FvH4_7g17640

Vacuolar protein sorting-associated protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006617
Physical Location & Seq
Forward (+)
14917331 .. 14927107
9777 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g17640.t6

Sequence Viewer

Length: 5790 bp
ATGACCAACAAGCTCACACAGCCGGAACCCCAACTCGCCATGGAGCTCGACCTCGACTCCTTCCTCACCTCCCACCTCTCCTCCGATTCCGACTCCGACGCCGACCTCGCCTCCGTCCCTCGCCGCACGATCGACGAAATCCTCAACGACACCGATTCCTCCACGTCACCCTCCCCCTCCTCCTCCCCGCGCAGCAGCTCCGCCTTATCTCAGTTCCTCGCCTCCGATCCCAAACCGCCACAAACCGACGCCGTTTCCGTCGCCTCGGCGCGGTCGAGTCAGTTGGAGGAGAAGTCGGTCCGGCCGGGTTTGAATTTGTATAGTCGAGCTAGGTCCGGTGACTTCCCGGACGATCAGGTCGGGAGAGTTTCGCGGCCGTCGCCGTGGCTGTTGGGAGGTGTGAGGACGAATGCGAAGCCCGGGGCGGCGCTCGCGGCGGCTGCGGCGGCTTCCAGGTCGATGCCGACGCCGCACGCCGCGGCGATCAAGTCGAGGAGGAGCGCCGGGAGCGGGAGCTTCCAGAAGGCTGTGGAGGCTGTCGTGGCTGAGGAATTGGGTGTGAGATCGGAGGATAACAATGCGGTTGGTTCCGAAACGACGGCGATGAGTTCGAATGGAGCAGAGGAGGGTTTTGGTGGTGAATTGGGGAGGAAAGATGAGGTTTTGGAGAGGGAGAGTGTGGTAGATGAAGTTAGTGCTGGCAATGCTGGTGCAGAAGAGGTCAGTAGTGTTAGTTTTGATAAGAATTCGATGAATTTAGATGGAAATGATGGTAAAGATAATGAATTCGATGAGAATGTTGAGGTTGCGGTGGAATCTAATCCGGAATTGGATGAGAACAGTCCTTCTCCGAGACGTAGTGATGTTGAGGATGAGCCTACTGGTGAGGATCAGCAGCATTTTGTTGGTAATGATGATAATGATGAGGTTGGTGATAATGATGATGGTATTAAAGATGGTGATGATCATTTTGATGACGAGGATGGTGCATTGGGAACTTCAATCACTCAGCTTGTGGAGGAGAGAATGGAGCAATTGGAGAGCAGGAGGGTTAGTAAAAAAGCGGAAAAGAAATTGAGAAAGCCGCTTGAAATTGCTGAAGAGCTGGAGAAGAAACAAGCTTCAACTGCTTTGCATTGGGAAGAGGGTGCTGCTGCTCAACCCATGAGGCTTGAGGGTGTTCGGAGAGGGTCCACCACATTGGGATACTTTGATGTTGATGCTAAAAACACCATTACTCGGACCCTTTCTGCTCCGGCTTTGAGGCGTGATCATGGTTCGCCGCAAGTACTGGGAGTTCACAGTAATTACATTGCAATAGGAATGTCGAGAGGGGTTGTCCTTGTTGTCCCCAGTAAATACTCTCCTCATAATGCTGATAACATGGATGCAAAGCTGCTATTTCTTGGATTGCAAGGAGAGCGGTCTTATGCTGCGGTAACTTCTATATCCTTCAATCAGCAAGGGGATCTACTCTTAGCTGGATACGCCGATGGTCACATTACAGTTTGGGATGTTCAGAGGGCATCCGCTGCCAAAGTAATTACAGGAGAACATACAGCTCCAGTTGTACATACTTTTTTCTTGGGGCATGATTCTCAGGTCACACGTAATTTTAAGGCAGTTACTGGTGATAGTAAGGGTCTGGTACTGTTACATTCCTTCTCTGTGGTGCCCCTGCTTAATAGATTCTCGATCAAAACACAGTGCCTTCTAGATGGACAAAAAACAGGCACGACGCTGTCAGCTTCACCACTCATCATTGATGAATCATGCGGAGGCTCTTCACTATCCTCTCAGGGAAATGCGATGGGTTCAGGCAGCAGTATTGGTGGTATGATGGGTGGTGTGGTTGGTGCGGATGCAGGATGGAAACTCTTCAATGAAGGATCCTCTTTGGTTGAAGAAGGTGTGGTCGTATTTGTCACCCATCAAAATGCTCTGGTGGTAAGACTAACTCCCAGTTTGCATGTCTATGCACAACTTTCTAAACCAGAAGGGGTTCGTGAGGGTTCCATGCCCTGTACTGCTTGGAAATGTACAACTCAATTGCTCAATTCACCTACTAATAGTGAGAATGTGCCTGCTGAAGCAGTTGAGAGAGTTTCATTACTTGCAATTGCTTGGGATCGGAAAGTTCAAGTAGCAAAGTTGGTCAAAACAGAGCTGAAAGTATATGGTAAATGGTCTCTTGAAAGTTCAGCCATAGGAGTGGCTTGGTTGGATGATCAGATGCTGGTGGTTCTCACGGTGACTGGACAACTTTGTTTGTTTGCAAAGGATGGAACTGTAATTCACCAAACAAGTTTTTCTCGAGATGGTTTTGGAGGGGATGATCTCATTTCGTATCATACTCACTTCGTCAATGTATTTGGAAACCCTGAAAAAGCTTATAACAATTGCATAGCTGTAAGAGGAGCTTCTGTATATGTACTTGGACCTACTCATCTTATTGTTTCCCGGCTTCTCCCATGGAAGGAACGGATTCAGGTTTTACGAAGAGCTGGTGACTGGATGGGTTCTTTGAACATGGCAATGACAATTTATGATGGCCAAGCTCATGGTGTCGTTGACCTTCCTAGGACTCTGGATGCTGTGCAGGAGGCCATAATGCCTTACCTGGTTGAATTGCTTTTGTCATATGTAGAGGAAGTTTTTTCTTATATATCAGTAGCATTCTGCAACCAAATTGGAAAAATGGATCAGGTTGATGACTTGAACAGACAAAGTAGTTCTGTGCACACCGAAATAAAAGAGCAATACACCCGTGTTGGTGGGGTCGCAGTTGAATTCTGTGTCCATATCAAGAGGACTGACATACTCTTTGATGAAATCTTCTTGAAATTTGTAGCTGTTCAACAGAGAGACACATTTTTGGAATTATTGGAGCCTTATATATTGAAAGACATGCTTGGTTCTCTTCCGCCTGAGATTATGCAAGCTCTGGTGGAACATTACAGCCTCAAAGGGTGGTTACAGCGTGTTGAACAATGTGTCCTTCACATGGATATTTCTTCATTGGATTTCAATCAGGTTGTTAGGTTATGCCGGGAACATGGGTTATATAGTGCCCTGGTGTATCTTTTCAACAAAGGATTGAATGATTTCAGGTCACCATTAGAGGAGCTGTTGGTTGTCTTGCGAAATAGTCAAAAAGAAGGTGCTATGGCCCTTGGATACAGGATGCTAGTTTATTTGAAGTACTGCTTCTCTGGCCTGGCTTTCCCACCAGGACAAGGAAAAATTCCTCCTTTACGCTTGCCATCTCTCAGAACAGAACTTTTGCACTTTTTATTGGAGGGCTCTGATGCCCCTAATTCAAGAGCACTCTCAAGCGAATTCCCTGGAGGAGAACATCTCAACCTTTATTATCTCTTAGAGCTAGATACTGAAGCTACTTTAGATGTTCTGAGATGTGCTTTCTCAAAAAATGAAATCTCCAAACCCGACTTCTCTTCTCAAAATTCAGCTGATGCAGATATAGAGCTTCAGTATGGGAATAACTCAATGGCTCAAAGTCAGGATTCATTGGTCCAGAATACAATTGATACTCTTATTCACATTATTAGTAAGGACGTTCCCCAGAAGGATGGATCTGCTAGCAGTGTTGACCCCGGATCAGTCGTAGCATGGCCTTCAAAGAAAGATATTGATCATCTGTTTGAGTTTATTGCATTTTATGTTGCATGTGGAAAAGCTAATGTCTCGAAGGCTGTACTGTCTCAGATTTTGGAGTATTTGACATCAGAGAATAACTTCCCCTCATCTGTTTCTGGAGATAACATGATTTCAAAAAGAAGAGAGAAGCAGGTTCTTGGCCTTCTTGAGGTAGTGCCAGAGACTGATTGGGATTCATCTTCTGTGTTACAGCTATGTGAGAAAGCACAGTTTTACCAGGTATGTGGCCTAATTCATACCAGCAGACATCAACATCTTGCTGCTCTGGATTGCTATATGAAAGATGCAGCTGAACCGATTCATGCCTTTGCCTTTATCAACAAAATATTGTTGCGGTTGACAGATAAGGAACGTGCTGGTTTTCGATCAGCAGTTATTTCTCGAATTCCAGAGTTATTTGACTTAAACAGAGAGGGTGCATTCTTCTTGGTTATGGATCATTTTACCAGTGAAGAAGGTTCTCATATACTATCTAAACTTCGCTCCCATCCTAAAAGCCTTTTCCTTTACTTAAAAACAGTGATAGAGGTTCATTTATCTGGCACTCTCGACTTTTCAAGTTTAAGAAATAATAATCTCATGGGAGTGAAGGAACAAACAAAAGCAGTTGAGGCTTTCTTGGAAAGGATCTCTAATTTCCCACAGCTTTTGCGTGATAGTCCTATTAATGTGACTGATGATATGATTGAGCTTTATCTGGAGCTGTTATGTCAGTTTGAACGCAAATCAGTTCTCAAATTTCTAGAGACTTTTGATAGCTATCGTGTGGAGCACTGTTTACGCCTCTGCCAGAAATATGCAATAGTAGATGCCTCATCGTTCTTGTTGGAAAGGGTTGGTGATGTTGGGAGTGCACTTTTACTTACACTATCCTCCCTCAATGAAAAATTCATGAAACTTGAAACCGCTGTTGGAAGTCTACCATCAACTGGGGCTTCACGTGGTTCTGCTAGTACAGAGTACTTGAACAAAGCTTTAAAACTACAGGAGGTGAATGACATTGATAGTATATTGCATGCCTGTATTGGATTGTGCCAACGGAACACCCATCGTTTGAATCCTGATGAATCAGAGGCACTTTGGTTTAGATTGCTTGACTCGTTTTGTGAGCCTTTAATGGATTCGTTTAGTGCGGGTACAGTCTCTAAAGGACAAGATCTTAATAGAATGGTAACCAATTCATTGGATTCACAAGAGGATGATTTGAATTTCATAATCAAGTGGAGAATCCCAAAGCCCCATAAAGGTGCTGATGTCTTAAGAAAATTGTTTTCTCGGTTCATCAAGGAGATTGTTGAGGGCATGATGGGATATGTTCGCCTTCCAACTATCATGTCAAAGCTGCTTTCTGACAATGGTAGCCAGGAATTCGGTGATTTTAAATTCACAATATTGGGGATGCTGAGTACGTATGGCTTCGAAAGAAGAATTCTGGATACTGCCAAGTCCCTGATAGAAGATGATACATTCTATACTATGAGCATCCTTAAGAAAGGAGCTTCACATGGGTATGCCCCCCGCAACCAAAAATGTTGCATATGCGATTGCCTTCTTGACAAGAACTCTTCCAGTTACATTCGCATTTTTACTTGTGGTCATGCGACACATCTTAAATGCGAAGTTTCAGAGAATGAAACACCAAGTCGTAGCTCGTCCTCTGGATGCCCTGTTTGTATGCCCAAGACGAAATCTCAGAGGGCTAAAAACAAATCAGCTCTTGCAGAGGAGAGTTTAGTGAACAAATTTTCATCAAGAACCAAAAATACTCATGGAACAACAGTTCACCTACATGAAAGCAATGCATCTGAGAATAGCTATGGGATTCAGCAAATTTCACGATTTGAGATGTTGACCAATTTGCAGAAACATAGTGGATTAGTCGAGATTGAAAATATGCCTCAATTGAGGCTTGCCCCACCAGCCGTGTACCATGAAAAGGTTAAGCACGGACCTGTTCTGTCACCAGGAGAGAGCAGTAGTAATCTTGCGAGAACAGGGAAACAAAGTAAAATCAAGCAACTCAGGGAGGTAAAAGTAAAAGGATCATCTATTCGGTTTCCTTTGAAAACAAATTTATTTGGCAATGGCAAGGACAAGATCAGCAGGCGATGA

Protein Analysis

1930

Amino Acids

212.44

Weight (kDa)

5.56

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_VPS8 PF23410 410 - 758 3.6e-29 Vps8 beta-propeller
VPS8_N PF23306 411 - 575 6.1e-06 VPS8-like, N-terminal beta propeller repeats
Beta-prop_WDR36-Utp21_1st PF25171 437 - 530 6.8e-06 WDR36/Utp21 first beta-propeller
TPR_Vps8 PF12816 958 - 1141 1.4e-48 Vps8 central TPR
TPR_Vps41 PF23556 1452 - 1520 2e-07 Vps41 TPR-like region
TPR_VPS8_2 PF25066 1575 - 1720 5.4e-06 VPS8-like, TPR-like repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2394
AasI GACNNNNNNGTC 1 cut(s) 356
Acc36I ACCTGC 1 cut(s) 3799
AccB1I GGYRCC 1 cut(s) 1672
AccB7I CCANNNNNTGG 1 cut(s) 4607
AccBSI CCGCTC 2 cut(s) 510, 1423
AccI GTMKAC 1 cut(s) 4597
AccII CGCG 5 cut(s) 190, 271, 373, 434, 479
AccIII TCCGGA 1 cut(s) 823
AcoI YGGCCR 3 cut(s) 302, 374, 2551
AcuI CTGAAG 4 cut(s) 1119, 2109, 3411, 3473
AcvI CACGTG 1 cut(s) 4619
AcyI GRCGYC 3 cut(s) 99, 249, 467
AflII CTTAAG 2 cut(s) 4936, 5165
AflIII ACRYGT 1 cut(s) 1607
AjiI CACGTC 1 cut(s) 165
AjnI CCWGG 9 cut(s) 452, 2619, 3072, 3216, 3229, 3343, 3894, 5040, 5641
AjuI GAANNNNNNNTTGG 4 cut(s) 3434, 3466, 4572, 4604
AloI GAACNNNNNNTCC 4 cut(s) 2979, 3011, 5442, 5474
Alw21I GWGCWC 5 cut(s) 48, 2742, 3328, 4452, 4534
Alw26I GTCTC 8 cut(s) 847, 2193, 2859, 3709, 3726, 3833, 4418, 4825
Alw44I GTGCAC 2 cut(s) 2738, 4530
AlwNI CAGNNNCTG 3 cut(s) 1628, 2236, 3842
Ama87I CYCGRG 2 cut(s) 419, 2313
Aor13HI TCCGGA 1 cut(s) 823
AoxI GGCC 9 cut(s) 302, 374, 2551, 2604, 3168, 3214, 3632, 3817, 3904
ApaLI GTGCAC 2 cut(s) 2738, 4530
ArsI GACNNNNNNTTYG 2 cut(s) 2807, 2839
AseI ATTAAT 1 cut(s) 4344
Asp700I GAANNNNTTC 6 cut(s) 1877, 2001, 2484, 3104, 3755, 3975
AspA2I CCTAGG 1 cut(s) 2579
