Rh4AG155800

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0006270
Physical Location & Seq
Reverse (-)
39656662 .. 39660518
3857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG155800.1

Sequence Viewer

Length: 492 bp
ATGGCAAGCCAGGCAGCTAGGTCTTTTAACGGTAACATGAAGAAAGCTGTTGCCGGCCTCAGACGTATTAATCTTGATGGTCTGAGATGGAGAGTATTCGATGCGAAAGGCCAGGTTCTTGGAAGGCTAGCATCTCAAATATCTACTGTGATTCAAGGAAAGGATAAACCAACATACACTCCAAATCGAGATGATGGAGATATGTGCATTGTGCTGAACGCGAAGGATATTTGCGTCACGGGTAGTAAACTGACTGATAAAGTTTATCATTGGCATACTGGGTATGTGGGTCATCTGAAGGAGAGGACGTTAAAAGATCAGATGGTCAAAGATCCTGCAGAGGTCATTCACAAAGCTGTGTTACGTATGCTTCCAAAGAACAAATTGCGCGATGTGAGTTTCATTACAGTACCATTTTCTTGTCTGTTGTGTTCTAACACAGAGTATCTTGTATCAAAATATTGTGGAATGCTGCTTCATATTGCAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L13

Protein Analysis

163

Amino Acids

18.22

Weight (kDa)

