FvH4_7g27291

Structural maintenance of chromosomes protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003625
Physical Location & Seq
Forward (+)
20432669 .. 20443868
11200 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27291.t8

Sequence Viewer

Length: 3159 bp
ATGGCCGAGCCAATCGCCGGCCGCCGCAACAGTTCCAAAGCCGGAATCGTCCAGAAAATCCGGCTGGAGAATTTCATGTGCCACACTAGCTTGCAGATCGAGCTCGGCGATTGGGTCAATTTCATCACCGGTCAAAATGGAAGTGGTAAAAGTGCAATACTGACGGCTCTGTGTGTTGCATTTGGGTCTCGAGCTAAGGAGACTCAGAGAGGGTCTACATTGAAGGATTTTATAAAGACTGGATGCAGTTACGCTGTTGTTCATGTCGAATTGAAGAACCAAGGGGAGGATGCTTTTAAGCATGATATTTATGGGGATGTCATAATCATTGAGCGGAAGATATCTTCTGGAAGTTCAAACGCCACGGTTTTGAAGGATCATCAAGGAAGAAAGGTTGCTAGCCGGAAAGAGGATCTCCGGGAGCTTATTGAGCATTTTAGTATTGATGTTGAGAATCCTTGTGTAATAATGGGTCAAGACAGAAGCAGAGAGTTTTTGCATTCTGGAAATGACAAAGATAAATTTAAGTTCTTTTACAAGGCCACACTTCTGCAGCAAGTTGAAGAGCTCCTGCAAGACATCGAGAAGCAGTTGGAGAAGGCAAATGTTGTTGTTGATCACTTAGAGGGCTCCATAAGACCCATAGAAAGGGAACTTCATGAATTACAGGAAAAGATTAGAAACTTGGAACACGTTGAAGAAATATCCCAACAAGCAAAGCAGCTAAAAAAGAAGCTTGCTTGGTCATGGGTATACGATGTAGACAGGCAACTCCAGCAAAAGAATGTGCATATTGGAAAGCTGAAAGATCGTATACCTCTTTGTCAAGCTAAAATTGATCAGTTTAGAGATGAAGTGGAGAAACTTAACCAATGCTATACCTTGAAGAGATCCGAGATTGCAAGCATGGTGGAAAAGACTTCGGAAGTTAGGAGAATGAAGGAGGAATTGAAACAGACGCTAGCATTGGCTACAAAAGATAAGCTTAAGCTAGAAGAAGAATATGGCCGCAGAACTAACTACATCCAGAAATTGACAAACACTGCTAGGTCTCTTCAGCAACAGATTCAGGATGCCGAGGATCAGCATGCCAGAAGTACACAGGCGGAAGAATCTGCAATGGAGGAAAAGCTGAAAGAACTACAAAATGAGATTGCTACTGTTGAATCAATGCTTACAAGGTTGAAGGAAGAAGATAGTGTCTTATCAGAAAGTGTACGCAAGACAAGTAGTACAATTGGTGAATTAAGTCAAACGATTCAAAGAAAAGAGAAGGAGTGTCTAGACATCTCAAATCACATCCGTAAGCTTGAGAGAAATCATGCTAACAAGGTCACAGCTTTTGGTGGAGATAAAGTGATAAGCCTTTTACGTACAATTGAGAGGTATCATAAGAGATTCAAATGCCCTCCAATTGGTCCTATCGGTGCTCATTTGACTTTGAATGATGGAGATGTATGGGCAACAACAATTGAAATTGCTATAGGCAGGTTGCTCAATGCTTTCATTGTAACAGATCATAAGGACTCTCAACTTCTCAGAACATGTGCAAGGGAAGCCAACTACAACCACCTTCAGATTATCATATATGACTTTTCATTACCAAGGTTGAATATTCCACCTCACATGCTTCCACAAACAGAGCACCCAACTACTCTTTCTCTTCTCCATTCTGAAAATCATACCGTGTTAAATGTCCTGGTAGATTTGGGAAGTGTTGAAAGGCAAGTCCTTGTTGAAAATTATGAGGAGGGAAAGGAAGTTGCTTTTGACCACAGAATTCCCAATCTGAAGGAAGTCTTCACAAAAGACTTGAAGAAGATGTTTTCGCGTAATGGTGTCCAGACTACTCTTCCTCTCAATAGGCAAGACCGACCTGCTCGTCTCTGTGCTAATTATGACGTTGAAATCAATAATTGTATTAGAGAAGCATCAGGTGCACAAGAAGAAGCTCAGCGGTGCAGGAGGAGAAAAAGGGATGAAGAAGACAAACTTCGGGATCTTAACGAGGAACTCCAGAGTGTGAAGAGGAGGCGCATGAATGCTGAGCATGATTTGGCGTCTAAGAAACTAGCTATTCAAGACAGTGTATATGATGCGGAAGCCAATACATCACTTGTATCAACTGTAGATGAGCTTCATAGAGACGTTTCAAAAGTCCAAGAGGAGATTCAAGAGAAGGAAATGTTGCTTGGAAACTTTCGAGTCAGAATTAATGAAGCTGAGGCGAAGACCAGTGATCTTAAAGTAACATTTGATAATCTAACTGAGTCAGCAAGAGGAGACATTGAGGCCATTGAGAAGGCAGAGAGAGATTTGATGGAGATAGACATAAATCTAGCTTCTGCAGAAGCGGAGAAGCTTCGTTTTGAAAGTGCAATGAAGACCAAGATCCTTCCTGCAATTAATGAAGCTGAAAAACAATATAAAGAGCTTGAGCATCAGCGAGAGGAAAACTGTAGAAAAGCTTCAATCCTTTGTCCTGAAAGTGAAATTATAGCGTTGGGTGACTGGGATGGTAGCACTCCAGAACAACTCAGTACTCAATTAACCAGGCTGAATCAGAAACTTCAACGAGAGAGTGAGAGATGCACTGAATCCATTGATGAGCTTCGGATGTCATATGAGAGTAAAGAACGTAAGATTTTAAGAAAACAGAAAATATACAGAGCTTTCCGGGAAAAGTTGAATGCTTGCCAGAAAGCCCTAAATATGCGAAGTGAAAAGTTTGAGAGAAATAAAACTTTACTAAAGCGCCAGATGACGTGGCTATTTAATTCTCATCTGGGAAGGAAAGGGTTCAGTGGAAAGATTAAGGTTAGCTATGAGGAGAGAACCCTTTCAATTGAGGTAAAGATGCCCCAGGATGCATCAAGCAGCACTGTTCGCGACACCAGAGGGCTTTCAGGTGGGGAACGCTCCTTTTCAACATTGTGCTTTGCGCTGGCTCTTCATGATATGACTGAAGCCCCATTTAGAGCTATGGATGAGTTTGATGTATTTATGGATGCCGTAAGTCGGAAAATCAGCCTTGATACTCTTGTCGATTTTGCATTAGCACAAGGATCCCAATGGGTACTCATCACCCCTCATGATATCAGTATGGTGAAGAATGGGGATCGGATAAAGAAGCAGCAAATGGCGGCTCCTCGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_23 SMC_N

Protein Analysis

1053

Amino Acids

120.91

Weight (kDa)

7.01

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_N PF02463 590 - 919 4.2e-06 RecF/RecN/SMC N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 233
AasI GACNNNNNNGTC 1 cut(s) 1881
Acc36I ACCTGC 2 cut(s) 1479, 1885
AccBSI CCGCTC 1 cut(s) 334
AccI GTMKAC 4 cut(s) 215, 753, 762, 814
AccII CGCG 2 cut(s) 1831, 2889
AciI CCGC 9 cut(s) 22, 25, 334, 1009, 1106, 1957, 2099, 2354, 3143
AclWI GGATC 9 cut(s) 384, 420, 885, 1089, 2007, 2386, 3060, 3073, 3126
AcoI YGGCCR 3 cut(s) 3, 19, 1006
AcsI RAATTY 3 cut(s) 70, 521, 1779
AcuI CTGAAG 4 cut(s) 1040, 1559, 1811, 2985
AcyI GRCGYC 1 cut(s) 2060
AfaI GTAC 6 cut(s) 1099, 1218, 1234, 1375, 2542, 3078
AfiI CCNNNNNNNGG 6 cut(s) 17, 286, 409, 648, 1415, 3018
AflII CTTAAG 1 cut(s) 986
AflIII ACRYGT 2 cut(s) 691, 1544
AgeI ACCGGT 1 cut(s) 128
AjiI CACGTC 1 cut(s) 2766
