MD02G1222900.v1.1

ATP synthase subunit delta'

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003919
Physical Location & Seq
Reverse (-)
26006514 .. 26009774
3261 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1222900.v1.1.491

Sequence Viewer

Length: 810 bp
ATGAGTCCAATGGGCCCAAACTACAAATATCAAAGTCCATTGACAAAGCCCACAACAGCCCTCAACAAAATTCGTACCCATTTCTCCTCCATTCCACAAGCCGAAACGGCAGTGCCATTTCCGAAACACAAACCAAAACCCTCCCCCCAACCTCCCATACTCTCTCTCCGACCCTGCGAAACCCGAGCGACCCCTCACTCCGCCACCATGTTCCGCCGAGCCACCACCCTCTTGACCCGATCCACAATTCCCCTCAGGGCCCGGCCCTTCTCCACCGACGTCGCTGAGAGTGTCCCAGCGGACTCGACTTTCACTGACGCCTGGAAGAAAGTGGTCCCGCACATAGACCCCCCCAAGACCCCATTGACCTTCATGAAGCCTCGGCCTGCTACCCCGTCGTCGATCCCCACTAAGCTAACTGTCAACTTTGTGCTCCCTTATGCGTCCGAGCTTTCGGCTAAAGAGGTTGACATGGTAATAATACCAGCGAGCACTGGGCATATGGGTGTTCTTCCTGGACATGTAGCAACAATTGCAGAGTTGAAACCCGGGGTCATGTCGGTACATGAAGGAACTGATGTCACAAAGTATTTTGTCAGCAGTGGTTTTGCATTCATCCACGCCAACTCTATTGCAGACATAATTGCTGTAGAGGCAGTCCCACTTGACCGTGTCGATGCAAGTCTTGTCCAGAAGGGGCTTGCAGAGTTCAACCAGAAGCTGAACTCAGCGTCAACTGACTTGGAGAAAGCCGAAGCCCAGATTGGAGTCGATGTGCATAGTGCCCTCAACTCTGCGCTCACCGGCTAG

Protein Analysis

270

Amino Acids

28.93

Weight (kDa)

