RLG00000027750

Vacuolar protein sorting-associated protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006617
Physical Location & Seq
Reverse (-)
14798504 .. 14807844
9341 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027750

Sequence Viewer

Length: 5817 bp
ATGACCAAGAAGCTCACACAATCGGAACCACAACTCGCCATGGAGCTCGACCTCGACTCGTTCCTCAACTCACACCTCTCCTCCTCCGACTCCGACCCCGACCTCACCTCCGTCCCTCGCCGTACGATCGACGAAATCCTCAACGACACCGATTCATCCAGGTCAGCATCTCCCTCCTCTTCCCCGCGTAGTTCGACCTTATCTCAGTTCCTCGCGTCCGATCCCAAACCGCCACAGAGCGACGCCGTTTCGGTCGCCAGTGGCAGAACCGAGGGAGCGGGGCGGTTGAGTCAATTGGAGGAGAAGGCGGTCCGGCCGGGTTTGAATTTGTACAGCCGGGCTAGGTCCGGGGATTTCCCGGACGATTCGGTCGGGAGAGTTTCGAGGCCGTCGCCGTGGCTGTTGGGAGGTGTGAGGACGAATGCGAAGCCCGGGGCGGCGCTCGCGGCGGCTGCGGCGGCTTCGAGGTCGATGCCGACGCCGCACGCCGCGGCGATCAAGTCGAGGAGGAGCGCCGGGAGCGGGAGTTTCCAGAAGGCTGTGGAGGCTGCGGAATTGGGTGAGAGATCGGAGGGAGGGTCCAATTTTAACGAGGATAGCAATGAGGTTGGTTCCGAAACGACGCCGTCGCATTCGAATGAAGTAGAGGAGGATTTTGGTGGTGAGTTGGGGATGAAAGATGAGGTCTTGGAGAGGGAGAGGGAGGTAGAGCAGGTGAGTCAGGTTGATGAAGTTAGTGCTGGCAATGCTGGTGAAGAATTGGATGAGAATTCAACAAATTTAGATGCCACTGATGTTAGAGATAATGAATTGGATGAGAATGTTCGGGTTTCGGAGGAAGGTCCACCAGAGGAAGAAGATTTAGATGAAAATAGTCTTTCTTCGAAACATAGTGATGTTAAGGATAAGCCTACTGGTGAGGATGAGCAGCATCTTGGTGGTATTGATGATAATGATGAGGTTGGTGATAATGATAATGATAAAGATGGTGATGATCATTTTGATGATGATGATGCATTGGGAACTTCAATCACTCAACTTGTGGAGGAGAGAATCGAGCAACTGGAGAGCAGGAGGATTAGTAAAAAAGCGGAAAAGAAATCAAGAAAGCCACTTGAAATCGCCGAGGAGCTAGAGAAGAAACAAGCTTCAACTGCTTTGGATTGGGAAGAGGGTGCTGCTGCTCAACCTATGAGACTTGAGGGTGTTCGGAGAGGGTCCACCACATTGGGATACTTCAATGTTGATGCTAAAAATACCATTACGCGTACCCTTTCTGCTCCGGCTTTGAGGCGTGATCATGGTACGCCTCAAGTCCTTGGAGTTCACAGTAATTACATTGCAATAGGAATGTCAAGAGGGGTTGTCCTTGTTGTCCCCAGTAAATACTCTCCTTATAATGCTGACAACATGGATGCAAAGCTGATATTTCTTGGATTGCAAGGAGAGCGGTCTTATGCTGCGGTAACTTCTATATCCTTCAATCAGCAAGGGGATCTACTCTTAGCTGGATACGCCGATGGTCACATTACAGTTTGGGATGTTCAGAGGGCATCTGCTGCCAAAGTAATTACCGGAGAACATACAGCTCCAGTTGTACATACTTTTTTTTTGGGGCATGATTCTCAGGTCACACGTAATTTTAAGGCAGTTACTGGGGATAGTAAGGGTCTGGTACTGTTACATTCCTTCTCTGTGGTTCCCCTGCTTAATAGATTCTCCATCAAAACACAGTGCCTTCTAGATGGACAAAAAACAGGCACGACGCTGTCAGCTTCACCACTCCTCATTGATGAATTGAGTGGAGGCTCTTCACTTTCCTCTCAGGGAAATGCAATGGGTTCAGGCAGCAGTATTGGTGGTATGATGGGTGGTGTGGTTGGTGGGGATGCAGGTTGGAAACTCTTCAATGAAGGATCCTCTTTGTTTGAAGAAGGTGTGGTCGTATTTGTCACCCATCAAAATGCTCTGGTGGTAAGACTAACTCCCACTTTGCATGTCTATGCACAACTCTCTAAACCAGATGGGGTTCGGGAGGGTTCCATGCCTTGTACTGCTTGGAAATGCACAACTCAATTGCATAATTCACCTACTAATAGTGAGAATACGCCTACTGAAGCAGTTGAAAGAGTTTCATTACTTGCAATTGCTTGGGATCGGAAAGTTCAGGTAGCAAAGTTGGTCAAATCAGAGCTGAAAGTATATGGTAAATGGTCTCTTGAAAGTGCAGCCATAGGTGTGGCTTGGTTGGATGATCAGATGCTGGTGGTTCTCACGATGACTGGACAACTTTGTTTGTTTGCAAAGGATGGAACTGCAATTCACCAAACAAGTTTTTCTCTAGATGGTTTTAGAGGGGATGATCTCATTTCTTATCATACTCACTTCATCAATGTATTTGGAAACCCTGAAAAAGCTTATAACAATTGCATAGCTGTAAGAGGAGCTTCTGTCTATGTACTTGGACCTACTCATCTTATTGTTTCCCGGCTTCTCCCATGGAAGGAACGGATTCAGGTTTTACGGAGAGCTGGTGACTGGATGGGTTCTTTGAACATGGCAATGACAATTTATGATGGCCAAGCTCATGGTGTTGTTGACCTTCCTAGAACTCTGGATGCTGTACAGGAGGTCATAATGCCCTACCTGGTTGAGTTGCTTTTGTCATATGTAGAGGAAGTTTTTTCTTATATTTCAGTAGCATTCTGCAACCAAATTGAAAAAATGGATCAAGTTGATGACTTAAGCAGACAAAATAGTACTGTGCACACTGAAATTAAAGAGCAATACACCCGTGTTGGTGGGGTTGCAGTTGAATTCTGTGTCCATATCAAGAGGACTGACATACTTTTTGACGAGATCTTCTTGAAGTTTGTAGCTGTTCAACAGAGAGACACATTTTTGGAACTATTGGAGCCTTATATATTGAAGGACATGCTTGGTTCTCTCCCGCCTGAGATTATGCAAGCTCTGGTAGAACATTACAGCCACAAAGGGTGGTTACAGCGTGTTGAGCAATGTGTCCTTCACATGGATATCTCTTCATTGGATTTCAATCAGGTTGTTAGGTTATGCCGCGAGCATGGATTATATAGTGCTCTGGTGTATCTTTTCAATAAAGGATTGAATGATTTCAGGTCACCATTAGAGGAGCTGTTGGTGGTCTTGCGAAATAGTCAAAAAGAAGGTGCTATGGCCCTTGGATACAGGATGCTAGTTTATTTGAAGTACTGCTTTTCAGGCCTTGCTTTTCCACCAGGACAAGGAAAAATTCCTCCTTCACGCTTGCCATCTCTCAGAACAGAACTTTTGCAGTTTTTATTGGAGGGTTCTGATGCCCCTAATTCAAGAGCACTCTCAAGTGAATTCCCAGAAGGAGAATATCTCAACCTTTATTATCTCTTAGAGCTAGATACTGAAGCTACTTTAGATGTTCTGAGATGTGCTTTCACAGAAGTTGAAATCTCGAAATGGGACTCCTCTTCTCAAAATTCAGCTGATGCAGATACAGAGCTGCAGTATGGGAATAATTCAATGGCTCAAAGTCAGGATTCATTGGTCCAGAATACAATTGATACTCTTATTCACATTATTAGTAAGGACATTCCCCAGAAGGATGGATCTGCTAGCAGTGTTGACTCTGGATCAGTTGTGGAGTGGCCTTCAAAGAAAGATATTGATCATCTATTCGAGTTTATTGCATGTTATGTTGCATGTGGAAGAGCTAATGTCTCGAAGACTGTACTTTCTCAGATTTTGGAGTATTTGACATCAGATAATAATTTTCCAACGTCTGTTTCTGGAGATAGCATGATTTCAAAAAGAAGAGAGAAGCAGGTTCTTGGCCTTCTTGAGGTAGTGCCGGAGACTGATTGGAATTCATCTTCTGTGTTACAGCTATGTGAGAAAGCACAATTTTACCAGGTATGTGGCCTAATTCATACCAGCAGACATCAGTATATTGCTGCCCTGGATTGCTATATGAAAGATGCAGCCGAACCCATTCATGCCTTTGCCTTTATCAACAAAACATTGTTACAGTTGACTGATGATGAATGTGCTGCTTTTCGATCAGCAGTTATTTCTAGAATTGCAGAGCTATTTAACTTAAACAGAGAGGGTGCATTCTTCTTGGTCATGGACCATTTTACCGGTGAAGAAGGTTCTCATATCCTATCTGAACTTCGCTCCCATCCACAAAGCCTTTTCCTGTACTTAAAAACAGTGATTGAGGTTCATTTATCCGGCACCCTCGACTTTTCAAGTTTAAAAAACGACAATCTTATGGGAGTAAAGGATCAAACAAAAGAAGTTGAGGCTTTCTTGGAAAGGGTCTCTAATTTCCCACAGCTTTTGCGTGATAGTCCTATTAATGTGACTGATGATATGATTGAGCTTTATCTGGAGCTGTTATGCCAGTTTGAACGCAAATCAGTTCTCAAATTCCTAGAGACATTTGATAGCTATCGCGTGGAGCACTGTTTACGCCTCTGCCAGAAATATGAAATAATAGATGCTTCATCATTCTTGTTGGAGAGGGTTGGTGATGTTGGAAGTGCTCTTTTACTTACACTATCCACCCTTAATGACAAATTTATCAAGCTTGAAACTTCTGTTGGAAGTCTAAATTTAAGTGGGGTCTCATGTGGTTCTGCTAGTACAGAATACTTGAGCAATGCTTTGAAAGTGGAGGAGGTGAATGATATAGACAGTATATTGCATGCATGTATAGGATTGTGCCAACGGAACACCCATCGTCTAAATCCTGATGAATCAGAGGCACTCTGGTTCAAATTGCTTGACTCGTTTTGTGAGCCTTTAATGGATTCGTTCAGTTTTGGTACAGTATCTAAAGGAGAAGATCATAATAGAATGGTAACCAATTCATTGGATTCACACGAGGATGAAATGAATTTCACAATCAAGTGGAGAATCCCAAAGGCCCATAAAGGTGCTGATATTTTAAGAAAATTGTTTTCTCGGTTCATCAAGGAGATTGTTGAGGGCATGATAGGATATGTTCGCCTTCCAACTATCATGTCAAAGCTCCTTTCTGACAATGGTAGCCAGGAATTTGGTGATTTCAAATTCACAATATTGGGGATGCTTAGCACATATGGCTTCGAAAGAAGAATTCTGGATACTGCCAAGTCCTTGATAGAAGATGATACATTCTATACTATGAGCATCCTCAAGAAAGGAGCCTCACATGGGTATGCTCCTCGCAACCCAAAATGTTGCATATGCGATTGCCTCCTTGACAAGAACTCTTCCAGCTACATTCGCATTTTCAATTGTGGTCATGCAACACATCTTAAATGCGAAGTTTCAGAGAATGAAACATCAAGTCGTAGCTCGTCCTCTGGATGCCCTGTTTGTATGCCCAAGAAGAAATCTCAGAGGTCTAAAAACAAATCGGTTCTAGCAGAGGAGAGTTTAGTGAAGGTGTTTTCATCAAGAACCCATCATACTCATGGAACAGTTGTTCACCCACATGAAAGCAGTGCTTCTGAGAATAGCTATGGGATTCAGCAGATATCACGATTTGAGATGTTGACCAATTTGCAGAAACATCGTGGATTAGTTGAGATTGAAAACATGCCTCAATTGAGGCTTGCCCCACCAGCTGTGTACCATGAAAAGGTTAAGCACGGACCTGTTCTGTCACCAGGAGAGAGCAGCAGTGATCTTGTGAGAACAGAGAAACAAAGTAAAATCAAGCAACTCAGGGAGGTAAAAGTAAAAGGATCATCTCTTCGGTTTCCCTTGAAAACAAACATATTTGGTAATGGCAAGGACAAGATCAGCAGGCGATGA

Protein Analysis

1939

Amino Acids

214.41

Weight (kDa)

5.25

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_VPS8 PF23410 419 - 601 1.4e-22 Vps8 beta-propeller
VPS8_N PF23306 420 - 593 1.8e-06 VPS8-like, N-terminal beta propeller repeats
Beta-prop_WDR36-Utp21_1st PF25171 447 - 539 6.9e-06 WDR36/Utp21 first beta-propeller
TPR_Vps8 PF12816 967 - 1150 9.1e-52 Vps8 central TPR
TPR_Vps41 PF23556 1461 - 1530 8.7e-08 Vps41 TPR-like region
TPR_VPS8_2 PF25066 1582 - 1729 8.1e-06 VPS8-like, TPR-like repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1398, 2421
AarI CACCTGC 1 cut(s) 703
AasI GACNNNNNNGTC 1 cut(s) 368
Acc36I ACCTGC 3 cut(s) 703, 1883, 3826
AccB1I GGYRCC 1 cut(s) 4247
AccBSI CCGCTC 3 cut(s) 278, 522, 1450
AccII CGCG 7 cut(s) 187, 215, 446, 491, 1267, 3078, 4470
AcoI YGGCCR 2 cut(s) 314, 2578
AcuI CTGAAG 2 cut(s) 2136, 3438
AcyI GRCGYC 3 cut(s) 243, 479, 623
AfiI CCNNNNNNNGG 7 cut(s) 522, 2503, 3031, 3263, 3853, 5211, 5641
AflII CTTAAG 1 cut(s) 2743
AflIII ACRYGT 2 cut(s) 1265, 1634
AgeI ACCGGT 1 cut(s) 4151
AjnI CCWGG 7 cut(s) 158, 2646, 3256, 3921, 3969, 5067, 5668
AjuI GAANNNNNNNTTGG 4 cut(s) 3781, 3813, 4599, 4631
AloI GAACNNNNNNTCC 2 cut(s) 5469, 5501
Alw21I GWGCWC 6 cut(s) 48, 2769, 3100, 3355, 4479, 4561
Alw26I GTCTC 8 cut(s) 1189, 2220, 2886, 3736, 3860, 4339, 4445, 4645
Alw44I GTGCAC 1 cut(s) 2765
