MD17G1143000.v1.1

V-type proton ATPase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005434
Physical Location & Seq
Forward (+)
12892447 .. 12894822
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1143000.v1.1.491

Sequence Viewer

Length: 735 bp
ATGCTTGGAGTTATGAAAGCTCGCCTTGTAGGTGCGACAAGAGGTCATGCTCTCCTCAAGAAGAAGAGCGATGCTTTAACAGTGCAGTTCCGTCAGATTCTTAAAAATATTGTCGCAACCAAAGAATCCATGGGAGATGTGATGAAGAACTCCTCCTTTGCCCTTACTGAAGCCAAGTATGTTGCTGGCGAGAACATCAAGCACATTGTTCTTGAGAATGTCCACAATGCCTCTATTAAAGTTCGATCAAGGCAAGAGAATGTTGCTGGTGTAAAGCTCCCAAAGTTTGAATACTTTACTGAAGGTGAGACCAAGAATGACCTCACTGGATTGGCAAGAGGCGGACAACAGGTCCAACTCTGTCGTGCTGCTTATGTGAAAGCAATTGAGGTTCTCGTGGAGCTTGCTTCACTTCAAACATCATTTTTAACCCTTGATGAAGCAATCAAGACCACAAATCGTCGTGTCAATGCCCTAGAGAATGTTGTGAAGCCTAGGTTGGAGAACACAATAAGCTATATCAAGGGGGAGCTGGATGAGCTCGAAAGAGAGGATTTCTTCAGGTTAAAGAAGATACAGGGTTATAAGAAGAGGGAAATCGAGAGACAGATGGCAGCTGCCAGGAATTTTGCCAACGAGCAGGTTGCGGAAAAGTTATCGCTGAAGAAGGGCATTTCCCTACATTCAGCTCAGAACTTGTTGTCTGCGGAAAAGGATGACGACATCATTTTCTGA

Protein Analysis

245

Amino Acids

27.41

Weight (kDa)

9.5

Isoelectric Point (pI)