9.28

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_DE_N PF02823 139 - 211 1.9e-15 ATP synthase, Delta/Epsilon chain, beta-sandwich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
AciI CCGC 4 cut(s) 201, 214, 299, 338
AclWI GGATC 2 cut(s) 234, 397
AcsI RAATTY 1 cut(s) 69
AcyI GRCGYC 2 cut(s) 279, 318
AfaI GTAC 2 cut(s) 76, 564
AflIII ACRYGT 1 cut(s) 520
AgsI TTSAA 2 cut(s) 544, 712
AjnI CCWGG 2 cut(s) 320, 514
AjuI GAANNNNNNNTTGG 2 cut(s) 747, 779
AluBI AGCT 3 cut(s) 415, 451, 721
AluI AGCT 3 cut(s) 415, 451, 721
Alw21I GWGCWC 2 cut(s) 435, 494
AlwI GGATC 2 cut(s) 234, 397
AlwNI CAGNNNCTG 1 cut(s) 721
Ama87I CYCGRG 2 cut(s) 183, 548
AoxI GGCC 4 cut(s) 13, 258, 263, 383
ApaI GGGCCC 2 cut(s) 17, 262
ApoI RAATTY 1 cut(s) 69
AspLEI GCGC 1 cut(s) 799
AspS9I GGNCC 6 cut(s) 13, 14, 258, 259, 264, 334
AsuC2I CCSGG 3 cut(s) 262, 549, 550
AsuHPI GGTGA 1 cut(s) 793
AvaI CYCGRG 2 cut(s) 183, 548
AvaII GGWCC 1 cut(s) 334
AxyI CCTNAGG 1 cut(s) 254
BaeGI GKGCMC 3 cut(s) 17, 262, 787
BanII GRGCYC 2 cut(s) 17, 262
Bbv12I GWGCWC 2 cut(s) 435, 494
BceAI ACGGC 1 cut(s) 123
BciT130I CCWGG 2 cut(s) 322, 516
BcnI CCSGG 3 cut(s) 262, 549, 550
BfaI CTAG 1 cut(s) 808
BfmI CTRYAG 1 cut(s) 648
BglI GCCNNNNNGGC 1 cut(s) 107
Bme1390I CCNGG 5 cut(s) 262, 322, 516, 549, 550
Bme18I GGWCC 1 cut(s) 334
BmeT110I CYCGRG 2 cut(s) 183, 548
BmgT120I GGNCC 6 cut(s) 13, 14, 258, 259, 264, 334
BmiI GGNNCC 3 cut(s) 15, 260, 336
BmrFI CCNGG 5 cut(s) 262, 322, 516, 549, 550
BmrI ACTGGG 1 cut(s) 504
BmsI GCATC 1 cut(s) 667
BmuI ACTGGG 1 cut(s) 504
BpuMI CCSGG 3 cut(s) 262, 549, 550
BsaHI GRCGYC 2 cut(s) 279, 318
BsaJI CCNNGG 3 cut(s) 380, 548, 549
BsaXI ACNNNNNCTCC 4 cut(s) 150, 180, 759, 789
Bse118I RCCGGY 1 cut(s) 803
Bse1I ACTGG 1 cut(s) 499
Bse21I CCTNAGG 1 cut(s) 254
BseBI CCWGG 2 cut(s) 322, 516
BseDI CCNNGG 3 cut(s) 380, 548, 549
BseGI GGATG 1 cut(s) 615
BseMII CTCAG 3 cut(s) 268, 276, 741
BseNI ACTGG 1 cut(s) 499
BseRI GAGGAG 1 cut(s) 76
BseSI GKGCMC 3 cut(s) 17, 262, 787
BseYI CCCAGC 1 cut(s) 295
BshFI GGCC 4 cut(s) 15, 260, 265, 385
BsiHKAI GWGCWC 2 cut(s) 435, 494
BsiHKCI CYCGRG 2 cut(s) 183, 548
BsiSI CCGG 3 cut(s) 262, 549, 804
BslFI GGGAC 3 cut(s) 278, 320, 644
BsmFI GGGAC 3 cut(s) 278, 320, 644
BsmI GAATGC 1 cut(s) 611
BsnI GGCC 4 cut(s) 15, 260, 265, 385
BsoBI CYCGRG 2 cut(s) 183, 548
Bsp120I GGGCCC 2 cut(s) 13, 258
Bsp1286I GDGCHC 5 cut(s) 17, 262, 435, 494, 787
Bsp143I GATC 2 cut(s) 239, 402
BspACI CCGC 4 cut(s) 201, 214, 299, 338
BspANI GGCC 4 cut(s) 15, 260, 265, 385
BspCNI CTCAG 3 cut(s) 267, 277, 740
BspHI TCATGA 1 cut(s) 372
BspLI GGNNCC 3 cut(s) 15, 260, 336
BspPI GGATC 2 cut(s) 234, 397
BsrFI RCCGGY 1 cut(s) 803
BsrI ACTGG 1 cut(s) 499
BssAI RCCGGY 1 cut(s) 803
