Rw2G054130

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006270
Physical Location & Seq
Reverse (-)
81814701 .. 81817081
2381 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G054130.1

Sequence Viewer

Length: 486 bp
ATGGCAACCCAAGCAGCTGCAACTTTTAACGGCAATTTGAAGAAAGCTGTTGCCGGCCTGAGACGGATTAATCTTGATGGGCTGCGGTGGAAAGTGTTTGATGCCAAGGGCCAGGTTCTTGGAAGGCTAGCTTCTCAAATATCTACTGTTATTCAAGGCAAGGATAAACCGACGTATGCTCCAAATCGAGATGATGGAGATATGTGCATTGTGCTGAATGCGAAGGATATTTGTGTCACAGGGAGAAAACTAACCGATAAAGTTTATCATTGGCATACCGGGTATGTTGGGCACCTCAAGGAAAGGACTTTGAAGGATCAGATGGTGAAAGACCCTACAGAAGTCATTCGCAAAGCTGTATTGCGCATGCTTCCTAAGAACAAATTGCGTGACGATAGAGATAGAAAGCTGAGAATTTTTGCTGGCGGTGAACACCCATTTGGTGATAGGCCCCTTGAACCATATCTGATGCCCCCCGAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L13

Protein Analysis

161

Amino Acids

18.24

Weight (kDa)

9.89

Isoelectric Point (pI)

