Rh1DG291000

Vacuolar protein sorting-associated protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0006617
Physical Location & Seq
Forward (+)
51475799 .. 51485467
9669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG291000.1

Sequence Viewer

Length: 5259 bp
ATGACCAAGAAGCTCACACAATCGGAACCACAACTCGCCATGGAGCTCGACCTTGACTCGTTCCTCAACTCACACCTCTCCTCCTCCTCCTCGGACTCCGACCCCGACCTCACCTCCGTCCCTCGCCGTACGATCGACGAAATCCTCAACGACACCGATTCATCCAGGTCAGCATCCCCCTCCTCTTCCCCGCGTAGTTCCACCTTGTCTCAGTTCCTCGCGTCCGATCCCAAACCGCCACAGAGCGACGCCGTTTCGGTCGCCAGCGGAAGAACTGAGGGAGCGGCGCGGTTGAGTCAATTGGAGGAGAAGGCGGTCCGGCCGGGTTTGAATTTGTACAGCCGGGCTAGGTCTGGGGATTTCCCGGACGATTCGGTCGGGAGAGTTTCGAGGCCGTCGCCGTGGCTGTTGGGAGGTGTGAGGACGAATGCGAAGCCCGGGGCCGCGCTCGCGGCGGCTGCGGCGGCTTCGAGGTCGATGCCGACGCCGCACGCCACGGCGATCAAGACGAGGAGGAGCGCCGGGAGCGGGAGTTTCCAGAAGGTTGTGGAGGCTGCGGAATTGGGTGAGAGATCGGAGGGAGGGTCCAATTTTAACGGGGATAGCAATGCGGTTGGTTCCGAAACGACGCCGTCGAATTCGAATGAAGTAGAGGAGGATTTTGGTGGTGAGTTGGGGAGGAAAGATGAGGTTTTGGAGAGGGAGAGGGAGGTAGAGCAGGTGAGTCAGGTTGATGAAGTTAGTGCTGGCAATGCTGGTGAAGAATTGGATGAGAATTCAACAAATTTAGATGCCACTGATGTTAGAGATAATGAATTGGATGAGAATGTTCGGGTTTTGGAGGAAGGTCCACCGGAGGAAGAAGATTTAGATGAAAATAGTCCTTCTTTGAAACATAGAGATGTTGAGGATGAGCCTACTGGTGAGGATGAGCAGCATCTTGGTGGTATTGATCATAATGATGAGGTTGGTGATAATGATAATGATAAAGATGGTGATGATCATTTTGATGATAAGGGTGATGATGCATTGAGAACTTCAATCACTCAACTTGTGGAGGAGAGAATCGAGCAACTGGAGAGCAGGAGGATCAGTAAAAAAGCGGAAAAGAAATCAAGAAAGCCACTTGAAATCGCCGAGGAGCTAGAGAAGAAACAAGCTTCAACTGCTTTGGATTGGGAAGAGGGTGCTGCTGCTCAACCTATGAGACTTGAGGGTGTTCGGAGAGGGTCCACCACATTGGGATACTTCAATGTTGATGCTAAAAATACCATTACGCGGACCCTTTCTGCTCCGGCTTTGAGGCGTGATCATGGTACGCCACAAGTACTGGGAGTTCACAGTAATTACATTGCAATAGGAATGTCGAGAGGGGTTGTCCTTGTTGTCCCCAGTAAATACTCTCCTTATAATGCTGACAACATGGATGCAAAGCTGATATTTCTTGGATTGCAAGGAGAGCGGTCTTATGCTGTGGTAACTTCTATATCCTTCAATCAGCAAGGGGATCTACTCTTAGCTGGATACGCCGATGGTCACATTACAGTTTGGGATGTTCAGAGGGCATCCGCTGCCAAAGTAATTACCGGAGAACATACAGCTCCAGTTGTACATACTTTTTTTTTGGGGCATGATTCTCAGGTCACACGTAATTTTAAGGCAGTTACTGGGGATAGTAAGGGTCTGGTACTGTTACATTCCTTCTCTGTGGTTCCCCTGCTTAATAGATTCTCCATCAAAACACAGTGCCTTCTAGATGGACAAAAAACAGGCACGACGCTGTCAGCTTCACCACTCCTCATTGATGAATTGAGTGGAAGCTCTTCACTTTCCTCTCAGGGAAATGCGATGGGTTCAGGCAGCAGTATTGGTGGCATGATGGGTGGTGTGGTTGGTGGGGATGCAGGTTGGAAACTCTTCAATGAAGGATCCTCTTTGTTTGAAGAAGGTGTGGTCGTATTTGTCACCCATCAAAATGCTTTGGTGGTAAGACTAACTCCCACTTTGCATGTCTATGCACAACTTTCTAAACCAGATGGGGTTCGGGAGGGTTCCATGCCTTGTACTGCTTGGAAATGCACAACTCAATTGCATAATTCACCTACTAATAGTGAGAATACGCCTACTGAAGCAGTTGAAAGAGTTTCAGTACTTGCAATTGCTTGGGATCGGAAAGTTCAGGTAGCAAAGTTGGTCAAATCAGAGCTGAAAGTATATGGTAAATGGTCTCTTGAAAGTGCAGCCATAGGTGTGGCTTGGTTGGATGATCAGATGCTGGTGGTTCTCACGATAACTGGACAACTTTGTTTGTTTGCAAAGGATGGAACTGGAATTCACCAAACAAGTTTTTCTCTAGATGGTTTTAGAGGGGATGATCTCATTTCTTATCATACTCACTTCATCAATGTATTTGGAAACCCTGAAAAAGCTTATAACAATTGCATAGCTGTAAGAGGAGCTTCTGTATATGTACTTGGACCTACTCATCTTATTGTTTCCCGGCTTCTCCCATGGAAGGAACGGATTCAGGTTTTACGGAGAGCTGGTGACTGGATGGGTTCTTTGAACATGGCAATGACAATTTACGATGGCCAAGCTCATGGTGTTGTTGACCTTCCTAGAACTCTGGATGCTGTACAGGAGGTCATAATGCCCTACCTGGTTGAGTTGCTTTTGTCATATGTAGAGGAAGTTTTTTCTTATATTTCAGTAGCATTCTGCAACCAAATTGAAAAAATGGATCAAGTTGATGGCGTAAACAGACAAAGTAGTACTGTGCACACTGAAATTAAAGAGCAATACACCCGTGTTGGTGGGGTTGCAGTTGAATTCTGTGTCCATATCAAGAGGACTGACATACTTTTTGATGAGATCTTCTTGAAGTTTGTAGCTGTTCAACAGAGAGACACATTTTTGGAACTATTGGAGCCTTATATATTGAAGGACATGCTTGGTTCTCTTCCGCCTGAGATTATGCAAGCTCTGGTAGAACATTACAGCCACAAAGGGTGGTTACAGCGTGTTGAGCAATGTGTCCTTCACATGGATATTTCTTCATTGGATTTCAATCAGGTTGTTAGGTTATGCCGCGAGCATGGATTATATAGTGCCCTGGTGTATCTTTTCAACAAAGGATTGAATGATTTCAGGTCACCATTAGAGGAGCTGTTGGTGGTCTTGCGAAATAGTCAAAAAGAAGGTGCTATGGCCCTTGGATACAGGATGCTAGTTTATTTGAAGTACTGCTTTTCAGGCCTTGCTTTTCCACCAGGACAAGGAAAAATTCCTCCTTCACGCTTGCCATCTCTCAGAACAGAACTTTTGCAGTTTTTATTGGAGGGTTCTGATGCCCCTAATTCAAGAGCACTCTCAAGCGAATTCCCAGGAGGAGAATATCTCAACCTTTATTATCTCTTAGAGCTAGATACTGAAGCTACTTTAGATGTTCTGAGATGTGCTTTCACAGAAGTTGAAATCTCGAAATGGGACTTCTCTTCTCAAAATTCAGCTGATGCAGATACAGAGCTGCAGTATGGGAATAATTCAACGGCTCAAAGTCAGGATTCTTTGGTCCAGAATACAATTGATACTCTTATTCACATTATTAGTAAGGACATTCCCCAGAAGGATGGATCTGCTAGCAGTGTTGACTCTGGATCAGTCGTAGAGTGGCCTTCAAAGAAAGATATTGATCATCTATTCGAGTTTATTGCATATTATGTTGCATGTGGAAAAGCTAATGTCTCGAAGACTGTACTTTCTCAGATTTTGGAGTATTTGACATCAGATAATAACTTTCCAACGTCTGTTTCTGGAGATAGCATGATTTCAAAAAGAAGAGAGAAGCAGGTTCTTGGCCTTCTTGAGGTAGTGCCGGAGACTGATTGGAATTCATCTTCTGTGTTACAGCTATGTGAGAAAGCACAATTTTACCAGGTATGTGGCCTAATTCATACCAGCAGACATCAGTATATTGCTGCCCTGGATTGCTATATGAAAGATGCAGCCGAACCCATTCATGCCTTTGCCTTTATCCACAAAACATTGTTACAGTTGACTGACGATGAATGTGCTGCTTTTCGATCAGCAGTTATTTCTAGAATTCCAGAGCTATTTAACTTAAACAGAGAGGGTGCATTCTTCTTGGTCATGGACCATTTTACCAGTGCAGAAGGTTCTCATATCCTATCTGAACTTCGCTCCCATCCACGAAGCCTTTTCCTGTACTTCAAAACAGTGATTGAGGTTCATTTATCTGGGACCCTCGACTTCTCAACTTTAAAAGACGACAATCTTATGGGAGTAAAGGATCAAACAAAAGGAGTTGAGGCTTTCTTGGAAAGGGTCTCTAATTTCCCACAGCTTTTGCGTGATAGTCCTATTAATGTGACTGATGATATGATTGAGCTTTATCTGGAGCTGTTATGCCAGTTTGAATGCAAATCAGTTCTCAAATTCCTAGAGACATATGATAGCTATCGCGTGGAGCACTGTTTACGCCTCTGCCAGAAATATGCAATAATAGATGCTTCATCATTCTTGTTGGAGAGGGTTGGTGATGTTGGAAGTGCACTTTTACTTACACTTTCCACCCTCAATGACAAATTTATCAAGCTTGAAACTGCTATTGGAAGTCTAAATTTAAGTGGGGCTTCACGTGGTTCTGCTAGTACAGAATACTTGAGCAATGCTTTGAAAGTGGAGGAGGTGAATGATATAGACAGTATATTGCATGCATGTATAGGATTGTGCCAACGGAACACCCATCGTCTAAATCCTGATGAATCAGAGGCACTCTGGTTCAAATTGCTTGACTCGTTTTGTGAGCCTTTAATGGATTCGTTTAGTGCTGGTACAGTATCTAAAGGAGAAGATCATAATAGAATGGTAACCAATTCACTGGATTCACAAGAGGATGAAATGAATTTCACAATCAAGTGGAGAATCCCAAAGGCTCATAAAGGTGCTGATATTTTAAGAAAATTGTTTTCTCGGTTCATCAAGGAGATTGTTGAGGGCATGATAGGATATGTTCGCCTTCCAACTATCATGTCAAAGCTCCTTTCTGACAATGGTAGCCAGGAATTTGGTGATTTCAAATTCACAATATTGGGGATGCTTAGCACATATGGCTTTGAAAGAAGAATTCTGGTACATCAGCTTTATTTTAATCTCAGTTATGCTAGTTATGTACATGATGTATTATTGTGTCAAAGTCATTCTTGTGGAACGTACTTACAAGGCAATAAGCATGAATAA

Protein Analysis

1752

Amino Acids

193.7

Weight (kDa)

5.03

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_VPS8 PF23410 423 - 607 7.3e-22 Vps8 beta-propeller
VPS8_N PF23306 424 - 597 4.3e-06 VPS8-like, N-terminal beta propeller repeats
Beta-prop_VPS8 PF23410 647 - 771 4.5e-06 Vps8 beta-propeller
TPR_Vps8 PF12816 971 - 1154 5.1e-51 Vps8 central TPR
TPR_Vps41 PF23556 1466 - 1534 7.9e-07 Vps41 TPR-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1410, 2433
AarI CACCTGC 1 cut(s) 709
AasI GACNNNNNNGTC 1 cut(s) 374
Acc36I ACCTGC 3 cut(s) 709, 1895, 3838
AccBSI CCGCTC 3 cut(s) 284, 528, 1462
AccII CGCG 8 cut(s) 193, 221, 289, 446, 452, 1279, 3090, 4482
AcoI YGGCCR 2 cut(s) 320, 2590
AcuI CTGAAG 2 cut(s) 2148, 3450
AcvI CACGTG 1 cut(s) 4658
AcyI GRCGYC 3 cut(s) 249, 485, 629
AfiI CCNNNNNNNGG 6 cut(s) 528, 1278, 2515, 3043, 3275, 3865
AflIII ACRYGT 1 cut(s) 1646
AjnI CCWGG 8 cut(s) 164, 2658, 3111, 3268, 3382, 3933, 3981, 5079
AjuI GAANNNNNNNTTGG 4 cut(s) 3793, 3825, 4611, 4643
Alw21I GWGCWC 5 cut(s) 48, 2781, 3367, 4491, 4573
Alw26I GTCTC 8 cut(s) 213, 1201, 2232, 2898, 3748, 3872, 4351, 4457
Alw44I GTGCAC 2 cut(s) 2777, 4569
AlwNI CAGNNNCTG 2 cut(s) 1667, 2275
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 9 cut(s) 320, 392, 441, 2590, 3207, 3253, 3671, 3856, 3943
ApaLI GTGCAC 2 cut(s) 2777, 4569
ArsI GACNNNNNNTTYG 2 cut(s) 2846, 2878
AseI ATTAAT 1 cut(s) 4383
Asp700I GAANNNNTTC 5 cut(s) 1822, 1916, 2523, 3143, 4014
AspLEI GCGC 3 cut(s) 289, 448, 521
AsuC2I CCSGG 7 cut(s) 324, 344, 365, 438, 439, 523, 2500
AsuII TTCGAA 1 cut(s) 641
