Rh1AG296700

Vacuolar protein sorting-associated protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0006617
Physical Location & Seq
Forward (+)
52768365 .. 52778467
10103 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG296700.1

Sequence Viewer

Length: 5244 bp
ATGACCAAGAAGCTCACACAATCGGAACCACAACTCGCCATGGAGCTCGACCTTGACTCGTTCCTCAACTCACACCCCTCCTCCTCCTCCGACTCCGACCCCGACCTCAACTCCGTCCCGCGCCGTACGATCGACGAAATCCTCAACGACACCGATTCATCCAGGTCAGCATCCCCCTCCTCTTCCCCGCGTAGTTCGACCTTATCTCAGTTCCTCGCGTCCGATCCCAAACCGCCACAGAGCGACGCCGTTTCGGTCGCCAGCGGCAGAACCGAGGGAGCGGGGAGGTTGAGTCAATTGGAGGAGAAGGCGGTCCGGCCGGGTTTGAATTTGTACAGCCGGGCTAGGTCCGGGGATTTCCCGGACGATTCGGTCGGGAGAGTTTCGAGGCCGTCGCCGTGGCTGTTGGGAGGTGTGAGGACGAATGCGAAGCCCGGAGCCGCGCTCGCGGCGGCTGCGGCGGCTTCGAGGTCGATGCCGACGCCCCACGCCGCGGCGATCAAGTCGAGGAGGAGCGCCGGGAGCGGGAGTTTCCAGAAAGCTGTGGAGGCTGCGGAATTGGGTGAGAGATCGGAGGGAGGGTCCAATTTTAACGAGGATAGCAATGCGGTTGGTTCTGAAACGACGCCGTCGAATTCGAATGAAGTAGAGGTGGATTTTGGTGGTGCGTTGGGGAGGAAAGATGAGTTTTTGGAGAGGGAGAGGGAGGTAGAGCAGGTGAGTCAGGTTGATGAAGTTAGTGCTGGCAATGCTGGTGAAGAATTGGATGAGAATTCAACAAATTTAGATGCCACTGATGTTAGAGATAATGAATTGGATGAGAATGTTCGGGTTTCGGAGGAAGGTGCACCGGAGGAAGAAGATTTAGATGAAAATAGTCCTTCTTTGAAACATAGAGATGTTGAGGATGAGCAGCATCTGGGTGGTATTGATGATAATGATGAGGTTGGTGATAATGATAATGATAAATATGGAGATGATCATTTTCATGATAAGGATGATGATGCATTGGGAACTTCAATCACTCAACTTGTGGAGGAGAGAATCGAGCAACTGGAGAGCAGGAGGATCAGTAAAAAAGCGGAAAAGAAATCAAGAAAGCCACTTGAAATCGCTGAGGAGCTAGAGAAGAAACAAGCTTCAACTGCTTTGGATTGGGAAGAGGGTGCTGCTGCTCAACCTATGAGACTTGAGGGTGTTCGGAGAGGGTCCACCACATTGGGATACTTCAATGTTGATGCTAAAAATACCATTACGCGGACCCTTTCTGCTCCGGCTTTGAGGCGTGATCATGGTACGCCGCAAGTCCTGGGAGTTCACAGTAATTACATTGCAATAGGAATGTCAAGAGGGGTTGTCCTTGTTGTCCCCAGTAAATACTCTCCTTATAATGCTGACAACATGGATGCAAAGCTGATATTTCTTGGATTGCAAGGAGAGCGGTCTTATGCTGCGGTAACTTCTATATCCTTCAATCAGCAAGGGGATCTACTCTTAGCTGGATACGCCGATGGTCACATTACAGTTTGGGATGTTCAGAGGGCATCCGCTGCCAAAGTAATTACCGGAGAACATACAGCTCCAGTTGTACATACTTTTTTTTTGGGGCATGATTCTCAGGTCACACGTAATTTTAAGGCAGTTACTGGGGATAGTAAGGGTCTGGTACTGTTACATTCCTTCTCTGTGGTTCCGCTGCTTAATAGATTCTCCATCAAAACACAGTGCCTTCTAGATGGACAAAAAACAGGCACGACGCTGTCAGCTTCACCACTCCTCATTGATGAATTGAGTGGAAGCTCTTCACTTTCCTCTCAGGGAAATGCGATGGGTTCAGGCAGCAGTATTGGTGGTATGATGGGTGGTGTGGTTGGTGGGGATGCAGGTTGGAAACTCTTCAATGAAGGATCCTCTTTGTTTGAAGAAGGTGTGGTCGTATTTGTCACCCATCAAAATGCTTTGGTGGTAAGACTGACTCCCACTTTGCATGTCTATGCACAACTTTCTAAACCGGATGGGGTTCGGGAGGGTTCCATGCCTTGTACTGCTTGGAAATGCACAACTCAGTTGCATAATTCACCTACTAATAGTGAGAATACGCCTACTGAAGCAGTTGAAAGAGTTTCATTACTTGCAATTGCTTGGGATCGGAAAGTTCAGGTAGCAAAGTTGGTCAAATCAGAGCTGAAAGTATATGGTAAATGGTCTCTTGAAAGTGCAGCCATAGGTGTGGCTTGGTTGGATGATCAGATGCTGGTGGTTCTCACGATAACTGGACAACTTTGTTTGTTTGCAAAGGATGGAACTGGAATTCACCAAACAAGTTTTTCTCTAGATGGTTTTAGAGGGGATGATCTCATTTCTTATCATACTCACTTCATCAATGTATTTGGAAACCCTGAAAAAGCTTATAACAATTGCATAGCTGTAAGAGGAGCTTCTGTATATGTACTTGGACCTACTCATCTTATTGTTTCCCGGCTTCTCCCATGGAAGGAACGGATTCAGGTTTTACGGAGAGCTGGTGACTGGATGGGTTCTTTGAACATGGCAATGACAATTTATGATGGCCAAGCTCATGGTGTTGTTGACCTTCCTAGAACTCTGGATGCTGTACAGGAGGTCATAATGCCCTACCTGGTTGAGTTGCTTTTGTCATATGTAGAGGAAGTTTTTTCTTATATTTCAGTAGCATTCTGCAACCAAATTGAAAAAATGGATCAAGTTGATGACTTAAACAGACAAAGTAGTACTGTGCACACTGAAATTAAAGAGCAATACACCCGTGTTGGTGGGGTTGCAGTTGAATTCTGTGTCCATATCAAGAGGACTGACATACTTTTTGATGAGATCTTCTTGAAGTTTGTAGCTGTTCAACAGAGAGACACATTTTTGGAACTATTGGAGCCTTATATATTGAAGGACATGCTTGGTTCTCTTCCGCCTGAGATTATGCAAGCTCTGGTAGAACATTACAGCCACAAAGGGTGGTTACAGCGTGTTGAGCAATGTGTCCTTCACATGGATATTTCTTCATTGGATTTCAATCAGGTTGTTAGGTTATGCCGCGAGCACGGATTATATAGTGCCCTGGTGTATCTTTTCAACAAAGGATTGAATGATTTCAGGTCACCATTAGAGGAGCTGTTGGTGGTCTTGCGAAATAGTCAAAAAGAAGTTGCTATGGCCCTTGGATACAGGATGCTAGTTTATTTGAAGTACTGCTTTTCAGGCCTTGCTTTTCCACCAGGACAAGGAAAAATTCCTCCTTCACGCTTGCCATCTCTCAGAACAGAACTTTTGCAGTTTTTATTGGAGGGTTCTGATGCCCCTAATTCAAGAGCACTCTCAAGCGAATTCCCAGGAGGAGAATATCTCAACCTTTATTATCTCTTAGAACTAGATACTGAAGCTACTTTAGATGTTCTGAGATGTGCTTTCACAGAAGTTGAAATCTCGAAATGGGACTCCTCTTCTCAAAATTCAGCTGATGCAGATACAGAGCTGCAGTATGGGAATAATTCAACAGCTCAAAGTCAGGATTCATTGGTCCAGAATACAATTGATACTCTTATTCACATTATTAGTAAGGACATTCCCCAGAAGGATGGATCTGCTAGCACTGTTGACTCTGGATCAGTCGTAGAGTGGCCTTCAAAGAAAGATATTGATCATCTATTCGAGTTTATTGCATATTATGTTGCATGTGGAAAAGCTAATGTCTCGAAGACTGTACTTTCTCAGATTTTGGAGTATTTGACATCAGAGAATAACTTTCCAACGTCTGTTTCTGGAGATAGCATAATTTCAAAAAGAAGAGAGAAGCAGGTTCTTGGCCTTCTTGAGGTAGTGCCGGAGACTGATTGGAATTCATCTTCTGTGTTACAGTTATGTGAGAAAGCACAATTTTACCAGGTATGTGGCCTAATTCATACCAGCAGACATCAGTATATTGCTGCCCTGGATTGCTATATGAAAGATGCAGCCGAACCCATTCATGCCTTTGCCTTTATCAACAAAACATTGTTACAGTTGACTGACGATGAATGTGCTGCTTTTCGATCAGCAGTTATTTCTAGAATTCCAGGGCTATTTAACTTAAACAGAGAGGGTGCATTCTTCTTGGTCATGGACCATTTTACCAGTGCAGAAGGTTCTCATATCCTATCTGAACTTCGCTCCCATCCACGAAGCCTTTTCCTGTACTTAAAAACAGTGATTGAGGTTCATTTATCTGGCACCCTCGACTTCTCAAGTTTAAAAACCGACAATCTTATGGGAGTAAAGGATCAAACAAAAGGAGTTGAGGCTTTCTTGGAAAGGGTCTCTAATTTCCCACAGCTTTTGCGTGATAGTCCTATTAATGTGACTGATGATATGATTGAACTTTATCTGGAGCTGTTATGCCAGTTTGAATGCAAATCAGTTCTCAAATTCCTAGAGACATTTGATAGCTATCGCGTGGAGCACTGTTTACGCCTCTGCCAGAAATATGCAATAATAGATGCTTCATCATTCTTGTTGGAGAGGGTTGGTGATGTTGGAAGTGCACTTTTACTTACACTTTCCACCCTCAATGACAAATTTATCAAGCTTGAAACTGCTGTTGGAAGTCTAAATTTAAGTGGGGCTTCACGTGGTTCTGCTAGTACAGAATGCTTGAGCAATGCTTTGAAAGTGGAGGAGGTGAATGATATAGACAGTATATTGCATGCATGTATAGGATTGTGCCAACGGAACACCCATCGTCTAAATCCTGATGAATCAGAGGCACTCTGGTTCAAATTGCTTGACTCGTTTTGTGAGCCTTTAATGGATTCGTTTAGTGCTGGTACAGTATCTAAAGGAGAAGATCATAATAGAATGGTAACCAATTCATTGGATTCACAAGAGGATGAAATGAATTTCACAATCAAGTGGAGAATCCCAAAGGCTCATAAAGGTGCTGATATTTTAAGAAAATTGTTTTCTCGGTTCATCAAGGAGATTGTTGAGGGCATGATAGGATATGTTCGCCTTCCAACTATCATGTCAAAGCTCCTTTCTGACAATGGTAGCCAGGAATTTGGTGATTTTAAATTCACAATATTGGGGATGCTTAGCACATATGGCTTTGAAAGAAGAATTCTGGTACAGCAGCTCTATTTTAATCTCAGTTATGCTAGTTATGTACATGATGTATTCTTGTTCAAGGGAATTATTGTGTCAAAGTCATTCTTGTGGAGTGTACTTACAAGGCAATGA

Protein Analysis

1747

Amino Acids

193.03

Weight (kDa)

5.06

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_VPS8 PF23410 416 - 600 8.4e-23 Vps8 beta-propeller
VPS8_N PF23306 417 - 590 1.5e-06 VPS8-like, N-terminal beta propeller repeats
Beta-prop_WDR36-Utp21_1st PF25171 444 - 536 6.1e-06 WDR36/Utp21 first beta-propeller
TPR_Vps8 PF12816 964 - 1147 5.8e-51 Vps8 central TPR
TPR_Vps41 PF23556 1459 - 1527 1.1e-06 Vps41 TPR-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1389, 2412
AarI CACCTGC 1 cut(s) 706
AasI GACNNNNNNGTC 1 cut(s) 371
Acc36I ACCTGC 3 cut(s) 706, 1874, 3817
AccB1I GGYRCC 1 cut(s) 4238
AccBSI CCGCTC 3 cut(s) 281, 525, 1441
AccII CGCG 9 cut(s) 121, 190, 218, 443, 449, 494, 1258, 3069, 4461
AcoI YGGCCR 2 cut(s) 317, 2569
AcuI CTGAAG 2 cut(s) 2127, 3429
AcvI CACGTG 1 cut(s) 4637
AcyI GRCGYC 3 cut(s) 246, 482, 626
AfiI CCNNNNNNNGG 7 cut(s) 493, 525, 1257, 2494, 3022, 3254, 3844
AflIII ACRYGT 1 cut(s) 1625
AjuI GAANNNNNNNTTGG 4 cut(s) 3772, 3804, 4590, 4622
Alw21I GWGCWC 7 cut(s) 48, 850, 2760, 3075, 3346, 4470, 4552
Alw26I GTCTC 7 cut(s) 1180, 2211, 2877, 3727, 3851, 4330, 4436
Alw44I GTGCAC 3 cut(s) 846, 2756, 4548
AlwNI CAGNNNCTG 3 cut(s) 919, 1646, 2254
AoxI GGCC 8 cut(s) 317, 389, 2569, 3186, 3232, 3650, 3835, 3922
ApaLI GTGCAC 3 cut(s) 846, 2756, 4548
ArsI GACNNNNNNTTYG 4 cut(s) 1966, 1998, 2825, 2857
AseI ATTAAT 1 cut(s) 4362
Asp700I GAANNNNTTC 5 cut(s) 1801, 1895, 2502, 3122, 3993
AspLEI GCGC 3 cut(s) 123, 445, 518
AspS9I GGNCC 9 cut(s) 313, 348, 582, 1209, 1260, 2456, 3187, 3550, 4132
