Rroxscaffold_4G00295450

Vacuolar protein sorting-associated protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006617
Physical Location & Seq
Reverse (-)
15488779 .. 15498728
9950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00295450.1

Sequence Viewer

Length: 5901 bp
ATGCGCATCGTGGTTTTGATTCACAAGAAACAGACTTATACTGACGGAGACGAACTACCGGCCATAGCTAGAAGAAGAAGAAGAAGCAGCAGCAGCAGCAGTAAGAGAGCTTTGGAGATGACCAAGAAGCTTACACAATCGGAACCACAACTCGCCATGGAGCTCGACCTCGACTCGTTCCTCAACTCACACCTTTCCTCCTCCTCCTCCGACTCCGACCCCGACCTCACCTCCGTCCCTCGCCGTACGATCGACGAAATCCTCAACGACACCGATTCATCCAGGTCAGCATCTCCCTCCTCTTCCCCGCGTAGTTCGACCTTATCTCAGTTCCTCGCGTCCGATCCCAAACCGCCACAGAGCGACGCCGTTTCGGTCGCCAGCGGCGGAACCGAGGGAGCGGGGAGGTTGACTCAATTGGAGGAGAAGGCGGTCCGGCCGGGTTTGAATTTGTACAGCCGGGCTAGGTCCGGGGATTTACCGGACGATTCGGTCGGGAGAGTTTCGAGGCCGTCGCCGTGGCTGTTGGGAGGTGTGAGGACGAATGCGAAGCCCGGGGCCGCGCTCGCGGCGGCTGCGGCGGCTTCGAGGTCGATGCCGACGCCGCACGCCGCGGCGATCAAGTCGAGGAGGAGCGCCGGGAGCGGGAGTTTCCAGAAAGCTGTGGAGGCTGCGGAATTGGGTGAGAGATCGGAGGGAGGGTCCAATTTTAACGAGGATAGCAATGCGGTTGGTTCCGAAACGACGCCGTCGAATTCGAATGAAGTAGAGGAGGATTTTGGTGGTGAGTTGGGGAGGAAAGATGAGGTTTTGGAGAGGGAGAGGGAGGTAGAGCAGGTGAGTCAGGTTGATGAAGTTAGTGCTGGCAATGCTGGTGAAGAATTGGATGAGAATTCAACAAATTTAGATGCCACTGATGTTAAAGATAATGAATTGGATGAGAATGTTCGGGTTTCGGAGGAAGGTCCACCAGAGGAAGAAGATTTAGATGAAAATAGTCCTTCCTCGAAACATAGAGATGTTGAGGATGAGCAGCATCTTGGTGGTATTGATGATCATGATGAGGTTGGTGATAATGATAATGATAAAGATGGTGATGATCATTTTGATGATGATGATGATGCATTGGGAACTTCAATCACTCAACTTGTGGAGGAGAGAATCGAGCAACTGGAGAGCAGGAGGATTAGTAAAAAAGCGGAAAAGAAATCAAGAAAGCCACTTGAAATCGCCGAAGAGCTGGAGAAGAAACAAGCTTCAACTGCTTTGGATTGGGAAGAGGGTGCTGCTGCTCAACCTATGAGACTTGAGGGTGTTCGGAGAGGGTCCACCACATTGGGATACTTCAATGTTGATGCTAAAAATACCATTACGCGGACCCTTTCTGCTCCGGCTTTGAGGCGTGATCATGGTACGCCACAAGTCCTGGGAGTTCACAGTAATTACATTGCAATAGGAATGTCAAGAGGGGTTGTCCTTGTTGTCCCCAGTAAATACTCTCCTTATAATGCTGACAACATGGATGCAAAGCTGATATTTCTTGGATTGCAAGGAGAGCGGTCTTATGCTGCGGTAACTTCTATATCCTTCAATCAGCAAGGGGATCTACTCTTAGCTGGATACGCCGACGGTCACATTACAGTTTGGGATGTTCAGAGGGCATCCGCTGCCAAAGTAATTACCGGAGAACATACAGCTCCAGTTGTACATACTTTTTTTTTGGGGCATGATTCTCAGGTCACACGTAATTTTAAGGCAGTTACTGGGGATAGTAAGGGTCTGGTACTGTTACATTCCTTCTCTGTGGTTCCCCTGCTTAATAGATTCTCCATCAAAACACAGTGCCTTCTAGATGGACAAAAAACAGGCACGACGCTGTCAGCTTCACCACTCCTCATTGATGAATTGAGTGGAAGCTCTTCACTTTCCTCTCAGGGAAATGCGATGGGTTCAGGCAGCAGTATTGGTGGTATGATGGGTGGTGTGGTTGGTGGGGATGCAGGTTGGAAACTCTTCAATGAAGGATCCTCTTTGTTTGAAGAAGGTGTGGTCGTGTTTGTCACCCATCAAAATGCTTTGGTGGTAAGACTAACTCCCACTTTGCATGTCTATGCACAACTTTCTAAACCAGATGGGGTTCGGGAGGGTTCCATGCCTTGTACTGCTTGGAAATGCACAACTCAATTGCACAATTCACCTACTAATAGTGAGAATACGCCTACTGAAGCAGTTGAAAGAGTTTCATTACTTGCAATTGCTTGGGATCGGAAAGTTCAGGTAGCAAAGTTGGTCAAATCAGAGCTGAAAGTATATGGTAAATGGTCTCTTGAAAGTGCAGCCATAGGTGTGGCTTGGTTGGATGATCAGATGCTGGTGGTTCTCACAATAACTGGACAACTTTGTTTGTTTGCAAAGGATGGAACTGTAATTCATCAAACAAGTTTTTCTCTAGATGGTTTTAGAGGGGATGATCTCATATCTTATCATACTCACTTCATCAATGTATTTGGAAACCCTGAAAAAGCTTATAACAATTGCATAGCTGTAAGAGGAGCTTCTGTATATGTACTTGGACCTACTCATCTTATTGTTTCCCGGCTTCTCCCGTGGAAGGAACGGATTCAGGTTTTACGGAGAGCTGGTGACTGGATGGGTTCTTTGAACATGGCAATGACAATTTATGATGGCCAAGCTCATGGTGTTGTTGACCTTCCTAGAACTCTGGATGCTGTACAGGAGGTCATAATGCCCTACCTGGTTGAGTTGCTTTTGTCATATGTAGAGGAAGTTTTTTCTTATATTTCAGTAGCATTCTGCAACCAAATTGAAAAAATGGATCAAGTTGATGACTTAAACAGACAAAGTAGTACTGTGCACACTGAAATTAAAGAGCAATACACCCGTGTTGGTGGGGTTGCAGTTGAATTCTGTGTCCATATCAAGAGGACTGACATACTTTTTGATGAGATCTTCTTGAAGTTTGTAGCTGTTCAACAGAGAGACACATTTTTGGAACTATTGGAGCCTTATATATTGAAGGACATGCTTGGTTCTCTTCCACCTGAGATTATGCAAGCTCTGGTAGAACATTACAGCCACAAAGGGTGGTTACAGCGTGTTGAGCAATGTGTCCTTCACATGGATATTTCTTCATTGGATTTCAATCAGGTTGTTAGGTTATGCCGCGAGCATGGATTATATAGTGCCCTGGTGTATCTTTTCAACAAAGGATTGAATGATTTCAGGTCACCATTAGAGGAGCTGTTGGTGGTCTTGCGAAATAGTCAAAAAGAAGGTGCTATGGCCCTTGGATACAGGATGCTAGTTTATTTGAAGTACTGCTTTTCAGGCCTTGCTTTTCCACCAGGACAAGGAAAAATTCCTCCTTCACGCTTGCCATCTCTCAGAACAGAACTTTTGCAGTTTTTGTTGGAGGGTTCTGATGCCCCTAATTCAAGAGCACTCTCAAGCGAATTCCCAGGAGGAGAATATCTCAACCTTTATTATCTCTTAGAACTAGATACTGAAGCTACTTTAGATGTTCTGAGATGTGCTTTCACAGAAGTTGAAATCTTGAAATGGGACTTCTCTTCTCAAAATTCAGCTGATGCAGATACAGAGCTGCAGTATGGGAATAATTCAACGGCTCAAAGTCAGGATTCATTGGTCCAGAATACAATTGATACTCTTATTCACATTATTAGTAAGGACATTCCCCAGAAGGATGGATCTGCTAGCAGTGTTGACTCTGGATCAGTCGTAGAGTGGCCGTCAAAGAAAGATATTGATCATCTATTCGAGTTTATTGCATATTATGTTGCATGTGGAAAAGCTAATGTCTCGAAGACTGTACTTTCTCAGATTTTGGAGTATTTGACATCAGAGAATAACTTTCCAACGTCTGGTTCTGGAGATAGCATAATTTCAAAAAGAAGAGAGAAGCAGGTTCTTGGCCTTCTTGAGGTAGTGCCGGAGACTGATTGGAATTCATCTTCTGTGTTACAGCTATGTGAGAAAGCACAATTTTACCAGGTATGTGGCCTAATTCATACCAGCAGACATCAGTATATTGCTGCCCTGGATTGCTATATGAAAGATGCAGCTGAACCCATTCATGCCTTTGCCTTTATCAACAAAACATTGTTACAGTTGACTGACGATGAATGTGCTGCTTTTCGATCAGCAGTTATTTCTAGAATTCCAGAGCTATTTAACTTAAACAGAGAGGGTGCATTCTTCTTGGTCATGGACCATTTTACCAACGCAGAAGGTTCTCATATCCTATCTGAACTTCGCTCCCATCCACGAAGCCTTTTCCTGTACTTAAAAACAGTGATTGAGGTTCATTTATCTGGCACCCTCGACTTCTCAAGTTTAAAAACCGACAATCTTATGGGAGTAAAGGATCAAACAAAAGGAGTTGAGGCTTTCTTGGAAAGGGTCTCTAATTTCCCACAGCTTTTGCGTGATAGTCCTATTAATGTGACTGATGATATGATTGAGCTTTATCTGGAGCTGTTATGCCAGTTTGAATGCAAATCAGTTCTCAAATTCCTAGAGACATTTGATAGCTATCGCGTGGAGCACTGTTTACGCCTCTGCCAGAAATATGCAATAATAGATGCTTCATCATTCTTGTTGGAGAGGGTTGGTGATGTTGGAAGTGCACTTTTACTTACACTTTCCACCCTCAATGACAAATTTATCAAGCTTGAAACTGCTGTTGGAAGTCTAAATTTAAGTGGGGCTTCACGTCGTTCTGCTAGTACAGAATACTTGAGCAATGCTTTGAAAGTGGAGGAGGTGAATGATATAGACAGTATATTGCATGCATGTATAGGATTGTGCCAACGGAACACCCATCGTCTAAATCCTGATGAATCAGAGGCACTCTGGTTCAAATTGCTTGACTCGTTTTGTGAGCCTTTAATGGATTCGTTTAGTGCTGGTACAGTATCTAAAGGAGAAGATCATAATAGAATGGTAACCAATTCATTGGATTCACAAGAGGATGAAATGAATTTCACAATCAAGTGGAGAATCCCAAAGGCTCATAAAGGTGCTGATATTTTAAGAAAATTGTTTTCTCGGTTCATCAAGGAGATTGTTGAGGGCATGATAGGATATGTTCGTCTTCCAACTATCATGTCAAAGCTCCTTTCTGACAATGGTAGCCAGGAATTTGGTGATTTTAAATTCACAATATTGGGGATGCTTAGCACATATGGCTTTGAAAGAAGAATTCTGGATACTGCCAAGTCCCTGATAGAAGATGATACATTCTATACTATGAGCATCCTCAAGAAAGGAGCCTCACATGGGTATGCTCCTCGCAACCCAAAATGTTGCATATGCGATTGCCTCCTTGACAAGAACTCTTCCAGCTACATTCGCATTTTCAATTGTGGTCATGCAACACATCTTAAATGCGAAGTTTCAGAGAATGAAACATCAAGTCGTAGCTCGTCCTCTGGATGCCCAGTTTGTATGCCCAAGAAGAAATCTCAGAGGTCTAAAAACAAATCGGTTCTAGCAGAGGAGAGTTTAGTGAAGGTGTTTTCATCAAGAACCCATCATACTCATGGAACAGTTGTTCACCCACATGAAAGCACTTCTGAGAATAGCTATGGGATTCAGCAGATATCACGATTTGAGATGTTGACCAATTTGCAGAAACATCGTGGATTAGTCGAGATTGAAAACATGCCTCAATTGAGGCTTGCCCCACCAGCTGTGTACCATGAAAAGGTTAAGCACGGACCTGTTCTGTCACCAGGAGAGAGCAGCAGTGATCTTGTGACAACAGAGAAACAAAGTAAAATCAAGCAACTCAGGGAGATATCCACTTTCCAGGCAAGGACAAGATCAGCAGGCGATGATACTACTGCCATCTACAACTAA

Protein Analysis

1966

Amino Acids

217.36

Weight (kDa)

5.3

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_VPS8 PF23410 455 - 639 9.8e-23 Vps8 beta-propeller
VPS8_N PF23306 456 - 629 1.8e-06 VPS8-like, N-terminal beta propeller repeats
Beta-prop_WDR36-Utp21_1st PF25171 483 - 575 7e-06 WDR36/Utp21 first beta-propeller
TPR_Vps8 PF12816 1003 - 1186 5.9e-51 Vps8 central TPR
TPR_Vps41 PF23556 1498 - 1566 1.3e-06 Vps41 TPR-like region
TPR_VPS8_2 PF25066 1618 - 1765 2.1e-06 VPS8-like, TPR-like repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1506, 2529
AarI CACCTGC 1 cut(s) 826
AasI GACNNNNNNGTC 1 cut(s) 491
Acc16I TGCGCA 1 cut(s) 5
Acc36I ACCTGC 3 cut(s) 826, 1991, 3934
AccB1I GGYRCC 1 cut(s) 4355
AccB7I CCANNNNNTGG 1 cut(s) 3902
AccBSI CCGCTC 3 cut(s) 401, 645, 1558
AccII CGCG 8 cut(s) 310, 338, 563, 569, 614, 1375, 3186, 4578
AclWI GGATC 9 cut(s) 338, 1611, 2019, 2032, 2271, 2844, 3736, 3760, 4413
AcoI YGGCCR 4 cut(s) 60, 437, 2686, 3767
