FvH4_7g08340

PGAP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010138
Physical Location & Seq
Forward (+)
8248579 .. 8251699
3121 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g08340.t1

Sequence Viewer

Length: 924 bp
ATGGTGATGGCTATGCTTCTGTCACCATTGAAACCCTTGGCCTGCTCCTCTCCTGCATTTTCTCAAGACCCATCATCGTATAGACCAGCTGTTGTTCTTCCAGGTTTGGGGAACAATTCAGCTGACTACCAGAAGTTGGAGGTGACATTGAGAGGGTATGGAGTGTCCACAGTAACTGCAAAGGTGGCAAGAATTGATTGGCTAAGGAATGCCGCCGGTCTTGTGGATCCGAATTACTGGCGGGGAACTCTCAGTCCTCGACCTGTGCTGGATTGGTATTTGAAGAGGGTGGATGAGGCTGTAAAAGAGGCAAAGGAGCTAGCACAAGGTGGACCTTTATCCCTAGTTGGACATTCAGCAGGAGGATGGCTTGCGCGTCTCTACATGGAAGAATATGGGATATCAGATATTTCCTTGTTGTTGACTCTTGGAACCCCCCACCTACCACCTCCGAGAGGTGTCTCTGGGGTCATTGATCAAACAAGGGGACTCCTGTATTATGTCGAAAAGAAATGCTCAAAAGCTGTTTACACTCCTGAACTGAGATATGTATGTATCGCTGGGAGGTATCTCCAAGGTGCTCGCTTATTTGGCAACTCAAGTGAGAGTGTTGATTCCGCAATTCCAATGGAGAGTTTAGAACCCTCATCAGAGGTTGCTGTGATAAATGATGTGAGCATTTCAACATCAACAACGCCCACTTTGCGTGCACGCTTTGTCGGGCAAGGGTACAAGCAGGTTTGCGGGCAAGCAGATGTTTGGGGTGACGGGGTTGTACCAGAAGTGTCAGCTCATCTGGAGGGTGCACTCAATATTAGCATAGATGGTGTTTACCACTCACCAGTTGGTTCAGATGATATATTGAGACCGTGGTATGGGTCACCTGCGATTGTAGAGCAATGGATACACCACCTCCTAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGAP1

Protein Analysis

308

Amino Acids

33.26

Weight (kDa)

