTGTTCCATTTCTGTTATATAATTGCACTGGCTTTCCGCTCTTCATTTCTGAATCTGCCAGTGAAATGAAAGGAGTAAGCTGCATTGTGCCTTCTTGTTATGATATGGCTGATCAAGAAGTACTTCAGGGAAAAAAGGATGGTCTTGGTCTGGTATCTTCTAATCATAATCCAAATGCTAGAGATTCATATACAATGGGAAGTTCTTCCTCAAGAGTTCACATTGTCTCAATTAGAGACAATGCTACAACTCACAAAGAAATAAGTTTTAGCAAGCCTCTTTACTCTGAAGACAACTATCATGAGCTTTTAAGCAAACGTAATTTAGACAGGGAAAACTCACTTTCCAGCAGTTCACAGAATAGATCAAGTTCAAGCAGTCAGTTGACCTCAAAAGATTTAAACTCTAGTCGTTATGAGCGTGGAAGAGTAAGGGCATGCATGTTCTCTCCTAAACCAATTTCCTCTGCTGGTGAAGTAATGGTTAGAGTGAGTAGGTGTATGCCTGAATATGTAACGGAAAAGATGCCAAATTCTCTATGGTCAAGTTCATTTCCCCTTATTCCGCCTAGTGGTTCAACTACTGTCCTTGTTCCTCAACCATCGTCAAATGCGGCTTTTATGATGTCCATTACGTCCAGTGCAGTTGCCGCACCATTTGCTGGGAGGACAAGTGCAATTACCTTCCAACCTAGATACATCATCAGCAATGCATGCAGTAAGAACTTATGTTATAAGCAGAAAGGAACCGAATTTTCTTTTCAATTGGGTACAGGGGAACATTCTCATCTTCACTGGATGGATACAACCAGGGAGTTACTCGTTTCAATTCGTTATAATGAGCCTGGATGGCAATGGTCAGGTGGCTTCTTGCCAGACCATCTCGGTGACACTCAAGTGAAAATGCGGAATTATCTCTCTGGTTCCTTAAATATGATACGGGTTGAAGTGCAAAATGCTGACGTCTCCGTGGGAGATGAAAAGATTGTTGGAAACTTCTATGGAAATTCTGGGACAAACTTGATTCTTGTGTCAGATGATGAGACTGGGTATATGCCCTACAGGGTTGATAATTTTACTAATGAGAGGCTAAGGATCTACCAACAAAGATGTGAAACCTTTGAGACTATTGTTCAGTCTTACACTTCTTGCCCTTATGCCTGGGATGAACCATGCTATCCACATCGTCTCACTGTGGAGGTACCTGGTAAGCGTGTTTTAGGGTCATACGCTCTTGATGATGTAAAAGAATACAGTCCGGTGCAATTACCATCATCTGCTGAGAAACCTGAGAGAACATTGCATATATCCATCCATGTTGAGGGAGCAACAAAGGTCCTCCATGTTATTGATTCAAGTTACCATGTCATGAATGACAACAGAAGTATTAGTCTACCTCACTTAAAAGACAAAGGGAAGCATGAACAGAAACAGGACAAGTTTATTGGTTTCATGGAAAGAATTTCAGTTTTTATTCAACAGATTGGCATCTCTGTGATCAATATTCACCCTCAGGAGTTGCTTTTTATATGTGCAAAGAGCATTACAGTTGATCTGGTACAAAGTTTGGATCAACAAAAACTTTCCATCCAAATCGCATCATTGCAAGTCGATAATCAGCTTCGATCTTCCCCATATCCTGTTATGTTGTCTTTTGATCGTGAGTACAAAAACAATCCAGCTGGCCATGTGATTAGGGAGGATGATGTTAAACCAAGTGAAAGGATTTTGCAAAGGACTTCTCGCAGTTTTGAACCTGTATTCTGCCTAGCTGTATCTAAGTGGAGGAAGAAAGATGTCTCACTGGTTTCGTTTGAATACATAAGCTTAAGAGTTGCAGATGTTTGTCTTGAGCTTGAACAAGAATTGATATTAAGCCTATTTGGCTTCATAAGAAATGTATCTTCAAGGTTCCAGAGTAGAGTCTTGCCATTATCAGATCCGTTCCTGCACCATCCTTCTAATGATGCTGGCTCAATGGATGCGTATGCAACTGATAACCAACTTCACTTAATGAGTGTTCCTCTGCTTAATGAAAACCACAAGCAAAGACTCTCACTACCTTCAATAGTTCCAATTGGAGCTCCTTGGCAGCAAATTTACCTTTTAGCTAGAAGACAGAAAAAGATATATGTAGAAGTGTTTGAATTTTCCCCCATCAAGTTGACCCTAAGCTTTTCCAGCACTCCATGGATGCTTAGAAATGGGATTCTTGCAGCAGGAGAATCTGTTATTCATAGAGGTCTGATGGCTCTTGCTGATGTGGAGGGTGCACGAATTCATCTGAAGCAGTTGACAATTGCACATCAAATTGCTAGCTTGGAGTCTCTACAAGAGATCCTTATGAGGCATTATACTCGGCAACTTCTTCATGAGATGTATAAGGTTTTTGGTTCAGCTGGTGTAATTGGCAATCCCATGGGATTTGCAAGGAGTTTAGGGCTTGGCATTAGAGACTTCTTGTCAGTGCCTGCCAGAAGCATTTTTCAGAGCCCTACTGGATTAATTACTGGCATGGCACAAGGCACGACTAGTCTCCTAAGTAATACAGTCTATGCTATAAGTGATGCTGCCACCCAATTCAGCAAAGCTGCGCACAAGGGAATTGTGGCATTTACATTTGATGACCAGGCTGTTTCAGGGGTGCAACAGCAACAGACTGGTATAGCATCTCACTCTAAAGGTGTTATCAATGAAGTATTTGAGGGACTCACAGGTCTTCTTCAATCACCAATCAATGGAGCTGAGAAACATGGTCTTCCTGGTGTGCTCTCAGGCATAGCTTTAGGTATTACAGGACTTGTGGCCAAACCAGCTGCTAGTATTCTTGAAGTTACAGGAAAAACTGCACAGAGCATTAGAAACCGAAGTAGGATCTATCAGACAGGACAACAGCGCTTTAGAGTCCGTCTTCCAAGGCCTCTAAGCCGAGAATATCCCTTAAGACCTTACTGTTGGGAAGAAGCTGTTGGAGCAGCAGTACTTGTTGAGGCAGATGATAGTTTGAGGCTCAAGGATGAAATTTTTGTTATGTGCAAGAAACTTAAAGAAGCTGGTAAATTTGTCATCATCACAGAGAGACTGGTTTTGATTGTAAGCTGCTCAAGTTTGGTGGACTTGAGTAAGCCTGAATTTCGTGGTGTTCCTTCTGATCTCAAATGGGTGATTGAATCAGAAATTCATTTGGAGAGTGCGATGCATGCTGACTGCGATCAAGGAGTAGTTCATATAGTCGGCAGCAGCTCTAATACGCCTTTGCGTCAAAACCAGCTAGCCAAAAGAAGCGGTGGAACAAGGGCGGTGCGTTGGAATAATCCTACTGTTCCCCTCATTCAGACCAACTTGGAACTAGAACACAAAGAAGATGCGGAGAACTTGTTACAGGTTTTATTGTCTACAATTGAACTGGGAAAAGACCAAGGTTGGGGTTGTAGGAATATTCTGCACCGAAGTAATATCAAATAG

Protein Analysis

3438

Amino Acids

384.86

Weight (kDa)

5.91

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_N PF12624 1 - 728 6.8e-77 VPS13-like family N-terminal region
VPS13_M PF25033 1753 - 1848 1.3e-08 VPS13-like family middle region
VPS13_VAB PF25036 2016 - 2305 7.2e-09 Vacuolar-sorting associated protein 13, adaptor binding domain
VPS13_VAB PF25036 2412 - 2676 9.3e-24 Vacuolar-sorting associated protein 13, adaptor binding domain
VPS13_C PF25037 3253 - 3322 1.1e-08 Intermembrane lipid transfer protein VPS13, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 6276, 7587, 7689
AasI GACNNNNNNGTC 2 cut(s) 3695, 9567
AatII GACGTC 1 cut(s) 7818
AbsI CCTCGAGG 1 cut(s) 1715