AcuI CTGAAG 2 cut(s) 2244, 3546
AcyI GRCGYC 3 cut(s) 366, 602, 746
AfiI CCNNNNNNNGG 9 cut(s) 645, 1374, 2611, 3139, 3371, 3902, 3961, 5319, 5746
AflIII ACRYGT 1 cut(s) 1742
AjiI CACGTC 1 cut(s) 4754
AjuI GAANNNNNNNTTGG 4 cut(s) 3889, 3921, 4707, 4739
AloI GAACNNNNNNTCC 2 cut(s) 5577, 5609
Alw21I GWGCWC 5 cut(s) 165, 2877, 3463, 4587, 4669
Alw26I GTCTC 8 cut(s) 42, 1297, 2328, 2994, 3844, 3968, 4447, 4553
Alw44I GTGCAC 2 cut(s) 2873, 4665
AlwI GGATC 9 cut(s) 338, 1611, 2019, 2032, 2271, 2844, 3736, 3760, 4413
AlwNI CAGNNNCTG 2 cut(s) 1763, 2371
Ama87I CYCGRG 1 cut(s) 554
ApaLI GTGCAC 2 cut(s) 2873, 4665
ArsI GACNNNNNNTTYG 2 cut(s) 2942, 2974
AseI ATTAAT 1 cut(s) 4479
Asp700I GAANNNNTTC 5 cut(s) 1918, 2012, 2619, 3239, 4110
AspLEI GCGC 3 cut(s) 6, 565, 638
AsuC2I CCSGG 7 cut(s) 441, 461, 472, 555, 556, 640, 2596
AsuII TTCGAA 1 cut(s) 758
AsuNHI GCTAGC 1 cut(s) 3734
AvaI CYCGRG 1 cut(s) 554
BaeGI GKGCMC 3 cut(s) 2877, 3208, 4669
BalI TGGCCA 1 cut(s) 2688
BamHI GGATCC 1 cut(s) 2024
BanI GGYRCC 1 cut(s) 4355
BanII GRGCYC 1 cut(s) 165
BbsI GAAGAC 2 cut(s) 3851, 5126
Bbv12I GWGCWC 5 cut(s) 165, 2877, 3463, 4587, 4669
BceAI ACGGC 7 cut(s) 228, 353, 496, 502, 733, 3660, 3754
BcgI CGANNNNNNTGC 8 cut(s) 577, 611, 3788, 3822, 4148, 4182, 5660, 5694
BciVI GTATCC 4 cut(s) 1334, 1613, 3305, 5242
BclI TGATCA 5 cut(s) 1054, 1099, 1405, 2362, 3787
BcnI CCSGG 7 cut(s) 441, 461, 472, 555, 556, 640, 2596
BcoDI GTCTC 8 cut(s) 42, 1297, 2328, 2994, 3844, 3968, 4447, 4553
BfmI CTRYAG 1 cut(s) 3623
BfoI RGCGCY 1 cut(s) 639
BfuAI ACCTGC 3 cut(s) 826, 1991, 3934
BfuI GTATCC 4 cut(s) 1334, 1613, 3305, 5242
BglII AGATCT 1 cut(s) 2967
BlpI GCTNAGC 1 cut(s) 5216
BmcAI AGTACT 2 cut(s) 2869, 3338
BmeT110I CYCGRG 1 cut(s) 554
BmgBI CACGTC 1 cut(s) 4754
BmrI ACTGGG 3 cut(s) 1482, 1773, 5474
BmtI GCTAGC 1 cut(s) 3738
BmuI ACTGGG 3 cut(s) 1482, 1773, 5474
BpiI GAAGAC 2 cut(s) 3851, 5126
BplI GAGNNNNNCTC 4 cut(s) 397, 429, 3477, 3509
BpmI CTGGAG 5 cut(s) 1193, 1262, 1683, 3930, 4532
Bpu1102I GCTNAGC 1 cut(s) 5216
Bpu14I TTCGAA 1 cut(s) 758
BpuEI CTTGAG 6 cut(s) 1328, 3451, 3980, 4354, 4798, 5285
BpuMI CCSGG 7 cut(s) 441, 461, 472, 555, 556, 640, 2596
BsaAI YACGTR 1 cut(s) 1745
BsaBI GATNNNNATC 3 cut(s) 3162, 3786, 4572
BsaHI GRCGYC 3 cut(s) 366, 602, 746
BsaI GGTCTC 2 cut(s) 2328, 4447
BsaWI WCCGGW 2 cut(s) 481, 1682
Bsc4I CCNNNNNNNGG 9 cut(s) 645, 1374, 2611, 3139, 3371, 3902, 3961, 5319, 5746
