pycom01g17700

Amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004145
Physical Location & Seq
Forward (+)
16879319 .. 16881374
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g17700.2

Sequence Viewer

Length: 1344 bp
ATGGTGAAACAACAAGACACCAATGGAGGAGCTCAAGTCCTTGCCCTTCCACTCTTACAGGACCCCAAGTCCTTGGAAGCTCAAACTTCGTCACAACAAGGGGCCTCATTTTCCGGGGCTGTCTTCAACATATCGACCACAATGATTGGTGCCGGTATCATGTCGATTCCGGCAACCACCAAGGTCCTCGGCGTAATTCCTGGCTTGCTTCTGATACTGCTGGTCGCTTTCTTTGTGGAAGTCACGGTGGAGTTCCTGCTCAGGTACACCAACTCCGGTGAGTCTGAAACGTACGCCGGCATGGTGGGGGAGTCTTTTGGGGCGTGGGGTTCGGTGGCTGTGCAGATTTGTGTCATTGTCACCAACCTTGGTTGCCTCATCATTTATTTCATCATCATCGGGGATGTGCTGTGTGGGAGTCAGTCTCGAGGAACGTTGCATTTGGGCATTCTGCAAGAATGGTTCGGCATCCACTGGTGGAATTCTCGCGCTTGGGCACTGCTCTTCGTTGCAGTCTTCGTGATGCTTCCGTTAGTCTTGTTACGCCGAGTAGATTCACTAAGGCACACTTCGGCAGTATCCATTCTGCTAGCAGTGCTTTTTGTTGTCATATGTTCTGCAATGGCAATTTACGCGTTGTGCAAAGGGAAGACTCAGAAACCGAGGTTGTTTCCAGACTTCGCAAATCAAGTCTCGGTGTTTGATCTTTTCACTACCATCCCAGTTCTTGTGACAGGTTTCGGATTCCATGTCAACGTTCATCCAATTCGAGCAGAGCTCGGCAAACCATCTGATATGAGATCAGCTACTCGGATTGCTCTCCTGATCAGTGTTGTCATTTACTTTTCTATTGGATTCTTCGGATACCTACTCTTTGGTGACTCAATAATGGACGATATTCTAGTAAACTTCGATCAAACTTCTGAGTCCACATCCGGACAGATAATCAATGACGTTGTTCGATTAAGCTATGCAGTTCACCTCCTTTTCGTGTTTCCTATCATGAACTTTTCCTTGAGGGCCAACATAGATGAATTGCTCTTCCGAAATAGGCCTGTTTTAGCCCAAGACACCTCAAGATTTCTGCCCCTCACTCTTTTCCTGCTCTCCTTCACATACATTGTGGCAATAGCCATCCCAAACATTTGGTATTTCTTTCAGTTCATGGGATCCACCACCGTTGTTTTCCTCTCGTTTATATTCCCGGCATGTCTTATCCTCAGGGATGGACACGGTATATCAACGAGGAGGGACAAGATCATCGCGATTTTCGTGATTATTTTGGCCGTGGCGACAAGCTCAATTGCAATATCCACCACTTTGTATAATTCTGCTAAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

448

Amino Acids

49.06

Weight (kDa)

7.65

Isoelectric Point (pI)

32.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 149
AccII CGCG 3 cut(s) 489, 635, 1265
AccIII TCCGGA 1 cut(s) 935
AclI AACGTT 2 cut(s) 434, 756
AclWI GGATC 2 cut(s) 1164, 1177
AcoI YGGCCR 1 cut(s) 1284
AcsI RAATTY 1 cut(s) 481
AfaI GTAC 2 cut(s) 266, 293
AflIII ACRYGT 1 cut(s) 633
AgsI TTSAA 1 cut(s) 127
AhdI GACNNNNNGTC 1 cut(s) 67
AjnI CCWGG 1 cut(s) 199
AluBI AGCT 6 cut(s) 32, 80, 778, 806, 969, 1299