9.62

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L13 PF00572 29 - 132 7.9e-39 Ribosomal protein L13
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 221, 390
AclWI GGATC 1 cut(s) 326
AcuI CTGAAG 1 cut(s) 317
AfaI GTAC 1 cut(s) 411
AgsI TTSAA 1 cut(s) 155
AjnI CCWGG 2 cut(s) 9, 111
AluBI AGCT 3 cut(s) 17, 47, 356
AluI AGCT 3 cut(s) 17, 47, 356
AlwI GGATC 1 cut(s) 326
AoxI GGCC 2 cut(s) 55, 109
ApeKI GCWGC 2 cut(s) 14, 472
AseI ATTAAT 1 cut(s) 69
AspLEI GCGC 1 cut(s) 390
AsuNHI GCTAGC 1 cut(s) 127
BbvI GCAGC 2 cut(s) 26, 459
BccI CCATC 4 cut(s) 71, 81, 188, 316
BciT130I CCWGG 2 cut(s) 11, 113
BfaI CTAG 2 cut(s) 18, 128
BfmI CTRYAG 1 cut(s) 336
BisI GCNGC 2 cut(s) 15, 473
BlsI GCNGC 2 cut(s) 16, 474
Bme1390I CCNGG 2 cut(s) 11, 113
BmrFI CCNGG 2 cut(s) 11, 113
BmrI ACTGGG 1 cut(s) 288
BmsI GCATC 2 cut(s) 91, 140
BmtI GCTAGC 1 cut(s) 131
BmuI ACTGGG 1 cut(s) 288
BsaAI YACGTR 1 cut(s) 365
BsaXI ACNNNNNCTCC 4 cut(s) 163, 193, 293, 323
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 1 cut(s) 283
BseBI CCWGG 2 cut(s) 11, 113
BseMII CTCAG 2 cut(s) 73, 74
BseNI ACTGG 1 cut(s) 283
BseXI GCAGC 2 cut(s) 26, 459
Bsh1236I CGCG 2 cut(s) 221, 390
BshFI GGCC 2 cut(s) 57, 111
BsiSI CCGG 1 cut(s) 54
BsmI GAATGC 1 cut(s) 474
BsnI GGCC 2 cut(s) 57, 111
Bsp143I GATC 2 cut(s) 316, 331
BspANI GGCC 2 cut(s) 57, 111
BspCNI CTCAG 2 cut(s) 72, 75
BspFNI CGCG 2 cut(s) 221, 390
BspMAI CTGCAG 1 cut(s) 340
BspOI GCTAGC 1 cut(s) 131
BspPI GGATC 1 cut(s) 326
BsrFI RCCGGY 1 cut(s) 53
BsrI ACTGG 1 cut(s) 283
BssAI RCCGGY 1 cut(s) 53
BssMI GATC 2 cut(s) 316, 331
Bst2UI CCWGG 2 cut(s) 11, 113
Bst4CI ACNGT 3 cut(s) 32, 148, 409
BstBAI YACGTR 1 cut(s) 365
BstC8I GCNNGC 3 cut(s) 7, 55, 129
BstDEI CTNAG 2 cut(s) 59, 83
BstFNI CGCG 2 cut(s) 221, 390
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 2 cut(s) 319, 334
BstMBI GATC 2 cut(s) 316, 331
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 11, 113
BstSCI CCNGG 2 cut(s) 9, 111
BstSFI CTRYAG 1 cut(s) 336
BstSNI TACGTA 1 cut(s) 365
BstUI CGCG 2 cut(s) 221, 390
BstV1I GCAGC 2 cut(s) 26, 459
BstX2I RGATCY 1 cut(s) 331
BstXI CCANNNNNNTGG 1 cut(s) 119
BstYI RGATCY 1 cut(s) 331
BsuRI GGCC 2 cut(s) 57, 111
BtgZI GCGATG 1 cut(s) 405
Cac8I GCNNGC 3 cut(s) 7, 55, 129
CfoI GCGC 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 53
CseI GACGC 1 cut(s) 223
Csp6I GTAC 1 cut(s) 410
CviAII CATG 1 cut(s) 37
CviJI RGCY 7 cut(s) 9, 17, 47, 57, 111, 127, 356
CviKI_1 RGCY 7 cut(s) 9, 17, 47, 57, 111, 127, 356
CviQI GTAC 1 cut(s) 410
DdeI CTNAG 2 cut(s) 59, 83
DpnI GATC 2 cut(s) 318, 333
DpnII GATC 2 cut(s) 316, 331
Eco105I TACGTA 1 cut(s) 365
Eco57I CTGAAG 1 cut(s) 317
EcoRII CCWGG 2 cut(s) 9, 111
FaeI CATG 1 cut(s) 40
FaiI YATR 7 cut(s) 38, 175, 203, 276, 285, 368, 480
FatI CATG 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 15, 473
Fsp4HI GCNGC 2 cut(s) 15, 473
FspBI CTAG 2 cut(s) 18, 128
GlaI GCGC 1 cut(s) 389
GluI GCNGC 2 cut(s) 15, 473
HaeIII GGCC 2 cut(s) 57, 111
HapII CCGG 1 cut(s) 54
HgaI GACGC 1 cut(s) 223
HhaI GCGC 1 cut(s) 390
Hin1II CATG 1 cut(s) 40
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HinfI GANTC 1 cut(s) 151
HpaII CCGG 1 cut(s) 54
Hpy166II GTNNAC 1 cut(s) 248
Hpy188I TCNGA 4 cut(s) 62, 84, 297, 321
Hpy188III TCNNGA 2 cut(s) 74, 188
Hpy8I GTNNAC 1 cut(s) 248
HpyAV CCTTC 3 cut(s) 117, 217, 292
HpyCH4III ACNGT 3 cut(s) 32, 148, 409
HpyCH4IV ACGT 3 cut(s) 64, 308, 364
HpyCH4V TGCA 3 cut(s) 207, 338, 485
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 2 cut(s) 59, 83
HpySE526I ACGT 3 cut(s) 64, 308, 364
Hsp92II CATG 1 cut(s) 40
HspAI GCGC 1 cut(s) 388
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 2 cut(s) 316, 331
LpnPI CCDG 7 cut(s) 23, 67, 98, 125, 264, 348, 471
Lsp1109I GCAGC 2 cut(s) 26, 459
LweI GCATC 2 cut(s) 91, 140
MaeI CTAG 2 cut(s) 18, 128
MaeII ACGT 3 cut(s) 64, 308, 364
MaeIII GTNAC 3 cut(s) 32, 235, 360
MalI GATC 2 cut(s) 318, 333
MboI GATC 2 cut(s) 316, 331
MboII GAAGA 1 cut(s) 52
MflI RGATCY 1 cut(s) 331
MluCI AATT 1 cut(s) 383
MnlI CCTC 3 cut(s) 68, 297, 334
MroNI GCCGGC 1 cut(s) 53
MseI TTAA 3 cut(s) 27, 69, 311
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 2 cut(s) 11, 113
Mva1269I GAATGC 1 cut(s) 474
MvaI CCWGG 2 cut(s) 11, 113
MvnI CGCG 2 cut(s) 221, 390
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 55
NdeII GATC 2 cut(s) 316, 331
NgoMIV GCCGGC 1 cut(s) 53
NheI GCTAGC 1 cut(s) 127
NlaIII CATG 1 cut(s) 40
NmuCI GTSAC 1 cut(s) 235
PctI GAATGC 1 cut(s) 474
PdiI GCCGGC 1 cut(s) 55
PfeI GAWTC 1 cut(s) 151
PkrI GCNGC 2 cut(s) 16, 474
Ppu21I YACGTR 1 cut(s) 365
PshBI ATTAAT 1 cut(s) 69
Psp6I CCWGG 2 cut(s) 9, 111
PspGI CCWGG 2 cut(s) 9, 111
PstI CTGCAG 1 cut(s) 340
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 1 cut(s) 411
RsaNI GTAC 1 cut(s) 410
SaqAI TTAA 3 cut(s) 27, 69, 311
SatI GCNGC 2 cut(s) 15, 473
Sau3AI GATC 2 cut(s) 316, 331
ScrFI CCNGG 2 cut(s) 11, 113
SetI ASST 9 cut(s) 19, 23, 49, 67, 117, 311, 345, 358, 367
SfaNI GCATC 2 cut(s) 91, 140
SfcI CTRYAG 1 cut(s) 336
SnaBI TACGTA 1 cut(s) 365
Sse9I AATT 1 cut(s) 383
SspI AATATT 1 cut(s) 461
SspMI CTAG 2 cut(s) 18, 128
StyD4I CCNGG 2 cut(s) 9, 111
TaaI ACNGT 3 cut(s) 32, 148, 409
TaiI ACGT 3 cut(s) 67, 311, 367
TaqI TCGA 2 cut(s) 99, 187
TasI AATT 1 cut(s) 383
TfiI GAWTC 1 cut(s) 151
Tru1I TTAA 3 cut(s) 27, 69, 311
Tru9I TTAA 3 cut(s) 27, 69, 311
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 2 cut(s) 14, 472
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 3 cut(s) 53, 391, 467
VspI ATTAAT 1 cut(s) 69
XspI CTAG 2 cut(s) 18, 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.