MD07G1159400.v1.1

Vacuolar protein sorting-associated protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006617
Physical Location & Seq
Forward (+)
23325302 .. 23337552
12251 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1159400.v1.1.491

Sequence Viewer

Length: 5862 bp
ATGACCAAGAAGCTCACTCGCTTCGAACCGCAACTCGCCATGGAACTCGACCTTGACTCGTTCCTCAATTCGCACCTCTCCCTCTCCGACGAAGACGACGACGACGACCTTAGCTCCGTCCCCCACCGCACGATTGACGAAATTCTCAACGACACCGATTCGTCCGCCTCATCGTCCCCGCCCTCCTCCACTATCTACCGCCTCAATTCCGATCCCAAACCACCGGAACCTCCCAACGACGCCGTATCGGTCCCCGTCGTTAAGTCCGAGGATGAAAGGGTGGTCCGGCCGAGGTCCAATCTGTACAGTCGGGTCAAGTCCGGGGATTTGTCGGAGGATCCGGCCGGGAGGGTTCCGAAGCCGTCTCCATGGTTGCTCGGAGGCATGAGAACGAATGCGAAGCCCGGGGCGGCCCTGGCTGCGGCTGCGGCGGCCTCAAGGTCGATGCCAACGCCGCACGCCGCGGCAATCAAGTCGAGGAGGAGCGCCGTGAGCGGAGTTATCCAGAAGGTTCTTGAAGGTTCAGAACTGGATGAAAAATCCGAGGTGAGTTCCAATTCTACTGGTGATACGACTGCAATTCGTTCGGCAGTGCTTAGGACAAATTCAAATGAATTACAAGTGGATTTTGGGGGTGAATTGTTGAGAGAAGGCGGGGTTTTGGAGAGCGAAAGCAAGTTAGAGCAGACTTTTCGAGTTGATCGATCTCAAGTTGATGAGGCGAGTGCTGGTAATGCTGTGGAAGAAGAGAAGAATGTAAGTAGTTTTGATGAGAATTTGACAAATTTAGACGCAAATGATGTTAAAGATACGGAATTTAGTAAGAATGTTGAGGTCGTAGAGGAATATAAACAAGATATTCAAGACTTAGATGATAACAGTCCTTGTTCGAAAGAGAGTGATAGCGAGGATAATGATGGTGGCGGTGGTGGCAAGGATGATGGTGATGATGGTGGTGGTAAGGAGTCCGAGGATAACAAAGGCGATATTGGTAACGATAGCGATATTGGTGATGACGATGAATTGGGAAGTTCAATCACTCAACTCGTGGAGGAGAGAATTGGGGAATTAGAGAGCAGGAGGATTAGTAAAAAATCTGAAAAGAAGTTGCGCAAGCCACTTGAAATCGCTGAAGAGCTAGAGAAGAAGCAAGCTTCTAATGCTTTGCATTGGGAAGAGGGTGCCGCTGCTCAACCCATGAGGCTTGAGGGTGTTCGGAGAGGCTCCACCACATTAGGATACTTCAATGTTGATGCTAACAATCCCATTACTCGGACCCTTTCATCTCCGGCGTTAAGGCGTGATCATGGCTCACCGCAAGTCTTGGCGGTTCACAATAATTACATTGCAATAGGAATGGGAAGAGGGTCTATTCTTGTTATTCCTAGCAAGTACTCTGCTCATACTGCTGACAGCATGGATGCAAAGATGCTAATTCTTGGGTTGCAAGGAGAACGGTCTTATGCTGCTGTAACTTCTATGTGCTTCAATCAGCAAGGGGACTTGCTCTTAGCTGGTTATGCTGATGGTCACATTACAGTTTGGGACGTGCAGAGGGCATCGGCTGCCAAAATAATTACCGGAGAACATACAGCTCCAGTTGTACATACGTTGTTTTTGGGGCAGGATTCTCAGGTTACACGTCAATTTAAGGCAGTTACCGGTGATAGTAAGGGTCTGGTATTATTACATTCTTCCTCAGTGGTTCCCCTTCTTAATAGGTTCTCGATTAAAACACAGTGCCTTCTTGATGGACAAAATACAGGCACAGTGCTGTCAGCTTCACCACTCCTGTTTGATGAATTCTGTGGAGGGGCTTCACTGTCTTCTCAGGGAAGTGGGGCGGTTTCAGGCAGCAGTATTGGTGGTATGATGGGGGGTGTGGTTGGTGGAGATGCAGGTTGGAAACTCTTCAATGAGGGATCTTCTTTGGTCGAAGAAGGTGTGGTCGTGTTTGTCACCCATCACACTGTTCTGGTGGTAAGACTAACTCCCACTTTGGAAGTGTATGCACGACTTTCCAAACCAGATGGGGTTCGGGAGGGTTCTATGCCTTGTACTGCTTGGAAATGTACAATACAATCACACAGTTCACCTGCTAGTAGCGAAAATATGCCTGCTGAAGCAGTTGAAAGAGTTTCGTTACTTGCACTTGCTTGGGATCGAAAAGTTCTGGTGGCAAAGTTGGTCAAATCAGAGCTGAAAGTATATGGGAAATGGTCTCTTGAAAGTGCAGCCATAGGTGTGGCTTGGCTAGATGATCAGATGCTGGTGGTTCTCACGGTGACCGGACAACTTTGTTTGTTTGCGAAGGATGGAACTGTGATTCACCAAACAAGTTTTTCTGTTGATGGTTTTGGAGGCGATGATCTCATTGCTTATCATACTCACTTCATTAATATATTTGGAAATCCTGAAAAAGCTTATCACAATTGTGTAGCTGTAAGAGGAGCTTCAGTATATGTACTCGGACCGATGCATCTTATTGTTTCCCGACTTCTCCCATGGAAAGAACGAATTCAGGTTTTACGGGGAGCAGGTGATTGGATGGGTGCCTTGAACATGGCAATGACAATTTATGATGGTCAAGCTCATGGTGTTGTTGACCTTCCTAGAACTCTGGTTGCTGTACAGGAGACCATAATGTCCTACCTGGTTGAGTTGCTATTGTCATATGTAGAGGAAGTATTTTCATATATCTCAGTAGCGTTCTGCAACCAAATTGGAAAAAGGGATCAAGCTGATGACGTAAACAGCAAAAGCAGTTCTATGCACTCTGAAATAAAAGAGCAATACACCCGTGTTGGTGGAGTTGCAGTTGAATTCTGTGTCCATATCAAAAGGACTGACATACTTTTTGATGAAATATTCTCCAAGTTCGTAGCTGTTCAACAGAGAGACACATTTTTGGAGCTGTTGGAGCCTTATATATTGAAGGACATGCTTGGTTCTCTTCCACCTGAGATTATGCAAGCACTGGTAGAGCACTACAGCCGCACAGGGTGGTTACAGCGCGTTGAACAATGTGTCCTTCACATGGATATTTCGTCCTTGGATTTCAATCAGGTTGTTAGGTTATGCAGGGAGCACGGGCTGTATAGTGCTCTGGTGTATCTCTTCAATAAAGGATTGGATGATTTCAGGTCACCCTTAGAGGAGCTGTTGGTGGTCTTGCGAAATAGTCAAAGAGAAGGTGCTACTGCCCTTGGTTACAGGATGCTGGTTTATTTAAAGTACTGCTTTTCAGGCCTTGCTTTTCCACCAGGACAGGGAACAATTCCCCCCTCACGCTTGCCATCTCTGAGAACAGAACTTCTGCAATTTTTGTTGGAGGGTTCTGATGCCCCTAATTCAAGATCAGTCTCAAGTGTAATGCCAGGAGGAGAATATATTAACCTGTATCTTCTGTTAGAGCTAGATACTGAAGCTACTTTGGACGTTCTGAGATGTGCTTTCGTGGAAGATGAAATCTCAAAATCTGACCTTTCTTCTCATGATTCAGATATGCAGGATGGGAACAATTTAATGGCTCAAAATAAAAATTCAATGGTTCAGAACACAGTAGATACTCTTATTCGCATTATTAGTAAGGACAGTTCTCAAACAGATGGATCTCCTAGCAATGATGACACTGGATCAGTAGTAGTGTGGCCTTCAAAGAAAGACATAGATCATCTATTTGAGTTTATTGCATACTATGTTGCATGTGGAAGAGCCACTGTCTCGAAAAGTGTATTGTCTCAGATTTTGGAGTATTTGACCTCAGATAATAACTTTCCACCATGTGTTTCCAGAGATAGCATAACTTCAAAAAGAAGAGAAAAGCAGGTTCTGGGACTTCTTGAGGTAGTGCCTGAGACCGATTGGGATTCATCTTATGTGTTACAGCTATGCGAAGAGGCGCGATTTTACCAGGTTTGTGGCCTAATTCATACTAGCAGACATCAGTATCTTGCTGCTCTGGAGTGCTACATGAAAGGTGTAGAAGAACCCATTCATGCCTTCTCCTTTATCAACAAAACATTATTACAGCTGACTGACAAGGAATGCGCTGCTTTTCGATCAGAAATTATTTCTCGAATTCCAGAGCTTTTTTACTTAAACAGAGAGGGCACATTCTTCTTGGTTATCGATCATTTTACTATTGAAGAAGGTTCTCATATCCTCTCTAAACTTCGTTCCCATCCAAAAAGTCTGTTCCTTTACTTAAAAACAGTGATTGAGGTCCACTTATCCGGCACGCTCGACTTCTCAAGTTTAAGAAAAGATGATCTTGTGAGGGTTAAGGATCAATCGAAAGCAGTTGAGGCGTATCTGGAAAGGATCTCCGATTTCCCAAAGCTTTTGCGTAGTAATCCTGTTAATGTGACTGATGATATGATTGAACTTTATCTGGAGCTGTTATGTCAGTACGAGCGCAATTCAGTTCTCAAATTCTTAGAGACATTCGATAGCTATCGCGTGGAACACTGTTTGCGCCTGTGCCAGAAATATGGGATAACAGATGCTGCTTCTTTCTTGTTGGAAAGGGTTGGTGATGTCGGCAGTGCTCTTTTACTAACACTTTCCACCCTCAGTGACAAATTTATGAAGCTTGATACTGCTGTGGCAAGTCTGGCATCAAGTAATTCTGCTCGTACTGAGCATTTCAGCAATGCTTTAAAATTGGAAGAGGTGAACGACATTAACAGCATTTTGCATGCCTGTATTGGGCTGTGCCAAAGGAACACCCATCGCTTAAATCCTGATGAATCAGAGGCGCTCTGGTTTAGATTACTTGACTCGTTTTGTGAGCCTTTAACAGATTCATTTGATGCTGGAACGGTATCTAAAGGAGAAGATGTTAAAACGACGGTGGCCAAGTCGTTGGACTCAGAAGAGGATGAGATGGCTTTTATAATCAAGTGGAGAATCTCAAAGCTCCATAAAGGCTTTCATATCTTAAGAAAACTGTTCTCTCGGTTCATCAAGGAGATTGTTGAGGGCATGATAGGATATGTTCGCCTTCCGACTATCATGTCCAAACTCCTCTCTGATAATGGCAACCAAGAATTTGGTGATTTTAAATTCACAATTTTGGGGATGCTTAGCACATATGGCTTCGAAAGAAGAATTCTGGATACTGCCAAGTCCCTGATAGAAGATGATACATTCTACACTATGAGCATACTCAAGAAAGGGGCCTCCCATGGGTATGCCCCTCGGAGTCAAATATGTTGTTTATGTGATTGTCTTCTTGACAAGAACTCTTCTAGCTACATTCGCATTTTCAATTGCGGTCATGCAACGCATCTTCAATGTGAAGCTTTAGAGAATGGCGCATCAAGTAGTAGCTCATCCTCTGGATGCCCTGTTTGTATGCCTAAGAAGAAGTCTCAGAGGTCTAGAAGCAAATCGGTCCTTCCAGAGAAGAGTTTGGTGAAGGAGTTTTTGTCAAGAACCCAACAAACACACGGAACAACTTCCCACCCACACGAAAGCAGTGCATCAGAGAATACGTACGGGCTTCAGCAAATATCACGGTTTGATATCTTGACCAATTTGCAAAGAGACCGAGGATTAGTCGAGATTGAAAACATGCCTCAACTAAGGCTCGCACCTCCGGCTGTATATCATGAGAAGGTTCAGAAAGGAACTGTTCTTTCACCGGCGGAAAGCAGCACTGATCTTTCGAGAGTAGGGCAACAAAGTAAAACCAAGCAACTCAGGGACCTAAAAGTAAGGGGGTCGTCTCTCCGGTTACCCTTAAAATCAAACATATTTGGTAATGGTACGCAAGGAGAAGACCAGCAAGCGATGACGTTATTGTCATGTACACAACTAACAACAATACTAGAGTTGGAAATTCTGCAGATGTTGTAA

Protein Analysis

1954

Amino Acids

215.62

Weight (kDa)

5.25

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_VPS8 PF23410 422 - 601 1e-22 Vps8 beta-propeller
VPS8_N PF23306 423 - 598 4e-06 VPS8-like, N-terminal beta propeller repeats
Beta-prop_WDR36-Utp21_1st PF25171 447 - 542 1e-05 WDR36/Utp21 first beta-propeller
TPR_Vps8 PF12816 969 - 1152 3.3e-51 Vps8 central TPR
TPR_Vps41 PF23556 1355 - 1527 2.4e-07 Vps41 TPR-like region
TPR_VPS8_2 PF25066 1574 - 1722 7.2e-06 VPS8-like, TPR-like repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4898
AarI CACCTGC 2 cut(s) 2104, 2528
AasI GACNNNNNNGTC 3 cut(s) 2644, 5017, 5806
Acc16I TGCGCA 1 cut(s) 1112
Acc36I ACCTGC 4 cut(s) 1889, 2104, 2528, 3817
AccB1I GGYRCC 2 cut(s) 1181, 2552
AccBSI CCGCTC 1 cut(s) 495
AccII CGCG 4 cut(s) 464, 3015, 3904, 4461
AcoI YGGCCR 3 cut(s) 287, 342, 4857
AcuI CTGAAG 5 cut(s) 1152, 2142, 2439, 3444, 5492
AcyI GRCGYC 1 cut(s) 240
AdeI CACNNNGTG 2 cut(s) 3003, 3785
AfiI CCNNNNNNNGG 6 cut(s) 421, 1272, 3037, 3269, 4710, 5190
AflII CTTAAG 1 cut(s) 4942
AflIII ACRYGT 1 cut(s) 1640
AgeI ACCGGT 1 cut(s) 1661
AjiI CACGTC 2 cut(s) 1549, 1643
AjnI CCWGG 5 cut(s) 414, 2652, 3262, 3376, 3912
AleI CACNNNNGTG 1 cut(s) 2433
AloI GAACNNNNNNTCC 2 cut(s) 3012, 3044
Alw21I GWGCWC 4 cut(s) 2988, 3090, 3106, 4552
AlwNI CAGNNNCTG 3 cut(s) 2269, 3832, 4508
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 9 cut(s) 287, 342, 411, 432, 3247, 3650, 3922, 4857, 5181
ArsI GACNNNNNNTTYG 2 cut(s) 2840, 2872
AseI ATTAAT 1 cut(s) 2397
AsiGI ACCGGT 1 cut(s) 1661