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 8 cut(s) 1176, 1488, 1700, 1768, 2395, 2651, 4525, 5480
Bse3DI GCAATG 6 cut(s) 730, 874, 1446, 2676, 3131, 4789
Bse8I GATNNNNATC 3 cut(s) 3162, 3786, 4572
BseJI GATNNNNATC 3 cut(s) 3162, 3786, 4572
BseLI CCNNNNNNNGG 9 cut(s) 645, 1374, 2611, 3139, 3371, 3902, 3961, 5319, 5746
BseMI GCAATG 6 cut(s) 730, 874, 1446, 2676, 3131, 4789
BseNI ACTGG 8 cut(s) 1176, 1488, 1700, 1768, 2395, 2651, 4525, 5480
BseSI GKGCMC 3 cut(s) 2877, 3208, 4669
BseX3I CGGCCG 1 cut(s) 437
BsgI GTGCAG 1 cut(s) 2355
Bsh1236I CGCG 8 cut(s) 310, 338, 563, 569, 614, 1375, 3186, 4578
Bsh1285I CGRYCG 4 cut(s) 252, 378, 440, 495
BshNI GGYRCC 1 cut(s) 4355
BsiEI CGRYCG 4 cut(s) 252, 378, 440, 495
BsiHKAI GWGCWC 5 cut(s) 165, 2877, 3463, 4587, 4669
BsiHKCI CYCGRG 1 cut(s) 554
BsiWI CGTACG 1 cut(s) 245
BslFI GGGAC 4 cut(s) 221, 1469, 3596, 5245
BslI CCNNNNNNNGG 9 cut(s) 645, 1374, 2611, 3139, 3371, 3902, 3961, 5319, 5746
BsmAI GTCTC 8 cut(s) 42, 1297, 2328, 2994, 3844, 3968, 4447, 4553
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 4 cut(s) 221, 1469, 3596, 5245
BsmI GAATGC 4 cut(s) 550, 2810, 4232, 4538
Bso31I GGTCTC 2 cut(s) 2328, 4447
BsoBI CYCGRG 1 cut(s) 554
Bsp119I TTCGAA 1 cut(s) 758
Bsp1286I GDGCHC 6 cut(s) 165, 2877, 3208, 3463, 4587, 4669
Bsp1407I TGTACA 3 cut(s) 453, 1705, 2731
Bsp1720I GCTNAGC 1 cut(s) 5216
Bsp19I CCATGG 1 cut(s) 156
BspFNI CGCG 8 cut(s) 310, 338, 563, 569, 614, 1375, 3186, 4578
BspHI TCATGA 1 cut(s) 1057
BspMAI CTGCAG 1 cut(s) 3627
BspMI ACCTGC 3 cut(s) 826, 1991, 3934
BspOI GCTAGC 1 cut(s) 3738
BspPI GGATC 9 cut(s) 338, 1611, 2019, 2032, 2271, 2844, 3736, 3760, 4413
BspQI GCTCTTC 2 cut(s) 1230, 1924
BspT104I TTCGAA 1 cut(s) 758
BspT107I GGYRCC 1 cut(s) 4355
BspTNI GGTCTC 2 cut(s) 2328, 4447
BsrBI CCGCTC 3 cut(s) 401, 645, 1558
BsrDI GCAATG 6 cut(s) 730, 874, 1446, 2676, 3131, 4789
BsrFI RCCGGY 1 cut(s) 58
BsrGI TGTACA 3 cut(s) 453, 1705, 2731
BsrI ACTGG 8 cut(s) 1176, 1488, 1700, 1768, 2395, 2651, 4525, 5480
BssAI RCCGGY 1 cut(s) 58
BssNI GRCGYC 3 cut(s) 366, 602, 746
BssT1I CCWWGG 2 cut(s) 156, 3307
Bst6I CTCTTC 9 cut(s) 307, 1230, 1272, 1924, 2018, 3060, 3595, 3928, 5383
BstACI GRCGYC 3 cut(s) 366, 602, 746
BstAPI GCANNNNNTGC 1 cut(s) 1667
BstAUI TGTACA 3 cut(s) 453, 1705, 2731
BstBAI YACGTR 1 cut(s) 1745
BstBI TTCGAA 1 cut(s) 758
BstDSI CCRYGG 4 cut(s) 156, 518, 612, 2606
