FvH4_7g25500

sodium-coupled neutral amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0004145
Physical Location & Seq
Forward (+)
19437909 .. 19440032
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g25500.t1

Sequence Viewer

Length: 828 bp
ATGCTACCGTTGGTGTTGTTAAGGCGTGTAGAATCCCTTAGGTTCAGTTCGGCTATATCAGTTCTTCTTGCTGTAGTTTTTGTTGGGATAAGTTCTGTACTGGCAATCCTTGCAATCTTTGAAGGGAAAACCAAAACCCCAAGATTGGTTCCAAGTTTGGACCAGCAAACCACCTTCTTTGATCTGTTCACTGCTGTTCCAGTCATCGTGACGGCCTTCACTTTTCATTTCAATGTTCACCCTATTGGTTTTGAGCTCGGGAAGCCTTCGGATATGATCTTGGCTGTGCAAATCTCACTAGTGCTTTGTGCAGTCATCTACTTTACCATAGGACTATTTGGTTACCTATTGTTTGGGGACTCAATCATGACCGACATACTTATAAACTTCGATAGAAGCTCTGATTCAGCAATGGGGGCACTACTCAATGATGTGGTCAGATTGAGCTATGCACTCCACTTGATGTTGGTGTTTCCACTCTTGAACTTCTCTTTAAGAGCAAACTTGGATGAGTTTCTTTTCCCTAAGAGACAACTACTGACAACAGATACCAGAAGATTTATGTTCCTCACTATTGGTTTGCTAGTCTTTTCTTACCTAGCAGCAATAGCCTTCCCAAACATTTGGTATCTCTTTCAGTTTCTGGGTTCAACCTCTGCTGTTTGTCTTGCATTCATTTTCCCTGGTGCTATTGTTCTAAGGGATGTACATGGGATATCTACAAAGAGGGATACGATTTTGGCGACGATAATGGTAATCCTGTCAGTTGTTACAAGCGCAATTGCCATTTCCACCAATGTGTATAACACGTTCGGAAACAAGTCGTAA

Protein Analysis

276

Amino Acids

30.43

Weight (kDa)

8.6

Isoelectric Point (pI)