AluI AGCT 6 cut(s) 32, 80, 778, 806, 969, 1299
Alw21I GWGCWC 2 cut(s) 34, 780
Alw26I GTCTC 2 cut(s) 429, 697
AlwI GGATC 2 cut(s) 1164, 1177
Ama87I CYCGRG 1 cut(s) 426
Aor13HI TCCGGA 1 cut(s) 935
AoxI GGCC 4 cut(s) 102, 1020, 1052, 1284
ApoI RAATTY 1 cut(s) 481
AspLEI GCGC 1 cut(s) 491
AspS9I GGNCC 4 cut(s) 61, 102, 184, 1020
AsuC2I CCSGG 2 cut(s) 115, 1205
AsuHPI GGTGA 5 cut(s) 16, 290, 352, 890, 971
AsuNHI GCTAGC 1 cut(s) 589
AvaI CYCGRG 1 cut(s) 426
AvaII GGWCC 2 cut(s) 61, 184
AxyI CCTNAGG 1 cut(s) 1220
BaeGI GKGCMC 1 cut(s) 499
BamHI GGATCC 1 cut(s) 1169
BanI GGYRCC 1 cut(s) 149
BanII GRGCYC 2 cut(s) 34, 780
BbsI GAAGAC 3 cut(s) 115, 508, 656
Bbv12I GWGCWC 2 cut(s) 34, 780
BccI CCATC 4 cut(s) 725, 796, 1142, 1220
BceAI ACGGC 1 cut(s) 1271
BciT130I CCWGG 1 cut(s) 201
BciVI GTATCC 2 cut(s) 589, 857
BclI TGATCA 1 cut(s) 825
BcnI CCSGG 2 cut(s) 115, 1205
BcoDI GTCTC 2 cut(s) 429, 697
BfaI CTAG 2 cut(s) 590, 902
BfuI GTATCC 2 cut(s) 589, 857
Bme1390I CCNGG 3 cut(s) 115, 201, 1205
Bme18I GGWCC 2 cut(s) 61, 184
BmeRI GACNNNNNGTC 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 426
BmgT120I GGNCC 4 cut(s) 61, 102, 184, 1020
BmiI GGNNCC 4 cut(s) 63, 103, 151, 1171
BmrFI CCNGG 3 cut(s) 115, 201, 1205
BmrI ACTGGG 1 cut(s) 716
BmsI GCATC 2 cut(s) 477, 513
BmtI GCTAGC 1 cut(s) 593
BmuI ACTGGG 1 cut(s) 716
BpiI GAAGAC 3 cut(s) 115, 508, 656
BplI GAGNNNNNCTC 4 cut(s) 409, 441, 762, 794
Bpu10I CCTNAGC 1 cut(s) 260
BpuEI CTTGAG 3 cut(s) 18, 1036, 1060
BpuMI CCSGG 2 cut(s) 115, 1205
BsaJI CCNNGG 7 cut(s) 72, 114, 180, 187, 367, 662, 1287
BsaWI WCCGGW 2 cut(s) 275, 935
BsaXI ACNNNNNCTCC 4 cut(s) 21, 51, 257, 287
Bse118I RCCGGY 2 cut(s) 152, 296
Bse1I ACTGG 2 cut(s) 479, 722
Bse21I CCTNAGG 1 cut(s) 1220
Bse3DI GCAATG 1 cut(s) 627
BseAI TCCGGA 1 cut(s) 935
BseBI CCWGG 1 cut(s) 201
BseDI CCNNGG 7 cut(s) 72, 114, 180, 187, 367, 662, 1287
BseGI GGATG 7 cut(s) 409, 468, 717, 760, 932, 1134, 1231
BseMI GCAATG 1 cut(s) 627
BseMII CTCAG 4 cut(s) 274, 668, 915, 1234
BseNI ACTGG 2 cut(s) 479, 722
BseRI GAGGAG 2 cut(s) 42, 1261
BseSI GKGCMC 1 cut(s) 499
BsgI GTGCAG 1 cut(s) 362