27.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_D PF01813 2 - 193 4.6e-69 ATP synthase subunit D
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
Acc36I ACCTGC 1 cut(s) 631
AciI CCGC 3 cut(s) 342, 647, 707
AcsI RAATTY 1 cut(s) 625
AcuI CTGAAG 4 cut(s) 189, 321, 544, 683
AgsI TTSAA 2 cut(s) 290, 416
AhdI GACNNNNNGTC 2 cut(s) 42, 350
AjnI CCWGG 1 cut(s) 620
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AluBI AGCT 8 cut(s) 20, 277, 403, 516, 532, 541, 617, 689
AluI AGCT 8 cut(s) 20, 277, 403, 516, 532, 541, 617, 689
Alw21I GWGCWC 1 cut(s) 543
Alw26I GTCTC 2 cut(s) 302, 598
ApeKI GCWGC 3 cut(s) 368, 614, 617
ApoI RAATTY 1 cut(s) 625
AspA2I CCTAGG 1 cut(s) 494
AspS9I GGNCC 1 cut(s) 352
AsuHPI GGTGA 1 cut(s) 317
AvaII GGWCC 1 cut(s) 352
AvrII CCTAGG 1 cut(s) 494
BanII GRGCYC 1 cut(s) 543
BauI CACGAG 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 543
BbvI GCAGC 3 cut(s) 355, 604, 626
BccI CCATC 1 cut(s) 604
BcgI CGANNNNNNTGC 2 cut(s) 626, 660
BciT130I CCWGG 1 cut(s) 622
BcoDI GTCTC 2 cut(s) 302, 598
BfaI CTAG 2 cut(s) 476, 495
BfuAI ACCTGC 1 cut(s) 631
BisI GCNGC 3 cut(s) 369, 615, 618
BlnI CCTAGG 1 cut(s) 494
BlsI GCNGC 3 cut(s) 370, 616, 619
Bme1390I CCNGG 1 cut(s) 622
Bme18I GGWCC 1 cut(s) 352
BmeRI GACNNNNNGTC 2 cut(s) 42, 350
BmgT120I GGNCC 1 cut(s) 352
BmrFI CCNGG 1 cut(s) 622
BmsI GCATC 1 cut(s) 61
BpuEI CTTGAG 2 cut(s) 41, 233
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 2 cut(s) 129, 494
Bse1I ACTGG 1 cut(s) 331
BseBI CCWGG 1 cut(s) 622
BseDI CCNNGG 2 cut(s) 129, 494
BseGI GGATG 2 cut(s) 541, 721
BseMII CTCAG 1 cut(s) 704
BseNI ACTGG 1 cut(s) 331
BseRI GAGGAG 2 cut(s) 44, 142
BseXI GCAGC 3 cut(s) 355, 604, 626
BsgI GTGCAG 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 543
BsmAI GTCTC 2 cut(s) 302, 598
Bso31I GGTCTC 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 543
Bsp143I GATC 1 cut(s) 245
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 3 cut(s) 342, 647, 707
BspCNI CTCAG 1 cut(s) 703
BspMI ACCTGC 1 cut(s) 631
BspQI GCTCTTC 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 302
BsrI ACTGG 1 cut(s) 331
BssECI CCNNGG 2 cut(s) 129, 494
BssMI GATC 1 cut(s) 245
BssSI CACGAG 1 cut(s) 395
BssT1I CCWWGG 2 cut(s) 129, 494
Bst2BI CACGAG 1 cut(s) 395
Bst2UI CCWGG 1 cut(s) 622
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 2 cut(s) 59, 584
BstC8I GCNNGC 3 cut(s) 22, 187, 405
BstDEI CTNAG 1 cut(s) 690
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 2 cut(s) 541, 721
BstKTI GATC 1 cut(s) 248
BstMAI GTCTC 2 cut(s) 302, 598
BstMBI GATC 1 cut(s) 245
BstMWI GCNNNNNNNGC 1 cut(s) 538
BstNI CCWGG 1 cut(s) 622
BstSCI CCNGG 1 cut(s) 620
BstV1I GCAGC 3 cut(s) 355, 604, 626
BtgI CCRYGG 1 cut(s) 129
BtgZI GCGATG 1 cut(s) 84
BtsCI GGATG 2 cut(s) 541, 721
BtsIMutI CAGTG 2 cut(s) 87, 324
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 3 cut(s) 22, 187, 405
Cfr13I GGNCC 1 cut(s) 352
CviAII CATG 2 cut(s) 47, 130
DdeI CTNAG 1 cut(s) 690
DpnI GATC 1 cut(s) 247
DpnII GATC 1 cut(s) 245
DriI GACNNNNNGTC 2 cut(s) 42, 350
Eam1104I CTCTTC 2 cut(s) 59, 584
Eam1105I GACNNNNNGTC 2 cut(s) 42, 350
EarI CTCTTC 2 cut(s) 59, 584
EciI GGCGGA 1 cut(s) 357
Ecl136II GAGCTC 1 cut(s) 541