Acc16I TGCGCA 3 cut(s) 987, 1444, 9447
Acc36I ACCTGC 1 cut(s) 6297
Acc65I GGTACC 1 cut(s) 8053
AccB1I GGYRCC 1 cut(s) 8053
AccB7I CCANNNNNTGG 3 cut(s) 2575, 7514, 9590
AccBSI CCGCTC 1 cut(s) 6892
AccI GTMKAC 3 cut(s) 1879, 8245, 10247
AccII CGCG 1 cut(s) 3492
AcoI YGGCCR 7 cut(s) 357, 1498, 2054, 3636, 6213, 8536, 9657
AcuI CTGAAG 9 cut(s) 2088, 3387, 3809, 4232, 4335, 5175, 6962, 7161, 9158
AcyI GRCGYC 2 cut(s) 6238, 7815
AdeI CACNNNGTG 2 cut(s) 737, 5371
AfeI AGCGCT 1 cut(s) 9749
AflII CTTAAG 4 cut(s) 4130, 4441, 8680, 9793
AflIII ACRYGT 2 cut(s) 890, 3544
AhdI GACNNNNNGTC 1 cut(s) 9313
AhlI ACTAGT 2 cut(s) 3299, 9383
AjuI GAANNNNNNNTTGG 8 cut(s) 3576, 3608, 6235, 6267, 7824, 7856, 9804, 9836
AleI CACNNNNGTG 2 cut(s) 1580, 7748
Alw21I GWGCWC 6 cut(s) 2392, 2963, 4730, 8938, 9127, 9624
Alw44I GTGCAC 1 cut(s) 9123
AlwNI CAGNNNCTG 1 cut(s) 9323
Ama87I CYCGRG 2 cut(s) 1715, 3149
Aor51HI AGCGCT 1 cut(s) 9749
ApaI GGGCCC 1 cut(s) 3509
ApaLI GTGCAC 1 cut(s) 9123
ArsI GACNNNNNNTTYG 3 cut(s) 32, 4766, 4798
AseI ATTAAT 5 cut(s) 3410, 4305, 5709, 6071, 9356
Asp700I GAANNNNTTC 5 cut(s) 2093, 4739, 6424, 6975, 7057
Asp718I GGTACC 1 cut(s) 8053
AspA2I CCTAGG 2 cut(s) 3389, 4027
AspLEI GCGC 4 cut(s) 988, 1445, 9448, 9750
AsuC2I CCSGG 2 cut(s) 550, 6583
AsuII TTCGAA 2 cut(s) 6, 6101
AsuNHI GCTAGC 2 cut(s) 9167, 10123
AvaI CYCGRG 2 cut(s) 1715, 3149
AvrII CCTAGG 2 cut(s) 3389, 4027
AxyI CCTNAGG 2 cut(s) 4137, 8364
BaeGI GKGCMC 3 cut(s) 3509, 3786, 9127
BalI TGGCCA 5 cut(s) 359, 1500, 3638, 8538, 9659
BanI GGYRCC 1 cut(s) 8053
BanII GRGCYC 5 cut(s) 2963, 3509, 4730, 8938, 9347
BarI GAAGNNNNNNTAC 8 cut(s) 2310, 2342, 2629, 2661, 4566, 4598, 4599, 4631
BbsI GAAGAC 6 cut(s) 247, 7149, 8974, 9563, 9602, 9755
Bbv12I GWGCWC 6 cut(s) 2392, 2963, 4730, 8938, 9127, 9624
BceAI ACGGC 2 cut(s) 49, 698
BcgI CGANNNNNNTGC 2 cut(s) 9191, 9225
BciVI GTATCC 3 cut(s) 2991, 6524, 7648
BclI TGATCA 8 cut(s) 1117, 2752, 4107, 4144, 5086, 5544, 6964, 8349
BcnI CCSGG 2 cut(s) 550, 6583
BcuI ACTAGT 2 cut(s) 3299, 9383
BfmI CTRYAG 7 cut(s) 345, 675, 1206, 1563, 1684, 4197, 7912