AspLEI GCGC 4 cut(s) 192, 271, 430, 503
AspS9I GGNCC 8 cut(s) 298, 333, 1191, 1242, 2438, 3169, 3532, 5627
AsuC2I CCSGG 8 cut(s) 306, 347, 420, 421, 505, 2461, 3050, 3615
AsuII TTCGAA 2 cut(s) 611, 5097
AsuNHI GCTAGC 1 cut(s) 3599
AvaI CYCGRG 2 cut(s) 419, 2313
AvaII GGWCC 7 cut(s) 298, 333, 1191, 1242, 2438, 3532, 5627
AvrII CCTAGG 1 cut(s) 2579
BaeGI GKGCMC 4 cut(s) 1677, 2742, 3073, 4534
BalI TGGCCA 1 cut(s) 2553
BamHI GGATCC 1 cut(s) 1889
BanI GGYRCC 1 cut(s) 1672
BanII GRGCYC 2 cut(s) 48, 3305
BbrPI CACGTG 1 cut(s) 4619
Bbv12I GWGCWC 5 cut(s) 48, 2742, 3328, 4452, 4534
BbvCI CCTCAGC 1 cut(s) 546
BceAI ACGGC 5 cut(s) 236, 361, 367, 615, 5585
BcgI CGANNNNNNTGC 2 cut(s) 968, 1002
BciT130I CCWGG 9 cut(s) 454, 2621, 3074, 3218, 3231, 3345, 3896, 5042, 5643
BciVI GTATCC 4 cut(s) 1199, 1478, 3170, 5107
BclI TGATCA 4 cut(s) 964, 1270, 2227, 3652
BcnI CCSGG 8 cut(s) 306, 347, 420, 421, 505, 2461, 3050, 3615
BcoDI GTCTC 8 cut(s) 847, 2193, 2859, 3709, 3726, 3833, 4418, 4825
BfaI CTAG 8 cut(s) 330, 1715, 2580, 3188, 3383, 3600, 4421, 4629
BfmI CTRYAG 1 cut(s) 4661
BfoI RGCGCY 2 cut(s) 431, 504
BfrI CTTAAG 2 cut(s) 4936, 5165
BfuAI ACCTGC 1 cut(s) 3799
BfuI GTATCC 4 cut(s) 1199, 1478, 3170, 5107
BglII AGATCT 1 cut(s) 4834
BlnI CCTAGG 1 cut(s) 2579
BmcAI AGTACT 3 cut(s) 1290, 3203, 4640
Bme18I GGWCC 7 cut(s) 298, 333, 1191, 1242, 2438, 3532, 5627
BmeT110I CYCGRG 2 cut(s) 419, 2313
BmgBI CACGTC 1 cut(s) 165
BmgT120I GGNCC 8 cut(s) 298, 333, 1191, 1242, 2438, 3169, 3532, 5627
BmiI GGNNCC 8 cut(s) 27, 589, 1192, 1244, 1674, 1891, 2014, 2889
BmrI ACTGGG 4 cut(s) 1301, 1347, 1956, 4617
BmtI GCTAGC 1 cut(s) 3603
BmuI ACTGGG 4 cut(s) 1301, 1347, 1956, 4617
BplI GAGNNNNNCTC 2 cut(s) 3342, 3374
BpmI CTGGAG 5 cut(s) 1127, 1548, 3366, 3795, 4397
Bpu10I CCTNAGC 1 cut(s) 546
Bpu14I TTCGAA 2 cut(s) 611, 5097
BpuEI CTTGAG 3 cut(s) 1193, 3316, 3845
BpuMI CCSGG 8 cut(s) 306, 347, 420, 421, 505, 2461, 3050, 3615
BsaAI YACGTR 3 cut(s) 1610, 4619, 5088
BsaBI GATNNNNATC 3 cut(s) 3027, 3651, 4437
BsaHI GRCGYC 3 cut(s) 99, 249, 467
BsaI GGTCTC 1 cut(s) 2193
BsaWI WCCGGW 2 cut(s) 335, 823
Bse3DI GCAATG 5 cut(s) 709, 1311, 2541, 5482, 5767
Bse8I GATNNNNATC 3 cut(s) 3027, 3651, 4437
BseAI TCCGGA 1 cut(s) 823
BseBI CCWGG 9 cut(s) 454, 2621, 3074, 3218, 3231, 3345, 3896, 5042, 5643
BseJI GATNNNNATC 3 cut(s) 3027, 3651, 4437
BseMI GCAATG 5 cut(s) 709, 1311, 2541, 5482, 5767
BseSI GKGCMC 4 cut(s) 1677, 2742, 3073, 4534
BseX3I CGGCCG 2 cut(s) 302, 374
BsgI GTGCAG 2 cut(s) 732, 2618
Bsh1236I CGCG 5 cut(s) 190, 271, 373, 434, 479
Bsh1285I CGRYCG 4 cut(s) 132, 275, 305, 377