33.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 2 - 263 1.7e-31 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 383
AfaI GTAC 2 cut(s) 99, 708
AfiI CCNNNNNNNGG 1 cut(s) 145
AflIII ACRYGT 1 cut(s) 807
AgsI TTSAA 4 cut(s) 122, 232, 484, 651
AhlI ACTAGT 1 cut(s) 298
AjnI CCWGG 1 cut(s) 682
AleI CACNNNNGTG 1 cut(s) 797
AluBI AGCT 3 cut(s) 256, 399, 447
AluI AGCT 3 cut(s) 256, 399, 447
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 1 cut(s) 523
AlwNI CAGNNNCTG 1 cut(s) 643
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 1 cut(s) 213
ApeKI GCWGC 1 cut(s) 602
AspLEI GCGC 1 cut(s) 779
AspS9I GGNCC 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 230
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 1 cut(s) 160
AxyI CCTNAGG 1 cut(s) 38
BaeGI GKGCMC 1 cut(s) 421
BanII GRGCYC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 1 cut(s) 614
BceAI ACGGC 1 cut(s) 228
BciT130I CCWGG 1 cut(s) 684
BciVI GTATCC 1 cut(s) 724
BcoDI GTCTC 1 cut(s) 523
BcuI ACTAGT 1 cut(s) 298
BfaI CTAG 3 cut(s) 299, 584, 599
BfmI CTRYAG 1 cut(s) 72
BfuI GTATCC 1 cut(s) 724
BisI GCNGC 1 cut(s) 603
BlsI GCNGC 1 cut(s) 604
Bme1390I CCNGG 1 cut(s) 684
Bme18I GGWCC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 1 cut(s) 150
BmrFI CCNGG 1 cut(s) 684
BsaJI CCNNGG 1 cut(s) 682
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 2 cut(s) 105, 200
Bse21I CCTNAGG 1 cut(s) 38
Bse3DI GCAATG 1 cut(s) 417
BseBI CCWGG 1 cut(s) 684
BseDI CCNNGG 1 cut(s) 682
BseGI GGATG 2 cut(s) 514, 709
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMI GCAATG 1 cut(s) 417
BseNI ACTGG 2 cut(s) 105, 200
BseSI GKGCMC 1 cut(s) 421
BseXI GCAGC 1 cut(s) 614
BsgI GTGCAG 1 cut(s) 330
BshFI GGCC 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 258
BsiHKCI CYCGRG 1 cut(s) 257
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 1 cut(s) 523
BsmFI GGGAC 1 cut(s) 371
BsmI GAATGC 1 cut(s) 671
BsnI GGCC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 257
Bsp1286I GDGCHC 2 cut(s) 258, 421
Bsp1407I TGTACA 1 cut(s) 706
Bsp143I GATC 2 cut(s) 181, 276
BspANI GGCC 1 cut(s) 215
BspHI TCATGA 1 cut(s) 366
BspLI GGNNCC 1 cut(s) 150
BsrDI GCAATG 1 cut(s) 417
BsrGI TGTACA 1 cut(s) 706
BsrI ACTGG 2 cut(s) 105, 200
BssECI CCNNGG 1 cut(s) 682
BssMI GATC 2 cut(s) 181, 276
Bst2UI CCWGG 1 cut(s) 684
Bst4CI ACNGT 1 cut(s) 9
BstAPI GCANNNNNTGC 1 cut(s) 110
BstAUI TGTACA 1 cut(s) 706
BstDEI CTNAG 3 cut(s) 38, 525, 698
BstEII GGTNACC 1 cut(s) 341
BstF5I GGATG 2 cut(s) 514, 709
BstHHI GCGC 1 cut(s) 779
BstKTI GATC 2 cut(s) 184, 279
BstMAI GTCTC 1 cut(s) 523
BstMBI GATC 2 cut(s) 181, 276
BstMWI GCNNNNNNNGC 4 cut(s) 110, 262, 416, 608
BstNI CCWGG 1 cut(s) 684
BstPI GGTNACC 1 cut(s) 341
BstSCI CCNGG 1 cut(s) 682
BstSFI CTRYAG 1 cut(s) 72
BstSLI GKGCMC 1 cut(s) 421
BstV1I GCAGC 1 cut(s) 614
BstXI CCANNNNNNTGG 1 cut(s) 624
Bsu36I CCTNAGG 1 cut(s) 38
BsuI GTATCC 1 cut(s) 724
BsuRI GGCC 1 cut(s) 215
BtsCI GGATG 2 cut(s) 514, 709
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 189
CaiI CAGNNNCTG 1 cut(s) 643
CciI TCATGA 1 cut(s) 366
CfoI GCGC 1 cut(s) 779
Cfr13I GGNCC 1 cut(s) 160
Csp6I GTAC 2 cut(s) 98, 707
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviAII CATG 2 cut(s) 367, 710
CviJI RGCY 8 cut(s) 53, 215, 256, 265, 284, 399, 447, 611
CviKI_1 RGCY 8 cut(s) 53, 215, 256, 265, 284, 399, 447, 611
CviQI GTAC 2 cut(s) 98, 707
DdeI CTNAG 3 cut(s) 38, 525, 698
DpnI GATC 2 cut(s) 183, 278
DpnII GATC 2 cut(s) 181, 276
Ecl136II GAGCTC 1 cut(s) 256
Eco24I GRGCYC 1 cut(s) 258
Eco32I GATATC 1 cut(s) 717
