FvH4_6g47900

Cytosolic domain of 10TM putative phosphate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000893
Physical Location & Seq
Reverse (-)
36196819 .. 36202686
5868 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g47900.t2

Sequence Viewer

Length: 2151 bp
ATGATCCTCTCAGCGTTATTAACCTCGGTGGGAATCAATCTGGGTCTGTGTTTGCTTTTCTTCACTCTCTACTCTATTCTGAAGAAGCAGCCCATTAACGCCAAGGTCTACGCGCCGCGCTTCGTCGCTGCGAAGGAGAAAGAGAAAGAGTCACCATCAGAAACAGAAGCAAATGGGTTCAATTTCGAGAGATTGTTGCCCACTGCTGGCTGGGTGAGACGGGCATGGCAGCCTTCTGAAGATCAGTTCTTATCTGCCACCAGCTTGGATGCTGTGGTTTTCATGCGCATCTTCGTGTTCAGTTTGAGGGTGTTTGGGTTTGCTGCGGTTATTGGGGTGTTTGTTCTTCTGCCGATAAATTTCCTGGGGACCCAGCTGGAGGTGGACTTTTCGGATTTGACCAACAAGTCTTTGGACTCGTTTAGTATTTCGAACGTTGATGATGGCTCAAACTGGTTATGGGTTCATTTCTGTGCAGCATATATTTTCAGTGCATTTGTCTGCTATCTTCTTTACAATGAGTACAGCTATATCTCATCAAGAAGAATTGCTCACTTCTATTCATCCAAACCTCAGCCTAATCAGTTCACAGTATTGGTCCGTGGTATTCCTGTCCCGTCTGGGAGCAGCAGCAGTGAAATCATTGAAAACTTTTTTACAGAATATTACTCTTCTACTTACCTTTCACACACGATGGTTCGTCGAACAAGCAAACTCCAACGTCTAACTAAAGATGCAGATAAGGTATACAGAACGCTTGCCCGTATCAAGTCAGAAAAGGATCCACAGCAAAGATTCAAGCGTGATGGGCTTTTTGGACGGAAAGTTGATGCTTTGGATCACTATGGAAAGAAATTGGATGATTTAGAAGATACTGTTAGATTAGAGCAATCATCATTGGCAGGAAAGGAAGTTTCAGCCGCTTTTGTGTCATTCAAGTCCCGACTTGCTGCTGCAGTAGCTTTACACATTCAACAAGGAACAAATCCCACAGAGTGGGTCACAGAGAGCGCTCCTGAGCCCCAGGATGTTCATTGGCCCTTCTTTTCTTCATCTTTTATAAAAAGATGGATCTGCAAGCTGGTGGTGCTGGTTGCATATGCTGCTGTTACAATTCTATATCTCATTCCAGTTGTAATCGTGCAAGGCCTCGCTAATCTTTCAACTTTAGAAACCTGGTTTCCTTTTCTCACAAGCATACTGACTTTAACAGTTGTCAGTGAAGTCATAACTGGATATCTTCCAAGTCTTATTCTTCAGTTGTTCCTTAAACTTGTTCCACCCATCATGATAGTGCTGTCATCTATGGAAGGGTACATCTCCTTCAGTCAAATACAAAAAGGTGCATGCATGAAGATGTTGTTGTTTACTATATGGAATATTTTCTTTGCAAATGTACTATCAGGAACAGCTCTCTATCGATTCGATATCCTTCTTGAGCCCAAAAAGATTCCCGGGCTACTTGCTGATGCTGTTCCGGCACAGGCATCATTCTTCATTTCATATGTTGTGACATCTGGATGGACCAGTGTATCATCCGAACTCTTCCGCATGATTCCCTTGATTTTCAGTATTATAAAAAGACTATTTTCTGGAAAAGATGGTGATGAATTTGAGGTTCCTTCAATTAAATACCACAGCGAAATTCCTAGAATTCTATTTTTTGGAGTCCTTGGAATAACATACTTCTTGCTGGCTCCATTAATTCTACCTTTCCTTCTGGTTTACTGCTGTATGGGATACCTAATCTACCGCAACCAGCTATTGAATGTATATGCACCCAAGTATGACACTGGTGGAAAATTTTGGCCTATAGTGCACGGTTCAACAATATTTTCCTTGGTACTTATGCACATCATTGCGATTGGAATGTTTGGGATTAAAGATCTTGCCTTAGCTTCTAGTTTGATGGTTCCTCTTCCAATTCTCACTCTTCTTTTCAATGAGTACTGCCGCAAACGATTTTTACCGATATTCGAATCATATCCTGTTGAGTGTTTGGTAAAGAAAGATAAAGAAGACCAGAGTGATCCTTCAATGGATGCATTTTATGATAAATTAAGCACTGCATATGAGGATCCAGCTTTGATACCAAGACGTCAAAGAAGCACAGATGGCCACAACTCTCCACTTCTTCCCGCTGAAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

