FvH4_6g53020

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006270
Physical Location & Seq
Reverse (-)
38921702 .. 38924004
2303 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g53020.t1

Sequence Viewer

Length: 606 bp
ATGGCAAGCCAAGCAGCTGCAACTTTTAAAGGCAACATGAAGAAAGCTGTTGCTGGCCTGAGACGGATTAATCTTGACGGGCTGCGGTGGAAAGTGTTTGATGCCAAAGGCCAGGTTCTTGGAAGGCTAGCATCTCAAATATCTACTGTGATTCAAGGCAAGGATAAACCGACTTATGCTCCAAATCGAGATGATGGAGACATGTGCATTGTGCTGAATGCGAAGGATATTTGTGTCACAGGGCGAAAACTAACTGATAAAGTTTATCATTGGCATACAGGGTATGTTGGGCACCTCAGGGAAAGGACTTTGAAGGATCAGATGGCGAAAGACCCCACAGAAGTCATTCGAAAAGCTGTATTGCGTATGCTTCCTAAGAACAAATTGCGTGACGATCGAGATAGAAAGCTGAGAATTTTTGCCGGCGGTGAACACCCATTTGGTGATAAGCCCCTTGAACCATATCTGATGCCCCCACGAAAAGTAAGGGAAATGCGACCTCGTACAAGACGAGCCTTGGTAAGAGCTCAGATAAAGGCTGAAAGCCAGGATGATAAAGATTCACGAAAGGGCAGGAAGAAGGATAGAGTCGAAGTGAATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L13

Protein Analysis

202

Amino Acids

23.01

Weight (kDa)

10.34

Isoelectric Point (pI)

