Rh1CG278300

Vacuolar protein sorting-associated protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0006617
Physical Location & Seq
Forward (+)
54800006 .. 54810122
10117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG278300.1

Sequence Viewer

Length: 5811 bp
ATGACCAAGAAGCTCACACAATCGGAACCACAACTCGCCATGGAGCTCGACCTTGACTCGTTCCTCAACTCACACCTCTCCTCCTCCTCCTCCGACTCCGACCCCGACCTCACCTCCGTCCCGCGCCGTACGATCGACGAAATCCTCAACGACACCGATTCATCCAGGTCAGCATCCCCCTCCTCTTCCCCGCGTAGTTCGACCTTATCTCAGTTCCTCGCGTCCGATCCCAAACCGCCACAGAGCGACGCCGTTTCGGTCGCCAGCGGCAGAACCGAGGGAGCGGGGAGGTTGAGTCAATTGGAGGAGAAGGCGGTCCGGCCGGGTTTGAATTTGTACAGCCGGGCTAGGTCCGGGGATTTCCCGGACGATTCGGTCGGGAGAGTTTCGAGGCCGTCGCCGTGGCTGTTGGGAGGTGTGAGGACGAATGCGAAGCCCGGGGCCGCGCTCGCGGCGGCTGCGGCGGCTTCGAGGTCGATGCCGACGCCCCACGCCGCGGCGATCAAGTCGAGGAGGAGCGCCGGGAGCGGGAGTTTCCAGAAAGCTGTGGAGGCTGCGGAATTGGGTGAGAGATCGGAGGGAGGGTCCAATTTTAACGGGGATAGCAATGCGGTTGGTTCCGAAACGACGCCGTCGAATTCGAATGAAGTAGAGGAGGATTTTGGTGGTGCGTTGGGGAGGAAAGATGAGGTTTTGGAGAGGGAGAGGGAGAGGGAGGTAGAGCAGGTGAGTCAGGTTGATGAAGTTAGTGCTGGCAATGCTGGTGAAGAATTGGATGAGAATTCAACAAATTTAGATGCCACTGATGTTAGAGATAATGAATTGGATGAGAATGTTCGGGTTTCGGAGGAAGGTCCACCAGAGGAAGAAGATTTAGATGAAAATAGTCCTTCTTTGAAACATAGAGATGTTGAGGATGAGCAGCATCTGGGTGGTATTGATGATAATGATGAGGTTGGTGATAATGATAATGATAAAGATGGTGATGATCATTTTGATGATCATGATGATGCATTGGGAACTTCAATCACTCAACTTGTGGAGGAGAGAATCGAGCAACTGGAGAGCAGGAGGATTAGTAAAAAAGCGGAAAAGAAATCAAGAAAGCCACTTGAAATCGCCGAAGAGCTGGAGAAGAAACAAGCTTCAACTGCTTTGGATTGGGAAGAGGGTGCTGCTGCTCAGCCTATGAGACTTGAGGGTGTTCGGAGAGGGTCCACCACGTTGGGATACTTCAATGTTGATGCTAAAAATACCATTACGCGGACCCTTTCTGCTCCGGCTTTGAGGCGTGATCATGGTACGCCGCAAGTCCTGGGAGTTCACAGTAATTACATTGCAATAGGAATGTCAAGAGGGGTTGTCCTTGTTGTCCCCAGTAAATACTCTCCTTATAATGCTGACAACATGGATGCAAAGCTGATATTTCTTGGATTGCAAGGAGAGCGGTCTTATGCTGCGGTGACTTCTATATCCTTCAATCAGCAAGGGGATCTACTCTTAGCTGGATACGCCGATGGTCACATTACAGTTTGGGATGTTCAGAGGGCATCTGCTGCCAAAGTAATTACCGGAGAACATACAGCTCCAGTTGTACATACTTTTTTTTTGGGGCATGATTCTCAGGTCACACGTAATTTTAAGGCAGTTACTGGGGATAGTAAGGGTCTGGTACTGTTACATTCCTTCTCTGTGGTTCCCCTGCTTAATAGATTCTCCATCAAAACACAGTGCCTTCTAGATGGACAAAAAACAGGCACGACGCTGTCAGCTTCACCACTCCTCATTGATGAATTGAGTGGAAGCTCTTCACTTTCCTCTCAGGGAAATGCGATGGGTTCAGGCAGCAGTATTGGTGGTATGATGGGTGGTGTGGTTGGTGGGGATGCAGGTTGGAAACTCTTCAATGAAGGATCCTCTTTGTTTGAAGAAGGTGTGGTCGTGTTTGTCACCCATCAAAATGCTTTGGTGGTAAGACTAACTCCCACTTTGCATGTCTATGCACAACTTTCTAAACCAGATGGGGTTCGGGAGGGTTCCATGCCTTGTACTACTTGGAAATGCACAACTCAATTGCATAATTCACCTACTAATAGTGAGAATACGCCTACTGAAGCAGTTGAAAGAGTTTCATTACTTGCAATTGCTTGGGATCGGAAAGTTCAGGTAGCAAAGTTGGTCAAATCAGAGCTGAAAGTATATGGTAAATGGTCTCTTGAAAGTGCAGCCATAGGTGTGGCTTGGTTGGATGATCAGATGCTGGTGGTTCTCACGATAACTGGACAACTTTGTTTGTTTGCAAAGGATGGAACTGTAATTCACCAAACAAGTTTTTCTCTAGATGGTTTTAGAGGGGATGATCTCATTTCTTATCATACTCACTTCATCAATGTATTTGGAAACCCTGAAAAAGCTTATAACAATTGCATAGCTGTGAGAGGAGCTTCTGTATATGTACTTGGACCTACTCATCTTATTGTTTCCCGGCTTCTCCCATGGAAGGAACGGATTCAGGTTTTACGGAGAGCTGGTGACTGGATGGGTTCTTTGAACATGGCAATGACAATTTATGATGGCCAAGCTCATGGTGTTGTTGACCTTCCTAGAACTCTGGATGCTGTACAGGAGGTCATAATGCCCTACCTGGTTGAGTTGCTTTTGTCATATGTAGAGGAAGTTTTTTCTTATATTTCAGTAGCATTCTGCAACCAAATTGAAAAAATGGATCAAGTTGATGACTTAAACAGACAAAGTAGTACTGTGCACACTGAAATTAAAGAGCAATACACCCGTGTTGGTGGGGTTGCAGTTGAATTCTGTGTCCATATCAAGAGGACTGACATACTTTTTGATGAGATCTTCTTGAAGTTTGTAGCTGTTCAACAGAGAGACACATTTTTGGAACTATTGGAGCCTTATATATTGAAGGACATGCTTGGTTCTCTTCCACCTGAGATTATGCAAGCTCTGGTAGAACATTACAGCCACAAAGGGTGGTTACAGCGTGTTGAGCAATGTGTCCTTCACATGGATATTTCTTCATTGGATTTCAATCAGGTTGTTAGGTTATGCCGCGAGCATGGATTATATAGTGCCCTGGTGTATCTTTTCAACAAAGGATTGAATGATTTCAGGTCACCATTAGAGGAGCTGTTGGTGGTCTTGCGAAATAGTCAAAAAGAAGGTGCTATGGCCCTTGGATACAGGATGCTAGTTTATTTGAAGTACTGCTTTTCAGGCCTTGCTTTTCCACCAGGACAAGGAAAAATTCCTCCTTCACGCTTGCCATCTCTCAGAACAGAACTTTTGCAGTTTTTGTTGGAGGGTTCTGATGCCCCTAATTCAAGAGCACTCTCAAGCGAATTCCCAGGAGGAGAATATCTCAACCTTTATTATCTCTTAGAACTAGATACTGAAGCTACTTTAGATGTTCTGAGATGTGCTTTCACAGAAGTTGAAATCTCGAAATGGGACTTCTCTTCTCAAAATTCAGCTGATGCAGACACAGAGCTGCAGTATGGGAATAATTCAACGGCTCAAAGTCAGGATTCATTGGTCCAGAATACAATTGATACTCTTATTCACATTATTAGTAAGGACATTCCCCAGAAGGATGGATCTGCTAGCAGTGTTGACTCTGGATCAGTTGTAGAGTGGCCTTCAAAGAAAGATATTGATCATCTATTCGAGTTTATTGCATATTATGTTGCATGTGGAAAAGCTAATGTCTCGAAGACTGTACTTTCTCAGATTTTGGAGTATTTGACATCACAGAATAACTTTCCAACGTCTGTTTCTGGGGATAGCATAATTTCAAAAAGAAGAGAGAAGCAGGTTCTTGGCCTTCTTGAGGTAGTGCCGGAGACTGATTGGAATTCATCTTCTGTGTTACAGCTATGTGAGAAAGCACAATTTTACCAGGTATGTGGCCTAATTCATACCAGCAGACATCAGTATATTGCTGCCCTGGATTGCTATATGAAAGATGCAGCCGAACCCATTCATGCCTTTGCCTTTATCAACAAAACATTGTTACAGTTGACTGACGATGAATGTGCTGCTTTTCAATCAGCAGTTATTTCTAGAATTCCAGAGCTATTTAACTTAAACAGAGAGGGTGCATTCTTCTTGGTCATGGACCATTTTACCAGTGCAGAAGGTTCTCATATCCTATCTGAACTTCGCTCACATCCACGAAGCCTTTTCCTGTACTTAAAAACAGTGATTGAGGTTCATTTATCTGGCACCCTCGACTTCTCAAGTTTAAAAACCGACAATCTTATGGGAGTAAAGGATCAAACAAAAGGAGTTGAGGCTTTCTTAGAAAGGGTCTCTAATTTCCCACAGCTTTTGCGTGATAGTCCTATTAATGTGACTGATGATATGATTGAGCTTTATCTGGAGCTGTTATGCCAGTTTGAATGCAAATCAGTTCTCAAATTCCTAGAGACATTTGATAGCTATCGTGTGGAGCACTGTTTACGCCTCTGCCAGAAATATGCAATAATAGATGCTTCATCATTCTTGTTGGAGAGGGTTGGTGATGTTGGAAGTGCACTTTTACTTACACTTTCCACCCTCAATGACAAATTTATCAAGCTTGAAACTGCTGTTGGAAGTCTAAATTTAAGTGGGGCTTCACGTGGTTCTGCTAGTACAGAATGCTTGAGCAATGCTTTGAAAGTGGAGGAGGTGAATGATATAGACAGTATATTGCATGCATGTATAGGATTGTGCCAACGGAACACCCATCGTCTAAATCCTGATGAATCAGAGGCACTCTGGTTCAAATTGCTTGACTCGTTTTGTGAGCCTTTAATGGATTCGTTTAGTGCTGGTACAGTATCTAAAGGAGAAGATCATAACAGAATGGTAACCAATTCATTGGATTCACAAGAGGATGAAATGAATTTCACAATCAAGTGGAGAATCCCAAAGGCTCATAAAGGTGCTGATATTTTAAGAAAATTGTTTTCTCGGTTCATCAAGGAGATTGTTGAGGGCATGATAGGATATGTTCGCCTTCCAACTATCATGTCAAAGCTCCTTTCTGACAATGGTAGCCAGGAATTTGGTGATTTTAAATTCACAATATTGGGGATGCTTAGCACATATGGCTTTGAAAGAAGAATTCTGGATACTGCCAAGTCCCTGATAGAAGATGATACATTCTATACTATGAGCATCCTCAAGAAAGGAGCCTCACATGGGTATGCTCCTCGCAACCCAAAATGTTGCATATGCGATTGCCTCCTTGACAAGAACTCCTCCAGCTACATTCGCATTTTCAATTGTGGTCATGCAACACATCTTAAATGCGAAGTTTCAGAGAATGAAACATCAAGTAGTAGCTCGTCCTCTGGATGCCCAGTTTGTATGCCCAAGAAGAAATCTCAGAGGTCTAAAAACAAATCGGTTCTAGCAGAGGAGAGTTTAGTGAAGGTGTTTTCATCAAGAACCCATCATAGTCATGGAACAGTTGTTCACCCACATGAAAGCACTTCTGAGAATAGCTATGGGATTCAGCAGATATCACGATTTGAGATGTTGACCAATTTGCAGAAACATCGTGGATTAGTCGAGATTGAAAACATGCCTCAATTGAGGCTTGCCCCACCAGCTGTGTACCATGAAAAGGTTAAGCACGGACCTGTTCTGTCACCAGGAGAGAGCAGCAGTGATCTTGTGAGAACAGAGAAACAAAGTAAAATCAAGCAACTCAGGGAGGTAAAAGTAAAAGGATCATCTCTTCGGTTTCCCTTGAAAACAAACTTATTTGGTAATGGCAAGGACAAGATCAGCAGGCGATGA

Protein Analysis

1936

Amino Acids

213.85

Weight (kDa)

5.34

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_VPS8 PF23410 418 - 602 9.6e-23 Vps8 beta-propeller
VPS8_N PF23306 419 - 592 1.7e-06 VPS8-like, N-terminal beta propeller repeats
Beta-prop_WDR36-Utp21_1st PF25171 446 - 538 6.9e-06 WDR36/Utp21 first beta-propeller
TPR_Vps8 PF12816 966 - 1149 5.8e-51 Vps8 central TPR
TPR_Vps41 PF23556 1461 - 1529 1.2e-06 Vps41 TPR-like region
TPR_VPS8_2 PF25066 1581 - 1728 2.1e-06 VPS8-like, TPR-like repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1395, 2418
AarI CACCTGC 1 cut(s) 715
AasI GACNNNNNNGTC 1 cut(s) 374
Acc36I ACCTGC 3 cut(s) 715, 1880, 3823
AccB1I GGYRCC 1 cut(s) 4244
AccBSI CCGCTC 3 cut(s) 284, 528, 1447
AccII CGCG 8 cut(s) 124, 193, 221, 446, 452, 497, 1264, 3075
AcoI YGGCCR 2 cut(s) 320, 2575
AcuI CTGAAG 2 cut(s) 2133, 3435
AcvI CACGTG 1 cut(s) 4643
AcyI GRCGYC 3 cut(s) 249, 485, 629
AfiI CCNNNNNNNGG 9 cut(s) 496, 528, 1263, 2500, 3028, 3260, 3850, 5208, 5635
