pycom111g04680

Cytosolic domain of 10TM putative phosphate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0000893
Physical Location & Seq
Forward (+)
2839392 .. 2841869
2478 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g04680.4

Sequence Viewer

Length: 1161 bp
ATGGGTTACCGAGAGGGCCCCGGAGCCCCAGGATGTTCACTGGCCCTTCTTTTCTGCATCTTTTATAAAAAGATGGATCGATCCAAGCTGGTGGTGGTGGTTGCATATACTGCTCTTACAATTCTGTTCCTCATTCCAGTTGTAATTGTGCAAGGCCTTACTCATCTTGAGCAGTTGGAAACCTGGTTTCCGTTTCTGAAAAGCGTACTGGACCTAACAGTTGTCAGTGAAGTCATAACAGGATATCTTCCAAGTCTTATTCTCCAATTGTTCCTTTCTTTTGTGCCACCCATCATGATAATGTTGTCTTCCATGGAAGGATACATCTCTTTCAGTCAGATACAAAAAAGTGCATGCGGCAAGATGTTGTGGTTCACAATATGGAATATATTCTTGGCAAATACACTTTCAGGAACAGTTCTCTATCGGTTCAATATCTTTCTTGAGCCCAAAAAGATTCCGGGGATACTTGCTGATGCTGTTCCAGCACAGGCATCATTCTTCATTGCATATGTTGTGACATCTGGATGGACCAGTCTTCTAGCCTCAGAACTCTTCCGCGTGAGTCCCCTGATTTGGAGTATCATAAAAAGACCATTTTCAGGAAAAGATGATGAATTTGAGGTTCCTTCAATTCCATACCATAGTGAAATTCCTCGAGTTCTCTTTTTTGAACTCCTCGGAATAACATACTTCTTCCTAGCTCCACTAATTCTCCCGTTCCTCTTGGTTTACTGTTGTCTGGGATACATAATCTTCCGTAACCAGTTTCTGAATGTATATGCACCCAAGTATGAAACCGACGGAAAATTTTGGCCAACTGTGCACAATTCAACAATTTTTGCCTTGGTGCTGATGCACATCATTGCAATTGGAATGTTTGGGCTCAAAAAGGTTCCCTTAGCTTCCAGTCTGACCATTCCTCTTCCGATTCTCACGCTTCTTTTCAATGAGTACTGCCGCAAACGATTCCTTCCAATATTCAAAGCCTATCCGGCTGAGTGTTTGATTAAGAAAGATAGAGAAGACGAGAACGATCCTAGAATCTCTGCATTTTATGAAAAGTTAAGCACTGCTTACAGGGATCCAGCTCTGATGCCAATGCATCATCCGCGAAGCACTGATGGACACAGCACTCCCCTTCTTCAAGCTGGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

387

Amino Acids

43.76

Weight (kDa)

