Rw1G032920

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004145
Physical Location & Seq
Forward (+)
61111306 .. 61113769
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032920.1

Sequence Viewer

Length: 1311 bp
ATGACGAACCAGCAAAACAACGATGAAGCCGGAGTGGTCACTCATCCTCTTCTCCATGACTTCAAAACCTTAGAAGGTCAAACTTCTTCATCATCCTCCATACAAGGCGCTTCATTTTCCGGCGCAGTTTTCAACATCTCAACCACCATGATCGGAGCCGGAATCATGTCTATCCCGGCCACCATGAAAGTGCTGGGAATAATTCCCGGGTTCATAGCTATCTTAGTAGTGGCTGTACACGAACTCCGGCAAGTCGGAAACTACGCCGGCATGGTGGGGGAGTCGTTTGGACCGTTGGGTTCGATTGCTGTGCAAGTCTGTATCATCATAACCAACCTGGGTTGCCTCATCATTTATTTCATCATCATCGGGGATGTGCTATGTGGGAGTACTCAATCTGGAGGAATTTTGCATTTGGGAATGTTACAAGAATGGTTTGGCATCCATTGGTGGAATTCTCGAGCTTATGCGCTTCTTTTCATTGCTATATTTGTGATGCTTCCATTAGTCTTGTTACGCCGAGTAGATTCACTTAGGCACACTTCGGCAATATCCATTTTGTTAGCAGTGGTTTTTGTTGCCATATGCTCTGCAACGGCGATTTATGCTGTGTGCAAAGGGAGAGCTCACAAACTGAGACTATATCCGGACTTTGCAATGGTCTCAATATTTGATATTTTCACCAGTATCCCAGTCGTTGTGACAAGTTTTGGATTCCATGTCAATGTTCATCCAATTAGAGCAGAGCTCGGTGAGCCTTCTGATATGAAGTTAGCTGGTCGGATTTCTCTGCTGATCTGCGTCACCATTTACTTCTCGATTGGCTTCTTTGGCTACCTATTGTTCGGGGACTCAATAATGGCTGACATGCTAGTAAACTTTGATCAAAACTCTGGCTCCACATTCGGGCAGATGCTCAATGTTGTTGTTCGATCAAGCTATGCAATGCACCTCTTGCTGGTGTTCCCCGTTATGCACTTTTCATTAAGGGCCAACATAGATGAACTGCTCTTCCCAAACAAGCCTGTTTTGGCGGAAAGCAACTCAAGATTTCTAATCCTCACTTGTGTCTTGCTCTCATTTTCTTACTTTGTGGCAATAGCTATCCCAAACATATGGTACTTCTTTCAGTTTATGGGATCAACCACTGTGGTTTGCCTCTCATTTATATTCCCTGGTGCTCTCATTCTCAGGGATGGACATGGGATATCAACAACAAAGGACAAGATAATAGCAATATTGCTATATCTATCAATCTATGTTCCTCTTGAAATGACCCCTCCACCACTTACCTCCAAAAAGAGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

436

Amino Acids

47.75

Weight (kDa)