Bsh1236I CGCG 3 cut(s) 489, 635, 1265
BshFI GGCC 4 cut(s) 104, 1022, 1054, 1286
BshNI GGYRCC 1 cut(s) 149
BsiHKAI GWGCWC 2 cut(s) 34, 780
BsiHKCI CYCGRG 1 cut(s) 426
BsiSI CCGG 7 cut(s) 114, 153, 170, 276, 297, 936, 1205
BsiWI CGTACG 1 cut(s) 291
BslFI GGGAC 1 cut(s) 1265
BsmAI GTCTC 2 cut(s) 429, 697
BsmFI GGGAC 1 cut(s) 1265
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 4 cut(s) 104, 1022, 1054, 1286
BsoBI CYCGRG 1 cut(s) 426
Bsp1286I GDGCHC 3 cut(s) 34, 499, 780
Bsp13I TCCGGA 1 cut(s) 935
Bsp143I GATC 6 cut(s) 703, 800, 825, 913, 1169, 1257
Bsp68I TCGCGA 1 cut(s) 1265
BspANI GGCC 4 cut(s) 104, 1022, 1054, 1286
BspCNI CTCAG 4 cut(s) 273, 667, 916, 1233
BspEI TCCGGA 1 cut(s) 935
BspFNI CGCG 3 cut(s) 489, 635, 1265
BspHI TCATGA 1 cut(s) 1002
BspLI GGNNCC 4 cut(s) 63, 103, 151, 1171
BspOI GCTAGC 1 cut(s) 593
BspPI GGATC 2 cut(s) 1164, 1177
BspQI GCTCTTC 2 cut(s) 509, 1046
BspT107I GGYRCC 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 627
BsrFI RCCGGY 2 cut(s) 152, 296
BsrI ACTGG 2 cut(s) 479, 722
BssAI RCCGGY 2 cut(s) 152, 296
BssECI CCNNGG 7 cut(s) 72, 114, 180, 187, 367, 662, 1287
BssMI GATC 6 cut(s) 703, 800, 825, 913, 1169, 1257
BssT1I CCWWGG 3 cut(s) 72, 180, 367
Bst2UI CCWGG 1 cut(s) 201
Bst4CI ACNGT 3 cut(s) 247, 1180, 1235
Bst6I CTCTTC 2 cut(s) 509, 1046
BstC8I GCNNGC 3 cut(s) 206, 298, 591
BstDEI CTNAG 5 cut(s) 260, 560, 654, 924, 1220
BstDSI CCRYGG 1 cut(s) 1287
BstF5I GGATG 7 cut(s) 409, 468, 717, 760, 932, 1134, 1231
BstFNI CGCG 3 cut(s) 489, 635, 1265
BstHHI GCGC 1 cut(s) 491
BstKTI GATC 6 cut(s) 706, 803, 828, 916, 1172, 1260
BstMAI GTCTC 2 cut(s) 429, 697
BstMBI GATC 6 cut(s) 703, 800, 825, 913, 1169, 1257
BstMWI GCNNNNNNNGC 2 cut(s) 595, 632
BstNI CCWGG 1 cut(s) 201
BstNSI RCATGY 1 cut(s) 1212
BstSCI CCNGG 3 cut(s) 113, 199, 1203
BstSLI GKGCMC 1 cut(s) 499
BstUI CGCG 3 cut(s) 489, 635, 1265
BstV2I GAAGAC 3 cut(s) 115, 508, 656
BstX2I RGATCY 1 cut(s) 1169
BstXI CCANNNNNNTGG 2 cut(s) 73, 1146
BstYI RGATCY 1 cut(s) 1169
Bsu36I CCTNAGG 1 cut(s) 1220
BsuI GTATCC 2 cut(s) 589, 857
BsuRI GGCC 4 cut(s) 104, 1022, 1054, 1286
BtgI CCRYGG 1 cut(s) 1287
BtgZI GCGATG 1 cut(s) 1246
BtsCI GGATG 7 cut(s) 409, 468, 717, 760, 932, 1134, 1231
BtsI GCAGTG 2 cut(s) 497, 600
BtsIMutI CAGTG 4 cut(s) 472, 497, 600, 835
BtuMI TCGCGA 1 cut(s) 1265
Cac8I GCNNGC 3 cut(s) 206, 298, 591
CciI TCATGA 1 cut(s) 1002
CfoI GCGC 1 cut(s) 491
Cfr10I RCCGGY 2 cut(s) 152, 296
Cfr13I GGNCC 4 cut(s) 61, 102, 184, 1020
Csp6I GTAC 2 cut(s) 265, 292
CspCI CAANNNNNGTGG 4 cut(s) 127, 162, 1162, 1197
CviAII CATG 6 cut(s) 160, 301, 749, 1003, 1165, 1209
CviQI GTAC 2 cut(s) 265, 292
DdeI CTNAG 5 cut(s) 260, 560, 654, 924, 1220
DpnI GATC 6 cut(s) 705, 802, 827, 915, 1171, 1259
DpnII GATC 6 cut(s) 703, 800, 825, 913, 1169, 1257
DriI GACNNNNNGTC 1 cut(s) 67
EaeI YGGCCR 1 cut(s) 1284
Eam1104I CTCTTC 2 cut(s) 509, 1046
Eam1105I GACNNNNNGTC 1 cut(s) 67
EarI CTCTTC 2 cut(s) 509, 1046
Ecl136II GAGCTC 2 cut(s) 32, 778
Eco130I CCWWGG 3 cut(s) 72, 180, 367
Eco147I AGGCCT 1 cut(s) 1054
Eco24I GRGCYC 2 cut(s) 34, 780
Eco47I GGWCC 2 cut(s) 61, 184
Eco53kI GAGCTC 2 cut(s) 32, 778
Eco81I CCTNAGG 1 cut(s) 1220
Eco88I CYCGRG 1 cut(s) 426
EcoICRI GAGCTC 2 cut(s) 32, 778
EcoO109I RGGNCCY 3 cut(s) 61, 102, 184
EcoRI GAATTC 1 cut(s) 481
EcoRII CCWGG 1 cut(s) 199
EcoT14I CCWWGG 3 cut(s) 72, 180, 367
EcoT38I GRGCYC 2 cut(s) 34, 780
ErhI CCWWGG 3 cut(s) 72, 180, 367
FaeI CATG 6 cut(s) 163, 304, 752, 1006, 1168, 1212
FalI AAGNNNNNCTT 2 cut(s) 553, 585
FaqI GGGAC 1 cut(s) 1265
FatI CATG 6 cut(s) 159, 300, 748, 1002, 1164, 1208
FauNDI CATATG 1 cut(s) 611
FbaI TGATCA 1 cut(s) 825
FokI GGATG 7 cut(s) 416, 455, 704, 747, 919, 1121, 1238
FriOI GRGCYC 2 cut(s) 34, 780
FspBI CTAG 2 cut(s) 590, 902
GlaI GCGC 1 cut(s) 490
HaeIII GGCC 4 cut(s) 104, 1022, 1054, 1286
HapII CCGG 7 cut(s) 114, 153, 170, 276, 297, 936, 1205
HhaI GCGC 1 cut(s) 491
Hin1II CATG 6 cut(s) 163, 304, 752, 1006, 1168, 1212
Hin6I GCGC 1 cut(s) 489
HinP1I GCGC 1 cut(s) 489
HincII GTYRAC 1 cut(s) 754
HindII GTYRAC 1 cut(s) 754
HpaII CCGG 7 cut(s) 114, 153, 170, 276, 297, 936, 1205
HphI GGTGA 5 cut(s) 16, 290, 352, 890, 971
Hpy166II GTNNAC 5 cut(s) 267, 754, 907, 930, 979
Hpy188I TCNGA 9 cut(s) 213, 286, 657, 743, 793, 813, 863, 925, 1046