AflIII ACRYGT 1 cut(s) 1631
AjuI GAANNNNNNNTTGG 4 cut(s) 3778, 3810, 4596, 4628
AloI GAACNNNNNNTCC 2 cut(s) 5466, 5498
Alw21I GWGCWC 5 cut(s) 48, 2766, 3352, 4476, 4558
Alw26I GTCTC 7 cut(s) 1186, 2217, 2883, 3733, 3857, 4336, 4442
Alw44I GTGCAC 2 cut(s) 2762, 4554
AlwNI CAGNNNCTG 3 cut(s) 928, 1652, 2260
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 9 cut(s) 320, 392, 441, 2575, 3192, 3238, 3656, 3841, 3928
ApaLI GTGCAC 2 cut(s) 2762, 4554
ArsI GACNNNNNNTTYG 2 cut(s) 2831, 2863
AseI ATTAAT 1 cut(s) 4368
Asp700I GAANNNNTTC 5 cut(s) 1807, 1901, 2508, 3128, 3999
AspLEI GCGC 3 cut(s) 126, 448, 521
AsuC2I CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2485
AsuII TTCGAA 1 cut(s) 641
AsuNHI GCTAGC 1 cut(s) 3623
AvaI CYCGRG 1 cut(s) 437
BaeGI GKGCMC 3 cut(s) 2766, 3097, 4558
BalI TGGCCA 1 cut(s) 2577
BamHI GGATCC 1 cut(s) 1913
BanI GGYRCC 1 cut(s) 4244
BanII GRGCYC 1 cut(s) 48
BbrPI CACGTG 1 cut(s) 4643
BbsI GAAGAC 1 cut(s) 3740
Bbv12I GWGCWC 5 cut(s) 48, 2766, 3352, 4476, 4558
BceAI ACGGC 6 cut(s) 111, 236, 379, 385, 616, 3549
BcgI CGANNNNNNTGC 8 cut(s) 460, 494, 3677, 3711, 4037, 4071, 5549, 5583
BciVI GTATCC 4 cut(s) 1223, 1502, 3194, 5131
BclI TGATCA 5 cut(s) 988, 1000, 1294, 2251, 3676
BcnI CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2485
BcoDI GTCTC 7 cut(s) 1186, 2217, 2883, 3733, 3857, 4336, 4442
BfmI CTRYAG 1 cut(s) 3512
BfoI RGCGCY 1 cut(s) 522
BfuAI ACCTGC 3 cut(s) 715, 1880, 3823
BfuI GTATCC 4 cut(s) 1223, 1502, 3194, 5131
BglII AGATCT 1 cut(s) 2856
BlpI GCTNAGC 2 cut(s) 1182, 5105
BmcAI AGTACT 2 cut(s) 2758, 3227
BmeT110I CYCGRG 1 cut(s) 437
BmrI ACTGGG 3 cut(s) 1371, 1662, 5363
BmtI GCTAGC 1 cut(s) 3627
BmuI ACTGGG 3 cut(s) 1371, 1662, 5363
BpiI GAAGAC 1 cut(s) 3740
BplI GAGNNNNNCTC 2 cut(s) 3366, 3398
BpmI CTGGAG 5 cut(s) 1082, 1151, 1572, 4421, 5254
Bpu1102I GCTNAGC 2 cut(s) 1182, 5105
Bpu14I TTCGAA 1 cut(s) 641
BpuEI CTTGAG 6 cut(s) 1217, 3340, 3869, 4243, 4687, 5174
BpuMI CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2485
BsaAI YACGTR 2 cut(s) 1634, 4643
BsaBI GATNNNNATC 3 cut(s) 3051, 3675, 4461
BsaHI GRCGYC 3 cut(s) 249, 485, 629
BsaI GGTCTC 2 cut(s) 2217, 4336
BsaWI WCCGGW 1 cut(s) 1571
Bsc4I CCNNNNNNNGG 9 cut(s) 496, 528, 1263, 2500, 3028, 3260, 3850, 5208, 5635
Bse1I ACTGG 9 cut(s) 1065, 1377, 1589, 1657, 2284, 2540, 4149, 4414, 5369
Bse3DI GCAATG 6 cut(s) 613, 763, 1335, 2565, 3020, 4678