8.09

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 37 - 395 1.1e-43 Transmembrane amino acid transporter protein
Trp_Tyr_perm PF03222 38 - 252 9e-08 Tryptophan/tyrosine permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 646
AciI CCGC 1 cut(s) 1034
AclWI GGATC 1 cut(s) 1147
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 2 cut(s) 405, 454
AfaI GTAC 3 cut(s) 237, 391, 1121
AfiI CCNNNNNNNGG 2 cut(s) 906, 958
AgsI TTSAA 3 cut(s) 64, 133, 1271
AjnI CCWGG 2 cut(s) 336, 1174
AloI GAACNNNNNNTCC 2 cut(s) 996, 1028
AluBI AGCT 7 cut(s) 218, 464, 626, 748, 776, 939, 1103
AluI AGCT 7 cut(s) 218, 464, 626, 748, 776, 939, 1103
Alw21I GWGCWC 3 cut(s) 628, 750, 1183
Alw26I GTCTC 2 cut(s) 631, 667
AlwI GGATC 1 cut(s) 1147
Ama87I CYCGRG 2 cut(s) 206, 459
Aor13HI TCCGGA 1 cut(s) 646
AoxI GGCC 2 cut(s) 177, 990
ApoI RAATTY 2 cut(s) 405, 454
AspLEI GCGC 3 cut(s) 110, 125, 472
AspS9I GGNCC 2 cut(s) 290, 990
AsuC2I CCSGG 3 cut(s) 176, 207, 208
AsuHPI GGTGA 3 cut(s) 673, 764, 796
AvaI CYCGRG 2 cut(s) 206, 459
AvaII GGWCC 1 cut(s) 290
BanII GRGCYC 2 cut(s) 628, 750
Bbv12I GWGCWC 3 cut(s) 628, 750, 1183
BccI CCATC 1 cut(s) 1190
BceAI ACGGC 1 cut(s) 612
BcgI CGANNNNNNTGC 2 cut(s) 292, 326
BciT130I CCWGG 2 cut(s) 338, 1176
BciVI GTATCC 1 cut(s) 698
BclI TGATCA 1 cut(s) 883
BcnI CCSGG 3 cut(s) 176, 207, 208
BcoDI GTCTC 2 cut(s) 631, 667
BfaI CTAG 1 cut(s) 872
BfoI RGCGCY 1 cut(s) 111
BfuI GTATCC 1 cut(s) 698
BmcAI AGTACT 1 cut(s) 391
Bme1390I CCNGG 5 cut(s) 176, 207, 208, 338, 1176
Bme18I GGWCC 1 cut(s) 290
BmeT110I CYCGRG 2 cut(s) 206, 459
BmgT120I GGNCC 2 cut(s) 290, 990
BmiI GGNNCC 2 cut(s) 157, 898
BmrFI CCNGG 5 cut(s) 176, 207, 208, 338, 1176
BmrI ACTGGG 1 cut(s) 686
BmsI GCATC 3 cut(s) 450, 486, 903
BmuI ACTGGG 1 cut(s) 686
BplI GAGNNNNNCTC 2 cut(s) 732, 764
BpmI CTGGAG 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 1030
BpuMI CCSGG 3 cut(s) 176, 207, 208
BsaI GGTCTC 1 cut(s) 667
BsaJI CCNNGG 3 cut(s) 206, 337, 1174
BsaWI WCCGGW 1 cut(s) 646
BsaXI ACNNNNNCTCC 4 cut(s) 228, 258, 881, 911
Bsc4I CCNNNNNNNGG 2 cut(s) 906, 958
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 2 cut(s) 684, 692
Bse3DI GCAATG 3 cut(s) 480, 663, 951
BseAI TCCGGA 1 cut(s) 646
BseBI CCWGG 2 cut(s) 338, 1176
BseDI CCNNGG 3 cut(s) 206, 337, 1174
BseGI GGATG 6 cut(s) 43, 92, 379, 441, 730, 1201
BseLI CCNNNNNNNGG 2 cut(s) 906, 958
BseMI GCAATG 3 cut(s) 480, 663, 951
