Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0006617

Physical Location & Seq

Genomic Coordinates Forward (+)

53274326 .. 53283967

Gene Structure 9642 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G026210.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3771 bp

ATGCCTTGTACTGCTTGGAAATGCACAACTCAATTGCATAATTCACCTACTAATAGTGAGAATACGCCTACTGAAGCAGTTGAAAGAGTTTCATTACTTGCAATTGCTTGGGATCGGAAAGTTCAGGTAGCAAAGTTGGTCAAATCAGAGCTGAAAGTATATGGTAAATGGTCTCTTGAAAGTGCAGCCATAGGTGTGGCTTGGTTGGATGATCAGATGCTGGTGGTTCTCACGATAACTGGACAACTTTGTTTGTTTGCAAAGGATGGAACTGTAATTCACCAAACAAGTTTTTCTCTAGATGGTTTTAGAGGGGATGATCTCATTTCTTATCATACTCACTTCATCAATGTATTTGGAAACCCTGAAAAAGCTTATAACAATTGCATAGCTGTGAGAGGAGCTTCTGTATATGTACTTGGACCTACTCATCTTATTGTTTCCCGGCTTCTCCCATGGAAGGAACGGATTCAGGTTTTACGGAGAGCTGGTGACTGGATGGGTTCTTTGAACATGGCAATGACAATTTATGATGGCCAAGCTCATGGTGTTGTTGACCTTCCTAGAACTCTGGATGCTGTACAGGAGGTCATAATGCCCTACCTGGTTGAGTTGCTTTTGTCATATGTAGAGGAAGTTTTTTCTTATATTTCAGTAGCATTCTGCAACCAAATTGAAAAAATGGATCAAGTTGATGACTTAAACAGACAAAGTAGTACTGTGCACACTGAAATTAAAGAGCAATACACCCGTGTTGGTGGGGTTGCAGTTGAATTCTGTGTCCATATCAAGAGGACTGACATACTTTTTGATGAGATCTTCTTGAAGTTTGTAGCTGTTCAACAGAGAGACACATTTTTGGAACTATTGGAGCCTTATATATTGAAGGACATGCTTGGTTCTCTTCCACCTGAGATTATGCAAGCTCTGGTAGAACATTACAGCCACAAAGGGTGGTTACAGCGTGTTGAGCAATGTGTCCTTCACATGGATATTTCTTCATTGGATTTCAATCAGGTTGTTAGGTTATGCCGCGAGCATGGATTATATAGTGCCCTGGTGTATCTTTTCAACAAAGGATTGAATGATTTCAGGTCACCATTAGAGGAGCTGTTGGTGGTCTTGCGAAATAGTCAAAAAGAAGGTGCTATGGCCCTTGGATACAGGATGCTAGTTTATTTGAAGTACTGCTTTTCAGGCCTTGCTTTTCCACCAGGACAAGGAAAAATTCCTCCTTCACGCTTGCCATCTCTCAGAACAGAACTTTTGCAGTTTTTGTTGGAGGGTTCTGATGCCCCTAATTCAAGAGCACTCTCAAGCGAATTCCCAGGAGGAGAATATCTCAACCTTTATTATCTCTTAGAACTAGATACTGAAGCTACTTTAGATGTTCTGAGATGTGCTTTCACAGAAGTTGAAATCTCGAAATGGGACTTCTCTTCTCAAAATTCAGCTGATGCAGACACAGAGCTGCAGTATGGGAATAATTCAACGGCTCAAAGTCAGGATTCATTGGTCCAGAATACAATTGATACTCTTATTCACATTATTAGTAAGGACATTCCCCAGAAGGATGGATCTGCTAGCAGTGTTGACTCTGGATCAGTTGTAGAGTGGCCTTCAAAGAAAGATATTGATCATCTATTCGAGTTTATTGCATATTATGTTGCATGTGGAAAAGCTAATGTCTCGAAGACTGTACTTTCTCAGATTTTGGAGTATTTGACATCACAGAATAACTTTCCAACGTCTGTTTCTGGGGATAGCATAATTTCAAAAAGAAGAGAGAAGCAGGTTCTTGGCCTTCTTGAGGTAGTGCCGGAGACTGATTGGAATTCATCTTCTGTGTTACAGCTATGTGAGAAAGCACAATTTTACCAGGTATGTGGCCTAATTCATACCAGCAGACATCAGTATATTGCTGCCCTGGATTGCTATATGAAAGATGCAGCCGAACCCATTCATGCCTTTGCCTTTATCAACAAAACATTGTTACAGTTGACTGACGATGAATGTGCTGCTTTTCAATCAGCAGTTATTTCTAGAATTCCAGAGCTATTTAACTTAAACAGAGAGGGTGCATTCTTCTTGGTCATGGACCATTTTACCAGTGCAGAAGGTTCTCATATCCTATCTGAACTTCGCTCACATCCACGAAGCCTTTTCCTGTACTTAAAAACAGTGATTGAGGTTCATTTATCTGGCACCCTCGACTTCTCAAGTTTAAAAACCGACAATCTTATGGGAGTAAAGGATCAAACAAAAGGAGTTGAGGCTTTCTTAGAAAGGGTCTCTAATTTCCCACAGCTTTTGCGTGATAGTCCTATTAATGTGACTGATGATATGATTGAGCTTTATCTGGAGCTGTTATGCCAGTTTGAATGCAAATCAGTTCTCAAATTCCTAGAGACATTTGATAGCTATCGTGTGGAGCACTGTTTACGCCTCTGCCAGAAATATGCAATAATAGATGCTTCATCATTCTTGTTGGAGAGGGTTGGTGATGTTGGAAGTGCACTTTTACTTACACTTTCCACCCTCAATGACAAATTTATCAAGCTTGAAACTGCTGTTGGAAGTCTAAATTTAAGTGGGGCTTCACGTGGTTCTGCTAGTACAGAATGCTTGAGCAATGCTTTGAAAGTGGAGGAGGTGAATGATATAGACAGTATATTGCATGCATGTATAGGATTGTGCCAACGGAACACCCATCGTCTAAATCCTGATGAATCAGAGGCACTCTGGTTCAAATTGCTTGACTCGTTTTGTGAGCCTTTAATGGATTCGTTTAGTGCTGGTACAGTATCTAAAGGAGAAGATCATAACAGAATGGTAACCAATTCATTGGATTCACAAGAGGATGAAATGAATTTCACAATCAAGTGGAGAATCCCAAAGGCTCATAAAGGTGCTGATATTTTAAGAAAATTGTTTTCTCGGTTCATCAAGGAGATTGTTGAGGGCATGATAGGATATGTTCGCCTTCCAACTATCATGTCAAAGCTCCTTTCTGACAATGGTAGCCAGGAATTTGGTGATTTTAAATTCACAATATTGGGGATGCTTAGCACATATGGCTTTGAAAGAAGAATTCTGGATACTGCCAAGTCCCTGATAGAAGATGATACATTCTATACTATGAGCATCCTCAAGAAAGGAGCCTCACATGGGTATGCTCCTCGCAACCCAAAATGTTGCATATGCGATTGCCTCCTTGACAAGAACTCCTCCAGCTACATTCGCATTTTCAATTGTGGTCATGCAACACATCTTAAATGCGAAGTTTCAGAGAATGAAACATCAAGTAGTAGCTCGTCCTCTGGATGCCCAGTTTGTATGCCCAAGAAGAAATCTCAGAGGTCTAAAAACAAATCGGTTCTAGCAGAGGAGAGTTTAGTGAAGGTGTTTTCATCAAGAACCCATCATAGTCATGGAACAGTTGTTCACCCACATGAAAGCACTTCTGAGAATAGCTATGGGATTCAGCAGATATCACGATTTGAGATGTTGACCAATTTGCAGAAACATCGTGGATTAGTCGAGATTGAAAACATGCCTCAATTGAGGCTTGCCCCACCAGCTGTGTACCATGAAAAGGTTAAGCACGGACCTGTTCTGTCACCAGGAGAGAGCAGCAGTGATCTTGTGAGAACAGAGAAACAAAGTAAAATCAAGCAACTCAGGGAGGTAAAAGTAAAAGGATCATCTCTTCGGTTTCCCTTGAAAACAAACTTATTTGGTAATGGCAAGGACAAGATCAGCAGGCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0006810 GO:0006906 GO:0006996 GO:0008150 GO:0009987 GO:0012505 GO:0016043 GO:0016050 GO:0016192 GO:0031410 GO:0031982 GO:0032991 GO:0033263 GO:0034058 GO:0043226 GO:0043227 GO:0043229 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0048284 GO:0051179 GO:0051234 GO:0061024 GO:0061025 GO:0071840 GO:0090174 GO:0097708 GO:0099023