Asp700I GAANNNNTTC 4 cut(s) 1910, 2517, 3993, 5461
AspLEI GCGC 9 cut(s) 488, 1113, 3015, 3904, 4052, 4419, 4479, 4762, 5321
AsuC2I CCSGG 4 cut(s) 322, 346, 405, 406
AsuII TTCGAA 3 cut(s) 24, 890, 5103
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 8 cut(s) 250, 283, 294, 1275, 2471, 4225, 5398, 5710
BaeGI GKGCMC 1 cut(s) 4115
BaeI ACNNNNGTAYC 4 cut(s) 3932, 3965, 4590, 4623
BalI TGGCCA 1 cut(s) 4859
BamHI GGATCC 1 cut(s) 337
BanI GGYRCC 2 cut(s) 1181, 2552
BauI CACGAG 1 cut(s) 1046
BbsI GAAGAC 4 cut(s) 99, 1818, 5225, 5790
Bbv12I GWGCWC 4 cut(s) 2988, 3090, 3106, 4552
BceAI ACGGC 3 cut(s) 227, 346, 473
BcgI CGANNNNNNTGC 8 cut(s) 456, 490, 567, 601, 674, 708, 5465, 5499
BciT130I CCWGG 5 cut(s) 416, 2654, 3264, 3378, 3914
BciVI GTATCC 2 cut(s) 1232, 5113
BclI TGATCA 2 cut(s) 1303, 2260
BcnI CCSGG 4 cut(s) 322, 346, 405, 406
BfmI CTRYAG 2 cut(s) 2989, 5849
BfoI RGCGCY 2 cut(s) 489, 4763
BfrI CTTAAG 1 cut(s) 4942
BfuAI ACCTGC 4 cut(s) 1889, 2104, 2528, 3817
BfuI GTATCC 2 cut(s) 1232, 5113
BlpI GCTNAGC 1 cut(s) 5087
BmcAI AGTACT 2 cut(s) 1394, 3236
Bme1390I CCNGG 9 cut(s) 322, 346, 405, 406, 416, 2654, 3264, 3378, 3914
Bme18I GGWCC 8 cut(s) 250, 283, 294, 1275, 2471, 4225, 5398, 5710
BmeT110I CYCGRG 1 cut(s) 404
BmgBI CACGTC 2 cut(s) 1549, 1643
BmrFI CCNGG 9 cut(s) 322, 346, 405, 406, 416, 2654, 3264, 3378, 3914
BpiI GAAGAC 4 cut(s) 99, 1818, 5225, 5790
BpmI CTGGAG 3 cut(s) 1581, 3983, 4415
Bpu10I CCTNAGC 2 cut(s) 110, 596
Bpu1102I GCTNAGC 1 cut(s) 5087
Bpu14I TTCGAA 3 cut(s) 24, 890, 5103
BpuEI CTTGAG 7 cut(s) 421, 693, 1226, 3349, 3863, 4237, 5156
BpuMI CCSGG 4 cut(s) 322, 346, 405, 406
Bsa29I ATCGAT 2 cut(s) 703, 4131
BsaAI YACGTR 1 cut(s) 5499
BsaBI GATNNNNATC 2 cut(s) 3060, 4455
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 4 cut(s) 2226, 2630, 3851, 5544
BsaWI WCCGGW 5 cut(s) 223, 1580, 1661, 2288, 5736
Bsc4I CCNNNNNNNGG 6 cut(s) 421, 1272, 3037, 3269, 4710, 5190
Bse118I RCCGGY 2 cut(s) 1661, 5647
Bse1I ACTGG 5 cut(s) 534, 568, 1598, 2982, 3637
Bse3DI GCAATG 5 cut(s) 1344, 2373, 2574, 3628, 4660
Bse8I GATNNNNATC 2 cut(s) 3060, 4455
BseBI CCWGG 5 cut(s) 416, 2654, 3264, 3378, 3914
BseCI ATCGAT 2 cut(s) 703, 4131
BseJI GATNNNNATC 2 cut(s) 3060, 4455
BseLI CCNNNNNNNGG 6 cut(s) 421, 1272, 3037, 3269, 4710, 5190
BseMI GCAATG 5 cut(s) 1344, 2373, 2574, 3628, 4660
BseNI ACTGG 5 cut(s) 534, 568, 1598, 2982, 3637
BseRI GAGGAG 8 cut(s) 175, 493, 496, 1067, 2463, 3170, 3396, 5018
BseSI GKGCMC 1 cut(s) 4115
BseX3I CGGCCG 2 cut(s) 287, 342
BsgI GTGCAG 2 cut(s) 1571, 2253
Bsh1236I CGCG 4 cut(s) 464, 3015, 3904, 4461