Bse8I GATNNNNATC 3 cut(s) 3051, 3675, 4461
BseJI GATNNNNATC 3 cut(s) 3051, 3675, 4461
BseLI CCNNNNNNNGG 9 cut(s) 496, 528, 1263, 2500, 3028, 3260, 3850, 5208, 5635
BseMI GCAATG 6 cut(s) 613, 763, 1335, 2565, 3020, 4678
BseNI ACTGG 9 cut(s) 1065, 1377, 1589, 1657, 2284, 2540, 4149, 4414, 5369
BseSI GKGCMC 3 cut(s) 2766, 3097, 4558
BseX3I CGGCCG 1 cut(s) 320
BsgI GTGCAG 2 cut(s) 2244, 4173
Bsh1236I CGCG 8 cut(s) 124, 193, 221, 446, 452, 497, 1264, 3075
Bsh1285I CGRYCG 4 cut(s) 135, 261, 323, 378
BshFI GGCC 9 cut(s) 322, 394, 443, 2577, 3194, 3240, 3658, 3843, 3930
BshNI GGYRCC 1 cut(s) 4244
BsiEI CGRYCG 4 cut(s) 135, 261, 323, 378
BsiHKAI GWGCWC 5 cut(s) 48, 2766, 3352, 4476, 4558
BsiHKCI CYCGRG 1 cut(s) 437
BsiWI CGTACG 1 cut(s) 128
BslFI GGGAC 4 cut(s) 104, 1358, 3485, 5134
BslI CCNNNNNNNGG 9 cut(s) 496, 528, 1263, 2500, 3028, 3260, 3850, 5208, 5635
BsmAI GTCTC 7 cut(s) 1186, 2217, 2883, 3733, 3857, 4336, 4442
BsmFI GGGAC 4 cut(s) 104, 1358, 3485, 5134
BsmI GAATGC 5 cut(s) 433, 2699, 4121, 4427, 4667
BsnI GGCC 9 cut(s) 322, 394, 443, 2577, 3194, 3240, 3658, 3843, 3930
Bso31I GGTCTC 2 cut(s) 2217, 4336
BsoBI CYCGRG 1 cut(s) 437
Bsp119I TTCGAA 1 cut(s) 641
Bsp1286I GDGCHC 6 cut(s) 48, 2766, 3097, 3352, 4476, 4558
Bsp1407I TGTACA 3 cut(s) 336, 1594, 2620
Bsp1720I GCTNAGC 2 cut(s) 1182, 5105
Bsp19I CCATGG 2 cut(s) 39, 2495
BspANI GGCC 9 cut(s) 322, 394, 443, 2577, 3194, 3240, 3658, 3843, 3930
BspFNI CGCG 8 cut(s) 124, 193, 221, 446, 452, 497, 1264, 3075
BspHI TCATGA 1 cut(s) 1003
BspMAI CTGCAG 1 cut(s) 3516
BspMI ACCTGC 3 cut(s) 715, 1880, 3823
BspOI GCTAGC 1 cut(s) 3627
BspQI GCTCTTC 2 cut(s) 1119, 1813
BspT104I TTCGAA 1 cut(s) 641
BspT107I GGYRCC 1 cut(s) 4244
BspTNI GGTCTC 2 cut(s) 2217, 4336
BsrBI CCGCTC 3 cut(s) 284, 528, 1447
BsrDI GCAATG 6 cut(s) 613, 763, 1335, 2565, 3020, 4678
BsrGI TGTACA 3 cut(s) 336, 1594, 2620
BsrI ACTGG 9 cut(s) 1065, 1377, 1589, 1657, 2284, 2540, 4149, 4414, 5369
BssNI GRCGYC 3 cut(s) 249, 485, 629
BssT1I CCWWGG 3 cut(s) 39, 2495, 3196
Bst6I CTCTTC 9 cut(s) 190, 1119, 1161, 1813, 1907, 2949, 3484, 3817, 5754
BstACI GRCGYC 3 cut(s) 249, 485, 629
BstAPI GCANNNNNTGC 1 cut(s) 1556
BstAUI TGTACA 3 cut(s) 336, 1594, 2620
BstBAI YACGTR 2 cut(s) 1634, 4643
BstBI TTCGAA 1 cut(s) 641
BstDSI CCRYGG 4 cut(s) 39, 401, 495, 2495
BstEII GGTNACC 2 cut(s) 3135, 4873
BstENI CCTNNNNNAGG 1 cut(s) 3848
