Rroxscaffold_4G00311210

PGAP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010138
Physical Location & Seq
Forward (+)
33510589 .. 33513659
3071 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00311210.1

Sequence Viewer

Length: 777 bp
ATGGTGATGGCTATGCTTCTATCACCATTGAAACCCTTGGCCTACTCCACTTCTGCATTTTCTCAAAACCCATCATCATCATACAAACCAGCTGTTCTTCTTCCAGGTTTGGGGAACAACTCAGCTGACTACAAGAAGTTGGAGATTACATTGAGAGGGTATGGAGTGTCCACAGTGGTTGCTAAGGTGGCAAGAATTGATTGGCTGAGGAATGCCGCCGGTTTGGTGGACCCGAATTACTGGCGGGGAACTCTCAGTCCTCGCCCTGTGCTTAATTGGTATTTGAAGAGGGTGGATGAGGCTGTAAAAGAGGCAAAGGAGCTAGCACAAGGTGGACCTATATCCCTAGTTGGACATTCAGCAGGAGGATGGCTTGCACGTCTCTACATGGAAGAATATGGGAAATCAGATATTTCCCTGTATCTCCAAGGTGCTCGCTTATTTGGCAACTCAAGTGGAAGTGTTGATTCTGGAATTCCAATAGAGAGTTTAGAACCCTCATCAGAGGTTGCTGTTATAAATGGTGTGAGCTCTTCAACATCAACCGCACCCACTTTGCGTGCACGCTTCGTGGGGCAAGGGTACAAGCAGGTTTGTGGGCAGGCAGATGTTTGGGGTGATGGAGTTGTACCAGAAGTATCAGCTCATCTGGAGGGTGCACTCAATATTAGCATAGATGGTGTTTACCACTCACCAGTTGGTTCAGATGATATCTTAAGACAGTGGTATGGGTCTCCCGCAATTGTTGAGCAATGGATACATCACCTCCTGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGAP1

Protein Analysis

258

Amino Acids

27.72

Weight (kDa)

