Rw4G012750

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006270
Physical Location & Seq
Reverse (-)
29947694 .. 29949645
1952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G012750.1

Sequence Viewer

Length: 684 bp
ATGGCAAGCCAGGCAGCTAGGTCTTTTAACGGTAACATGAAGGTGATGTTGCCTTCTTGTGCTTTGATGCTGTTGCCTTGCCGGCTCAGACGTATTAATCTTGATGGTCTGAGATGGAGAGTATTCGATGCGAAAGGCCAGGTTCTTGGAAGGCTAGCATCTCAAATATCTACTGTGATTCAAGGAAAGGATAAACCAACATACACTCCAAATCGAGATGATGGAGATATGTGCATTGTGCTGAACGCGAAGGATATTTGCGTCACGGGTAGTAAACTGACTGATAAAGTTTATCATTGGCATACTGGGTATGTGGGTCATCTGAAGGAGAGGACGTTAAAAGATCAGATGGTCAAAGATCCTACAGAAGTCATTCGCAAAGCTGTGTTACGTATGCTTCCAAAGAACAAATTGCGCGATGATAGAGATAGAAAGCTGAGGATATTTCCTGGAAGTGAACACCCCTTTGGTGATCGGCCCCTTGAACCATATGTGATGCCCCCTAGGAGAGTAAGGGAAATGCGGCCCCGTACAAGACGAGCCATTGTCCGAGCTCAGAAAAAGGCTGAACAGCAACAACAAGATGCTAATGATCCACGAAAGGGCAAAAAGAAGGATACACGGGAATTGGTTGCTTCTAATTACTCGAGCTTATTTGGGCTTATGAAAGTTGTTGAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L13

Protein Analysis

227

Amino Acids

26.15

Weight (kDa)

