MD00G1150700.v1.1

protein At2g39795, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0008278
Physical Location & Seq
Reverse (-)
33236593 .. 33237354
762 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1150700.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGCTTTCACTTCAATTCTCCACCGATCGGCCTCCTCCCTGGCCCCACTCGCCAGCCGCCTTGCTCGTGGCCAGCGGTCTTACCACGGCGTTGTTGCCACCGCCGTCAACCGCTTCAATTTCTCCCGAAAGCACACTCAGCCGACCCCTTTCGTTCCCACCCCTCAATACTATTCGAGCCACAGTTCGGACCAGTCTCTGATCAGGGTGATCGAGTCGGAGATCAAGTGCGCTGAGGAGACCGAGGATCTTGACAAGGTTGAAGTTCACATGCCTGATCTAGTCACAGGTGAAGACGACAACGATGATAACCAAAATGATGACAATGATGAACACACCAACCAATCCAGCATCCCGTTGGTTGTAACTGTCTCCAAGGGTAACGGACCAGTTCTCGAGTTTAGCGTCACTGCTTACCCTGATGAGTTTCAAATTGACAGCTTGGCAGTGAAAAATCCTGAAGATTCCGAGGATCAAATTGCCTACGAGGGGCCTGATTTCCATGATTTGGATGAGAACCTTCAGAAAGCATTCCACAAGTATTTGGAGGTGAGAGGAATCAAGCCCAGCACAACTAAGTTCTTGCATGAGTACATGCTCAACAAGGACAGCAAAGAATATGCAAATTGGTTGCAGCAACTTAAGCGATTCGTCGAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko05168 map05168
Pfam Domains
Protein Families
MAM33

Protein Analysis

221

Amino Acids

24.99

Weight (kDa)