AsuNHI GCTAGC 1 cut(s) 3638
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 9 cut(s) 316, 585, 848, 1230, 1281, 2477, 3571, 4153, 4260
BaeGI GKGCMC 3 cut(s) 2781, 3112, 4573
BalI TGGCCA 1 cut(s) 2592
BamHI GGATCC 1 cut(s) 1928
BanII GRGCYC 1 cut(s) 48
BbrPI CACGTG 1 cut(s) 4658
BbsI GAAGAC 1 cut(s) 3755
Bbv12I GWGCWC 5 cut(s) 48, 2781, 3367, 4491, 4573
BceAI ACGGC 7 cut(s) 111, 236, 379, 385, 513, 616, 3564
BcgI CGANNNNNNTGC 6 cut(s) 460, 494, 3692, 3726, 4052, 4086
BciT130I CCWGG 8 cut(s) 166, 2660, 3113, 3270, 3384, 3935, 3983, 5081
BciVI GTATCC 3 cut(s) 1238, 1517, 3209
BclI TGATCA 5 cut(s) 952, 1000, 1309, 2266, 3691
BcnI CCSGG 7 cut(s) 324, 344, 365, 438, 439, 523, 2500
BcoDI GTCTC 8 cut(s) 213, 1201, 2232, 2898, 3748, 3872, 4351, 4457
BfmI CTRYAG 1 cut(s) 3527
BfoI RGCGCY 1 cut(s) 522
BfuAI ACCTGC 3 cut(s) 709, 1895, 3838
BfuI GTATCC 3 cut(s) 1238, 1517, 3209
BglII AGATCT 1 cut(s) 2871
BlpI GCTNAGC 1 cut(s) 5120
BmcAI AGTACT 4 cut(s) 1329, 2151, 2773, 3242
Bme18I GGWCC 9 cut(s) 316, 585, 848, 1230, 1281, 2477, 3571, 4153, 4260
BmeT110I CYCGRG 1 cut(s) 437
BmrI ACTGGG 3 cut(s) 1340, 1386, 1677
BmtI GCTAGC 1 cut(s) 3642
BmuI ACTGGG 3 cut(s) 1340, 1386, 1677
BpiI GAAGAC 1 cut(s) 3755
BplI GAGNNNNNCTC 2 cut(s) 3381, 3413
BpmI CTGGAG 4 cut(s) 1097, 1587, 3834, 4436
Bpu1102I GCTNAGC 1 cut(s) 5120
Bpu14I TTCGAA 1 cut(s) 641
BpuEI CTTGAG 4 cut(s) 1232, 3355, 3884, 4702
BpuMI CCSGG 7 cut(s) 324, 344, 365, 438, 439, 523, 2500
BsaAI YACGTR 2 cut(s) 1649, 4658
BsaBI GATNNNNATC 3 cut(s) 3066, 3690, 4476
BsaHI GRCGYC 3 cut(s) 249, 485, 629
BsaI GGTCTC 2 cut(s) 2232, 4351
BsaWI WCCGGW 2 cut(s) 853, 1586
Bsc4I CCNNNNNNNGG 6 cut(s) 528, 1278, 2515, 3043, 3275, 3865
Bse3DI GCAATG 6 cut(s) 613, 757, 1350, 2580, 3035, 4693
Bse8I GATNNNNATC 3 cut(s) 3066, 3690, 4476
BseBI CCWGG 8 cut(s) 166, 2660, 3113, 3270, 3384, 3935, 3983, 5081
BseJI GATNNNNATC 3 cut(s) 3066, 3690, 4476
BseLI CCNNNNNNNGG 6 cut(s) 528, 1278, 2515, 3043, 3275, 3865
BseMI GCAATG 6 cut(s) 613, 757, 1350, 2580, 3035, 4693
BseMII CTCAG 9 cut(s) 224, 267, 1652, 1850, 2958, 3322, 3440, 3776, 5188
BseSI GKGCMC 3 cut(s) 2781, 3112, 4573
BseX3I CGGCCG 1 cut(s) 320
BsgI GTGCAG 2 cut(s) 2259, 4188
Bsh1236I CGCG 8 cut(s) 193, 221, 289, 446, 452, 1279, 3090, 4482
Bsh1285I CGRYCG 4 cut(s) 135, 261, 323, 378
BshFI GGCC 9 cut(s) 322, 394, 443, 2592, 3209, 3255, 3673, 3858, 3945