BfoI RGCGCY 1 cut(s) 9751
BfrI CTTAAG 4 cut(s) 4130, 4441, 8680, 9793
BfuAI ACCTGC 1 cut(s) 6297
BfuI GTATCC 3 cut(s) 2991, 6524, 7648
BglI GCCNNNNNGGC 2 cut(s) 7702, 8736
BglII AGATCT 2 cut(s) 2146, 6162
BlnI CCTAGG 2 cut(s) 3389, 4027
BlpI GCTNAGC 3 cut(s) 4264, 5228, 5619
BmcAI AGTACT 4 cut(s) 800, 1138, 6975, 9834
BmeRI GACNNNNNGTC 1 cut(s) 9313
BmeT110I CYCGRG 2 cut(s) 1715, 3149
BmrI ACTGGG 3 cut(s) 3051, 7908, 10268
BmtI GCTAGC 2 cut(s) 9171, 10127
BmuI ACTGGG 3 cut(s) 3051, 7908, 10268
BoxI GACNNNNGTC 2 cut(s) 1317, 1885
BpiI GAAGAC 6 cut(s) 247, 7149, 8974, 9563, 9602, 9755
BplI GAGNNNNNCTC 2 cut(s) 8860, 8892
BpmI CTGGAG 7 cut(s) 134, 949, 1320, 2519, 4973, 6283, 6852
Bpu10I CCTNAGC 4 cut(s) 417, 5207, 7943, 9023
Bpu1102I GCTNAGC 3 cut(s) 4264, 5228, 5619
Bpu14I TTCGAA 2 cut(s) 6, 6101
BpuEI CTTGAG 9 cut(s) 62, 544, 6305, 7048, 7731, 8723, 9848, 9940, 9991
BpuMI CCSGG 2 cut(s) 550, 6583
Bsa29I ATCGAT 2 cut(s) 2348, 5784
BsaAI YACGTR 2 cut(s) 3545, 6698
BsaBI GATNNNNATC 5 cut(s) 194, 3324, 5466, 6255, 8339
BsaHI GRCGYC 2 cut(s) 6238, 7815
BsaI GGTCTC 1 cut(s) 3012
BsaWI WCCGGW 1 cut(s) 8110
Bse21I CCTNAGG 2 cut(s) 4137, 8364
Bse3DI GCAATG 6 cut(s) 2367, 4869, 7567, 7712, 8150, 8453
Bse8I GATNNNNATC 5 cut(s) 194, 3324, 5466, 6255, 8339
BseCI ATCGAT 2 cut(s) 2348, 5784
BseJI GATNNNNATC 5 cut(s) 194, 3324, 5466, 6255, 8339
BseMI GCAATG 6 cut(s) 2367, 4869, 7567, 7712, 8150, 8453
BseSI GKGCMC 3 cut(s) 3509, 3786, 9127
BseYI CCCAGC 4 cut(s) 392, 2365, 3238, 7514
BsgI GTGCAG 7 cut(s) 4906, 5466, 6074, 7515, 8786, 9684, 10280
Bsh1236I CGCG 1 cut(s) 3492
Bsh1285I CGRYCG 1 cut(s) 46
BshNI GGYRCC 1 cut(s) 8053
BshVI ATCGAT 2 cut(s) 2348, 5784
BsiEI CGRYCG 1 cut(s) 46
BsiHKAI GWGCWC 6 cut(s) 2392, 2963, 4730, 8938, 9127, 9624
BsiHKCI CYCGRG 2 cut(s) 1715, 3149
BsiSI CCGG 5 cut(s) 549, 2890, 4688, 6583, 8111
BsmBI CGTCTC 2 cut(s) 7822, 8045
BsmI GAATGC 3 cut(s) 2615, 2716, 5115
Bso31I GGTCTC 1 cut(s) 3012
BsoBI CYCGRG 2 cut(s) 1715, 3149
Bsp119I TTCGAA 2 cut(s) 6, 6101
Bsp120I GGGCCC 1 cut(s) 3505
Bsp1286I GDGCHC 9 cut(s) 2392, 2963, 3509, 3786, 4730, 8938, 9127, 9347, 9624
