RchiOBHm_Chr1g0368531

protein At2g39795, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0008278
Physical Location & Seq
Forward (+)
58622920 .. 58624598
1679 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59287

Sequence Viewer

Length: 756 bp
ATGGCTTTCACTTCGATCCTTCGCAGATCGGCCTCATCTCTGGCCCCACTAGCCACCCGGCTCGTCCGCGGCCAACGAAACTACCACGCCGCCGTCGTCACCGCCATAAACCACTCCAGCTTCGCCGGCCGGAAGCCCTCCTCTCTGAGCCCTCTGGTTCCAAACCCTCGCTACTATTCCAAGGCCGACGACAGCCTTCTCCGAGTCATCGACGCCGAGATTCAGTGCGCCGAGGAGACCGATGATCAGGAGATTGAAGAGGTTCCGAGTGGTTTCCCTTTTAAGATAGAAGATGAGCCTGGATTTCAGACTGTGAAACTGAGGAGAACTTATCAAGATGAAGAGATTCAAGTGGAAGTTCACATGCCTGATCTAGTTACTGGCGAAGGTGAGGATGATGATGTGGATGATGATGGTAACGAACACGCCAATCAATCGAGCATCCCATTGGTCGTGAGCGTCTCCAAGGGTAGTGGCGCCCCTATTCTAGAGTTTAGCTGCAATGCTTTCCCTGATGAGATTGCAATTGAAAGCTTGATTGTTAAGAATCCTGATATTTCTGAGGATCAAATCGCCTACGAGGGGCCTGACTTCCATGATTTGGATGAGAATCTCCAGAAGTCCTTCCACAAGTACTTGGAGATTAGGGGAATCAAGCCCAGCACAACCAATTTCTTGCATGAGTACATGATCAATAAAGACAGCAGGGAATATAAGAACTGGTTGAAGAAACTTAAGCAGTTCGTTCAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
MAM33

Protein Analysis

251

Amino Acids

28.32

Weight (kDa)

4.72

Isoelectric Point (pI)