BsiEI CGRYCG 4 cut(s) 135, 261, 323, 378
BsiHKAI GWGCWC 5 cut(s) 48, 2781, 3367, 4491, 4573
BsiHKCI CYCGRG 1 cut(s) 437
BsiWI CGTACG 1 cut(s) 128
BslFI GGGAC 4 cut(s) 104, 1373, 3500, 4273
BslI CCNNNNNNNGG 6 cut(s) 528, 1278, 2515, 3043, 3275, 3865
BsmAI GTCTC 8 cut(s) 213, 1201, 2232, 2898, 3748, 3872, 4351, 4457
BsmFI GGGAC 4 cut(s) 104, 1373, 3500, 4273
BsmI GAATGC 4 cut(s) 433, 2714, 4136, 4442
BsnI GGCC 9 cut(s) 322, 394, 443, 2592, 3209, 3255, 3673, 3858, 3945
Bso31I GGTCTC 2 cut(s) 2232, 4351
BsoBI CYCGRG 1 cut(s) 437
Bsp119I TTCGAA 1 cut(s) 641
Bsp1286I GDGCHC 6 cut(s) 48, 2781, 3112, 3367, 4491, 4573
Bsp1407I TGTACA 4 cut(s) 336, 1609, 2635, 5191
Bsp1720I GCTNAGC 1 cut(s) 5120
Bsp19I CCATGG 2 cut(s) 39, 2510
BspANI GGCC 9 cut(s) 322, 394, 443, 2592, 3209, 3255, 3673, 3858, 3945
BspCNI CTCAG 9 cut(s) 223, 268, 1651, 1849, 2959, 3321, 3441, 3775, 5187
BspFNI CGCG 8 cut(s) 193, 221, 289, 446, 452, 1279, 3090, 4482
BspMAI CTGCAG 1 cut(s) 3531
BspMI ACCTGC 3 cut(s) 709, 1895, 3838
BspOI GCTAGC 1 cut(s) 3642
BspQI GCTCTTC 1 cut(s) 1828
BspT104I TTCGAA 1 cut(s) 641
BspTNI GGTCTC 2 cut(s) 2232, 4351
BsrBI CCGCTC 3 cut(s) 284, 528, 1462
BsrDI GCAATG 6 cut(s) 613, 757, 1350, 2580, 3035, 4693
BsrGI TGTACA 4 cut(s) 336, 1609, 2635, 5191
BssNI GRCGYC 3 cut(s) 249, 485, 629
BssT1I CCWWGG 3 cut(s) 39, 2510, 3211
Bst2UI CCWGG 8 cut(s) 166, 2660, 3113, 3270, 3384, 3935, 3983, 5081
Bst6I CTCTTC 7 cut(s) 190, 1176, 1828, 1922, 2964, 3499, 3832
BstACI GRCGYC 3 cut(s) 249, 485, 629
BstAPI GCANNNNNTGC 1 cut(s) 1571
BstAUI TGTACA 4 cut(s) 336, 1609, 2635, 5191
BstBAI YACGTR 2 cut(s) 1649, 4658
BstBI TTCGAA 1 cut(s) 641
BstC8I GCNNGC 9 cut(s) 265, 450, 492, 748, 2979, 3092, 3299, 3640, 4734
BstDSI CCRYGG 4 cut(s) 39, 401, 495, 2510
BstEII GGTNACC 2 cut(s) 3150, 4888
BstENI CCTNNNNNAGG 1 cut(s) 3863
BstFNI CGCG 8 cut(s) 193, 221, 289, 446, 452, 1279, 3090, 4482
BstH2I RGCGCY 1 cut(s) 522
BstHHI GCGC 3 cut(s) 289, 448, 521
BstMAI GTCTC 8 cut(s) 213, 1201, 2232, 2898, 3748, 3872, 4351, 4457
BstMCI CGRYCG 4 cut(s) 135, 261, 323, 378
BstNI CCWGG 8 cut(s) 166, 2660, 3113, 3270, 3384, 3935, 3983, 5081
BstNSI RCATGY 5 cut(s) 2012, 2950, 3729, 4736, 4740
BstPI GGTNACC 2 cut(s) 3150, 4888
BstSFI CTRYAG 1 cut(s) 3527
BstSLI GKGCMC 3 cut(s) 2781, 3112, 4573
BstUI CGCG 8 cut(s) 193, 221, 289, 446, 452, 1279, 3090, 4482
BstV2I GAAGAC 1 cut(s) 3755