KEGG Pathways

Metabolic & Signaling

ko04138 map04138

Pfam Domains

Protein Families

ANAPC4_WD40 Clathrin Vps8

Protein Analysis

1256

Amino Acids

141.18

Weight (kDa)

5.9

Isoelectric Point (pI)

43.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_Vps8	PF12816	286 - 469	3.2e-51	Vps8 central TPR
TPR_Vps41	PF23556	781 - 849	7.6e-07	Vps41 TPR-like region
TPR_VPS8_2	PF25066	901 - 1048	1.2e-06	VPS8-like, TPR-like repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006617)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00800 AT4G00800
fragaria_vesca	FvH4_3g34060 FvH4_7g01070 FvH4_7g17640 FvH4_7g17640 FvH4_7g17640 FvH4_7g17640 FvH4_7g17640 FvH4_7g17640
malus_domestica	MD07G1159400.v1.1
prunus_persica	Prupe.2G197500_v2.0.a1
pyrus_communis	pycom01g11250 pycom07g15600
rosa_chinensis	RchiOBHm_Chr1g0361071
rosa_laevigata	RLG00000027750
rosa_multiflora	Rmu_sc0003333.1_g000009 Rmu_sc0014603.1_g000002 Rmu_sc0024329.1_g000001
rosa_roxburghii	Rroxscaffold_4G00295450
rosa_rugosa	Rorug01G0285300
rosa_samantha	Rh1AG296700 Rh1BG261000 Rh1CG278300 Rh1DG291000
rosa_wichuraiana	Rw1G026210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	378
Acc36I	ACCTGC	1 cut(s)	1783
AccB1I	GGYRCC	1 cut(s)	2204
AccII	CGCG	1 cut(s)	1035
AciI	CCGC	1 cut(s)	1033
AclWI	GGATC	6 cut(s)	120, 693, 1585, 1609, 2262, 3709
AcoI	YGGCCR	1 cut(s)	535
AcuI	CTGAAG	2 cut(s)	93, 1395
AcvI	CACGTG	1 cut(s)	2603
AfiI	CCNNNNNNNGG	6 cut(s)	460, 988, 1220, 1810, 3168, 3595
AjnI	CCWGG	8 cut(s)	603, 1056, 1213, 1327, 1878, 1926, 3024, 3622
AjuI	GAANNNNNNNTTGG	4 cut(s)	1738, 1770, 2556, 2588
AloI	GAACNNNNNNTCC	2 cut(s)	3426, 3458
Alw21I	GWGCWC	4 cut(s)	726, 1312, 2436, 2518
Alw26I	GTCTC	6 cut(s)	177, 843, 1693, 1817, 2296, 2402
Alw44I	GTGCAC	2 cut(s)	722, 2514
AlwI	GGATC	6 cut(s)	120, 693, 1585, 1609, 2262, 3709
AlwNI	CAGNNNCTG	1 cut(s)	220
AoxI	GGCC	6 cut(s)	535, 1152, 1198, 1616, 1801, 1888
ApaLI	GTGCAC	2 cut(s)	722, 2514
ApeKI	GCWGC	6 cut(s)	185, 1471, 1922, 1949, 2018, 3633
ArsI	GACNNNNNNTTYG	2 cut(s)	791, 823
AseI	ATTAAT	1 cut(s)	2328
Asp700I	GAANNNNTTC	3 cut(s)	468, 1088, 1959
AspS9I	GGNCC	5 cut(s)	422, 1153, 1516, 2098, 3608
AsuC2I	CCSGG	1 cut(s)	445
AsuHPI	GGTGA	9 cut(s)	36, 272, 503, 1089, 2513, 2665, 3047, 3437, 3612
AsuNHI	GCTAGC	1 cut(s)	1583
AvaII	GGWCC	4 cut(s)	422, 1516, 2098, 3608