AsuC2I CCSGG 7 cut(s) 321, 341, 352, 362, 435, 520, 2479
AsuII TTCGAA 1 cut(s) 638
AsuNHI GCTAGC 1 cut(s) 3617
AvaII GGWCC 8 cut(s) 313, 348, 582, 1209, 1260, 2456, 3550, 4132
BaeGI GKGCMC 4 cut(s) 850, 2760, 3091, 4552
BalI TGGCCA 1 cut(s) 2571
BamHI GGATCC 1 cut(s) 1907
BanI GGYRCC 1 cut(s) 4238
BanII GRGCYC 1 cut(s) 48
BbrPI CACGTG 1 cut(s) 4637
BbsI GAAGAC 1 cut(s) 3734
Bbv12I GWGCWC 7 cut(s) 48, 850, 2760, 3075, 3346, 4470, 4552
BbvCI CCTCAGC 1 cut(s) 1116
BceAI ACGGC 5 cut(s) 108, 233, 376, 382, 613
BcgI CGANNNNNNTGC 6 cut(s) 457, 491, 3671, 3705, 4031, 4065
BciVI GTATCC 3 cut(s) 1217, 1496, 3188
BclI TGATCA 4 cut(s) 979, 1288, 2245, 3670
BcnI CCSGG 7 cut(s) 321, 341, 352, 362, 435, 520, 2479
BcoDI GTCTC 7 cut(s) 1180, 2211, 2877, 3727, 3851, 4330, 4436
BfmI CTRYAG 1 cut(s) 3506
BfoI RGCGCY 1 cut(s) 519
BfuAI ACCTGC 3 cut(s) 706, 1874, 3817
BfuI GTATCC 3 cut(s) 1217, 1496, 3188
BglII AGATCT 1 cut(s) 2850
BlpI GCTNAGC 1 cut(s) 5099
BmcAI AGTACT 2 cut(s) 2752, 3221
Bme18I GGWCC 8 cut(s) 313, 348, 582, 1209, 1260, 2456, 3550, 4132
BmgT120I GGNCC 9 cut(s) 313, 348, 582, 1209, 1260, 2456, 3187, 3550, 4132
BmrI ACTGGG 2 cut(s) 1365, 1656
BmtI GCTAGC 1 cut(s) 3621
BmuI ACTGGG 2 cut(s) 1365, 1656
BpiI GAAGAC 1 cut(s) 3734
BplI GAGNNNNNCTC 4 cut(s) 429, 461, 3360, 3392
BpmI CTGGAG 4 cut(s) 1076, 1566, 3813, 4415
Bpu10I CCTNAGC 1 cut(s) 1116
Bpu1102I GCTNAGC 1 cut(s) 5099
Bpu14I TTCGAA 1 cut(s) 638
BpuEI CTTGAG 5 cut(s) 1211, 3334, 3863, 4237, 4681
BpuMI CCSGG 7 cut(s) 321, 341, 352, 362, 435, 520, 2479
BsaAI YACGTR 2 cut(s) 1628, 4637
BsaBI GATNNNNATC 3 cut(s) 3045, 3669, 4455
BsaHI GRCGYC 3 cut(s) 246, 482, 626
BsaI GGTCTC 2 cut(s) 2211, 4330
BsaWI WCCGGW 3 cut(s) 850, 1565, 2011
Bsc4I CCNNNNNNNGG 7 cut(s) 493, 525, 1257, 2494, 3022, 3254, 3844
Bse1I ACTGG 9 cut(s) 1059, 1371, 1583, 1651, 2278, 2311, 2534, 4143, 4408
Bse3DI GCAATG 6 cut(s) 610, 754, 1329, 2559, 3014, 4672
Bse8I GATNNNNATC 3 cut(s) 3045, 3669, 4455
BseJI GATNNNNATC 3 cut(s) 3045, 3669, 4455
BseLI CCNNNNNNNGG 7 cut(s) 493, 525, 1257, 2494, 3022, 3254, 3844
BseMI GCAATG 6 cut(s) 610, 754, 1329, 2559, 3014, 4672
BseNI ACTGG 9 cut(s) 1059, 1371, 1583, 1651, 2278, 2311, 2534, 4143, 4408
BseSI GKGCMC 4 cut(s) 850, 2760, 3091, 4552
BseX3I CGGCCG 1 cut(s) 317
BsgI GTGCAG 2 cut(s) 2238, 4167
Bsh1236I CGCG 9 cut(s) 121, 190, 218, 443, 449, 494, 1258, 3069, 4461