Bsp1407I TGTACA 3 cut(s) 887, 3839, 5137
Bsp1720I GCTNAGC 3 cut(s) 4264, 5228, 5619
Bsp19I CCATGG 5 cut(s) 206, 3702, 5998, 9041, 9270
BspDI ATCGAT 2 cut(s) 2348, 5784
BspFNI CGCG 1 cut(s) 3492
BspHI TCATGA 5 cut(s) 3352, 4104, 7153, 8220, 9223
BspMAI CTGCAG 3 cut(s) 349, 1210, 1567
BspMI ACCTGC 1 cut(s) 6297
BspOI GCTAGC 2 cut(s) 9171, 10127
BspQI GCTCTTC 1 cut(s) 6899
BspT104I TTCGAA 2 cut(s) 6, 6101
BspT107I GGYRCC 1 cut(s) 8053
BspTI CTTAAG 4 cut(s) 4130, 4441, 8680, 9793
BspTNI GGTCTC 1 cut(s) 3012
BsrBI CCGCTC 1 cut(s) 6892
BsrDI GCAATG 6 cut(s) 2367, 4869, 7567, 7712, 8150, 8453
BsrGI TGTACA 3 cut(s) 887, 3839, 5137
BssNI GRCGYC 2 cut(s) 6238, 7815
Bst6I CTCTTC 8 cut(s) 279, 562, 1010, 1246, 2025, 3406, 6899, 7273
BstACI GRCGYC 2 cut(s) 6238, 7815
BstAFI CTTAAG 4 cut(s) 4130, 4441, 8680, 9793
BstAPI GCANNNNNTGC 2 cut(s) 7511, 7566
BstAUI TGTACA 3 cut(s) 887, 3839, 5137
BstBAI YACGTR 2 cut(s) 3545, 6698
BstBI TTCGAA 2 cut(s) 6, 6101
BstDSI CCRYGG 8 cut(s) 206, 1258, 3702, 3786, 5998, 7821, 9041, 9270
BstEII GGTNACC 2 cut(s) 3116, 5336
BstFNI CGCG 1 cut(s) 3492
BstH2I RGCGCY 1 cut(s) 9751
BstHHI GCGC 4 cut(s) 988, 1445, 9448, 9750
BstMCI CGRYCG 1 cut(s) 46
BstPAI GACNNNNGTC 2 cut(s) 1317, 1885
BstPI GGTNACC 2 cut(s) 3116, 5336
BstSFI CTRYAG 7 cut(s) 345, 675, 1206, 1563, 1684, 4197, 7912
BstSLI GKGCMC 3 cut(s) 3509, 3786, 9127
BstUI CGCG 1 cut(s) 3492
BstV2I GAAGAC 6 cut(s) 247, 7149, 8974, 9563, 9602, 9755
BstXI CCANNNNNNTGG 2 cut(s) 6121, 6665
Bsu15I ATCGAT 2 cut(s) 2348, 5784
Bsu36I CCTNAGG 2 cut(s) 4137, 8364
BsuI GTATCC 3 cut(s) 2991, 6524, 7648
BsuTUI ATCGAT 2 cut(s) 2348, 5784
BtgI CCRYGG 8 cut(s) 206, 1258, 3702, 3786, 5998, 7821, 9041, 9270
BtgZI GCGATG 3 cut(s) 5247, 5655, 10061
BtsI GCAGTG 4 cut(s) 1478, 2869, 6197, 6555
BveI ACCTGC 1 cut(s) 6297
CaiI CAGNNNCTG 1 cut(s) 9323
CciI TCATGA 5 cut(s) 3352, 4104, 7153, 8220, 9223
CfoI GCGC 4 cut(s) 988, 1445, 9448, 9750
ClaI ATCGAT 2 cut(s) 2348, 5784
CseI GACGC 3 cut(s) 2122, 6227, 10100
CsiI ACCWGGT 2 cut(s) 5758, 8056
CspCI CAANNNNNGTGG 8 cut(s) 463, 498, 1980, 2015, 3313, 3348, 9945, 9980
