FvH4_7g23410

protein At2g39795, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008278
Physical Location & Seq
Forward (+)
18267540 .. 18269001
1462 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g23410.t1

Sequence Viewer

Length: 753 bp
ATGGCTTTCACTTCCATCCTTCGCCGATCGGCCTCATCTCTGGCCCCACTAGCCTCCCGCCTCGTCCGCGGCCAACGGAACTACCACGCCGCCGTGTTCACCGCCATAAACCACTCCAGCTTCGCCGCCCGGAAGCCCTCTTTGAGCCCTCTGGCCCCCAACCCTCGCTACTACTCCAAGGCCGACGACAGCCTTCTCCGAGTCATCGACGCCGAGATTCAGTGCGCCGAGGAGACCGATGAGCAGGAGATTGAAGAGGTTCCGAGTGGTTTCCCTTTTAAGGTGGAAGATGTGCCCGGATTTCAGACTGTGAAACTAACCAGAACGTATCAAGATGAAGAGATTGAGGTGGAAGTTCACATGCCTGATCTTGTTACTGGTGAAGGTGAGGATGATGATGTGGATGATGATGGTAACGAGCATGCCAATCAATCGAGCATTCCATTGGTCGTGACCGTCTCCAAAGGTAATGGGGCTCCGGTTCTTGAGTTTAGCTGCAATGCTTATCCTGATGAGATTGCAATTGAGAGCTTGCTAGTCAAGAATCCTGACACTGCTGAGGATCAAATCGCCTATGAAGGGCCGGACTTCAATGATCTGGATGAGAATCTCCAGAAGTCATTCCACAAGTACTTGGAGATCAGGGGAATTAAGCCCAGCACAACCAACTTTTTGCATGAGTACATGATCAACAAAGACAGCAGGGAATACAAGAATTGGTTGAAGAAACTGAAACAGTTTGTTCAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
MAM33

Protein Analysis

251

Amino Acids

28.14

Weight (kDa)