4.86

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAM33 PF02330 87 - 218 1.2e-36 Mitochondrial glycoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 508
AciI CCGC 4 cut(s) 58, 76, 102, 112
AclWI GGATC 2 cut(s) 255, 480
AcoI YGGCCR 1 cut(s) 70
AcuI CTGAAG 2 cut(s) 480, 506
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 4 cut(s) 28, 187, 489, 508
AflII CTTAAG 1 cut(s) 641
AgsI TTSAA 4 cut(s) 15, 118, 263, 431
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 1 cut(s) 441
AluI AGCT 1 cut(s) 441
Alw26I GTCTC 3 cut(s) 201, 233, 376
AlwI GGATC 2 cut(s) 255, 480
AlwNI CAGNNNCTG 1 cut(s) 199
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 4 cut(s) 30, 42, 70, 491
ApeKI GCWGC 1 cut(s) 634
Asp700I GAANNNNTTC 1 cut(s) 530
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 4 cut(s) 43, 190, 386, 491
AsuHPI GGTGA 3 cut(s) 220, 302, 562
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 2 cut(s) 190, 386
BalI TGGCCA 1 cut(s) 72
BauI CACGAG 1 cut(s) 66
BbsI GAAGAC 1 cut(s) 300
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 646
BceAI ACGGC 2 cut(s) 89, 103
BciT130I CCWGG 1 cut(s) 41
BclI TGATCA 1 cut(s) 201
BcoDI GTCTC 3 cut(s) 201, 233, 376
BfaI CTAG 1 cut(s) 281
BfrI CTTAAG 1 cut(s) 641
BisI GCNGC 2 cut(s) 58, 635
BlsI GCNGC 2 cut(s) 59, 636
Bme1390I CCNGG 1 cut(s) 41
Bme18I GGWCC 2 cut(s) 190, 386
BmeT110I CYCGRG 1 cut(s) 395
BmgT120I GGNCC 4 cut(s) 43, 190, 386, 491
BmiI GGNNCC 2 cut(s) 45, 492
BmrFI CCNGG 1 cut(s) 41
BmsI GCATC 1 cut(s) 360
BpiI GAAGAC 1 cut(s) 300
Bpu10I CCTNAGC 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 233
BsaJI CCNNGG 5 cut(s) 39, 85, 243, 375, 468
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 187, 489, 508
Bse1I ACTGG 2 cut(s) 193, 389
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 5 cut(s) 39, 85, 243, 375, 468
BseGI GGATG 2 cut(s) 351, 517
BseLI CCNNNNNNNGG 4 cut(s) 28, 187, 489, 508
BseMII CTCAG 2 cut(s) 152, 225
BseNI ACTGG 2 cut(s) 193, 389
BseRI GAGGAG 2 cut(s) 25, 251
BseXI GCAGC 1 cut(s) 646
BseYI CCCAGC 1 cut(s) 566
Bsh1285I CGRYCG 1 cut(s) 29
BshFI GGCC 4 cut(s) 32, 44, 72, 493
BsiEI CGRYCG 1 cut(s) 29
BsiHKCI CYCGRG 1 cut(s) 395
BslI CCNNNNNNNGG 4 cut(s) 28, 187, 489, 508
BsmAI GTCTC 3 cut(s) 201, 233, 376
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 4 cut(s) 32, 44, 72, 493
Bso31I GGTCTC 1 cut(s) 233
BsoBI CYCGRG 1 cut(s) 395
Bsp143I GATC 7 cut(s) 26, 201, 210, 222, 247, 277, 472
BspACI CCGC 4 cut(s) 58, 76, 102, 112
BspANI GGCC 4 cut(s) 32, 44, 72, 493
BspCNI CTCAG 2 cut(s) 151, 226
BspLI GGNNCC 2 cut(s) 45, 492
BspPI GGATC 2 cut(s) 255, 480
BspTI CTTAAG 1 cut(s) 641
BspTNI GGTCTC 1 cut(s) 233
BsrI ACTGG 2 cut(s) 193, 389
BssECI CCNNGG 5 cut(s) 39, 85, 243, 375, 468
BssMI GATC 7 cut(s) 26, 201, 210, 222, 247, 277, 472
BssSI CACGAG 1 cut(s) 66
BssT1I CCWWGG 1 cut(s) 375
Bst2BI CACGAG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 2 cut(s) 185, 370
BstAFI CTTAAG 1 cut(s) 641
BstC8I GCNNGC 2 cut(s) 55, 74
BstDEI CTNAG 3 cut(s) 138, 234, 576
BstDSI CCRYGG 1 cut(s) 85
BstF5I GGATG 2 cut(s) 351, 517
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 7 cut(s) 29, 204, 213, 225, 250, 280, 475
BstMAI GTCTC 3 cut(s) 201, 233, 376
BstMBI GATC 7 cut(s) 26, 201, 210, 222, 247, 277, 472
BstMCI CGRYCG 1 cut(s) 29
BstMWI GCNNNNNNNGC 3 cut(s) 50, 139, 643
BstNI CCWGG 1 cut(s) 41
BstNSI RCATGY 2 cut(s) 274, 598
BstSCI CCNGG 1 cut(s) 39
BstV1I GCAGC 1 cut(s) 646
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuRI GGCC 4 cut(s) 32, 44, 72, 493
BtgI CCRYGG 1 cut(s) 85
BtsCI GGATG 2 cut(s) 351, 517
BtsI GCAGTG 2 cut(s) 408, 453
BtsIMutI CAGTG 2 cut(s) 408, 453
Cac8I GCNNGC 2 cut(s) 55, 74
CaiI CAGNNNCTG 1 cut(s) 199
CfoI GCGC 1 cut(s) 233
Cfr13I GGNCC 4 cut(s) 43, 190, 386, 491
CseI GACGC 1 cut(s) 394
Csp6I GTAC 1 cut(s) 592
CviAII CATG 4 cut(s) 271, 503, 587, 595
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 3 cut(s) 138, 234, 576
DpnI GATC 7 cut(s) 28, 203, 212, 224, 249, 279, 474
DpnII GATC 7 cut(s) 26, 201, 210, 222, 247, 277, 472
EaeI YGGCCR 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 375
Eco31I GGTCTC 1 cut(s) 233
Eco47I GGWCC 2 cut(s) 190, 386