Bsh1285I CGRYCG 4 cut(s) 132, 258, 320, 375
BshFI GGCC 8 cut(s) 319, 391, 2571, 3188, 3234, 3652, 3837, 3924
BshNI GGYRCC 1 cut(s) 4238
BsiEI CGRYCG 4 cut(s) 132, 258, 320, 375
BsiHKAI GWGCWC 7 cut(s) 48, 850, 2760, 3075, 3346, 4470, 4552
BsiWI CGTACG 1 cut(s) 125
BslFI GGGAC 3 cut(s) 101, 1352, 3479
BslI CCNNNNNNNGG 7 cut(s) 493, 525, 1257, 2494, 3022, 3254, 3844
BsmAI GTCTC 7 cut(s) 1180, 2211, 2877, 3727, 3851, 4330, 4436
BsmFI GGGAC 3 cut(s) 101, 1352, 3479
BsmI GAATGC 5 cut(s) 430, 2693, 4115, 4421, 4661
BsnI GGCC 8 cut(s) 319, 391, 2571, 3188, 3234, 3652, 3837, 3924
Bso31I GGTCTC 2 cut(s) 2211, 4330
Bsp119I TTCGAA 1 cut(s) 638
Bsp1286I GDGCHC 8 cut(s) 48, 850, 2760, 3075, 3091, 3346, 4470, 4552
Bsp1407I TGTACA 4 cut(s) 333, 1588, 2614, 5170
Bsp1720I GCTNAGC 1 cut(s) 5099
Bsp19I CCATGG 2 cut(s) 39, 2489
BspANI GGCC 8 cut(s) 319, 391, 2571, 3188, 3234, 3652, 3837, 3924
BspFNI CGCG 9 cut(s) 121, 190, 218, 443, 449, 494, 1258, 3069, 4461
BspHI TCATGA 1 cut(s) 988
BspMAI CTGCAG 1 cut(s) 3510
BspMI ACCTGC 3 cut(s) 706, 1874, 3817
BspOI GCTAGC 1 cut(s) 3621
BspQI GCTCTTC 1 cut(s) 1807
BspT104I TTCGAA 1 cut(s) 638
BspT107I GGYRCC 1 cut(s) 4238
BspTNI GGTCTC 2 cut(s) 2211, 4330
BsrBI CCGCTC 3 cut(s) 281, 525, 1441
BsrDI GCAATG 6 cut(s) 610, 754, 1329, 2559, 3014, 4672
BsrGI TGTACA 4 cut(s) 333, 1588, 2614, 5170
BsrI ACTGG 9 cut(s) 1059, 1371, 1583, 1651, 2278, 2311, 2534, 4143, 4408
BssNI GRCGYC 3 cut(s) 246, 482, 626
BssT1I CCWWGG 3 cut(s) 39, 2489, 3190
Bst6I CTCTTC 7 cut(s) 187, 1155, 1807, 1901, 2943, 3478, 3811
BstACI GRCGYC 3 cut(s) 246, 482, 626
BstAPI GCANNNNNTGC 1 cut(s) 1550
BstAUI TGTACA 4 cut(s) 333, 1588, 2614, 5170
BstBAI YACGTR 2 cut(s) 1628, 4637
BstBI TTCGAA 1 cut(s) 638
BstC8I GCNNGC 8 cut(s) 262, 447, 745, 2958, 3071, 3278, 3619, 4713
BstDSI CCRYGG 4 cut(s) 39, 398, 492, 2489
BstEII GGTNACC 2 cut(s) 3129, 4867
BstENI CCTNNNNNAGG 1 cut(s) 3842
BstFNI CGCG 9 cut(s) 121, 190, 218, 443, 449, 494, 1258, 3069, 4461
BstH2I RGCGCY 1 cut(s) 519
BstHHI GCGC 3 cut(s) 123, 445, 518
BstMAI GTCTC 7 cut(s) 1180, 2211, 2877, 3727, 3851, 4330, 4436
BstMCI CGRYCG 4 cut(s) 132, 258, 320, 375
BstNSI RCATGY 5 cut(s) 1991, 2929, 3708, 4715, 4719
BstPI GGTNACC 2 cut(s) 3129, 4867
BstSFI CTRYAG 1 cut(s) 3506
BstSLI GKGCMC 4 cut(s) 850, 2760, 3091, 4552
BstUI CGCG 9 cut(s) 121, 190, 218, 443, 449, 494, 1258, 3069, 4461