717

Amino Acids

80.89

Weight (kDa)

8.35

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 5 - 174 3.5e-40 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 195 - 347 5.9e-38 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 360 - 495 3.8e-29 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1061, 1577
AasI GACNNNNNNGTC 1 cut(s) 406
AatII GACGTC 1 cut(s) 2101
Acc16I TGCGCA 1 cut(s) 287
AccB7I CCANNNNNTGG 1 cut(s) 996
AccI GTMKAC 2 cut(s) 108, 747
AccII CGCG 2 cut(s) 113, 118
AciI CCGC 7 cut(s) 116, 326, 921, 1549, 1753, 1954, 2139
AclI AACGTT 1 cut(s) 435
AclWI GGATC 7 cut(s) 776, 789, 846, 1079, 2024, 2072, 2085
AcoI YGGCCR 1 cut(s) 2116
AcsI RAATTY 5 cut(s) 358, 1610, 1644, 1653, 1802
AcuI CTGAAG 4 cut(s) 101, 258, 1241, 1309
AcyI GRCGYC 1 cut(s) 2098
AdeI CACNNNGTG 1 cut(s) 996
AfaI GTAC 5 cut(s) 524, 1316, 1398, 1845, 1949
AfeI AGCGCT 1 cut(s) 1012
AfiI CCNNNNNNNGG 3 cut(s) 206, 379, 996
AjnI CCWGG 3 cut(s) 363, 1023, 1175
AluBI AGCT 9 cut(s) 264, 376, 528, 962, 1081, 1412, 1762, 1898, 2084
AluI AGCT 9 cut(s) 264, 376, 528, 962, 1081, 1412, 1762, 1898, 2084
Alw21I GWGCWC 1 cut(s) 1821
Alw26I GTCTC 1 cut(s) 211
Alw44I GTGCAC 1 cut(s) 1817
AlwI GGATC 7 cut(s) 776, 789, 846, 1079, 2024, 2072, 2085
Ama87I CYCGRG 1 cut(s) 1454
Aor51HI AGCGCT 1 cut(s) 1012
AoxI GGCC 4 cut(s) 1037, 1147, 1808, 2116
ApaLI GTGCAC 1 cut(s) 1817
ApoI RAATTY 5 cut(s) 358, 1610, 1644, 1653, 1802
AseI ATTAAT 1 cut(s) 1703
Asp700I GAANNNNTTC 1 cut(s) 1382
AspLEI GCGC 4 cut(s) 115, 120, 288, 1013
AspS9I GGNCC 4 cut(s) 369, 598, 1038, 1524
AsuC2I CCSGG 2 cut(s) 1455, 1456
AsuHPI GGTGA 3 cut(s) 144, 226, 1616
AsuII TTCGAA 2 cut(s) 431, 1977
AvaI CYCGRG 1 cut(s) 1454
AvaII GGWCC 3 cut(s) 369, 598, 1524
BaeGI GKGCMC 1 cut(s) 1821
BaeI ACNNNNGTAYC 2 cut(s) 1515, 1548
BalI TGGCCA 1 cut(s) 2118
BamHI GGATCC 2 cut(s) 781, 2077
BanII GRGCYC 2 cut(s) 1023, 1443
BarI GAAGNNNNNNTAC 2 cut(s) 1307, 1339
BbsI GAAGAC 1 cut(s) 2025
Bbv12I GWGCWC 1 cut(s) 1821
BbvCI CCTCAGC 1 cut(s) 573
BciT130I CCWGG 3 cut(s) 365, 1025, 1177
BciVI GTATCC 1 cut(s) 1733
BcnI CCSGG 2 cut(s) 1455, 1456
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 2 cut(s) 1650, 1902
