MD01G1140100.v1.1

protein At2g39795, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008278
Physical Location & Seq
Forward (+)
24953417 .. 24955254
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1140100.v1.1.491

Sequence Viewer

Length: 762 bp
ATGGCTTTCACTTCAATTCTCCGCCGATCGGCCTTCTCCCTGGCCCCACTCGCCAGCCGCCTCGCTCGCGGCCAGAGATCTTACCACGGCGCTGTTGCCACCGCCGCCAACCACTTCAATTTCTCCCGCAAGCCCACTCAACCGAGCCCTTTCGTTCCCACCCATCGATACTACTCGAGCCACAGTTCGGACCAGTCTCTGATCAGGGTCATCGAGTCGGAGATCAAGTGCGCTGAGGAGACCGATGATCTCGACAAGGTTGAGGAGGTTCCATCATCCTTCCCTTTTAAAATTGAAGATACTCCCGGAATCCAAACAGTGACGCTGAAAAGAACATATCAAGGTGAAGACATACAGGTTGAAGTTCACATGCCTGATCTAGTAACTGGTGAGGACGACGACGATGATAACCAGGATGATGACAACGATGAACACGCCAACAAATCCAGCATCCCGTTGGTCGTAACTGTCTCCAAGACTGATGGACCAGTTCTCGAGTTTAGCGTCACTGCTTACCCTGATGAGTTTCAAATTGACAGCTTGGCAGTGAAAAATCCAGAGGATTCTGAGGATCAAATCGCCTACGAGGGGCCTGACTTCCACGATTTGGATGAGAACCTTCAGAAGGCATTCCACAAGTACTTGGAGGTGAGAGGGATCAAGCCCAGCACAACCAATTTCTTGCATGAGTACATGCTCAGCAAGGACAGCAAAGAATATGCAAACTGGTTGCAGCAACTTAAGAGCTTCGTCGAAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
MAM33

Protein Analysis

254

Amino Acids

28.61

Weight (kDa)

4.64

Isoelectric Point (pI)