AjnI CCWGG 3 cut(s) 1698, 2552, 2862
AjuI GAANNNNNNNTTGG 6 cut(s) 1642, 1674, 2154, 2175, 2186, 2207
Alw21I GWGCWC 5 cut(s) 105, 570, 1432, 1647, 1942
Alw26I GTCTC 6 cut(s) 192, 194, 1056, 1889, 2139, 2277
Alw44I GTGCAC 1 cut(s) 1938
AlwI GGATC 9 cut(s) 384, 420, 885, 1089, 2007, 2386, 3060, 3073, 3126
Ama87I CYCGRG 1 cut(s) 189
AoxI GGCC 5 cut(s) 3, 19, 540, 1006, 2292
ApaLI GTGCAC 1 cut(s) 1938
ApeKI GCWGC 4 cut(s) 553, 721, 2877, 3133
ApoI RAATTY 3 cut(s) 70, 521, 1779
AseI ATTAAT 2 cut(s) 2214, 2406
AsiGI ACCGGT 1 cut(s) 128
Asp700I GAANNNNTTC 3 cut(s) 2467, 2798, 2839
AspLEI GCGC 3 cut(s) 2037, 2757, 2944
AspS9I GGNCC 1 cut(s) 1418
AsuC2I CCSGG 2 cut(s) 419, 2678
AsuHPI GGTGA 5 cut(s) 118, 1253, 2519, 3076, 3118
AsuNHI GCTAGC 2 cut(s) 398, 961
AvaI CYCGRG 1 cut(s) 189
AvaII GGWCC 1 cut(s) 1418
BaeGI GKGCMC 1 cut(s) 1942
BaeI ACNNNNGTAYC 2 cut(s) 3027, 3060
BamHI GGATCC 1 cut(s) 3065
BanII GRGCYC 3 cut(s) 105, 570, 632
BarI GAAGNNNNNNTAC 4 cut(s) 2121, 2153, 2452, 2484
BbsI GAAGAC 4 cut(s) 1792, 1992, 2237, 2390
Bbv12I GWGCWC 5 cut(s) 105, 570, 1432, 1647, 1942
BbvCI CCTCAGC 1 cut(s) 2223
BbvI GCAGC 4 cut(s) 565, 733, 2889, 3145
BccI CCATC 3 cut(s) 1442, 2314, 2510
BceAI ACGGC 2 cut(s) 180, 2996
BciT130I CCWGG 3 cut(s) 1700, 2554, 2864
BclI TGATCA 2 cut(s) 616, 838
BcnI CCSGG 2 cut(s) 419, 2678
BcoDI GTCTC 6 cut(s) 192, 194, 1056, 1889, 2139, 2277
BfaI CTAG 8 cut(s) 87, 399, 962, 992, 1047, 1283, 2072, 2339
BfmI CTRYAG 5 cut(s) 551, 1482, 2127, 2346, 2458
BfoI RGCGCY 1 cut(s) 2758
BfrI CTTAAG 1 cut(s) 986
BfuAI ACCTGC 2 cut(s) 1479, 1885
BisI GCNGC 8 cut(s) 22, 25, 554, 722, 1009, 2878, 3134, 3144
BlpI GCTNAGC 2 cut(s) 1953, 2046
BlsI GCNGC 8 cut(s) 23, 26, 555, 723, 1010, 2879, 3135, 3145
BmcAI AGTACT 1 cut(s) 2542
Bme1390I CCNGG 5 cut(s) 419, 1700, 2554, 2678, 2864
Bme18I GGWCC 1 cut(s) 1418
BmeT110I CYCGRG 1 cut(s) 189
BmgBI CACGTC 1 cut(s) 2766
BmgT120I GGNCC 1 cut(s) 1418
BmiI GGNNCC 3 cut(s) 631, 3067, 3147
BmrFI CCNGG 5 cut(s) 419, 1700, 2554, 2678, 2864
BmrI ACTGGG 1 cut(s) 2521
BmtI GCTAGC 2 cut(s) 402, 965
BmuI ACTGGG 1 cut(s) 2521
BpiI GAAGAC 4 cut(s) 1792, 1992, 2237, 2390
BpmI CTGGAG 4 cut(s) 86, 758, 2000, 2511
Bpu10I CCTNAGC 2 cut(s) 195, 2223