BaeGI	GKGCMC	3 cut(s)	726, 1057, 2518
BalI	TGGCCA	1 cut(s)	537
BanI	GGYRCC	1 cut(s)	2204
BbrPI	CACGTG	1 cut(s)	2603
BbsI	GAAGAC	1 cut(s)	1700
Bbv12I	GWGCWC	4 cut(s)	726, 1312, 2436, 2518
BbvI	GCAGC	6 cut(s)	197, 1458, 1909, 1961, 2005, 3645
BccI	CCATC	8 cut(s)	260, 296, 493, 527, 1255, 1568, 2718, 3429
BceAI	ACGGC	1 cut(s)	1509
BcgI	CGANNNNNNTGC	6 cut(s)	1637, 1671, 1997, 2031, 3509, 3543
BciT130I	CCWGG	8 cut(s)	605, 1058, 1215, 1329, 1880, 1928, 3026, 3624
BciVI	GTATCC	2 cut(s)	1154, 3091
BclI	TGATCA	2 cut(s)	211, 1636
BcnI	CCSGG	1 cut(s)	445
BcoDI	GTCTC	6 cut(s)	177, 843, 1693, 1817, 2296, 2402
BfaI	CTAG	9 cut(s)	299, 564, 1172, 1367, 1584, 2043, 2405, 2613, 3380
BfmI	CTRYAG	1 cut(s)	1472
BfuAI	ACCTGC	1 cut(s)	1783
BfuI	GTATCC	2 cut(s)	1154, 3091
BglII	AGATCT	1 cut(s)	816
BisI	GCNGC	7 cut(s)	186, 1033, 1472, 1923, 1950, 2019, 3634
BlpI	GCTNAGC	1 cut(s)	3065
BlsI	GCNGC	7 cut(s)	187, 1034, 1473, 1924, 1951, 2020, 3635
BmcAI	AGTACT	2 cut(s)	718, 1187
Bme1390I	CCNGG	9 cut(s)	445, 605, 1058, 1215, 1329, 1880, 1928, 3026, 3624
Bme18I	GGWCC	4 cut(s)	422, 1516, 2098, 3608
BmgT120I	GGNCC	5 cut(s)	422, 1153, 1516, 2098, 3608
BmiI	GGNNCC	3 cut(s)	873, 2206, 3160
BmrFI	CCNGG	9 cut(s)	445, 605, 1058, 1215, 1329, 1880, 1928, 3026, 3624
BmrI	ACTGGG	1 cut(s)	3323
BmtI	GCTAGC	1 cut(s)	1587
BmuI	ACTGGG	1 cut(s)	3323
BpiI	GAAGAC	1 cut(s)	1700
BplI	GAGNNNNNCTC	2 cut(s)	1326, 1358
BpmI	CTGGAG	2 cut(s)	2381, 3214
Bpu1102I	GCTNAGC	1 cut(s)	3065
BpuEI	CTTGAG	5 cut(s)	1300, 1829, 2203, 2647, 3134
BpuMI	CCSGG	1 cut(s)	445
BsaAI	YACGTR	1 cut(s)	2603
BsaBI	GATNNNNATC	3 cut(s)	1011, 1635, 2421
BsaI	GGTCTC	2 cut(s)	177, 2296
BsaJI	CCNNGG	5 cut(s)	455, 1056, 1156, 1327, 1926
BsaXI	ACNNNNNCTCC	6 cut(s)	393, 423, 475, 505, 3209, 3239
Bsc4I	CCNNNNNNNGG	6 cut(s)	460, 988, 1220, 1810, 3168, 3595
Bse1I	ACTGG	5 cut(s)	244, 500, 2109, 2374, 3329
Bse3DI	GCAATG	3 cut(s)	525, 980, 2638
Bse8I	GATNNNNATC	3 cut(s)	1011, 1635, 2421
BseBI	CCWGG	8 cut(s)	605, 1058, 1215, 1329, 1880, 1928, 3026, 3624
BseDI	CCNNGG	5 cut(s)	455, 1056, 1156, 1327, 1926
BseJI	GATNNNNATC	3 cut(s)	1011, 1635, 2421
BseLI	CCNNNNNNNGG	6 cut(s)	460, 988, 1220, 1810, 3168, 3595
BseMI	GCAATG	3 cut(s)	525, 980, 2638
BseMII	CTCAG	7 cut(s)	903, 1267, 1385, 1721, 3368, 3456, 3694
BseNI	ACTGG	5 cut(s)	244, 500, 2109, 2374, 3329