BstFNI CGCG 8 cut(s) 124, 193, 221, 446, 452, 497, 1264, 3075
BstH2I RGCGCY 1 cut(s) 522
BstHHI GCGC 3 cut(s) 126, 448, 521
BstMAI GTCTC 7 cut(s) 1186, 2217, 2883, 3733, 3857, 4336, 4442
BstMCI CGRYCG 4 cut(s) 135, 261, 323, 378
BstNSI RCATGY 6 cut(s) 1997, 2935, 3714, 4721, 4725, 5596
BstPI GGTNACC 2 cut(s) 3135, 4873
BstSFI CTRYAG 1 cut(s) 3512
BstSLI GKGCMC 3 cut(s) 2766, 3097, 4558
BstUI CGCG 8 cut(s) 124, 193, 221, 446, 452, 497, 1264, 3075
BstV2I GAAGAC 1 cut(s) 3740
BstX2I RGATCY 4 cut(s) 1492, 1913, 2856, 3617
BstXI CCANNNNNNTGG 7 cut(s) 1225, 2236, 2585, 3614, 3926, 4885, 5072
BstYI RGATCY 4 cut(s) 1492, 1913, 2856, 3617
BstZI CGGCCG 1 cut(s) 320
BsuI GTATCC 4 cut(s) 1223, 1502, 3194, 5131
BsuRI GGCC 9 cut(s) 322, 394, 443, 2577, 3194, 3240, 3658, 3843, 3930
BtgI CCRYGG 4 cut(s) 39, 401, 495, 2495
BtgZI GCGATG 1 cut(s) 1847
BtsI GCAGTG 2 cut(s) 3634, 5683
BtsIMutI CAGTG 8 cut(s) 801, 1736, 2766, 3634, 4156, 4227, 4474, 5683
BveI ACCTGC 3 cut(s) 715, 1880, 3823
CaiI CAGNNNCTG 3 cut(s) 928, 1652, 2260
CciI TCATGA 1 cut(s) 1003
CfoI GCGC 3 cut(s) 126, 448, 521
Cfr42I CCGCGG 1 cut(s) 498
Cfr9I CCCGGG 1 cut(s) 437
CpoI CGGWCCG 1 cut(s) 316
CseI GACGC 5 cut(s) 210, 257, 493, 637, 1771
CsiI ACCWGGT 2 cut(s) 2643, 3918
CspCI CAANNNNNGTGG 2 cut(s) 4332, 4367
CspI CGGWCCG 1 cut(s) 316
DraI TTTAAA 2 cut(s) 4266, 5083
DrdI GACNNNNNNGTC 1 cut(s) 374
DseDI GACNNNNNNGTC 1 cut(s) 374
EaeI YGGCCR 2 cut(s) 320, 2575
EagI CGGCCG 1 cut(s) 320
Eam1104I CTCTTC 9 cut(s) 190, 1119, 1161, 1813, 1907, 2949, 3484, 3817, 5754
EarI CTCTTC 9 cut(s) 190, 1119, 1161, 1813, 1907, 2949, 3484, 3817, 5754
Ecl136II GAGCTC 1 cut(s) 46
EclXI CGGCCG 1 cut(s) 320
Eco130I CCWWGG 3 cut(s) 39, 2495, 3196
Eco147I AGGCCT 1 cut(s) 3240
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 2 cut(s) 2217, 4336
Eco32I GATATC 1 cut(s) 5532
Eco52I CGGCCG 1 cut(s) 320
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 2133, 3435
Eco72I CACGTG 1 cut(s) 4643
Eco88I CYCGRG 1 cut(s) 437
Eco91I GGTNACC 2 cut(s) 3135, 4873
EcoICRI GAGCTC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 3848
EcoO65I GGTNACC 2 cut(s) 3135, 4873
EcoRI GAATTC 7 cut(s) 637, 781, 2813, 3362, 3874, 4086, 5130
EcoRV GATATC 1 cut(s) 5532
EcoT14I CCWWGG 3 cut(s) 39, 2495, 3196
EcoT22I ATGCAT 2 cut(s) 1015, 4723
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 3 cut(s) 39, 2495, 3196