Bpu1102I GCTNAGC 2 cut(s) 1953, 2046
BpuEI CTTGAG 2 cut(s) 1331, 2456
BpuMI CCSGG 2 cut(s) 419, 2678
BsaAI YACGTR 1 cut(s) 1373
BsaHI GRCGYC 1 cut(s) 2060
BsaI GGTCTC 2 cut(s) 192, 1056
BsaJI CCNNGG 5 cut(s) 280, 363, 1077, 1604, 2862
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 2 cut(s) 1341, 1371
Bsc4I CCNNNNNNNGG 6 cut(s) 17, 286, 409, 648, 1415, 3018
Bse118I RCCGGY 2 cut(s) 17, 128
Bse1I ACTGG 3 cut(s) 244, 2235, 2516
Bse3DI GCAATG 2 cut(s) 1125, 2385
BseBI CCWGG 3 cut(s) 1700, 2554, 2864
BseDI CCNNGG 5 cut(s) 280, 363, 1077, 1604, 2862
BseLI CCNNNNNNNGG 6 cut(s) 17, 286, 409, 648, 1415, 3018
BseMI GCAATG 2 cut(s) 1125, 2385
BseMII CTCAG 7 cut(s) 218, 1552, 1967, 2037, 2214, 2259, 2551
BseNI ACTGG 3 cut(s) 244, 2235, 2516
BseRI GAGGAG 7 cut(s) 1763, 1981, 2044, 2180, 2295, 2843, 3138
BseSI GKGCMC 1 cut(s) 1942
BseX3I CGGCCG 1 cut(s) 19
BseXI GCAGC 4 cut(s) 565, 733, 2889, 3145
BsgI GTGCAG 1 cut(s) 1981
Bsh1236I CGCG 2 cut(s) 1831, 2889
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 5 cut(s) 5, 21, 542, 1008, 2294
BshTI ACCGGT 1 cut(s) 128
BsiEI CGRYCG 1 cut(s) 22
BsiHKAI GWGCWC 5 cut(s) 105, 570, 1432, 1647, 1942
BsiHKCI CYCGRG 1 cut(s) 189
BsiSI CCGG 7 cut(s) 18, 42, 61, 129, 403, 418, 2677
BslI CCNNNNNNNGG 6 cut(s) 17, 286, 409, 648, 1415, 3018
BsmAI GTCTC 6 cut(s) 192, 194, 1056, 1889, 2139, 2277
BsmBI CGTCTC 2 cut(s) 1889, 2139
BsmI GAATGC 3 cut(s) 499, 2047, 2695
BsnI GGCC 5 cut(s) 5, 21, 542, 1008, 2294
Bso31I GGTCTC 2 cut(s) 192, 1056
BsoBI CYCGRG 1 cut(s) 189
Bsp1286I GDGCHC 6 cut(s) 105, 570, 632, 1432, 1647, 1942
Bsp1720I GCTNAGC 2 cut(s) 1953, 2046
Bsp68I TCGCGA 1 cut(s) 2889
BspACI CCGC 9 cut(s) 22, 25, 334, 1009, 1106, 1957, 2099, 2354, 3143
BspANI GGCC 5 cut(s) 5, 21, 542, 1008, 2294
BspCNI CTCAG 7 cut(s) 217, 1551, 1966, 2038, 2215, 2260, 2550
BspFNI CGCG 2 cut(s) 1831, 2889
BspHI TCATGA 3 cut(s) 658, 2953, 3091
BspLI GGNNCC 3 cut(s) 631, 3067, 3147
BspMAI CTGCAG 2 cut(s) 555, 2350
BspMI ACCTGC 2 cut(s) 1479, 1885
BspOI GCTAGC 2 cut(s) 402, 965
BspPI GGATC 9 cut(s) 384, 420, 885, 1089, 2007, 2386, 3060, 3073, 3126
BspQI GCTCTTC 2 cut(s) 558, 2955
BspTI CTTAAG 1 cut(s) 986
BspTNI GGTCTC 2 cut(s) 192, 1056