53.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAM33 PF02330 71 - 249 3.7e-49 Mitochondrial glycoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 476
AccB7I CCANNNNNTGG 1 cut(s) 601
AccII CGCG 1 cut(s) 69
AciI CCGC 4 cut(s) 67, 69, 90, 102
AclWI GGATC 2 cut(s) 10, 573
AcoI YGGCCR 2 cut(s) 70, 127
AcyI GRCGYC 2 cut(s) 213, 477
AfaI GTAC 2 cut(s) 635, 686
AfiI CCNNNNNNNGG 2 cut(s) 582, 601
AflII CTTAAG 1 cut(s) 734
AgsI TTSAA 5 cut(s) 257, 350, 530, 727, 749
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 3 cut(s) 120, 498, 534
AluI AGCT 3 cut(s) 120, 498, 534
Alw26I GTCTC 2 cut(s) 230, 466
AlwI GGATC 2 cut(s) 10, 573
AoxI GGCC 6 cut(s) 30, 42, 70, 127, 183, 584
ApeKI GCWGC 1 cut(s) 498
Asp700I GAANNNNTTC 3 cut(s) 261, 345, 623
AspLEI GCGC 2 cut(s) 230, 479
AspS9I GGNCC 2 cut(s) 43, 584
AsuC2I CCSGG 1 cut(s) 58
AsuHPI GGTGA 2 cut(s) 91, 401
BanI GGYRCC 1 cut(s) 476
BanII GRGCYC 1 cut(s) 152
BbvI GCAGC 1 cut(s) 485
BccI CCATC 1 cut(s) 407
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 300
BclI TGATCA 2 cut(s) 244, 690
BcnI CCSGG 1 cut(s) 58
BcoDI GTCTC 2 cut(s) 230, 466
BfaI CTAG 3 cut(s) 50, 374, 488
BfoI RGCGCY 1 cut(s) 480
BfrI CTTAAG 1 cut(s) 734
BisI GCNGC 3 cut(s) 70, 90, 499
BlsI GCNGC 3 cut(s) 71, 91, 500
BmcAI AGTACT 1 cut(s) 635
Bme1390I CCNGG 2 cut(s) 58, 300
BmgT120I GGNCC 2 cut(s) 43, 584
BmiI GGNNCC 5 cut(s) 45, 159, 264, 478, 585
BmrFI CCNGG 2 cut(s) 58, 300
BmsI GCATC 1 cut(s) 450
BpmI CTGGAG 2 cut(s) 100, 599
BpuMI CCSGG 1 cut(s) 58
BsaBI GATNNNNATC 1 cut(s) 609
BsaHI GRCGYC 2 cut(s) 213, 477
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 4 cut(s) 67, 180, 231, 465
Bsc4I CCNNNNNNNGG 2 cut(s) 582, 601
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 2 cut(s) 385, 725
Bse3DI GCAATG 1 cut(s) 508
Bse8I GATNNNNATC 1 cut(s) 609
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 4 cut(s) 67, 180, 231, 465
BseGI GGATG 4 cut(s) 400, 412, 441, 610
BseJI GATNNNNATC 1 cut(s) 609
BseLI CCNNNNNNNGG 2 cut(s) 582, 601
BseMI GCAATG 1 cut(s) 508
BseMII CTCAG 3 cut(s) 137, 311, 552
BseNI ACTGG 2 cut(s) 385, 725
BseRI GAGGAG 3 cut(s) 130, 248, 337
BseX3I CGGCCG 1 cut(s) 127
BseXI GCAGC 1 cut(s) 485
BseYI CCCAGC 1 cut(s) 659
Bsh1236I CGCG 1 cut(s) 69
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 6 cut(s) 32, 44, 72, 129, 185, 586
BshNI GGYRCC 1 cut(s) 476
BsiEI CGRYCG 1 cut(s) 130
BsiSI CCGG 3 cut(s) 58, 126, 130
BslI CCNNNNNNNGG 2 cut(s) 582, 601
BsmAI GTCTC 2 cut(s) 230, 466
BsmBI CGTCTC 1 cut(s) 466
BsnI GGCC 6 cut(s) 32, 44, 72, 129, 185, 586
Bso31I GGTCTC 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 6 cut(s) 15, 26, 244, 370, 565, 690
BspACI CCGC 4 cut(s) 67, 69, 90, 102
BspANI GGCC 6 cut(s) 32, 44, 72, 129, 185, 586
BspCNI CTCAG 3 cut(s) 138, 312, 553
BspFNI CGCG 1 cut(s) 69
BspLI GGNNCC 5 cut(s) 45, 159, 264, 478, 585
BspPI GGATC 2 cut(s) 10, 573
BspT107I GGYRCC 1 cut(s) 476
BspTI CTTAAG 1 cut(s) 734
BspTNI GGTCTC 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 508
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 2 cut(s) 385, 725
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 4 cut(s) 67, 180, 231, 465
BssMI GATC 6 cut(s) 15, 26, 244, 370, 565, 690
BssNI GRCGYC 2 cut(s) 213, 477
