FvH4_6g37711

V-type proton ATPase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005434
Physical Location & Seq
Reverse (-)
29715680 .. 29718071
2392 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g37711.t2

Sequence Viewer

Length: 786 bp
ATGTCGGGGCAAAGCCAGCGTTTGAATGTGGTTCCTACTGTTACAATGCTTGGGATTATGAAAGCTCGTCTTATTGGGGCGACTAGAGGTCATGCTCTCCTCAAGAAGAAGAGTGATGCTCTAACTGTTCAGTTTCGTGCCATTCTTAAAAAGATTGTGTCTACCAAAGAATCAATGGGTGATATTATGAAAACCTCTTCCTTTGCTCTTACTGAAGCCAAGTATGTTGCTGGTGAGAACATCAAACACATTGTTCTCGAGAATGTCAACACTGCCTCTCTTAAAGTTCGATCAAAGCAAGAGAATGTTGCTGGTGTAAAGCTCCCAAAATTTGAGTACTTTACTGAAGGTGAGACCAAGAATGATCTCACTGGATTGGCCAGAGGTGGACAACAGGTTCAACTGTGCAAGGCTGCTTATGTGAAAGCAATTGAGGTTCTTGTGGAGCTTGCTTCACTTCAAACATCATTTTTAACACTTGATGAAGCAATCAAGAGCACAAATCGCAGGGTCAATGCTCTGGAGAATGTGGTGAAGCCTAGGTTGGAGAATACGATAACTTATATCAAGGGAGAGTTGGATGAGCTGGAAAGGGAGGACTTCTTCAGGTTAAAGAAGATACAGGGATTTAAGAAGAGGGAAATTGAGAGACAGATGGCAGCTGCTAAGAACTTCTCGGAAGAACAGGCTGCAGAAGTTTTGTCTTTAAAGAAGGGTATTTCACTAAATTCAGCTCAGAATTTGTTGTCTGGAGGTATGGGAAAGGACGAGGATATCATTTTCTGA

Protein Analysis

262

Amino Acids

28.94

Weight (kDa)

9.54

Isoelectric Point (pI)

