FvH4_7g08910

ATP synthase subunit delta'

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003919
Physical Location & Seq
Forward (+)
8702501 .. 8706484
3984 bp
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UTR
Exon/CDS
Intron
FvH4_7g08910.t1

Sequence Viewer

Length: 615 bp
ATGCTGAGACGCGCCACCGCCCTCTTGACCCGACCCGCATCCTCCTCACTCCGGGCCGCCCGTGCCTTCTCCACCGAGGTCGCCGAAGCAGGCGACGCTCCGGCTAACCCCGCTTTCAACGAGGCCTGGAAGAAGGTCGTCCCTCACATCGAGCCCCCCAAGACTCCTCTCTCCTTCATGAAGCCCCGCCCCGCCGTCCCGTCGTCGATCCCGACTAAGCTCACCGTCAACTTCGTCCTGCCTTACGCATCTGAGCTCTCCGCTAAAGAGGTGGACATGGTTATAATTCCAGCAAGCACTGGGCAGATGGGTGTTCTTCCTGGACATGTGGCAACAATTGCCGAGTTGAAACCTGGGATCCTATCAGTACATGAAGGAAATGATGTGACAAAGTACTTCGTCAGCAGTGGTTTTGCATTCATCCATGCGAACTCTTACGCAGATATAATTGCTGTTGAGGCTGTACCTATCGATCGAGTCGATGCAAGTCTTGTCCAGAAGGGGCTTGCAGAGTTCACCCAGAAGCTGAACTCGGCCTCAACTGACTTGGAGAAAGCTGAAGCCCAGATTGGAGTTGATGTGCATAGTGCCCTCAATGCTGCTCTCACAGGCTAG

Protein Analysis

205

Amino Acids

21.53

Weight (kDa)

6.2

Isoelectric Point (pI)