BsrBI CCGCTC 1 cut(s) 334
BsrDI GCAATG 2 cut(s) 1125, 2385
BsrFI RCCGGY 2 cut(s) 17, 128
BsrI ACTGG 3 cut(s) 244, 2235, 2516
BssAI RCCGGY 2 cut(s) 17, 128
BssECI CCNNGG 5 cut(s) 280, 363, 1077, 1604, 2862
BssNAI GTATAC 2 cut(s) 754, 815
BssNI GRCGYC 1 cut(s) 2060
BssT1I CCWWGG 2 cut(s) 280, 1604
Bst1107I GTATAC 2 cut(s) 754, 815
Bst2UI CCWGG 3 cut(s) 1700, 2554, 2864
Bst4CI ACNGT 8 cut(s) 32, 367, 1162, 1687, 2087, 2128, 2459, 2884
Bst6I CTCTTC 7 cut(s) 558, 881, 1059, 1668, 1857, 2021, 2955
BstACI GRCGYC 1 cut(s) 2060
BstAFI CTTAAG 1 cut(s) 986
BstAPI GCANNNNNTGC 1 cut(s) 1937
BstBAI YACGTR 1 cut(s) 1373
BstC8I GCNNGC 9 cut(s) 19, 92, 400, 738, 904, 963, 1089, 2695, 2946
BstDSI CCRYGG 1 cut(s) 363
BstFNI CGCG 2 cut(s) 1831, 2889
BstH2I RGCGCY 1 cut(s) 2758
BstHHI GCGC 3 cut(s) 2037, 2757, 2944
BstMAI GTCTC 6 cut(s) 192, 194, 1056, 1889, 2139, 2277
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 7 cut(s) 87, 100, 430, 775, 1556, 1937, 2886
BstNI CCWGG 3 cut(s) 1700, 2554, 2864
BstNSI RCATGY 3 cut(s) 1091, 1548, 1630
BstSCI CCNGG 5 cut(s) 417, 1698, 2552, 2676, 2862
BstSFI CTRYAG 5 cut(s) 551, 1482, 2127, 2346, 2458
BstSLI GKGCMC 1 cut(s) 1942
BstSNI TACGTA 1 cut(s) 1373
BstUI CGCG 2 cut(s) 1831, 2889
BstV1I GCAGC 4 cut(s) 565, 733, 2889, 3145
BstV2I GAAGAC 4 cut(s) 1792, 1992, 2237, 2390
BstX2I RGATCY 5 cut(s) 412, 890, 1999, 2391, 3065
BstYI RGATCY 5 cut(s) 412, 890, 1999, 2391, 3065
BstZ17I GTATAC 2 cut(s) 754, 815
BstZI CGGCCG 1 cut(s) 19
BsuRI GGCC 5 cut(s) 5, 21, 542, 1008, 2294
BtgI CCRYGG 1 cut(s) 363
BtrI CACGTC 1 cut(s) 2766
BtsI GCAGTG 1 cut(s) 1041
BtsIMutI CAGTG 6 cut(s) 1041, 2092, 2242, 2592, 2809, 2880
BtuMI TCGCGA 1 cut(s) 2889
BveI ACCTGC 2 cut(s) 1479, 1885
Cac8I GCNNGC 9 cut(s) 19, 92, 400, 738, 904, 963, 1089, 2695, 2946
CciI TCATGA 3 cut(s) 658, 2953, 3091
CfoI GCGC 3 cut(s) 2037, 2757, 2944
Cfr10I RCCGGY 2 cut(s) 17, 128
Cfr13I GGNCC 1 cut(s) 1418
CseI GACGC 2 cut(s) 967, 2049
Csp6I GTAC 6 cut(s) 1098, 1217, 1233, 1374, 2541, 3077
CspAI ACCGGT 1 cut(s) 128
CspCI CAANNNNNGTGG 2 cut(s) 891, 926
CviQI GTAC 6 cut(s) 1098, 1217, 1233, 1374, 2541, 3077
DrdI GACNNNNNNGTC 1 cut(s) 1881
DseDI GACNNNNNNGTC 1 cut(s) 1881