8.55

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 66
AccII CGCG 2 cut(s) 561, 1114
AciI CCGC 4 cut(s) 357, 559, 961, 1112
AclWI GGATC 5 cut(s) 75, 84, 1031, 1079, 1092
AcoI YGGCCR 1 cut(s) 815
AcsI RAATTY 3 cut(s) 617, 651, 809
AfaI GTAC 2 cut(s) 207, 956
AfiI CCNNNNNNNGG 2 cut(s) 576, 602
AgsI TTSAA 7 cut(s) 433, 633, 674, 834, 949, 985, 1148
AjnI CCWGG 2 cut(s) 28, 182
AjuI GAANNNNNNNTTGG 2 cut(s) 377, 409
AluBI AGCT 5 cut(s) 88, 704, 905, 1091, 1151
AluI AGCT 5 cut(s) 88, 704, 905, 1091, 1151
Alw21I GWGCWC 1 cut(s) 828
Alw44I GTGCAC 1 cut(s) 824
AlwI GGATC 5 cut(s) 75, 84, 1031, 1079, 1092
AlwNI CAGNNNCTG 1 cut(s) 772
Ama87I CYCGRG 1 cut(s) 657
AoxI GGCC 4 cut(s) 16, 42, 154, 815
ApaI GGGCCC 1 cut(s) 20
ApaLI GTGCAC 1 cut(s) 824
ApoI RAATTY 3 cut(s) 617, 651, 809
Asp700I GAANNNNTTC 1 cut(s) 389
AspS9I GGNCC 5 cut(s) 16, 17, 43, 211, 531
AsuC2I CCSGG 2 cut(s) 21, 462
AvaI CYCGRG 1 cut(s) 657
AvaII GGWCC 2 cut(s) 211, 531
BaeGI GKGCMC 2 cut(s) 20, 828
BalI TGGCCA 1 cut(s) 817
BamHI GGATCC 1 cut(s) 1084
BanII GRGCYC 4 cut(s) 20, 28, 450, 888
BbsI GAAGAC 3 cut(s) 300, 530, 1032
Bbv12I GWGCWC 1 cut(s) 828
BccI CCATC 4 cut(s) 67, 299, 522, 1118
BciT130I CCWGG 2 cut(s) 30, 184
BciVI GTATCC 3 cut(s) 314, 459, 740
BcnI CCSGG 2 cut(s) 21, 462
BfaI CTAG 3 cut(s) 542, 701, 1041
BfuI GTATCC 3 cut(s) 314, 459, 740
BglI GCCNNNNNGGC 1 cut(s) 995
BisI GCNGC 2 cut(s) 358, 961
BlsI GCNGC 2 cut(s) 359, 962
BmcAI AGTACT 1 cut(s) 956
Bme1390I CCNGG 4 cut(s) 21, 30, 184, 462
Bme18I GGWCC 2 cut(s) 211, 531
BmeT110I CYCGRG 1 cut(s) 657
BmgT120I GGNCC 5 cut(s) 16, 17, 43, 211, 531
BmiI GGNNCC 6 cut(s) 18, 19, 25, 627, 897, 1086
BmrFI CCNGG 4 cut(s) 21, 30, 184, 462
BmsI GCATC 6 cut(s) 66, 466, 503, 846, 1086, 1114
BpiI GAAGAC 3 cut(s) 300, 530, 1032
Bpu10I CCTNAGC 1 cut(s) 901
BpuEI CTTGAG 2 cut(s) 188, 464
BpuMI CCSGG 2 cut(s) 21, 462
Bsa29I ATCGAT 1 cut(s) 79
BsaBI GATNNNNATC 1 cut(s) 860
BsaJI CCNNGG 6 cut(s) 19, 28, 312, 461, 679, 846
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 2 cut(s) 576, 602
Bse1I ACTGG 6 cut(s) 45, 137, 213, 534, 766, 909
Bse3DI GCAATG 2 cut(s) 504, 864
Bse8I GATNNNNATC 1 cut(s) 860
BseBI CCWGG 2 cut(s) 30, 184
BseCI ATCGAT 1 cut(s) 79
BseDI CCNNGG 6 cut(s) 19, 28, 312, 461, 679, 846
BseGI GGATG 3 cut(s) 38, 533, 1108
BseJI GATNNNNATC 1 cut(s) 860
BseLI CCNNNNNNNGG 2 cut(s) 576, 602
BseMI GCAATG 2 cut(s) 504, 864
BseMII CTCAG 2 cut(s) 561, 990
BseNI ACTGG 6 cut(s) 45, 137, 213, 534, 766, 909
BseRI GAGGAG 1 cut(s) 668
BseSI GKGCMC 2 cut(s) 20, 828
