RchiOBHm_Chr1g0361071

Vacuolar protein sorting-associated protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0006617
Physical Location & Seq
Forward (+)
53065653 .. 53075680
10028 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58599

Sequence Viewer

Length: 5826 bp
ATGACCAAGAAGCTCACACAATCGGAACCAAAACTCGCCATGGAGCTCGACCTTGACTCGTTCCTCAACTCACACCTCTCCTCCTCCGACTCCGACTCCGACCCCGACCTCACCTCCGTCCCGCGCCGTACGATCGACGAAATCCTCAACGACACCGATTCATCCAGGTCAGCATCCCCCTCCTCTTCCCCGCGTAGTTCGACCTTATCTCAGTTCCTCGCGTCCGATCCCAAACCGCCACAGAGCGACGCCGTTTCGGTCGCCAGCGGCAGAACCGAGGGAGCGGGGAGGTTGAGTCAATTGGAGGAGAAGGCGGTCCGGCCGGGTTTGAATTTGTACAGCCGGGCTAGGTCCGGGGATTTCCCGGACGATTCGGTCGGGAGAGTTTCGAGGCCGTCGCCGTGGCTGTTTGGAGGTGTGAGGACGAATGCGAAGCCCGGGGCCGCGCTCGCGGCGGCTGCGGCGGCTTCGAGGTCGATGCCGACGCCCCACGCCGCGGCGATCAAGTCGAGGAGGAGCGCCGGGAGCGGGAGTTTCCAGAAAGCTGTGGAGGCTGCGGAATTGGGTGAGAGATCGGAGGGAGGGTCCAATTTTAACGAGGATAGCAATGCGGTTGGTTCTGAAACGACGCCATCGAATTCGAATGAAGTAGAGGTGGATTTTGGTGGTGCGTTGGGGAGGAAAGATGAGGTTTTGGAGAGGGAGAGGGAGGCAGAGCAGGTGAGTCAGATTGATGAAGTTAGTGCTGGCAATGCTGGTGAAGAATTGGATGAGAATTCAACTAATTTAGATGCCACTGATGTTAGAGATAATGAATTGGATGAGAATGTTCGGGTTTCGGAGGAAGGTCCACCGGAGGAAGAAGATTTAGATGAAAATAGTCCTTCTTTGAAACATAGAGATGTTGAGGATGAGCCTACTGGTGAGGATGAGAAGCATCTTGGTGGTATTGATTATAATGATGAGGTTGGTGATAATGATAATGATAAAGATGGAGATGATCATTTTGATGATAAGGATGATGATGCATTGGGAACTTCAATCACTCAACTTGTGGAGGAGAGAATCGAGCAACTGGAGAGCAGGAGGATCAGTAAAAAAGCGGAAAAGAAATCAAGAAAGCCACTTGAAATCGCTGAGGAGCTAGAGAAGAAACAAGCTTCAACTGCTTTGGATTGGGAAGAGGGTGCTGCTGCTCAACCTATGAGACTTGAGGGTGTTCGGAGAGGGTCCACCACATTGGGATACTTCAATGTTGATGCTAAAAATACCATTACGCGGACCCTTTCTGCTCCGGCTTTGAGGCGTGATCATGGTACGCCGCAAGTCCTGGGAGTTCACAGTAATTACATTGCAATAGGAATGTCAAGAGGGGTTGTCCTTGTTGTCCCCAGTAAATACTCTCCTTATAATGCTGACAACATGGATGCAAAGCTGATATTTCTTGGATTGCAAGGAGAGCGGTCTTATGCTGCGGTGACTTCTATATCCTTCAATCAGCAAGGGGATCTACTCTTAGCTGGATACGCCGATGGTCACATTACAGTTTGGGATGTTCAGAGGGCATCCGCTGCCAAAGTAATTACCGGAGAACATACAGCTCCAGTTGTACATACTTTTTTTTTGGGGCATGATTCTCAGGTCACACGTAATTTTAAGGCAGTTACTGGGGACAGTAAGGGTCTGGTACTGTTACATTCCTTCTCTGTGGTTCCCCTGCTTAATAGATTCTCCATCAAAACACAGTGCCTTCTAGACGGACAAAAAACAGGCACGACGCTGTCAGCTTCACCACTCCTCATTGATGAATTGAGTGGAAGCTCTTCACTTTCCTCTCAGGGAAATGTGATGGGTTCAGGCAGCAGTATTGGTGGTATGATGGGTGGTGTGGTTGGTGGGGATGCAGGTTGGAAACTCTTCAATGAAGGATCCTCTTTGTTTGAAGAAGGTGTGGTCGTATTTGTCACCCATCAAAATGCTTTGGTGGTAAGACTAACTCCCACTTTGCATGTCTATGCGCAACTTTCTAAACCAGATGGGGTTCGGGAGGGTTCCATGCCTTGTACTGCTTGGAAATGCACAACTCAATTGCATAATTCACCTACTAATAGTGAGAATACACCTACTGAAGCAGTTGAAAGAGTTTCATTACTTGCAATTGCTTGGGATCGGAAAGTTCAGGTAGCAAAGTTGGTCAAATCAGAGCTGAAAGTATATGGTAAATGGTCTCTTGAAAGTGCAGCCATAGGTGTGGCTTGGTTGGATGATCAGATGCTGGTGGTTCTCACGATAACTGGACAACTTTGTTTGTTTGCAAAGGATGGAACTGGAATTCACCAAACAAGTTTTTCTCTAGATGGTTTTAGAGGGGATGATCTCATTTCTTATCATACTCACTTCATCAATGTATTTGGAAACCCTGAAAAAGCTTATAACAATTGCATAGCTGTAAGAGGAGCTTCTGTATATGTACTTGGACCTACTCATCTTATTGTTTCCCGGCTTCTCCCATGGAAGGAACGGATTCAGGTTTTACGGAGAGCTGGTGACTGGATGGGTTCTTTGAACATGGCAATGACAATTTATGATGGCCAAGCTCATGGTGTTGTTGACCTTCCTAGAACTCTGGATGCTGTACAGGAGGTCATAATGCCCTACCTGGTTGAGTTGCTTTTGTCATATGTAGAGGAAGTTTTTTCTTATATTTCAGTAGCATTCTGCAACCAAATTGAAAAAATGGATCAAGTTGATGACTTAAACAGACAAAGTAGTACTGTGCACACTGAAATTAAAGAGCAATACACCCGTGTTGGTGGGGTTGCAGTTGAATTCTGTGTCCATATCAAGAGGACTGACATACTTTTTGATGAGATCTTCTTGAAGTTTGTAGCTGTTCAACAGAGAGACACATTTTTGGAACTATTGGAGCCTTATATATTGAAGGACATGCTTGGTTCTCTTCCGCCTGAGATTATGCAAGCTCTGGTAGAACATTACAGCCACAAAGGGTGGTTACAGCGTGTTGAGCAATGTGTCCTTCACATGGATATTTCTTCATTGGATTTCAATCAGGTTGTTAGGTTATGCCGCGAGCATGGATTATATAGTGCCCTGGTGTATCTTTTCAACAAAGGATTGAATGATTTCAGGTCACCATTAGAGGAGCTGCTGGTGGTCTTGCGAAATAGTCAAAAAGAAGGTGCTATGGCCCTTGGATACAGGATGCTAGTTTATTTGAAGTACTGCTTTTCAGGCCTTGCTTTTCCACCAGGACAAGGAAAAATTCCTCCTTCACGCTTGCCATCTCTCAGAACAGAACTTTTGCAGTTTTTATTGGAGGGTTCTGATGCCCCTAATTCAAGAGCACTCTCAAGCGAATTCCCAGGAGGAGAATATCTCAACCTTTATTATCTCTTAGAACTAGATACTGAAGCTACTTTAGATGTTCTGAGATGTGCTTTCACAGAAGTTGAAATCTCGAAATGGGACTCCTCTTCTCAAAATTCAGCTGATGCAGATACAGAGCTGCAGTATGGGAATAATTCAACGGCTCAAAGTCAGGATTCATTGGTCCAGAATACAATTGATACTCTTATTCACATTATTAGTAAGGACATTCCCCAGAAGGATGGATCTGCTAGCAGTGTTGACTCTGGATCAGTCGTAGAGTGGCCTTCAAAGAAAGATATTGATCATCTATTCGAGTTTATTGCATATTATGTTGCATGTGGAAAAGCTAATGTCTCGAAGACTGTACTTTCTCAGATTTTGGAGTATTTGACATCAGAGAATAACTTTCCAACGTCTGTTTCTGGAGATAGCATAATTTCAAAAAAAAGAGAGAAGCAGGTTCTTGGCCTTCTTGAGGTAGTGCCGGAGACTGATTGGAATTCATCTTCTGTGTTACAGCTATGTGAGAAAGCACAATTTTACCAGGTATGTGGCCTAATTCATACCAGCAGACATCAGTATATTGCTGCCCTGGATTGCTATATGAAAGATGCAGCCGAACCCATTCATGCCTTTGCCTTTATCAACAAAACATTGTTACAGTTGACTGACGATGAATGTGCTGCTTTTCGATCAGCAGTTATTTCTAGAATTCCAGGGCTATTTAACTTAAACAGAGAGGGTGCATTCTTCTTGGTCATGGACCATTTTACCAGTGCAGAAGGTTCTCATATCCTATCTGAACTTCGCTCCCATCCACGAAGCCTTTTCCTGTACTTAAAAACAGTGATTGAGGTTCATTTATCTGGCACCCTCGACTTCTCAAGTTTAAAAACCGACAATCTTATGGGAGTAAAGGATCAAACAAAAGGAGTTGAGGCTTTCTTGGAAAGGGTCTCTAATTTCCCACAGCTTTTGCGTGATAGTCCTATTAATGTGACTGATGATATGATTGAGCTTTATCTGGAGCTGTTATGCCAGTTTGAATGCAAATCAGTTCTCAAATTCCTAGAGACATTTGATAGCTATCGCGTGGAGCACTGTTTATGCCTCTGCCAGAAATATGCAATAATAGATGCTTCATCGTTCTTGTTGGAGAGGGTTGGTGATGTTGGAAGTGCACTTTTACTTACACTTTCCACCCTCAATGACAAATTTATCAAGCTTGAAACTGCTGTTGGAAGTCTAAATTTAAGCGGGGCTTCACGTGGTTCTGCTAGTACAGAATACTTGAGCAATGCTTTGAAAGTGGAGGAGGTGAATGATATAGACAGTATATTGCATGCATGTATAGGATTGTGCCAACGGAACACCCATCGTCTAAATCCTGATGAATCAGAGGCACTCTGGTTCAAATTGCTTGACTCGTTTTGTGAGCCTTTAATGGATTCGTTTAGTGCTGGTACAGTATCTAAAGGAGAAGATCATAATGGAATGGTAACCAATTCATTGGATTCACAAGAGGATGAAATGAATTTCACAATCAAGTGGAGAATCCCAAAGGCTAATAAAGGTGCTGATATTTTAAGAAAATTGTTTTCTCGGTTCATCAAGGAGATTGTTGAGGGCATGATAGGATATGTTCGCCTTCCAACTATCATGTCAAAGCTCCTTTCTGACAATGGTAGCCAGGAATTTGGTGATTTTAAATTCACAATATTGGGGATGCTTAGCACATATGGCTTCGAAAGAAGAATTCTGGATACTGCCATGTCCCTGATAGAAGATGATACATTCTATACTATGAGCATCCTCAAGAAAGGAGCCTCACATGGGTATGCTCCTCGCAACCCAAAATGTTGCATATGCGATTGCCTCCTTGACAAGAACTCTTCCAGCTACATTCGCATTTTCAATTGTGGTCATGCAACACATCTTAAATGCGAAGTTTCAGAGAATGAAACATCAAGTCGTAGCTCGTCCTCTGGATGCCCAGTTTGTATGCCCAAGAAGAAATCTCAGAGGTCTAAAAACAAATCGGTTCTAGCAGAGGAGAGTTTAGTGAAGGTGTTTTCATCAAGAACCCATCATACTCATGGACCAGTTGTTCACCCACATGAAAGCACTTCTGAGAATAGCTATGGGATTCAGCAGATATCACGGTTTGAGATGTTGACCAATTTGCAGAAACATCGTGGATTAGTCGAGATTGAAAACATGCCTCAATTGAGGCTTGCCCCACCAGCTGTGTACCATGAAAAGGTTAAGCACGGACCTGTTCTGTCACCAGGAGAGAGCAGCAGTGATCTTGTGAGAACAGAGAAACAAAGTAAAATCAAGCAACTCAGGGAGGTAAAAGTAAAAGGATCATCTCTTCGGTTTCCCTTGAAAACAAACTTATTTGGTAATGGCAAGGACAAGATCAGCAGGCGATGA

Protein Analysis

1941

Amino Acids

214.23

Weight (kDa)

5.29

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_VPS8 PF23410 423 - 605 1.1e-22 Vps8 beta-propeller
VPS8_N PF23306 424 - 597 1.6e-06 VPS8-like, N-terminal beta propeller repeats
Beta-prop_WDR36-Utp21_1st PF25171 451 - 543 6.9e-06 WDR36/Utp21 first beta-propeller
TPR_Vps8 PF12816 971 - 1154 5.8e-51 Vps8 central TPR
TPR_Vps41 PF23556 1466 - 1534 5.8e-06 Vps41 TPR-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 957, 1410, 2433
AarI CACCTGC 1 cut(s) 709
AasI GACNNNNNNGTC 1 cut(s) 374
Acc16I TGCGCA 1 cut(s) 2019
Acc36I ACCTGC 3 cut(s) 709, 1895, 3838
AccB1I GGYRCC 1 cut(s) 4259
AccBSI CCGCTC 3 cut(s) 284, 528, 1462
AccII CGCG 9 cut(s) 124, 193, 221, 446, 452, 497, 1279, 3090, 4482
AcoI YGGCCR 2 cut(s) 320, 2590
AcuI CTGAAG 2 cut(s) 2148, 3450
AcvI CACGTG 1 cut(s) 4658
AcyI GRCGYC 3 cut(s) 249, 485, 629
AfiI CCNNNNNNNGG 9 cut(s) 496, 528, 1278, 2515, 3043, 3275, 3865, 5223, 5650
