Rroxscaffold_5G00353880

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006270
Physical Location & Seq
Reverse (-)
31829648 .. 31832962
3315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00353880.1

Sequence Viewer

Length: 657 bp
ATGGCAAGCCAGGCAGCTAGGTCTTTTAACGGTAACATGAAGAAAGCTGTTGCCGGTCTAAGACGTATTAATCTGGATGGTCTGAGATGGAGAGTATTCGATGCGAAAGGCCAGGTTCTTGGAAGGCTAGCATCTCAAATATCTACTGTAATTCAAGGAAAGGATAAACCAACATACACTCCAAATCGAGATGATGGAGATATGTGCATTGTGCTGAATGCGAAGGATATTTGCGTCACAGGGAGAAAACTGACTGATAAAGTTTATCATTGGCATACTGGGTATGTGGGTCATCTGAAGGAGAGGACGTTAAAAGATCAGATGGTCAAAGATCCTACAGAAGTCATTCGCAAAGCTGTGTTACGCATGCTCCCAAAGAACAAATTGCGCGATGATAGAGATAGAAAGCTGAGGATATTTCCTGGAAGTGAACACCCCTTTGGTGATCGGCCCCTTGAACCATATGTGATGCCCCCTAGGAGAGTAAGGGAAATGCGACCGCGTACAAGACGAGCCATTGTCCGAGCTCAGAAAAAGGCTGAACAGCAACAAGAAGCTGCTAATGATCCACGAAAGGGCAAAAAGAAGGATACACGGGAATTGGTTGCTTCTAATTACTCGAGCTTATTTGGGCTTATGAAAGTTGTTGAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L13

Protein Analysis

218

Amino Acids

25.06

Weight (kDa)