BstX2I RGATCY 4 cut(s) 1507, 1928, 2871, 3632
BstXI CCANNNNNNTGG 7 cut(s) 1240, 2251, 2600, 3629, 3941, 4900, 5087
BstYI RGATCY 4 cut(s) 1507, 1928, 2871, 3632
BstZI CGGCCG 1 cut(s) 320
BsuI GTATCC 3 cut(s) 1238, 1517, 3209
BsuRI GGCC 9 cut(s) 322, 394, 443, 2592, 3209, 3255, 3673, 3858, 3945
BtgI CCRYGG 4 cut(s) 39, 401, 495, 2510
BtgZI GCGATG 1 cut(s) 1862
BtsI GCAGTG 1 cut(s) 3649
BtsIMutI CAGTG 8 cut(s) 795, 1751, 2781, 3649, 4171, 4242, 4489, 4898
BveI ACCTGC 3 cut(s) 709, 1895, 3838
Cac8I GCNNGC 9 cut(s) 265, 450, 492, 748, 2979, 3092, 3299, 3640, 4734
CaiI CAGNNNCTG 2 cut(s) 1667, 2275
CfoI GCGC 3 cut(s) 289, 448, 521
Cfr9I CCCGGG 1 cut(s) 437
CpoI CGGWCCG 1 cut(s) 316
CseI GACGC 5 cut(s) 210, 257, 493, 637, 1786
CsiI ACCWGGT 2 cut(s) 2658, 3933
CspCI CAANNNNNGTGG 2 cut(s) 4347, 4382
CspI CGGWCCG 1 cut(s) 316
DraI TTTAAA 1 cut(s) 4281
DrdI GACNNNNNNGTC 1 cut(s) 374
DseDI GACNNNNNNGTC 1 cut(s) 374
EaeI YGGCCR 2 cut(s) 320, 2590
EagI CGGCCG 1 cut(s) 320
Eam1104I CTCTTC 7 cut(s) 190, 1176, 1828, 1922, 2964, 3499, 3832
EarI CTCTTC 7 cut(s) 190, 1176, 1828, 1922, 2964, 3499, 3832
EciI GGCGGA 1 cut(s) 2952
Ecl136II GAGCTC 1 cut(s) 46
EclXI CGGCCG 1 cut(s) 320
Eco130I CCWWGG 3 cut(s) 39, 2510, 3211
Eco147I AGGCCT 1 cut(s) 3255
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 2 cut(s) 2232, 4351
Eco47I GGWCC 9 cut(s) 316, 585, 848, 1230, 1281, 2477, 3571, 4153, 4260
Eco52I CGGCCG 1 cut(s) 320
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 2148, 3450
Eco72I CACGTG 1 cut(s) 4658
Eco88I CYCGRG 1 cut(s) 437
Eco91I GGTNACC 2 cut(s) 3150, 4888
EcoICRI GAGCTC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 3863
EcoO109I RGGNCCY 1 cut(s) 4260
EcoO65I GGTNACC 2 cut(s) 3150, 4888
EcoRI GAATTC 8 cut(s) 637, 775, 2331, 2828, 3377, 3889, 4101, 5145
EcoRII CCWGG 8 cut(s) 164, 2658, 3111, 3268, 3382, 3933, 3981, 5079
EcoT14I CCWWGG 3 cut(s) 39, 2510, 3211
EcoT22I ATGCAT 2 cut(s) 1030, 4738
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 3 cut(s) 39, 2510, 3211
FalI AAGNNNNNCTT 8 cut(s) 2443, 2475, 3230, 3262, 4636, 4668, 5206, 5238
FaqI GGGAC 4 cut(s) 104, 1373, 3500, 4273
FauI CCCGC 2 cut(s) 198, 521
FauNDI CATATG 3 cut(s) 2680, 4468, 5128
FbaI TGATCA 5 cut(s) 952, 1000, 1309, 2266, 3691
FriOI GRGCYC 1 cut(s) 48
GlaI GCGC 3 cut(s) 288, 447, 520
GsuI CTGGAG 4 cut(s) 1097, 1587, 3834, 4436
HaeII RGCGCY 1 cut(s) 522