BfmI CTRYAG 2 cut(s) 954, 1812
BfoI RGCGCY 1 cut(s) 1014
BfuI GTATCC 1 cut(s) 1733
BglII AGATCT 1 cut(s) 1885
BmcAI AGTACT 1 cut(s) 1949
Bme1390I CCNGG 5 cut(s) 365, 1025, 1177, 1455, 1456
Bme18I GGWCC 3 cut(s) 369, 598, 1524
BmeT110I CYCGRG 1 cut(s) 1454
BmgT120I GGNCC 4 cut(s) 369, 598, 1038, 1524
BmiI GGNNCC 7 cut(s) 370, 371, 783, 1620, 1698, 1914, 2079
BmrFI CCNGG 5 cut(s) 365, 1025, 1177, 1455, 1456
BmsI GCATC 7 cut(s) 259, 297, 724, 820, 1459, 1496, 2032
BpiI GAAGAC 1 cut(s) 2025
BpmI CTGGAG 1 cut(s) 398
Bpu10I CCTNAGC 3 cut(s) 573, 1017, 1894
Bpu14I TTCGAA 2 cut(s) 431, 1977
BpuEI CTTGAG 1 cut(s) 1457
BpuMI CCSGG 2 cut(s) 1455, 1456
Bsa29I ATCGAT 1 cut(s) 1420
BsaHI GRCGYC 1 cut(s) 2098
BsaJI CCNNGG 8 cut(s) 24, 102, 364, 601, 1023, 1454, 1672, 1839
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 379, 996
Bse1I ACTGG 5 cut(s) 458, 1130, 1237, 1527, 1798
Bse3DI GCAATG 1 cut(s) 1857
BseBI CCWGG 3 cut(s) 365, 1025, 1177
BseCI ATCGAT 1 cut(s) 1420
BseDI CCNNGG 8 cut(s) 24, 102, 364, 601, 1023, 1454, 1672, 1839
BseGI GGATG 7 cut(s) 274, 563, 865, 1033, 1526, 1535, 2047
BseLI CCNNNNNNNGG 3 cut(s) 206, 379, 996
BseMI GCAATG 1 cut(s) 1857
BseMII CTCAG 3 cut(s) 24, 587, 1008
BseNI ACTGG 5 cut(s) 458, 1130, 1237, 1527, 1798
BseSI GKGCMC 1 cut(s) 1821
BseYI CCCAGC 2 cut(s) 210, 372
BsgI GTGCAG 1 cut(s) 495
Bsh1236I CGCG 2 cut(s) 113, 118
BshFI GGCC 4 cut(s) 1039, 1149, 1810, 2118
BshVI ATCGAT 1 cut(s) 1420
BsiHKAI GWGCWC 1 cut(s) 1821
BsiHKCI CYCGRG 1 cut(s) 1454
BsiSI CCGG 2 cut(s) 1455, 1478
BslFI GGGAC 3 cut(s) 382, 599, 925
BslI CCNNNNNNNGG 3 cut(s) 206, 379, 996
BsmAI GTCTC 1 cut(s) 211
BsmBI CGTCTC 1 cut(s) 211
BsmFI GGGAC 3 cut(s) 382, 599, 925
BsnI GGCC 4 cut(s) 1039, 1149, 1810, 2118
BsoBI CYCGRG 1 cut(s) 1454
Bsp119I TTCGAA 2 cut(s) 431, 1977
Bsp1286I GDGCHC 3 cut(s) 1023, 1443, 1821
Bsp143I GATC 8 cut(s) 3, 241, 781, 838, 1071, 1885, 2029, 2077
BspACI CCGC 7 cut(s) 116, 326, 921, 1549, 1753, 1954, 2139
BspANI GGCC 4 cut(s) 1039, 1149, 1810, 2118
BspCNI CTCAG 3 cut(s) 23, 586, 1009
BspDI ATCGAT 1 cut(s) 1420
BspFNI CGCG 2 cut(s) 113, 118
BspHI TCATGA 1 cut(s) 1287
BspLI GGNNCC 7 cut(s) 370, 371, 783, 1620, 1698, 1914, 2079
BspMAI CTGCAG 1 cut(s) 958
BspPI GGATC 7 cut(s) 776, 789, 846, 1079, 2024, 2072, 2085
BspT104I TTCGAA 2 cut(s) 431, 1977
BsrDI GCAATG 1 cut(s) 1857