31.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L13 PF00572 29 - 146 2.2e-44 Ribosomal protein L13
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 291
AciI CCGC 2 cut(s) 85, 426
AclWI GGATC 1 cut(s) 324
AcsI RAATTY 1 cut(s) 414
AfaI GTAC 1 cut(s) 505
AflIII ACRYGT 1 cut(s) 201
AgsI TTSAA 3 cut(s) 155, 313, 458
AjnI CCWGG 2 cut(s) 111, 546
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
AluBI AGCT 5 cut(s) 17, 47, 356, 409, 527
AluI AGCT 5 cut(s) 17, 47, 356, 409, 527
Alw21I GWGCWC 1 cut(s) 529
Alw26I GTCTC 2 cut(s) 55, 192
AlwI GGATC 1 cut(s) 324
AoxI GGCC 2 cut(s) 55, 109
ApeKI GCWGC 3 cut(s) 14, 17, 82
ApoI RAATTY 1 cut(s) 414
AseI ATTAAT 1 cut(s) 69
Asp700I GAANNNNTTC 1 cut(s) 345
AsuHPI GGTGA 2 cut(s) 440, 455
AsuII TTCGAA 1 cut(s) 349
AsuNHI GCTAGC 1 cut(s) 127
AxyI CCTNAGG 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 294
BanI GGYRCC 1 cut(s) 291
BanII GRGCYC 1 cut(s) 529
Bbv12I GWGCWC 1 cut(s) 529
BbvI GCAGC 3 cut(s) 4, 26, 69
BccI CCATC 2 cut(s) 188, 316
BciT130I CCWGG 2 cut(s) 113, 548
BcoDI GTCTC 2 cut(s) 55, 192
BfaI CTAG 1 cut(s) 128
BisI GCNGC 3 cut(s) 15, 18, 83
BlsI GCNGC 3 cut(s) 16, 19, 84
Bme1390I CCNGG 2 cut(s) 113, 548
BmiI GGNNCC 1 cut(s) 293
BmrFI CCNGG 2 cut(s) 113, 548
BmsI GCATC 3 cut(s) 91, 140, 459
BmtI GCTAGC 1 cut(s) 131
Bpu14I TTCGAA 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 516
BsaXI ACNNNNNCTCC 2 cut(s) 163, 193
Bse118I RCCGGY 1 cut(s) 422
Bse21I CCTNAGG 1 cut(s) 296
BseBI CCWGG 2 cut(s) 113, 548
BseDI CCNNGG 1 cut(s) 516
BseGI GGATG 1 cut(s) 556
BseMII CTCAG 4 cut(s) 50, 310, 401, 542
BseSI GKGCMC 1 cut(s) 294
BseXI GCAGC 3 cut(s) 4, 26, 69
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 2 cut(s) 57, 111
BshNI GGYRCC 1 cut(s) 291
BsiEI CGRYCG 1 cut(s) 397
BsiHKAI GWGCWC 1 cut(s) 529
BsiSI CCGG 1 cut(s) 423
BsmAI GTCTC 2 cut(s) 55, 192
BsmBI CGTCTC 1 cut(s) 55
BsmI GAATGC 1 cut(s) 223
BsnI GGCC 2 cut(s) 57, 111
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 2 cut(s) 294, 529
Bsp143I GATC 2 cut(s) 316, 394
BspACI CCGC 2 cut(s) 85, 426
BspANI GGCC 2 cut(s) 57, 111
BspCNI CTCAG 4 cut(s) 51, 309, 402, 541
BspLI GGNNCC 1 cut(s) 293
BspOI GCTAGC 1 cut(s) 131
BspPI GGATC 1 cut(s) 324
BspT104I TTCGAA 1 cut(s) 349
BspT107I GGYRCC 1 cut(s) 291
BsrFI RCCGGY 1 cut(s) 422
BssAI RCCGGY 1 cut(s) 422
BssECI CCNNGG 1 cut(s) 516
BssMI GATC 2 cut(s) 316, 394
BssT1I CCWWGG 1 cut(s) 516
Bst2UI CCWGG 2 cut(s) 113, 548
Bst4CI ACNGT 1 cut(s) 148
BstBI TTCGAA 1 cut(s) 349
BstC8I GCNNGC 4 cut(s) 7, 55, 129, 424
BstDEI CTNAG 5 cut(s) 59, 296, 375, 410, 528
BstF5I GGATG 1 cut(s) 556
BstKTI GATC 2 cut(s) 319, 397
BstMAI GTCTC 2 cut(s) 55, 192
BstMBI GATC 2 cut(s) 316, 394
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 113, 548
BstNSI RCATGY 1 cut(s) 205
BstSCI CCNGG 2 cut(s) 111, 546
BstSLI GKGCMC 1 cut(s) 294
BstV1I GCAGC 3 cut(s) 4, 26, 69
BstXI CCANNNNNNTGG 1 cut(s) 119
Bsu36I CCTNAGG 1 cut(s) 296
BsuRI GGCC 2 cut(s) 57, 111
BtsCI GGATG 1 cut(s) 556
Cac8I GCNNGC 4 cut(s) 7, 55, 129, 424
Cfr10I RCCGGY 1 cut(s) 422
Csp6I GTAC 1 cut(s) 504
CviAII CATG 2 cut(s) 37, 202
CviQI GTAC 1 cut(s) 504
DdeI CTNAG 5 cut(s) 59, 296, 375, 410, 528
DpnI GATC 2 cut(s) 318, 396
DpnII GATC 2 cut(s) 316, 394
DraI TTTAAA 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 527
Eco130I CCWWGG 1 cut(s) 516
Eco24I GRGCYC 1 cut(s) 529
Eco53kI GAGCTC 1 cut(s) 527
Eco81I CCTNAGG 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 527