BseRI	GAGGAG	7 cut(s)	414, 1121, 1347, 2663, 3168, 3217, 3401
BseSI	GKGCMC	3 cut(s)	726, 1057, 2518
BseXI	GCAGC	6 cut(s)	197, 1458, 1909, 1961, 2005, 3645
BsgI	GTGCAG	2 cut(s)	204, 2133
Bsh1236I	CGCG	1 cut(s)	1035
BshFI	GGCC	6 cut(s)	537, 1154, 1200, 1618, 1803, 1890
BshNI	GGYRCC	1 cut(s)	2204
BsiHKAI	GWGCWC	4 cut(s)	726, 1312, 2436, 2518
BsiSI	CCGG	2 cut(s)	445, 1820
BslFI	GGGAC	2 cut(s)	1445, 3094
BslI	CCNNNNNNNGG	6 cut(s)	460, 988, 1220, 1810, 3168, 3595
BsmAI	GTCTC	6 cut(s)	177, 843, 1693, 1817, 2296, 2402
BsmFI	GGGAC	2 cut(s)	1445, 3094
BsmI	GAATGC	4 cut(s)	659, 2081, 2387, 2627
BsnI	GGCC	6 cut(s)	537, 1154, 1200, 1618, 1803, 1890
Bso31I	GGTCTC	2 cut(s)	177, 2296
Bsp1286I	GDGCHC	5 cut(s)	726, 1057, 1312, 2436, 2518
Bsp1407I	TGTACA	1 cut(s)	580
Bsp1720I	GCTNAGC	1 cut(s)	3065
Bsp19I	CCATGG	1 cut(s)	455
BspACI	CCGC	1 cut(s)	1033
BspANI	GGCC	6 cut(s)	537, 1154, 1200, 1618, 1803, 1890
BspCNI	CTCAG	7 cut(s)	904, 1266, 1386, 1720, 3367, 3457, 3693
BspFNI	CGCG	1 cut(s)	1035
BspLI	GGNNCC	3 cut(s)	873, 2206, 3160
BspMAI	CTGCAG	1 cut(s)	1476
BspMI	ACCTGC	1 cut(s)	1783
BspOI	GCTAGC	1 cut(s)	1587
BspPI	GGATC	6 cut(s)	120, 693, 1585, 1609, 2262, 3709
BspT107I	GGYRCC	1 cut(s)	2204
BspTNI	GGTCTC	2 cut(s)	177, 2296
BsrDI	GCAATG	3 cut(s)	525, 980, 2638
BsrGI	TGTACA	1 cut(s)	580
BsrI	ACTGG	5 cut(s)	244, 500, 2109, 2374, 3329
BssECI	CCNNGG	5 cut(s)	455, 1056, 1156, 1327, 1926
BssT1I	CCWWGG	2 cut(s)	455, 1156
Bst2UI	CCWGG	8 cut(s)	605, 1058, 1215, 1329, 1880, 1928, 3026, 3624
Bst4CI	ACNGT	9 cut(s)	274, 721, 1699, 1998, 2182, 2438, 2669, 2803, 3439
Bst6I	CTCTTC	4 cut(s)	909, 1444, 1777, 3714
BstAUI	TGTACA	1 cut(s)	580
BstBAI	YACGTR	1 cut(s)	2603
BstC8I	GCNNGC	7 cut(s)	924, 1037, 1244, 1585, 2679, 3570, 3764
BstDSI	CCRYGG	1 cut(s)	455
BstEII	GGTNACC	2 cut(s)	1095, 2833
BstENI	CCTNNNNNAGG	1 cut(s)	1808
BstFNI	CGCG	1 cut(s)	1035
BstMAI	GTCTC	6 cut(s)	177, 843, 1693, 1817, 2296, 2402
BstMWI	GCNNNNNNNGC	5 cut(s)	970, 1197, 3075, 3240, 3578
BstNI	CCWGG	8 cut(s)	605, 1058, 1215, 1329, 1880, 1928, 3026, 3624
BstNSI	RCATGY	5 cut(s)	895, 1674, 2681, 2685, 3556
BstPI	GGTNACC	2 cut(s)	1095, 2833
BstSCI	CCNGG	9 cut(s)	443, 603, 1056, 1213, 1327, 1878, 1926, 3024, 3622
BstSFI	CTRYAG	1 cut(s)	1472
BstSLI	GKGCMC	3 cut(s)	726, 1057, 2518
BstUI	CGCG	1 cut(s)	1035