BsrI ACTGG 5 cut(s) 458, 1130, 1237, 1527, 1798
BssECI CCNNGG 8 cut(s) 24, 102, 364, 601, 1023, 1454, 1672, 1839
BssMI GATC 8 cut(s) 3, 241, 781, 838, 1071, 1885, 2029, 2077
BssNAI GTATAC 1 cut(s) 748
BssNI GRCGYC 1 cut(s) 2098
BssT1I CCWWGG 3 cut(s) 102, 1672, 1839
Bst1107I GTATAC 1 cut(s) 748
Bst2UI CCWGG 3 cut(s) 365, 1025, 1177
Bst4CI ACNGT 4 cut(s) 592, 877, 1213, 1823
Bst6I CTCTTC 4 cut(s) 676, 1550, 1923, 1938
BstACI GRCGYC 1 cut(s) 2098
BstAPI GCANNNNNTGC 1 cut(s) 1103
BstBI TTCGAA 2 cut(s) 431, 1977
BstC8I GCNNGC 5 cut(s) 208, 759, 1079, 1348, 1695
BstDEI CTNAG 4 cut(s) 10, 573, 1017, 1894
BstDSI CCRYGG 1 cut(s) 601
BstF5I GGATG 7 cut(s) 274, 563, 865, 1033, 1526, 1535, 2047
BstFNI CGCG 2 cut(s) 113, 118
BstH2I RGCGCY 1 cut(s) 1014
BstHHI GCGC 4 cut(s) 115, 120, 288, 1013
BstKTI GATC 8 cut(s) 6, 244, 784, 841, 1074, 1888, 2032, 2080
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 8 cut(s) 3, 241, 781, 838, 1071, 1885, 2029, 2077
BstMWI GCNNNNNNNGC 7 cut(s) 808, 959, 1087, 1103, 1478, 1816, 2115
BstNI CCWGG 3 cut(s) 365, 1025, 1177
BstNSI RCATGY 1 cut(s) 1350
BstSCI CCNGG 5 cut(s) 363, 1023, 1175, 1453, 1454
BstSFI CTRYAG 2 cut(s) 954, 1812
BstSLI GKGCMC 1 cut(s) 1821
BstUI CGCG 2 cut(s) 113, 118
BstV2I GAAGAC 1 cut(s) 2025
BstX2I RGATCY 4 cut(s) 781, 1071, 1885, 2077
BstXI CCANNNNNNTGG 1 cut(s) 265
BstYI RGATCY 4 cut(s) 781, 1071, 1885, 2077
BstZ17I GTATAC 1 cut(s) 748
Bsu15I ATCGAT 1 cut(s) 1420
BsuI GTATCC 1 cut(s) 1733
BsuRI GGCC 4 cut(s) 1039, 1149, 1810, 2118
BsuTUI ATCGAT 1 cut(s) 1420
BtgI CCRYGG 1 cut(s) 601
BtsCI GGATG 7 cut(s) 274, 563, 865, 1033, 1526, 1535, 2047
BtsI GCAGTG 3 cut(s) 201, 640, 2064
BtsIMutI CAGTG 7 cut(s) 201, 496, 640, 1225, 1534, 1791, 2064
Cac8I GCNNGC 5 cut(s) 208, 759, 1079, 1348, 1695
CciI TCATGA 1 cut(s) 1287
CfoI GCGC 4 cut(s) 115, 120, 288, 1013
Cfr13I GGNCC 4 cut(s) 369, 598, 1038, 1524
Cfr9I CCCGGG 1 cut(s) 1454
ClaI ATCGAT 1 cut(s) 1420
CsiI ACCWGGT 1 cut(s) 1175
Csp6I GTAC 5 cut(s) 523, 1315, 1397, 1844, 1948
CviAII CATG 6 cut(s) 225, 283, 1288, 1347, 1351, 1552
CviQI GTAC 5 cut(s) 523, 1315, 1397, 1844, 1948
DdeI CTNAG 4 cut(s) 10, 573, 1017, 1894
DpnI GATC 8 cut(s) 5, 243, 783, 840, 1073, 1887, 2031, 2079
DpnII GATC 8 cut(s) 3, 241, 781, 838, 1071, 1885, 2029, 2077
DraIII CACNNNGTG 1 cut(s) 996
DrdI GACNNNNNNGTC 1 cut(s) 406