Bsh1236I CGCG 2 cut(s) 561, 1114
BshFI GGCC 4 cut(s) 18, 44, 156, 817
BshVI ATCGAT 1 cut(s) 79
BsiHKAI GWGCWC 1 cut(s) 828
BsiHKCI CYCGRG 1 cut(s) 657
BsiSI CCGG 3 cut(s) 21, 461, 995
BslFI GGGAC 1 cut(s) 552
BslI CCNNNNNNNGG 2 cut(s) 576, 602
BsmFI GGGAC 1 cut(s) 552
BsnI GGCC 4 cut(s) 18, 44, 156, 817
BsoBI CYCGRG 1 cut(s) 657
Bsp120I GGGCCC 1 cut(s) 16
Bsp1286I GDGCHC 5 cut(s) 20, 28, 450, 828, 888
Bsp143I GATC 4 cut(s) 76, 80, 1036, 1084
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 4 cut(s) 357, 559, 961, 1112
BspANI GGCC 4 cut(s) 18, 44, 156, 817
BspCNI CTCAG 2 cut(s) 560, 991
BspDI ATCGAT 1 cut(s) 79
BspFNI CGCG 2 cut(s) 561, 1114
BspHI TCATGA 1 cut(s) 294
BspLI GGNNCC 6 cut(s) 18, 19, 25, 627, 897, 1086
BspPI GGATC 5 cut(s) 75, 84, 1031, 1079, 1092
BsrDI GCAATG 2 cut(s) 504, 864
BsrI ACTGG 6 cut(s) 45, 137, 213, 534, 766, 909
BssECI CCNNGG 6 cut(s) 19, 28, 312, 461, 679, 846
BssMI GATC 4 cut(s) 76, 80, 1036, 1084
BssT1I CCWWGG 2 cut(s) 312, 846
Bst2UI CCWGG 2 cut(s) 30, 184
Bst4CI ACNGT 4 cut(s) 220, 418, 737, 823
Bst6I CTCTTC 2 cut(s) 560, 930
BstAPI GCANNNNNTGC 1 cut(s) 110
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 3 cut(s) 547, 901, 999
BstDSI CCRYGG 1 cut(s) 312
BstEII GGTNACC 1 cut(s) 5
BstF5I GGATG 3 cut(s) 38, 533, 1108
BstFNI CGCG 2 cut(s) 561, 1114
BstKTI GATC 4 cut(s) 79, 83, 1039, 1087
BstMBI GATC 4 cut(s) 76, 80, 1036, 1084
BstMWI GCNNNNNNNGC 5 cut(s) 110, 485, 823, 995, 1111
BstNI CCWGG 2 cut(s) 30, 184
BstNSI RCATGY 1 cut(s) 357
BstPI GGTNACC 1 cut(s) 5
BstSCI CCNGG 4 cut(s) 19, 28, 182, 460
BstSLI GKGCMC 2 cut(s) 20, 828
BstUI CGCG 2 cut(s) 561, 1114
BstV2I GAAGAC 3 cut(s) 300, 530, 1032
BstX2I RGATCY 1 cut(s) 1084
BstXI CCANNNNNNTGG 1 cut(s) 91
BstYI RGATCY 1 cut(s) 1084
Bsu15I ATCGAT 1 cut(s) 79
BsuI GTATCC 3 cut(s) 314, 459, 740
BsuRI GGCC 4 cut(s) 18, 44, 156, 817
BsuTUI ATCGAT 1 cut(s) 79
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 3 cut(s) 38, 533, 1108
BtsI GCAGTG 1 cut(s) 1071
BtsIMutI CAGTG 4 cut(s) 38, 232, 1071, 1119
Cac8I GCNNGC 1 cut(s) 355
CaiI CAGNNNCTG 1 cut(s) 772
CciI TCATGA 1 cut(s) 294
Cfr13I GGNCC 5 cut(s) 16, 17, 43, 211, 531
ClaI ATCGAT 1 cut(s) 79
CsiI ACCWGGT 1 cut(s) 182
Csp6I GTAC 2 cut(s) 206, 955
CviAII CATG 3 cut(s) 295, 313, 354
CviQI GTAC 2 cut(s) 206, 955
DdeI CTNAG 3 cut(s) 547, 901, 999
DpnI GATC 4 cut(s) 78, 82, 1038, 1086
DpnII GATC 4 cut(s) 76, 80, 1036, 1084
EaeI YGGCCR 1 cut(s) 815
Eam1104I CTCTTC 2 cut(s) 560, 930
EarI CTCTTC 2 cut(s) 560, 930
Eco130I CCWWGG 2 cut(s) 312, 846