BstEII GGTNACC 2 cut(s) 3246, 4984
BstENI CCTNNNNNAGG 1 cut(s) 3959
BstFNI CGCG 8 cut(s) 310, 338, 563, 569, 614, 1375, 3186, 4578
BstH2I RGCGCY 1 cut(s) 639
BstHHI GCGC 3 cut(s) 6, 565, 638
BstMAI GTCTC 8 cut(s) 42, 1297, 2328, 2994, 3844, 3968, 4447, 4553
BstMCI CGRYCG 4 cut(s) 252, 378, 440, 495
BstNSI RCATGY 6 cut(s) 2108, 3046, 3825, 4832, 4836, 5707
BstPI GGTNACC 2 cut(s) 3246, 4984
BstSFI CTRYAG 1 cut(s) 3623
BstSLI GKGCMC 3 cut(s) 2877, 3208, 4669
BstUI CGCG 8 cut(s) 310, 338, 563, 569, 614, 1375, 3186, 4578
BstV2I GAAGAC 2 cut(s) 3851, 5126
BstX2I RGATCY 4 cut(s) 1603, 2024, 2967, 3728
BstXI CCANNNNNNTGG 7 cut(s) 1336, 2347, 2696, 3725, 4037, 4996, 5183
BstYI RGATCY 4 cut(s) 1603, 2024, 2967, 3728
BstZI CGGCCG 1 cut(s) 437
BsuI GTATCC 4 cut(s) 1334, 1613, 3305, 5242
BtgI CCRYGG 4 cut(s) 156, 518, 612, 2606
BtgZI GCGATG 2 cut(s) 1958, 5889
BtrI CACGTC 1 cut(s) 4754
BtsI GCAGTG 2 cut(s) 3745, 5794
BtsIMutI CAGTG 7 cut(s) 912, 1847, 2877, 3745, 4338, 4585, 5794
BveI ACCTGC 3 cut(s) 826, 1991, 3934
CaiI CAGNNNCTG 2 cut(s) 1763, 2371
CciI TCATGA 1 cut(s) 1057
CfoI GCGC 3 cut(s) 6, 565, 638
Cfr10I RCCGGY 1 cut(s) 58
Cfr42I CCGCGG 1 cut(s) 615
Cfr9I CCCGGG 1 cut(s) 554
CpoI CGGWCCG 1 cut(s) 433
CseI GACGC 5 cut(s) 327, 374, 610, 754, 1882
CsiI ACCWGGT 2 cut(s) 2754, 4029
CspCI CAANNNNNGTGG 2 cut(s) 4443, 4478
CspI CGGWCCG 1 cut(s) 433
DraI TTTAAA 2 cut(s) 4377, 5194
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
EaeI YGGCCR 4 cut(s) 60, 437, 2686, 3767
EagI CGGCCG 1 cut(s) 437
Eam1104I CTCTTC 9 cut(s) 307, 1230, 1272, 1924, 2018, 3060, 3595, 3928, 5383
EarI CTCTTC 9 cut(s) 307, 1230, 1272, 1924, 2018, 3060, 3595, 3928, 5383
EciI GGCGGA 1 cut(s) 402
Ecl136II GAGCTC 1 cut(s) 163
EclXI CGGCCG 1 cut(s) 437
Eco130I CCWWGG 2 cut(s) 156, 3307
Eco147I AGGCCT 1 cut(s) 3351
Eco24I GRGCYC 1 cut(s) 165
Eco31I GGTCTC 2 cut(s) 2328, 4447
Eco32I GATATC 2 cut(s) 5643, 5841
Eco52I CGGCCG 1 cut(s) 437
Eco53kI GAGCTC 1 cut(s) 163
Eco57I CTGAAG 2 cut(s) 2244, 3546
Eco88I CYCGRG 1 cut(s) 554
Eco91I GGTNACC 2 cut(s) 3246, 4984
EcoICRI GAGCTC 1 cut(s) 163
EcoNI CCTNNNNNAGG 1 cut(s) 3959
EcoO65I GGTNACC 2 cut(s) 3246, 4984
EcoRI GAATTC 7 cut(s) 754, 892, 2924, 3473, 3985, 4197, 5241
EcoRV GATATC 2 cut(s) 5643, 5841
EcoT14I CCWWGG 2 cut(s) 156, 3307
EcoT22I ATGCAT 2 cut(s) 1126, 4834
EcoT38I GRGCYC 1 cut(s) 165
ErhI CCWWGG 2 cut(s) 156, 3307