DraI TTTAAA 6 cut(s) 1812, 3537, 3936, 6432, 6673, 7254
DraIII CACNNNGTG 2 cut(s) 737, 5371
DrdI GACNNNNNNGTC 2 cut(s) 3695, 9567
DriI GACNNNNNGTC 1 cut(s) 9313
DseDI GACNNNNNNGTC 2 cut(s) 3695, 9567
EaeI YGGCCR 7 cut(s) 357, 1498, 2054, 3636, 6213, 8536, 9657
Eam1104I CTCTTC 8 cut(s) 279, 562, 1010, 1246, 2025, 3406, 6899, 7273
Eam1105I GACNNNNNGTC 1 cut(s) 9313
EarI CTCTTC 8 cut(s) 279, 562, 1010, 1246, 2025, 3406, 6899, 7273
EciI GGCGGA 1 cut(s) 7407
Ecl136II GAGCTC 3 cut(s) 2961, 4728, 8936
Eco147I AGGCCT 2 cut(s) 1642, 9772
Eco24I GRGCYC 5 cut(s) 2963, 3509, 4730, 8938, 9347
Eco31I GGTCTC 1 cut(s) 3012
Eco32I GATATC 3 cut(s) 4180, 5013, 5464
Eco47III AGCGCT 1 cut(s) 9749
Eco53kI GAGCTC 3 cut(s) 2961, 4728, 8936
Eco57I CTGAAG 9 cut(s) 2088, 3387, 3809, 4232, 4335, 5175, 6962, 7161, 9158
Eco81I CCTNAGG 2 cut(s) 4137, 8364
Eco88I CYCGRG 2 cut(s) 1715, 3149
Eco91I GGTNACC 2 cut(s) 3116, 5336
EcoICRI GAGCTC 3 cut(s) 2961, 4728, 8936
EcoO109I RGGNCCY 8 cut(s) 226, 1840, 3045, 3505, 3506, 3898, 5162, 8188
EcoO65I GGTNACC 2 cut(s) 3116, 5336
EcoRI GAATTC 2 cut(s) 6103, 9129
EcoRV GATATC 3 cut(s) 4180, 5013, 5464
EcoT22I ATGCAT 7 cut(s) 3368, 5064, 6666, 6826, 7295, 7567, 10053
EcoT38I GRGCYC 5 cut(s) 2963, 3509, 4730, 8938, 9347
Esp3I CGTCTC 2 cut(s) 7822, 8045
FauI CCCGC 1 cut(s) 801
FauNDI CATATG 3 cut(s) 2881, 5629, 6660
FbaI TGATCA 8 cut(s) 1117, 2752, 4107, 4144, 5086, 5544, 6964, 8349
FblI GTMKAC 3 cut(s) 1879, 8245, 10247
FriOI GRGCYC 5 cut(s) 2963, 3509, 4730, 8938, 9347
FspAI RTGCGCAY 1 cut(s) 1444
FspI TGCGCA 3 cut(s) 987, 1444, 9447
GlaI GCGC 4 cut(s) 987, 1444, 9447, 9749
GsaI CCCAGC 4 cut(s) 396, 2369, 3242, 7518
GsuI CTGGAG 7 cut(s) 134, 949, 1320, 2519, 4973, 6283, 6852
HaeII RGCGCY 1 cut(s) 9751
HapII CCGG 5 cut(s) 549, 2890, 4688, 6583, 8111
HgaI GACGC 3 cut(s) 2122, 6227, 10100
HhaI GCGC 4 cut(s) 988, 1445, 9448, 9750
Hin1I GRCGYC 2 cut(s) 6238, 7815
Hin6I GCGC 4 cut(s) 986, 1443, 9446, 9748
HinP1I GCGC 4 cut(s) 986, 1443, 9446, 9748
HincII GTYRAC 9 cut(s) 583, 763, 1081, 1880, 4645, 6628, 7239, 9018, 9147