DseDI GACNNNNNNGTC 1 cut(s) 406
EaeI YGGCCR 1 cut(s) 2116
Eam1104I CTCTTC 4 cut(s) 676, 1550, 1923, 1938
EarI CTCTTC 4 cut(s) 676, 1550, 1923, 1938
Eco130I CCWWGG 3 cut(s) 102, 1672, 1839
Eco147I AGGCCT 1 cut(s) 1149
Eco24I GRGCYC 2 cut(s) 1023, 1443
Eco32I GATATC 2 cut(s) 1238, 1429
Eco47I GGWCC 3 cut(s) 369, 598, 1524
Eco47III AGCGCT 1 cut(s) 1012
Eco57I CTGAAG 4 cut(s) 101, 258, 1241, 1309
Eco88I CYCGRG 1 cut(s) 1454
EcoO109I RGGNCCY 1 cut(s) 369
EcoRI GAATTC 1 cut(s) 1653
EcoRII CCWGG 3 cut(s) 363, 1023, 1175
EcoRV GATATC 2 cut(s) 1238, 1429
EcoT14I CCWWGG 3 cut(s) 102, 1672, 1839
EcoT22I ATGCAT 2 cut(s) 1352, 2047
EcoT38I GRGCYC 2 cut(s) 1023, 1443
ErhI CCWWGG 3 cut(s) 102, 1672, 1839
Esp3I CGTCTC 1 cut(s) 211
FaeI CATG 6 cut(s) 228, 286, 1291, 1350, 1354, 1555
FaqI GGGAC 3 cut(s) 382, 599, 925
FatI CATG 6 cut(s) 224, 282, 1287, 1346, 1350, 1551
FauI CCCGC 1 cut(s) 2146
FauNDI CATATG 3 cut(s) 1099, 1504, 2071
FblI GTMKAC 2 cut(s) 108, 747
FokI GGATG 7 cut(s) 281, 550, 872, 1040, 1522, 1533, 2054
FriOI GRGCYC 2 cut(s) 1023, 1443
FspAI RTGCGCAY 1 cut(s) 287
FspBI CTAG 2 cut(s) 1650, 1902
FspI TGCGCA 1 cut(s) 287
GlaI GCGC 4 cut(s) 114, 119, 287, 1012
GsaI CCCAGC 2 cut(s) 214, 376
GsuI CTGGAG 1 cut(s) 398
HaeII RGCGCY 1 cut(s) 1014
HaeIII GGCC 4 cut(s) 1039, 1149, 1810, 2118
HapII CCGG 2 cut(s) 1455, 1478
HhaI GCGC 4 cut(s) 115, 120, 288, 1013
Hin1I GRCGYC 1 cut(s) 2098
Hin1II CATG 6 cut(s) 228, 286, 1291, 1350, 1354, 1555
Hin6I GCGC 4 cut(s) 113, 118, 286, 1011
HinP1I GCGC 4 cut(s) 113, 118, 286, 1011
HinfI GANTC 9 cut(s) 33, 149, 416, 795, 1422, 1450, 1555, 1668, 1979
HpaII CCGG 2 cut(s) 1455, 1478
HphI GGTGA 3 cut(s) 144, 226, 1616
Hpy166II GTNNAC 7 cut(s) 109, 385, 588, 748, 1368, 1726, 1819
Hpy188I TCNGA 7 cut(s) 81, 160, 238, 394, 775, 1540, 2150
Hpy188III TCNNGA 9 cut(s) 187, 540, 942, 1016, 1288, 1404, 1436, 1518, 1593
Hpy8I GTNNAC 7 cut(s) 109, 385, 588, 748, 1368, 1726, 1819
Hpy99I CGWCG 2 cut(s) 128, 705
HpyAV CCTTC 9 cut(s) 127, 243, 1051, 1304, 1333, 1442, 1632, 1727, 2043
HpyCH4III ACNGT 4 cut(s) 592, 877, 1213, 1823
HpyCH4IV ACGT 3 cut(s) 435, 721, 2098
HpyF10VI GCNNNNNNNGC 7 cut(s) 808, 959, 1087, 1103, 1478, 1816, 2115
HpyF3I CTNAG 4 cut(s) 10, 573, 1017, 1894
HpySE526I ACGT 3 cut(s) 435, 721, 2098
Hsp92I GRCGYC 1 cut(s) 2098
Hsp92II CATG 6 cut(s) 228, 286, 1291, 1350, 1354, 1555