28.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L13 PF00572 29 - 146 1.9e-44 Ribosomal protein L13
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 365
AccB1I GGYRCC 1 cut(s) 291
AciI CCGC 2 cut(s) 85, 426
AclWI GGATC 1 cut(s) 324
AcsI RAATTY 1 cut(s) 414
AgsI TTSAA 4 cut(s) 40, 155, 313, 458
AjnI CCWGG 1 cut(s) 111
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
AluBI AGCT 5 cut(s) 17, 47, 131, 356, 409
AluI AGCT 5 cut(s) 17, 47, 131, 356, 409
Alw26I GTCTC 1 cut(s) 55
AlwI GGATC 1 cut(s) 324
AoxI GGCC 3 cut(s) 55, 109, 449
ApeKI GCWGC 3 cut(s) 14, 17, 82
ApoI RAATTY 1 cut(s) 414
AseI ATTAAT 1 cut(s) 69
Asp700I GAANNNNTTC 1 cut(s) 345
AspLEI GCGC 1 cut(s) 366
AspS9I GGNCC 2 cut(s) 109, 450
AsuC2I CCSGG 1 cut(s) 280
AsuHPI GGTGA 3 cut(s) 337, 440, 455
AsuNHI GCTAGC 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 294
BanI GGYRCC 1 cut(s) 291
BbvI GCAGC 3 cut(s) 4, 26, 69
BccI CCATC 3 cut(s) 71, 188, 316
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 1 cut(s) 113
BcnI CCSGG 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 1 cut(s) 128
BfmI CTRYAG 1 cut(s) 336
BisI GCNGC 3 cut(s) 15, 18, 83
BlsI GCNGC 3 cut(s) 16, 19, 84
Bme1390I CCNGG 2 cut(s) 113, 280
BmgT120I GGNCC 2 cut(s) 109, 450
BmiI GGNNCC 2 cut(s) 293, 452
BmrFI CCNGG 2 cut(s) 113, 280
BmsI GCATC 2 cut(s) 91, 459
BmtI GCTAGC 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 280
BsaJI CCNNGG 1 cut(s) 105
BsaXI ACNNNNNCTCC 2 cut(s) 163, 193
Bse118I RCCGGY 1 cut(s) 53
BseBI CCWGG 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 105
BseMII CTCAG 2 cut(s) 50, 401
BseSI GKGCMC 1 cut(s) 294
BseXI GCAGC 3 cut(s) 4, 26, 69
BshFI GGCC 3 cut(s) 57, 111, 451
BshNI GGYRCC 1 cut(s) 291
BsiSI CCGG 2 cut(s) 54, 279
BsmAI GTCTC 1 cut(s) 55
BsmBI CGTCTC 1 cut(s) 55
BsmI GAATGC 1 cut(s) 223
BsnI GGCC 3 cut(s) 57, 111, 451
Bsp1286I GDGCHC 1 cut(s) 294
Bsp143I GATC 1 cut(s) 316
BspACI CCGC 2 cut(s) 85, 426
BspANI GGCC 3 cut(s) 57, 111, 451
BspCNI CTCAG 2 cut(s) 51, 402
BspLI GGNNCC 2 cut(s) 293, 452
BspOI GCTAGC 1 cut(s) 131
BspPI GGATC 1 cut(s) 324
BspT107I GGYRCC 1 cut(s) 291
BsrFI RCCGGY 1 cut(s) 53
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 105
BssMI GATC 1 cut(s) 316
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 1 cut(s) 113
Bst4CI ACNGT 1 cut(s) 148
BstC8I GCNNGC 4 cut(s) 55, 129, 368, 424
BstDEI CTNAG 3 cut(s) 59, 375, 410
BstHHI GCGC 1 cut(s) 366
BstKTI GATC 1 cut(s) 319
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 1 cut(s) 316
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 113
BstNSI RCATGY 1 cut(s) 370
BstSCI CCNGG 2 cut(s) 111, 278
BstSFI CTRYAG 1 cut(s) 336
BstSLI GKGCMC 1 cut(s) 294
BstV1I GCAGC 3 cut(s) 4, 26, 69
BstXI CCANNNNNNTGG 1 cut(s) 119
BsuRI GGCC 3 cut(s) 57, 111, 451
Cac8I GCNNGC 4 cut(s) 55, 129, 368, 424
CfoI GCGC 1 cut(s) 366
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 2 cut(s) 109, 450
CviAII CATG 1 cut(s) 367
DdeI CTNAG 3 cut(s) 59, 375, 410
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
Eco130I CCWWGG 1 cut(s) 105
EcoO109I RGGNCCY 1 cut(s) 450
EcoRII CCWGG 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 1 cut(s) 370
FaiI YATR 6 cut(s) 177, 203, 276, 285, 368, 463
FalI AAGNNNNNCTT 2 cut(s) 115, 147
FatI CATG 1 cut(s) 366
Fnu4HI GCNGC 3 cut(s) 15, 18, 83
Fsp4HI GCNGC 3 cut(s) 15, 18, 83
FspBI CTAG 1 cut(s) 128
FspI TGCGCA 1 cut(s) 365
GlaI GCGC 1 cut(s) 365
GluI GCNGC 3 cut(s) 15, 18, 83
HaeIII GGCC 3 cut(s) 57, 111, 451
HapII CCGG 2 cut(s) 54, 279
HhaI GCGC 1 cut(s) 366
Hin1II CATG 1 cut(s) 370
Hin6I GCGC 1 cut(s) 364
HinP1I GCGC 1 cut(s) 364
HpaII CCGG 2 cut(s) 54, 279
HphI GGTGA 3 cut(s) 337, 440, 455
Hpy166II GTNNAC 1 cut(s) 431
Hpy188I TCNGA 2 cut(s) 321, 468
Hpy188III TCNNGA 2 cut(s) 74, 188
Hpy8I GTNNAC 1 cut(s) 431
Hpy99I CGWCG 1 cut(s) 175
HpyAV CCTTC 3 cut(s) 117, 217, 307
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 1 cut(s) 173
HpyCH4V TGCA 2 cut(s) 20, 207
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 3 cut(s) 59, 375, 410
HpySE526I ACGT 1 cut(s) 173
Hsp92II CATG 1 cut(s) 370
HspAI GCGC 1 cut(s) 364
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 1 cut(s) 316
LmnI GCTCC 1 cut(s) 184
LpnPI CCDG 7 cut(s) 67, 71, 98, 125, 225, 292, 408
Lsp1109I GCAGC 3 cut(s) 4, 26, 69
LweI GCATC 2 cut(s) 91, 459
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 173
MaeIII GTNAC 2 cut(s) 235, 389
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MboII GAAGA 1 cut(s) 52
MhlI GDGCHC 1 cut(s) 294
MluCI AATT 3 cut(s) 34, 383, 414
MnlI CCTC 1 cut(s) 305
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 345
MseI TTAA 2 cut(s) 27, 69
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 2 cut(s) 54, 279
MspR9I CCNGG 2 cut(s) 113, 280
Mva1269I GAATGC 1 cut(s) 223
MvaI CCWGG 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 1 cut(s) 280
NdeII GATC 1 cut(s) 316
NgoMIV GCCGGC 1 cut(s) 53
NheI GCTAGC 1 cut(s) 127
NlaIII CATG 1 cut(s) 370
NlaIV GGNNCC 2 cut(s) 293, 452
NmuCI GTSAC 2 cut(s) 235, 389
NsbI TGCGCA 1 cut(s) 365
NspI RCATGY 1 cut(s) 370
PaeI GCATGC 1 cut(s) 370
PctI GAATGC 1 cut(s) 223
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 345
PkrI GCNGC 3 cut(s) 16, 19, 84
PshBI ATTAAT 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 111
PspGI CCWGG 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 293, 452
PspPI GGNCC 2 cut(s) 109, 450
PvuII CAGCTG 1 cut(s) 17
SaqAI TTAA 2 cut(s) 27, 69
SatI GCNGC 3 cut(s) 15, 18, 83
Sau3AI GATC 1 cut(s) 316
Sau96I GGNCC 2 cut(s) 109, 450
ScrFI CCNGG 2 cut(s) 113, 280
SduI GDGCHC 1 cut(s) 294
SetI ASST 8 cut(s) 19, 49, 117, 133, 176, 297, 358, 411
SfaNI GCATC 2 cut(s) 91, 459
SfcI CTRYAG 1 cut(s) 336
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SphI GCATGC 1 cut(s) 370
Sse9I AATT 3 cut(s) 34, 383, 414
SsiI CCGC 2 cut(s) 85, 426
SspMI CTAG 1 cut(s) 128
StyD4I CCNGG 2 cut(s) 111, 278
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 1 cut(s) 176
TaqI TCGA 1 cut(s) 187
TasI AATT 3 cut(s) 34, 383, 414
Tru1I TTAA 2 cut(s) 27, 69
Tru9I TTAA 2 cut(s) 27, 69
TseFI GTSAC 2 cut(s) 235, 389
TseI GCWGC 3 cut(s) 14, 17, 82
Tsp45I GTSAC 2 cut(s) 235, 389
TspGWI ACGGA 1 cut(s) 79
VspI ATTAAT 1 cut(s) 69
XapI RAATTY 1 cut(s) 414
XceI RCATGY 1 cut(s) 370
XmnI GAANNNNTTC 1 cut(s) 345
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.