EaeI YGGCCR 3 cut(s) 3, 19, 1006
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 7 cut(s) 558, 881, 1059, 1668, 1857, 2021, 2955
EarI CTCTTC 7 cut(s) 558, 881, 1059, 1668, 1857, 2021, 2955
EciI GGCGGA 1 cut(s) 1121
Ecl136II GAGCTC 2 cut(s) 103, 568
EclXI CGGCCG 1 cut(s) 19
Eco105I TACGTA 1 cut(s) 1373
Eco130I CCWWGG 2 cut(s) 280, 1604
Eco24I GRGCYC 3 cut(s) 105, 570, 632
Eco31I GGTCTC 2 cut(s) 192, 1056
Eco32I GATATC 2 cut(s) 342, 3097
Eco47I GGWCC 1 cut(s) 1418
Eco52I CGGCCG 1 cut(s) 19
Eco53kI GAGCTC 2 cut(s) 103, 568
Eco57I CTGAAG 4 cut(s) 1040, 1559, 1811, 2985
Eco88I CYCGRG 1 cut(s) 189
EcoICRI GAGCTC 2 cut(s) 103, 568
EcoRI GAATTC 1 cut(s) 1779
EcoRII CCWGG 3 cut(s) 1698, 2552, 2862
EcoRV GATATC 2 cut(s) 342, 3097
EcoT14I CCWWGG 2 cut(s) 280, 1604
EcoT22I ATGCAT 1 cut(s) 2872
EcoT38I GRGCYC 3 cut(s) 105, 570, 632
ErhI CCWWGG 2 cut(s) 280, 1604
Esp3I CGTCTC 2 cut(s) 1889, 2139
FalI AAGNNNNNCTT 6 cut(s) 969, 1001, 1784, 1816, 1977, 2009
FauNDI CATATG 1 cut(s) 2623
FbaI TGATCA 2 cut(s) 616, 838
FblI GTMKAC 4 cut(s) 215, 753, 762, 814
Fnu4HI GCNGC 8 cut(s) 22, 25, 554, 722, 1009, 2878, 3134, 3144
FriOI GRGCYC 3 cut(s) 105, 570, 632
Fsp4HI GCNGC 8 cut(s) 22, 25, 554, 722, 1009, 2878, 3134, 3144
FspBI CTAG 8 cut(s) 87, 399, 962, 992, 1047, 1283, 2072, 2339
GlaI GCGC 3 cut(s) 2036, 2756, 2943
GluI GCNGC 8 cut(s) 22, 25, 554, 722, 1009, 2878, 3134, 3144
GsuI CTGGAG 4 cut(s) 86, 758, 2000, 2511
HaeII RGCGCY 1 cut(s) 2758
HaeIII GGCC 5 cut(s) 5, 21, 542, 1008, 2294
HapII CCGG 7 cut(s) 18, 42, 61, 129, 403, 418, 2677
HgaI GACGC 2 cut(s) 967, 2049
HhaI GCGC 3 cut(s) 2037, 2757, 2944
Hin1I GRCGYC 1 cut(s) 2060
Hin6I GCGC 3 cut(s) 2035, 2755, 2942
HinP1I GCGC 3 cut(s) 2035, 2755, 2942
HindIII AAGCTT 5 cut(s) 734, 983, 1307, 2360, 2466
HpaII CCGG 7 cut(s) 18, 42, 61, 129, 403, 418, 2677
HphI GGTGA 5 cut(s) 118, 1253, 2519, 3076, 3118
Hpy166II GTNNAC 7 cut(s) 216, 754, 763, 815, 1100, 1217, 1940
Hpy8I GTNNAC 7 cut(s) 216, 754, 763, 815, 1100, 1217, 1940
HpyCH4III ACNGT 8 cut(s) 32, 367, 1162, 1687, 2087, 2128, 2459, 2884
HpyCH4IV ACGT 6 cut(s) 693, 1372, 1902, 2148, 2638, 2765
HpyF10VI GCNNNNNNNGC 7 cut(s) 87, 100, 430, 775, 1556, 1937, 2886