HspAI GCGC 4 cut(s) 113, 118, 286, 1011
KflI GGGWCCC 1 cut(s) 369
Kzo9I GATC 8 cut(s) 3, 241, 781, 838, 1071, 1885, 2029, 2077
LmnI GCTCC 3 cut(s) 624, 1018, 1702
LweI GCATC 7 cut(s) 259, 297, 724, 820, 1459, 1496, 2032
MabI ACCWGGT 1 cut(s) 1175
MaeI CTAG 2 cut(s) 1650, 1902
MaeII ACGT 3 cut(s) 435, 721, 2098
MaeIII GTNAC 4 cut(s) 150, 1000, 1108, 1510
MalI GATC 8 cut(s) 5, 243, 783, 840, 1073, 1887, 2031, 2079
MboI GATC 8 cut(s) 3, 241, 781, 838, 1071, 1885, 2029, 2077
MflI RGATCY 4 cut(s) 781, 1071, 1885, 2077
MhlI GDGCHC 3 cut(s) 1023, 1443, 1821
MlsI TGGCCA 1 cut(s) 2118
MluNI TGGCCA 1 cut(s) 2118
MlyI GAGTC 3 cut(s) 158, 410, 1677
MmeI TCCRAC 1 cut(s) 742
MnlI CCTC 9 cut(s) 17, 34, 300, 373, 582, 1160, 1609, 1926, 2068
Mox20I TGGCCA 1 cut(s) 2118
Mph1103I ATGCAT 2 cut(s) 1352, 2047
MroXI GAANNNNTTC 1 cut(s) 1382
MscI TGGCCA 1 cut(s) 2118
MseI TTAA 8 cut(s) 20, 96, 1208, 1269, 1629, 1703, 1881, 2060
MslI CAYNNNNRTG 5 cut(s) 293, 471, 1292, 1355, 1519
Msp20I TGGCCA 1 cut(s) 2118
MspA1I CMGCKG 2 cut(s) 376, 2141
MspI CCGG 2 cut(s) 1455, 1478
MspR9I CCNGG 5 cut(s) 365, 1025, 1177, 1455, 1456
MteI GCGCNGCGC 1 cut(s) 116
MvaI CCWGG 3 cut(s) 365, 1025, 1177
MvnI CGCG 2 cut(s) 113, 118
MwoI GCNNNNNNNGC 7 cut(s) 808, 959, 1087, 1103, 1478, 1816, 2115
NciI CCSGG 2 cut(s) 1455, 1456
NdeI CATATG 3 cut(s) 1099, 1504, 2071
NdeII GATC 8 cut(s) 3, 241, 781, 838, 1071, 1885, 2029, 2077
NlaIII CATG 6 cut(s) 228, 286, 1291, 1350, 1354, 1555
NlaIV GGNNCC 7 cut(s) 370, 371, 783, 1620, 1698, 1914, 2079
NmuCI GTSAC 3 cut(s) 150, 1000, 1510
NsbI TGCGCA 1 cut(s) 287
NsiI ATGCAT 2 cut(s) 1352, 2047
NspI RCATGY 1 cut(s) 1350
NspV TTCGAA 2 cut(s) 431, 1977
PaeI GCATGC 1 cut(s) 1350
PagI TCATGA 1 cut(s) 1287
PceI AGGCCT 1 cut(s) 1149
PdmI GAANNNNTTC 1 cut(s) 1382
PfeI GAWTC 6 cut(s) 33, 795, 1422, 1450, 1555, 1979
PflMI CCANNNNNTGG 1 cut(s) 996
PleI GAGTC 3 cut(s) 157, 410, 1676
PpsI GAGTC 3 cut(s) 157, 410, 1676
PpuMI RGGWCCY 1 cut(s) 369
PshBI ATTAAT 1 cut(s) 1703
PsiI TTATAA 2 cut(s) 1061, 1577
Psp1406I AACGTT 1 cut(s) 435
Psp5II RGGWCCY 1 cut(s) 369
Psp6I CCWGG 3 cut(s) 363, 1023, 1175
PspFI CCCAGC 2 cut(s) 210, 372
PspGI CCWGG 3 cut(s) 363, 1023, 1175
PspN4I GGNNCC 7 cut(s) 370, 371, 783, 1620, 1698, 1914, 2079
PspPI GGNCC 4 cut(s) 369, 598, 1038, 1524
PspPPI RGGWCCY 1 cut(s) 369
PstI CTGCAG 1 cut(s) 958