BstV1I	GCAGC	6 cut(s)	197, 1458, 1909, 1961, 2005, 3645
BstV2I	GAAGAC	1 cut(s)	1700
BstX2I	RGATCY	2 cut(s)	816, 1577
BstXI	CCANNNNNNTGG	6 cut(s)	196, 545, 1574, 1886, 2845, 3032
BstYI	RGATCY	2 cut(s)	816, 1577
BsuI	GTATCC	2 cut(s)	1154, 3091
BsuRI	GGCC	6 cut(s)	537, 1154, 1200, 1618, 1803, 1890
BtgI	CCRYGG	1 cut(s)	455
BtsI	GCAGTG	2 cut(s)	1594, 3643
BtsIMutI	CAGTG	6 cut(s)	726, 1594, 2116, 2187, 2434, 3643
BveI	ACCTGC	1 cut(s)	1783
Cac8I	GCNNGC	7 cut(s)	924, 1037, 1244, 1585, 2679, 3570, 3764
CaiI	CAGNNNCTG	1 cut(s)	220
Cfr13I	GGNCC	5 cut(s)	422, 1153, 1516, 2098, 3608
CsiI	ACCWGGT	2 cut(s)	603, 1878
CspCI	CAANNNNNGTGG	2 cut(s)	2292, 2327
DraI	TTTAAA	2 cut(s)	2226, 3043
EaeI	YGGCCR	1 cut(s)	535
Eam1104I	CTCTTC	4 cut(s)	909, 1444, 1777, 3714
EarI	CTCTTC	4 cut(s)	909, 1444, 1777, 3714
Eco130I	CCWWGG	2 cut(s)	455, 1156
Eco147I	AGGCCT	1 cut(s)	1200
Eco31I	GGTCTC	2 cut(s)	177, 2296
Eco32I	GATATC	1 cut(s)	3492
Eco47I	GGWCC	4 cut(s)	422, 1516, 2098, 3608
Eco57I	CTGAAG	2 cut(s)	93, 1395
Eco72I	CACGTG	1 cut(s)	2603
Eco91I	GGTNACC	2 cut(s)	1095, 2833
EcoNI	CCTNNNNNAGG	1 cut(s)	1808
EcoO65I	GGTNACC	2 cut(s)	1095, 2833
EcoRI	GAATTC	5 cut(s)	773, 1322, 1834, 2046, 3090
EcoRII	CCWGG	8 cut(s)	603, 1056, 1213, 1327, 1878, 1926, 3024, 3622
EcoRV	GATATC	1 cut(s)	3492
EcoT14I	CCWWGG	2 cut(s)	455, 1156
EcoT22I	ATGCAT	1 cut(s)	2683
ErhI	CCWWGG	2 cut(s)	455, 1156
FalI	AAGNNNNNCTT	4 cut(s)	1175, 1207, 2581, 2613
FaqI	GGGAC	2 cut(s)	1445, 3094
FauNDI	CATATG	3 cut(s)	625, 3073, 3200
FbaI	TGATCA	2 cut(s)	211, 1636
Fnu4HI	GCNGC	7 cut(s)	186, 1033, 1472, 1923, 1950, 2019, 3634
Fsp4HI	GCNGC	7 cut(s)	186, 1033, 1472, 1923, 1950, 2019, 3634
FspBI	CTAG	9 cut(s)	299, 564, 1172, 1367, 1584, 2043, 2405, 2613, 3380
GluI	GCNGC	7 cut(s)	186, 1033, 1472, 1923, 1950, 2019, 3634
GsuI	CTGGAG	2 cut(s)	2381, 3214
HaeIII	GGCC	6 cut(s)	537, 1154, 1200, 1618, 1803, 1890
HapII	CCGG	2 cut(s)	445, 1820
HincII	GTYRAC	4 cut(s)	556, 1594, 2001, 3510
HindII	GTYRAC	4 cut(s)	556, 1594, 2001, 3510
HindIII	AAGCTT	2 cut(s)	372, 2558
HinfI	GANTC	9 cut(s)	469, 1508, 1595, 2729, 2759, 2783, 2849, 2889, 3481
HpaII	CCGG	2 cut(s)	445, 1820
HphI	GGTGA	9 cut(s)	36, 272, 503, 1089, 2513, 2665, 3047, 3437, 3612
Hpy166II	GTNNAC	9 cut(s)	556, 724, 1594, 2001, 2441, 2516, 3445, 3510, 3586
Hpy8I	GTNNAC	9 cut(s)	556, 724, 1594, 2001, 2441, 2516, 3445, 3510, 3586