Bsh1285I CGRYCG 2 cut(s) 290, 345
BshFI GGCC 9 cut(s) 289, 344, 413, 434, 3249, 3652, 3924, 4859, 5183
BshNI GGYRCC 2 cut(s) 1181, 2552
BshTI ACCGGT 1 cut(s) 1661
BshVI ATCGAT 2 cut(s) 703, 4131
BsiEI CGRYCG 2 cut(s) 290, 345
BsiHKAI GWGCWC 4 cut(s) 2988, 3090, 3106, 4552
BsiHKCI CYCGRG 1 cut(s) 404
BsiWI CGTACG 1 cut(s) 5499
BslFI GGGAC 8 cut(s) 104, 160, 236, 1514, 1559, 3849, 5116, 5723
BslI CCNNNNNNNGG 6 cut(s) 421, 1272, 3037, 3269, 4710, 5190
BsmBI CGTCTC 2 cut(s) 369, 5736
BsmFI GGGAC 8 cut(s) 104, 160, 236, 1514, 1559, 3849, 5116, 5723
BsmI GAATGC 2 cut(s) 400, 4052
BsnI GGCC 9 cut(s) 289, 344, 413, 434, 3249, 3652, 3924, 4859, 5183
Bso31I GGTCTC 4 cut(s) 2226, 2630, 3851, 5544
BsoBI CYCGRG 1 cut(s) 404
Bsp119I TTCGAA 3 cut(s) 24, 890, 5103
Bsp1286I GDGCHC 5 cut(s) 2988, 3090, 3106, 4115, 4552
Bsp1407I TGTACA 5 cut(s) 303, 1603, 2072, 2629, 5813
Bsp1720I GCTNAGC 1 cut(s) 5087
Bsp19I CCATGG 4 cut(s) 39, 368, 2504, 5188
BspANI GGCC 9 cut(s) 289, 344, 413, 434, 3249, 3652, 3924, 4859, 5183
BspDI ATCGAT 2 cut(s) 703, 4131
BspFNI CGCG 4 cut(s) 464, 3015, 3904, 4461
BspHI TCATGA 2 cut(s) 3493, 5614
BspMAI CTGCAG 1 cut(s) 5853
BspMI ACCTGC 4 cut(s) 1889, 2104, 2528, 3817
BspQI GCTCTTC 2 cut(s) 1128, 3706
BspT104I TTCGAA 3 cut(s) 24, 890, 5103
BspT107I GGYRCC 2 cut(s) 1181, 2552
BspTI CTTAAG 1 cut(s) 4942
BspTNI GGTCTC 4 cut(s) 2226, 2630, 3851, 5544
BsrBI CCGCTC 1 cut(s) 495
BsrDI GCAATG 5 cut(s) 1344, 2373, 2574, 3628, 4660
BsrFI RCCGGY 2 cut(s) 1661, 5647
BsrGI TGTACA 5 cut(s) 303, 1603, 2072, 2629, 5813
BsrI ACTGG 5 cut(s) 534, 568, 1598, 2982, 3637
BssAI RCCGGY 2 cut(s) 1661, 5647
BssNI GRCGYC 1 cut(s) 240
BssSI CACGAG 1 cut(s) 1046
BssT1I CCWWGG 6 cut(s) 39, 368, 2504, 3051, 3205, 5188
Bst2BI CACGAG 1 cut(s) 1046
Bst2UI CCWGG 5 cut(s) 416, 2654, 3264, 3378, 3914
BstACI GRCGYC 1 cut(s) 240
BstAFI CTTAAG 1 cut(s) 4942
BstAPI GCANNNNNTGC 1 cut(s) 1565
BstAUI TGTACA 5 cut(s) 303, 1603, 2072, 2629, 5813
BstBAI YACGTR 1 cut(s) 5499
BstBI TTCGAA 3 cut(s) 24, 890, 5103
BstDSI CCRYGG 5 cut(s) 39, 368, 462, 2504, 5188
BstEII GGTNACC 3 cut(s) 2284, 3144, 5739
BstFNI CGCG 4 cut(s) 464, 3015, 3904, 4461
BstH2I RGCGCY 2 cut(s) 489, 4763
BstHHI GCGC 9 cut(s) 488, 1113, 3015, 3904, 4052, 4419, 4479, 4762, 5321
BstMCI CGRYCG 2 cut(s) 290, 345
BstNI CCWGG 5 cut(s) 416, 2654, 3264, 3378, 3914
BstNSI RCATGY 4 cut(s) 2944, 3708, 4703, 5581
BstPI GGTNACC 3 cut(s) 2284, 3144, 5739
BstSCI CCNGG 9 cut(s) 320, 344, 403, 404, 414, 2652, 3262, 3376, 3912
BstSFI CTRYAG 2 cut(s) 2989, 5849
BstSLI GKGCMC 1 cut(s) 4115