Eco57I CTGAAG 2 cut(s) 480, 506
Eco88I CYCGRG 1 cut(s) 395
EcoO109I RGGNCCY 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 39
EcoT14I CCWWGG 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 4 cut(s) 274, 506, 590, 598
FaiI YATR 6 cut(s) 272, 504, 588, 596, 621, 661
FatI CATG 4 cut(s) 270, 502, 586, 594
FbaI TGATCA 1 cut(s) 201
Fnu4HI GCNGC 2 cut(s) 58, 635
FokI GGATG 2 cut(s) 338, 524
Fsp4HI GCNGC 2 cut(s) 58, 635
FspBI CTAG 1 cut(s) 281
GlaI GCGC 1 cut(s) 232
GluI GCNGC 2 cut(s) 58, 635
GsaI CCCAGC 1 cut(s) 570
HaeIII GGCC 4 cut(s) 32, 44, 72, 493
HgaI GACGC 1 cut(s) 394
HhaI GCGC 1 cut(s) 233
Hin1II CATG 4 cut(s) 274, 506, 590, 598
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 4 cut(s) 215, 464, 558, 648
HphI GGTGA 3 cut(s) 220, 302, 562
Hpy166II GTNNAC 2 cut(s) 109, 268
Hpy188I TCNGA 5 cut(s) 190, 201, 220, 469, 525
Hpy188III TCNNGA 4 cut(s) 126, 251, 395, 458
Hpy8I GTNNAC 2 cut(s) 109, 268
Hpy99I CGWCG 1 cut(s) 656
HpyAV CCTTC 1 cut(s) 530
HpyCH4III ACNGT 2 cut(s) 185, 370
HpyCH4V TGCA 3 cut(s) 586, 623, 634
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 139, 643
HpyF3I CTNAG 3 cut(s) 138, 234, 576
Hsp92II CATG 4 cut(s) 274, 506, 590, 598
HspAI GCGC 1 cut(s) 231
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 7 cut(s) 26, 201, 210, 222, 247, 277, 472
Lsp1109I GCAGC 1 cut(s) 646
LweI GCATC 1 cut(s) 360
MaeI CTAG 1 cut(s) 281
MaeIII GTNAC 4 cut(s) 283, 364, 380, 406
MalI GATC 7 cut(s) 28, 203, 212, 224, 249, 279, 474
MboI GATC 7 cut(s) 26, 201, 210, 222, 247, 277, 472
MboII GAAGA 2 cut(s) 305, 473
MflI RGATCY 1 cut(s) 247
MlsI TGGCCA 1 cut(s) 72
MluCI AATT 5 cut(s) 15, 118, 432, 477, 625
MluNI TGGCCA 1 cut(s) 72
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 9 cut(s) 43, 46, 174, 229, 238, 463, 481, 541, 548
Mox20I TGGCCA 1 cut(s) 72
MroXI GAANNNNTTC 1 cut(s) 530
MscI TGGCCA 1 cut(s) 72
MseI TTAA 1 cut(s) 642
Msp20I TGGCCA 1 cut(s) 72
MspA1I CMGCKG 1 cut(s) 76
MspCI CTTAAG 1 cut(s) 641
MspR9I CCNGG 1 cut(s) 41
Mva1269I GAATGC 1 cut(s) 530
MvaI CCWGG 1 cut(s) 41
MwoI GCNNNNNNNGC 3 cut(s) 50, 139, 643
NdeII GATC 7 cut(s) 26, 201, 210, 222, 247, 277, 472
NlaIII CATG 4 cut(s) 274, 506, 590, 598
NlaIV GGNNCC 2 cut(s) 45, 492
NmuCI GTSAC 2 cut(s) 283, 406
NspI RCATGY 2 cut(s) 274, 598
PaeR7I CTCGAG 1 cut(s) 395
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PctI GAATGC 1 cut(s) 530
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 3 cut(s) 464, 558, 648
PflMI CCANNNNNTGG 1 cut(s) 508
PkrI GCNGC 2 cut(s) 59, 636
Ple19I CGATCG 1 cut(s) 29
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
Psp6I CCWGG 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 45, 492
PspPI GGNCC 4 cut(s) 43, 190, 386, 491
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 247
PvuI CGATCG 1 cut(s) 29
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
SaqAI TTAA 1 cut(s) 642
SatI GCNGC 2 cut(s) 58, 635
Sau3AI GATC 7 cut(s) 26, 201, 210, 222, 247, 277, 472
Sau96I GGNCC 4 cut(s) 43, 190, 386, 491
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 41
SetI ASST 5 cut(s) 261, 292, 443, 522, 552
SfaNI GCATC 1 cut(s) 360
Sfr274I CTCGAG 1 cut(s) 395
SinI GGWCC 2 cut(s) 190, 386
SlaI CTCGAG 1 cut(s) 395
SmlI CTYRAG 2 cut(s) 395, 641
SmoI CTYRAG 2 cut(s) 395, 641
Sse9I AATT 5 cut(s) 15, 118, 432, 477, 625
SsiI CCGC 4 cut(s) 58, 76, 102, 112
SspMI CTAG 1 cut(s) 281
StyD4I CCNGG 1 cut(s) 39
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 2 cut(s) 185, 370
TaqI TCGA 4 cut(s) 176, 213, 396, 654
TaqII GACCGA 1 cut(s) 257
TasI AATT 5 cut(s) 15, 118, 432, 477, 625
TatI WGTACW 1 cut(s) 591
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 3 cut(s) 464, 558, 648
Tru1I TTAA 1 cut(s) 642
Tru9I TTAA 1 cut(s) 642
TscAI CASTG 2 cut(s) 415, 453
TseFI GTSAC 2 cut(s) 283, 406
TseI GCWGC 1 cut(s) 634
Tsp45I GTSAC 2 cut(s) 283, 406
TspDTI ATGAA 1 cut(s) 345
TspGWI ACGGA 1 cut(s) 399
TspRI CASTG 2 cut(s) 415, 453
Van91I CCANNNNNTGG 1 cut(s) 508
Vha464I CTTAAG 1 cut(s) 641
VpaK11BI GGWCC 2 cut(s) 190, 386
XceI RCATGY 2 cut(s) 274, 598
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XhoI CTCGAG 1 cut(s) 395
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.