Hpy188III TCNNGA 9 cut(s) 426, 520, 674, 823, 936, 1003, 1077, 1264, 1273
Hpy8I GTNNAC 5 cut(s) 267, 754, 907, 930, 979
HpyAV CCTTC 2 cut(s) 56, 1120
HpyCH4III ACNGT 3 cut(s) 247, 1180, 1235
HpyCH4IV ACGT 4 cut(s) 290, 434, 756, 954
HpyCH4V TGCA 8 cut(s) 343, 439, 454, 512, 620, 642, 974, 1307
HpyF10VI GCNNNNNNNGC 2 cut(s) 595, 632
HpyF3I CTNAG 5 cut(s) 260, 560, 654, 924, 1220
HpySE526I ACGT 4 cut(s) 290, 434, 756, 954
Hsp92II CATG 6 cut(s) 163, 304, 752, 1006, 1168, 1212
HspAI GCGC 1 cut(s) 489
Kpn2I TCCGGA 1 cut(s) 935
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
Ksp22I TGATCA 1 cut(s) 825
Kzo9I GATC 6 cut(s) 703, 800, 825, 913, 1169, 1257
LguI GCTCTTC 2 cut(s) 509, 1046
LmnI GCTCC 1 cut(s) 29
LweI GCATC 2 cut(s) 477, 513
MaeI CTAG 2 cut(s) 590, 902
MaeII ACGT 4 cut(s) 290, 434, 756, 954
MaeIII GTNAC 6 cut(s) 90, 241, 358, 540, 730, 878
MalI GATC 6 cut(s) 705, 802, 827, 915, 1171, 1259
MboI GATC 6 cut(s) 703, 800, 825, 913, 1169, 1257
MboII GAAGA 6 cut(s) 115, 496, 508, 661, 850, 1033
MfeI CAATTG 1 cut(s) 1302
MflI RGATCY 1 cut(s) 1169
MhlI GDGCHC 3 cut(s) 34, 499, 780
MluCI AATT 7 cut(s) 195, 481, 627, 765, 1034, 1302, 1327
MluI ACGCGT 1 cut(s) 633
MlyI GAGTC 6 cut(s) 290, 320, 427, 646, 875, 935
MroI TCCGGA 1 cut(s) 935
MroNI GCCGGC 1 cut(s) 296
MseI TTAA 1 cut(s) 965
MspI CCGG 7 cut(s) 114, 153, 170, 276, 297, 936, 1205
MspR9I CCNGG 3 cut(s) 115, 201, 1205
MunI CAATTG 1 cut(s) 1302
Mva1269I GAATGC 1 cut(s) 447
MvaI CCWGG 1 cut(s) 201
MvnI CGCG 3 cut(s) 489, 635, 1265
MwoI GCNNNNNNNGC 2 cut(s) 595, 632
NaeI GCCGGC 1 cut(s) 298
NciI CCSGG 2 cut(s) 115, 1205
NdeI CATATG 1 cut(s) 611
NdeII GATC 6 cut(s) 703, 800, 825, 913, 1169, 1257
NgoMIV GCCGGC 1 cut(s) 296
NheI GCTAGC 1 cut(s) 589
NlaIII CATG 6 cut(s) 163, 304, 752, 1006, 1168, 1212
NlaIV GGNNCC 4 cut(s) 63, 103, 151, 1171
NmeAIII GCCGAG 3 cut(s) 168, 572, 759
NmuCI GTSAC 5 cut(s) 90, 241, 358, 730, 878
NruI TCGCGA 1 cut(s) 1265
NspI RCATGY 1 cut(s) 1212
PaeR7I CTCGAG 1 cut(s) 426
PagI TCATGA 1 cut(s) 1002
PceI AGGCCT 1 cut(s) 1054
PciSI GCTCTTC 2 cut(s) 509, 1046
