RchiOBHm_Chr2g0148301

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001657
Physical Location & Seq
Forward (+)
65923835 .. 65929978
6144 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51788

Sequence Viewer

Length: 954 bp
ATGTTCGAAGGACTCGTCAGCCAGATTCTTCACGGCTATCTCGGTCGCTATGTCAAGGATTTTCACAAGCACCAGCTCAAGATGGAGATTTGGAATGAGGAAGTGTTCTTGGAAAACGTCGAGCTTAGTCTCGAAGCCTTTGATTATCTCCAGTTGCCTTTCGCTCTCAAACAAGGTCGGATTGGAAAGATAAGCATCAAAGTTCCATGGAAAACGCTTGGTGGGGACCCCTTTGTAATTAGTTTGGAAAATGTCTTCCTATGTGCATCCCAACGAGATGATGAAGAGTGGAGTTTGGATGAAGTTGAAAAACGAGAGTTTGCTGGAAAAAAGGCCAAACTTGCTGCAGCAGAACTGGCCAAATTATCAAAATGTGTATGCGAAAACCAGGCTGGGCTTAGATCAATAATTACTGCTAAGGTTCTTGACAGCATCCAAGTGTCAATCAGAGATTTTCATATCTTGTACCATGACAAGCTCAGTGGCTCGGCATGTAATATTTTTGGTTTGAAGATTTCCAGCTTGAGGACTATGAAGCAAAATCCTTCCGGGTCACTTGCAAAGGGAAGAGGGGGCCAAGTTAACAAAACTGTGGAGATTATGGGTTTGGAGTTTTACTGTGGCACACTTGATGGGGCGGTGGAGTTGATGGCTATGAACAATGATGGGGACTCCGTAGTACGGCAAGATATAAGATATGACGGGAAAAGATATAACAACTCCATACTGTCACCATGTGATGTTTCCATGTCACTCTCGGTCAACAGATCAGGAGAGCTTGATAGCAAAATCCCGCAGTACTCCATCACCGCTGAGATAACTGACTTGGTGATGTGCTTAGATGAGGTTCAGTTGCAACAGATTTTGTTTCTGTGGGATTATTTTTGTACATGTGAATTGAGAAAGAAGTATGGGCGTTATCGTCCCTGGAGTAGTCCGTTATCAAAAAGGTGA

Protein Analysis

317

Amino Acids

35.93

Weight (kDa)