BssT1I CCWWGG 2 cut(s) 180, 465
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 313
Bst6I CTCTTC 2 cut(s) 252, 336
BstACI GRCGYC 2 cut(s) 213, 477
BstAFI CTTAAG 1 cut(s) 734
BstC8I GCNNGC 1 cut(s) 127
BstDEI CTNAG 3 cut(s) 146, 320, 561
BstDSI CCRYGG 1 cut(s) 67
BstF5I GGATG 4 cut(s) 400, 412, 441, 610
BstFNI CGCG 1 cut(s) 69
BstH2I RGCGCY 1 cut(s) 480
BstHHI GCGC 2 cut(s) 230, 479
BstKTI GATC 6 cut(s) 18, 29, 247, 373, 568, 693
BstMAI GTCTC 2 cut(s) 230, 466
BstMBI GATC 6 cut(s) 15, 26, 244, 370, 565, 690
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 2 cut(s) 50, 126
BstNI CCWGG 1 cut(s) 300
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 2 cut(s) 56, 298
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 1 cut(s) 485
BstZI CGGCCG 1 cut(s) 127
BsuRI GGCC 6 cut(s) 32, 44, 72, 129, 185, 586
BtgI CCRYGG 1 cut(s) 67
BtsCI GGATG 4 cut(s) 400, 412, 441, 610
BtsIMutI CAGTG 1 cut(s) 230
Cac8I GCNNGC 1 cut(s) 127
CfoI GCGC 2 cut(s) 230, 479
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 43, 584
Cfr42I CCGCGG 1 cut(s) 70
CseI GACGC 2 cut(s) 221, 448
Csp6I GTAC 2 cut(s) 634, 685
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviAII CATG 4 cut(s) 364, 596, 680, 688
CviQI GTAC 2 cut(s) 634, 685
DdeI CTNAG 3 cut(s) 146, 320, 561
DinI GGCGCC 1 cut(s) 478
DpnI GATC 6 cut(s) 17, 28, 246, 372, 567, 692
DpnII GATC 6 cut(s) 15, 26, 244, 370, 565, 690
EaeI YGGCCR 2 cut(s) 70, 127
EagI CGGCCG 1 cut(s) 127
Eam1104I CTCTTC 2 cut(s) 252, 336
EarI CTCTTC 2 cut(s) 252, 336
EclXI CGGCCG 1 cut(s) 127
Eco130I CCWWGG 2 cut(s) 180, 465
Eco24I GRGCYC 1 cut(s) 152
Eco31I GGTCTC 1 cut(s) 230
Eco52I CGGCCG 1 cut(s) 127
EcoO109I RGGNCCY 1 cut(s) 584
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 2 cut(s) 180, 465
EcoT38I GRGCYC 1 cut(s) 152
EgeI GGCGCC 1 cut(s) 478
EheI GGCGCC 1 cut(s) 478
ErhI CCWWGG 2 cut(s) 180, 465
Esp3I CGTCTC 1 cut(s) 466
FaeI CATG 4 cut(s) 367, 599, 683, 691
FaiI YATR 6 cut(s) 107, 365, 597, 681, 689, 714
FatI CATG 4 cut(s) 363, 595, 679, 687
FbaI TGATCA 2 cut(s) 244, 690
Fnu4HI GCNGC 3 cut(s) 70, 90, 499
FokI GGATG 4 cut(s) 407, 419, 428, 617
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 3 cut(s) 70, 90, 499
FspBI CTAG 3 cut(s) 50, 374, 488
GlaI GCGC 2 cut(s) 229, 478
GluI GCNGC 3 cut(s) 70, 90, 499
GsaI CCCAGC 1 cut(s) 663
GsuI CTGGAG 2 cut(s) 100, 599
HaeII RGCGCY 1 cut(s) 480
HaeIII GGCC 6 cut(s) 32, 44, 72, 129, 185, 586
HapII CCGG 3 cut(s) 58, 126, 130
HgaI GACGC 2 cut(s) 221, 448
HhaI GCGC 2 cut(s) 230, 479
Hin1I GRCGYC 2 cut(s) 213, 477
Hin1II CATG 4 cut(s) 367, 599, 683, 691
Hin6I GCGC 2 cut(s) 228, 477
HinP1I GCGC 2 cut(s) 228, 477
HindIII AAGCTT 1 cut(s) 532
HinfI GANTC 6 cut(s) 204, 220, 346, 547, 610, 651
HpaII CCGG 3 cut(s) 58, 126, 130
HphI GGTGA 2 cut(s) 91, 401
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 5 cut(s) 147, 203, 267, 309, 562
Hpy188III TCNNGA 7 cut(s) 248, 335, 454, 488, 551, 616, 749
Hpy8I GTNNAC 1 cut(s) 361
Hpy99I CGWCG 3 cut(s) 98, 191, 215
HpyAV CCTTC 4 cut(s) 29, 206, 380, 634
HpyCH4III ACNGT 1 cut(s) 313
HpyCH4V TGCA 3 cut(s) 501, 524, 679
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 126
HpyF3I CTNAG 3 cut(s) 146, 320, 561
Hsp92I GRCGYC 2 cut(s) 213, 477
Hsp92II CATG 4 cut(s) 367, 599, 683, 691