49.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAM33 PF02330 71 - 251 1.6e-52 Mitochondrial glycoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 607
AccII CGCG 1 cut(s) 69
AciI CCGC 6 cut(s) 22, 58, 69, 102, 105, 127
AclWI GGATC 2 cut(s) 579, 665
AcoI YGGCCR 1 cut(s) 70
AcuI CTGAAG 1 cut(s) 605
AfaI GTAC 2 cut(s) 641, 692
AfiI CCNNNNNNNGG 5 cut(s) 28, 187, 588, 607, 625
AflII CTTAAG 1 cut(s) 740
AgsI TTSAA 5 cut(s) 15, 118, 296, 362, 530
AjnI CCWGG 2 cut(s) 39, 411
AluBI AGCT 2 cut(s) 540, 747
AluI AGCT 2 cut(s) 540, 747
Alw26I GTCTC 3 cut(s) 201, 233, 475
AlwI GGATC 2 cut(s) 579, 665
AlwNI CAGNNNCTG 1 cut(s) 199
Ama87I CYCGRG 2 cut(s) 175, 494
AoxI GGCC 4 cut(s) 30, 42, 70, 590
ApeKI GCWGC 1 cut(s) 733
Asp700I GAANNNNTTC 1 cut(s) 629
AspLEI GCGC 2 cut(s) 92, 233
AspS9I GGNCC 4 cut(s) 43, 190, 485, 590
AsuC2I CCSGG 1 cut(s) 306
AsuHPI GGTGA 3 cut(s) 356, 401, 661
AvaI CYCGRG 2 cut(s) 175, 494
AvaII GGWCC 2 cut(s) 190, 485
BanII GRGCYC 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 354
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 745
BccI CCATC 3 cut(s) 171, 280, 476
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 2 cut(s) 41, 413
BclI TGATCA 1 cut(s) 201
BcnI CCSGG 1 cut(s) 306
BcoDI GTCTC 3 cut(s) 201, 233, 475
BfaI CTAG 1 cut(s) 380
BfoI RGCGCY 1 cut(s) 93
BfrI CTTAAG 1 cut(s) 740
BglII AGATCT 1 cut(s) 77
BisI GCNGC 4 cut(s) 58, 70, 105, 734
BlpI GCTNAGC 1 cut(s) 698
BlsI GCNGC 4 cut(s) 59, 71, 106, 735
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 3 cut(s) 41, 306, 413
Bme18I GGWCC 2 cut(s) 190, 485
BmeT110I CYCGRG 2 cut(s) 175, 494
BmgT120I GGNCC 4 cut(s) 43, 190, 485, 590
BmiI GGNNCC 3 cut(s) 45, 270, 591
BmrFI CCNGG 3 cut(s) 41, 306, 413
BmsI GCATC 1 cut(s) 459
BpiI GAAGAC 1 cut(s) 354
Bpu10I CCTNAGC 1 cut(s) 234
Bpu1102I GCTNAGC 1 cut(s) 698
BpuMI CCSGG 1 cut(s) 306
Bsa29I ATCGAT 1 cut(s) 166
BsaI GGTCTC 1 cut(s) 233
BsaJI CCNNGG 2 cut(s) 39, 85
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 187, 588, 607, 625
Bse1I ACTGG 4 cut(s) 193, 391, 488, 731
BseBI CCWGG 2 cut(s) 41, 413
BseCI ATCGAT 1 cut(s) 166
BseDI CCNNGG 2 cut(s) 39, 85
BseGI GGATG 4 cut(s) 275, 421, 450, 616
BseLI CCNNNNNNNGG 5 cut(s) 28, 187, 588, 607, 625
BseMII CTCAG 3 cut(s) 225, 558, 712
BseNI ACTGG 4 cut(s) 193, 391, 488, 731
BseRI GAGGAG 2 cut(s) 251, 278
BseXI GCAGC 1 cut(s) 745
BseYI CCCAGC 1 cut(s) 665
Bsh1236I CGCG 1 cut(s) 69
Bsh1285I CGRYCG 1 cut(s) 29
BshFI GGCC 4 cut(s) 32, 44, 72, 592
BshVI ATCGAT 1 cut(s) 166
BsiEI CGRYCG 1 cut(s) 29
BsiHKCI CYCGRG 2 cut(s) 175, 494
BsiSI CCGG 1 cut(s) 306
BslI CCNNNNNNNGG 5 cut(s) 28, 187, 588, 607, 625
BsmAI GTCTC 3 cut(s) 201, 233, 475
BsmI GAATGC 1 cut(s) 629
BsnI GGCC 4 cut(s) 32, 44, 72, 592
Bso31I GGTCTC 1 cut(s) 233
BsoBI CYCGRG 2 cut(s) 175, 494
Bsp1286I GDGCHC 1 cut(s) 149
Bsp143I GATC 8 cut(s) 26, 77, 201, 222, 247, 376, 571, 657
Bsp1720I GCTNAGC 1 cut(s) 698
BspACI CCGC 6 cut(s) 22, 58, 69, 102, 105, 127
BspANI GGCC 4 cut(s) 32, 44, 72, 592
BspCNI CTCAG 3 cut(s) 226, 559, 711
BspDI ATCGAT 1 cut(s) 166
BspFNI CGCG 1 cut(s) 69
BspLI GGNNCC 3 cut(s) 45, 270, 591
BspPI GGATC 2 cut(s) 579, 665
BspTI CTTAAG 1 cut(s) 740
BspTNI GGTCTC 1 cut(s) 233