AflIII ACRYGT 1 cut(s) 1646
AjuI GAANNNNNNNTTGG 4 cut(s) 3793, 3825, 4611, 4643
AloI GAACNNNNNNTCC 2 cut(s) 5481, 5513
Alw21I GWGCWC 5 cut(s) 48, 2781, 3367, 4491, 4573
Alw26I GTCTC 7 cut(s) 1201, 2232, 2898, 3748, 3872, 4351, 4457
Alw44I GTGCAC 2 cut(s) 2777, 4569
AlwNI CAGNNNCTG 2 cut(s) 1667, 2275
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 9 cut(s) 320, 392, 441, 2590, 3207, 3253, 3671, 3856, 3943
ApaLI GTGCAC 2 cut(s) 2777, 4569
ArsI GACNNNNNNTTYG 2 cut(s) 2846, 2878
AseI ATTAAT 1 cut(s) 4383
Asp700I GAANNNNTTC 5 cut(s) 1822, 1916, 2523, 3143, 4014
AspLEI GCGC 4 cut(s) 126, 448, 521, 2020
AsuC2I CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2500
AsuII TTCGAA 2 cut(s) 641, 5136
AsuNHI GCTAGC 1 cut(s) 3638
AvaI CYCGRG 1 cut(s) 437
BaeGI GKGCMC 3 cut(s) 2781, 3112, 4573
BalI TGGCCA 1 cut(s) 2592
BamHI GGATCC 1 cut(s) 1928
BanI GGYRCC 1 cut(s) 4259
BanII GRGCYC 1 cut(s) 48
BbrPI CACGTG 1 cut(s) 4658
BbsI GAAGAC 1 cut(s) 3755
Bbv12I GWGCWC 5 cut(s) 48, 2781, 3367, 4491, 4573
BbvCI CCTCAGC 1 cut(s) 1137
BceAI ACGGC 5 cut(s) 111, 236, 379, 385, 3564
BcgI CGANNNNNNTGC 8 cut(s) 460, 494, 3692, 3726, 4052, 4086, 5564, 5598
BciVI GTATCC 4 cut(s) 1238, 1517, 3209, 5146
BclI TGATCA 4 cut(s) 1000, 1309, 2266, 3691
BcnI CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2500
BcoDI GTCTC 7 cut(s) 1201, 2232, 2898, 3748, 3872, 4351, 4457
BfmI CTRYAG 1 cut(s) 3527
BfoI RGCGCY 1 cut(s) 522
BfuAI ACCTGC 3 cut(s) 709, 1895, 3838
BfuI GTATCC 4 cut(s) 1238, 1517, 3209, 5146
BglII AGATCT 1 cut(s) 2871
BlpI GCTNAGC 1 cut(s) 5120
BmcAI AGTACT 2 cut(s) 2773, 3242
BmeT110I CYCGRG 1 cut(s) 437
BmrI ACTGGG 3 cut(s) 1386, 1677, 5378
BmtI GCTAGC 1 cut(s) 3642
BmuI ACTGGG 3 cut(s) 1386, 1677, 5378
BpiI GAAGAC 1 cut(s) 3755
BplI GAGNNNNNCTC 2 cut(s) 3381, 3413
BpmI CTGGAG 4 cut(s) 1097, 1587, 3834, 4436
Bpu10I CCTNAGC 1 cut(s) 1137
Bpu1102I GCTNAGC 1 cut(s) 5120
Bpu14I TTCGAA 2 cut(s) 641, 5136
BpuEI CTTGAG 6 cut(s) 1232, 3355, 3884, 4258, 4702, 5189
BpuMI CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2500
BsaAI YACGTR 2 cut(s) 1649, 4658
BsaBI GATNNNNATC 3 cut(s) 3066, 3690, 4476
BsaHI GRCGYC 3 cut(s) 249, 485, 629
BsaI GGTCTC 2 cut(s) 2232, 4351
BsaWI WCCGGW 2 cut(s) 853, 1586
Bsc4I CCNNNNNNNGG 9 cut(s) 496, 528, 1278, 2515, 3043, 3275, 3865, 5223, 5650