HspAI GCGC 2 cut(s) 228, 477
KasI GGCGCC 1 cut(s) 476
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Ksp22I TGATCA 2 cut(s) 244, 690
KspI CCGCGG 1 cut(s) 70
Kzo9I GATC 6 cut(s) 15, 26, 244, 370, 565, 690
Lsp1109I GCAGC 1 cut(s) 485
LweI GCATC 1 cut(s) 450
MaeI CTAG 3 cut(s) 50, 374, 488
MaeIII GTNAC 3 cut(s) 97, 376, 416
MalI GATC 6 cut(s) 17, 28, 246, 372, 567, 692
MboI GATC 6 cut(s) 15, 26, 244, 370, 565, 690
MboII GAAGA 4 cut(s) 269, 302, 353, 739
MfeI CAATTG 1 cut(s) 525
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 2 cut(s) 525, 670
Mly113I GGCGCC 1 cut(s) 477
MlyI GAGTC 1 cut(s) 213
MroNI GCCGGC 1 cut(s) 125
MroXI GAANNNNTTC 3 cut(s) 261, 345, 623
MseI TTAA 4 cut(s) 282, 543, 735, 754
MspA1I CMGCKG 1 cut(s) 69
MspCI CTTAAG 1 cut(s) 734
MspI CCGG 3 cut(s) 58, 126, 130
MspR9I CCNGG 2 cut(s) 58, 300
MunI CAATTG 1 cut(s) 525
MvaI CCWGG 1 cut(s) 300
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 2 cut(s) 50, 126
NaeI GCCGGC 1 cut(s) 127
NarI GGCGCC 1 cut(s) 477
NciI CCSGG 1 cut(s) 58
NdeII GATC 6 cut(s) 15, 26, 244, 370, 565, 690
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 4 cut(s) 367, 599, 683, 691
NlaIV GGNNCC 5 cut(s) 45, 159, 264, 478, 585
NmeAIII GCCGAG 2 cut(s) 241, 256
NmuCI GTSAC 1 cut(s) 97
NspI RCATGY 1 cut(s) 367
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PdiI GCCGGC 1 cut(s) 127
PdmI GAANNNNTTC 3 cut(s) 261, 345, 623
PfeI GAWTC 5 cut(s) 220, 346, 547, 610, 651
PflMI CCANNNNNTGG 1 cut(s) 601
PkrI GCNGC 3 cut(s) 71, 91, 500
PleI GAGTC 1 cut(s) 212
PluTI GGCGCC 1 cut(s) 480
PpsI GAGTC 1 cut(s) 212
Psp6I CCWGG 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 659
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 5 cut(s) 45, 159, 264, 478, 585
PspPI GGNCC 2 cut(s) 43, 584
RsaI GTAC 2 cut(s) 635, 686
RsaNI GTAC 2 cut(s) 634, 685
SacII CCGCGG 1 cut(s) 70
SaqAI TTAA 4 cut(s) 282, 543, 735, 754
SatI GCNGC 3 cut(s) 70, 90, 499
Sau3AI GATC 6 cut(s) 15, 26, 244, 370, 565, 690
Sau96I GGNCC 2 cut(s) 43, 584
ScaI AGTACT 1 cut(s) 635
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 2 cut(s) 58, 300
SduI GDGCHC 1 cut(s) 152
SetI ASST 5 cut(s) 122, 264, 391, 500, 536
SfaNI GCATC 1 cut(s) 450
SfoI GGCGCC 1 cut(s) 478
Sfr303I CCGCGG 1 cut(s) 70
SgrBI CCGCGG 1 cut(s) 70
SmlI CTYRAG 1 cut(s) 734
SmoI CTYRAG 1 cut(s) 734
Sse9I AATT 2 cut(s) 525, 670
SsiI CCGC 4 cut(s) 67, 69, 90, 102
SspDI GGCGCC 1 cut(s) 476
SspMI CTAG 3 cut(s) 50, 374, 488
StyD4I CCNGG 2 cut(s) 56, 298
StyI CCWWGG 2 cut(s) 180, 465
TaaI ACNGT 1 cut(s) 313
TaqI TCGA 3 cut(s) 14, 210, 437
TaqII GACCGA 1 cut(s) 254
TasI AATT 2 cut(s) 525, 670
TatI WGTACW 2 cut(s) 633, 684
TauI GCSGC 2 cut(s) 72, 92
TfiI GAWTC 5 cut(s) 220, 346, 547, 610, 651
Tru1I TTAA 4 cut(s) 282, 543, 735, 754
Tru9I TTAA 4 cut(s) 282, 543, 735, 754
TscAI CASTG 1 cut(s) 230
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 1 cut(s) 498
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 1 cut(s) 354
TspRI CASTG 1 cut(s) 230
Van91I CCANNNNNTGG 1 cut(s) 601
Vha464I CTTAAG 1 cut(s) 734
XbaI TCTAGA 1 cut(s) 487
XceI RCATGY 1 cut(s) 367
XmnI GAANNNNTTC 3 cut(s) 261, 345, 623
XspI CTAG 3 cut(s) 50, 374, 488
ZrmI AGTACT 1 cut(s) 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.