HpySE526I ACGT 6 cut(s) 693, 1372, 1902, 2148, 2638, 2765
Hsp92I GRCGYC 1 cut(s) 2060
HspAI GCGC 3 cut(s) 2035, 2755, 2942
KroI GCCGGC 1 cut(s) 17
KroNI GCCGGC 1 cut(s) 19
Ksp22I TGATCA 2 cut(s) 616, 838
LguI GCTCTTC 2 cut(s) 558, 2955
LmnI GCTCC 5 cut(s) 421, 573, 635, 2924, 3151
Lsp1109I GCAGC 4 cut(s) 565, 733, 2889, 3145
MaeI CTAG 8 cut(s) 87, 399, 962, 992, 1047, 1283, 2072, 2339
MaeII ACGT 6 cut(s) 693, 1372, 1902, 2148, 2638, 2765
MaeIII GTNAC 5 cut(s) 248, 1333, 1510, 2248, 2507
MbiI CCGCTC 1 cut(s) 334
MfeI CAATTG 5 cut(s) 1236, 1377, 1413, 1470, 2844
MflI RGATCY 5 cut(s) 412, 890, 1999, 2391, 3065
MhlI GDGCHC 6 cut(s) 105, 570, 632, 1432, 1647, 1942
MlyI GAGTC 4 cut(s) 196, 1520, 2214, 2279
MmeI TCCRAC 2 cut(s) 573, 2999
Mph1103I ATGCAT 1 cut(s) 2872
MroNI GCCGGC 1 cut(s) 17
MroXI GAANNNNTTC 3 cut(s) 2467, 2798, 2839
MspA1I CMGCKG 1 cut(s) 1957
MspCI CTTAAG 1 cut(s) 986
MspI CCGG 7 cut(s) 18, 42, 61, 129, 403, 418, 2677
MspR9I CCNGG 5 cut(s) 419, 1700, 2554, 2678, 2864
MunI CAATTG 5 cut(s) 1236, 1377, 1413, 1470, 2844
Mva1269I GAATGC 3 cut(s) 499, 2047, 2695
MvaI CCWGG 3 cut(s) 1700, 2554, 2864
MvnI CGCG 2 cut(s) 1831, 2889
MwoI GCNNNNNNNGC 7 cut(s) 87, 100, 430, 775, 1556, 1937, 2886
NaeI GCCGGC 1 cut(s) 19
NciI CCSGG 2 cut(s) 419, 2678
NdeI CATATG 1 cut(s) 2623
NgoMIV GCCGGC 1 cut(s) 17
NheI GCTAGC 2 cut(s) 398, 961
NlaIV GGNNCC 3 cut(s) 631, 3067, 3147
NmeAIII GCCGAG 3 cut(s) 31, 84, 1102
NmuCI GTSAC 2 cut(s) 1333, 2507
NruI TCGCGA 1 cut(s) 2889
NsiI ATGCAT 1 cut(s) 2872
NspI RCATGY 3 cut(s) 1091, 1548, 1630
PaeI GCATGC 1 cut(s) 1091
PaeR7I CTCGAG 1 cut(s) 189
PagI TCATGA 3 cut(s) 658, 2953, 3091
PciI ACATGT 1 cut(s) 1544
PciSI GCTCTTC 2 cut(s) 558, 2955
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 3 cut(s) 499, 2047, 2695
PdiI GCCGGC 1 cut(s) 19
PdmI GAANNNNTTC 3 cut(s) 2467, 2798, 2839
PfoI TCCNGGA 2 cut(s) 417, 2676
PinAI ACCGGT 1 cut(s) 128
PkrI GCNGC 8 cut(s) 23, 26, 555, 723, 1010, 2879, 3135, 3145
PleI GAGTC 4 cut(s) 196, 1520, 2213, 2278
PpsI GAGTC 4 cut(s) 196, 1520, 2213, 2278
Ppu21I YACGTR 1 cut(s) 1373
PscI ACATGT 1 cut(s) 1544
PshBI ATTAAT 2 cut(s) 2214, 2406
PsiI TTATAA 1 cut(s) 233
Psp124BI GAGCTC 2 cut(s) 105, 570
Psp6I CCWGG 3 cut(s) 1698, 2552, 2862