4.65

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAM33 PF02330 70 - 248 5.2e-51 Mitochondrial glycoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 69
AciI CCGC 6 cut(s) 58, 67, 69, 90, 102, 126
AclWI GGATC 1 cut(s) 570
AcoI YGGCCR 1 cut(s) 70
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 2 cut(s) 632, 683
AfiI CCNNNNNNNGG 2 cut(s) 280, 579
AgsI TTSAA 4 cut(s) 254, 592, 724, 746
AluBI AGCT 3 cut(s) 120, 495, 531
AluI AGCT 3 cut(s) 120, 495, 531
Alw26I GTCTC 2 cut(s) 227, 463
AlwI GGATC 1 cut(s) 570
AoxI GGCC 6 cut(s) 30, 42, 70, 153, 180, 581
ApeKI GCWGC 1 cut(s) 495
Asp700I GAANNNNTTC 2 cut(s) 258, 620
AspLEI GCGC 1 cut(s) 227
AspS9I GGNCC 3 cut(s) 43, 154, 581
AsuC2I CCSGG 2 cut(s) 130, 297
AsuHPI GGTGA 3 cut(s) 91, 392, 398
BaeGI GKGCMC 1 cut(s) 297
BanII GRGCYC 2 cut(s) 149, 478
BbvCI CCTCAGC 1 cut(s) 558
BbvI GCAGC 1 cut(s) 482
BccI CCATC 2 cut(s) 23, 404
BceAI ACGGC 1 cut(s) 77
BclI TGATCA 1 cut(s) 687
BcnI CCSGG 2 cut(s) 130, 297
BcoDI GTCTC 2 cut(s) 227, 463
BfaI CTAG 2 cut(s) 50, 536
BisI GCNGC 4 cut(s) 70, 90, 126, 496
BlsI GCNGC 4 cut(s) 71, 91, 127, 497
BmcAI AGTACT 1 cut(s) 632
Bme1390I CCNGG 2 cut(s) 130, 297
BmgT120I GGNCC 3 cut(s) 43, 154, 581
BmiI GGNNCC 4 cut(s) 45, 156, 261, 477
BmrFI CCNGG 2 cut(s) 130, 297
BpmI CTGGAG 2 cut(s) 100, 596
Bpu10I CCTNAGC 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 506
BpuMI CCSGG 2 cut(s) 130, 297
BsaBI GATNNNNATC 1 cut(s) 606
BsaHI GRCGYC 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 3 cut(s) 67, 177, 228
BsaWI WCCGGW 1 cut(s) 478
Bsc4I CCNNNNNNNGG 2 cut(s) 280, 579
Bse1I ACTGG 1 cut(s) 382
Bse3DI GCAATG 1 cut(s) 505
Bse8I GATNNNNATC 1 cut(s) 606
BseDI CCNNGG 3 cut(s) 67, 177, 228
BseGI GGATG 4 cut(s) 15, 397, 409, 607
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 2 cut(s) 280, 579
BseMI GCAATG 1 cut(s) 505
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 1 cut(s) 382
BseRI GAGGAG 1 cut(s) 245
BseSI GKGCMC 1 cut(s) 297
BseXI GCAGC 1 cut(s) 482
BseYI CCCAGC 1 cut(s) 656
Bsh1236I CGCG 1 cut(s) 69
Bsh1285I CGRYCG 1 cut(s) 29
BshFI GGCC 6 cut(s) 32, 44, 72, 155, 182, 583
BsiEI CGRYCG 1 cut(s) 29
BsiSI CCGG 4 cut(s) 130, 297, 479, 584
BslI CCNNNNNNNGG 2 cut(s) 280, 579
BsmAI GTCTC 2 cut(s) 227, 463
BsmBI CGTCTC 1 cut(s) 463
BsmI GAATGC 1 cut(s) 438
BsnI GGCC 6 cut(s) 32, 44, 72, 155, 182, 583
Bso31I GGTCTC 1 cut(s) 227
Bsp1286I GDGCHC 3 cut(s) 149, 297, 478
Bsp143I GATC 6 cut(s) 26, 367, 562, 595, 639, 687
BspACI CCGC 6 cut(s) 58, 67, 69, 90, 102, 126
BspANI GGCC 6 cut(s) 32, 44, 72, 155, 182, 583
BspCNI CTCAG 1 cut(s) 550
BspFNI CGCG 1 cut(s) 69
BspLI GGNNCC 4 cut(s) 45, 156, 261, 477
BspPI GGATC 1 cut(s) 570
BspTNI GGTCTC 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 505
BsrI ACTGG 1 cut(s) 382
BssECI CCNNGG 3 cut(s) 67, 177, 228
BssMI GATC 6 cut(s) 26, 367, 562, 595, 639, 687
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 177
Bst4CI ACNGT 3 cut(s) 310, 457, 738
Bst6I CTCTTC 2 cut(s) 249, 333
BstACI GRCGYC 1 cut(s) 210
BstC8I GCNNGC 2 cut(s) 423, 533
BstDEI CTNAG 1 cut(s) 558
BstDSI CCRYGG 1 cut(s) 67
BstF5I GGATG 4 cut(s) 15, 397, 409, 607
BstFNI CGCG 1 cut(s) 69
BstHHI GCGC 1 cut(s) 227
BstKTI GATC 6 cut(s) 29, 370, 565, 598, 642, 690
BstMAI GTCTC 2 cut(s) 227, 463
BstMBI GATC 6 cut(s) 26, 367, 562, 595, 639, 687
BstMCI CGRYCG 1 cut(s) 29
BstMWI GCNNNNNNNGC 2 cut(s) 50, 66
BstNSI RCATGY 2 cut(s) 364, 425
BstSCI CCNGG 2 cut(s) 128, 295
BstSLI GKGCMC 1 cut(s) 297
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 1 cut(s) 482
BsuRI GGCC 6 cut(s) 32, 44, 72, 155, 182, 583
BtgI CCRYGG 1 cut(s) 67
BtsCI GGATG 4 cut(s) 15, 397, 409, 607
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 2 cut(s) 227, 552
Cac8I GCNNGC 2 cut(s) 423, 533
CfoI GCGC 1 cut(s) 227
Cfr13I GGNCC 3 cut(s) 43, 154, 581
Cfr42I CCGCGG 1 cut(s) 70
CseI GACGC 1 cut(s) 218
Csp6I GTAC 2 cut(s) 631, 682
CviAII CATG 4 cut(s) 361, 422, 677, 685
CviQI GTAC 2 cut(s) 631, 682
DdeI CTNAG 1 cut(s) 558
DpnI GATC 6 cut(s) 28, 369, 564, 597, 641, 689
DpnII GATC 6 cut(s) 26, 367, 562, 595, 639, 687
EaeI YGGCCR 1 cut(s) 70
Eam1104I CTCTTC 2 cut(s) 249, 333
EarI CTCTTC 2 cut(s) 249, 333
Eco130I CCWWGG 1 cut(s) 177
Eco24I GRGCYC 2 cut(s) 149, 478
Eco31I GGTCTC 1 cut(s) 227
EcoT14I CCWWGG 1 cut(s) 177
EcoT38I GRGCYC 2 cut(s) 149, 478
ErhI CCWWGG 1 cut(s) 177
Esp3I CGTCTC 1 cut(s) 463
FaeI CATG 4 cut(s) 364, 425, 680, 688
FaiI YATR 6 cut(s) 107, 362, 423, 576, 678, 686
FatI CATG 4 cut(s) 360, 421, 676, 684
FauI CCCGC 1 cut(s) 65
FbaI TGATCA 1 cut(s) 687
Fnu4HI GCNGC 4 cut(s) 70, 90, 126, 496
FokI GGATG 4 cut(s) 2, 404, 416, 614
FriOI GRGCYC 2 cut(s) 149, 478
Fsp4HI GCNGC 4 cut(s) 70, 90, 126, 496
FspBI CTAG 2 cut(s) 50, 536
GlaI GCGC 1 cut(s) 226
GluI GCNGC 4 cut(s) 70, 90, 126, 496
GsaI CCCAGC 1 cut(s) 660
GsuI CTGGAG 2 cut(s) 100, 596
HaeIII GGCC 6 cut(s) 32, 44, 72, 155, 182, 583
HapII CCGG 4 cut(s) 130, 297, 479, 584
HgaI GACGC 1 cut(s) 218
HhaI GCGC 1 cut(s) 227
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 4 cut(s) 364, 425, 680, 688
Hin6I GCGC 1 cut(s) 225
HinP1I GCGC 1 cut(s) 225
HinfI GANTC 4 cut(s) 201, 217, 544, 607
HpaII CCGG 4 cut(s) 130, 297, 479, 584
HphI GGTGA 3 cut(s) 91, 392, 398
Hpy166II GTNNAC 2 cut(s) 99, 358
Hpy188I TCNGA 3 cut(s) 200, 264, 306
Hpy188III TCNNGA 8 cut(s) 332, 451, 485, 509, 541, 548, 599, 613
Hpy8I GTNNAC 2 cut(s) 99, 358
Hpy99I CGWCG 2 cut(s) 188, 212
HpyAV CCTTC 4 cut(s) 29, 203, 377, 572
HpyCH4III ACNGT 3 cut(s) 310, 457, 738
HpyCH4IV ACGT 1 cut(s) 326
HpyCH4V TGCA 3 cut(s) 498, 521, 676
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 66
HpyF3I CTNAG 1 cut(s) 558
HpySE526I ACGT 1 cut(s) 326
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 4 cut(s) 364, 425, 680, 688
