Rh4CG165000

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0006270
Physical Location & Seq
Reverse (-)
36422327 .. 36426045
3719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG165000.1

Sequence Viewer

Length: 657 bp
ATGGCAAGCCAGGCAGCTAGGTCTTTTAACGGTAACATGAAGAAAGCTGTTGCCGGCCTCAGACGTATTAATCTTGATGGTCTGAGATGGAGAGTATTCGATGCGAAAGGCCAGGTTCTTGGAAGGCTAGCATCTCAAATATCTACTGTGATTCAAGGAAAGGATAAACCAACATACACTCCAAATCGAGATGATGGAGATATGTGCATTGTGCTGAACGCGAAGGATATTTGCGTCACGGGTAGTAAACTGACTGATAAAGTTTATCATTGGCATACTGGGTATGTGGGTCATCTGAAGGAGAGGACGTTAAAAGATCAGATGGTCAAAGATCCTACAGAAGTCATTCGCAAAGCTGTGTTACGTATGCTTCCAAAGAACAAATTGCGCGATGATAGAGATAGAAAGCTGAGGATATTTCCTGGAAGTGAACACCCCTTTGGTGATCGGCCCCTTGAACCATATGTGATGCCCCCTAGGAGAGTAAGGGAAATGCGGCCTCGTACAAGACGAGCCATTGTCCGAGCTCAGAAAAAGGCTGAACAGCAACAACAAGATGCTAATGATCCACGAAAGGGCAAAAAGAAGGATACACGGGAATTGGTTGCTTCTAATTACTCGAGCTTATTTGGGCTTATGAAAGTTGTTGAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L13

Protein Analysis

218

Amino Acids

25.05

Weight (kDa)

10.28

Isoelectric Point (pI)