BstV2I GAAGAC 1 cut(s) 3734
BstX2I RGATCY 4 cut(s) 1486, 1907, 2850, 3611
BstXI CCANNNNNNTGG 7 cut(s) 1219, 2230, 2579, 3608, 3920, 4879, 5066
BstYI RGATCY 4 cut(s) 1486, 1907, 2850, 3611
BstZI CGGCCG 1 cut(s) 317
BsuI GTATCC 3 cut(s) 1217, 1496, 3188
BsuRI GGCC 8 cut(s) 319, 391, 2571, 3188, 3234, 3652, 3837, 3924
BtgI CCRYGG 4 cut(s) 39, 398, 492, 2489
BtgZI GCGATG 1 cut(s) 1841
BtsIMutI CAGTG 7 cut(s) 792, 1730, 2760, 3621, 4150, 4221, 4468
BveI ACCTGC 3 cut(s) 706, 1874, 3817
Cac8I GCNNGC 8 cut(s) 262, 447, 745, 2958, 3071, 3278, 3619, 4713
CaiI CAGNNNCTG 3 cut(s) 919, 1646, 2254
CciI TCATGA 1 cut(s) 988
CfoI GCGC 3 cut(s) 123, 445, 518
Cfr13I GGNCC 9 cut(s) 313, 348, 582, 1209, 1260, 2456, 3187, 3550, 4132
Cfr42I CCGCGG 1 cut(s) 495
CpoI CGGWCCG 1 cut(s) 313
CseI GACGC 5 cut(s) 207, 254, 490, 634, 1765
CsiI ACCWGGT 2 cut(s) 2637, 3912
CspCI CAANNNNNGTGG 2 cut(s) 4326, 4361
CspI CGGWCCG 1 cut(s) 313
DraI TTTAAA 2 cut(s) 4260, 5077
DrdI GACNNNNNNGTC 1 cut(s) 371
DseDI GACNNNNNNGTC 1 cut(s) 371
EaeI YGGCCR 2 cut(s) 317, 2569
EagI CGGCCG 1 cut(s) 317
Eam1104I CTCTTC 7 cut(s) 187, 1155, 1807, 1901, 2943, 3478, 3811
EarI CTCTTC 7 cut(s) 187, 1155, 1807, 1901, 2943, 3478, 3811
EciI GGCGGA 1 cut(s) 2931
Ecl136II GAGCTC 1 cut(s) 46
EclXI CGGCCG 1 cut(s) 317
Eco130I CCWWGG 3 cut(s) 39, 2489, 3190
Eco147I AGGCCT 1 cut(s) 3234
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 2 cut(s) 2211, 4330
Eco47I GGWCC 8 cut(s) 313, 348, 582, 1209, 1260, 2456, 3550, 4132
Eco52I CGGCCG 1 cut(s) 317
Eco53kI GAGCTC 1 cut(s) 46
Eco57I CTGAAG 2 cut(s) 2127, 3429
Eco72I CACGTG 1 cut(s) 4637
Eco91I GGTNACC 2 cut(s) 3129, 4867
EcoICRI GAGCTC 1 cut(s) 46
EcoNI CCTNNNNNAGG 1 cut(s) 3842
EcoO65I GGTNACC 2 cut(s) 3129, 4867
EcoRI GAATTC 8 cut(s) 634, 772, 2310, 2807, 3356, 3868, 4080, 5124
EcoT14I CCWWGG 3 cut(s) 39, 2489, 3190
EcoT22I ATGCAT 2 cut(s) 1009, 4717
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 3 cut(s) 39, 2489, 3190
FalI AAGNNNNNCTT 8 cut(s) 2422, 2454, 3209, 3241, 4615, 4647, 5201, 5233
FaqI GGGAC 3 cut(s) 101, 1352, 3479
FauI CCCGC 4 cut(s) 126, 195, 274, 518
FauNDI CATATG 2 cut(s) 2659, 5107
FbaI TGATCA 4 cut(s) 979, 1288, 2245, 3670
FriOI GRGCYC 1 cut(s) 48
GlaI GCGC 3 cut(s) 122, 444, 517
GsuI CTGGAG 4 cut(s) 1076, 1566, 3813, 4415
HaeII RGCGCY 1 cut(s) 519