5.89

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_6 PF12697 31 - 151 1e-05 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 892
Acc36I ACCTGC 2 cut(s) 727, 892
AccB7I CCANNNNNTGG 1 cut(s) 136
AccII CGCG 1 cut(s) 376
AciI CCGC 4 cut(s) 213, 241, 618, 744
AclWI GGATC 2 cut(s) 221, 234
AdeI CACNNNGTG 1 cut(s) 329
AfaI GTAC 2 cut(s) 731, 777
AfiI CCNNNNNNNGG 4 cut(s) 107, 136, 455, 875
AgsI TTSAA 3 cut(s) 31, 283, 684
AjnI CCWGG 1 cut(s) 100
AloI GAACNNNNNNTCC 2 cut(s) 238, 270
AluBI AGCT 6 cut(s) 89, 122, 319, 524, 791, 921
AluI AGCT 6 cut(s) 89, 122, 319, 524, 791, 921
Alw21I GWGCWC 3 cut(s) 583, 712, 808
Alw26I GTCTC 3 cut(s) 383, 466, 859
Alw44I GTGCAC 2 cut(s) 708, 804
AlwI GGATC 2 cut(s) 221, 234
AlwNI CAGNNNCTG 1 cut(s) 176
AoxI GGCC 1 cut(s) 39
ApaLI GTGCAC 2 cut(s) 708, 804
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 1 cut(s) 332
AsuHPI GGTGA 6 cut(s) 15, 16, 154, 776, 831, 873
AsuNHI GCTAGC 1 cut(s) 319
AvaII GGWCC 1 cut(s) 332
BaeGI GKGCMC 2 cut(s) 712, 808
BamHI GGATCC 1 cut(s) 226
Bbv12I GWGCWC 3 cut(s) 583, 712, 808
BccI CCATC 3 cut(s) 79, 360, 818
BciT130I CCWGG 1 cut(s) 102
BciVI GTATCC 1 cut(s) 897
BclI TGATCA 1 cut(s) 475
BcoDI GTCTC 3 cut(s) 383, 466, 859
BfaI CTAG 2 cut(s) 320, 344
BfuAI ACCTGC 2 cut(s) 727, 892
BfuI GTATCC 1 cut(s) 897
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 1 cut(s) 102
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 2 cut(s) 228, 433
BmrFI CCNGG 1 cut(s) 102
BmtI GCTAGC 1 cut(s) 323
BpmI CTGGAG 1 cut(s) 818
Bpu10I CCTNAGC 2 cut(s) 203, 917
BpuEI CTTGAG 2 cut(s) 48, 583
BsaI GGTCTC 1 cut(s) 859
BsaJI CCNNGG 3 cut(s) 36, 574, 869
BsaXI ACNNNNNCTCC 1 cut(s) 897
Bsc4I CCNNNNNNNGG 4 cut(s) 107, 136, 455, 875
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 242, 842
Bse3DI GCAATG 1 cut(s) 905
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 3 cut(s) 36, 574, 869
BseGI GGATG 2 cut(s) 298, 371
BseLI CCNNNNNNNGG 4 cut(s) 107, 136, 455, 875
BseMI GCAATG 1 cut(s) 905
BseMII CTCAG 2 cut(s) 265, 533
BseNI ACTGG 2 cut(s) 242, 842
BseRI GAGGAG 1 cut(s) 37
BseSI GKGCMC 2 cut(s) 712, 808
BseYI CCCAGC 1 cut(s) 560
Bsh1236I CGCG 1 cut(s) 376
BshFI GGCC 1 cut(s) 41
BsiHKAI GWGCWC 3 cut(s) 583, 712, 808
BsiSI CCGG 1 cut(s) 216
BslFI GGGAC 1 cut(s) 501
BslI CCNNNNNNNGG 4 cut(s) 107, 136, 455, 875
BsmAI GTCTC 3 cut(s) 383, 466, 859
BsmBI CGTCTC 1 cut(s) 383
BsmFI GGGAC 1 cut(s) 501
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 1 cut(s) 41
Bso31I GGTCTC 1 cut(s) 859
Bsp1286I GDGCHC 3 cut(s) 583, 712, 808
Bsp143I GATC 2 cut(s) 226, 475
BspACI CCGC 4 cut(s) 213, 241, 618, 744
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 2 cut(s) 264, 534
BspFNI CGCG 1 cut(s) 376
BspLI GGNNCC 2 cut(s) 228, 433
BspMI ACCTGC 2 cut(s) 727, 892
BspOI GCTAGC 1 cut(s) 323
BspPI GGATC 2 cut(s) 221, 234
BspTNI GGTCTC 1 cut(s) 859
BsrDI GCAATG 1 cut(s) 905
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 2 cut(s) 242, 842
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 3 cut(s) 36, 574, 869
BssMI GATC 2 cut(s) 226, 475
BssT1I CCWWGG 2 cut(s) 36, 574
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 2 cut(s) 172, 870
Bst6I CTCTTC 1 cut(s) 278
BstC8I GCNNGC 8 cut(s) 43, 321, 372, 583, 708, 712, 746, 750
BstDEI CTNAG 4 cut(s) 203, 251, 542, 917
BstDSI CCRYGG 1 cut(s) 869
BstEII GGTNACC 1 cut(s) 879
BstENI CCTNNNNNAGG 1 cut(s) 453
BstF5I GGATG 2 cut(s) 298, 371
BstFNI CGCG 1 cut(s) 376
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 2 cut(s) 229, 478
BstMAI GTCTC 3 cut(s) 383, 466, 859
BstMBI GATC 2 cut(s) 226, 475
BstMWI GCNNNNNNNGC 3 cut(s) 185, 591, 703
BstNI CCWGG 1 cut(s) 102
BstPI GGTNACC 1 cut(s) 879
BstSCI CCNGG 1 cut(s) 100
BstSLI GKGCMC 2 cut(s) 712, 808
BstUI CGCG 1 cut(s) 376
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BsuI GTATCC 1 cut(s) 897
BsuRI GGCC 1 cut(s) 41
BtgI CCRYGG 1 cut(s) 869
BtsCI GGATG 2 cut(s) 298, 371
BveI ACCTGC 2 cut(s) 727, 892
Cac8I GCNNGC 8 cut(s) 43, 321, 372, 583, 708, 712, 746, 750
CaiI CAGNNNCTG 1 cut(s) 176
CfoI GCGC 1 cut(s) 376