34.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L13 PF00572 29 - 146 4.9e-45 Ribosomal protein L13
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 221, 390
AciI CCGC 1 cut(s) 496
AclWI GGATC 2 cut(s) 326, 560
AcuI CTGAAG 1 cut(s) 317
AfaI GTAC 1 cut(s) 505
AfiI CCNNNNNNNGG 1 cut(s) 575
AgsI TTSAA 2 cut(s) 155, 458
AjnI CCWGG 3 cut(s) 9, 111, 421
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
AluBI AGCT 6 cut(s) 17, 47, 356, 409, 527, 624
AluI AGCT 6 cut(s) 17, 47, 356, 409, 527, 624
Alw21I GWGCWC 1 cut(s) 529
AlwI GGATC 2 cut(s) 326, 560
Ama87I CYCGRG 1 cut(s) 619
AoxI GGCC 4 cut(s) 55, 109, 449, 497
ApeKI GCWGC 1 cut(s) 14
AseI ATTAAT 1 cut(s) 69
Asp700I GAANNNNTTC 1 cut(s) 345
AspA2I CCTAGG 1 cut(s) 476
AspLEI GCGC 1 cut(s) 390
AspS9I GGNCC 1 cut(s) 450
AsuHPI GGTGA 1 cut(s) 455
AsuNHI GCTAGC 1 cut(s) 127
AvaI CYCGRG 1 cut(s) 619
AvrII CCTAGG 1 cut(s) 476
BanII GRGCYC 1 cut(s) 529
Bbv12I GWGCWC 1 cut(s) 529
BbvCI CCTCAGC 1 cut(s) 410
BbvI GCAGC 1 cut(s) 26
BccI CCATC 4 cut(s) 71, 81, 188, 316
BciT130I CCWGG 3 cut(s) 11, 113, 423
BciVI GTATCC 1 cut(s) 583
BfaI CTAG 3 cut(s) 18, 128, 477
BfmI CTRYAG 1 cut(s) 336
BfuI GTATCC 1 cut(s) 583
BisI GCNGC 2 cut(s) 15, 497
BlnI CCTAGG 1 cut(s) 476
BlsI GCNGC 2 cut(s) 16, 498
Bme1390I CCNGG 3 cut(s) 11, 113, 423
BmeT110I CYCGRG 1 cut(s) 619
BmgT120I GGNCC 1 cut(s) 450
BmiI GGNNCC 1 cut(s) 452
BmrFI CCNGG 3 cut(s) 11, 113, 423
BmrI ACTGGG 1 cut(s) 288
BmsI GCATC 4 cut(s) 91, 140, 459, 547
BmtI GCTAGC 1 cut(s) 131
BmuI ACTGGG 1 cut(s) 288
Bpu10I CCTNAGC 1 cut(s) 410
BsaAI YACGTR 1 cut(s) 365
BsaJI CCNNGG 1 cut(s) 476
BsaXI ACNNNNNCTCC 4 cut(s) 163, 193, 293, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 575
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 1 cut(s) 283
BseBI CCWGG 3 cut(s) 11, 113, 423
BseDI CCNNGG 1 cut(s) 476
BseLI CCNNNNNNNGG 1 cut(s) 575
BseMII CTCAG 4 cut(s) 73, 74, 401, 542
BseNI ACTGG 1 cut(s) 283
BseXI GCAGC 1 cut(s) 26
Bsh1236I CGCG 2 cut(s) 221, 390
BshFI GGCC 4 cut(s) 57, 111, 451, 499
BsiHKAI GWGCWC 1 cut(s) 529
BsiHKCI CYCGRG 1 cut(s) 619
BsiSI CCGG 1 cut(s) 54
BslI CCNNNNNNNGG 1 cut(s) 575
BsnI GGCC 4 cut(s) 57, 111, 451, 499
BsoBI CYCGRG 1 cut(s) 619
Bsp1286I GDGCHC 1 cut(s) 529
Bsp143I GATC 4 cut(s) 316, 331, 445, 565
BspACI CCGC 1 cut(s) 496
BspANI GGCC 4 cut(s) 57, 111, 451, 499
BspCNI CTCAG 4 cut(s) 72, 75, 402, 541
BspFNI CGCG 2 cut(s) 221, 390
BspLI GGNNCC 1 cut(s) 452
BspOI GCTAGC 1 cut(s) 131
BspPI GGATC 2 cut(s) 326, 560
BsrFI RCCGGY 1 cut(s) 53
BsrI ACTGG 1 cut(s) 283
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 476
BssMI GATC 4 cut(s) 316, 331, 445, 565
BssT1I CCWWGG 1 cut(s) 476
Bst2UI CCWGG 3 cut(s) 11, 113, 423
Bst4CI ACNGT 2 cut(s) 32, 148
BstBAI YACGTR 1 cut(s) 365
BstC8I GCNNGC 3 cut(s) 7, 55, 129
BstDEI CTNAG 4 cut(s) 59, 83, 410, 528
BstFNI CGCG 2 cut(s) 221, 390
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 4 cut(s) 319, 334, 448, 568
BstMBI GATC 4 cut(s) 316, 331, 445, 565
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 3 cut(s) 11, 113, 423
BstSCI CCNGG 3 cut(s) 9, 111, 421
BstSFI CTRYAG 1 cut(s) 336
BstSNI TACGTA 1 cut(s) 365
BstUI CGCG 2 cut(s) 221, 390
BstV1I GCAGC 1 cut(s) 26
BstX2I RGATCY 1 cut(s) 331
BstXI CCANNNNNNTGG 1 cut(s) 119
BstYI RGATCY 1 cut(s) 331
BsuI GTATCC 1 cut(s) 583
BsuRI GGCC 4 cut(s) 57, 111, 451, 499
BtgZI GCGATG 1 cut(s) 405
Cac8I GCNNGC 3 cut(s) 7, 55, 129
CfoI GCGC 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 450
CseI GACGC 1 cut(s) 223
Csp6I GTAC 1 cut(s) 504
CviAII CATG 1 cut(s) 37
CviQI GTAC 1 cut(s) 504
DdeI CTNAG 4 cut(s) 59, 83, 410, 528
DpnI GATC 4 cut(s) 318, 333, 447, 567
DpnII GATC 4 cut(s) 316, 331, 445, 565
Ecl136II GAGCTC 1 cut(s) 527
Eco105I TACGTA 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 476
Eco24I GRGCYC 1 cut(s) 529
Eco53kI GAGCTC 1 cut(s) 527