10.34

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L13 PF00572 29 - 146 5e-45 Ribosomal protein L13
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 390, 502
AciI CCGC 1 cut(s) 500
AclWI GGATC 2 cut(s) 326, 560
AcuI CTGAAG 1 cut(s) 317
AfaI GTAC 1 cut(s) 505
AfiI CCNNNNNNNGG 1 cut(s) 575
AgsI TTSAA 2 cut(s) 155, 458
AjnI CCWGG 3 cut(s) 9, 111, 421
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
AluBI AGCT 7 cut(s) 17, 47, 356, 409, 527, 557, 624
AluI AGCT 7 cut(s) 17, 47, 356, 409, 527, 557, 624
Alw21I GWGCWC 1 cut(s) 529
AlwI GGATC 2 cut(s) 326, 560
Ama87I CYCGRG 1 cut(s) 619
AoxI GGCC 2 cut(s) 109, 449
ApeKI GCWGC 2 cut(s) 14, 557
AseI ATTAAT 1 cut(s) 69
Asp700I GAANNNNTTC 1 cut(s) 345
AspA2I CCTAGG 1 cut(s) 476
AspLEI GCGC 1 cut(s) 390
AspS9I GGNCC 1 cut(s) 450
AsuHPI GGTGA 1 cut(s) 455
AsuNHI GCTAGC 1 cut(s) 127
AvaI CYCGRG 1 cut(s) 619
AvrII CCTAGG 1 cut(s) 476
BanII GRGCYC 1 cut(s) 529
Bbv12I GWGCWC 1 cut(s) 529
BbvCI CCTCAGC 1 cut(s) 410
BbvI GCAGC 2 cut(s) 26, 544
BccI CCATC 4 cut(s) 71, 81, 188, 316
BciT130I CCWGG 3 cut(s) 11, 113, 423
BciVI GTATCC 1 cut(s) 583
BfaI CTAG 3 cut(s) 18, 128, 477
BfmI CTRYAG 1 cut(s) 336
BfuI GTATCC 1 cut(s) 583
BisI GCNGC 2 cut(s) 15, 558
BlnI CCTAGG 1 cut(s) 476
BlsI GCNGC 2 cut(s) 16, 559
Bme1390I CCNGG 3 cut(s) 11, 113, 423
BmeT110I CYCGRG 1 cut(s) 619
BmgT120I GGNCC 1 cut(s) 450
BmiI GGNNCC 1 cut(s) 452
BmrFI CCNGG 3 cut(s) 11, 113, 423
BmrI ACTGGG 1 cut(s) 288
BmsI GCATC 3 cut(s) 91, 140, 459
BmtI GCTAGC 1 cut(s) 131
BmuI ACTGGG 1 cut(s) 288
Bpu10I CCTNAGC 1 cut(s) 410
BsaJI CCNNGG 1 cut(s) 476
BsaXI ACNNNNNCTCC 6 cut(s) 163, 193, 293, 323, 354, 384
Bsc4I CCNNNNNNNGG 1 cut(s) 575
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 1 cut(s) 283
BseBI CCWGG 3 cut(s) 11, 113, 423
BseDI CCNNGG 1 cut(s) 476
BseGI GGATG 1 cut(s) 82
BseLI CCNNNNNNNGG 1 cut(s) 575
BseMII CTCAG 3 cut(s) 74, 401, 542
BseNI ACTGG 1 cut(s) 283
BseXI GCAGC 2 cut(s) 26, 544
Bsh1236I CGCG 2 cut(s) 390, 502
Bsh1285I CGRYCG 1 cut(s) 500
BshFI GGCC 2 cut(s) 111, 451
BsiEI CGRYCG 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 529
BsiHKCI CYCGRG 1 cut(s) 619
BsiSI CCGG 1 cut(s) 54
BslI CCNNNNNNNGG 1 cut(s) 575
BsmI GAATGC 1 cut(s) 223
BsnI GGCC 2 cut(s) 111, 451
BsoBI CYCGRG 1 cut(s) 619
Bsp1286I GDGCHC 1 cut(s) 529
Bsp143I GATC 4 cut(s) 316, 331, 445, 565
BspACI CCGC 1 cut(s) 500
BspANI GGCC 2 cut(s) 111, 451
BspCNI CTCAG 3 cut(s) 75, 402, 541
BspFNI CGCG 2 cut(s) 390, 502
BspLI GGNNCC 1 cut(s) 452
BspOI GCTAGC 1 cut(s) 131
BspPI GGATC 2 cut(s) 326, 560
BsrFI RCCGGY 1 cut(s) 53
BsrI ACTGG 1 cut(s) 283
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 476
BssMI GATC 4 cut(s) 316, 331, 445, 565
BssT1I CCWWGG 1 cut(s) 476
Bst2UI CCWGG 3 cut(s) 11, 113, 423
Bst4CI ACNGT 2 cut(s) 32, 148
BstC8I GCNNGC 3 cut(s) 7, 129, 368
BstDEI CTNAG 4 cut(s) 59, 83, 410, 528
BstF5I GGATG 1 cut(s) 82
BstFNI CGCG 2 cut(s) 390, 502
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 4 cut(s) 319, 334, 448, 568
BstMBI GATC 4 cut(s) 316, 331, 445, 565
BstMCI CGRYCG 1 cut(s) 500
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 3 cut(s) 11, 113, 423
BstNSI RCATGY 1 cut(s) 370
BstSCI CCNGG 3 cut(s) 9, 111, 421
BstSFI CTRYAG 1 cut(s) 336
BstUI CGCG 2 cut(s) 390, 502
BstV1I GCAGC 2 cut(s) 26, 544
BstX2I RGATCY 1 cut(s) 331
BstXI CCANNNNNNTGG 1 cut(s) 119
BstYI RGATCY 1 cut(s) 331
BsuI GTATCC 1 cut(s) 583
BsuRI GGCC 2 cut(s) 111, 451
BtgZI GCGATG 1 cut(s) 405
BtsCI GGATG 1 cut(s) 82
Cac8I GCNNGC 3 cut(s) 7, 129, 368
CfoI GCGC 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 450
CseI GACGC 1 cut(s) 223
Csp6I GTAC 1 cut(s) 504
CviAII CATG 2 cut(s) 37, 367
CviQI GTAC 1 cut(s) 504
DdeI CTNAG 4 cut(s) 59, 83, 410, 528
DpnI GATC 4 cut(s) 318, 333, 447, 567
DpnII GATC 4 cut(s) 316, 331, 445, 565
Ecl136II GAGCTC 1 cut(s) 527
Eco130I CCWWGG 1 cut(s) 476