33.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_DE_N PF02823 74 - 145 5.9e-16 ATP synthase, Delta/Epsilon chain, beta-sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 284
AccII CGCG 1 cut(s) 12
AciI CCGC 7 cut(s) 18, 36, 57, 111, 187, 192, 261
AclWI GGATC 3 cut(s) 202, 352, 365
AcuI CTGAAG 1 cut(s) 579
AfaI GTAC 3 cut(s) 369, 395, 465
AfiI CCNNNNNNNGG 1 cut(s) 51
AflIII ACRYGT 1 cut(s) 325
AgsI TTSAA 2 cut(s) 118, 349
AjnI CCWGG 3 cut(s) 125, 319, 352
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AluBI AGCT 4 cut(s) 220, 256, 526, 557
AluI AGCT 4 cut(s) 220, 256, 526, 557
Alw21I GWGCWC 1 cut(s) 258
AlwI GGATC 3 cut(s) 202, 352, 365
AlwNI CAGNNNCTG 1 cut(s) 526
AoxI GGCC 3 cut(s) 54, 123, 534
ApeKI GCWGC 1 cut(s) 599
AspLEI GCGC 1 cut(s) 14
AspS9I GGNCC 1 cut(s) 54
AsuC2I CCSGG 1 cut(s) 53
AsuHPI GGTGA 2 cut(s) 214, 508
BaeGI GKGCMC 1 cut(s) 592
BamHI GGATCC 1 cut(s) 357
BanII GRGCYC 2 cut(s) 156, 258
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 1 cut(s) 586
BccI CCATC 1 cut(s) 301
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 3 cut(s) 127, 321, 354
BcnI CCSGG 1 cut(s) 53
BfaI CTAG 1 cut(s) 613
BisI GCNGC 2 cut(s) 57, 600
BlsI GCNGC 2 cut(s) 58, 601
BmcAI AGTACT 1 cut(s) 395
Bme1390I CCNGG 4 cut(s) 53, 127, 321, 354
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 1 cut(s) 359
BmrFI CCNGG 4 cut(s) 53, 127, 321, 354
BmrI ACTGGG 1 cut(s) 309
BmsI GCATC 3 cut(s) 47, 257, 472
BmuI ACTGGG 1 cut(s) 309
BpuMI CCSGG 1 cut(s) 53
Bsa29I ATCGAT 1 cut(s) 471
BsaJI CCNNGG 2 cut(s) 75, 353
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bsc4I CCNNNNNNNGG 1 cut(s) 51
Bse1I ACTGG 1 cut(s) 304
BseBI CCWGG 3 cut(s) 127, 321, 354
BseCI ATCGAT 1 cut(s) 471
BseDI CCNNGG 2 cut(s) 75, 353
BseGI GGATG 2 cut(s) 38, 420
BseLI CCNNNNNNNGG 1 cut(s) 51
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 1 cut(s) 304
BseRI GAGGAG 2 cut(s) 34, 156
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 1 cut(s) 586
Bsh1236I CGCG 1 cut(s) 12
Bsh1285I CGRYCG 1 cut(s) 475
BshFI GGCC 3 cut(s) 56, 125, 536
BshVI ATCGAT 1 cut(s) 471
BsiEI CGRYCG 1 cut(s) 475
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 2 cut(s) 52, 101
BslFI GGGAC 2 cut(s) 125, 182
BslI CCNNNNNNNGG 1 cut(s) 51
BsmFI GGGAC 2 cut(s) 125, 182
BsmI GAATGC 1 cut(s) 416
BsnI GGCC 3 cut(s) 56, 125, 536
Bsp1286I GDGCHC 3 cut(s) 156, 258, 592
Bsp143I GATC 3 cut(s) 207, 357, 472
BspACI CCGC 7 cut(s) 18, 36, 57, 111, 187, 192, 261
BspANI GGCC 3 cut(s) 56, 125, 536
BspCNI CTCAG 1 cut(s) 244
BspDI ATCGAT 1 cut(s) 471
BspFNI CGCG 1 cut(s) 12
BspHI TCATGA 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 359
BspPI GGATC 3 cut(s) 202, 352, 365
BsrI ACTGG 1 cut(s) 304
BssECI CCNNGG 2 cut(s) 75, 353
BssMI GATC 3 cut(s) 207, 357, 472
Bst2UI CCWGG 3 cut(s) 127, 321, 354
Bst4CI ACNGT 1 cut(s) 226
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 3 cut(s) 91, 295, 507
BstDEI CTNAG 3 cut(s) 5, 216, 252
BstF5I GGATG 2 cut(s) 38, 420
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 3 cut(s) 210, 360, 475
BstMBI GATC 3 cut(s) 207, 357, 472
BstMCI CGRYCG 1 cut(s) 475
BstMWI GCNNNNNNNGC 6 cut(s) 62, 95, 110, 338, 458, 596
BstNI CCWGG 3 cut(s) 127, 321, 354
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 4 cut(s) 51, 125, 319, 352
BstSLI GKGCMC 1 cut(s) 592
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 1 cut(s) 586
BstX2I RGATCY 1 cut(s) 357
BstYI RGATCY 1 cut(s) 357
Bsu15I ATCGAT 1 cut(s) 471
BsuRI GGCC 3 cut(s) 56, 125, 536
BsuTUI ATCGAT 1 cut(s) 471
BtsCI GGATG 2 cut(s) 38, 420
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 2 cut(s) 297, 412
Cac8I GCNNGC 3 cut(s) 91, 295, 507
CaiI CAGNNNCTG 1 cut(s) 526
CciI TCATGA 1 cut(s) 177
CfoI GCGC 1 cut(s) 14
Cfr13I GGNCC 1 cut(s) 54
ClaI ATCGAT 1 cut(s) 471
CseI GACGC 2 cut(s) 18, 104
Csp6I GTAC 3 cut(s) 368, 394, 464
CviAII CATG 5 cut(s) 178, 277, 326, 371, 425
CviQI GTAC 3 cut(s) 368, 394, 464
DdeI CTNAG 3 cut(s) 5, 216, 252
DpnI GATC 3 cut(s) 209, 359, 474