PsuI RGATCY 4 cut(s) 781, 1071, 1885, 2077
PvuII CAGCTG 1 cut(s) 376
RsaI GTAC 5 cut(s) 524, 1316, 1398, 1845, 1949
RsaNI GTAC 5 cut(s) 523, 1315, 1397, 1844, 1948
RseI CAYNNNNRTG 5 cut(s) 293, 471, 1292, 1355, 1519
SaqAI TTAA 8 cut(s) 20, 96, 1208, 1269, 1629, 1703, 1881, 2060
Sau3AI GATC 8 cut(s) 3, 241, 781, 838, 1071, 1885, 2029, 2077
Sau96I GGNCC 4 cut(s) 369, 598, 1038, 1524
ScaI AGTACT 1 cut(s) 1949
SchI GAGTC 3 cut(s) 158, 410, 1677
ScrFI CCNGG 5 cut(s) 365, 1025, 1177, 1455, 1456
SduI GDGCHC 3 cut(s) 1023, 1443, 1821
SexAI ACCWGGT 1 cut(s) 1175
SfaNI GCATC 7 cut(s) 259, 297, 724, 820, 1459, 1496, 2032
SfcI CTRYAG 2 cut(s) 954, 1812
SfuI TTCGAA 2 cut(s) 431, 1977
SinI GGWCC 3 cut(s) 369, 598, 1524
SmaI CCCGGG 1 cut(s) 1456
SmiMI CAYNNNNRTG 5 cut(s) 293, 471, 1292, 1355, 1519
SmlI CTYRAG 1 cut(s) 1436
SmoI CTYRAG 1 cut(s) 1436
SphI GCATGC 1 cut(s) 1350
SseBI AGGCCT 1 cut(s) 1149
SsiI CCGC 7 cut(s) 116, 326, 921, 1549, 1753, 1954, 2139
SspI AATATT 3 cut(s) 665, 1381, 1833
SspMI CTAG 2 cut(s) 1650, 1902
StuI AGGCCT 1 cut(s) 1149
StyD4I CCNGG 5 cut(s) 363, 1023, 1175, 1453, 1454
StyI CCWWGG 3 cut(s) 102, 1672, 1839
TaaI ACNGT 4 cut(s) 592, 877, 1213, 1823
TaiI ACGT 3 cut(s) 438, 724, 2101
TaqI TCGA 6 cut(s) 186, 431, 703, 1420, 1425, 1977
TatI WGTACW 3 cut(s) 522, 1396, 1947
TauI GCSGC 3 cut(s) 118, 923, 1956
TfiI GAWTC 6 cut(s) 33, 795, 1422, 1450, 1555, 1979
Tru1I TTAA 8 cut(s) 20, 96, 1208, 1269, 1629, 1703, 1881, 2060
Tru9I TTAA 8 cut(s) 20, 96, 1208, 1269, 1629, 1703, 1881, 2060
TscAI CASTG 7 cut(s) 208, 496, 640, 1225, 1534, 1798, 2071
TseFI GTSAC 3 cut(s) 150, 1000, 1510
Tsp45I GTSAC 3 cut(s) 150, 1000, 1510
TspDTI ATGAA 9 cut(s) 271, 455, 552, 1022, 1041, 1367, 1486, 1491, 1623
TspGWI ACGGA 2 cut(s) 590, 835
TspMI CCCGGG 1 cut(s) 1454
TspRI CASTG 7 cut(s) 208, 496, 640, 1225, 1534, 1798, 2071
Van91I CCANNNNNTGG 1 cut(s) 996
VneI GTGCAC 1 cut(s) 1817
VpaK11BI GGWCC 3 cut(s) 369, 598, 1524
VspI ATTAAT 1 cut(s) 1703
XapI RAATTY 5 cut(s) 358, 1610, 1644, 1653, 1802
XceI RCATGY 1 cut(s) 1350
XcmI CCANNNNNNNNNTGG 1 cut(s) 409
XmaI CCCGGG 1 cut(s) 1454
XmiI GTMKAC 2 cut(s) 108, 747
XmnI GAANNNNTTC 1 cut(s) 1382
XspI CTAG 2 cut(s) 1650, 1902
ZraI GACGTC 1 cut(s) 2099
ZrmI AGTACT 1 cut(s) 1949
Zsp2I ATGCAT 2 cut(s) 1352, 2047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.