AlwNI CAGNNNCTG 2 cut(s) 1655, 2263
Ama87I CYCGRG 1 cut(s) 431
AoxI GGCC 9 cut(s) 314, 386, 2578, 3195, 3241, 3659, 3844, 3931, 4941
ApaLI GTGCAC 1 cut(s) 2765
ArsI GACNNNNNNTTYG 2 cut(s) 2834, 2866
AseI ATTAAT 1 cut(s) 4371
AsiGI ACCGGT 1 cut(s) 4151
Asp700I GAANNNNTTC 4 cut(s) 1904, 2511, 3131, 4002
AspLEI GCGC 2 cut(s) 442, 515
AsuC2I CCSGG 8 cut(s) 318, 338, 349, 359, 432, 433, 517, 2488
AsuII TTCGAA 3 cut(s) 635, 884, 5124
AsuNHI GCTAGC 1 cut(s) 3626
AvaI CYCGRG 1 cut(s) 431
AvaII GGWCC 9 cut(s) 310, 345, 579, 842, 1218, 2465, 3559, 4141, 5654
BaeGI GKGCMC 1 cut(s) 2769
BalI TGGCCA 1 cut(s) 2580
BamHI GGATCC 1 cut(s) 1916
BanI GGYRCC 1 cut(s) 4247
BanII GRGCYC 1 cut(s) 48
BauI CACGAG 1 cut(s) 4898
BbsI GAAGAC 1 cut(s) 3743
Bbv12I GWGCWC 6 cut(s) 48, 2769, 3100, 3355, 4479, 4561
BceAI ACGGC 5 cut(s) 105, 230, 373, 379, 610
BcgI CGANNNNNNTGC 6 cut(s) 454, 488, 3680, 3714, 5555, 5589
BciT130I CCWGG 7 cut(s) 160, 2648, 3258, 3923, 3971, 5069, 5670
BciVI GTATCC 4 cut(s) 1226, 1505, 3197, 5134
BclI TGATCA 4 cut(s) 994, 1297, 2254, 3679
BcnI CCSGG 8 cut(s) 318, 338, 349, 359, 432, 433, 517, 2488
BcoDI GTCTC 8 cut(s) 1189, 2220, 2886, 3736, 3860, 4339, 4445, 4645
BfmI CTRYAG 1 cut(s) 3515
BfoI RGCGCY 2 cut(s) 443, 516
BfrI CTTAAG 1 cut(s) 2743
BfuAI ACCTGC 3 cut(s) 703, 1883, 3826
BfuI GTATCC 4 cut(s) 1226, 1505, 3197, 5134
BglII AGATCT 1 cut(s) 2859
BlpI GCTNAGC 1 cut(s) 5108
BmcAI AGTACT 2 cut(s) 2761, 3230
Bme18I GGWCC 9 cut(s) 310, 345, 579, 842, 1218, 2465, 3559, 4141, 5654
BmeT110I CYCGRG 1 cut(s) 431
BmrI ACTGGG 2 cut(s) 1374, 1665
BmtI GCTAGC 1 cut(s) 3630
BmuI ACTGGG 2 cut(s) 1374, 1665
BpiI GAAGAC 1 cut(s) 3743
BplI GAGNNNNNCTC 2 cut(s) 3369, 3401
BpmI CTGGAG 4 cut(s) 1085, 1575, 3822, 4424
Bpu1102I GCTNAGC 1 cut(s) 5108
Bpu14I TTCGAA 3 cut(s) 635, 884, 5124
BpuEI CTTGAG 6 cut(s) 1220, 1296, 3343, 3872, 4690, 5177
BpuMI CCSGG 8 cut(s) 318, 338, 349, 359, 432, 433, 517, 2488
BsaAI YACGTR 1 cut(s) 1637
BsaBI GATNNNNATC 3 cut(s) 3054, 3678, 4464
BsaHI GRCGYC 3 cut(s) 243, 479, 623
BsaI GGTCTC 3 cut(s) 2220, 4339, 4645
BsaWI WCCGGW 2 cut(s) 1574, 4151
Bsc4I CCNNNNNNNGG 7 cut(s) 522, 2503, 3031, 3263, 3853, 5211, 5641
Bse118I RCCGGY 1 cut(s) 4151
Bse1I ACTGG 9 cut(s) 258, 919, 1068, 1380, 1592, 1660, 2287, 2543, 4417
Bse3DI GCAATG 7 cut(s) 607, 751, 1338, 1842, 2568, 3023, 4681
Bse8I GATNNNNATC 3 cut(s) 3054, 3678, 4464
BseBI CCWGG 7 cut(s) 160, 2648, 3258, 3923, 3971, 5069, 5670
BseJI GATNNNNATC 3 cut(s) 3054, 3678, 4464