BshFI GGCC 9 cut(s) 304, 376, 2553, 2606, 3170, 3216, 3634, 3819, 3906
BshNI GGYRCC 1 cut(s) 1672
BsiEI CGRYCG 4 cut(s) 132, 275, 305, 377
BsiHKAI GWGCWC 5 cut(s) 48, 2742, 3328, 4452, 4534
BsiHKCI CYCGRG 2 cut(s) 419, 2313
BslFI GGGAC 3 cut(s) 101, 1334, 5110
BsmAI GTCTC 8 cut(s) 847, 2193, 2859, 3709, 3726, 3833, 4418, 4825
BsmBI CGTCTC 1 cut(s) 847
BsmFI GGGAC 3 cut(s) 101, 1334, 5110
BsmI GAATGC 3 cut(s) 415, 2675, 4097
BsnI GGCC 9 cut(s) 304, 376, 2553, 2606, 3170, 3216, 3634, 3819, 3906
Bso31I GGTCTC 1 cut(s) 2193
BsoBI CYCGRG 2 cut(s) 419, 2313
Bsp119I TTCGAA 2 cut(s) 611, 5097
Bsp1286I GDGCHC 8 cut(s) 48, 1677, 2742, 3073, 3305, 3328, 4452, 4534
Bsp13I TCCGGA 1 cut(s) 823
Bsp1407I TGTACA 2 cut(s) 1570, 2039
Bsp19I CCATGG 2 cut(s) 39, 2471
BspANI GGCC 9 cut(s) 304, 376, 2553, 2606, 3170, 3216, 3634, 3819, 3906
BspEI TCCGGA 1 cut(s) 823
BspFNI CGCG 5 cut(s) 190, 271, 373, 434, 479
BspHI TCATGA 1 cut(s) 4569
BspLI GGNNCC 8 cut(s) 27, 589, 1192, 1244, 1674, 1891, 2014, 2889
BspMI ACCTGC 1 cut(s) 3799
BspOI GCTAGC 1 cut(s) 3603
BspQI GCTCTTC 3 cut(s) 1095, 1789, 2494
BspT104I TTCGAA 2 cut(s) 611, 5097
BspT107I GGYRCC 1 cut(s) 1672
BspTI CTTAAG 2 cut(s) 4936, 5165
BspTNI GGTCTC 1 cut(s) 2193
BsrBI CCGCTC 2 cut(s) 510, 1423
BsrDI GCAATG 5 cut(s) 709, 1311, 2541, 5482, 5767
BsrGI TGTACA 2 cut(s) 1570, 2039
BssNI GRCGYC 3 cut(s) 99, 249, 467
BssT1I CCWWGG 4 cut(s) 39, 2471, 2579, 3172
Bst2UI CCWGG 9 cut(s) 454, 2621, 3074, 3218, 3231, 3345, 3896, 5042, 5643
BstACI GRCGYC 3 cut(s) 99, 249, 467
BstAFI CTTAAG 2 cut(s) 4936, 5165
BstAPI GCANNNNNTGC 1 cut(s) 1532
BstAUI TGTACA 2 cut(s) 1570, 2039
BstBAI YACGTR 3 cut(s) 1610, 4619, 5088
BstBI TTCGAA 2 cut(s) 611, 5097
BstDSI CCRYGG 4 cut(s) 39, 383, 477, 2471
BstEII GGTNACC 2 cut(s) 3111, 4849
BstENI CCTNNNNNAGG 1 cut(s) 3824
BstFNI CGCG 5 cut(s) 190, 271, 373, 434, 479
BstH2I RGCGCY 2 cut(s) 431, 504
BstHHI GCGC 4 cut(s) 192, 271, 430, 503
BstMAI GTCTC 8 cut(s) 847, 2193, 2859, 3709, 3726, 3833, 4418, 4825
BstMCI CGRYCG 4 cut(s) 132, 275, 305, 377
BstNI CCWGG 9 cut(s) 454, 2621, 3074, 3218, 3231, 3345, 3896, 5042, 5643
BstNSI RCATGY 4 cut(s) 1973, 2911, 3690, 4697
BstPI GGTNACC 2 cut(s) 3111, 4849
BstSFI CTRYAG 1 cut(s) 4661
BstSLI GKGCMC 4 cut(s) 1677, 2742, 3073, 4534
BstSNI TACGTA 1 cut(s) 5088
BstUI CGCG 5 cut(s) 190, 271, 373, 434, 479
BstX2I RGATCY 5 cut(s) 1468, 1889, 3593, 4305, 4834
BstXI CCANNNNNNTGG 6 cut(s) 1201, 2212, 2561, 3590, 3902, 4861
BstYI RGATCY 5 cut(s) 1468, 1889, 3593, 4305, 4834
BstZI CGGCCG 2 cut(s) 302, 374
BsuI GTATCC 4 cut(s) 1199, 1478, 3170, 5107