BstSNI TACGTA 1 cut(s) 5499
BstUI CGCG 4 cut(s) 464, 3015, 3904, 4461
BstV2I GAAGAC 4 cut(s) 99, 1818, 5225, 5790
BstX2I RGATCY 4 cut(s) 337, 1922, 3611, 4323
BstXI CCANNNNNNTGG 5 cut(s) 2245, 3920, 4493, 4867, 5054
BstYI RGATCY 4 cut(s) 337, 1922, 3611, 4323
BstZI CGGCCG 2 cut(s) 287, 342
Bsu15I ATCGAT 2 cut(s) 703, 4131
BsuI GTATCC 2 cut(s) 1232, 5113
BsuRI GGCC 9 cut(s) 289, 344, 413, 434, 3249, 3652, 3924, 4859, 5183
BsuTUI ATCGAT 2 cut(s) 703, 4131
BtgI CCRYGG 5 cut(s) 39, 368, 462, 2504, 5188
BtgZI GCGATG 3 cut(s) 2379, 4718, 5810
BtrI CACGTC 2 cut(s) 1549, 1643
BtsI GCAGTG 3 cut(s) 597, 4552, 5488
BveI ACCTGC 4 cut(s) 1889, 2104, 2528, 3817
CaiI CAGNNNCTG 3 cut(s) 2269, 3832, 4508
CciI TCATGA 2 cut(s) 3493, 5614
CfoI GCGC 9 cut(s) 488, 1113, 3015, 3904, 4052, 4419, 4479, 4762, 5321
Cfr10I RCCGGY 2 cut(s) 1661, 5647
Cfr42I CCGCGG 1 cut(s) 465
Cfr9I CCCGGG 1 cut(s) 404
ClaI ATCGAT 2 cut(s) 703, 4131
CpoI CGGWCCG 1 cut(s) 2471
CseI GACGC 2 cut(s) 248, 800
CsiI ACCWGGT 2 cut(s) 2652, 3912
CspAI ACCGGT 1 cut(s) 1661
CspI CGGWCCG 1 cut(s) 2471
DraI TTTAAA 3 cut(s) 3231, 4662, 5065
DraIII CACNNNGTG 2 cut(s) 3003, 3785
DrdI GACNNNNNNGTC 3 cut(s) 2644, 5017, 5806
DseDI GACNNNNNNGTC 3 cut(s) 2644, 5017, 5806
EaeI YGGCCR 3 cut(s) 287, 342, 4857
EagI CGGCCG 2 cut(s) 287, 342
EciI GGCGGA 2 cut(s) 154, 5666
EclXI CGGCCG 2 cut(s) 287, 342
Eco105I TACGTA 1 cut(s) 5499
Eco130I CCWWGG 6 cut(s) 39, 368, 2504, 3051, 3205, 5188
Eco147I AGGCCT 1 cut(s) 3249
Eco31I GGTCTC 4 cut(s) 2226, 2630, 3851, 5544
Eco32I GATATC 1 cut(s) 5530
Eco47I GGWCC 8 cut(s) 250, 283, 294, 1275, 2471, 4225, 5398, 5710
Eco52I CGGCCG 2 cut(s) 287, 342
Eco57I CTGAAG 5 cut(s) 1152, 2142, 2439, 3444, 5492
Eco88I CYCGRG 1 cut(s) 404
Eco91I GGTNACC 3 cut(s) 2284, 3144, 5739
EcoO109I RGGNCCY 2 cut(s) 5181, 5710
EcoO65I GGTNACC 3 cut(s) 2284, 3144, 5739
EcoRI GAATTC 5 cut(s) 1802, 2517, 2822, 4080, 5112
EcoRII CCWGG 5 cut(s) 414, 2652, 3262, 3376, 3912
EcoRV GATATC 1 cut(s) 5530
EcoT14I CCWWGG 6 cut(s) 39, 368, 2504, 3051, 3205, 5188
EcoT22I ATGCAT 1 cut(s) 2481
ErhI CCWWGG 6 cut(s) 39, 368, 2504, 3051, 3205, 5188
Esp3I CGTCTC 2 cut(s) 369, 5736
FalI AAGNNNNNCTT 6 cut(s) 2437, 2469, 3224, 3256, 4257, 4289
FaqI GGGAC 8 cut(s) 104, 160, 236, 1514, 1559, 3849, 5116, 5723
FauI CCCGC 2 cut(s) 186, 647
FauNDI CATATG 2 cut(s) 2674, 5095
FbaI TGATCA 2 cut(s) 1303, 2260
FspI TGCGCA 1 cut(s) 1112
GlaI GCGC 9 cut(s) 487, 1112, 3014, 3903, 4051, 4418, 4478, 4761, 5320
GsuI CTGGAG 3 cut(s) 1581, 3983, 4415
HaeII RGCGCY 2 cut(s) 489, 4763
HaeIII GGCC 9 cut(s) 289, 344, 413, 434, 3249, 3652, 3924, 4859, 5183