Bse3DI GCAATG 6 cut(s) 613, 757, 1350, 2580, 3035, 4693
Bse8I GATNNNNATC 3 cut(s) 3066, 3690, 4476
BseJI GATNNNNATC 3 cut(s) 3066, 3690, 4476
BseLI CCNNNNNNNGG 9 cut(s) 496, 528, 1278, 2515, 3043, 3275, 3865, 5223, 5650
BseMI GCAATG 6 cut(s) 613, 757, 1350, 2580, 3035, 4693
BseSI GKGCMC 3 cut(s) 2781, 3112, 4573
BseX3I CGGCCG 1 cut(s) 320
BsgI GTGCAG 2 cut(s) 2259, 4188
Bsh1236I CGCG 9 cut(s) 124, 193, 221, 446, 452, 497, 1279, 3090, 4482
Bsh1285I CGRYCG 4 cut(s) 135, 261, 323, 378
BshFI GGCC 9 cut(s) 322, 394, 443, 2592, 3209, 3255, 3673, 3858, 3945
BshNI GGYRCC 1 cut(s) 4259
BsiEI CGRYCG 4 cut(s) 135, 261, 323, 378
BsiHKAI GWGCWC 5 cut(s) 48, 2781, 3367, 4491, 4573
BsiHKCI CYCGRG 1 cut(s) 437
BsiWI CGTACG 1 cut(s) 128
BslFI GGGAC 5 cut(s) 104, 1373, 1685, 3500, 5149
BslI CCNNNNNNNGG 9 cut(s) 496, 528, 1278, 2515, 3043, 3275, 3865, 5223, 5650
BsmAI GTCTC 7 cut(s) 1201, 2232, 2898, 3748, 3872, 4351, 4457
BsmFI GGGAC 5 cut(s) 104, 1373, 1685, 3500, 5149
BsmI GAATGC 4 cut(s) 433, 2714, 4136, 4442
BsnI GGCC 9 cut(s) 322, 394, 443, 2592, 3209, 3255, 3673, 3858, 3945
Bso31I GGTCTC 2 cut(s) 2232, 4351
BsoBI CYCGRG 1 cut(s) 437
Bsp119I TTCGAA 2 cut(s) 641, 5136
Bsp1286I GDGCHC 6 cut(s) 48, 2781, 3112, 3367, 4491, 4573
Bsp1407I TGTACA 3 cut(s) 336, 1609, 2635
Bsp1720I GCTNAGC 1 cut(s) 5120
Bsp19I CCATGG 2 cut(s) 39, 2510
BspANI GGCC 9 cut(s) 322, 394, 443, 2592, 3209, 3255, 3673, 3858, 3945
BspFNI CGCG 9 cut(s) 124, 193, 221, 446, 452, 497, 1279, 3090, 4482
BspMAI CTGCAG 1 cut(s) 3531
BspMI ACCTGC 3 cut(s) 709, 1895, 3838
BspOI GCTAGC 1 cut(s) 3642
BspQI GCTCTTC 1 cut(s) 1828
BspT104I TTCGAA 2 cut(s) 641, 5136
BspT107I GGYRCC 1 cut(s) 4259
BspTNI GGTCTC 2 cut(s) 2232, 4351
BsrBI CCGCTC 3 cut(s) 284, 528, 1462
BsrDI GCAATG 6 cut(s) 613, 757, 1350, 2580, 3035, 4693
BsrGI TGTACA 3 cut(s) 336, 1609, 2635
BssNI GRCGYC 3 cut(s) 249, 485, 629
BssT1I CCWWGG 3 cut(s) 39, 2510, 3211
Bst6I CTCTTC 8 cut(s) 190, 1176, 1828, 1922, 2964, 3499, 5287, 5769
BstACI GRCGYC 3 cut(s) 249, 485, 629
BstAPI GCANNNNNTGC 1 cut(s) 1571
BstAUI TGTACA 3 cut(s) 336, 1609, 2635
BstBAI YACGTR 2 cut(s) 1649, 4658
BstBI TTCGAA 2 cut(s) 641, 5136
BstDSI CCRYGG 4 cut(s) 39, 401, 495, 2510
BstEII GGTNACC 2 cut(s) 3150, 4888
BstENI CCTNNNNNAGG 1 cut(s) 3863
BstFNI CGCG 9 cut(s) 124, 193, 221, 446, 452, 497, 1279, 3090, 4482
BstH2I RGCGCY 1 cut(s) 522
BstHHI GCGC 4 cut(s) 126, 448, 521, 2020