Esp3I CGTCTC 1 cut(s) 42
FalI AAGNNNNNCTT 6 cut(s) 2539, 2571, 3326, 3358, 4732, 4764
FaqI GGGAC 4 cut(s) 221, 1469, 3596, 5245
FauI CCCGC 3 cut(s) 315, 394, 638
FauNDI CATATG 3 cut(s) 2776, 5224, 5351
FbaI TGATCA 5 cut(s) 1054, 1099, 1405, 2362, 3787
FriOI GRGCYC 1 cut(s) 165
FspAI RTGCGCAY 1 cut(s) 5
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 3 cut(s) 5, 564, 637
GsuI CTGGAG 5 cut(s) 1193, 1262, 1683, 3930, 4532
HaeII RGCGCY 1 cut(s) 639
HgaI GACGC 5 cut(s) 327, 374, 610, 754, 1882
HhaI GCGC 3 cut(s) 6, 565, 638
Hin1I GRCGYC 3 cut(s) 366, 602, 746
Hin6I GCGC 3 cut(s) 4, 563, 636
HinP1I GCGC 3 cut(s) 4, 563, 636
HincII GTYRAC 5 cut(s) 411, 2707, 3745, 4152, 5661
HindII GTYRAC 5 cut(s) 411, 2707, 3745, 4152, 5661
HindIII AAGCTT 4 cut(s) 128, 1254, 2523, 4709
HinfI GANTC 4 cut(s) 19, 173, 212, 275
Hpy99I CGWCG 9 cut(s) 257, 368, 517, 604, 748, 754, 1631, 1876, 4758
HpyCH4IV ACGT 3 cut(s) 1744, 3899, 4753
HpySE526I ACGT 3 cut(s) 1744, 3899, 4753
Hsp92I GRCGYC 3 cut(s) 366, 602, 746
HspAI GCGC 3 cut(s) 4, 563, 636
Ksp22I TGATCA 5 cut(s) 1054, 1099, 1405, 2362, 3787
KspI CCGCGG 1 cut(s) 615
LguI GCTCTTC 2 cut(s) 1230, 1924
MabI ACCWGGT 2 cut(s) 2754, 4029
MaeII ACGT 3 cut(s) 1744, 3899, 4753
MbiI CCGCTC 3 cut(s) 401, 645, 1558
MfeI CAATTG 7 cut(s) 416, 2183, 2253, 2533, 3678, 5401, 5711
MflI RGATCY 4 cut(s) 1603, 2024, 2967, 3728
MhlI GDGCHC 6 cut(s) 165, 2877, 3208, 3463, 4587, 4669
MlsI TGGCCA 1 cut(s) 2688
MluNI TGGCCA 1 cut(s) 2688
MlyI GAGTC 6 cut(s) 167, 206, 406, 850, 3740, 4904
Mox20I TGGCCA 1 cut(s) 2688
Mph1103I ATGCAT 2 cut(s) 1126, 4834
MroXI GAANNNNTTC 5 cut(s) 1918, 2012, 2619, 3239, 4110
MscI TGGCCA 1 cut(s) 2688
Msp20I TGGCCA 1 cut(s) 2688
MspA1I CMGCKG 6 cut(s) 384, 614, 1667, 3605, 4103, 5732
MunI CAATTG 7 cut(s) 416, 2183, 2253, 2533, 3678, 5401, 5711
Mva1269I GAATGC 4 cut(s) 550, 2810, 4232, 4538
MvnI CGCG 8 cut(s) 310, 338, 563, 569, 614, 1375, 3186, 4578
NciI CCSGG 7 cut(s) 441, 461, 472, 555, 556, 640, 2596
NcoI CCATGG 1 cut(s) 156
NdeI CATATG 3 cut(s) 2776, 5224, 5351
NheI GCTAGC 1 cut(s) 3734
NmuCI GTSAC 8 cut(s) 1631, 1738, 2059, 2642, 3246, 4483, 5769, 5797
NsbI TGCGCA 1 cut(s) 5
NsiI ATGCAT 2 cut(s) 1126, 4834
NspI RCATGY 6 cut(s) 2108, 3046, 3825, 4832, 4836, 5707
NspV TTCGAA 1 cut(s) 758
PaeI GCATGC 1 cut(s) 4832
PagI TCATGA 1 cut(s) 1057
PaqCI CACCTGC 1 cut(s) 826
PceI AGGCCT 1 cut(s) 3351
PciSI GCTCTTC 2 cut(s) 1230, 1924