HaeIII GGCC 8 cut(s) 319, 391, 2571, 3188, 3234, 3652, 3837, 3924
HgaI GACGC 5 cut(s) 207, 254, 490, 634, 1765
HhaI GCGC 3 cut(s) 123, 445, 518
Hin1I GRCGYC 3 cut(s) 246, 482, 626
Hin6I GCGC 3 cut(s) 121, 443, 516
HinP1I GCGC 3 cut(s) 121, 443, 516
HincII GTYRAC 3 cut(s) 2590, 3628, 4035
HindII GTYRAC 3 cut(s) 2590, 3628, 4035
HindIII AAGCTT 3 cut(s) 1137, 2406, 4592
Hpy99I CGWCG 7 cut(s) 137, 248, 397, 484, 628, 634, 1759
HpyCH4IV ACGT 3 cut(s) 1627, 3782, 4636
HpySE526I ACGT 3 cut(s) 1627, 3782, 4636
Hsp92I GRCGYC 3 cut(s) 246, 482, 626
HspAI GCGC 3 cut(s) 121, 443, 516
Ksp22I TGATCA 4 cut(s) 979, 1288, 2245, 3670
KspI CCGCGG 1 cut(s) 495
LguI GCTCTTC 1 cut(s) 1807
MabI ACCWGGT 2 cut(s) 2637, 3912
MaeII ACGT 3 cut(s) 1627, 3782, 4636
MbiI CCGCTC 3 cut(s) 281, 525, 1441
MfeI CAATTG 4 cut(s) 296, 2136, 2416, 3561
MflI RGATCY 4 cut(s) 1486, 1907, 2850, 3611
MhlI GDGCHC 8 cut(s) 48, 850, 2760, 3075, 3091, 3346, 4470, 4552
MlsI TGGCCA 1 cut(s) 2571
MluNI TGGCCA 1 cut(s) 2571
MlyI GAGTC 8 cut(s) 50, 86, 301, 730, 1969, 3461, 3623, 4787
MmeI TCCRAC 9 cut(s) 114, 120, 1868, 2220, 3803, 4503, 4522, 4588, 5045
Mox20I TGGCCA 1 cut(s) 2571
Mph1103I ATGCAT 2 cut(s) 1009, 4717
MroXI GAANNNNTTC 5 cut(s) 1801, 1895, 2502, 3122, 3993
MscI TGGCCA 1 cut(s) 2571
MslI CAYNNNNRTG 9 cut(s) 897, 921, 987, 1953, 1992, 2228, 2552, 4941, 5218
Msp20I TGGCCA 1 cut(s) 2571
MspA1I CMGCKG 5 cut(s) 264, 494, 1550, 1696, 3488
MunI CAATTG 4 cut(s) 296, 2136, 2416, 3561
Mva1269I GAATGC 5 cut(s) 430, 2693, 4115, 4421, 4661
MvnI CGCG 9 cut(s) 121, 190, 218, 443, 449, 494, 1258, 3069, 4461
NciI CCSGG 7 cut(s) 321, 341, 352, 362, 435, 520, 2479
NcoI CCATGG 2 cut(s) 39, 2489
NdeI CATATG 2 cut(s) 2659, 5107
NheI GCTAGC 1 cut(s) 3617
NmuCI GTSAC 6 cut(s) 1514, 1621, 1942, 2525, 3129, 4366
NsiI ATGCAT 2 cut(s) 1009, 4717
NspI RCATGY 5 cut(s) 1991, 2929, 3708, 4715, 4719
NspV TTCGAA 1 cut(s) 638
PaeI GCATGC 1 cut(s) 4715
PagI TCATGA 1 cut(s) 988
PaqCI CACCTGC 1 cut(s) 706
PceI AGGCCT 1 cut(s) 3234
PciSI GCTCTTC 1 cut(s) 1807
PcsI WCGNNNNNNNCGW 1 cut(s) 629
PctI GAATGC 5 cut(s) 430, 2693, 4115, 4421, 4661
PdmI GAANNNNTTC 5 cut(s) 1801, 1895, 2502, 3122, 3993
Pfl23II CGTACG 1 cut(s) 125
PflFI GACNNNGTC 2 cut(s) 628, 1759
PfoI TCCNGGA 1 cut(s) 360
Ple19I CGATCG 1 cut(s) 132
PleI GAGTC 8 cut(s) 50, 86, 300, 729, 1969, 3461, 3623, 4787
PmaCI CACGTG 1 cut(s) 4637