BsrI ACTGG 4 cut(s) 193, 391, 488, 731
BssECI CCNNGG 2 cut(s) 39, 85
BssMI GATC 8 cut(s) 26, 77, 201, 222, 247, 376, 571, 657
Bst2UI CCWGG 2 cut(s) 41, 413
Bst4CI ACNGT 3 cut(s) 185, 319, 469
BstAFI CTTAAG 1 cut(s) 740
BstC8I GCNNGC 3 cut(s) 55, 67, 131
BstDEI CTNAG 3 cut(s) 234, 567, 698
BstDSI CCRYGG 1 cut(s) 85
BstENI CCTNNNNNAGG 1 cut(s) 623
BstF5I GGATG 4 cut(s) 275, 421, 450, 616
BstFNI CGCG 1 cut(s) 69
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 2 cut(s) 92, 233
BstKTI GATC 8 cut(s) 29, 80, 204, 225, 250, 379, 574, 660
BstMAI GTCTC 3 cut(s) 201, 233, 475
BstMBI GATC 8 cut(s) 26, 77, 201, 222, 247, 376, 571, 657
BstMCI CGRYCG 1 cut(s) 29
BstMWI GCNNNNNNNGC 4 cut(s) 50, 66, 104, 708
BstNI CCWGG 2 cut(s) 41, 413
BstNSI RCATGY 2 cut(s) 373, 697
BstSCI CCNGG 3 cut(s) 39, 304, 411
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 1 cut(s) 745
BstV2I GAAGAC 1 cut(s) 354
BstX2I RGATCY 1 cut(s) 77
BstYI RGATCY 1 cut(s) 77
Bsu15I ATCGAT 1 cut(s) 166
BsuRI GGCC 4 cut(s) 32, 44, 72, 592
BsuTUI ATCGAT 1 cut(s) 166
BtgI CCRYGG 1 cut(s) 85
BtsCI GGATG 4 cut(s) 275, 421, 450, 616
BtsI GCAGTG 2 cut(s) 507, 552
BtsIMutI CAGTG 3 cut(s) 324, 507, 552
Cac8I GCNNGC 3 cut(s) 55, 67, 131
CaiI CAGNNNCTG 1 cut(s) 199
CfoI GCGC 2 cut(s) 92, 233
Cfr13I GGNCC 4 cut(s) 43, 190, 485, 590
ClaI ATCGAT 1 cut(s) 166
CseI GACGC 2 cut(s) 331, 493
Csp6I GTAC 2 cut(s) 640, 691
CviAII CATG 3 cut(s) 370, 686, 694
CviQI GTAC 2 cut(s) 640, 691
DdeI CTNAG 3 cut(s) 234, 567, 698
DpnI GATC 8 cut(s) 28, 79, 203, 224, 249, 378, 573, 659
DpnII GATC 8 cut(s) 26, 77, 201, 222, 247, 376, 571, 657
DraI TTTAAA 1 cut(s) 289
EaeI YGGCCR 1 cut(s) 70
EciI GGCGGA 1 cut(s) 11
Eco24I GRGCYC 1 cut(s) 149
Eco31I GGTCTC 1 cut(s) 233
Eco47I GGWCC 2 cut(s) 190, 485
Eco57I CTGAAG 1 cut(s) 605
Eco88I CYCGRG 2 cut(s) 175, 494
EcoNI CCTNNNNNAGG 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 590
EcoRII CCWGG 2 cut(s) 39, 411
EcoT38I GRGCYC 1 cut(s) 149
FaeI CATG 3 cut(s) 373, 689, 697
FaiI YATR 6 cut(s) 337, 353, 371, 687, 695, 720
FatI CATG 3 cut(s) 369, 685, 693
FauI CCCGC 1 cut(s) 134
FbaI TGATCA 1 cut(s) 201
Fnu4HI GCNGC 4 cut(s) 58, 70, 105, 734
FokI GGATG 4 cut(s) 262, 428, 437, 623
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 4 cut(s) 58, 70, 105, 734
FspBI CTAG 1 cut(s) 380
GlaI GCGC 2 cut(s) 91, 232
GluI GCNGC 4 cut(s) 58, 70, 105, 734
GsaI CCCAGC 1 cut(s) 669
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 4 cut(s) 32, 44, 72, 592
HapII CCGG 1 cut(s) 306
HgaI GACGC 2 cut(s) 331, 493
HhaI GCGC 2 cut(s) 92, 233
Hin1II CATG 3 cut(s) 373, 689, 697
Hin6I GCGC 2 cut(s) 90, 231
HinP1I GCGC 2 cut(s) 90, 231
HinfI GANTC 3 cut(s) 215, 309, 563
HpaII CCGG 1 cut(s) 306
HphI GGTGA 3 cut(s) 356, 401, 661
Hpy166II GTNNAC 1 cut(s) 367
Hpy188I TCNGA 5 cut(s) 190, 201, 220, 568, 624
Hpy188III TCNNGA 3 cut(s) 251, 494, 557
Hpy8I GTNNAC 1 cut(s) 367
Hpy99I CGWCG 3 cut(s) 401, 404, 755
HpyAV CCTTC 4 cut(s) 43, 289, 619, 629
HpyCH4III ACNGT 3 cut(s) 185, 319, 469
HpyCH4V TGCA 3 cut(s) 685, 722, 733
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 66, 104, 708
HpyF3I CTNAG 3 cut(s) 234, 567, 698
Hsp92II CATG 3 cut(s) 373, 689, 697
HspAI GCGC 2 cut(s) 90, 231