BssECI CCNNGG 3 cut(s) 380, 548, 549
BssMI GATC 2 cut(s) 239, 402
BssNI GRCGYC 2 cut(s) 279, 318
Bst2UI CCWGG 2 cut(s) 322, 516
Bst4CI ACNGT 2 cut(s) 421, 671
BstACI GRCGYC 2 cut(s) 279, 318
BstAPI GCANNNNNTGC 1 cut(s) 533
BstC8I GCNNGC 3 cut(s) 387, 490, 702
BstDEI CTNAG 4 cut(s) 254, 285, 411, 727
BstF5I GGATG 1 cut(s) 615
BstHHI GCGC 1 cut(s) 799
BstKTI GATC 2 cut(s) 242, 405
BstMBI GATC 2 cut(s) 239, 402
BstMWI GCNNNNNNNGC 3 cut(s) 107, 533, 653
BstNI CCWGG 2 cut(s) 322, 516
BstNSI RCATGY 1 cut(s) 524
BstSCI CCNGG 5 cut(s) 260, 320, 514, 547, 548
BstSFI CTRYAG 1 cut(s) 648
BstSLI GKGCMC 3 cut(s) 17, 262, 787
Bsu36I CCTNAGG 1 cut(s) 254
BsuRI GGCC 4 cut(s) 15, 260, 265, 385
BtsCI GGATG 1 cut(s) 615
BtsI GCAGTG 2 cut(s) 117, 607
BtsIMutI CAGTG 4 cut(s) 117, 312, 492, 607
Cac8I GCNNGC 3 cut(s) 387, 490, 702
CaiI CAGNNNCTG 1 cut(s) 721
CciI TCATGA 1 cut(s) 372
CfoI GCGC 1 cut(s) 799
Cfr10I RCCGGY 1 cut(s) 803
Cfr13I GGNCC 6 cut(s) 13, 14, 258, 259, 264, 334
Cfr9I CCCGGG 1 cut(s) 548
CseI GACGC 3 cut(s) 326, 432, 720
Csp6I GTAC 2 cut(s) 75, 563
CviAII CATG 6 cut(s) 208, 373, 472, 521, 556, 566
CviQI GTAC 2 cut(s) 75, 563
DdeI CTNAG 4 cut(s) 254, 285, 411, 727
DpnI GATC 2 cut(s) 241, 404
DpnII GATC 2 cut(s) 239, 402
EciI GGCGGA 2 cut(s) 190, 203
Eco24I GRGCYC 2 cut(s) 17, 262
Eco47I GGWCC 1 cut(s) 334
Eco81I CCTNAGG 1 cut(s) 254
Eco88I CYCGRG 2 cut(s) 183, 548
EcoO109I RGGNCCY 1 cut(s) 258
EcoRII CCWGG 2 cut(s) 320, 514
EcoT38I GRGCYC 2 cut(s) 17, 262
FaeI CATG 6 cut(s) 211, 376, 475, 524, 559, 569
FaqI GGGAC 3 cut(s) 278, 320, 644
FatI CATG 6 cut(s) 207, 372, 471, 520, 555, 565
FauI CCCGC 1 cut(s) 345
FauNDI CATATG 1 cut(s) 501
FokI GGATG 1 cut(s) 602
FriOI GRGCYC 2 cut(s) 17, 262
FspBI CTAG 1 cut(s) 808
GlaI GCGC 1 cut(s) 798
GsaI CCCAGC 1 cut(s) 299
HaeIII GGCC 4 cut(s) 15, 260, 265, 385
HapII CCGG 3 cut(s) 262, 549, 804
HgaI GACGC 3 cut(s) 326, 432, 720
HhaI GCGC 1 cut(s) 799
Hin1I GRCGYC 2 cut(s) 279, 318
Hin1II CATG 6 cut(s) 211, 376, 475, 524, 559, 569
Hin6I GCGC 1 cut(s) 797
HinP1I GCGC 1 cut(s) 797
HincII GTYRAC 3 cut(s) 424, 469, 735
HindII GTYRAC 3 cut(s) 424, 469, 735
HinfI GANTC 3 cut(s) 4, 302, 768
HpaII CCGG 3 cut(s) 262, 549, 804
HphI GGTGA 1 cut(s) 793
Hpy166II GTNNAC 3 cut(s) 424, 469, 735
Hpy188I TCNGA 3 cut(s) 123, 170, 448
Hpy188III TCNNGA 3 cut(s) 232, 373, 691
Hpy8I GTNNAC 3 cut(s) 424, 469, 735
Hpy99I CGWCG 4 cut(s) 281, 284, 400, 403
HpyAV CCTTC 4 cut(s) 277, 379, 563, 688
HpyCH4III ACNGT 2 cut(s) 421, 671
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 6 cut(s) 536, 611, 635, 680, 704, 778
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 533, 653
HpyF3I CTNAG 4 cut(s) 254, 285, 411, 727
HpySE526I ACGT 1 cut(s) 279