HindII GTYRAC 9 cut(s) 583, 763, 1081, 1880, 4645, 6628, 7239, 9018, 9147
HindIII AAGCTT 3 cut(s) 3267, 8677, 9025
HpaI GTTAAC 2 cut(s) 583, 1081
HpaII CCGG 5 cut(s) 549, 2890, 4688, 6583, 8111
Hpy99I CGWCG 3 cut(s) 122, 3664, 4943
Hsp92I GRCGYC 2 cut(s) 6238, 7815
HspAI GCGC 4 cut(s) 986, 1443, 9446, 9748
KflI GGGWCCC 2 cut(s) 226, 3898
KpnI GGTACC 1 cut(s) 8057
Ksp22I TGATCA 8 cut(s) 1117, 2752, 4107, 4144, 5086, 5544, 6964, 8349
KspAI GTTAAC 2 cut(s) 583, 1081
LguI GCTCTTC 1 cut(s) 6899
MabI ACCWGGT 2 cut(s) 5758, 8056
MbiI CCGCTC 1 cut(s) 6892
MfeI CAATTG 7 cut(s) 1125, 2573, 6243, 7616, 8928, 9150, 10251
MhlI GDGCHC 9 cut(s) 2392, 2963, 3509, 3786, 4730, 8938, 9127, 9347, 9624
MlsI TGGCCA 5 cut(s) 359, 1500, 3638, 8538, 9659
MluNI TGGCCA 5 cut(s) 359, 1500, 3638, 8538, 9659
Mox20I TGGCCA 5 cut(s) 359, 1500, 3638, 8538, 9659
Mph1103I ATGCAT 7 cut(s) 3368, 5064, 6666, 6826, 7295, 7567, 10053
MroXI GAANNNNTTC 5 cut(s) 2093, 4739, 6424, 6975, 7057
MscI TGGCCA 5 cut(s) 359, 1500, 3638, 8538, 9659
Msp20I TGGCCA 5 cut(s) 359, 1500, 3638, 8538, 9659
MspA1I CMGCKG 8 cut(s) 812, 2115, 5480, 6058, 6643, 8534, 9251, 9668
MspCI CTTAAG 4 cut(s) 4130, 4441, 8680, 9793
MspI CCGG 5 cut(s) 549, 2890, 4688, 6583, 8111
MunI CAATTG 7 cut(s) 1125, 2573, 6243, 7616, 8928, 9150, 10251
Mva1269I GAATGC 3 cut(s) 2615, 2716, 5115
MvnI CGCG 1 cut(s) 3492
NciI CCSGG 2 cut(s) 550, 6583
NcoI CCATGG 5 cut(s) 206, 3702, 5998, 9041, 9270
NdeI CATATG 3 cut(s) 2881, 5629, 6660
NheI GCTAGC 2 cut(s) 9167, 10123
NmeAIII GCCGAG 4 cut(s) 467, 2032, 9190, 9806
NsbI TGCGCA 3 cut(s) 987, 1444, 9447
NsiI ATGCAT 7 cut(s) 3368, 5064, 6666, 6826, 7295, 7567, 10053
NspV TTCGAA 2 cut(s) 6, 6101
OliI CACNNNNGTG 2 cut(s) 1580, 7748
PaeI GCATGC 4 cut(s) 2790, 7293, 7569, 10055
PaeR7I CTCGAG 1 cut(s) 1715
PagI TCATGA 5 cut(s) 3352, 4104, 7153, 8220, 9223
PceI AGGCCT 2 cut(s) 1642, 9772
PciI ACATGT 1 cut(s) 890
PciSI GCTCTTC 1 cut(s) 6899
PcsI WCGNNNNNNNCGW 2 cut(s) 12, 7270
PctI GAATGC 3 cut(s) 2615, 2716, 5115
PdmI GAANNNNTTC 5 cut(s) 2093, 4739, 6424, 6975, 7057
PflMI CCANNNNNTGG 3 cut(s) 2575, 7514, 9590