28.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_D PF01813 17 - 208 1.2e-67 ATP synthase subunit D
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 161
AcoI YGGCCR 1 cut(s) 378
AcsI RAATTY 3 cut(s) 329, 727, 739
AcuI CTGAAG 3 cut(s) 234, 366, 589
AfaI GTAC 1 cut(s) 338
AgsI TTSAA 3 cut(s) 25, 401, 461
AhdI GACNNNNNGTC 1 cut(s) 87
AjuI GAANNNNNNNTTGG 2 cut(s) 527, 559
AloI GAACNNNNNNTCC 2 cut(s) 381, 413
AluBI AGCT 6 cut(s) 65, 322, 448, 586, 662, 734
AluI AGCT 6 cut(s) 65, 322, 448, 586, 662, 734
Alw21I GWGCWC 1 cut(s) 500
Alw26I GTCTC 2 cut(s) 347, 643
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 1 cut(s) 378
ApeKI GCWGC 4 cut(s) 413, 659, 662, 689
ApoI RAATTY 3 cut(s) 329, 727, 739
AspA2I CCTAGG 1 cut(s) 539
AsuHPI GGTGA 4 cut(s) 191, 245, 362, 544
AvaI CYCGRG 1 cut(s) 257
AvrII CCTAGG 1 cut(s) 539
BalI TGGCCA 1 cut(s) 380
Bbv12I GWGCWC 1 cut(s) 500
BbvI GCAGC 4 cut(s) 400, 649, 671, 676
BccI CCATC 1 cut(s) 649
BcoDI GTCTC 2 cut(s) 347, 643
BfaI CTAG 2 cut(s) 84, 540
BfmI CTRYAG 1 cut(s) 690
BisI GCNGC 4 cut(s) 414, 660, 663, 690
BlnI CCTAGG 1 cut(s) 539
BlsI GCNGC 4 cut(s) 415, 661, 664, 691
BmcAI AGTACT 1 cut(s) 338
BmeRI GACNNNNNGTC 1 cut(s) 87
BmeT110I CYCGRG 1 cut(s) 257
BmiI GGNNCC 1 cut(s) 33
BmsI GCATC 1 cut(s) 106
BplI GAGNNNNNCTC 2 cut(s) 103, 135
BpmI CTGGAG 2 cut(s) 542, 771
BpuEI CTTGAG 1 cut(s) 86
BsaI GGTCTC 1 cut(s) 347
BsaJI CCNNGG 1 cut(s) 539
Bse1I ACTGG 1 cut(s) 376
BseDI CCNNGG 1 cut(s) 539
BseGI GGATG 1 cut(s) 586
BseMII CTCAG 1 cut(s) 749
BseNI ACTGG 1 cut(s) 376
BseRI GAGGAG 1 cut(s) 89
BseXI GCAGC 4 cut(s) 400, 649, 671, 676
BshFI GGCC 1 cut(s) 380
BsiHKAI GWGCWC 1 cut(s) 500
BsiHKCI CYCGRG 1 cut(s) 257
BsmAI GTCTC 2 cut(s) 347, 643
BsnI GGCC 1 cut(s) 380
Bso31I GGTCTC 1 cut(s) 347
BsoBI CYCGRG 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 500
Bsp143I GATC 2 cut(s) 290, 364
BspANI GGCC 1 cut(s) 380
BspCNI CTCAG 1 cut(s) 748
BspLI GGNNCC 1 cut(s) 33
BspMAI CTGCAG 1 cut(s) 694
BspTNI GGTCTC 1 cut(s) 347
BsrI ACTGG 1 cut(s) 376
BssECI CCNNGG 1 cut(s) 539
BssMI GATC 2 cut(s) 290, 364
BssT1I CCWWGG 1 cut(s) 539
Bst4CI ACNGT 3 cut(s) 40, 127, 405
Bst6I CTCTTC 3 cut(s) 104, 202, 629
BstC8I GCNNGC 2 cut(s) 17, 450
BstDEI CTNAG 2 cut(s) 666, 735
BstF5I GGATG 1 cut(s) 586
BstKTI GATC 2 cut(s) 293, 367
BstMAI GTCTC 2 cut(s) 347, 643
BstMBI GATC 2 cut(s) 290, 364
BstMWI GCNNNNNNNGC 2 cut(s) 16, 504
BstSFI CTRYAG 1 cut(s) 690
BstV1I GCAGC 4 cut(s) 400, 649, 671, 676
BsuRI GGCC 1 cut(s) 380
BtsCI GGATG 1 cut(s) 586
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 2 cut(s) 270, 369
Cac8I GCNNGC 2 cut(s) 17, 450
Csp6I GTAC 1 cut(s) 337
CviAII CATG 1 cut(s) 92
CviQI GTAC 1 cut(s) 337
DdeI CTNAG 2 cut(s) 666, 735
DpnI GATC 2 cut(s) 292, 366
DpnII GATC 2 cut(s) 290, 364
DraI TTTAAA 1 cut(s) 708
DriI GACNNNNNGTC 1 cut(s) 87
EaeI YGGCCR 1 cut(s) 378
Eam1104I CTCTTC 3 cut(s) 104, 202, 629
Eam1105I GACNNNNNGTC 1 cut(s) 87
EarI CTCTTC 3 cut(s) 104, 202, 629
Eco130I CCWWGG 1 cut(s) 539
Eco31I GGTCTC 1 cut(s) 347
Eco32I GATATC 1 cut(s) 775
Eco57I CTGAAG 3 cut(s) 234, 366, 589
Eco88I CYCGRG 1 cut(s) 257