DpnII GATC 3 cut(s) 207, 357, 472
Ecl136II GAGCTC 1 cut(s) 256
Eco147I AGGCCT 1 cut(s) 125
Eco24I GRGCYC 2 cut(s) 156, 258
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 579
EcoICRI GAGCTC 1 cut(s) 256
EcoRII CCWGG 3 cut(s) 125, 319, 352
EcoT38I GRGCYC 2 cut(s) 156, 258
FaeI CATG 5 cut(s) 181, 280, 329, 374, 428
FaiI YATR 8 cut(s) 179, 278, 284, 327, 372, 426, 446, 585
FaqI GGGAC 2 cut(s) 125, 182
FatI CATG 5 cut(s) 177, 276, 325, 370, 424
FauI CCCGC 4 cut(s) 43, 118, 194, 199
Fnu4HI GCNGC 2 cut(s) 57, 600
FokI GGATG 2 cut(s) 25, 407
FriOI GRGCYC 2 cut(s) 156, 258
Fsp4HI GCNGC 2 cut(s) 57, 600
FspBI CTAG 1 cut(s) 613
GlaI GCGC 1 cut(s) 13
GluI GCNGC 2 cut(s) 57, 600
HaeIII GGCC 3 cut(s) 56, 125, 536
HapII CCGG 2 cut(s) 52, 101
HgaI GACGC 2 cut(s) 18, 104
HhaI GCGC 1 cut(s) 14
Hin1II CATG 5 cut(s) 181, 280, 329, 374, 428
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 2 cut(s) 163, 477
HpaII CCGG 2 cut(s) 52, 101
HphI GGTGA 2 cut(s) 214, 508
Hpy166II GTNNAC 3 cut(s) 229, 274, 516
Hpy188I TCNGA 1 cut(s) 253
Hpy188III TCNNGA 4 cut(s) 25, 178, 211, 496
Hpy8I GTNNAC 3 cut(s) 229, 274, 516
Hpy99I CGWCG 3 cut(s) 98, 205, 208
HpyAV CCTTC 5 cut(s) 76, 127, 184, 368, 493
HpyCH4III ACNGT 1 cut(s) 226
HpyCH4V TGCA 4 cut(s) 416, 485, 509, 583
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 95, 110, 338, 458, 596
HpyF3I CTNAG 3 cut(s) 5, 216, 252
Hsp92II CATG 5 cut(s) 181, 280, 329, 374, 428
HspAI GCGC 1 cut(s) 12
Kzo9I GATC 3 cut(s) 207, 357, 472
LmnI GCTCC 1 cut(s) 103
Lsp1109I GCAGC 1 cut(s) 586
LweI GCATC 3 cut(s) 47, 257, 472
MaeI CTAG 1 cut(s) 613
MaeIII GTNAC 1 cut(s) 385
MalI GATC 3 cut(s) 209, 359, 474
MboI GATC 3 cut(s) 207, 357, 472
MboII GAAGA 2 cut(s) 142, 308
MfeI CAATTG 1 cut(s) 336
MflI RGATCY 1 cut(s) 357
MhlI GDGCHC 3 cut(s) 156, 258, 592
MluCI AATT 3 cut(s) 285, 336, 447
MlyI GAGTC 2 cut(s) 157, 486
MspI CCGG 2 cut(s) 52, 101
MspR9I CCNGG 4 cut(s) 53, 127, 321, 354
MunI CAATTG 1 cut(s) 336
Mva1269I GAATGC 1 cut(s) 416
MvaI CCWGG 3 cut(s) 127, 321, 354
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 6 cut(s) 62, 95, 110, 338, 458, 596
NciI CCSGG 1 cut(s) 53
NdeII GATC 3 cut(s) 207, 357, 472
NlaIII CATG 5 cut(s) 181, 280, 329, 374, 428
NlaIV GGNNCC 1 cut(s) 359
NmeAIII GCCGAG 2 cut(s) 367, 512
NmuCI GTSAC 1 cut(s) 385
NspI RCATGY 1 cut(s) 329
PagI TCATGA 1 cut(s) 177
PceI AGGCCT 1 cut(s) 125
PciI ACATGT 1 cut(s) 325
PcsI WCGNNNNNNNCGW 2 cut(s) 209, 477
PctI GAATGC 1 cut(s) 416
PfoI TCCNGGA 1 cut(s) 319
PkrI GCNGC 2 cut(s) 58, 601
Ple19I CGATCG 1 cut(s) 475
PleI GAGTC 2 cut(s) 157, 485
PpsI GAGTC 2 cut(s) 157, 485
PscI ACATGT 1 cut(s) 325
PsiI TTATAA 1 cut(s) 284
Psp124BI GAGCTC 1 cut(s) 258
Psp6I CCWGG 3 cut(s) 125, 319, 352
PspGI CCWGG 3 cut(s) 125, 319, 352
PspN4I GGNNCC 1 cut(s) 359
PspPI GGNCC 1 cut(s) 54
PstNI CAGNNNCTG 1 cut(s) 526
PsuI RGATCY 1 cut(s) 357
PvuI CGATCG 1 cut(s) 475
RsaI GTAC 3 cut(s) 369, 395, 465
RsaNI GTAC 3 cut(s) 368, 394, 464
SacI GAGCTC 1 cut(s) 258
SatI GCNGC 2 cut(s) 57, 600
Sau3AI GATC 3 cut(s) 207, 357, 472
Sau96I GGNCC 1 cut(s) 54
ScaI AGTACT 1 cut(s) 395
SchI GAGTC 2 cut(s) 157, 486
ScrFI CCNGG 4 cut(s) 53, 127, 321, 354
SduI GDGCHC 3 cut(s) 156, 258, 592
SetI ASST 9 cut(s) 81, 138, 222, 258, 273, 355, 469, 528, 559
SfaNI GCATC 3 cut(s) 47, 257, 472
Sse9I AATT 3 cut(s) 285, 336, 447
SseBI AGGCCT 1 cut(s) 125
SsiI CCGC 7 cut(s) 18, 36, 57, 111, 187, 192, 261
SspMI CTAG 1 cut(s) 613
SstI GAGCTC 1 cut(s) 258
StuI AGGCCT 1 cut(s) 125
StyD4I CCNGG 4 cut(s) 51, 125, 319, 352
TaaI ACNGT 1 cut(s) 226
TaqI TCGA 5 cut(s) 150, 206, 471, 475, 480
TasI AATT 3 cut(s) 285, 336, 447
TatI WGTACW 2 cut(s) 367, 393
TauI GCSGC 1 cut(s) 59
TscAI CASTG 2 cut(s) 304, 412
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 1 cut(s) 599
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 4 cut(s) 166, 194, 387, 409
TspRI CASTG 2 cut(s) 304, 412
XceI RCATGY 1 cut(s) 329
XspI CTAG 1 cut(s) 613
ZrmI AGTACT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.