Eco47I GGWCC 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 256
Eco81I CCTNAGG 1 cut(s) 38
Eco88I CYCGRG 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 256
EcoO65I GGTNACC 1 cut(s) 341
EcoRII CCWGG 1 cut(s) 682
EcoRV GATATC 1 cut(s) 717
EcoT38I GRGCYC 1 cut(s) 258
FaeI CATG 2 cut(s) 370, 713
FaqI GGGAC 1 cut(s) 371
FatI CATG 2 cut(s) 366, 709
Fnu4HI GCNGC 1 cut(s) 603
FokI GGATG 2 cut(s) 521, 716
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 1 cut(s) 603
FspBI CTAG 3 cut(s) 299, 584, 599
GlaI GCGC 1 cut(s) 778
GluI GCNGC 1 cut(s) 603
HaeIII GGCC 1 cut(s) 215
HhaI GCGC 1 cut(s) 779
Hin1II CATG 2 cut(s) 370, 713
Hin6I GCGC 1 cut(s) 777
HinP1I GCGC 1 cut(s) 777
HinfI GANTC 3 cut(s) 32, 359, 404
HphI GGTGA 1 cut(s) 230
Hpy166II GTNNAC 2 cut(s) 189, 238
Hpy188I TCNGA 4 cut(s) 271, 403, 440, 815
Hpy188III TCNNGA 4 cut(s) 208, 259, 367, 481
Hpy8I GTNNAC 2 cut(s) 189, 238
Hpy99I CGWCG 1 cut(s) 748
HpyAV CCTTC 5 cut(s) 116, 184, 226, 276, 622
HpyCH4III ACNGT 1 cut(s) 9
HpyCH4IV ACGT 1 cut(s) 809
HpyCH4V TGCA 5 cut(s) 113, 289, 311, 452, 671
HpyF10VI GCNNNNNNNGC 4 cut(s) 110, 262, 416, 608
HpyF3I CTNAG 3 cut(s) 38, 525, 698
HpySE526I ACGT 1 cut(s) 809
Hsp92II CATG 2 cut(s) 370, 713
HspAI GCGC 1 cut(s) 777
Kzo9I GATC 2 cut(s) 181, 276
LpnPI CCDG 8 cut(s) 86, 176, 213, 565, 629, 669, 696, 773
Lsp1109I GCAGC 1 cut(s) 614
MaeI CTAG 3 cut(s) 299, 584, 599
MaeII ACGT 1 cut(s) 809
MaeIII GTNAC 3 cut(s) 208, 341, 769
MalI GATC 2 cut(s) 183, 278
MboI GATC 2 cut(s) 181, 276
MboII GAAGA 2 cut(s) 56, 567
MfeI CAATTG 1 cut(s) 780
MhlI GDGCHC 2 cut(s) 258, 421
MluCI AATT 1 cut(s) 780
MlyI GAGTC 1 cut(s) 353
MnlI CCTC 3 cut(s) 578, 664, 720
MseI TTAA 2 cut(s) 20, 494
MslI CAYNNNNRTG 2 cut(s) 231, 797
MspR9I CCNGG 1 cut(s) 684
MunI CAATTG 1 cut(s) 780
Mva1269I GAATGC 1 cut(s) 671
MvaI CCWGG 1 cut(s) 684
MwoI GCNNNNNNNGC 4 cut(s) 110, 262, 416, 608
NdeII GATC 2 cut(s) 181, 276
NlaIII CATG 2 cut(s) 370, 713
NlaIV GGNNCC 1 cut(s) 150
NmuCI GTSAC 1 cut(s) 208
OliI CACNNNNGTG 1 cut(s) 797
PagI TCATGA 1 cut(s) 366
PcsI WCGNNNNNNNCGW 1 cut(s) 740
PctI GAATGC 1 cut(s) 671
PfeI GAWTC 2 cut(s) 32, 404
PkrI GCNGC 1 cut(s) 604
PleI GAGTC 1 cut(s) 353
PpsI GAGTC 1 cut(s) 353
PsiI TTATAA 1 cut(s) 383
Psp124BI GAGCTC 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 682
PspEI GGTNACC 1 cut(s) 341
PspGI CCWGG 1 cut(s) 682
PspN4I GGNNCC 1 cut(s) 150
PspPI GGNCC 1 cut(s) 160
PsrI GAACNNNNNNTAC 2 cut(s) 794, 826
PstNI CAGNNNCTG 1 cut(s) 643
RsaI GTAC 2 cut(s) 99, 708
RsaNI GTAC 2 cut(s) 98, 707
RseI CAYNNNNRTG 2 cut(s) 231, 797
SacI GAGCTC 1 cut(s) 258
SaqAI TTAA 2 cut(s) 20, 494
SatI GCNGC 1 cut(s) 603
Sau3AI GATC 2 cut(s) 181, 276
Sau96I GGNCC 1 cut(s) 160
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 1 cut(s) 684
SduI GDGCHC 2 cut(s) 258, 421
SetI ASST 9 cut(s) 44, 176, 258, 348, 401, 449, 600, 656, 812
SfcI CTRYAG 1 cut(s) 72
SinI GGWCC 1 cut(s) 160
SmiMI CAYNNNNRTG 2 cut(s) 231, 797
SpeI ACTAGT 1 cut(s) 298
Sse9I AATT 1 cut(s) 780
SspMI CTAG 3 cut(s) 299, 584, 599
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 1 cut(s) 682
TaaI ACNGT 1 cut(s) 9
TaiI ACGT 1 cut(s) 812
TaqI TCGA 1 cut(s) 390
TaqII GACCGA 1 cut(s) 386
TasI AATT 1 cut(s) 780
TatI WGTACW 2 cut(s) 97, 706
TfiI GAWTC 2 cut(s) 32, 404
Tru1I TTAA 2 cut(s) 20, 494
Tru9I TTAA 2 cut(s) 20, 494
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 1 cut(s) 602
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 2 cut(s) 215, 664
TspRI CASTG 1 cut(s) 196
VpaK11BI GGWCC 1 cut(s) 160
XspI CTAG 3 cut(s) 299, 584, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.