Hsp92I GRCGYC 2 cut(s) 279, 318
Hsp92II CATG 6 cut(s) 211, 376, 475, 524, 559, 569
HspAI GCGC 1 cut(s) 797
Kzo9I GATC 2 cut(s) 239, 402
LmnI GCTCC 1 cut(s) 438
LweI GCATC 1 cut(s) 667
MaeI CTAG 1 cut(s) 808
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 1 cut(s) 580
MalI GATC 2 cut(s) 241, 404
MboI GATC 2 cut(s) 239, 402
MboII GAAGA 2 cut(s) 337, 503
MfeI CAATTG 1 cut(s) 531
MhlI GDGCHC 5 cut(s) 17, 262, 435, 494, 787
MluCI AATT 4 cut(s) 69, 246, 531, 642
MlyI GAGTC 3 cut(s) 13, 296, 777
MmeI TCCRAC 1 cut(s) 193
MslI CAYNNNNRTG 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 3 cut(s) 262, 549, 804
MspR9I CCNGG 5 cut(s) 262, 322, 516, 549, 550
MunI CAATTG 1 cut(s) 531
Mva1269I GAATGC 1 cut(s) 611
MvaI CCWGG 2 cut(s) 322, 516
MwoI GCNNNNNNNGC 3 cut(s) 107, 533, 653
NciI CCSGG 3 cut(s) 262, 549, 550
NdeI CATATG 1 cut(s) 501
NdeII GATC 2 cut(s) 239, 402
NlaIII CATG 6 cut(s) 211, 376, 475, 524, 559, 569
NlaIV GGNNCC 3 cut(s) 15, 260, 336
NmeAIII GCCGAG 2 cut(s) 242, 361
NmuCI GTSAC 1 cut(s) 580
NspI RCATGY 1 cut(s) 524
PagI TCATGA 1 cut(s) 372
PciI ACATGT 1 cut(s) 520
PctI GAATGC 1 cut(s) 611
PflFI GACNNNGTC 1 cut(s) 671
PfoI TCCNGGA 1 cut(s) 514
PleI GAGTC 3 cut(s) 12, 296, 776
PpsI GAGTC 3 cut(s) 12, 296, 776
PscI ACATGT 1 cut(s) 520
Psp6I CCWGG 2 cut(s) 320, 514
PspFI CCCAGC 1 cut(s) 295
PspGI CCWGG 2 cut(s) 320, 514
PspN4I GGNNCC 3 cut(s) 15, 260, 336
PspOMI GGGCCC 2 cut(s) 13, 258
PspPI GGNCC 6 cut(s) 13, 14, 258, 259, 264, 334
PstNI CAGNNNCTG 1 cut(s) 721
PsyI GACNNNGTC 1 cut(s) 671
RsaI GTAC 2 cut(s) 76, 564
RsaNI GTAC 2 cut(s) 75, 563
RseI CAYNNNNRTG 1 cut(s) 504
Sau3AI GATC 2 cut(s) 239, 402
Sau96I GGNCC 6 cut(s) 13, 14, 258, 259, 264, 334
SchI GAGTC 3 cut(s) 13, 296, 777
ScrFI CCNGG 5 cut(s) 262, 322, 516, 549, 550
SduI GDGCHC 5 cut(s) 17, 262, 435, 494, 787
SetI ASST 7 cut(s) 154, 282, 371, 417, 453, 468, 723
SfaNI GCATC 1 cut(s) 667
SfcI CTRYAG 1 cut(s) 648
SinI GGWCC 1 cut(s) 334
SmaI CCCGGG 1 cut(s) 550
SmiMI CAYNNNNRTG 1 cut(s) 504
Sse9I AATT 4 cut(s) 69, 246, 531, 642
SsiI CCGC 4 cut(s) 201, 214, 299, 338
SspMI CTAG 1 cut(s) 808
StyD4I CCNGG 5 cut(s) 260, 320, 514, 547, 548
TaaI ACNGT 2 cut(s) 421, 671
TaiI ACGT 1 cut(s) 282
TaqI TCGA 4 cut(s) 305, 401, 675, 771
TasI AATT 4 cut(s) 69, 246, 531, 642
TscAI CASTG 4 cut(s) 117, 319, 499, 607
TseFI GTSAC 1 cut(s) 580
Tsp45I GTSAC 1 cut(s) 580
TspDTI ATGAA 4 cut(s) 361, 389, 582, 604
TspMI CCCGGG 1 cut(s) 548
TspRI CASTG 4 cut(s) 117, 319, 499, 607
Tth111I GACNNNGTC 1 cut(s) 671
VpaK11BI GGWCC 1 cut(s) 334
XapI RAATTY 1 cut(s) 69
XceI RCATGY 1 cut(s) 524
XmaI CCCGGG 1 cut(s) 548
XspI CTAG 1 cut(s) 808
ZraI GACGTC 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.