6.55

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_N PF12624 1 - 311 4.1e-50 VPS13-like family N-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 638, 794, 810
AcoI YGGCCR 1 cut(s) 357
AdeI CACNNNGTG 1 cut(s) 737
AfaI GTAC 4 cut(s) 467, 681, 800, 889
AfiI CCNNNNNNNGG 2 cut(s) 525, 681
AflIII ACRYGT 1 cut(s) 890
AgsI TTSAA 2 cut(s) 308, 511
AjnI CCWGG 2 cut(s) 387, 926
AluBI AGCT 5 cut(s) 76, 124, 478, 522, 778
AluI AGCT 5 cut(s) 76, 124, 478, 522, 778
Alw26I GTCTC 1 cut(s) 134
AoxI GGCC 3 cut(s) 333, 357, 574
ApeKI GCWGC 2 cut(s) 344, 347
ArsI GACNNNNNNTTYG 1 cut(s) 32
AspS9I GGNCC 2 cut(s) 226, 574
AsuC2I CCSGG 1 cut(s) 550
AsuHPI GGTGA 3 cut(s) 723, 799, 841
AsuII TTCGAA 1 cut(s) 6
AvaII GGWCC 1 cut(s) 226
BalI TGGCCA 1 cut(s) 359
BbsI GAAGAC 1 cut(s) 247
BbvI GCAGC 2 cut(s) 331, 359
BccI CCATC 5 cut(s) 76, 626, 643, 659, 812
BceAI ACGGC 2 cut(s) 49, 698
BciT130I CCWGG 2 cut(s) 389, 928
BcnI CCSGG 1 cut(s) 550
BcoDI GTCTC 1 cut(s) 134
BfmI CTRYAG 1 cut(s) 345
BisI GCNGC 2 cut(s) 345, 348
BlsI GCNGC 2 cut(s) 346, 349
BmcAI AGTACT 1 cut(s) 800
Bme1390I CCNGG 3 cut(s) 389, 550, 928
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 2 cut(s) 226, 574
BmiI GGNNCC 3 cut(s) 227, 228, 575
BmrFI CCNGG 3 cut(s) 389, 550, 928
BmsI GCATC 3 cut(s) 204, 275, 441
BpiI GAAGAC 1 cut(s) 247
BpmI CTGGAG 2 cut(s) 134, 949
Bpu10I CCTNAGC 1 cut(s) 417
Bpu14I TTCGAA 1 cut(s) 6
BpuEI CTTGAG 2 cut(s) 62, 544
BpuMI CCSGG 1 cut(s) 550
BsaBI GATNNNNATC 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 206, 926
Bsc4I CCNNNNNNNGG 2 cut(s) 525, 681
Bse1I ACTGG 2 cut(s) 151, 360
Bse8I GATNNNNATC 1 cut(s) 194
BseBI CCWGG 2 cut(s) 389, 928
BseDI CCNNGG 2 cut(s) 206, 926
BseGI GGATG 3 cut(s) 266, 304, 432
BseJI GATNNNNATC 1 cut(s) 194
BseLI CCNNNNNNNGG 2 cut(s) 525, 681
BseMII CTCAG 2 cut(s) 493, 804
BseNI ACTGG 2 cut(s) 151, 360
BseXI GCAGC 2 cut(s) 331, 359
BseYI CCCAGC 1 cut(s) 392
Bsh1285I CGRYCG 1 cut(s) 46
BshFI GGCC 3 cut(s) 335, 359, 576
BsiEI CGRYCG 1 cut(s) 46
BsiSI CCGG 1 cut(s) 549
BslFI GGGAC 3 cut(s) 239, 683, 909
BslI CCNNNNNNNGG 2 cut(s) 525, 681
BsmAI GTCTC 1 cut(s) 134
BsmFI GGGAC 3 cut(s) 239, 683, 909
BsnI GGCC 3 cut(s) 335, 359, 576
Bsp119I TTCGAA 1 cut(s) 6
Bsp1407I TGTACA 1 cut(s) 887
Bsp143I GATC 2 cut(s) 401, 767
Bsp19I CCATGG 1 cut(s) 206
BspACI CCGC 3 cut(s) 638, 794, 810
BspANI GGCC 3 cut(s) 335, 359, 576
BspCNI CTCAG 2 cut(s) 492, 805
BspLI GGNNCC 3 cut(s) 227, 228, 575
BspMAI CTGCAG 1 cut(s) 349
BspT104I TTCGAA 1 cut(s) 6
BsrGI TGTACA 1 cut(s) 887
BsrI ACTGG 2 cut(s) 151, 360
BssECI CCNNGG 2 cut(s) 206, 926
BssMI GATC 2 cut(s) 401, 767
BssT1I CCWWGG 1 cut(s) 206
Bst2UI CCWGG 2 cut(s) 389, 928