BsuRI GGCC 9 cut(s) 304, 376, 2553, 2606, 3170, 3216, 3634, 3819, 3906
BtgI CCRYGG 4 cut(s) 39, 383, 477, 2471
BtgZI GCGATG 2 cut(s) 617, 1823
BtrI CACGTC 1 cut(s) 165
BtsI GCAGTG 1 cut(s) 3610
BtsIMutI CAGTG 5 cut(s) 1712, 3610, 4132, 4203, 4450
BveI ACCTGC 1 cut(s) 3799
CaiI CAGNNNCTG 3 cut(s) 1628, 2236, 3842
CciI TCATGA 1 cut(s) 4569
CfoI GCGC 4 cut(s) 192, 271, 430, 503
Cfr13I GGNCC 8 cut(s) 298, 333, 1191, 1242, 2438, 3169, 3532, 5627
Cfr42I CCGCGG 1 cut(s) 480
Cfr9I CCCGGG 1 cut(s) 419
CpoI CGGWCCG 1 cut(s) 298
CseI GACGC 4 cut(s) 107, 257, 475, 1747
CsiI ACCWGGT 2 cut(s) 2619, 3894
CspCI CAANNNNNGTGG 2 cut(s) 4308, 4343
CspI CGGWCCG 1 cut(s) 298
DraI TTTAAA 2 cut(s) 4656, 5059
DrdI GACNNNNNNGTC 1 cut(s) 356
DseDI GACNNNNNNGTC 1 cut(s) 356
EaeI YGGCCR 3 cut(s) 302, 374, 2551
EagI CGGCCG 2 cut(s) 302, 374
EciI GGCGGA 2 cut(s) 190, 2913
Ecl136II GAGCTC 1 cut(s) 46
EclXI CGGCCG 2 cut(s) 302, 374
Eco105I TACGTA 1 cut(s) 5088
Eco130I CCWWGG 4 cut(s) 39, 2471, 2579, 3172
Eco24I GRGCYC 2 cut(s) 48, 3305
Eco31I GGTCTC 1 cut(s) 2193
Eco47I GGWCC 7 cut(s) 298, 333, 1191, 1242, 2438, 3532, 5627
Eco52I CGGCCG 2 cut(s) 302, 374
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 4 cut(s) 1119, 2109, 3411, 3473
Eco72I CACGTG 1 cut(s) 4619
Eco88I CYCGRG 2 cut(s) 419, 2313
Eco91I GGTNACC 2 cut(s) 3111, 4849
EcoICRI GAGCTC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 3824
EcoO65I GGTNACC 2 cut(s) 3111, 4849
EcoRI GAATTC 7 cut(s) 745, 785, 2789, 3338, 4062, 5045, 5106
EcoRII CCWGG 9 cut(s) 452, 2619, 3072, 3216, 3229, 3343, 3894, 5040, 5641
EcoT14I CCWWGG 4 cut(s) 39, 2471, 2579, 3172
EcoT22I ATGCAT 1 cut(s) 5482
EcoT38I GRGCYC 2 cut(s) 48, 3305
ErhI CCWWGG 4 cut(s) 39, 2471, 2579, 3172
Esp3I CGTCTC 1 cut(s) 847
FalI AAGNNNNNCTT 6 cut(s) 2404, 2436, 2895, 2927, 3191, 3223
FaqI GGGAC 3 cut(s) 101, 1334, 5110
FauI CCCGC 4 cut(s) 195, 503, 4804, 5204
FauNDI CATATG 2 cut(s) 2641, 5216
FbaI TGATCA 4 cut(s) 964, 1270, 2227, 3652
FblI GTMKAC 1 cut(s) 4597
FriOI GRGCYC 2 cut(s) 48, 3305
FspBI CTAG 8 cut(s) 330, 1715, 2580, 3188, 3383, 3600, 4421, 4629
GlaI GCGC 4 cut(s) 191, 270, 429, 502
GsuI CTGGAG 5 cut(s) 1127, 1548, 3366, 3795, 4397
HaeII RGCGCY 2 cut(s) 431, 504
HaeIII GGCC 9 cut(s) 304, 376, 2553, 2606, 3170, 3216, 3634, 3819, 3906
HgaI GACGC 4 cut(s) 107, 257, 475, 1747
HhaI GCGC 4 cut(s) 192, 271, 430, 503
Hin1I GRCGYC 3 cut(s) 99, 249, 467
Hin6I GCGC 4 cut(s) 190, 269, 428, 501
HinP1I GCGC 4 cut(s) 190, 269, 428, 501
HincII GTYRAC 4 cut(s) 2572, 3610, 4017, 5529
HindII GTYRAC 4 cut(s) 2572, 3610, 4017, 5529
HindIII AAGCTT 3 cut(s) 1119, 2388, 4650
Hpy99I CGWCG 8 cut(s) 101, 137, 251, 263, 382, 469, 601, 1741