Eco147I AGGCCT 1 cut(s) 156
Eco24I GRGCYC 4 cut(s) 20, 28, 450, 888
Eco32I GATATC 1 cut(s) 245
Eco47I GGWCC 2 cut(s) 211, 531
Eco88I CYCGRG 1 cut(s) 657
Eco91I GGTNACC 1 cut(s) 5
EcoO109I RGGNCCY 2 cut(s) 16, 17
EcoO65I GGTNACC 1 cut(s) 5
EcoRII CCWGG 2 cut(s) 28, 182
EcoRV GATATC 1 cut(s) 245
EcoT14I CCWWGG 2 cut(s) 312, 846
EcoT22I ATGCAT 1 cut(s) 1107
EcoT38I GRGCYC 4 cut(s) 20, 28, 450, 888
ErhI CCWWGG 2 cut(s) 312, 846
FaeI CATG 3 cut(s) 298, 316, 357
FalI AAGNNNNNCTT 4 cut(s) 884, 916, 1060, 1092
FaqI GGGAC 1 cut(s) 552
FatI CATG 3 cut(s) 294, 312, 353
FauNDI CATATG 1 cut(s) 511
Fnu4HI GCNGC 2 cut(s) 358, 961
FokI GGATG 3 cut(s) 45, 540, 1095
FriOI GRGCYC 4 cut(s) 20, 28, 450, 888
Fsp4HI GCNGC 2 cut(s) 358, 961
FspBI CTAG 3 cut(s) 542, 701, 1041
GluI GCNGC 2 cut(s) 358, 961
HaeIII GGCC 4 cut(s) 18, 44, 156, 817
HapII CCGG 3 cut(s) 21, 461, 995
Hin1II CATG 3 cut(s) 298, 316, 357
HinfI GANTC 6 cut(s) 457, 565, 931, 969, 1044, 1155
HpaII CCGG 3 cut(s) 21, 461, 995
Hpy166II GTNNAC 4 cut(s) 38, 375, 733, 826
Hpy188I TCNGA 9 cut(s) 198, 339, 550, 683, 774, 915, 930, 1095, 1160
Hpy188III TCNNGA 6 cut(s) 167, 295, 411, 443, 525, 603
Hpy8I GTNNAC 4 cut(s) 38, 375, 733, 826
Hpy99I CGWCG 1 cut(s) 806
HpyAV CCTTC 5 cut(s) 56, 311, 639, 983, 1151
HpyCH4III ACNGT 4 cut(s) 220, 418, 737, 823
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 485, 823, 995, 1111
HpyF3I CTNAG 3 cut(s) 547, 901, 999
Hsp92II CATG 3 cut(s) 298, 316, 357
Kzo9I GATC 4 cut(s) 76, 80, 1036, 1084
LmnI GCTCC 2 cut(s) 23, 709
LweI GCATC 6 cut(s) 66, 466, 503, 846, 1086, 1114
MabI ACCWGGT 1 cut(s) 182
MaeI CTAG 3 cut(s) 542, 701, 1041
MaeIII GTNAC 3 cut(s) 5, 517, 761
MalI GATC 4 cut(s) 78, 82, 1038, 1086
MboI GATC 4 cut(s) 76, 80, 1036, 1084
MfeI CAATTG 2 cut(s) 266, 870
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 5 cut(s) 20, 28, 450, 828, 888
MlsI TGGCCA 1 cut(s) 817
MluNI TGGCCA 1 cut(s) 817
MlyI GAGTC 1 cut(s) 574
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 8 cut(s) 7, 140, 556, 616, 666, 689, 734, 933
Mox20I TGGCCA 1 cut(s) 817
Mph1103I ATGCAT 1 cut(s) 1107
MroXI GAANNNNTTC 1 cut(s) 389
MscI TGGCCA 1 cut(s) 817
MseI TTAA 2 cut(s) 1011, 1067
MslI CAYNNNNRTG 2 cut(s) 299, 526
Msp20I TGGCCA 1 cut(s) 817
MspI CCGG 3 cut(s) 21, 461, 995
MspR9I CCNGG 4 cut(s) 21, 30, 184, 462
MunI CAATTG 2 cut(s) 266, 870
MvaI CCWGG 2 cut(s) 30, 184
MvnI CGCG 2 cut(s) 561, 1114
MwoI GCNNNNNNNGC 5 cut(s) 110, 485, 823, 995, 1111
NciI CCSGG 2 cut(s) 21, 462
NcoI CCATGG 1 cut(s) 312
NdeI CATATG 1 cut(s) 511