Bst4CI ACNGT 3 cut(s) 592, 620, 729
Bst6I CTCTTC 2 cut(s) 279, 562
BstAUI TGTACA 1 cut(s) 887
BstBI TTCGAA 1 cut(s) 6
BstDEI CTNAG 6 cut(s) 125, 398, 417, 479, 813, 838
BstDSI CCRYGG 1 cut(s) 206
BstF5I GGATG 3 cut(s) 266, 304, 432
BstKTI GATC 2 cut(s) 404, 770
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 2 cut(s) 401, 767
BstMCI CGRYCG 1 cut(s) 46
BstMWI GCNNNNNNNGC 2 cut(s) 341, 356
BstNI CCWGG 2 cut(s) 389, 928
BstNSI RCATGY 2 cut(s) 495, 894
BstSCI CCNGG 3 cut(s) 387, 548, 926
BstSFI CTRYAG 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 331, 359
BstV2I GAAGAC 1 cut(s) 247
BsuRI GGCC 3 cut(s) 335, 359, 576
BtgI CCRYGG 1 cut(s) 206
BtsCI GGATG 3 cut(s) 266, 304, 432
BtsIMutI CAGTG 1 cut(s) 487
Cfr13I GGNCC 2 cut(s) 226, 574
Csp6I GTAC 4 cut(s) 466, 680, 799, 888
CspCI CAANNNNNGTGG 2 cut(s) 463, 498
CviAII CATG 6 cut(s) 207, 470, 492, 735, 748, 891
CviQI GTAC 4 cut(s) 466, 680, 799, 888
DdeI CTNAG 6 cut(s) 125, 398, 417, 479, 813, 838
DpnI GATC 2 cut(s) 403, 769
DpnII GATC 2 cut(s) 401, 767
DraIII CACNNNGTG 1 cut(s) 737
EaeI YGGCCR 1 cut(s) 357
Eam1104I CTCTTC 2 cut(s) 279, 562
EarI CTCTTC 2 cut(s) 279, 562
Eco130I CCWWGG 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 226
EcoRII CCWGG 2 cut(s) 387, 926
EcoT14I CCWWGG 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 206
FaeI CATG 6 cut(s) 210, 473, 495, 738, 751, 894
FaqI GGGAC 3 cut(s) 239, 683, 909
FatI CATG 6 cut(s) 206, 469, 491, 734, 747, 890
FauI CCCGC 1 cut(s) 801
Fnu4HI GCNGC 2 cut(s) 345, 348
FokI GGATG 3 cut(s) 253, 311, 419
Fsp4HI GCNGC 2 cut(s) 345, 348
GluI GCNGC 2 cut(s) 345, 348
GsaI CCCAGC 1 cut(s) 396
GsuI CTGGAG 2 cut(s) 134, 949
HaeIII GGCC 3 cut(s) 335, 359, 576
HapII CCGG 1 cut(s) 549
Hin1II CATG 6 cut(s) 210, 473, 495, 738, 751, 894
HincII GTYRAC 2 cut(s) 583, 763
HindII GTYRAC 2 cut(s) 583, 763
HinfI GANTC 3 cut(s) 12, 25, 671
HpaI GTTAAC 1 cut(s) 583
HpaII CCGG 1 cut(s) 549
HphI GGTGA 3 cut(s) 723, 799, 841
Hpy166II GTNNAC 2 cut(s) 583, 763
Hpy188I TCNGA 2 cut(s) 180, 449
Hpy188III TCNNGA 4 cut(s) 79, 131, 425, 771
Hpy8I GTNNAC 2 cut(s) 583, 763
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 1 cut(s) 555
HpyCH4III ACNGT 3 cut(s) 592, 620, 729
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 4 cut(s) 266, 347, 560, 856
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 356
HpyF3I CTNAG 6 cut(s) 125, 398, 417, 479, 813, 838
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 6 cut(s) 210, 473, 495, 738, 751, 894
KflI GGGWCCC 1 cut(s) 226
KspAI GTTAAC 1 cut(s) 583
Kzo9I GATC 2 cut(s) 401, 767
Lsp1109I GCAGC 2 cut(s) 331, 359
LweI GCATC 3 cut(s) 204, 275, 441