HaeIII GGCC 9 cut(s) 322, 394, 443, 2592, 3209, 3255, 3673, 3858, 3945
HgaI GACGC 5 cut(s) 210, 257, 493, 637, 1786
HhaI GCGC 3 cut(s) 289, 448, 521
Hin1I GRCGYC 3 cut(s) 249, 485, 629
Hin6I GCGC 3 cut(s) 287, 446, 519
HinP1I GCGC 3 cut(s) 287, 446, 519
HincII GTYRAC 3 cut(s) 2611, 3649, 4056
HindII GTYRAC 3 cut(s) 2611, 3649, 4056
HindIII AAGCTT 3 cut(s) 1158, 2427, 4613
Hpy99I CGWCG 7 cut(s) 140, 251, 400, 487, 631, 637, 1780
HpyCH4IV ACGT 4 cut(s) 1648, 3803, 4657, 5231
HpySE526I ACGT 4 cut(s) 1648, 3803, 4657, 5231
Hsp92I GRCGYC 3 cut(s) 249, 485, 629
HspAI GCGC 3 cut(s) 287, 446, 519
KflI GGGWCCC 1 cut(s) 4260
Ksp22I TGATCA 5 cut(s) 952, 1000, 1309, 2266, 3691
LguI GCTCTTC 1 cut(s) 1828
MabI ACCWGGT 2 cut(s) 2658, 3933
MaeII ACGT 4 cut(s) 1648, 3803, 4657, 5231
MbiI CCGCTC 3 cut(s) 284, 528, 1462
MfeI CAATTG 5 cut(s) 299, 2087, 2157, 2437, 3582
MflI RGATCY 4 cut(s) 1507, 1928, 2871, 3632
MhlI GDGCHC 6 cut(s) 48, 2781, 3112, 3367, 4491, 4573
MlsI TGGCCA 1 cut(s) 2592
MluNI TGGCCA 1 cut(s) 2592
MlyI GAGTC 6 cut(s) 50, 89, 304, 733, 3644, 4808
MmeI TCCRAC 7 cut(s) 123, 1889, 2241, 3824, 4524, 4543, 5066
Mox20I TGGCCA 1 cut(s) 2592
Mph1103I ATGCAT 2 cut(s) 1030, 4738
MroXI GAANNNNTTC 5 cut(s) 1822, 1916, 2523, 3143, 4014
MscI TGGCCA 1 cut(s) 2592
Msp20I TGGCCA 1 cut(s) 2592
MspA1I CMGCKG 3 cut(s) 267, 1571, 3509
MunI CAATTG 5 cut(s) 299, 2087, 2157, 2437, 3582
Mva1269I GAATGC 4 cut(s) 433, 2714, 4136, 4442
MvaI CCWGG 8 cut(s) 166, 2660, 3113, 3270, 3384, 3935, 3983, 5081
MvnI CGCG 8 cut(s) 193, 221, 289, 446, 452, 1279, 3090, 4482
NciI CCSGG 7 cut(s) 324, 344, 365, 438, 439, 523, 2500
NcoI CCATGG 2 cut(s) 39, 2510
NdeI CATATG 3 cut(s) 2680, 4468, 5128
NheI GCTAGC 1 cut(s) 3638
NmeAIII GCCGAG 1 cut(s) 1162
NmuCI GTSAC 6 cut(s) 1535, 1642, 1963, 2546, 3150, 4387
NsiI ATGCAT 2 cut(s) 1030, 4738
NspI RCATGY 5 cut(s) 2012, 2950, 3729, 4736, 4740
NspV TTCGAA 1 cut(s) 641
PaeI GCATGC 1 cut(s) 4736
PaqCI CACCTGC 1 cut(s) 709
PceI AGGCCT 1 cut(s) 3255
PciSI GCTCTTC 1 cut(s) 1828
PcsI WCGNNNNNNNCGW 1 cut(s) 632
PctI GAATGC 4 cut(s) 433, 2714, 4136, 4442
PdmI GAANNNNTTC 5 cut(s) 1822, 1916, 2523, 3143, 4014
Pfl23II CGTACG 1 cut(s) 128
PflFI GACNNNGTC 2 cut(s) 631, 1780
PfoI TCCNGGA 1 cut(s) 363
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 6 cut(s) 50, 89, 303, 732, 3644, 4808
PmaCI CACGTG 1 cut(s) 4658
PmlI CACGTG 1 cut(s) 4658