Ppu21I YACGTR 2 cut(s) 3545, 6698
PpuMI RGGWCCY 5 cut(s) 226, 1840, 3045, 3898, 8188
PscI ACATGT 1 cut(s) 890
PshAI GACNNNNGTC 2 cut(s) 1317, 1885
PshBI ATTAAT 5 cut(s) 3410, 4305, 5709, 6071, 9356
PsiI TTATAA 3 cut(s) 6276, 7587, 7689
Psp124BI GAGCTC 3 cut(s) 2963, 4730, 8938
Psp5II RGGWCCY 5 cut(s) 226, 1840, 3045, 3898, 8188
PspEI GGTNACC 2 cut(s) 3116, 5336
PspFI CCCAGC 4 cut(s) 392, 2365, 3238, 7514
PspOMI GGGCCC 1 cut(s) 3505
PspPPI RGGWCCY 5 cut(s) 226, 1840, 3045, 3898, 8188
PspXI VCTCGAGB 1 cut(s) 1715
PstI CTGCAG 3 cut(s) 349, 1210, 1567
PstNI CAGNNNCTG 1 cut(s) 9323
PvuII CAGCTG 6 cut(s) 2115, 5480, 6643, 8534, 9251, 9668
SacI GAGCTC 3 cut(s) 2963, 4730, 8938
SalI GTCGAC 1 cut(s) 1878
SapI GCTCTTC 1 cut(s) 6899
ScaI AGTACT 4 cut(s) 800, 1138, 6975, 9834
SduI GDGCHC 9 cut(s) 2392, 2963, 3509, 3786, 4730, 8938, 9127, 9347, 9624
SexAI ACCWGGT 2 cut(s) 5758, 8056
SfcI CTRYAG 7 cut(s) 345, 675, 1206, 1563, 1684, 4197, 7912
Sfr274I CTCGAG 1 cut(s) 1715
SfuI TTCGAA 2 cut(s) 6, 6101
SlaI CTCGAG 1 cut(s) 1715
SpeI ACTAGT 2 cut(s) 3299, 9383
SphI GCATGC 4 cut(s) 2790, 7293, 7569, 10055
SseBI AGGCCT 2 cut(s) 1642, 9772
SspI AATATT 9 cut(s) 499, 2122, 2289, 4476, 4738, 6462, 6708, 8356, 10291
SstI GAGCTC 3 cut(s) 2963, 4730, 8938
StuI AGGCCT 2 cut(s) 1642, 9772
TaqII GACCGA 5 cut(s) 32, 748, 3502, 4784, 4791
TatI WGTACW 9 cut(s) 798, 887, 1136, 3839, 5137, 5653, 6973, 8517, 9832
TauI GCSGC 2 cut(s) 7469, 7505
TspGWI ACGGA 9 cut(s) 927, 1027, 1553, 4002, 5601, 7385, 7810, 8784, 9749
Van91I CCANNNNNTGG 3 cut(s) 2575, 7514, 9590
Vha464I CTTAAG 4 cut(s) 4130, 4441, 8680, 9793
VneI GTGCAC 1 cut(s) 9123
VspI ATTAAT 5 cut(s) 3410, 4305, 5709, 6071, 9356
XcmI CCANNNNNNNNNTGG 4 cut(s) 2494, 2650, 5858, 7389
XhoI CTCGAG 1 cut(s) 1715
XmaJI CCTAGG 2 cut(s) 3389, 4027
XmiI GTMKAC 3 cut(s) 1879, 8245, 10247
XmnI GAANNNNTTC 5 cut(s) 2093, 4739, 6424, 6975, 7057
ZraI GACGTC 1 cut(s) 7816
ZrmI AGTACT 4 cut(s) 800, 1138, 6975, 9834
Zsp2I ATGCAT 7 cut(s) 3368, 5064, 6666, 6826, 7295, 7567, 10053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.