pycom02g18930

ATP synthase subunit delta'

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003919
Physical Location & Seq
Reverse (-)
16980911 .. 16983439
2529 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g18930.1

Sequence Viewer

Length: 603 bp
ATGTTCCGCCGAGCCACCACCCTCTTGACCAGATCCACGATTCCCCTCAGGGCCCGGCCCTTCTCCACCGACGTCGCTGAGGCTGCCCCAGCGGATTCGACTTTCACTGACGCCTGGAAGAAAGTGGTCCCGCACATAGACCCCCCCAAGACCCCATTGACCTTCATGAAGCCTCGGCCTGCTACCCCGTCGTCGATCCCCACTAAGCTCACTGTCAACTTTGTGCTCCCTTATGCGTCCGAGCTTTCGGCTAAAGAGGTTGACATGGTAATAATACCAGCGAGCACTGGGCATATGGGTGTTCTTCCTGGACATGTAGCAACAATTGCAGAGTTGAAACCTGGGGTCATGTCGGTACATGAAGGAACTGATGTCACAAAGTATTTTGTCAGCAGTGGTTTTGCATTCGTCCACGCCAACTCTATTGCAGACATAATTGCTGTGGAGGCAGTCCCACTTGACCGTGTCGATGCAAGTCTTGTCCAGAAGGGGCTTGCAGAGTTCAACCAGAAGCTGAACTCAGCGTCGACTGACTTGGAGAAAGCTGAAGCCCAGATTGGAGTCGATGTGCATAGTGCCCTCAACTCTGCGCTCACCGGCTAG

Protein Analysis

201

Amino Acids

21.29

Weight (kDa)

6.29

Isoelectric Point (pI)