PcsI WCGNNNNNNNCGW 1 cut(s) 749
PctI GAATGC 4 cut(s) 550, 2810, 4232, 4538
PdmI GAANNNNTTC 5 cut(s) 1918, 2012, 2619, 3239, 4110
Pfl23II CGTACG 1 cut(s) 245
PflFI GACNNNGTC 2 cut(s) 748, 1876
PflMI CCANNNNNTGG 1 cut(s) 3902
Ple19I CGATCG 1 cut(s) 252
PleI GAGTC 6 cut(s) 167, 206, 406, 849, 3740, 4904
PpsI GAGTC 6 cut(s) 167, 206, 406, 849, 3740, 4904
Ppu21I YACGTR 1 cut(s) 1745
PshBI ATTAAT 1 cut(s) 4479
PsiI TTATAA 2 cut(s) 1506, 2529
Psp124BI GAGCTC 1 cut(s) 165
PspEI GGTNACC 2 cut(s) 3246, 4984
PspLI CGTACG 1 cut(s) 245
PsrI GAACNNNNNNTAC 2 cut(s) 5560, 5592
PstI CTGCAG 1 cut(s) 3627
PstNI CAGNNNCTG 2 cut(s) 1763, 2371
PsuI RGATCY 4 cut(s) 1603, 2024, 2967, 3728
PsyI GACNNNGTC 2 cut(s) 748, 1876
PvuI CGATCG 1 cut(s) 252
PvuII CAGCTG 3 cut(s) 3605, 4103, 5732
Rsr2I CGGWCCG 1 cut(s) 433
RsrII CGGWCCG 1 cut(s) 433
SacI GAGCTC 1 cut(s) 165
SacII CCGCGG 1 cut(s) 615
SapI GCTCTTC 2 cut(s) 1230, 1924
ScaI AGTACT 2 cut(s) 2869, 3338
SchI GAGTC 6 cut(s) 167, 206, 406, 850, 3740, 4904
SduI GDGCHC 6 cut(s) 165, 2877, 3208, 3463, 4587, 4669
SexAI ACCWGGT 2 cut(s) 2754, 4029
SfcI CTRYAG 1 cut(s) 3623
Sfr303I CCGCGG 1 cut(s) 615
SfuI TTCGAA 1 cut(s) 758
SgrBI CCGCGG 1 cut(s) 615
SmaI CCCGGG 1 cut(s) 556
SmlI CTYRAG 6 cut(s) 1307, 3466, 3959, 4369, 4777, 5300
SmoI CTYRAG 6 cut(s) 1307, 3466, 3959, 4369, 4777, 5300
SphI GCATGC 1 cut(s) 4832
SseBI AGGCCT 1 cut(s) 3351
SspI AATATT 1 cut(s) 5205
SstI GAGCTC 1 cut(s) 165
StuI AGGCCT 1 cut(s) 3351
StyI CCWWGG 2 cut(s) 156, 3307
TaiI ACGT 3 cut(s) 1747, 3902, 4756
TaqII GACCGA 2 cut(s) 364, 481
TscAI CASTG 7 cut(s) 919, 1847, 2884, 3745, 4338, 4592, 5794
TseFI GTSAC 8 cut(s) 1631, 1738, 2059, 2642, 3246, 4483, 5769, 5797
Tsp45I GTSAC 8 cut(s) 1631, 1738, 2059, 2642, 3246, 4483, 5769, 5797
TspGWI ACGGA 6 cut(s) 60, 223, 2632, 2647, 4867, 5772
TspMI CCCGGG 1 cut(s) 554
TspRI CASTG 7 cut(s) 919, 1847, 2884, 3745, 4338, 4592, 5794
Tth111I GACNNNGTC 2 cut(s) 748, 1876
Van91I CCANNNNNTGG 1 cut(s) 3902
VneI GTGCAC 2 cut(s) 2873, 4665
VspI ATTAAT 1 cut(s) 4479
XagI CCTNNNNNAGG 1 cut(s) 3959
XbaI TCTAGA 3 cut(s) 1849, 2449, 4193
XceI RCATGY 6 cut(s) 2108, 3046, 3825, 4832, 4836, 5707
XcmI CCANNNNNNNNNTGG 1 cut(s) 5579
XmaI CCCGGG 1 cut(s) 554
XmnI GAANNNNTTC 5 cut(s) 1918, 2012, 2619, 3239, 4110
ZrmI AGTACT 2 cut(s) 2869, 3338
Zsp2I ATGCAT 2 cut(s) 1126, 4834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.