FalI AAGNNNNNCTT 4 cut(s) 3215, 3247, 4621, 4653
FaqI GGGAC 4 cut(s) 104, 1358, 3485, 5134
FauI CCCGC 4 cut(s) 129, 198, 277, 521
FauNDI CATATG 3 cut(s) 2665, 5113, 5240
FbaI TGATCA 5 cut(s) 988, 1000, 1294, 2251, 3676
FriOI GRGCYC 1 cut(s) 48
GlaI GCGC 3 cut(s) 125, 447, 520
GsuI CTGGAG 5 cut(s) 1082, 1151, 1572, 4421, 5254
HaeII RGCGCY 1 cut(s) 522
HaeIII GGCC 9 cut(s) 322, 394, 443, 2577, 3194, 3240, 3658, 3843, 3930
HgaI GACGC 5 cut(s) 210, 257, 493, 637, 1771
HhaI GCGC 3 cut(s) 126, 448, 521
Hin1I GRCGYC 3 cut(s) 249, 485, 629
Hin6I GCGC 3 cut(s) 124, 446, 519
HinP1I GCGC 3 cut(s) 124, 446, 519
HincII GTYRAC 4 cut(s) 2596, 3634, 4041, 5550
HindII GTYRAC 4 cut(s) 2596, 3634, 4041, 5550
HindIII AAGCTT 3 cut(s) 1143, 2412, 4598
Hpy99I CGWCG 7 cut(s) 140, 251, 400, 487, 631, 637, 1765
HpyCH4IV ACGT 4 cut(s) 1223, 1633, 3788, 4642
HpySE526I ACGT 4 cut(s) 1223, 1633, 3788, 4642
Hsp92I GRCGYC 3 cut(s) 249, 485, 629
HspAI GCGC 3 cut(s) 124, 446, 519
Ksp22I TGATCA 5 cut(s) 988, 1000, 1294, 2251, 3676
KspI CCGCGG 1 cut(s) 498
LguI GCTCTTC 2 cut(s) 1119, 1813
MabI ACCWGGT 2 cut(s) 2643, 3918
MaeII ACGT 4 cut(s) 1223, 1633, 3788, 4642
MbiI CCGCTC 3 cut(s) 284, 528, 1447
MfeI CAATTG 7 cut(s) 299, 2072, 2142, 2422, 3567, 5290, 5600
MflI RGATCY 4 cut(s) 1492, 1913, 2856, 3617
MhlI GDGCHC 6 cut(s) 48, 2766, 3097, 3352, 4476, 4558
MlsI TGGCCA 1 cut(s) 2577
MluNI TGGCCA 1 cut(s) 2577
MlyI GAGTC 6 cut(s) 50, 89, 304, 739, 3629, 4793
Mox20I TGGCCA 1 cut(s) 2577
Mph1103I ATGCAT 2 cut(s) 1015, 4723
MroXI GAANNNNTTC 5 cut(s) 1807, 1901, 2508, 3128, 3999
MscI TGGCCA 1 cut(s) 2577
Msp20I TGGCCA 1 cut(s) 2577
MspA1I CMGCKG 4 cut(s) 267, 497, 3494, 5621
MunI CAATTG 7 cut(s) 299, 2072, 2142, 2422, 3567, 5290, 5600
Mva1269I GAATGC 5 cut(s) 433, 2699, 4121, 4427, 4667
MvnI CGCG 8 cut(s) 124, 193, 221, 446, 452, 497, 1264, 3075
NciI CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2485
NcoI CCATGG 2 cut(s) 39, 2495
NdeI CATATG 3 cut(s) 2665, 5113, 5240
NheI GCTAGC 1 cut(s) 3623
NmuCI GTSAC 8 cut(s) 1462, 1520, 1627, 1948, 2531, 3135, 4372, 5658
NsiI ATGCAT 2 cut(s) 1015, 4723
NspI RCATGY 6 cut(s) 1997, 2935, 3714, 4721, 4725, 5596
NspV TTCGAA 1 cut(s) 641
PaeI GCATGC 1 cut(s) 4721
PagI TCATGA 1 cut(s) 1003
PaqCI CACCTGC 1 cut(s) 715
PceI AGGCCT 1 cut(s) 3240
PciSI GCTCTTC 2 cut(s) 1119, 1813
PcsI WCGNNNNNNNCGW 1 cut(s) 632