Eco130I CCWWGG 2 cut(s) 129, 494
Eco24I GRGCYC 1 cut(s) 543
Eco31I GGTCTC 1 cut(s) 302
Eco47I GGWCC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 541
Eco57I CTGAAG 4 cut(s) 189, 321, 544, 683
EcoICRI GAGCTC 1 cut(s) 541
EcoRII CCWGG 1 cut(s) 620
EcoT14I CCWWGG 2 cut(s) 129, 494
EcoT38I GRGCYC 1 cut(s) 543
ErhI CCWWGG 2 cut(s) 129, 494
FaeI CATG 2 cut(s) 50, 133
FaiI YATR 7 cut(s) 14, 48, 131, 180, 375, 519, 585
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FatI CATG 2 cut(s) 46, 129
Fnu4HI GCNGC 3 cut(s) 369, 615, 618
FokI GGATG 2 cut(s) 548, 728
FriOI GRGCYC 1 cut(s) 543
Fsp4HI GCNGC 3 cut(s) 369, 615, 618
FspBI CTAG 2 cut(s) 476, 495
GluI GCNGC 3 cut(s) 369, 615, 618
Hin1II CATG 2 cut(s) 50, 133
HinfI GANTC 2 cut(s) 97, 125
HphI GGTGA 1 cut(s) 317
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 3 cut(s) 96, 693, 734
Hpy188III TCNNGA 4 cut(s) 58, 212, 448, 601
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 1 cut(s) 465
HpyAV CCTTC 2 cut(s) 296, 661
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4V TGCA 1 cut(s) 85
HpyF10VI GCNNNNNNNGC 1 cut(s) 538
HpyF3I CTNAG 1 cut(s) 690
Hsp92II CATG 2 cut(s) 50, 133
Kzo9I GATC 1 cut(s) 245
LguI GCTCTTC 1 cut(s) 59
LmnI GCTCC 3 cut(s) 282, 400, 529
Lsp1109I GCAGC 3 cut(s) 355, 604, 626
LweI GCATC 1 cut(s) 61
MaeI CTAG 2 cut(s) 476, 495
MalI GATC 1 cut(s) 247
MboI GATC 1 cut(s) 245
MboII GAAGA 7 cut(s) 73, 76, 157, 550, 583, 601, 676
MfeI CAATTG 1 cut(s) 384
MhlI GDGCHC 1 cut(s) 543
MluCI AATT 2 cut(s) 384, 625
MmeI TCCRAC 2 cut(s) 379, 480
MnlI CCTC 9 cut(s) 35, 65, 163, 241, 332, 332, 382, 544, 585
MseI TTAA 5 cut(s) 77, 102, 237, 428, 566
MspA1I CMGCKG 1 cut(s) 617
MspR9I CCNGG 1 cut(s) 622
MunI CAATTG 1 cut(s) 384
MvaI CCWGG 1 cut(s) 622
MwoI GCNNNNNNNGC 1 cut(s) 538
NcoI CCATGG 1 cut(s) 129
NdeII GATC 1 cut(s) 245
NlaIII CATG 2 cut(s) 50, 133
PciSI GCTCTTC 1 cut(s) 59
PfeI GAWTC 2 cut(s) 97, 125
PkrI GCNGC 3 cut(s) 370, 616, 619
PsiI TTATAA 1 cut(s) 585
Psp124BI GAGCTC 1 cut(s) 543
Psp6I CCWGG 1 cut(s) 620
PspGI CCWGG 1 cut(s) 620
PspPI GGNCC 1 cut(s) 352
PvuII CAGCTG 1 cut(s) 617
SacI GAGCTC 1 cut(s) 543
SapI GCTCTTC 1 cut(s) 59
SaqAI TTAA 5 cut(s) 77, 102, 237, 428, 566
SatI GCNGC 3 cut(s) 369, 615, 618
Sau3AI GATC 1 cut(s) 245
Sau96I GGNCC 1 cut(s) 352
ScrFI CCNGG 1 cut(s) 622
SduI GDGCHC 1 cut(s) 543
SfaNI GCATC 1 cut(s) 61
SinI GGWCC 1 cut(s) 352
SmlI CTYRAG 2 cut(s) 56, 212
SmoI CTYRAG 2 cut(s) 56, 212
Sse9I AATT 2 cut(s) 384, 625
SsiI CCGC 3 cut(s) 342, 647, 707
SspI AATATT 1 cut(s) 109
SspMI CTAG 2 cut(s) 476, 495
SstI GAGCTC 1 cut(s) 543
StyD4I CCNGG 1 cut(s) 620
StyI CCWWGG 2 cut(s) 129, 494
TaaI ACNGT 1 cut(s) 82
TaqI TCGA 3 cut(s) 244, 543, 600
TasI AATT 2 cut(s) 384, 625
TfiI GAWTC 2 cut(s) 97, 125
Tru1I TTAA 5 cut(s) 77, 102, 237, 428, 566
Tru9I TTAA 5 cut(s) 77, 102, 237, 428, 566
TscAI CASTG 2 cut(s) 87, 331
TseI GCWGC 3 cut(s) 368, 614, 617
TspDTI ATGAA 3 cut(s) 29, 158, 453
TspGWI ACGGA 1 cut(s) 80
TspRI CASTG 2 cut(s) 87, 331
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 1 cut(s) 625
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmaJI CCTAGG 1 cut(s) 494
XspI CTAG 2 cut(s) 476, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.