6.21

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_6 PF12697 32 - 144 3.7e-06 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 518
Acc36I ACCTGC 1 cut(s) 580
AciI CCGC 4 cut(s) 216, 244, 546, 738
AcsI RAATTY 1 cut(s) 474
AdeI CACNNNGTG 1 cut(s) 332
AfaI GTAC 2 cut(s) 584, 630
AfiI CCNNNNNNNGG 1 cut(s) 110
AflII CTTAAG 1 cut(s) 715
AgsI TTSAA 3 cut(s) 31, 286, 537
AjiI CACGTC 1 cut(s) 380
AjnI CCWGG 1 cut(s) 103
AloI GAACNNNNNNTCC 2 cut(s) 241, 273
AluBI AGCT 6 cut(s) 92, 125, 322, 531, 644, 774
AluI AGCT 6 cut(s) 92, 125, 322, 531, 644, 774
Alw21I GWGCWC 4 cut(s) 436, 533, 565, 661
Alw26I GTCTC 2 cut(s) 386, 738
Alw44I GTGCAC 2 cut(s) 561, 657
AoxI GGCC 1 cut(s) 39
ApaLI GTGCAC 2 cut(s) 561, 657
ApoI RAATTY 1 cut(s) 474
AspS9I GGNCC 2 cut(s) 229, 335
AsuHPI GGTGA 5 cut(s) 15, 16, 629, 684, 755
AsuNHI GCTAGC 1 cut(s) 322
AvaII GGWCC 2 cut(s) 229, 335
BaeGI GKGCMC 2 cut(s) 565, 661
BanII GRGCYC 1 cut(s) 533
Bbv12I GWGCWC 4 cut(s) 436, 533, 565, 661
BbvCI CCTCAGC 1 cut(s) 206
BccI CCATC 4 cut(s) 79, 363, 614, 671
BciT130I CCWGG 1 cut(s) 105
BciVI GTATCC 1 cut(s) 750
BcoDI GTCTC 2 cut(s) 386, 738
BfaI CTAG 2 cut(s) 323, 347
BfrI CTTAAG 1 cut(s) 715
BfuAI ACCTGC 1 cut(s) 580
BfuI GTATCC 1 cut(s) 750
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 2 cut(s) 229, 335
BmgBI CACGTC 1 cut(s) 380
BmgT120I GGNCC 2 cut(s) 229, 335
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 1 cut(s) 105
BmtI GCTAGC 1 cut(s) 326
BpmI CTGGAG 1 cut(s) 671
Bpu10I CCTNAGC 3 cut(s) 183, 206, 770
BpuEI CTTGAG 1 cut(s) 436
BsaI GGTCTC 1 cut(s) 738
BsaJI CCNNGG 2 cut(s) 36, 427
BsaXI ACNNNNNCTCC 1 cut(s) 750
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 2 cut(s) 245, 695
Bse3DI GCAATG 1 cut(s) 758
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 36, 427
BseGI GGATG 2 cut(s) 301, 374
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMI GCAATG 1 cut(s) 758
BseMII CTCAG 4 cut(s) 135, 197, 268, 761
BseNI ACTGG 2 cut(s) 245, 695
BseSI GKGCMC 2 cut(s) 565, 661
BshFI GGCC 1 cut(s) 41
BsiHKAI GWGCWC 4 cut(s) 436, 533, 565, 661
BsiSI CCGG 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 110
BsmAI GTCTC 2 cut(s) 386, 738
BsmBI CGTCTC 1 cut(s) 386
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 1 cut(s) 41
Bso31I GGTCTC 1 cut(s) 738
Bsp1286I GDGCHC 4 cut(s) 436, 533, 565, 661
BspACI CCGC 4 cut(s) 216, 244, 546, 738
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 4 cut(s) 134, 198, 267, 762
BspLI GGNNCC 1 cut(s) 231
BspMI ACCTGC 1 cut(s) 580
BspOI GCTAGC 1 cut(s) 326
BspQI GCTCTTC 1 cut(s) 538
BspTI CTTAAG 1 cut(s) 715
BspTNI GGTCTC 1 cut(s) 738
BsrDI GCAATG 1 cut(s) 758
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 2 cut(s) 245, 695
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 2 cut(s) 36, 427
BssT1I CCWWGG 2 cut(s) 36, 427
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 2 cut(s) 175, 723
Bst6I CTCTTC 2 cut(s) 281, 538
BstAFI CTTAAG 1 cut(s) 715
BstC8I GCNNGC 6 cut(s) 324, 375, 436, 561, 565, 603
BstDEI CTNAG 5 cut(s) 121, 183, 206, 254, 770
BstF5I GGATG 2 cut(s) 301, 374
BstMAI GTCTC 2 cut(s) 386, 738
BstMWI GCNNNNNNNGC 2 cut(s) 188, 444
BstNI CCWGG 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 103
BstSLI GKGCMC 2 cut(s) 565, 661
BsuI GTATCC 1 cut(s) 750
BsuRI GGCC 1 cut(s) 41
BtrI CACGTC 1 cut(s) 380
BtsCI GGATG 2 cut(s) 301, 374
BtsIMutI CAGTG 2 cut(s) 180, 728
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 6 cut(s) 324, 375, 436, 561, 565, 603
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 229, 335
Csp6I GTAC 2 cut(s) 583, 629
CspCI CAANNNNNGTGG 4 cut(s) 160, 195, 436, 471