HpyCH4IV ACGT 7 cut(s) 164, 856, 1609, 3576, 4030, 4618, 5087
HpySE526I ACGT 7 cut(s) 164, 856, 1609, 3576, 4030, 4618, 5087
Hsp92I GRCGYC 3 cut(s) 99, 249, 467
HspAI GCGC 4 cut(s) 190, 269, 428, 501
Kpn2I TCCGGA 1 cut(s) 823
Ksp22I TGATCA 4 cut(s) 964, 1270, 2227, 3652
KspI CCGCGG 1 cut(s) 480
LguI GCTCTTC 3 cut(s) 1095, 1789, 2494
MabI ACCWGGT 2 cut(s) 2619, 3894
MaeI CTAG 8 cut(s) 330, 1715, 2580, 3188, 3383, 3600, 4421, 4629
MaeII ACGT 7 cut(s) 164, 856, 1609, 3576, 4030, 4618, 5087
MbiI CCGCTC 2 cut(s) 510, 1423
MfeI CAATTG 6 cut(s) 1034, 2048, 2118, 2398, 3543, 5579
MflI RGATCY 5 cut(s) 1468, 1889, 3593, 4305, 4834
MhlI GDGCHC 8 cut(s) 48, 1677, 2742, 3073, 3305, 3328, 4452, 4534
MlsI TGGCCA 1 cut(s) 2553
MluNI TGGCCA 1 cut(s) 2553
MlyI GAGTC 5 cut(s) 50, 86, 286, 2578, 4769
MmeI TCCRAC 7 cut(s) 114, 120, 264, 2202, 4485, 4570, 5027
Mox20I TGGCCA 1 cut(s) 2553
Mph1103I ATGCAT 1 cut(s) 5482
MroI TCCGGA 1 cut(s) 823
MroXI GAANNNNTTC 6 cut(s) 1877, 2001, 2484, 3104, 3755, 3975
MscI TGGCCA 1 cut(s) 2553
MslI CAYNNNNRTG 9 cut(s) 972, 1935, 1974, 2210, 2534, 4262, 4923, 5187, 5466
Msp20I TGGCCA 1 cut(s) 2553
MspA1I CMGCKG 5 cut(s) 479, 1532, 3470, 3968, 4586
MspCI CTTAAG 2 cut(s) 4936, 5165
MunI CAATTG 6 cut(s) 1034, 2048, 2118, 2398, 3543, 5579
Mva1269I GAATGC 3 cut(s) 415, 2675, 4097
MvaI CCWGG 9 cut(s) 454, 2621, 3074, 3218, 3231, 3345, 3896, 5042, 5643
MvnI CGCG 5 cut(s) 190, 271, 373, 434, 479
NciI CCSGG 8 cut(s) 306, 347, 420, 421, 505, 2461, 3050, 3615
NcoI CCATGG 2 cut(s) 39, 2471
NdeI CATATG 2 cut(s) 2641, 5216
NheI GCTAGC 1 cut(s) 3599
NlaIV GGNNCC 8 cut(s) 27, 589, 1192, 1244, 1674, 1891, 2014, 2889
NmeAIII GCCGAG 1 cut(s) 245
NsiI ATGCAT 1 cut(s) 5482
NspI RCATGY 4 cut(s) 1973, 2911, 3690, 4697
NspV TTCGAA 2 cut(s) 611, 5097
PaeI GCATGC 1 cut(s) 4697
PaeR7I CTCGAG 1 cut(s) 2313
PagI TCATGA 1 cut(s) 4569
PciSI GCTCTTC 3 cut(s) 1095, 1789, 2494
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 272
PctI GAATGC 3 cut(s) 415, 2675, 4097
PdmI GAANNNNTTC 6 cut(s) 1877, 2001, 2484, 3104, 3755, 3975
PflFI GACNNNGTC 1 cut(s) 1741
PflMI CCANNNNNTGG 1 cut(s) 4607
PfoI TCCNGGA 1 cut(s) 345
Ple19I CGATCG 1 cut(s) 132
PleI GAGTC 5 cut(s) 50, 86, 285, 2578, 4769
PmaCI CACGTG 1 cut(s) 4619
PmlI CACGTG 1 cut(s) 4619
PpsI GAGTC 5 cut(s) 50, 86, 285, 2578, 4769
Ppu21I YACGTR 3 cut(s) 1610, 4619, 5088
PshBI ATTAAT 1 cut(s) 4344
PsiI TTATAA 1 cut(s) 2394
Psp124BI GAGCTC 1 cut(s) 48
Psp6I CCWGG 9 cut(s) 452, 2619, 3072, 3216, 3229, 3343, 3894, 5040, 5641
PspCI CACGTG 1 cut(s) 4619
PspEI GGTNACC 2 cut(s) 3111, 4849