BseLI CCNNNNNNNGG 7 cut(s) 522, 2503, 3031, 3263, 3853, 5211, 5641
BseMI GCAATG 7 cut(s) 607, 751, 1338, 1842, 2568, 3023, 4681
BseNI ACTGG 9 cut(s) 258, 919, 1068, 1380, 1592, 1660, 2287, 2543, 4417
BseSI GKGCMC 1 cut(s) 2769
BseX3I CGGCCG 1 cut(s) 314
BsgI GTGCAG 1 cut(s) 2247
Bsh1236I CGCG 7 cut(s) 187, 215, 446, 491, 1267, 3078, 4470
Bsh1285I CGRYCG 4 cut(s) 129, 255, 317, 372
BshFI GGCC 9 cut(s) 316, 388, 2580, 3197, 3243, 3661, 3846, 3933, 4943
BshNI GGYRCC 1 cut(s) 4247
BshTI ACCGGT 1 cut(s) 4151
BsiEI CGRYCG 4 cut(s) 129, 255, 317, 372
BsiHKAI GWGCWC 6 cut(s) 48, 2769, 3100, 3355, 4479, 4561
BsiHKCI CYCGRG 1 cut(s) 431
BsiWI CGTACG 1 cut(s) 122
BslFI GGGAC 3 cut(s) 98, 1361, 3488
BslI CCNNNNNNNGG 7 cut(s) 522, 2503, 3031, 3263, 3853, 5211, 5641
BsmAI GTCTC 8 cut(s) 1189, 2220, 2886, 3736, 3860, 4339, 4445, 4645
BsmFI GGGAC 3 cut(s) 98, 1361, 3488
BsmI GAATGC 4 cut(s) 427, 631, 2702, 4124
BsnI GGCC 9 cut(s) 316, 388, 2580, 3197, 3243, 3661, 3846, 3933, 4943
Bso31I GGTCTC 3 cut(s) 2220, 4339, 4645
BsoBI CYCGRG 1 cut(s) 431
Bsp119I TTCGAA 3 cut(s) 635, 884, 5124
Bsp1286I GDGCHC 6 cut(s) 48, 2769, 3100, 3355, 4479, 4561
Bsp1407I TGTACA 3 cut(s) 330, 1597, 2623
Bsp1720I GCTNAGC 1 cut(s) 5108
Bsp19I CCATGG 2 cut(s) 39, 2498
BspANI GGCC 9 cut(s) 316, 388, 2580, 3197, 3243, 3661, 3846, 3933, 4943
BspFNI CGCG 7 cut(s) 187, 215, 446, 491, 1267, 3078, 4470
BspMAI CTGCAG 1 cut(s) 3519
BspMI ACCTGC 3 cut(s) 703, 1883, 3826
BspOI GCTAGC 1 cut(s) 3630
BspQI GCTCTTC 2 cut(s) 1816, 3715
BspT104I TTCGAA 3 cut(s) 635, 884, 5124
BspT107I GGYRCC 1 cut(s) 4247
BspTI CTTAAG 1 cut(s) 2743
BspTNI GGTCTC 3 cut(s) 2220, 4339, 4645
BsrBI CCGCTC 3 cut(s) 278, 522, 1450
BsrDI GCAATG 7 cut(s) 607, 751, 1338, 1842, 2568, 3023, 4681
BsrFI RCCGGY 1 cut(s) 4151
BsrGI TGTACA 3 cut(s) 330, 1597, 2623
BsrI ACTGG 9 cut(s) 258, 919, 1068, 1380, 1592, 1660, 2287, 2543, 4417
BssAI RCCGGY 1 cut(s) 4151
BssNI GRCGYC 3 cut(s) 243, 479, 623
BssSI CACGAG 1 cut(s) 4898
BssT1I CCWWGG 4 cut(s) 39, 1318, 2498, 3199
Bst2BI CACGAG 1 cut(s) 4898
Bst2UI CCWGG 7 cut(s) 160, 2648, 3258, 3923, 3971, 5069, 5670
BstACI GRCGYC 3 cut(s) 243, 479, 623
BstAFI CTTAAG 1 cut(s) 2743
BstAPI GCANNNNNTGC 1 cut(s) 1559
BstAUI TGTACA 3 cut(s) 330, 1597, 2623
BstBAI YACGTR 1 cut(s) 1637
BstBI TTCGAA 3 cut(s) 635, 884, 5124
BstDSI CCRYGG 4 cut(s) 39, 395, 489, 2498
BstEII GGTNACC 2 cut(s) 3138, 4876
BstENI CCTNNNNNAGG 1 cut(s) 3851
BstFNI CGCG 7 cut(s) 187, 215, 446, 491, 1267, 3078, 4470
BstH2I RGCGCY 2 cut(s) 443, 516