PctI GAATGC 5 cut(s) 433, 2699, 4121, 4427, 4667
PdmI GAANNNNTTC 5 cut(s) 1807, 1901, 2508, 3128, 3999
Pfl23II CGTACG 1 cut(s) 128
PflFI GACNNNGTC 2 cut(s) 631, 1765
PfoI TCCNGGA 1 cut(s) 363
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 6 cut(s) 50, 89, 303, 738, 3629, 4793
PmaCI CACGTG 1 cut(s) 4643
PmlI CACGTG 1 cut(s) 4643
PpsI GAGTC 6 cut(s) 50, 89, 303, 738, 3629, 4793
Ppu21I YACGTR 2 cut(s) 1634, 4643
PshBI ATTAAT 1 cut(s) 4368
PsiI TTATAA 2 cut(s) 1395, 2418
Psp124BI GAGCTC 1 cut(s) 48
PspCI CACGTG 1 cut(s) 4643
PspEI GGTNACC 2 cut(s) 3135, 4873
PspLI CGTACG 1 cut(s) 128
PstI CTGCAG 1 cut(s) 3516
PstNI CAGNNNCTG 3 cut(s) 928, 1652, 2260
PsuI RGATCY 4 cut(s) 1492, 1913, 2856, 3617
PsyI GACNNNGTC 2 cut(s) 631, 1765
PvuI CGATCG 1 cut(s) 135
PvuII CAGCTG 2 cut(s) 3494, 5621
Rsr2I CGGWCCG 1 cut(s) 316
RsrII CGGWCCG 1 cut(s) 316
SacI GAGCTC 1 cut(s) 48
SacII CCGCGG 1 cut(s) 498
SapI GCTCTTC 2 cut(s) 1119, 1813
ScaI AGTACT 2 cut(s) 2758, 3227
SchI GAGTC 6 cut(s) 50, 89, 304, 739, 3629, 4793
SduI GDGCHC 6 cut(s) 48, 2766, 3097, 3352, 4476, 4558
SexAI ACCWGGT 2 cut(s) 2643, 3918
SfcI CTRYAG 1 cut(s) 3512
Sfr303I CCGCGG 1 cut(s) 498
SfuI TTCGAA 1 cut(s) 641
SgrBI CCGCGG 1 cut(s) 498
SmaI CCCGGG 1 cut(s) 439
SmlI CTYRAG 6 cut(s) 1196, 3355, 3848, 4258, 4666, 5189
SmoI CTYRAG 6 cut(s) 1196, 3355, 3848, 4258, 4666, 5189
SphI GCATGC 1 cut(s) 4721
SseBI AGGCCT 1 cut(s) 3240
SspI AATATT 1 cut(s) 5094
SstI GAGCTC 1 cut(s) 48
StuI AGGCCT 1 cut(s) 3240
StyI CCWWGG 3 cut(s) 39, 2495, 3196
TaiI ACGT 4 cut(s) 1226, 1636, 3791, 4645
TaqII GACCGA 2 cut(s) 247, 364
TscAI CASTG 8 cut(s) 808, 1736, 2773, 3634, 4156, 4227, 4481, 5683
TseFI GTSAC 8 cut(s) 1462, 1520, 1627, 1948, 2531, 3135, 4372, 5658
Tsp45I GTSAC 8 cut(s) 1462, 1520, 1627, 1948, 2531, 3135, 4372, 5658
TspGWI ACGGA 5 cut(s) 106, 2521, 2536, 4756, 5661
TspMI CCCGGG 1 cut(s) 437
TspRI CASTG 8 cut(s) 808, 1736, 2773, 3634, 4156, 4227, 4481, 5683
Tth111I GACNNNGTC 2 cut(s) 631, 1765
VneI GTGCAC 2 cut(s) 2762, 4554
VspI ATTAAT 1 cut(s) 4368
XagI CCTNNNNNAGG 1 cut(s) 3848
XbaI TCTAGA 3 cut(s) 1738, 2338, 4082
XceI RCATGY 6 cut(s) 1997, 2935, 3714, 4721, 4725, 5596
XcmI CCANNNNNNNNNTGG 1 cut(s) 5468
XmaI CCCGGG 1 cut(s) 437
XmnI GAANNNNTTC 5 cut(s) 1807, 1901, 2508, 3128, 3999
ZrmI AGTACT 2 cut(s) 2758, 3227
Zsp2I ATGCAT 2 cut(s) 1015, 4723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.