BseMII CTCAG 2 cut(s) 626, 1204
BseNI ACTGG 2 cut(s) 684, 692
BseYI CCCAGC 1 cut(s) 193
BshFI GGCC 2 cut(s) 179, 992
BsiHKAI GWGCWC 3 cut(s) 628, 750, 1183
BsiHKCI CYCGRG 2 cut(s) 206, 459
BsiSI CCGG 8 cut(s) 30, 120, 159, 176, 207, 247, 267, 647
BslFI GGGAC 1 cut(s) 863
BslI CCNNNNNNNGG 2 cut(s) 906, 958
BsmAI GTCTC 2 cut(s) 631, 667
BsmFI GGGAC 1 cut(s) 863
BsnI GGCC 2 cut(s) 179, 992
Bso31I GGTCTC 1 cut(s) 667
BsoBI CYCGRG 2 cut(s) 206, 459
Bsp1286I GDGCHC 3 cut(s) 628, 750, 1183
Bsp13I TCCGGA 1 cut(s) 646
Bsp1407I TGTACA 1 cut(s) 235
Bsp143I GATC 5 cut(s) 150, 795, 883, 932, 1139
BspACI CCGC 1 cut(s) 1034
BspANI GGCC 2 cut(s) 179, 992
BspCNI CTCAG 2 cut(s) 627, 1203
BspEI TCCGGA 1 cut(s) 646
BspLI GGNNCC 2 cut(s) 157, 898
BspPI GGATC 1 cut(s) 1147
BspQI GCTCTTC 1 cut(s) 1016
BspTNI GGTCTC 1 cut(s) 667
BsrDI GCAATG 3 cut(s) 480, 663, 951
BsrFI RCCGGY 1 cut(s) 266
BsrGI TGTACA 1 cut(s) 235
BsrI ACTGG 2 cut(s) 684, 692
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 3 cut(s) 206, 337, 1174
BssMI GATC 5 cut(s) 150, 795, 883, 932, 1139
Bst2UI CCWGG 2 cut(s) 338, 1176
Bst4CI ACNGT 2 cut(s) 294, 1150
Bst6I CTCTTC 2 cut(s) 54, 1016
BstAPI GCANNNNNTGC 1 cut(s) 955
BstAUI TGTACA 1 cut(s) 235
BstC8I GCNNGC 1 cut(s) 268
BstDEI CTNAG 5 cut(s) 70, 223, 533, 635, 1190
BstF5I GGATG 6 cut(s) 43, 92, 379, 441, 730, 1201
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 3 cut(s) 110, 125, 472
BstKTI GATC 5 cut(s) 153, 798, 886, 935, 1142
BstMAI GTCTC 2 cut(s) 631, 667
BstMBI GATC 5 cut(s) 150, 795, 883, 932, 1139
BstMWI GCNNNNNNNGC 4 cut(s) 605, 754, 831, 955
BstNI CCWGG 2 cut(s) 338, 1176
BstNSI RCATGY 1 cut(s) 871
BstSCI CCNGG 5 cut(s) 174, 205, 206, 336, 1174
BstXI CCANNNNNNTGG 1 cut(s) 1116
BsuI GTATCC 1 cut(s) 698
BsuRI GGCC 2 cut(s) 179, 992
BtsCI GGATG 6 cut(s) 43, 92, 379, 441, 730, 1201
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 2 cut(s) 573, 1146
Cac8I GCNNGC 1 cut(s) 268
CfoI GCGC 3 cut(s) 110, 125, 472
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 2 cut(s) 290, 990
Cfr9I CCCGGG 1 cut(s) 206
CseI GACGC 1 cut(s) 790
Csp6I GTAC 3 cut(s) 236, 390, 1120
CspCI CAANNNNNGTGG 2 cut(s) 1131, 1166
CviAII CATG 8 cut(s) 56, 148, 166, 184, 271, 719, 868, 1202
CviQI GTAC 3 cut(s) 236, 390, 1120
DdeI CTNAG 5 cut(s) 70, 223, 533, 635, 1190
DpnI GATC 5 cut(s) 152, 797, 885, 934, 1141
DpnII GATC 5 cut(s) 150, 795, 883, 932, 1139
EaeI YGGCCR 1 cut(s) 177