PspGI CCWGG 9 cut(s) 452, 2619, 3072, 3216, 3229, 3343, 3894, 5040, 5641
PspN4I GGNNCC 8 cut(s) 27, 589, 1192, 1244, 1674, 1891, 2014, 2889
PspPI GGNCC 8 cut(s) 298, 333, 1191, 1242, 2438, 3169, 3532, 5627
PsrI GAACNNNNNNTAC 4 cut(s) 1281, 1313, 5425, 5457
PstNI CAGNNNCTG 3 cut(s) 1628, 2236, 3842
PsuI RGATCY 5 cut(s) 1468, 1889, 3593, 4305, 4834
PsyI GACNNNGTC 1 cut(s) 1741
PvuI CGATCG 1 cut(s) 132
PvuII CAGCTG 2 cut(s) 3470, 3968
RseI CAYNNNNRTG 9 cut(s) 972, 1935, 1974, 2210, 2534, 4262, 4923, 5187, 5466
Rsr2I CGGWCCG 1 cut(s) 298
RsrII CGGWCCG 1 cut(s) 298
SacI GAGCTC 1 cut(s) 48
SacII CCGCGG 1 cut(s) 480
SapI GCTCTTC 3 cut(s) 1095, 1789, 2494
Sau96I GGNCC 8 cut(s) 298, 333, 1191, 1242, 2438, 3169, 3532, 5627
ScaI AGTACT 3 cut(s) 1290, 3203, 4640
SchI GAGTC 5 cut(s) 50, 86, 286, 2578, 4769
SduI GDGCHC 8 cut(s) 48, 1677, 2742, 3073, 3305, 3328, 4452, 4534
SexAI ACCWGGT 2 cut(s) 2619, 3894
SfcI CTRYAG 1 cut(s) 4661
Sfr274I CTCGAG 1 cut(s) 2313
Sfr303I CCGCGG 1 cut(s) 480
SfuI TTCGAA 2 cut(s) 611, 5097
SgrBI CCGCGG 1 cut(s) 480
SinI GGWCC 7 cut(s) 298, 333, 1191, 1242, 2438, 3532, 5627
SlaI CTCGAG 1 cut(s) 2313
SmaI CCCGGG 1 cut(s) 421
SmiMI CAYNNNNRTG 9 cut(s) 972, 1935, 1974, 2210, 2534, 4262, 4923, 5187, 5466
SmlI CTYRAG 6 cut(s) 1172, 2313, 3331, 3824, 4936, 5165
SmoI CTYRAG 6 cut(s) 1172, 2313, 3331, 3824, 4936, 5165
SnaBI TACGTA 1 cut(s) 5088
SphI GCATGC 1 cut(s) 4697
SspI AATATT 2 cut(s) 4005, 5070
SspMI CTAG 8 cut(s) 330, 1715, 2580, 3188, 3383, 3600, 4421, 4629
SstI GAGCTC 1 cut(s) 48
StyI CCWWGG 4 cut(s) 39, 2471, 2579, 3172
TaiI ACGT 7 cut(s) 167, 859, 1612, 3579, 4033, 4621, 5090
TaqII GACCGA 1 cut(s) 286
TatI WGTACW 9 cut(s) 1288, 1570, 2024, 2039, 2431, 3201, 3715, 4631, 4638
TscAI CASTG 5 cut(s) 1712, 3610, 4132, 4203, 4457
TspGWI ACGGA 5 cut(s) 103, 247, 2497, 4732, 5640
TspMI CCCGGG 1 cut(s) 419
TspRI CASTG 5 cut(s) 1712, 3610, 4132, 4203, 4457
Tth111I GACNNNGTC 1 cut(s) 1741
Van91I CCANNNNNTGG 1 cut(s) 4607
Vha464I CTTAAG 2 cut(s) 4936, 5165
VneI GTGCAC 2 cut(s) 2738, 4530
VpaK11BI GGWCC 7 cut(s) 298, 333, 1191, 1242, 2438, 3532, 5627
VspI ATTAAT 1 cut(s) 4344
XagI CCTNNNNNAGG 1 cut(s) 3824
XbaI TCTAGA 2 cut(s) 1714, 4420
XceI RCATGY 4 cut(s) 1973, 2911, 3690, 4697
XcmI CCANNNNNNNNNTGG 1 cut(s) 3843
XhoI CTCGAG 1 cut(s) 2313
XmaI CCCGGG 1 cut(s) 419
XmaJI CCTAGG 1 cut(s) 2579
XmiI GTMKAC 1 cut(s) 4597
XmnI GAANNNNTTC 6 cut(s) 1877, 2001, 2484, 3104, 3755, 3975
XspI CTAG 8 cut(s) 330, 1715, 2580, 3188, 3383, 3600, 4421, 4629
ZrmI AGTACT 3 cut(s) 1290, 3203, 4640
Zsp2I ATGCAT 1 cut(s) 5482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.