EcoRII CCWGG 2 cut(s) 111, 546
EcoT14I CCWWGG 1 cut(s) 516
EcoT38I GRGCYC 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 516
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 2 cut(s) 40, 205
FaiI YATR 8 cut(s) 38, 177, 203, 276, 285, 368, 463, 604
FatI CATG 2 cut(s) 36, 201
Fnu4HI GCNGC 3 cut(s) 15, 18, 83
FokI GGATG 1 cut(s) 563
FriOI GRGCYC 1 cut(s) 529
Fsp4HI GCNGC 3 cut(s) 15, 18, 83
FspBI CTAG 1 cut(s) 128
GluI GCNGC 3 cut(s) 15, 18, 83
HaeIII GGCC 2 cut(s) 57, 111
HapII CCGG 1 cut(s) 423
Hin1II CATG 2 cut(s) 40, 205
HinfI GANTC 3 cut(s) 151, 560, 588
HpaII CCGG 1 cut(s) 423
HphI GGTGA 2 cut(s) 440, 455
Hpy166II GTNNAC 1 cut(s) 431
Hpy188I TCNGA 3 cut(s) 321, 468, 531
Hpy188III TCNNGA 4 cut(s) 74, 188, 398, 564
Hpy8I GTNNAC 1 cut(s) 431
HpyAV CCTTC 4 cut(s) 117, 217, 307, 574
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4V TGCA 2 cut(s) 20, 207
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 5 cut(s) 59, 296, 375, 410, 528
Hsp92II CATG 2 cut(s) 40, 205
KroI GCCGGC 1 cut(s) 422
KroNI GCCGGC 1 cut(s) 424
Kzo9I GATC 2 cut(s) 316, 394
LmnI GCTCC 1 cut(s) 184
Lsp1109I GCAGC 3 cut(s) 4, 26, 69
LweI GCATC 3 cut(s) 91, 140, 459
MaeI CTAG 1 cut(s) 128
MaeIII GTNAC 2 cut(s) 235, 389
MalI GATC 2 cut(s) 318, 396
MboI GATC 2 cut(s) 316, 394
MboII GAAGA 2 cut(s) 52, 589
MhlI GDGCHC 2 cut(s) 294, 529
MluCI AATT 2 cut(s) 383, 414
MlyI GAGTC 1 cut(s) 597
MnlI CCTC 2 cut(s) 305, 510
MroNI GCCGGC 1 cut(s) 422
MroXI GAANNNNTTC 1 cut(s) 345
MseI TTAA 2 cut(s) 27, 69
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 1 cut(s) 423
MspR9I CCNGG 2 cut(s) 113, 548
Mva1269I GAATGC 1 cut(s) 223
MvaI CCWGG 2 cut(s) 113, 548
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 424
NdeII GATC 2 cut(s) 316, 394
NgoMIV GCCGGC 1 cut(s) 422
NheI GCTAGC 1 cut(s) 127
NlaIII CATG 2 cut(s) 40, 205
NlaIV GGNNCC 1 cut(s) 293
NmuCI GTSAC 2 cut(s) 235, 389
NspI RCATGY 1 cut(s) 205
NspV TTCGAA 1 cut(s) 349
PciI ACATGT 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 508
PctI GAATGC 1 cut(s) 223
PdiI GCCGGC 1 cut(s) 424
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 2 cut(s) 151, 560
PkrI GCNGC 3 cut(s) 16, 19, 84
Ple19I CGATCG 1 cut(s) 397
PleI GAGTC 1 cut(s) 596
PpsI GAGTC 1 cut(s) 596
PscI ACATGT 1 cut(s) 201
PshBI ATTAAT 1 cut(s) 69
Psp124BI GAGCTC 1 cut(s) 529
Psp6I CCWGG 2 cut(s) 111, 546
PspGI CCWGG 2 cut(s) 111, 546
PspN4I GGNNCC 1 cut(s) 293
PvuI CGATCG 1 cut(s) 397
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
SacI GAGCTC 1 cut(s) 529
SaqAI TTAA 2 cut(s) 27, 69
SatI GCNGC 3 cut(s) 15, 18, 83
Sau3AI GATC 2 cut(s) 316, 394
SchI GAGTC 1 cut(s) 597
ScrFI CCNGG 2 cut(s) 113, 548
SduI GDGCHC 2 cut(s) 294, 529
SetI ASST 8 cut(s) 19, 49, 117, 297, 358, 411, 502, 529
SfaNI GCATC 3 cut(s) 91, 140, 459
SfuI TTCGAA 1 cut(s) 349
Sse9I AATT 2 cut(s) 383, 414
SsiI CCGC 2 cut(s) 85, 426
SspMI CTAG 1 cut(s) 128
SstI GAGCTC 1 cut(s) 529
StyD4I CCNGG 2 cut(s) 111, 546
StyI CCWWGG 1 cut(s) 516
TaaI ACNGT 1 cut(s) 148
TaqI TCGA 4 cut(s) 187, 349, 397, 591
TasI AATT 2 cut(s) 383, 414
TfiI GAWTC 2 cut(s) 151, 560
Tru1I TTAA 2 cut(s) 27, 69
Tru9I TTAA 2 cut(s) 27, 69
TseFI GTSAC 2 cut(s) 235, 389
TseI GCWGC 3 cut(s) 14, 17, 82
Tsp45I GTSAC 2 cut(s) 235, 389
TspDTI ATGAA 1 cut(s) 53
TspGWI ACGGA 1 cut(s) 79
VspI ATTAAT 1 cut(s) 69
XapI RAATTY 1 cut(s) 414
XceI RCATGY 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 345
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.