BstMAI GTCTC 7 cut(s) 1201, 2232, 2898, 3748, 3872, 4351, 4457
BstMCI CGRYCG 4 cut(s) 135, 261, 323, 378
BstNSI RCATGY 6 cut(s) 2012, 2950, 3729, 4736, 4740, 5611
BstPI GGTNACC 2 cut(s) 3150, 4888
BstSFI CTRYAG 1 cut(s) 3527
BstSLI GKGCMC 3 cut(s) 2781, 3112, 4573
BstUI CGCG 9 cut(s) 124, 193, 221, 446, 452, 497, 1279, 3090, 4482
BstV2I GAAGAC 1 cut(s) 3755
BstX2I RGATCY 4 cut(s) 1507, 1928, 2871, 3632
BstXI CCANNNNNNTGG 7 cut(s) 1240, 2251, 2600, 3629, 3941, 4900, 5087
BstYI RGATCY 4 cut(s) 1507, 1928, 2871, 3632
BstZI CGGCCG 1 cut(s) 320
BsuI GTATCC 4 cut(s) 1238, 1517, 3209, 5146
BsuRI GGCC 9 cut(s) 322, 394, 443, 2592, 3209, 3255, 3673, 3858, 3945
BtgI CCRYGG 4 cut(s) 39, 401, 495, 2510
BtsI GCAGTG 2 cut(s) 3649, 5698
BtsIMutI CAGTG 8 cut(s) 795, 1751, 2781, 3649, 4171, 4242, 4489, 5698
BveI ACCTGC 3 cut(s) 709, 1895, 3838
CaiI CAGNNNCTG 2 cut(s) 1667, 2275
CfoI GCGC 4 cut(s) 126, 448, 521, 2020
Cfr42I CCGCGG 1 cut(s) 498
Cfr9I CCCGGG 1 cut(s) 437
CpoI CGGWCCG 1 cut(s) 316
CseI GACGC 5 cut(s) 210, 257, 493, 637, 1786
CsiI ACCWGGT 2 cut(s) 2658, 3933
CspCI CAANNNNNGTGG 2 cut(s) 4347, 4382
CspI CGGWCCG 1 cut(s) 316
DraI TTTAAA 2 cut(s) 4281, 5098
DrdI GACNNNNNNGTC 1 cut(s) 374
DseDI GACNNNNNNGTC 1 cut(s) 374
EaeI YGGCCR 2 cut(s) 320, 2590
EagI CGGCCG 1 cut(s) 320
Eam1104I CTCTTC 8 cut(s) 190, 1176, 1828, 1922, 2964, 3499, 5287, 5769
EarI CTCTTC 8 cut(s) 190, 1176, 1828, 1922, 2964, 3499, 5287, 5769
EciI GGCGGA 1 cut(s) 2952
Ecl136II GAGCTC 1 cut(s) 46
EclXI CGGCCG 1 cut(s) 320
Eco130I CCWWGG 3 cut(s) 39, 2510, 3211
Eco147I AGGCCT 1 cut(s) 3255
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 2 cut(s) 2232, 4351
Eco32I GATATC 1 cut(s) 5547
Eco52I CGGCCG 1 cut(s) 320
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 2148, 3450
Eco72I CACGTG 1 cut(s) 4658
Eco88I CYCGRG 1 cut(s) 437
Eco91I GGTNACC 2 cut(s) 3150, 4888
EcoICRI GAGCTC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 3863
EcoO65I GGTNACC 2 cut(s) 3150, 4888
EcoRI GAATTC 8 cut(s) 637, 775, 2331, 2828, 3377, 3889, 4101, 5145
EcoRV GATATC 1 cut(s) 5547
EcoT14I CCWWGG 3 cut(s) 39, 2510, 3211
EcoT22I ATGCAT 2 cut(s) 1030, 4738
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 3 cut(s) 39, 2510, 3211
FalI AAGNNNNNCTT 6 cut(s) 2443, 2475, 3230, 3262, 4636, 4668
FaqI GGGAC 5 cut(s) 104, 1373, 1685, 3500, 5149