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 8 cut(s) 26, 77, 201, 222, 247, 376, 571, 657
Lsp1109I GCAGC 1 cut(s) 745
LweI GCATC 1 cut(s) 459
MaeI CTAG 1 cut(s) 380
MaeIII GTNAC 4 cut(s) 319, 382, 463, 505
MalI GATC 8 cut(s) 28, 79, 203, 224, 249, 378, 573, 659
MboI GATC 8 cut(s) 26, 77, 201, 222, 247, 376, 571, 657
MboII GAAGA 2 cut(s) 308, 359
MflI RGATCY 1 cut(s) 77
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 5 cut(s) 15, 118, 291, 531, 676
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 198
MroXI GAANNNNTTC 1 cut(s) 629
MseI TTAA 2 cut(s) 288, 741
MspCI CTTAAG 1 cut(s) 740
MspI CCGG 1 cut(s) 306
MspR9I CCNGG 3 cut(s) 41, 306, 413
Mva1269I GAATGC 1 cut(s) 629
MvaI CCWGG 2 cut(s) 41, 413
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 4 cut(s) 50, 66, 104, 708
NciI CCSGG 1 cut(s) 306
NdeII GATC 8 cut(s) 26, 77, 201, 222, 247, 376, 571, 657
NlaIII CATG 3 cut(s) 373, 689, 697
NlaIV GGNNCC 3 cut(s) 45, 270, 591
NmuCI GTSAC 2 cut(s) 319, 505
NspI RCATGY 2 cut(s) 373, 697
PaeR7I CTCGAG 2 cut(s) 175, 494
PcsI WCGNNNNNNNCGW 1 cut(s) 501
PctI GAATGC 1 cut(s) 629
PdmI GAANNNNTTC 1 cut(s) 629
PfeI GAWTC 2 cut(s) 309, 563
PflMI CCANNNNNTGG 1 cut(s) 607
PfoI TCCNGGA 1 cut(s) 304
PkrI GCNGC 4 cut(s) 59, 71, 106, 735
Ple19I CGATCG 1 cut(s) 29
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
Psp6I CCWGG 2 cut(s) 39, 411
PspFI CCCAGC 1 cut(s) 665
PspGI CCWGG 2 cut(s) 39, 411
PspN4I GGNNCC 3 cut(s) 45, 270, 591
PspPI GGNCC 4 cut(s) 43, 190, 485, 590
PspXI VCTCGAGB 1 cut(s) 175
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 77
PvuI CGATCG 1 cut(s) 29
RsaI GTAC 2 cut(s) 641, 692
RsaNI GTAC 2 cut(s) 640, 691
SaqAI TTAA 2 cut(s) 288, 741
SatI GCNGC 4 cut(s) 58, 70, 105, 734
Sau3AI GATC 8 cut(s) 26, 77, 201, 222, 247, 376, 571, 657
Sau96I GGNCC 4 cut(s) 43, 190, 485, 590
ScaI AGTACT 1 cut(s) 641
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 3 cut(s) 41, 306, 413
SduI GDGCHC 1 cut(s) 149
SetI ASST 8 cut(s) 261, 270, 346, 360, 542, 621, 651, 749
SfaNI GCATC 1 cut(s) 459
Sfr274I CTCGAG 2 cut(s) 175, 494
SinI GGWCC 2 cut(s) 190, 485
SlaI CTCGAG 2 cut(s) 175, 494
SmlI CTYRAG 3 cut(s) 175, 494, 740
SmoI CTYRAG 3 cut(s) 175, 494, 740
Sse9I AATT 5 cut(s) 15, 118, 291, 531, 676
SsiI CCGC 6 cut(s) 22, 58, 69, 102, 105, 127
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 3 cut(s) 39, 304, 411
TaaI ACNGT 3 cut(s) 185, 319, 469
TaqI TCGA 6 cut(s) 166, 176, 213, 252, 495, 753
TaqII GACCGA 1 cut(s) 257
TasI AATT 5 cut(s) 15, 118, 291, 531, 676
TatI WGTACW 2 cut(s) 639, 690
TauI GCSGC 3 cut(s) 60, 72, 107
TfiI GAWTC 2 cut(s) 309, 563
Tru1I TTAA 2 cut(s) 288, 741
Tru9I TTAA 2 cut(s) 288, 741
TscAI CASTG 3 cut(s) 324, 514, 552
TseFI GTSAC 2 cut(s) 319, 505
TseI GCWGC 1 cut(s) 733
Tsp45I GTSAC 2 cut(s) 319, 505
TspDTI ATGAA 1 cut(s) 444
TspRI CASTG 3 cut(s) 324, 514, 552
Van91I CCANNNNNTGG 1 cut(s) 607
Vha464I CTTAAG 1 cut(s) 740
VpaK11BI GGWCC 2 cut(s) 190, 485
XagI CCTNNNNNAGG 1 cut(s) 623
XceI RCATGY 2 cut(s) 373, 697
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
XhoI CTCGAG 2 cut(s) 175, 494
XmnI GAANNNNTTC 1 cut(s) 629
XspI CTAG 1 cut(s) 380
ZrmI AGTACT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.