PmlI CACGTG 1 cut(s) 4637
PpsI GAGTC 8 cut(s) 50, 86, 300, 729, 1969, 3461, 3623, 4787
Ppu21I YACGTR 2 cut(s) 1628, 4637
PshBI ATTAAT 1 cut(s) 4362
PsiI TTATAA 2 cut(s) 1389, 2412
Psp124BI GAGCTC 1 cut(s) 48
PspCI CACGTG 1 cut(s) 4637
PspEI GGTNACC 2 cut(s) 3129, 4867
PspLI CGTACG 1 cut(s) 125
PspPI GGNCC 9 cut(s) 313, 348, 582, 1209, 1260, 2456, 3187, 3550, 4132
PstI CTGCAG 1 cut(s) 3510
PstNI CAGNNNCTG 3 cut(s) 919, 1646, 2254
PsuI RGATCY 4 cut(s) 1486, 1907, 2850, 3611
PsyI GACNNNGTC 2 cut(s) 628, 1759
PvuI CGATCG 1 cut(s) 132
PvuII CAGCTG 1 cut(s) 3488
RseI CAYNNNNRTG 9 cut(s) 897, 921, 987, 1953, 1992, 2228, 2552, 4941, 5218
Rsr2I CGGWCCG 1 cut(s) 313
RsrII CGGWCCG 1 cut(s) 313
SacI GAGCTC 1 cut(s) 48
SacII CCGCGG 1 cut(s) 495
SapI GCTCTTC 1 cut(s) 1807
Sau96I GGNCC 9 cut(s) 313, 348, 582, 1209, 1260, 2456, 3187, 3550, 4132
ScaI AGTACT 2 cut(s) 2752, 3221
SchI GAGTC 8 cut(s) 50, 86, 301, 730, 1969, 3461, 3623, 4787
SduI GDGCHC 8 cut(s) 48, 850, 2760, 3075, 3091, 3346, 4470, 4552
SexAI ACCWGGT 2 cut(s) 2637, 3912
SfcI CTRYAG 1 cut(s) 3506
Sfr303I CCGCGG 1 cut(s) 495
SfuI TTCGAA 1 cut(s) 638
SgrBI CCGCGG 1 cut(s) 495
SinI GGWCC 8 cut(s) 313, 348, 582, 1209, 1260, 2456, 3550, 4132
SmiMI CAYNNNNRTG 9 cut(s) 897, 921, 987, 1953, 1992, 2228, 2552, 4941, 5218
SmlI CTYRAG 5 cut(s) 1190, 3349, 3842, 4252, 4660
SmoI CTYRAG 5 cut(s) 1190, 3349, 3842, 4252, 4660
SphI GCATGC 1 cut(s) 4715
SseBI AGGCCT 1 cut(s) 3234
SspI AATATT 1 cut(s) 5088
SstI GAGCTC 1 cut(s) 48
StuI AGGCCT 1 cut(s) 3234
StyI CCWWGG 3 cut(s) 39, 2489, 3190
TaiI ACGT 3 cut(s) 1630, 3785, 4639
TaqII GACCGA 2 cut(s) 244, 361
TscAI CASTG 7 cut(s) 799, 1730, 2767, 3628, 4150, 4221, 4475
TseFI GTSAC 6 cut(s) 1514, 1621, 1942, 2525, 3129, 4366
Tsp45I GTSAC 6 cut(s) 1514, 1621, 1942, 2525, 3129, 4366
TspGWI ACGGA 5 cut(s) 103, 2515, 2530, 3090, 4750
TspRI CASTG 7 cut(s) 799, 1730, 2767, 3628, 4150, 4221, 4475
Tth111I GACNNNGTC 2 cut(s) 628, 1759
VneI GTGCAC 3 cut(s) 846, 2756, 4548
VpaK11BI GGWCC 8 cut(s) 313, 348, 582, 1209, 1260, 2456, 3550, 4132
VspI ATTAAT 1 cut(s) 4362
XagI CCTNNNNNAGG 1 cut(s) 3842
XbaI TCTAGA 3 cut(s) 1732, 2332, 4076
XceI RCATGY 5 cut(s) 1991, 2929, 3708, 4715, 4719
XmnI GAANNNNTTC 5 cut(s) 1801, 1895, 2502, 3122, 3993
ZrmI AGTACT 2 cut(s) 2752, 3221
Zsp2I ATGCAT 2 cut(s) 1009, 4717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.