29.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_DE_N PF02823 70 - 142 4.7e-16 ATP synthase, Delta/Epsilon chain, beta-sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 75
AccI GTMKAC 1 cut(s) 527
AciI CCGC 3 cut(s) 7, 92, 131
AclWI GGATC 2 cut(s) 27, 190
AcuI CTGAAG 1 cut(s) 567
AcyI GRCGYC 2 cut(s) 72, 111
AfaI GTAC 1 cut(s) 357
AflIII ACRYGT 1 cut(s) 313
AgsI TTSAA 2 cut(s) 337, 505
AjnI CCWGG 3 cut(s) 113, 307, 340
AjuI GAANNNNNNNTTGG 2 cut(s) 540, 572
AluBI AGCT 4 cut(s) 208, 244, 514, 545
AluI AGCT 4 cut(s) 208, 244, 514, 545
Alw21I GWGCWC 2 cut(s) 228, 287
AlwI GGATC 2 cut(s) 27, 190
AlwNI CAGNNNCTG 1 cut(s) 514
AoxI GGCC 3 cut(s) 51, 56, 176
ApaI GGGCCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 83
AspLEI GCGC 1 cut(s) 592
AspS9I GGNCC 4 cut(s) 51, 52, 57, 127
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 1 cut(s) 586
AvaII GGWCC 1 cut(s) 127
AxyI CCTNAGG 1 cut(s) 47
BaeGI GKGCMC 2 cut(s) 55, 580
BanII GRGCYC 1 cut(s) 55
Bbv12I GWGCWC 2 cut(s) 228, 287
BbvCI CCTCAGC 1 cut(s) 78
BbvI GCAGC 1 cut(s) 70
BciT130I CCWGG 3 cut(s) 115, 309, 342
BcnI CCSGG 1 cut(s) 55
BfaI CTAG 1 cut(s) 601
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 4 cut(s) 55, 115, 309, 342
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 4 cut(s) 51, 52, 57, 127
BmiI GGNNCC 2 cut(s) 53, 129
BmrFI CCNGG 4 cut(s) 55, 115, 309, 342
BmrI ACTGGG 1 cut(s) 297
BmsI GCATC 1 cut(s) 460
BmuI ACTGGG 1 cut(s) 297
Bpu10I CCTNAGC 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 55
BsaHI GRCGYC 2 cut(s) 72, 111
BsaJI CCNNGG 2 cut(s) 173, 341
BsaXI ACNNNNNCTCC 2 cut(s) 552, 582
Bse118I RCCGGY 1 cut(s) 596
Bse1I ACTGG 1 cut(s) 292
Bse21I CCTNAGG 1 cut(s) 47
BseBI CCWGG 3 cut(s) 115, 309, 342
BseDI CCNNGG 2 cut(s) 173, 341
BseMII CTCAG 3 cut(s) 61, 69, 534
BseNI ACTGG 1 cut(s) 292
BseSI GKGCMC 2 cut(s) 55, 580
BseXI GCAGC 1 cut(s) 70
BseYI CCCAGC 1 cut(s) 88
BshFI GGCC 3 cut(s) 53, 58, 178
BsiHKAI GWGCWC 2 cut(s) 228, 287
BsiSI CCGG 2 cut(s) 55, 597
BslFI GGGAC 2 cut(s) 113, 437
BsmFI GGGAC 2 cut(s) 113, 437
BsmI GAATGC 1 cut(s) 404
BsnI GGCC 3 cut(s) 53, 58, 178
Bsp120I GGGCCC 1 cut(s) 51
Bsp1286I GDGCHC 4 cut(s) 55, 228, 287, 580
Bsp143I GATC 2 cut(s) 32, 195
BspACI CCGC 3 cut(s) 7, 92, 131
BspANI GGCC 3 cut(s) 53, 58, 178
BspCNI CTCAG 3 cut(s) 60, 70, 533
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 2 cut(s) 53, 129
BspPI GGATC 2 cut(s) 27, 190
BsrFI RCCGGY 1 cut(s) 596
BsrI ACTGG 1 cut(s) 292
BssAI RCCGGY 1 cut(s) 596
BssECI CCNNGG 2 cut(s) 173, 341
BssMI GATC 2 cut(s) 32, 195
BssNI GRCGYC 2 cut(s) 72, 111
Bst2UI CCWGG 3 cut(s) 115, 309, 342
Bst4CI ACNGT 2 cut(s) 214, 464
BstACI GRCGYC 2 cut(s) 72, 111
BstAPI GCANNNNNTGC 1 cut(s) 326
BstC8I GCNNGC 3 cut(s) 180, 283, 495
BstDEI CTNAG 4 cut(s) 47, 78, 204, 520
BstHHI GCGC 1 cut(s) 592
BstKTI GATC 2 cut(s) 35, 198
BstMBI GATC 2 cut(s) 32, 195
BstMWI GCNNNNNNNGC 4 cut(s) 83, 89, 326, 446
BstNI CCWGG 3 cut(s) 115, 309, 342
BstNSI RCATGY 1 cut(s) 317
BstSCI CCNGG 4 cut(s) 53, 113, 307, 340
BstSLI GKGCMC 2 cut(s) 55, 580
BstV1I GCAGC 1 cut(s) 70
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
Bsu36I CCTNAGG 1 cut(s) 47
BsuRI GGCC 3 cut(s) 53, 58, 178
BtsI GCAGTG 1 cut(s) 400
BtsIMutI CAGTG 4 cut(s) 105, 210, 285, 400
Cac8I GCNNGC 3 cut(s) 180, 283, 495
CaiI CAGNNNCTG 1 cut(s) 514
CciI TCATGA 1 cut(s) 165
CfoI GCGC 1 cut(s) 592
Cfr10I RCCGGY 1 cut(s) 596
Cfr13I GGNCC 4 cut(s) 51, 52, 57, 127
CseI GACGC 3 cut(s) 119, 225, 513
Csp6I GTAC 1 cut(s) 356
CviAII CATG 5 cut(s) 166, 265, 314, 349, 359
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 4 cut(s) 47, 78, 204, 520
DpnI GATC 2 cut(s) 34, 197
DpnII GATC 2 cut(s) 32, 195
Eco24I GRGCYC 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 567
Eco81I CCTNAGG 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 51
EcoRII CCWGG 3 cut(s) 113, 307, 340
EcoT38I GRGCYC 1 cut(s) 55
FaeI CATG 5 cut(s) 169, 268, 317, 352, 362
FaqI GGGAC 2 cut(s) 113, 437