PcsI WCGNNNNNNNCGW 1 cut(s) 1269
PctI GAATGC 1 cut(s) 447
PdiI GCCGGC 1 cut(s) 298
PfeI GAWTC 4 cut(s) 166, 554, 744, 855
Pfl23II CGTACG 1 cut(s) 291
PleI GAGTC 6 cut(s) 289, 319, 426, 646, 875, 934
PpsI GAGTC 6 cut(s) 289, 319, 426, 646, 875, 934
PpuMI RGGWCCY 2 cut(s) 61, 184
Psp124BI GAGCTC 2 cut(s) 34, 780
Psp1406I AACGTT 2 cut(s) 434, 756
Psp5II RGGWCCY 2 cut(s) 61, 184
Psp6I CCWGG 1 cut(s) 199
PspGI CCWGG 1 cut(s) 199
PspLI CGTACG 1 cut(s) 291
PspN4I GGNNCC 4 cut(s) 63, 103, 151, 1171
PspPI GGNCC 4 cut(s) 61, 102, 184, 1020
PspPPI RGGWCCY 2 cut(s) 61, 184
PsuI RGATCY 1 cut(s) 1169
RruI TCGCGA 1 cut(s) 1265
RsaI GTAC 2 cut(s) 266, 293
RsaNI GTAC 2 cut(s) 265, 292
SacI GAGCTC 2 cut(s) 34, 780
SapI GCTCTTC 2 cut(s) 509, 1046
SaqAI TTAA 1 cut(s) 965
Sau3AI GATC 6 cut(s) 703, 800, 825, 913, 1169, 1257
Sau96I GGNCC 4 cut(s) 61, 102, 184, 1020
SchI GAGTC 6 cut(s) 290, 320, 427, 646, 875, 935
ScrFI CCNGG 3 cut(s) 115, 201, 1205
SduI GDGCHC 3 cut(s) 34, 499, 780
SfaNI GCATC 2 cut(s) 477, 513
Sfr274I CTCGAG 1 cut(s) 426
SinI GGWCC 2 cut(s) 61, 184
SlaI CTCGAG 1 cut(s) 426
SmlI CTYRAG 4 cut(s) 33, 426, 1015, 1075
SmoI CTYRAG 4 cut(s) 33, 426, 1015, 1075
Sse9I AATT 7 cut(s) 195, 481, 627, 765, 1034, 1302, 1327
SseBI AGGCCT 1 cut(s) 1054
SspMI CTAG 2 cut(s) 590, 902
SstI GAGCTC 2 cut(s) 34, 780
StuI AGGCCT 1 cut(s) 1054
StyD4I CCNGG 3 cut(s) 113, 199, 1203
StyI CCWWGG 3 cut(s) 72, 180, 367
TaaI ACNGT 3 cut(s) 247, 1180, 1235
TaiI ACGT 4 cut(s) 293, 437, 759, 957
TaqI TCGA 6 cut(s) 134, 164, 427, 769, 912, 961
TasI AATT 7 cut(s) 195, 481, 627, 765, 1034, 1302, 1327
TfiI GAWTC 4 cut(s) 166, 554, 744, 855
Tru1I TTAA 1 cut(s) 965
Tru9I TTAA 1 cut(s) 965
TscAI CASTG 4 cut(s) 479, 504, 600, 835
TseFI GTSAC 5 cut(s) 90, 241, 358, 730, 878
Tsp45I GTSAC 5 cut(s) 90, 241, 358, 730, 878
TspDTI ATGAA 5 cut(s) 379, 749, 1019, 1047, 1153
TspGWI ACGGA 1 cut(s) 519
TspRI CASTG 4 cut(s) 479, 504, 600, 835
VpaK11BI GGWCC 2 cut(s) 61, 184
XapI RAATTY 1 cut(s) 481
XceI RCATGY 1 cut(s) 1212
XhoI CTCGAG 1 cut(s) 426
XspI CTAG 2 cut(s) 590, 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.