HgaI GACGC 2 cut(s) 248, 800
HhaI GCGC 9 cut(s) 488, 1113, 3015, 3904, 4052, 4419, 4479, 4762, 5321
Hin1I GRCGYC 1 cut(s) 240
Hin6I GCGC 9 cut(s) 486, 1111, 3013, 3902, 4050, 4417, 4477, 4760, 5319
HinP1I GCGC 9 cut(s) 486, 1111, 3013, 3902, 4050, 4417, 4477, 4760, 5319
HincII GTYRAC 1 cut(s) 2605
HindII GTYRAC 1 cut(s) 2605
HindIII AAGCTT 5 cut(s) 1152, 2421, 4340, 4592, 5304
Hpy166II GTNNAC 7 cut(s) 1333, 2093, 2605, 2752, 4228, 4678, 5816
Hpy8I GTNNAC 7 cut(s) 1333, 2093, 2605, 2752, 4228, 4678, 5816
Hpy99I CGWCG 7 cut(s) 92, 101, 104, 107, 242, 260, 4855
HpyCH4IV ACGT 7 cut(s) 1548, 1610, 1642, 2748, 3438, 5498, 5801
HpySE526I ACGT 7 cut(s) 1548, 1610, 1642, 2748, 3438, 5498, 5801
Hsp92I GRCGYC 1 cut(s) 240
HspAI GCGC 9 cut(s) 486, 1111, 3013, 3902, 4050, 4417, 4477, 4760, 5319
Ksp22I TGATCA 2 cut(s) 1303, 2260
KspI CCGCGG 1 cut(s) 465
LguI GCTCTTC 2 cut(s) 1128, 3706
MabI ACCWGGT 2 cut(s) 2652, 3912
MaeII ACGT 7 cut(s) 1548, 1610, 1642, 2748, 3438, 5498, 5801
MbiI CCGCTC 1 cut(s) 495
MfeI CAATTG 2 cut(s) 2431, 5272
MflI RGATCY 4 cut(s) 337, 1922, 3611, 4323
MhlI GDGCHC 5 cut(s) 2988, 3090, 3106, 4115, 4552
MlsI TGGCCA 1 cut(s) 4859
MluNI TGGCCA 1 cut(s) 4859
MlyI GAGTC 5 cut(s) 50, 974, 4775, 4865, 5215
MmeI TCCRAC 9 cut(s) 111, 312, 1883, 2898, 3309, 4503, 4848, 5033, 5820
Mox20I TGGCCA 1 cut(s) 4859
Mph1103I ATGCAT 1 cut(s) 2481
MroXI GAANNNNTTC 4 cut(s) 1910, 2517, 3993, 5461
MscI TGGCCA 1 cut(s) 4859
MslI CAYNNNNRTG 4 cut(s) 2243, 2433, 2567, 5193
Msp20I TGGCCA 1 cut(s) 4859
MspA1I CMGCKG 3 cut(s) 464, 1187, 4033
MspCI CTTAAG 1 cut(s) 4942
MspR9I CCNGG 9 cut(s) 322, 346, 405, 406, 416, 2654, 3264, 3378, 3914
MunI CAATTG 2 cut(s) 2431, 5272
Mva1269I GAATGC 2 cut(s) 400, 4052
MvaI CCWGG 5 cut(s) 416, 2654, 3264, 3378, 3914
MvnI CGCG 4 cut(s) 464, 3015, 3904, 4461
NciI CCSGG 4 cut(s) 322, 346, 405, 406
NcoI CCATGG 4 cut(s) 39, 368, 2504, 5188
NdeI CATATG 2 cut(s) 2674, 5095
NmeAIII GCCGAG 1 cut(s) 315
NmuCI GTSAC 6 cut(s) 1529, 1957, 2284, 3144, 4366, 4577
NsbI TGCGCA 1 cut(s) 1112
NsiI ATGCAT 1 cut(s) 2481
NspI RCATGY 4 cut(s) 2944, 3708, 4703, 5581
NspV TTCGAA 3 cut(s) 24, 890, 5103
OliI CACNNNNGTG 1 cut(s) 2433
PaeI GCATGC 1 cut(s) 4703
PagI TCATGA 2 cut(s) 3493, 5614
PaqCI CACCTGC 2 cut(s) 2104, 2528
PceI AGGCCT 1 cut(s) 3249
PciSI GCTCTTC 2 cut(s) 1128, 3706
PcsI WCGNNNNNNNCGW 4 cut(s) 96, 102, 264, 700
PctI GAATGC 2 cut(s) 400, 4052
PdmI GAANNNNTTC 4 cut(s) 1910, 2517, 3993, 5461
PfeI GAWTC 8 cut(s) 158, 1628, 2326, 3497, 3869, 4751, 4805, 4911
Pfl23II CGTACG 1 cut(s) 5499