BstHHI GCGC 2 cut(s) 442, 515
BstMAI GTCTC 8 cut(s) 1189, 2220, 2886, 3736, 3860, 4339, 4445, 4645
BstMCI CGRYCG 4 cut(s) 129, 255, 317, 372
BstNI CCWGG 7 cut(s) 160, 2648, 3258, 3923, 3971, 5069, 5670
BstNSI RCATGY 7 cut(s) 2000, 2938, 3705, 3717, 4724, 4728, 5602
BstPI GGTNACC 2 cut(s) 3138, 4876
BstSFI CTRYAG 1 cut(s) 3515
BstSLI GKGCMC 1 cut(s) 2769
BstUI CGCG 7 cut(s) 187, 215, 446, 491, 1267, 3078, 4470
BstV2I GAAGAC 1 cut(s) 3743
BstX2I RGATCY 4 cut(s) 1495, 1916, 2859, 3620
BstXI CCANNNNNNTGG 7 cut(s) 1228, 2239, 2588, 3617, 3929, 4888, 5075
BstYI RGATCY 4 cut(s) 1495, 1916, 2859, 3620
BstZI CGGCCG 1 cut(s) 314
BsuI GTATCC 4 cut(s) 1226, 1505, 3197, 5134
BsuRI GGCC 9 cut(s) 316, 388, 2580, 3197, 3243, 3661, 3846, 3933, 4943
BtgI CCRYGG 4 cut(s) 39, 395, 489, 2498
BtsI GCAGTG 3 cut(s) 3637, 5509, 5689
BtsIMutI CAGTG 9 cut(s) 265, 789, 1739, 2769, 3637, 4230, 4477, 5509, 5689
BveI ACCTGC 3 cut(s) 703, 1883, 3826
CaiI CAGNNNCTG 2 cut(s) 1655, 2263
CfoI GCGC 2 cut(s) 442, 515
Cfr10I RCCGGY 1 cut(s) 4151
Cfr42I CCGCGG 1 cut(s) 492
Cfr9I CCCGGG 1 cut(s) 431
CpoI CGGWCCG 1 cut(s) 310
CseI GACGC 5 cut(s) 204, 251, 487, 631, 1774
CsiI ACCWGGT 2 cut(s) 2646, 3921
CspAI ACCGGT 1 cut(s) 4151
CspCI CAANNNNNGTGG 2 cut(s) 4335, 4370
CspI CGGWCCG 1 cut(s) 310
DraI TTTAAA 1 cut(s) 4269
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 2 cut(s) 314, 2578
EagI CGGCCG 1 cut(s) 314
Ecl136II GAGCTC 1 cut(s) 46
EclXI CGGCCG 1 cut(s) 314
Eco130I CCWWGG 4 cut(s) 39, 1318, 2498, 3199
Eco147I AGGCCT 1 cut(s) 3243
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 3 cut(s) 2220, 4339, 4645
Eco32I GATATC 2 cut(s) 3037, 5538
Eco47I GGWCC 9 cut(s) 310, 345, 579, 842, 1218, 2465, 3559, 4141, 5654
Eco52I CGGCCG 1 cut(s) 314
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 2136, 3438
Eco88I CYCGRG 1 cut(s) 431
Eco91I GGTNACC 2 cut(s) 3138, 4876
EcoICRI GAGCTC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 3851
EcoO65I GGTNACC 2 cut(s) 3138, 4876
EcoRI GAATTC 5 cut(s) 769, 2816, 3365, 3877, 5133
EcoRII CCWGG 7 cut(s) 158, 2646, 3256, 3921, 3969, 5067, 5668
EcoRV GATATC 2 cut(s) 3037, 5538
EcoT14I CCWWGG 4 cut(s) 39, 1318, 2498, 3199
EcoT22I ATGCAT 2 cut(s) 1018, 4726
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 4 cut(s) 39, 1318, 2498, 3199
FalI AAGNNNNNCTT 6 cut(s) 2431, 2463, 3218, 3250, 5491, 5523
FaqI GGGAC 3 cut(s) 98, 1361, 3488
FauI CCCGC 4 cut(s) 192, 271, 515, 2958
FauNDI CATATG 3 cut(s) 2668, 5116, 5243