10.19

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L13 PF00572 38 - 155 5.4e-45 Ribosomal protein L13
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 248, 417
AciI CCGC 1 cut(s) 523
AclWI GGATC 2 cut(s) 353, 587
AcuI CTGAAG 1 cut(s) 344
AfaI GTAC 1 cut(s) 532
AfiI CCNNNNNNNGG 1 cut(s) 602
AgsI TTSAA 2 cut(s) 182, 485
AjnI CCWGG 3 cut(s) 9, 138, 448
AjuI GAANNNNNNNTTGG 2 cut(s) 450, 482
AluBI AGCT 5 cut(s) 17, 383, 436, 554, 651
AluI AGCT 5 cut(s) 17, 383, 436, 554, 651
Alw21I GWGCWC 1 cut(s) 556
AlwI GGATC 2 cut(s) 353, 587
Ama87I CYCGRG 1 cut(s) 646
AoxI GGCC 3 cut(s) 136, 476, 524
ApeKI GCWGC 1 cut(s) 14
AseI ATTAAT 1 cut(s) 96
Asp700I GAANNNNTTC 1 cut(s) 372
AspA2I CCTAGG 1 cut(s) 503
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 2 cut(s) 477, 525
AsuHPI GGTGA 2 cut(s) 55, 482
AsuNHI GCTAGC 1 cut(s) 154
AvaI CYCGRG 1 cut(s) 646
AvrII CCTAGG 1 cut(s) 503
BanII GRGCYC 1 cut(s) 556
Bbv12I GWGCWC 1 cut(s) 556
BbvCI CCTCAGC 1 cut(s) 437
BbvI GCAGC 1 cut(s) 26
BccI CCATC 4 cut(s) 98, 108, 215, 343
BciT130I CCWGG 3 cut(s) 11, 140, 450
BciVI GTATCC 1 cut(s) 610
BfaI CTAG 3 cut(s) 18, 155, 504
BfmI CTRYAG 1 cut(s) 363
BfuI GTATCC 1 cut(s) 610
BglI GCCNNNNNGGC 1 cut(s) 82
BisI GCNGC 2 cut(s) 15, 524
BlnI CCTAGG 1 cut(s) 503
BlsI GCNGC 2 cut(s) 16, 525
Bme1390I CCNGG 3 cut(s) 11, 140, 450
BmeT110I CYCGRG 1 cut(s) 646
BmgT120I GGNCC 2 cut(s) 477, 525
BmiI GGNNCC 2 cut(s) 479, 527
BmrFI CCNGG 3 cut(s) 11, 140, 450
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 5 cut(s) 57, 118, 167, 486, 574
BmtI GCTAGC 1 cut(s) 158
BmuI ACTGGG 1 cut(s) 315
Bpu10I CCTNAGC 1 cut(s) 437
BsaAI YACGTR 1 cut(s) 392
BsaJI CCNNGG 1 cut(s) 503
BsaXI ACNNNNNCTCC 4 cut(s) 190, 220, 320, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 602
Bse118I RCCGGY 1 cut(s) 81
Bse1I ACTGG 1 cut(s) 310
BseBI CCWGG 3 cut(s) 11, 140, 450
BseDI CCNNGG 1 cut(s) 503
BseLI CCNNNNNNNGG 1 cut(s) 602
BseMII CTCAG 4 cut(s) 100, 101, 428, 569
BseNI ACTGG 1 cut(s) 310
BseXI GCAGC 1 cut(s) 26
Bsh1236I CGCG 2 cut(s) 248, 417
BshFI GGCC 3 cut(s) 138, 478, 526
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 1 cut(s) 646
BsiSI CCGG 1 cut(s) 82
BslI CCNNNNNNNGG 1 cut(s) 602
BsnI GGCC 3 cut(s) 138, 478, 526
BsoBI CYCGRG 1 cut(s) 646
Bsp1286I GDGCHC 1 cut(s) 556
Bsp143I GATC 4 cut(s) 343, 358, 472, 592
BspACI CCGC 1 cut(s) 523
BspANI GGCC 3 cut(s) 138, 478, 526
BspCNI CTCAG 4 cut(s) 99, 102, 429, 568
BspFNI CGCG 2 cut(s) 248, 417
BspLI GGNNCC 2 cut(s) 479, 527
BspOI GCTAGC 1 cut(s) 158
BspPI GGATC 2 cut(s) 353, 587
BsrFI RCCGGY 1 cut(s) 81
BsrI ACTGG 1 cut(s) 310
BssAI RCCGGY 1 cut(s) 81
BssECI CCNNGG 1 cut(s) 503
BssMI GATC 4 cut(s) 343, 358, 472, 592
BssT1I CCWWGG 1 cut(s) 503
Bst2UI CCWGG 3 cut(s) 11, 140, 450
Bst4CI ACNGT 2 cut(s) 32, 175
BstBAI YACGTR 1 cut(s) 392
BstC8I GCNNGC 3 cut(s) 7, 83, 156
BstDEI CTNAG 4 cut(s) 86, 110, 437, 555
BstFNI CGCG 2 cut(s) 248, 417
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 4 cut(s) 346, 361, 475, 595
BstMBI GATC 4 cut(s) 343, 358, 472, 592
BstMWI GCNNNNNNNGC 2 cut(s) 11, 82
BstNI CCWGG 3 cut(s) 11, 140, 450
BstSCI CCNGG 3 cut(s) 9, 138, 448
BstSFI CTRYAG 1 cut(s) 363
BstSNI TACGTA 1 cut(s) 392
BstUI CGCG 2 cut(s) 248, 417
BstV1I GCAGC 1 cut(s) 26
BstX2I RGATCY 1 cut(s) 358
BstXI CCANNNNNNTGG 1 cut(s) 146
BstYI RGATCY 1 cut(s) 358
BsuI GTATCC 1 cut(s) 610
BsuRI GGCC 3 cut(s) 138, 478, 526
BtgZI GCGATG 1 cut(s) 432
Cac8I GCNNGC 3 cut(s) 7, 83, 156
CfoI GCGC 1 cut(s) 417
Cfr10I RCCGGY 1 cut(s) 81
Cfr13I GGNCC 2 cut(s) 477, 525
CseI GACGC 1 cut(s) 250
Csp6I GTAC 1 cut(s) 531
CviAII CATG 1 cut(s) 37
CviQI GTAC 1 cut(s) 531
DdeI CTNAG 4 cut(s) 86, 110, 437, 555
DpnI GATC 4 cut(s) 345, 360, 474, 594
DpnII GATC 4 cut(s) 343, 358, 472, 592
Ecl136II GAGCTC 1 cut(s) 554
Eco105I TACGTA 1 cut(s) 392
Eco130I CCWWGG 1 cut(s) 503
Eco24I GRGCYC 1 cut(s) 556
Eco53kI GAGCTC 1 cut(s) 554