EcoRV GATATC 1 cut(s) 775
EcoT14I CCWWGG 1 cut(s) 539
ErhI CCWWGG 1 cut(s) 539
FaeI CATG 1 cut(s) 95
FaiI YATR 7 cut(s) 59, 93, 188, 225, 420, 564, 758
FalI AAGNNNNNCTT 2 cut(s) 54, 86
FatI CATG 1 cut(s) 91
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 4 cut(s) 414, 660, 663, 690
FokI GGATG 1 cut(s) 593
Fsp4HI GCNGC 4 cut(s) 414, 660, 663, 690
FspBI CTAG 2 cut(s) 84, 540
GluI GCNGC 4 cut(s) 414, 660, 663, 690
GsuI CTGGAG 2 cut(s) 542, 771
HaeIII GGCC 1 cut(s) 380
Hin1II CATG 1 cut(s) 95
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 1 cut(s) 170
HphI GGTGA 4 cut(s) 191, 245, 362, 544
Hpy166II GTNNAC 3 cut(s) 162, 268, 389
Hpy188I TCNGA 3 cut(s) 679, 738, 785
Hpy188III TCNNGA 6 cut(s) 103, 257, 259, 493, 521, 750
Hpy8I GTNNAC 3 cut(s) 162, 268, 389
HpyAV CCTTC 2 cut(s) 341, 706
HpyCH4III ACNGT 3 cut(s) 40, 127, 405
HpyCH4V TGCA 2 cut(s) 408, 692
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 504
HpyF3I CTNAG 2 cut(s) 666, 735
Hsp92II CATG 1 cut(s) 95
Kzo9I GATC 2 cut(s) 290, 364
LmnI GCTCC 2 cut(s) 327, 445
Lsp1109I GCAGC 4 cut(s) 400, 649, 671, 676
LweI GCATC 1 cut(s) 106
MaeI CTAG 2 cut(s) 84, 540
MaeIII GTNAC 1 cut(s) 40
MalI GATC 2 cut(s) 292, 366
MboI GATC 2 cut(s) 290, 364
MboII GAAGA 7 cut(s) 118, 121, 189, 595, 628, 646, 692
MfeI CAATTG 1 cut(s) 429
MhlI GDGCHC 1 cut(s) 500
MlsI TGGCCA 1 cut(s) 380
MluCI AATT 5 cut(s) 329, 429, 642, 727, 739
MluNI TGGCCA 1 cut(s) 380
MmeI TCCRAC 2 cut(s) 525, 558
Mox20I TGGCCA 1 cut(s) 380
MscI TGGCCA 1 cut(s) 380
MseI TTAA 6 cut(s) 147, 282, 473, 611, 630, 707
Msp20I TGGCCA 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 662
MunI CAATTG 1 cut(s) 429
MwoI GCNNNNNNNGC 2 cut(s) 16, 504
NdeII GATC 2 cut(s) 290, 364
NlaIII CATG 1 cut(s) 95
NlaIV GGNNCC 1 cut(s) 33
PaeR7I CTCGAG 1 cut(s) 257
PfeI GAWTC 1 cut(s) 170
PkrI GCNGC 4 cut(s) 415, 661, 664, 691
PspN4I GGNNCC 1 cut(s) 33
PstI CTGCAG 1 cut(s) 694
PvuII CAGCTG 1 cut(s) 662
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
SaqAI TTAA 6 cut(s) 147, 282, 473, 611, 630, 707
SatI GCNGC 4 cut(s) 414, 660, 663, 690
Sau3AI GATC 2 cut(s) 290, 364
ScaI AGTACT 1 cut(s) 338
SduI GDGCHC 1 cut(s) 500
SfaNI GCATC 1 cut(s) 106
SfcI CTRYAG 1 cut(s) 690
Sfr274I CTCGAG 1 cut(s) 257
SlaI CTCGAG 1 cut(s) 257
SmlI CTYRAG 2 cut(s) 101, 257
SmoI CTYRAG 2 cut(s) 101, 257
Sse9I AATT 5 cut(s) 329, 429, 642, 727, 739
SspMI CTAG 2 cut(s) 84, 540
StyI CCWWGG 1 cut(s) 539
TaaI ACNGT 3 cut(s) 40, 127, 405
TaqI TCGA 2 cut(s) 258, 289
TasI AATT 5 cut(s) 329, 429, 642, 727, 739
TatI WGTACW 1 cut(s) 336
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 6 cut(s) 147, 282, 473, 611, 630, 707
Tru9I TTAA 6 cut(s) 147, 282, 473, 611, 630, 707
TscAI CASTG 2 cut(s) 277, 376
TseI GCWGC 4 cut(s) 413, 659, 662, 689
TspDTI ATGAA 3 cut(s) 74, 203, 498
TspRI CASTG 2 cut(s) 277, 376
XapI RAATTY 3 cut(s) 329, 727, 739
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XhoI CTCGAG 1 cut(s) 257
XmaJI CCTAGG 1 cut(s) 539
XmiI GTMKAC 1 cut(s) 161
XspI CTAG 2 cut(s) 84, 540
ZrmI AGTACT 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.