FbaI TGATCA 4 cut(s) 994, 1297, 2254, 3679
FriOI GRGCYC 1 cut(s) 48
GlaI GCGC 2 cut(s) 441, 514
GsuI CTGGAG 4 cut(s) 1085, 1575, 3822, 4424
HaeII RGCGCY 2 cut(s) 443, 516
HaeIII GGCC 9 cut(s) 316, 388, 2580, 3197, 3243, 3661, 3846, 3933, 4943
HgaI GACGC 5 cut(s) 204, 251, 487, 631, 1774
HhaI GCGC 2 cut(s) 442, 515
Hin1I GRCGYC 3 cut(s) 243, 479, 623
Hin6I GCGC 2 cut(s) 440, 513
HinP1I GCGC 2 cut(s) 440, 513
HincII GTYRAC 4 cut(s) 2599, 3637, 4044, 5556
HindII GTYRAC 4 cut(s) 2599, 3637, 4044, 5556
HindIII AAGCTT 3 cut(s) 1146, 2415, 4601
Hpy99I CGWCG 7 cut(s) 134, 245, 394, 481, 625, 631, 1768
HpyCH4IV ACGT 2 cut(s) 1636, 3791
HpySE526I ACGT 2 cut(s) 1636, 3791
Hsp92I GRCGYC 3 cut(s) 243, 479, 623
HspAI GCGC 2 cut(s) 440, 513
Ksp22I TGATCA 4 cut(s) 994, 1297, 2254, 3679
KspI CCGCGG 1 cut(s) 492
LguI GCTCTTC 2 cut(s) 1816, 3715
MabI ACCWGGT 2 cut(s) 2646, 3921
MaeII ACGT 2 cut(s) 1636, 3791
MbiI CCGCTC 3 cut(s) 278, 522, 1450
MfeI CAATTG 7 cut(s) 293, 2075, 2145, 2425, 3570, 5293, 5606
MflI RGATCY 4 cut(s) 1495, 1916, 2859, 3620
MhlI GDGCHC 6 cut(s) 48, 2769, 3100, 3355, 4479, 4561
MlsI TGGCCA 1 cut(s) 2580
MluI ACGCGT 1 cut(s) 1265
MluNI TGGCCA 1 cut(s) 2580
MlyI GAGTC 7 cut(s) 50, 83, 298, 727, 3470, 3632, 4796
MmeI TCCRAC 9 cut(s) 111, 117, 1877, 2229, 3812, 4512, 4531, 4597, 5054
Mox20I TGGCCA 1 cut(s) 2580
Mph1103I ATGCAT 2 cut(s) 1018, 4726
MroXI GAANNNNTTC 4 cut(s) 1904, 2511, 3131, 4002
MscI TGGCCA 1 cut(s) 2580
Msp20I TGGCCA 1 cut(s) 2580
MspA1I CMGCKG 3 cut(s) 491, 3497, 5627
MspCI CTTAAG 1 cut(s) 2743
MunI CAATTG 7 cut(s) 293, 2075, 2145, 2425, 3570, 5293, 5606
Mva1269I GAATGC 4 cut(s) 427, 631, 2702, 4124
MvaI CCWGG 7 cut(s) 160, 2648, 3258, 3923, 3971, 5069, 5670
MvnI CGCG 7 cut(s) 187, 215, 446, 491, 1267, 3078, 4470
NciI CCSGG 8 cut(s) 318, 338, 349, 359, 432, 433, 517, 2488
NcoI CCATGG 2 cut(s) 39, 2498
NdeI CATATG 3 cut(s) 2668, 5116, 5243
NheI GCTAGC 1 cut(s) 3626
NmeAIII GCCGAG 1 cut(s) 1150
NmuCI GTSAC 7 cut(s) 1523, 1630, 1951, 2534, 3138, 4375, 5664
NsiI ATGCAT 2 cut(s) 1018, 4726
NspI RCATGY 7 cut(s) 2000, 2938, 3705, 3717, 4724, 4728, 5602
NspV TTCGAA 3 cut(s) 635, 884, 5124
PaeI GCATGC 1 cut(s) 4724
PaqCI CACCTGC 1 cut(s) 703
PceI AGGCCT 1 cut(s) 3243
PciSI GCTCTTC 2 cut(s) 1816, 3715
PctI GAATGC 4 cut(s) 427, 631, 2702, 4124
PdmI GAANNNNTTC 4 cut(s) 1904, 2511, 3131, 4002
Pfl23II CGTACG 1 cut(s) 122
PflFI GACNNNGTC 2 cut(s) 625, 1768
PfoI TCCNGGA 1 cut(s) 357
PinAI ACCGGT 1 cut(s) 4151
Ple19I CGATCG 1 cut(s) 129