NdeII GATC 4 cut(s) 76, 80, 1036, 1084
NlaIII CATG 3 cut(s) 298, 316, 357
NlaIV GGNNCC 6 cut(s) 18, 19, 25, 627, 897, 1086
NmuCI GTSAC 1 cut(s) 517
NsiI ATGCAT 1 cut(s) 1107
NspI RCATGY 1 cut(s) 357
PaeI GCATGC 1 cut(s) 357
PaeR7I CTCGAG 1 cut(s) 657
PagI TCATGA 1 cut(s) 294
PceI AGGCCT 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 389
PfeI GAWTC 4 cut(s) 457, 931, 969, 1044
PkrI GCNGC 2 cut(s) 359, 962
PleI GAGTC 1 cut(s) 573
PpsI GAGTC 1 cut(s) 573
PsiI TTATAA 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 28, 182
PspEI GGTNACC 1 cut(s) 5
PspGI CCWGG 2 cut(s) 28, 182
PspN4I GGNNCC 6 cut(s) 18, 19, 25, 627, 897, 1086
PspOMI GGGCCC 1 cut(s) 16
PspPI GGNCC 5 cut(s) 16, 17, 43, 211, 531
PspXI VCTCGAGB 1 cut(s) 657
PstNI CAGNNNCTG 1 cut(s) 772
PsuI RGATCY 1 cut(s) 1084
RsaI GTAC 2 cut(s) 207, 956
RsaNI GTAC 2 cut(s) 206, 955
RseI CAYNNNNRTG 2 cut(s) 299, 526
SaqAI TTAA 2 cut(s) 1011, 1067
SatI GCNGC 2 cut(s) 358, 961
Sau3AI GATC 4 cut(s) 76, 80, 1036, 1084
Sau96I GGNCC 5 cut(s) 16, 17, 43, 211, 531
ScaI AGTACT 1 cut(s) 956
SchI GAGTC 1 cut(s) 574
ScrFI CCNGG 4 cut(s) 21, 30, 184, 462
SduI GDGCHC 5 cut(s) 20, 28, 450, 828, 888
SetI ASST 9 cut(s) 90, 185, 216, 627, 706, 897, 907, 1093, 1153
SexAI ACCWGGT 1 cut(s) 182
SfaNI GCATC 6 cut(s) 66, 466, 503, 846, 1086, 1114
Sfr274I CTCGAG 1 cut(s) 657
SinI GGWCC 2 cut(s) 211, 531
SlaI CTCGAG 1 cut(s) 657
SmiMI CAYNNNNRTG 2 cut(s) 299, 526
SmlI CTYRAG 3 cut(s) 167, 443, 657
SmoI CTYRAG 3 cut(s) 167, 443, 657
SphI GCATGC 1 cut(s) 357
SseBI AGGCCT 1 cut(s) 156
SsiI CCGC 4 cut(s) 357, 559, 961, 1112
SspI AATATT 1 cut(s) 981
SspMI CTAG 3 cut(s) 542, 701, 1041
StuI AGGCCT 1 cut(s) 156
StyD4I CCNGG 4 cut(s) 19, 28, 182, 460
StyI CCWWGG 2 cut(s) 312, 846
TaaI ACNGT 4 cut(s) 220, 418, 737, 823
TaqI TCGA 2 cut(s) 79, 658
TatI WGTACW 1 cut(s) 954
TauI GCSGC 2 cut(s) 360, 963
TfiI GAWTC 4 cut(s) 457, 931, 969, 1044
Tru1I TTAA 2 cut(s) 1011, 1067
Tru9I TTAA 2 cut(s) 1011, 1067
TscAI CASTG 4 cut(s) 45, 232, 1078, 1126
TseFI GTSAC 1 cut(s) 517
Tsp45I GTSAC 1 cut(s) 517
TspDTI ATGAA 4 cut(s) 493, 630, 810, 1074
TspGWI ACGGA 3 cut(s) 180, 749, 819
TspRI CASTG 4 cut(s) 45, 232, 1078, 1126
VneI GTGCAC 1 cut(s) 824
VpaK11BI GGWCC 2 cut(s) 211, 531
XapI RAATTY 3 cut(s) 617, 651, 809
XceI RCATGY 1 cut(s) 357
XcmI CCANNNNNNNNNTGG 1 cut(s) 91
XhoI CTCGAG 1 cut(s) 657
XmnI GAANNNNTTC 1 cut(s) 389
XspI CTAG 3 cut(s) 542, 701, 1041
ZrmI AGTACT 1 cut(s) 956
Zsp2I ATGCAT 1 cut(s) 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.