Eco57I CTGAAG 1 cut(s) 317
Eco88I CYCGRG 1 cut(s) 619
EcoICRI GAGCTC 1 cut(s) 527
EcoRII CCWGG 3 cut(s) 9, 111, 421
EcoT14I CCWWGG 1 cut(s) 476
EcoT38I GRGCYC 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 476
FaeI CATG 1 cut(s) 40
FaiI YATR 9 cut(s) 38, 175, 203, 276, 285, 368, 463, 465, 638
FatI CATG 1 cut(s) 36
FauNDI CATATG 1 cut(s) 463
Fnu4HI GCNGC 2 cut(s) 15, 497
FriOI GRGCYC 1 cut(s) 529
Fsp4HI GCNGC 2 cut(s) 15, 497
FspBI CTAG 3 cut(s) 18, 128, 477
GlaI GCGC 1 cut(s) 389
GluI GCNGC 2 cut(s) 15, 497
HaeIII GGCC 4 cut(s) 57, 111, 451, 499
HapII CCGG 1 cut(s) 54
HgaI GACGC 1 cut(s) 223
HhaI GCGC 1 cut(s) 390
Hin1II CATG 1 cut(s) 40
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HinfI GANTC 1 cut(s) 151
HpaII CCGG 1 cut(s) 54
HphI GGTGA 1 cut(s) 455
Hpy166II GTNNAC 2 cut(s) 248, 431
Hpy188I TCNGA 6 cut(s) 62, 84, 297, 321, 524, 531
Hpy188III TCNNGA 2 cut(s) 74, 188
Hpy8I GTNNAC 2 cut(s) 248, 431
HpyAV CCTTC 4 cut(s) 117, 217, 292, 580
HpyCH4III ACNGT 2 cut(s) 32, 148
HpyCH4IV ACGT 3 cut(s) 64, 308, 364
HpyCH4V TGCA 1 cut(s) 207
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 4 cut(s) 59, 83, 410, 528
HpySE526I ACGT 3 cut(s) 64, 308, 364
Hsp92II CATG 1 cut(s) 40
HspAI GCGC 1 cut(s) 388
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 4 cut(s) 316, 331, 445, 565
LpnPI CCDG 7 cut(s) 23, 67, 98, 125, 264, 408, 435
Lsp1109I GCAGC 1 cut(s) 26
LweI GCATC 4 cut(s) 91, 140, 459, 547
MaeI CTAG 3 cut(s) 18, 128, 477
MaeII ACGT 3 cut(s) 64, 308, 364
MaeIII GTNAC 3 cut(s) 32, 235, 360
MalI GATC 4 cut(s) 318, 333, 447, 567
MboI GATC 4 cut(s) 316, 331, 445, 565
MboII GAAGA 1 cut(s) 52
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 1 cut(s) 529
MluCI AATT 3 cut(s) 383, 599, 613
MnlI CCTC 5 cut(s) 68, 297, 405, 510, 643
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 345
MseI TTAA 3 cut(s) 27, 69, 311
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 3 cut(s) 11, 113, 423
MvaI CCWGG 3 cut(s) 11, 113, 423
MvnI CGCG 2 cut(s) 221, 390
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 55
NdeI CATATG 1 cut(s) 463
NdeII GATC 4 cut(s) 316, 331, 445, 565
NgoMIV GCCGGC 1 cut(s) 53
NheI GCTAGC 1 cut(s) 127
NlaIII CATG 1 cut(s) 40
NlaIV GGNNCC 1 cut(s) 452
NmuCI GTSAC 1 cut(s) 235
PaeR7I CTCGAG 1 cut(s) 619
PcsI WCGNNNNNNNCGW 1 cut(s) 508
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 1 cut(s) 151
PfoI TCCNGGA 1 cut(s) 421
PkrI GCNGC 2 cut(s) 16, 498
Ppu21I YACGTR 1 cut(s) 365
PshBI ATTAAT 1 cut(s) 69
Psp124BI GAGCTC 1 cut(s) 529
Psp6I CCWGG 3 cut(s) 9, 111, 421
PspGI CCWGG 3 cut(s) 9, 111, 421
PspN4I GGNNCC 1 cut(s) 452
PspPI GGNCC 1 cut(s) 450
PspXI VCTCGAGB 1 cut(s) 619
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
SacI GAGCTC 1 cut(s) 529
SaqAI TTAA 3 cut(s) 27, 69, 311
SatI GCNGC 2 cut(s) 15, 497
Sau3AI GATC 4 cut(s) 316, 331, 445, 565
Sau96I GGNCC 1 cut(s) 450
ScrFI CCNGG 3 cut(s) 11, 113, 423
SduI GDGCHC 1 cut(s) 529
SfaNI GCATC 4 cut(s) 91, 140, 459, 547
SfcI CTRYAG 1 cut(s) 336
Sfr274I CTCGAG 1 cut(s) 619
SlaI CTCGAG 1 cut(s) 619
SmlI CTYRAG 1 cut(s) 619
SmoI CTYRAG 1 cut(s) 619
SnaBI TACGTA 1 cut(s) 365
Sse9I AATT 3 cut(s) 383, 599, 613
SsiI CCGC 1 cut(s) 496
SspMI CTAG 3 cut(s) 18, 128, 477
SstI GAGCTC 1 cut(s) 529
StyD4I CCNGG 3 cut(s) 9, 111, 421
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 2 cut(s) 32, 148
TaiI ACGT 3 cut(s) 67, 311, 367
TaqI TCGA 3 cut(s) 99, 187, 620
TasI AATT 3 cut(s) 383, 599, 613
TauI GCSGC 1 cut(s) 499
TfiI GAWTC 1 cut(s) 151
Tru1I TTAA 3 cut(s) 27, 69, 311
Tru9I TTAA 3 cut(s) 27, 69, 311
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 2 cut(s) 53, 653
VspI ATTAAT 1 cut(s) 69
XhoI CTCGAG 1 cut(s) 619
XmaJI CCTAGG 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 345
XspI CTAG 3 cut(s) 18, 128, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.