FatI CATG 5 cut(s) 165, 264, 313, 348, 358
FauI CCCGC 1 cut(s) 138
FauNDI CATATG 1 cut(s) 294
FblI GTMKAC 1 cut(s) 527
Fnu4HI GCNGC 1 cut(s) 84
FriOI GRGCYC 1 cut(s) 55
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 1 cut(s) 601
GlaI GCGC 1 cut(s) 591
GluI GCNGC 1 cut(s) 84
GsaI CCCAGC 1 cut(s) 92
HaeIII GGCC 3 cut(s) 53, 58, 178
HapII CCGG 2 cut(s) 55, 597
HgaI GACGC 3 cut(s) 119, 225, 513
HhaI GCGC 1 cut(s) 592
Hin1I GRCGYC 2 cut(s) 72, 111
Hin1II CATG 5 cut(s) 169, 268, 317, 352, 362
Hin6I GCGC 1 cut(s) 590
HinP1I GCGC 1 cut(s) 590
HincII GTYRAC 3 cut(s) 217, 262, 528
HindII GTYRAC 3 cut(s) 217, 262, 528
HinfI GANTC 3 cut(s) 40, 95, 561
HpaII CCGG 2 cut(s) 55, 597
HphI GGTGA 1 cut(s) 586
Hpy166II GTNNAC 4 cut(s) 217, 262, 412, 528
Hpy188I TCNGA 1 cut(s) 241
Hpy188III TCNNGA 3 cut(s) 25, 166, 484
Hpy8I GTNNAC 4 cut(s) 217, 262, 412, 528
Hpy99I CGWCG 5 cut(s) 74, 77, 193, 196, 529
HpyAV CCTTC 4 cut(s) 70, 172, 356, 481
HpyCH4III ACNGT 2 cut(s) 214, 464
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 6 cut(s) 329, 404, 428, 473, 497, 571
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 89, 326, 446
HpyF3I CTNAG 4 cut(s) 47, 78, 204, 520
HpySE526I ACGT 1 cut(s) 72
Hsp92I GRCGYC 2 cut(s) 72, 111
Hsp92II CATG 5 cut(s) 169, 268, 317, 352, 362
HspAI GCGC 1 cut(s) 590
Kzo9I GATC 2 cut(s) 32, 195
LmnI GCTCC 1 cut(s) 231
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 1 cut(s) 460
MaeI CTAG 1 cut(s) 601
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 373
MalI GATC 2 cut(s) 34, 197
MboI GATC 2 cut(s) 32, 195
MboII GAAGA 2 cut(s) 130, 296
MfeI CAATTG 1 cut(s) 324
MflI RGATCY 1 cut(s) 32
MhlI GDGCHC 4 cut(s) 55, 228, 287, 580
MluCI AATT 2 cut(s) 324, 435
MlyI GAGTC 1 cut(s) 570
MnlI CCTC 7 cut(s) 32, 56, 73, 183, 250, 439, 590
MslI CAYNNNNRTG 1 cut(s) 297
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 2 cut(s) 55, 597
MspR9I CCNGG 4 cut(s) 55, 115, 309, 342
MunI CAATTG 1 cut(s) 324
Mva1269I GAATGC 1 cut(s) 404
MvaI CCWGG 3 cut(s) 115, 309, 342
MwoI GCNNNNNNNGC 4 cut(s) 83, 89, 326, 446
NciI CCSGG 1 cut(s) 55
NdeI CATATG 1 cut(s) 294
NdeII GATC 2 cut(s) 32, 195
NlaIII CATG 5 cut(s) 169, 268, 317, 352, 362
NlaIV GGNNCC 2 cut(s) 53, 129
NmeAIII GCCGAG 2 cut(s) 35, 154
NmuCI GTSAC 1 cut(s) 373
NspI RCATGY 1 cut(s) 317
PagI TCATGA 1 cut(s) 165
PciI ACATGT 1 cut(s) 313
PctI GAATGC 1 cut(s) 404
PfeI GAWTC 2 cut(s) 40, 95
PflFI GACNNNGTC 1 cut(s) 464
PfoI TCCNGGA 1 cut(s) 307
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 1 cut(s) 569
PpsI GAGTC 1 cut(s) 569
PscI ACATGT 1 cut(s) 313
Psp6I CCWGG 3 cut(s) 113, 307, 340
PspFI CCCAGC 1 cut(s) 88
PspGI CCWGG 3 cut(s) 113, 307, 340
PspN4I GGNNCC 2 cut(s) 53, 129
PspOMI GGGCCC 1 cut(s) 51
PspPI GGNCC 4 cut(s) 51, 52, 57, 127
PstNI CAGNNNCTG 1 cut(s) 514
PsuI RGATCY 1 cut(s) 32
PsyI GACNNNGTC 1 cut(s) 464
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
RseI CAYNNNNRTG 1 cut(s) 297
SalI GTCGAC 1 cut(s) 526
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 2 cut(s) 32, 195
Sau96I GGNCC 4 cut(s) 51, 52, 57, 127
SchI GAGTC 1 cut(s) 570
ScrFI CCNGG 4 cut(s) 55, 115, 309, 342
SduI GDGCHC 4 cut(s) 55, 228, 287, 580
SetI ASST 8 cut(s) 75, 164, 210, 246, 261, 343, 516, 547
SfaNI GCATC 1 cut(s) 460
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 297
Sse9I AATT 2 cut(s) 324, 435
SsiI CCGC 3 cut(s) 7, 92, 131
SspMI CTAG 1 cut(s) 601
StyD4I CCNGG 4 cut(s) 53, 113, 307, 340
TaaI ACNGT 2 cut(s) 214, 464
TaiI ACGT 1 cut(s) 75
TaqI TCGA 5 cut(s) 98, 194, 468, 527, 564
TasI AATT 2 cut(s) 324, 435
TfiI GAWTC 2 cut(s) 40, 95
TscAI CASTG 4 cut(s) 112, 217, 292, 400
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 3 cut(s) 154, 182, 375
TspRI CASTG 4 cut(s) 112, 217, 292, 400
Tth111I GACNNNGTC 1 cut(s) 464
VpaK11BI GGWCC 1 cut(s) 127
XceI RCATGY 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 527
XspI CTAG 1 cut(s) 601
ZraI GACGTC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.