Eco57I CTGAAG 1 cut(s) 344
Eco88I CYCGRG 1 cut(s) 646
EcoICRI GAGCTC 1 cut(s) 554
EcoRII CCWGG 3 cut(s) 9, 138, 448
EcoT14I CCWWGG 1 cut(s) 503
EcoT38I GRGCYC 1 cut(s) 556
ErhI CCWWGG 1 cut(s) 503
FaeI CATG 1 cut(s) 40
FaiI YATR 9 cut(s) 38, 202, 230, 303, 312, 395, 490, 492, 665
FatI CATG 1 cut(s) 36
FauNDI CATATG 1 cut(s) 490
Fnu4HI GCNGC 2 cut(s) 15, 524
FriOI GRGCYC 1 cut(s) 556
Fsp4HI GCNGC 2 cut(s) 15, 524
FspBI CTAG 3 cut(s) 18, 155, 504
GlaI GCGC 1 cut(s) 416
GluI GCNGC 2 cut(s) 15, 524
HaeIII GGCC 3 cut(s) 138, 478, 526
HapII CCGG 1 cut(s) 82
HgaI GACGC 1 cut(s) 250
HhaI GCGC 1 cut(s) 417
Hin1II CATG 1 cut(s) 40
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HinfI GANTC 1 cut(s) 178
HpaII CCGG 1 cut(s) 82
HphI GGTGA 2 cut(s) 55, 482
Hpy166II GTNNAC 2 cut(s) 275, 458
Hpy188I TCNGA 6 cut(s) 89, 111, 324, 348, 551, 558
Hpy188III TCNNGA 2 cut(s) 101, 215
Hpy8I GTNNAC 2 cut(s) 275, 458
HpyAV CCTTC 6 cut(s) 34, 63, 144, 244, 319, 607
HpyCH4III ACNGT 2 cut(s) 32, 175
HpyCH4IV ACGT 3 cut(s) 91, 335, 391
HpyCH4V TGCA 1 cut(s) 234
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 82
HpyF3I CTNAG 4 cut(s) 86, 110, 437, 555
HpySE526I ACGT 3 cut(s) 91, 335, 391
Hsp92II CATG 1 cut(s) 40
HspAI GCGC 1 cut(s) 415
KroI GCCGGC 1 cut(s) 81
KroNI GCCGGC 1 cut(s) 83
Kzo9I GATC 4 cut(s) 343, 358, 472, 592
LpnPI CCDG 7 cut(s) 23, 95, 125, 152, 291, 435, 462
Lsp1109I GCAGC 1 cut(s) 26
LweI GCATC 5 cut(s) 57, 118, 167, 486, 574
MaeI CTAG 3 cut(s) 18, 155, 504
MaeII ACGT 3 cut(s) 91, 335, 391
MaeIII GTNAC 3 cut(s) 32, 262, 387
MalI GATC 4 cut(s) 345, 360, 474, 594
MboI GATC 4 cut(s) 343, 358, 472, 592
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 556
MluCI AATT 3 cut(s) 410, 626, 640
MnlI CCTC 3 cut(s) 324, 432, 670
MroNI GCCGGC 1 cut(s) 81
MroXI GAANNNNTTC 1 cut(s) 372
MseI TTAA 3 cut(s) 27, 96, 338
MslI CAYNNNNRTG 1 cut(s) 41
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 3 cut(s) 11, 140, 450
MvaI CCWGG 3 cut(s) 11, 140, 450
MvnI CGCG 2 cut(s) 248, 417
MwoI GCNNNNNNNGC 2 cut(s) 11, 82
NaeI GCCGGC 1 cut(s) 83
NdeI CATATG 1 cut(s) 490
NdeII GATC 4 cut(s) 343, 358, 472, 592
NgoMIV GCCGGC 1 cut(s) 81
NheI GCTAGC 1 cut(s) 154
NlaIII CATG 1 cut(s) 40
NlaIV GGNNCC 2 cut(s) 479, 527
NmuCI GTSAC 1 cut(s) 262
PaeR7I CTCGAG 1 cut(s) 646
PdiI GCCGGC 1 cut(s) 83
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 1 cut(s) 178
PfoI TCCNGGA 1 cut(s) 448
PkrI GCNGC 2 cut(s) 16, 525
Ppu21I YACGTR 1 cut(s) 392
PshBI ATTAAT 1 cut(s) 96
Psp124BI GAGCTC 1 cut(s) 556
Psp6I CCWGG 3 cut(s) 9, 138, 448
PspGI CCWGG 3 cut(s) 9, 138, 448
PspN4I GGNNCC 2 cut(s) 479, 527
PspPI GGNCC 2 cut(s) 477, 525
PspXI VCTCGAGB 1 cut(s) 646
PsuI RGATCY 1 cut(s) 358
RsaI GTAC 1 cut(s) 532
RsaNI GTAC 1 cut(s) 531
RseI CAYNNNNRTG 1 cut(s) 41
SacI GAGCTC 1 cut(s) 556
SaqAI TTAA 3 cut(s) 27, 96, 338
SatI GCNGC 2 cut(s) 15, 524
Sau3AI GATC 4 cut(s) 343, 358, 472, 592
Sau96I GGNCC 2 cut(s) 477, 525
ScrFI CCNGG 3 cut(s) 11, 140, 450
SduI GDGCHC 1 cut(s) 556
SfaNI GCATC 5 cut(s) 57, 118, 167, 486, 574
SfcI CTRYAG 1 cut(s) 363
Sfr274I CTCGAG 1 cut(s) 646
SlaI CTCGAG 1 cut(s) 646
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
SnaBI TACGTA 1 cut(s) 392
Sse9I AATT 3 cut(s) 410, 626, 640
SsiI CCGC 1 cut(s) 523
SspMI CTAG 3 cut(s) 18, 155, 504
SstI GAGCTC 1 cut(s) 556
StyD4I CCNGG 3 cut(s) 9, 138, 448
StyI CCWWGG 1 cut(s) 503
TaaI ACNGT 2 cut(s) 32, 175
TaiI ACGT 3 cut(s) 94, 338, 394
TaqI TCGA 3 cut(s) 126, 214, 647
TasI AATT 3 cut(s) 410, 626, 640
TauI GCSGC 1 cut(s) 526
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 3 cut(s) 27, 96, 338
Tru9I TTAA 3 cut(s) 27, 96, 338
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 2 cut(s) 53, 680
VspI ATTAAT 1 cut(s) 96
XhoI CTCGAG 1 cut(s) 646
XmaJI CCTAGG 1 cut(s) 503
XmnI GAANNNNTTC 1 cut(s) 372
XspI CTAG 3 cut(s) 18, 155, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.