PinAI ACCGGT 1 cut(s) 1661
PleI GAGTC 5 cut(s) 50, 973, 4775, 4865, 5214
PpsI GAGTC 5 cut(s) 50, 973, 4775, 4865, 5214
Ppu21I YACGTR 1 cut(s) 5499
PpuMI RGGWCCY 1 cut(s) 5710
PshBI ATTAAT 1 cut(s) 2397
PsiI TTATAA 1 cut(s) 4898
Psp5II RGGWCCY 1 cut(s) 5710
Psp6I CCWGG 5 cut(s) 414, 2652, 3262, 3376, 3912
PspEI GGTNACC 3 cut(s) 2284, 3144, 5739
PspGI CCWGG 5 cut(s) 414, 2652, 3262, 3376, 3912
PspLI CGTACG 1 cut(s) 5499
PspPPI RGGWCCY 1 cut(s) 5710
PsrI GAACNNNNNNTAC 2 cut(s) 3580, 3612
PstI CTGCAG 1 cut(s) 5853
PstNI CAGNNNCTG 3 cut(s) 2269, 3832, 4508
PsuI RGATCY 4 cut(s) 337, 1922, 3611, 4323
PvuII CAGCTG 1 cut(s) 4033
RseI CAYNNNNRTG 4 cut(s) 2243, 2433, 2567, 5193
Rsr2I CGGWCCG 1 cut(s) 2471
RsrII CGGWCCG 1 cut(s) 2471
SacII CCGCGG 1 cut(s) 465
SapI GCTCTTC 2 cut(s) 1128, 3706
ScaI AGTACT 2 cut(s) 1394, 3236
SchI GAGTC 5 cut(s) 50, 974, 4775, 4865, 5215
ScrFI CCNGG 9 cut(s) 322, 346, 405, 406, 416, 2654, 3264, 3378, 3914
SduI GDGCHC 5 cut(s) 2988, 3090, 3106, 4115, 4552
SexAI ACCWGGT 2 cut(s) 2652, 3912
SfcI CTRYAG 2 cut(s) 2989, 5849
Sfr303I CCGCGG 1 cut(s) 465
SfuI TTCGAA 3 cut(s) 24, 890, 5103
SgrAI CRCCGGYG 1 cut(s) 5647
SgrBI CCGCGG 1 cut(s) 465
SinI GGWCC 8 cut(s) 250, 283, 294, 1275, 2471, 4225, 5398, 5710
SmaI CCCGGG 1 cut(s) 406
SmiMI CAYNNNNRTG 4 cut(s) 2243, 2433, 2567, 5193
SmlI CTYRAG 8 cut(s) 436, 708, 1205, 3364, 3842, 4252, 4942, 5171
SmoI CTYRAG 8 cut(s) 436, 708, 1205, 3364, 3842, 4252, 4942, 5171
SnaBI TACGTA 1 cut(s) 5499
SphI GCATGC 1 cut(s) 4703
SseBI AGGCCT 1 cut(s) 3249
SspI AATATT 1 cut(s) 2868
StuI AGGCCT 1 cut(s) 3249
StyD4I CCNGG 9 cut(s) 320, 344, 403, 404, 414, 2652, 3262, 3376, 3912
StyI CCWWGG 6 cut(s) 39, 368, 2504, 3051, 3205, 5188
TaiI ACGT 7 cut(s) 1551, 1613, 1645, 2751, 3441, 5501, 5804
TaqII GACCGA 5 cut(s) 238, 2488, 3875, 5386, 5568
TatI WGTACW 9 cut(s) 303, 1392, 1603, 2057, 2072, 2464, 2629, 3234, 5813
TauI GCSGC 9 cut(s) 413, 425, 431, 434, 457, 464, 467, 1187, 2997
TfiI GAWTC 8 cut(s) 158, 1628, 2326, 3497, 3869, 4751, 4805, 4911
TseFI GTSAC 6 cut(s) 1529, 1957, 2284, 3144, 4366, 4577
Tsp45I GTSAC 6 cut(s) 1529, 1957, 2284, 3144, 4366, 4577
TspGWI ACGGA 3 cut(s) 106, 827, 5469
TspMI CCCGGG 1 cut(s) 404
Vha464I CTTAAG 1 cut(s) 4942
VpaK11BI GGWCC 8 cut(s) 250, 283, 294, 1275, 2471, 4225, 5398, 5710
VspI ATTAAT 1 cut(s) 2397
XbaI TCTAGA 1 cut(s) 5384
XceI RCATGY 4 cut(s) 2944, 3708, 4703, 5581
XmaI CCCGGG 1 cut(s) 404
XmnI GAANNNNTTC 4 cut(s) 1910, 2517, 3993, 5461
ZrmI AGTACT 2 cut(s) 1394, 3236
Zsp2I ATGCAT 1 cut(s) 2481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.