CviAII CATG 1 cut(s) 388
CviQI GTAC 2 cut(s) 583, 629
DdeI CTNAG 5 cut(s) 121, 183, 206, 254, 770
DraIII CACNNNGTG 1 cut(s) 332
Eam1104I CTCTTC 2 cut(s) 281, 538
EarI CTCTTC 2 cut(s) 281, 538
Ecl136II GAGCTC 1 cut(s) 531
Eco130I CCWWGG 2 cut(s) 36, 427
Eco24I GRGCYC 1 cut(s) 533
Eco31I GGTCTC 1 cut(s) 738
Eco32I GATATC 1 cut(s) 712
Eco47I GGWCC 2 cut(s) 229, 335
Eco53kI GAGCTC 1 cut(s) 531
EcoICRI GAGCTC 1 cut(s) 531
EcoRI GAATTC 1 cut(s) 474
EcoRII CCWGG 1 cut(s) 103
EcoRV GATATC 1 cut(s) 712
EcoT14I CCWWGG 2 cut(s) 36, 427
EcoT38I GRGCYC 1 cut(s) 533
ErhI CCWWGG 2 cut(s) 36, 427
Esp3I CGTCTC 1 cut(s) 386
FaeI CATG 1 cut(s) 391
FaiI YATR 9 cut(s) 14, 82, 162, 341, 389, 399, 518, 674, 729
FatI CATG 1 cut(s) 387
FauI CCCGC 2 cut(s) 237, 745
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 308, 381
FriOI GRGCYC 1 cut(s) 533
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 2 cut(s) 323, 347
GluI GCNGC 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 671
HaeIII GGCC 1 cut(s) 41
HapII CCGG 1 cut(s) 219
Hin1II CATG 1 cut(s) 391
HinfI GANTC 1 cut(s) 467
HpaII CCGG 1 cut(s) 219
HphI GGTGA 5 cut(s) 15, 16, 629, 684, 755
Hpy166II GTNNAC 6 cut(s) 171, 229, 335, 563, 659, 685
Hpy188I TCNGA 3 cut(s) 409, 505, 706
Hpy188III TCNNGA 3 cut(s) 471, 650, 769
Hpy8I GTNNAC 6 cut(s) 171, 229, 335, 563, 659, 685
HpyCH4III ACNGT 2 cut(s) 175, 723
HpyCH4IV ACGT 1 cut(s) 379
HpyCH4V TGCA 4 cut(s) 56, 377, 563, 659
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 444
HpyF3I CTNAG 5 cut(s) 121, 183, 206, 254, 770
HpySE526I ACGT 1 cut(s) 379
Hsp92II CATG 1 cut(s) 391
LguI GCTCTTC 1 cut(s) 538
LmnI GCTCC 1 cut(s) 319
MaeI CTAG 2 cut(s) 323, 347
MaeII ACGT 1 cut(s) 379
MboII GAAGA 5 cut(s) 89, 92, 298, 404, 525
MfeI CAATTG 1 cut(s) 741
MhlI GDGCHC 4 cut(s) 436, 533, 565, 661
MluCI AATT 5 cut(s) 195, 235, 274, 474, 741
MmeI TCCRAC 2 cut(s) 120, 331
MseI TTAA 2 cut(s) 273, 716
MspA1I CMGCKG 2 cut(s) 92, 125
MspCI CTTAAG 1 cut(s) 715
MspI CCGG 1 cut(s) 219
MspR9I CCNGG 1 cut(s) 105
MunI CAATTG 1 cut(s) 741
Mva1269I GAATGC 1 cut(s) 217
MvaI CCWGG 1 cut(s) 105
MwoI GCNNNNNNNGC 2 cut(s) 188, 444
NheI GCTAGC 1 cut(s) 322
NlaIII CATG 1 cut(s) 391
NlaIV GGNNCC 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 538
PctI GAATGC 1 cut(s) 217
PfeI GAWTC 1 cut(s) 467
PkrI GCNGC 1 cut(s) 217
PsiI TTATAA 1 cut(s) 518
Psp124BI GAGCTC 1 cut(s) 533
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 231
PspPI GGNCC 2 cut(s) 229, 335
PvuII CAGCTG 2 cut(s) 92, 125
RsaI GTAC 2 cut(s) 584, 630
RsaNI GTAC 2 cut(s) 583, 629
SacI GAGCTC 1 cut(s) 533
SapI GCTCTTC 1 cut(s) 538
SaqAI TTAA 2 cut(s) 273, 716
SatI GCNGC 1 cut(s) 216
Sau96I GGNCC 2 cut(s) 229, 335
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 4 cut(s) 436, 533, 565, 661
SinI GGWCC 2 cut(s) 229, 335
SmlI CTYRAG 2 cut(s) 451, 715
SmoI CTYRAG 2 cut(s) 451, 715
Sse9I AATT 5 cut(s) 195, 235, 274, 474, 741
SsiI CCGC 4 cut(s) 216, 244, 546, 738
SspI AATATT 1 cut(s) 667
SspMI CTAG 2 cut(s) 323, 347
SstI GAGCTC 1 cut(s) 533
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 2 cut(s) 36, 427
TaaI ACNGT 2 cut(s) 175, 723
TaiI ACGT 1 cut(s) 382
TasI AATT 5 cut(s) 195, 235, 274, 474, 741
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 2 cut(s) 273, 716
Tru9I TTAA 2 cut(s) 273, 716
TscAI CASTG 2 cut(s) 180, 728
TspRI CASTG 2 cut(s) 180, 728
Vha464I CTTAAG 1 cut(s) 715
VneI GTGCAC 2 cut(s) 561, 657
VpaK11BI GGWCC 2 cut(s) 229, 335
XapI RAATTY 1 cut(s) 474
XcmI CCANNNNNNNNNTGG 1 cut(s) 695
XspI CTAG 2 cut(s) 323, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.