FauI CCCGC 5 cut(s) 129, 198, 277, 521, 4640
FauNDI CATATG 3 cut(s) 2680, 5128, 5255
FbaI TGATCA 4 cut(s) 1000, 1309, 2266, 3691
FriOI GRGCYC 1 cut(s) 48
FspI TGCGCA 1 cut(s) 2019
GlaI GCGC 4 cut(s) 125, 447, 520, 2019
GsuI CTGGAG 4 cut(s) 1097, 1587, 3834, 4436
HaeII RGCGCY 1 cut(s) 522
HaeIII GGCC 9 cut(s) 322, 394, 443, 2592, 3209, 3255, 3673, 3858, 3945
HgaI GACGC 5 cut(s) 210, 257, 493, 637, 1786
HhaI GCGC 4 cut(s) 126, 448, 521, 2020
Hin1I GRCGYC 3 cut(s) 249, 485, 629
Hin6I GCGC 4 cut(s) 124, 446, 519, 2018
HinP1I GCGC 4 cut(s) 124, 446, 519, 2018
HincII GTYRAC 4 cut(s) 2611, 3649, 4056, 5565
HindII GTYRAC 4 cut(s) 2611, 3649, 4056, 5565
HindIII AAGCTT 3 cut(s) 1158, 2427, 4613
Hpy99I CGWCG 6 cut(s) 140, 251, 400, 487, 631, 1780
HpyCH4IV ACGT 3 cut(s) 1648, 3803, 4657
HpySE526I ACGT 3 cut(s) 1648, 3803, 4657
Hsp92I GRCGYC 3 cut(s) 249, 485, 629
HspAI GCGC 4 cut(s) 124, 446, 519, 2018
Ksp22I TGATCA 4 cut(s) 1000, 1309, 2266, 3691
KspI CCGCGG 1 cut(s) 498
LguI GCTCTTC 1 cut(s) 1828
MabI ACCWGGT 2 cut(s) 2658, 3933
MaeII ACGT 3 cut(s) 1648, 3803, 4657
MbiI CCGCTC 3 cut(s) 284, 528, 1462
MfeI CAATTG 7 cut(s) 299, 2087, 2157, 2437, 3582, 5305, 5615
MflI RGATCY 4 cut(s) 1507, 1928, 2871, 3632
MhlI GDGCHC 6 cut(s) 48, 2781, 3112, 3367, 4491, 4573
MlsI TGGCCA 1 cut(s) 2592
MluNI TGGCCA 1 cut(s) 2592
MlyI GAGTC 8 cut(s) 50, 83, 89, 304, 733, 3482, 3644, 4808
Mox20I TGGCCA 1 cut(s) 2592
Mph1103I ATGCAT 2 cut(s) 1030, 4738
MroXI GAANNNNTTC 5 cut(s) 1822, 1916, 2523, 3143, 4014
MscI TGGCCA 1 cut(s) 2592
Msp20I TGGCCA 1 cut(s) 2592
MspA1I CMGCKG 5 cut(s) 267, 497, 1571, 3509, 5636
MunI CAATTG 7 cut(s) 299, 2087, 2157, 2437, 3582, 5305, 5615
Mva1269I GAATGC 4 cut(s) 433, 2714, 4136, 4442
MvnI CGCG 9 cut(s) 124, 193, 221, 446, 452, 497, 1279, 3090, 4482
NciI CCSGG 8 cut(s) 324, 344, 355, 365, 438, 439, 523, 2500
NcoI CCATGG 2 cut(s) 39, 2510
NdeI CATATG 3 cut(s) 2680, 5128, 5255
NheI GCTAGC 1 cut(s) 3638
NmuCI GTSAC 8 cut(s) 1477, 1535, 1642, 1963, 2546, 3150, 4387, 5673
NsbI TGCGCA 1 cut(s) 2019
NsiI ATGCAT 2 cut(s) 1030, 4738
NspI RCATGY 6 cut(s) 2012, 2950, 3729, 4736, 4740, 5611
NspV TTCGAA 2 cut(s) 641, 5136
PaeI GCATGC 1 cut(s) 4736
PaqCI CACCTGC 1 cut(s) 709
PceI AGGCCT 1 cut(s) 3255
PciSI GCTCTTC 1 cut(s) 1828
PcsI WCGNNNNNNNCGW 1 cut(s) 632
PctI GAATGC 4 cut(s) 433, 2714, 4136, 4442
PdmI GAANNNNTTC 5 cut(s) 1822, 1916, 2523, 3143, 4014