PleI GAGTC 7 cut(s) 50, 83, 297, 726, 3470, 3632, 4796
PpsI GAGTC 7 cut(s) 50, 83, 297, 726, 3470, 3632, 4796
Ppu21I YACGTR 1 cut(s) 1637
PshBI ATTAAT 1 cut(s) 4371
PsiI TTATAA 2 cut(s) 1398, 2421
Psp124BI GAGCTC 1 cut(s) 48
Psp6I CCWGG 7 cut(s) 158, 2646, 3256, 3921, 3969, 5067, 5668
PspEI GGTNACC 2 cut(s) 3138, 4876
PspGI CCWGG 7 cut(s) 158, 2646, 3256, 3921, 3969, 5067, 5668
PspLI CGTACG 1 cut(s) 122
PsrI GAACNNNNNNTAC 2 cut(s) 5452, 5484
PstI CTGCAG 1 cut(s) 3519
PstNI CAGNNNCTG 2 cut(s) 1655, 2263
PsuI RGATCY 4 cut(s) 1495, 1916, 2859, 3620
PsyI GACNNNGTC 2 cut(s) 625, 1768
PvuI CGATCG 1 cut(s) 129
PvuII CAGCTG 2 cut(s) 3497, 5627
Rsr2I CGGWCCG 1 cut(s) 310
RsrII CGGWCCG 1 cut(s) 310
SacI GAGCTC 1 cut(s) 48
SacII CCGCGG 1 cut(s) 492
SapI GCTCTTC 2 cut(s) 1816, 3715
ScaI AGTACT 2 cut(s) 2761, 3230
SchI GAGTC 7 cut(s) 50, 83, 298, 727, 3470, 3632, 4796
SduI GDGCHC 6 cut(s) 48, 2769, 3100, 3355, 4479, 4561
SexAI ACCWGGT 2 cut(s) 2646, 3921
SfcI CTRYAG 1 cut(s) 3515
Sfr303I CCGCGG 1 cut(s) 492
SfuI TTCGAA 3 cut(s) 635, 884, 5124
SgrBI CCGCGG 1 cut(s) 492
SinI GGWCC 9 cut(s) 310, 345, 579, 842, 1218, 2465, 3559, 4141, 5654
SmaI CCCGGG 1 cut(s) 433
SmlI CTYRAG 7 cut(s) 1199, 1311, 2743, 3358, 3851, 4669, 5192
SmoI CTYRAG 7 cut(s) 1199, 1311, 2743, 3358, 3851, 4669, 5192
SphI GCATGC 1 cut(s) 4724
SseBI AGGCCT 1 cut(s) 3243
SspI AATATT 1 cut(s) 5097
SstI GAGCTC 1 cut(s) 48
StuI AGGCCT 1 cut(s) 3243
StyI CCWWGG 4 cut(s) 39, 1318, 2498, 3199
TaiI ACGT 2 cut(s) 1639, 3794
TaqII GACCGA 2 cut(s) 241, 358
TauI GCSGC 9 cut(s) 440, 449, 452, 458, 461, 484, 491, 494, 3078
TscAI CASTG 9 cut(s) 265, 796, 1739, 2776, 3637, 4230, 4484, 5509, 5689
TseFI GTSAC 7 cut(s) 1523, 1630, 1951, 2534, 3138, 4375, 5664
Tsp45I GTSAC 7 cut(s) 1523, 1630, 1951, 2534, 3138, 4375, 5664
TspGWI ACGGA 5 cut(s) 100, 2524, 2539, 4759, 5667
TspMI CCCGGG 1 cut(s) 431
TspRI CASTG 9 cut(s) 265, 796, 1739, 2776, 3637, 4230, 4484, 5509, 5689
Tth111I GACNNNGTC 2 cut(s) 625, 1768
Vha464I CTTAAG 1 cut(s) 2743
VneI GTGCAC 1 cut(s) 2765
VpaK11BI GGWCC 9 cut(s) 310, 345, 579, 842, 1218, 2465, 3559, 4141, 5654
VspI ATTAAT 1 cut(s) 4371
XagI CCTNNNNNAGG 1 cut(s) 3851
XbaI TCTAGA 3 cut(s) 1741, 2341, 4085
XceI RCATGY 7 cut(s) 2000, 2938, 3705, 3717, 4724, 4728, 5602
XcmI CCANNNNNNNNNTGG 1 cut(s) 5471
XmaI CCCGGG 1 cut(s) 431
XmnI GAANNNNTTC 4 cut(s) 1904, 2511, 3131, 4002
ZrmI AGTACT 2 cut(s) 2761, 3230
Zsp2I ATGCAT 2 cut(s) 1018, 4726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.