PpsI GAGTC 6 cut(s) 50, 89, 303, 732, 3644, 4808
Ppu21I YACGTR 2 cut(s) 1649, 4658
PpuMI RGGWCCY 1 cut(s) 4260
PshBI ATTAAT 1 cut(s) 4383
PsiI TTATAA 2 cut(s) 1410, 2433
Psp124BI GAGCTC 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 4260
Psp6I CCWGG 8 cut(s) 164, 2658, 3111, 3268, 3382, 3933, 3981, 5079
PspCI CACGTG 1 cut(s) 4658
PspEI GGTNACC 2 cut(s) 3150, 4888
PspGI CCWGG 8 cut(s) 164, 2658, 3111, 3268, 3382, 3933, 3981, 5079
PspLI CGTACG 1 cut(s) 128
PspPPI RGGWCCY 1 cut(s) 4260
PsrI GAACNNNNNNTAC 2 cut(s) 1320, 1352
PstI CTGCAG 1 cut(s) 3531
PstNI CAGNNNCTG 2 cut(s) 1667, 2275
PsuI RGATCY 4 cut(s) 1507, 1928, 2871, 3632
PsyI GACNNNGTC 2 cut(s) 631, 1780
PvuI CGATCG 1 cut(s) 135
PvuII CAGCTG 1 cut(s) 3509
Rsr2I CGGWCCG 1 cut(s) 316
RsrII CGGWCCG 1 cut(s) 316
SacI GAGCTC 1 cut(s) 48
SapI GCTCTTC 1 cut(s) 1828
ScaI AGTACT 4 cut(s) 1329, 2151, 2773, 3242
SchI GAGTC 6 cut(s) 50, 89, 304, 733, 3644, 4808
SduI GDGCHC 6 cut(s) 48, 2781, 3112, 3367, 4491, 4573
SexAI ACCWGGT 2 cut(s) 2658, 3933
SfcI CTRYAG 1 cut(s) 3527
SfuI TTCGAA 1 cut(s) 641
SinI GGWCC 9 cut(s) 316, 585, 848, 1230, 1281, 2477, 3571, 4153, 4260
SmaI CCCGGG 1 cut(s) 439
SmlI CTYRAG 4 cut(s) 1211, 3370, 3863, 4681
SmoI CTYRAG 4 cut(s) 1211, 3370, 3863, 4681
SphI GCATGC 1 cut(s) 4736
SseBI AGGCCT 1 cut(s) 3255
SspI AATATT 1 cut(s) 5109
SstI GAGCTC 1 cut(s) 48
StuI AGGCCT 1 cut(s) 3255
StyI CCWWGG 3 cut(s) 39, 2510, 3211
TaiI ACGT 4 cut(s) 1651, 3806, 4660, 5234
TaqII GACCGA 2 cut(s) 247, 364
TauI GCSGC 8 cut(s) 287, 446, 455, 458, 464, 467, 490, 3090
TscAI CASTG 8 cut(s) 802, 1751, 2788, 3649, 4171, 4242, 4496, 4905
TseFI GTSAC 6 cut(s) 1535, 1642, 1963, 2546, 3150, 4387
Tsp45I GTSAC 6 cut(s) 1535, 1642, 1963, 2546, 3150, 4387
TspGWI ACGGA 4 cut(s) 106, 2536, 2551, 4771
TspMI CCCGGG 1 cut(s) 437
TspRI CASTG 8 cut(s) 802, 1751, 2788, 3649, 4171, 4242, 4496, 4905
Tth111I GACNNNGTC 2 cut(s) 631, 1780
VneI GTGCAC 2 cut(s) 2777, 4569
VpaK11BI GGWCC 9 cut(s) 316, 585, 848, 1230, 1281, 2477, 3571, 4153, 4260
VspI ATTAAT 1 cut(s) 4383
XagI CCTNNNNNAGG 1 cut(s) 3863
XbaI TCTAGA 3 cut(s) 1753, 2353, 4097
XceI RCATGY 5 cut(s) 2012, 2950, 3729, 4736, 4740
XmaI CCCGGG 1 cut(s) 437
XmnI GAANNNNTTC 5 cut(s) 1822, 1916, 2523, 3143, 4014
ZrmI AGTACT 4 cut(s) 1329, 2151, 2773, 3242
Zsp2I ATGCAT 2 cut(s) 1030, 4738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.