PspGI CCWGG 3 cut(s) 1698, 2552, 2862
PspN4I GGNNCC 3 cut(s) 631, 3067, 3147
PspPI GGNCC 1 cut(s) 1418
PstI CTGCAG 2 cut(s) 555, 2350
PsuI RGATCY 5 cut(s) 412, 890, 1999, 2391, 3065
RruI TCGCGA 1 cut(s) 2889
RsaI GTAC 6 cut(s) 1099, 1218, 1234, 1375, 2542, 3078
RsaNI GTAC 6 cut(s) 1098, 1217, 1233, 1374, 2541, 3077
SacI GAGCTC 2 cut(s) 105, 570
SapI GCTCTTC 2 cut(s) 558, 2955
SatI GCNGC 8 cut(s) 22, 25, 554, 722, 1009, 2878, 3134, 3144
Sau96I GGNCC 1 cut(s) 1418
ScaI AGTACT 1 cut(s) 2542
SchI GAGTC 4 cut(s) 196, 1520, 2214, 2279
ScrFI CCNGG 5 cut(s) 419, 1700, 2554, 2678, 2864
SduI GDGCHC 6 cut(s) 105, 570, 632, 1432, 1647, 1942
SfcI CTRYAG 5 cut(s) 551, 1482, 2127, 2346, 2458
Sfr274I CTCGAG 1 cut(s) 189
SinI GGWCC 1 cut(s) 1418
SlaI CTCGAG 1 cut(s) 189
SmlI CTYRAG 4 cut(s) 189, 986, 1310, 2435
SmoI CTYRAG 4 cut(s) 189, 986, 1310, 2435
SnaBI TACGTA 1 cut(s) 1373
SphI GCATGC 1 cut(s) 1091
SsiI CCGC 9 cut(s) 22, 25, 334, 1009, 1106, 1957, 2099, 2354, 3143
SspI AATATT 1 cut(s) 1615
SspMI CTAG 8 cut(s) 87, 399, 962, 992, 1047, 1283, 2072, 2339
SstI GAGCTC 2 cut(s) 105, 570
StyD4I CCNGG 5 cut(s) 417, 1698, 2552, 2676, 2862
StyI CCWWGG 2 cut(s) 280, 1604
TaaI ACNGT 8 cut(s) 32, 367, 1162, 1687, 2087, 2128, 2459, 2884
TaiI ACGT 6 cut(s) 696, 1375, 1905, 2151, 2641, 2768
TaqI TCGA 6 cut(s) 99, 190, 267, 582, 2203, 3045
TaqII GACCGA 1 cut(s) 1887
TatI WGTACW 3 cut(s) 1097, 1232, 2540
TauI GCSGC 4 cut(s) 24, 27, 1011, 3146
TscAI CASTG 6 cut(s) 1048, 2092, 2242, 2599, 2809, 2887
TseFI GTSAC 2 cut(s) 1333, 2507
TseI GCWGC 4 cut(s) 553, 721, 2877, 3133
Tsp45I GTSAC 2 cut(s) 1333, 2507
TspGWI ACGGA 1 cut(s) 1292
TspRI CASTG 6 cut(s) 1048, 2092, 2242, 2599, 2809, 2887
Vha464I CTTAAG 1 cut(s) 986
VneI GTGCAC 1 cut(s) 1938
VpaK11BI GGWCC 1 cut(s) 1418
VspI ATTAAT 2 cut(s) 2214, 2406
XapI RAATTY 3 cut(s) 70, 521, 1779
XbaI TCTAGA 1 cut(s) 1282
XceI RCATGY 3 cut(s) 1091, 1548, 1630
XhoI CTCGAG 1 cut(s) 189
XmiI GTMKAC 4 cut(s) 215, 753, 762, 814
XmnI GAANNNNTTC 3 cut(s) 2467, 2798, 2839
XspI CTAG 8 cut(s) 87, 399, 962, 992, 1047, 1283, 2072, 2339
ZrmI AGTACT 1 cut(s) 2542
Zsp2I ATGCAT 1 cut(s) 2872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.