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 3 cut(s) 552, 729, 750
MalI GATC 2 cut(s) 403, 769
MboI GATC 2 cut(s) 401, 767
MboII GAAGA 5 cut(s) 20, 247, 296, 523, 579
MlsI TGGCCA 1 cut(s) 359
MluCI AATT 4 cut(s) 237, 362, 408, 896
MluNI TGGCCA 1 cut(s) 359
MlyI GAGTC 2 cut(s) 6, 665
MmeI TCCRAC 1 cut(s) 158
MnlI CCTC 4 cut(s) 91, 519, 563, 838
Mox20I TGGCCA 1 cut(s) 359
MscI TGGCCA 1 cut(s) 359
MseI TTAA 1 cut(s) 582
MslI CAYNNNNRTG 1 cut(s) 437
Msp20I TGGCCA 1 cut(s) 359
MspA1I CMGCKG 1 cut(s) 812
MspI CCGG 1 cut(s) 549
MspR9I CCNGG 3 cut(s) 389, 550, 928
MvaI CCWGG 2 cut(s) 389, 928
MwoI GCNNNNNNNGC 2 cut(s) 341, 356
NciI CCSGG 1 cut(s) 550
NcoI CCATGG 1 cut(s) 206
NdeII GATC 2 cut(s) 401, 767
NlaIII CATG 6 cut(s) 210, 473, 495, 738, 751, 894
NlaIV GGNNCC 3 cut(s) 227, 228, 575
NmeAIII GCCGAG 1 cut(s) 467
NmuCI GTSAC 3 cut(s) 552, 729, 750
NspI RCATGY 2 cut(s) 495, 894
NspV TTCGAA 1 cut(s) 6
PciI ACATGT 1 cut(s) 890
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PfeI GAWTC 1 cut(s) 25
PkrI GCNGC 2 cut(s) 346, 349
PleI GAGTC 2 cut(s) 6, 665
PpsI GAGTC 2 cut(s) 6, 665
PpuMI RGGWCCY 1 cut(s) 226
PscI ACATGT 1 cut(s) 890
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 2 cut(s) 387, 926
PspFI CCCAGC 1 cut(s) 392
PspGI CCWGG 2 cut(s) 387, 926
PspN4I GGNNCC 3 cut(s) 227, 228, 575
PspPI GGNCC 2 cut(s) 226, 574
PspPPI RGGWCCY 1 cut(s) 226
PstI CTGCAG 1 cut(s) 349
RsaI GTAC 4 cut(s) 467, 681, 800, 889
RsaNI GTAC 4 cut(s) 466, 680, 799, 888
RseI CAYNNNNRTG 1 cut(s) 437
SaqAI TTAA 1 cut(s) 582
SatI GCNGC 2 cut(s) 345, 348
Sau3AI GATC 2 cut(s) 401, 767
Sau96I GGNCC 2 cut(s) 226, 574
ScaI AGTACT 1 cut(s) 800
SchI GAGTC 2 cut(s) 6, 665
ScrFI CCNGG 3 cut(s) 389, 550, 928
SfaNI GCATC 3 cut(s) 204, 275, 441
SfcI CTRYAG 1 cut(s) 345
SfuI TTCGAA 1 cut(s) 6
SinI GGWCC 1 cut(s) 226
SmiMI CAYNNNNRTG 1 cut(s) 437
SmlI CTYRAG 2 cut(s) 77, 523
SmoI CTYRAG 2 cut(s) 77, 523
Sse9I AATT 4 cut(s) 237, 362, 408, 896
SsiI CCGC 3 cut(s) 638, 794, 810
SspI AATATT 1 cut(s) 499
StyD4I CCNGG 3 cut(s) 387, 548, 926
StyI CCWWGG 1 cut(s) 206
TaaI ACNGT 3 cut(s) 592, 620, 729
TaiI ACGT 1 cut(s) 120
TaqI TCGA 3 cut(s) 6, 120, 132
TaqII GACCGA 2 cut(s) 32, 748
TasI AATT 4 cut(s) 237, 362, 408, 896
TatI WGTACW 2 cut(s) 798, 887
TfiI GAWTC 1 cut(s) 25
Tru1I TTAA 1 cut(s) 582
Tru9I TTAA 1 cut(s) 582
TscAI CASTG 1 cut(s) 487
TseFI GTSAC 3 cut(s) 552, 729, 750
TseI GCWGC 2 cut(s) 344, 347
Tsp45I GTSAC 3 cut(s) 552, 729, 750
TspDTI ATGAA 5 cut(s) 297, 315, 446, 548, 671
TspGWI ACGGA 2 cut(s) 664, 927
TspRI CASTG 1 cut(s) 487
VpaK11BI GGWCC 1 cut(s) 226
XceI RCATGY 2 cut(s) 495, 894
ZrmI AGTACT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.