Eam1104I CTCTTC 2 cut(s) 54, 1016
EarI CTCTTC 2 cut(s) 54, 1016
EciI GGCGGA 1 cut(s) 1049
Ecl136II GAGCTC 2 cut(s) 626, 748
Eco24I GRGCYC 2 cut(s) 628, 750
Eco31I GGTCTC 1 cut(s) 667
Eco32I GATATC 1 cut(s) 1209
Eco47I GGWCC 1 cut(s) 290
Eco53kI GAGCTC 2 cut(s) 626, 748
Eco88I CYCGRG 2 cut(s) 206, 459
EcoICRI GAGCTC 2 cut(s) 626, 748
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 2 cut(s) 336, 1174
EcoRV GATATC 1 cut(s) 1209
EcoT38I GRGCYC 2 cut(s) 628, 750
FaeI CATG 8 cut(s) 59, 151, 169, 187, 274, 722, 871, 1205
FaqI GGGAC 1 cut(s) 863
FatI CATG 8 cut(s) 55, 147, 165, 183, 270, 718, 867, 1201
FauNDI CATATG 2 cut(s) 584, 1115
FbaI TGATCA 1 cut(s) 883
FokI GGATG 6 cut(s) 30, 79, 386, 428, 717, 1208
FriOI GRGCYC 2 cut(s) 628, 750
FspBI CTAG 1 cut(s) 872
GlaI GCGC 3 cut(s) 109, 124, 471
GsaI CCCAGC 1 cut(s) 197
GsuI CTGGAG 1 cut(s) 420
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 2 cut(s) 179, 992
HapII CCGG 8 cut(s) 30, 120, 159, 176, 207, 247, 267, 647
HgaI GACGC 1 cut(s) 790
HhaI GCGC 3 cut(s) 110, 125, 472
Hin1II CATG 8 cut(s) 59, 151, 169, 187, 274, 722, 871, 1205
Hin6I GCGC 3 cut(s) 108, 123, 470
HinP1I GCGC 3 cut(s) 108, 123, 470
HinfI GANTC 5 cut(s) 162, 281, 527, 714, 851
HpaII CCGG 8 cut(s) 30, 120, 159, 176, 207, 247, 267, 647
HphI GGTGA 3 cut(s) 673, 764, 796
Hpy166II GTNNAC 2 cut(s) 238, 877
Hpy188I TCNGA 4 cut(s) 155, 257, 763, 783
Hpy188III TCNNGA 6 cut(s) 399, 459, 647, 817, 1047, 1268
Hpy8I GTNNAC 2 cut(s) 238, 877
HpyAV CCTTC 2 cut(s) 68, 768
HpyCH4III ACNGT 2 cut(s) 294, 1150
HpyCH4V TGCA 8 cut(s) 313, 412, 593, 615, 656, 944, 949, 976
HpyF10VI GCNNNNNNNGC 4 cut(s) 605, 754, 831, 955
HpyF3I CTNAG 5 cut(s) 70, 223, 533, 635, 1190
Hsp92II CATG 8 cut(s) 59, 151, 169, 187, 274, 722, 871, 1205
HspAI GCGC 3 cut(s) 108, 123, 470
Kpn2I TCCGGA 1 cut(s) 646
KroI GCCGGC 1 cut(s) 266
KroNI GCCGGC 1 cut(s) 268
Ksp22I TGATCA 1 cut(s) 883
Kzo9I GATC 5 cut(s) 150, 795, 883, 932, 1139
LguI GCTCTTC 1 cut(s) 1016
LmnI GCTCC 2 cut(s) 155, 902
LweI GCATC 3 cut(s) 450, 486, 903
MaeI CTAG 1 cut(s) 872
MaeIII GTNAC 5 cut(s) 37, 423, 513, 700, 802
MalI GATC 5 cut(s) 152, 797, 885, 934, 1141
MboI GATC 5 cut(s) 150, 795, 883, 932, 1139
MboII GAAGA 3 cut(s) 41, 78, 1003
MhlI GDGCHC 3 cut(s) 628, 750, 1183
MluCI AATT 4 cut(s) 201, 405, 454, 735
MlyI GAGTC 2 cut(s) 290, 845
MmeI TCCRAC 2 cut(s) 235, 761
MroI TCCGGA 1 cut(s) 646
MroNI GCCGGC 1 cut(s) 266
MseI TTAA 1 cut(s) 986