HspAI GCGC 1 cut(s) 225
Ksp22I TGATCA 1 cut(s) 687
KspI CCGCGG 1 cut(s) 70
Kzo9I GATC 6 cut(s) 26, 367, 562, 595, 639, 687
LmnI GCTCC 1 cut(s) 481
Lsp1109I GCAGC 1 cut(s) 482
MaeI CTAG 2 cut(s) 50, 536
MaeII ACGT 1 cut(s) 326
MaeIII GTNAC 3 cut(s) 373, 413, 451
MalI GATC 6 cut(s) 28, 369, 564, 597, 641, 689
MboI GATC 6 cut(s) 26, 367, 562, 595, 639, 687
MboII GAAGA 4 cut(s) 266, 299, 350, 736
MfeI CAATTG 1 cut(s) 522
MhlI GDGCHC 3 cut(s) 149, 297, 478
MluCI AATT 3 cut(s) 522, 648, 715
MlyI GAGTC 1 cut(s) 210
MroXI GAANNNNTTC 2 cut(s) 258, 620
MseI TTAA 2 cut(s) 279, 651
MspA1I CMGCKG 1 cut(s) 69
MspI CCGG 4 cut(s) 130, 297, 479, 584
MspR9I CCNGG 2 cut(s) 130, 297
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 1 cut(s) 438
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 2 cut(s) 50, 66
NciI CCSGG 2 cut(s) 130, 297
NdeII GATC 6 cut(s) 26, 367, 562, 595, 639, 687
NlaIII CATG 4 cut(s) 364, 425, 680, 688
NlaIV GGNNCC 4 cut(s) 45, 156, 261, 477
NmeAIII GCCGAG 2 cut(s) 238, 253
NmuCI GTSAC 1 cut(s) 451
NspI RCATGY 2 cut(s) 364, 425
PaeI GCATGC 1 cut(s) 425
PctI GAATGC 1 cut(s) 438
PdmI GAANNNNTTC 2 cut(s) 258, 620
PfeI GAWTC 3 cut(s) 217, 544, 607
PkrI GCNGC 4 cut(s) 71, 91, 127, 497
Ple19I CGATCG 1 cut(s) 29
PleI GAGTC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 209
PspFI CCCAGC 1 cut(s) 656
PspN4I GGNNCC 4 cut(s) 45, 156, 261, 477
PspPI GGNCC 3 cut(s) 43, 154, 581
PvuI CGATCG 1 cut(s) 29
RsaI GTAC 2 cut(s) 632, 683
RsaNI GTAC 2 cut(s) 631, 682
SacII CCGCGG 1 cut(s) 70
SaqAI TTAA 2 cut(s) 279, 651
SatI GCNGC 4 cut(s) 70, 90, 126, 496
Sau3AI GATC 6 cut(s) 26, 367, 562, 595, 639, 687
Sau96I GGNCC 3 cut(s) 43, 154, 581
ScaI AGTACT 1 cut(s) 632
SchI GAGTC 1 cut(s) 210
ScrFI CCNGG 2 cut(s) 130, 297
SduI GDGCHC 3 cut(s) 149, 297, 478
SetI ASST 9 cut(s) 122, 261, 285, 329, 351, 388, 469, 497, 533
Sfr303I CCGCGG 1 cut(s) 70
SgrBI CCGCGG 1 cut(s) 70
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
SphI GCATGC 1 cut(s) 425
Sse9I AATT 3 cut(s) 522, 648, 715
SsiI CCGC 6 cut(s) 58, 67, 69, 90, 102, 126
SspMI CTAG 2 cut(s) 50, 536
StyD4I CCNGG 2 cut(s) 128, 295
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 3 cut(s) 310, 457, 738
TaiI ACGT 1 cut(s) 329
TaqI TCGA 2 cut(s) 207, 434
TaqII GACCGA 1 cut(s) 251
TasI AATT 3 cut(s) 522, 648, 715
TatI WGTACW 2 cut(s) 630, 681
TauI GCSGC 3 cut(s) 72, 92, 128
TfiI GAWTC 3 cut(s) 217, 544, 607
Tru1I TTAA 2 cut(s) 279, 651
Tru9I TTAA 2 cut(s) 279, 651
TscAI CASTG 2 cut(s) 227, 559
TseFI GTSAC 1 cut(s) 451
TseI GCWGC 1 cut(s) 495
Tsp45I GTSAC 1 cut(s) 451
TspDTI ATGAA 2 cut(s) 351, 591
TspGWI ACGGA 1 cut(s) 91
TspRI CASTG 2 cut(s) 227, 559
XceI RCATGY 2 cut(s) 364, 425
XmnI GAANNNNTTC 2 cut(s) 258, 620
XspI CTAG 2 cut(s) 50, 536
ZrmI AGTACT 1 cut(s) 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.