HpyCH4III	ACNGT	9 cut(s)	274, 721, 1699, 1998, 2182, 2438, 2669, 2803, 3439
HpyCH4IV	ACGT	2 cut(s)	1748, 2602
HpyF10VI	GCNNNNNNNGC	5 cut(s)	970, 1197, 3075, 3240, 3578
HpySE526I	ACGT	2 cut(s)	1748, 2602
Ksp22I	TGATCA	2 cut(s)	211, 1636
LmnI	GCTCC	8 cut(s)	401, 871, 1108, 2362, 2431, 3009, 3158, 3181
Lsp1109I	GCAGC	6 cut(s)	197, 1458, 1909, 1961, 2005, 3645
MabI	ACCWGGT	2 cut(s)	603, 1878
MaeI	CTAG	9 cut(s)	299, 564, 1172, 1367, 1584, 2043, 2405, 2613, 3380
MaeII	ACGT	2 cut(s)	1748, 2602
MaeIII	GTNAC	8 cut(s)	491, 957, 1095, 1848, 1992, 2332, 2833, 3618
MfeI	CAATTG	6 cut(s)	32, 102, 382, 1527, 3250, 3560
MflI	RGATCY	2 cut(s)	816, 1577
MhlI	GDGCHC	5 cut(s)	726, 1057, 1312, 2436, 2518
MlsI	TGGCCA	1 cut(s)	537
MluNI	TGGCCA	1 cut(s)	537
MlyI	GAGTC	2 cut(s)	1589, 2753
MmeI	TCCRAC	7 cut(s)	186, 1260, 1769, 2469, 2488, 2554, 3011
Mox20I	TGGCCA	1 cut(s)	537
Mph1103I	ATGCAT	1 cut(s)	2683
MroXI	GAANNNNTTC	3 cut(s)	468, 1088, 1959
MscI	TGGCCA	1 cut(s)	537
MslI	CAYNNNNRTG	7 cut(s)	194, 392, 518, 2907, 3171, 3429, 3450
Msp20I	TGGCCA	1 cut(s)	537
MspA1I	CMGCKG	2 cut(s)	1454, 3581
MspI	CCGG	2 cut(s)	445, 1820
MspR9I	CCNGG	9 cut(s)	445, 605, 1058, 1215, 1329, 1880, 1928, 3026, 3624
MunI	CAATTG	6 cut(s)	32, 102, 382, 1527, 3250, 3560
Mva1269I	GAATGC	4 cut(s)	659, 2081, 2387, 2627
MvaI	CCWGG	8 cut(s)	605, 1058, 1215, 1329, 1880, 1928, 3026, 3624
MvnI	CGCG	1 cut(s)	1035
MwoI	GCNNNNNNNGC	5 cut(s)	970, 1197, 3075, 3240, 3578
NciI	CCSGG	1 cut(s)	445
NcoI	CCATGG	1 cut(s)	455
NdeI	CATATG	3 cut(s)	625, 3073, 3200
NheI	GCTAGC	1 cut(s)	1583
NlaIV	GGNNCC	3 cut(s)	873, 2206, 3160
NmuCI	GTSAC	4 cut(s)	491, 1095, 2332, 3618
NsiI	ATGCAT	1 cut(s)	2683
NspI	RCATGY	5 cut(s)	895, 1674, 2681, 2685, 3556
PaeI	GCATGC	1 cut(s)	2681
PceI	AGGCCT	1 cut(s)	1200
PctI	GAATGC	4 cut(s)	659, 2081, 2387, 2627
PdmI	GAANNNNTTC	3 cut(s)	468, 1088, 1959
PfeI	GAWTC	7 cut(s)	469, 1508, 2729, 2783, 2849, 2889, 3481
PkrI	GCNGC	7 cut(s)	187, 1034, 1473, 1924, 1951, 2020, 3635
PleI	GAGTC	2 cut(s)	1589, 2753
PmaCI	CACGTG	1 cut(s)	2603
PmlI	CACGTG	1 cut(s)	2603
PpsI	GAGTC	2 cut(s)	1589, 2753
Ppu21I	YACGTR	1 cut(s)	2603
PshBI	ATTAAT	1 cut(s)	2328
PsiI	TTATAA	1 cut(s)	378
Psp6I	CCWGG	8 cut(s)	603, 1056, 1213, 1327, 1878, 1926, 3024, 3622
PspCI	CACGTG	1 cut(s)	2603
PspEI	GGTNACC	2 cut(s)	1095, 2833