MslI CAYNNNNRTG 2 cut(s) 188, 723
MspI CCGG 8 cut(s) 30, 120, 159, 176, 207, 247, 267, 647
MspR9I CCNGG 5 cut(s) 176, 207, 208, 338, 1176
MvaI CCWGG 2 cut(s) 338, 1176
MwoI GCNNNNNNNGC 4 cut(s) 605, 754, 831, 955
NaeI GCCGGC 1 cut(s) 268
NciI CCSGG 3 cut(s) 176, 207, 208
NdeI CATATG 2 cut(s) 584, 1115
NdeII GATC 5 cut(s) 150, 795, 883, 932, 1139
NgoMIV GCCGGC 1 cut(s) 266
NlaIII CATG 8 cut(s) 59, 151, 169, 187, 274, 722, 871, 1205
NlaIV GGNNCC 2 cut(s) 157, 898
NmeAIII GCCGAG 1 cut(s) 545
NmuCI GTSAC 3 cut(s) 37, 700, 802
NspI RCATGY 1 cut(s) 871
PaeR7I CTCGAG 1 cut(s) 459
PciSI GCTCTTC 1 cut(s) 1016
PcsI WCGNNNNNNNCGW 1 cut(s) 290
PdiI GCCGGC 1 cut(s) 268
PfeI GAWTC 3 cut(s) 162, 527, 714
PleI GAGTC 2 cut(s) 289, 845
PpsI GAGTC 2 cut(s) 289, 845
Psp124BI GAGCTC 2 cut(s) 628, 750
Psp6I CCWGG 2 cut(s) 336, 1174
PspFI CCCAGC 1 cut(s) 193
PspGI CCWGG 2 cut(s) 336, 1174
PspN4I GGNNCC 2 cut(s) 157, 898
PspPI GGNCC 2 cut(s) 290, 990
RsaI GTAC 3 cut(s) 237, 391, 1121
RsaNI GTAC 3 cut(s) 236, 390, 1120
RseI CAYNNNNRTG 2 cut(s) 188, 723
SacI GAGCTC 2 cut(s) 628, 750
SapI GCTCTTC 1 cut(s) 1016
SaqAI TTAA 1 cut(s) 986
Sau3AI GATC 5 cut(s) 150, 795, 883, 932, 1139
Sau96I GGNCC 2 cut(s) 290, 990
ScaI AGTACT 1 cut(s) 391
SchI GAGTC 2 cut(s) 290, 845
ScrFI CCNGG 5 cut(s) 176, 207, 208, 338, 1176
SduI GDGCHC 3 cut(s) 628, 750, 1183
SfaNI GCATC 3 cut(s) 450, 486, 903
Sfr274I CTCGAG 1 cut(s) 459
SinI GGWCC 1 cut(s) 290
SlaI CTCGAG 1 cut(s) 459
SmaI CCCGGG 1 cut(s) 208
SmiMI CAYNNNNRTG 2 cut(s) 188, 723
SmlI CTYRAG 2 cut(s) 459, 1045
SmoI CTYRAG 2 cut(s) 459, 1045
Sse9I AATT 4 cut(s) 201, 405, 454, 735
SsiI CCGC 1 cut(s) 1034
SspI AATATT 2 cut(s) 669, 1239
SspMI CTAG 1 cut(s) 872
SstI GAGCTC 2 cut(s) 628, 750
StyD4I CCNGG 5 cut(s) 174, 205, 206, 336, 1174
TaaI ACNGT 2 cut(s) 294, 1150
TaqI TCGA 4 cut(s) 302, 460, 818, 931
TasI AATT 4 cut(s) 201, 405, 454, 735
TatI WGTACW 2 cut(s) 235, 389
TfiI GAWTC 3 cut(s) 162, 527, 714
Tru1I TTAA 1 cut(s) 986
Tru9I TTAA 1 cut(s) 986
TscAI CASTG 2 cut(s) 573, 1153
TseFI GTSAC 3 cut(s) 37, 700, 802
Tsp45I GTSAC 3 cut(s) 37, 700, 802
TspMI CCCGGG 1 cut(s) 206
TspRI CASTG 2 cut(s) 573, 1153
VpaK11BI GGWCC 1 cut(s) 290
XapI RAATTY 2 cut(s) 405, 454
XceI RCATGY 1 cut(s) 871
XcmI CCANNNNNNNNNTGG 1 cut(s) 190
XhoI CTCGAG 1 cut(s) 459
XmaI CCCGGG 1 cut(s) 206
XspI CTAG 1 cut(s) 872
ZrmI AGTACT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.