Eco24I GRGCYC 1 cut(s) 529
Eco53kI GAGCTC 1 cut(s) 527
Eco57I CTGAAG 1 cut(s) 317
Eco88I CYCGRG 1 cut(s) 619
EcoICRI GAGCTC 1 cut(s) 527
EcoRII CCWGG 3 cut(s) 9, 111, 421
EcoT14I CCWWGG 1 cut(s) 476
EcoT38I GRGCYC 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 476
FaeI CATG 2 cut(s) 40, 370
FaiI YATR 9 cut(s) 38, 175, 203, 276, 285, 368, 463, 465, 638
FatI CATG 2 cut(s) 36, 366
FauNDI CATATG 1 cut(s) 463
Fnu4HI GCNGC 2 cut(s) 15, 558
FokI GGATG 1 cut(s) 89
FriOI GRGCYC 1 cut(s) 529
Fsp4HI GCNGC 2 cut(s) 15, 558
FspBI CTAG 3 cut(s) 18, 128, 477
GlaI GCGC 1 cut(s) 389
GluI GCNGC 2 cut(s) 15, 558
HaeIII GGCC 2 cut(s) 111, 451
HapII CCGG 1 cut(s) 54
HgaI GACGC 1 cut(s) 223
HhaI GCGC 1 cut(s) 390
Hin1II CATG 2 cut(s) 40, 370
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HpaII CCGG 1 cut(s) 54
HphI GGTGA 1 cut(s) 455
Hpy166II GTNNAC 1 cut(s) 431
Hpy188I TCNGA 5 cut(s) 84, 297, 321, 524, 531
Hpy188III TCNNGA 2 cut(s) 74, 188
Hpy8I GTNNAC 1 cut(s) 431
HpyAV CCTTC 4 cut(s) 117, 217, 292, 580
HpyCH4III ACNGT 2 cut(s) 32, 148
HpyCH4IV ACGT 2 cut(s) 64, 308
HpyCH4V TGCA 1 cut(s) 207
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 4 cut(s) 59, 83, 410, 528
HpySE526I ACGT 2 cut(s) 64, 308
Hsp92II CATG 2 cut(s) 40, 370
HspAI GCGC 1 cut(s) 388
Kzo9I GATC 4 cut(s) 316, 331, 445, 565
LmnI GCTCC 1 cut(s) 375
LpnPI CCDG 9 cut(s) 23, 59, 67, 98, 125, 225, 264, 408, 435
Lsp1109I GCAGC 2 cut(s) 26, 544
LweI GCATC 3 cut(s) 91, 140, 459
MaeI CTAG 3 cut(s) 18, 128, 477
MaeII ACGT 2 cut(s) 64, 308
MaeIII GTNAC 3 cut(s) 32, 235, 360
MalI GATC 4 cut(s) 318, 333, 447, 567
MboI GATC 4 cut(s) 316, 331, 445, 565
MboII GAAGA 1 cut(s) 52
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 1 cut(s) 529
MluCI AATT 4 cut(s) 150, 383, 599, 613
MnlI CCTC 3 cut(s) 297, 405, 643
MroXI GAANNNNTTC 1 cut(s) 345
MseI TTAA 4 cut(s) 27, 69, 311, 655
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 3 cut(s) 11, 113, 423
Mva1269I GAATGC 1 cut(s) 223
MvaI CCWGG 3 cut(s) 11, 113, 423
MvnI CGCG 2 cut(s) 390, 502
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeI CATATG 1 cut(s) 463
NdeII GATC 4 cut(s) 316, 331, 445, 565
NheI GCTAGC 1 cut(s) 127
NlaIII CATG 2 cut(s) 40, 370
NlaIV GGNNCC 1 cut(s) 452
NmuCI GTSAC 1 cut(s) 235
NspI RCATGY 1 cut(s) 370
PaeI GCATGC 1 cut(s) 370
PaeR7I CTCGAG 1 cut(s) 619
PctI GAATGC 1 cut(s) 223
PdmI GAANNNNTTC 1 cut(s) 345
PfoI TCCNGGA 1 cut(s) 421
PkrI GCNGC 2 cut(s) 16, 559
PshBI ATTAAT 1 cut(s) 69
Psp124BI GAGCTC 1 cut(s) 529
Psp6I CCWGG 3 cut(s) 9, 111, 421
PspGI CCWGG 3 cut(s) 9, 111, 421
PspN4I GGNNCC 1 cut(s) 452
PspPI GGNCC 1 cut(s) 450
PspXI VCTCGAGB 1 cut(s) 619
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
SacI GAGCTC 1 cut(s) 529
SaqAI TTAA 4 cut(s) 27, 69, 311, 655
SatI GCNGC 2 cut(s) 15, 558
Sau3AI GATC 4 cut(s) 316, 331, 445, 565
Sau96I GGNCC 1 cut(s) 450
ScrFI CCNGG 3 cut(s) 11, 113, 423
SduI GDGCHC 1 cut(s) 529
SfaNI GCATC 3 cut(s) 91, 140, 459
SfcI CTRYAG 1 cut(s) 336
Sfr274I CTCGAG 1 cut(s) 619
SlaI CTCGAG 1 cut(s) 619
SmlI CTYRAG 1 cut(s) 619
SmoI CTYRAG 1 cut(s) 619
SphI GCATGC 1 cut(s) 370
Sse9I AATT 4 cut(s) 150, 383, 599, 613
SsiI CCGC 1 cut(s) 500
SspMI CTAG 3 cut(s) 18, 128, 477
SstI GAGCTC 1 cut(s) 529
StyD4I CCNGG 3 cut(s) 9, 111, 421
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 2 cut(s) 32, 148
TaiI ACGT 2 cut(s) 67, 311
TaqI TCGA 3 cut(s) 99, 187, 620
TasI AATT 4 cut(s) 150, 383, 599, 613
Tru1I TTAA 4 cut(s) 27, 69, 311, 655
Tru9I TTAA 4 cut(s) 27, 69, 311, 655
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 2 cut(s) 14, 557
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 2 cut(s) 53, 653
VspI ATTAAT 1 cut(s) 69
XceI RCATGY 1 cut(s) 370
XhoI CTCGAG 1 cut(s) 619
XmaJI CCTAGG 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 345
XspI CTAG 3 cut(s) 18, 128, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.