Pfl23II CGTACG 1 cut(s) 128
PflFI GACNNNGTC 1 cut(s) 1780
PfoI TCCNGGA 1 cut(s) 363
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 8 cut(s) 50, 83, 89, 303, 732, 3482, 3644, 4808
PmaCI CACGTG 1 cut(s) 4658
PmlI CACGTG 1 cut(s) 4658
PpsI GAGTC 8 cut(s) 50, 83, 89, 303, 732, 3482, 3644, 4808
Ppu21I YACGTR 2 cut(s) 1649, 4658
PshBI ATTAAT 1 cut(s) 4383
PsiI TTATAA 3 cut(s) 957, 1410, 2433
Psp124BI GAGCTC 1 cut(s) 48
PspCI CACGTG 1 cut(s) 4658
PspEI GGTNACC 2 cut(s) 3150, 4888
PspLI CGTACG 1 cut(s) 128
PsrI GAACNNNNNNTAC 2 cut(s) 5464, 5496
PstI CTGCAG 1 cut(s) 3531
PstNI CAGNNNCTG 2 cut(s) 1667, 2275
PsuI RGATCY 4 cut(s) 1507, 1928, 2871, 3632
PsyI GACNNNGTC 1 cut(s) 1780
PvuI CGATCG 1 cut(s) 135
PvuII CAGCTG 2 cut(s) 3509, 5636
Rsr2I CGGWCCG 1 cut(s) 316
RsrII CGGWCCG 1 cut(s) 316
SacI GAGCTC 1 cut(s) 48
SacII CCGCGG 1 cut(s) 498
SapI GCTCTTC 1 cut(s) 1828
ScaI AGTACT 2 cut(s) 2773, 3242
SchI GAGTC 8 cut(s) 50, 83, 89, 304, 733, 3482, 3644, 4808
SduI GDGCHC 6 cut(s) 48, 2781, 3112, 3367, 4491, 4573
SexAI ACCWGGT 2 cut(s) 2658, 3933
SfcI CTRYAG 1 cut(s) 3527
Sfr303I CCGCGG 1 cut(s) 498
SfuI TTCGAA 2 cut(s) 641, 5136
SgrBI CCGCGG 1 cut(s) 498
SmaI CCCGGG 1 cut(s) 439
SmlI CTYRAG 6 cut(s) 1211, 3370, 3863, 4273, 4681, 5204
SmoI CTYRAG 6 cut(s) 1211, 3370, 3863, 4273, 4681, 5204
SphI GCATGC 1 cut(s) 4736
SseBI AGGCCT 1 cut(s) 3255
SspI AATATT 1 cut(s) 5109
SstI GAGCTC 1 cut(s) 48
StuI AGGCCT 1 cut(s) 3255
StyI CCWWGG 3 cut(s) 39, 2510, 3211
TaiI ACGT 3 cut(s) 1651, 3806, 4660
TaqII GACCGA 2 cut(s) 247, 364
TscAI CASTG 8 cut(s) 802, 1751, 2788, 3649, 4171, 4242, 4496, 5698
TseFI GTSAC 8 cut(s) 1477, 1535, 1642, 1963, 2546, 3150, 4387, 5673
Tsp45I GTSAC 8 cut(s) 1477, 1535, 1642, 1963, 2546, 3150, 4387, 5673
TspGWI ACGGA 6 cut(s) 106, 1773, 2536, 2551, 4771, 5676
TspMI CCCGGG 1 cut(s) 437
TspRI CASTG 8 cut(s) 802, 1751, 2788, 3649, 4171, 4242, 4496, 5698
Tth111I GACNNNGTC 1 cut(s) 1780
VneI GTGCAC 2 cut(s) 2777, 4569
VspI ATTAAT 1 cut(s) 4383
XagI CCTNNNNNAGG 1 cut(s) 3863
XbaI TCTAGA 3 cut(s) 1753, 2353, 4097
XceI RCATGY 6 cut(s) 2012, 2950, 3729, 4736, 4740, 5611
XcmI CCANNNNNNNNNTGG 1 cut(s) 5483
XmaI CCCGGG 1 cut(s) 437
XmnI GAANNNNTTC 5 cut(s) 1822, 1916, 2523, 3143, 4014
ZrmI AGTACT 2 cut(s) 2773, 3242
Zsp2I ATGCAT 2 cut(s) 1030, 4738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.