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 332
CseI GACGC 1 cut(s) 365
Csp6I GTAC 2 cut(s) 730, 776
CviAII CATG 1 cut(s) 385
CviQI GTAC 2 cut(s) 730, 776
DdeI CTNAG 4 cut(s) 203, 251, 542, 917
DpnI GATC 2 cut(s) 228, 477
DpnII GATC 2 cut(s) 226, 475
DraIII CACNNNGTG 1 cut(s) 329
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
Eco130I CCWWGG 2 cut(s) 36, 574
Eco31I GGTCTC 1 cut(s) 859
Eco32I GATATC 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 332
Eco91I GGTNACC 1 cut(s) 879
EcoNI CCTNNNNNAGG 1 cut(s) 453
EcoO65I GGTNACC 1 cut(s) 879
EcoRII CCWGG 1 cut(s) 100
EcoRV GATATC 1 cut(s) 402
EcoT14I CCWWGG 2 cut(s) 36, 574
ErhI CCWWGG 2 cut(s) 36, 574
Esp3I CGTCTC 1 cut(s) 383
FaeI CATG 1 cut(s) 388
FaqI GGGAC 1 cut(s) 501
FatI CATG 1 cut(s) 384
FauI CCCGC 2 cut(s) 234, 737
FbaI TGATCA 1 cut(s) 475
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 2 cut(s) 305, 378
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 2 cut(s) 320, 344
GlaI GCGC 1 cut(s) 375
GluI GCNGC 1 cut(s) 213
GsaI CCCAGC 1 cut(s) 564
GsuI CTGGAG 1 cut(s) 818
HaeIII GGCC 1 cut(s) 41
HapII CCGG 1 cut(s) 216
HgaI GACGC 1 cut(s) 365
HhaI GCGC 1 cut(s) 376
Hin1II CATG 1 cut(s) 388
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HincII GTYRAC 1 cut(s) 423
HindII GTYRAC 1 cut(s) 423
HinfI GANTC 3 cut(s) 424, 489, 614
HpaII CCGG 1 cut(s) 216
HphI GGTGA 6 cut(s) 15, 16, 154, 776, 831, 873
Hpy166II GTNNAC 7 cut(s) 168, 332, 423, 529, 710, 806, 832
Hpy188I TCNGA 5 cut(s) 231, 406, 453, 652, 853
Hpy188III TCNNGA 3 cut(s) 65, 536, 797
Hpy8I GTNNAC 7 cut(s) 168, 332, 423, 529, 710, 806, 832
HpyCH4III ACNGT 2 cut(s) 172, 870
HpyCH4V TGCA 4 cut(s) 56, 179, 710, 806
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 591, 703
HpyF3I CTNAG 4 cut(s) 203, 251, 542, 917
Hsp92II CATG 1 cut(s) 388
HspAI GCGC 1 cut(s) 374
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 2 cut(s) 226, 475
LmnI GCTCC 2 cut(s) 50, 316
MaeI CTAG 2 cut(s) 320, 344
MaeIII GTNAC 5 cut(s) 21, 142, 172, 764, 879
MalI GATC 2 cut(s) 228, 477
MboI GATC 2 cut(s) 226, 475
MboII GAAGA 3 cut(s) 89, 295, 401
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 3 cut(s) 583, 712, 808
MluCI AATT 4 cut(s) 115, 192, 232, 621
MlyI GAGTC 2 cut(s) 418, 483
MmeI TCCRAC 2 cut(s) 117, 328
MspA1I CMGCKG 2 cut(s) 89, 122
MspI CCGG 1 cut(s) 216
MspR9I CCNGG 1 cut(s) 102
Mva1269I GAATGC 1 cut(s) 214
MvaI CCWGG 1 cut(s) 102
MvnI CGCG 1 cut(s) 376
MwoI GCNNNNNNNGC 3 cut(s) 185, 591, 703
NdeII GATC 2 cut(s) 226, 475
NheI GCTAGC 1 cut(s) 319
NlaIII CATG 1 cut(s) 388
NlaIV GGNNCC 2 cut(s) 228, 433
NmuCI GTSAC 4 cut(s) 21, 142, 764, 879
PaqCI CACCTGC 1 cut(s) 892
PctI GAATGC 1 cut(s) 214
PfeI GAWTC 1 cut(s) 614
PflMI CCANNNNNTGG 1 cut(s) 136
PkrI GCNGC 1 cut(s) 214
PleI GAGTC 2 cut(s) 418, 483
PpsI GAGTC 2 cut(s) 418, 483
Psp6I CCWGG 1 cut(s) 100
PspEI GGTNACC 1 cut(s) 879
PspFI CCCAGC 1 cut(s) 560
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 228, 433
PspPI GGNCC 1 cut(s) 332
PstNI CAGNNNCTG 1 cut(s) 176
PsuI RGATCY 1 cut(s) 226
PvuII CAGCTG 2 cut(s) 89, 122
RsaI GTAC 2 cut(s) 731, 777
RsaNI GTAC 2 cut(s) 730, 776
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 2 cut(s) 226, 475
Sau96I GGNCC 1 cut(s) 332
SchI GAGTC 2 cut(s) 418, 483
ScrFI CCNGG 1 cut(s) 102
SduI GDGCHC 3 cut(s) 583, 712, 808
SinI GGWCC 1 cut(s) 332
SmlI CTYRAG 2 cut(s) 63, 598
SmoI CTYRAG 2 cut(s) 63, 598
Sse9I AATT 4 cut(s) 115, 192, 232, 621
SsiI CCGC 4 cut(s) 213, 241, 618, 744
SspI AATATT 1 cut(s) 814
SspMI CTAG 2 cut(s) 320, 344
StyD4I CCNGG 1 cut(s) 100
StyI CCWWGG 2 cut(s) 36, 574
TaaI ACNGT 2 cut(s) 172, 870
TaqI TCGA 2 cut(s) 259, 504
TasI AATT 4 cut(s) 115, 192, 232, 621
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 1 cut(s) 614
TseFI GTSAC 4 cut(s) 21, 142, 764, 879
Tsp45I GTSAC 4 cut(s) 21, 142, 764, 879
Van91I CCANNNNNTGG 1 cut(s) 136
VneI GTGCAC 2 cut(s) 708, 804
VpaK11BI GGWCC 1 cut(s) 332
XagI CCTNNNNNAGG 1 cut(s) 453
XcmI CCANNNNNNNNNTGG 1 cut(s) 842
XspI CTAG 2 cut(s) 320, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.