PspGI	CCWGG	8 cut(s)	603, 1056, 1213, 1327, 1878, 1926, 3024, 3622
PspN4I	GGNNCC	3 cut(s)	873, 2206, 3160
PspPI	GGNCC	5 cut(s)	422, 1153, 1516, 2098, 3608
PstI	CTGCAG	1 cut(s)	1476
PstNI	CAGNNNCTG	1 cut(s)	220
PsuI	RGATCY	2 cut(s)	816, 1577
PvuII	CAGCTG	2 cut(s)	1454, 3581
RseI	CAYNNNNRTG	7 cut(s)	194, 392, 518, 2907, 3171, 3429, 3450
SatI	GCNGC	7 cut(s)	186, 1033, 1472, 1923, 1950, 2019, 3634
Sau96I	GGNCC	5 cut(s)	422, 1153, 1516, 2098, 3608
ScaI	AGTACT	2 cut(s)	718, 1187
SchI	GAGTC	2 cut(s)	1589, 2753
ScrFI	CCNGG	9 cut(s)	445, 605, 1058, 1215, 1329, 1880, 1928, 3026, 3624
SduI	GDGCHC	5 cut(s)	726, 1057, 1312, 2436, 2518
SexAI	ACCWGGT	2 cut(s)	603, 1878
SfcI	CTRYAG	1 cut(s)	1472
SinI	GGWCC	4 cut(s)	422, 1516, 2098, 3608
SmiMI	CAYNNNNRTG	7 cut(s)	194, 392, 518, 2907, 3171, 3429, 3450
SmlI	CTYRAG	5 cut(s)	1315, 1808, 2218, 2626, 3149
SmoI	CTYRAG	5 cut(s)	1315, 1808, 2218, 2626, 3149
SphI	GCATGC	1 cut(s)	2681
SseBI	AGGCCT	1 cut(s)	1200
SsiI	CCGC	1 cut(s)	1033
SspI	AATATT	1 cut(s)	3054
SspMI	CTAG	9 cut(s)	299, 564, 1172, 1367, 1584, 2043, 2405, 2613, 3380
StuI	AGGCCT	1 cut(s)	1200
StyD4I	CCNGG	9 cut(s)	443, 603, 1056, 1213, 1327, 1878, 1926, 3024, 3622
StyI	CCWWGG	2 cut(s)	455, 1156
TaaI	ACNGT	9 cut(s)	274, 721, 1699, 1998, 2182, 2438, 2669, 2803, 3439
TaiI	ACGT	2 cut(s)	1751, 2605
TaqI	TCGA	5 cut(s)	1424, 1647, 1691, 2211, 3540
TatI	WGTACW	8 cut(s)	8, 415, 580, 716, 1185, 1699, 2169, 2615
TauI	GCSGC	1 cut(s)	1035
TfiI	GAWTC	7 cut(s)	469, 1508, 2729, 2783, 2849, 2889, 3481
TscAI	CASTG	6 cut(s)	733, 1594, 2116, 2187, 2441, 3643
TseFI	GTSAC	4 cut(s)	491, 1095, 2332, 3618
TseI	GCWGC	6 cut(s)	185, 1471, 1922, 1949, 2018, 3633
Tsp45I	GTSAC	4 cut(s)	491, 1095, 2332, 3618
TspGWI	ACGGA	4 cut(s)	481, 496, 2716, 3621
TspRI	CASTG	6 cut(s)	733, 1594, 2116, 2187, 2441, 3643
VneI	GTGCAC	2 cut(s)	722, 2514
VpaK11BI	GGWCC	4 cut(s)	422, 1516, 2098, 3608
VspI	ATTAAT	1 cut(s)	2328
XagI	CCTNNNNNAGG	1 cut(s)	1808
XbaI	TCTAGA	2 cut(s)	298, 2042
XceI	RCATGY	5 cut(s)	895, 1674, 2681, 2685, 3556
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3428
XmnI	GAANNNNTTC	3 cut(s)	468, 1088, 1959
XspI	CTAG	9 cut(s)	299, 564, 1172, 1367, 1584, 2043, 2405, 2613, 3380
ZrmI	AGTACT	2 cut(s)	718, 1187
Zsp2I	ATGCAT	1 cut(s)	2683

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.