Rh1DG386500

Structural maintenance of chromosomes protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003625
Physical Location & Seq
Forward (+)
60616282 .. 60630246
13965 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG386500.1

Sequence Viewer

Length: 2937 bp
ATGCAAGTGATTGAGAGAGCCAAGCACAATTACGCTGTTGTCCATGTTGAGTTGAAGAATCAAGGGGAGGATGCTTTCAAGCCTGATATTTATGGGGATGTGATAATCATAGAGCGCAAGATTTCTGGAGCTTCAAACGCCACTGTTTTGAAGGATCATCAAGGAAGAAAGGTTGCTAGTCGGAAGGAGGACCTCCGGGAGCTAATTGAACATTTTAGTATTGATGTTGAGAATCCATGTGTAATAATGGGCCAAGACAGAAGCAGAGAGTTTTTGCATTCTGGGAATGACAAAGATAAATTTAAGTTCTTTTACAAGGCCACACTTCTGCAGCAAGTTGAAGAGCTTTTGCAAGACATTGAGAAACAGTTGGAGAAGGCAAATGTTGTTGTTGATCAATTAGAGGGCTCCATAAGACCCATAGAAAGGGAACTTAATGAGTTACATGAAAAGATCAAAAACATGGAGCACATTGAAGAAATATCTCAACAAGCAAAGCAGCTAAAAAAGAAGCTTGCTTGGTCATGGGTATATGATGTAGACAGGCAACTCCAGCAAAAGAATGCAAAAATTGGAAAGCTGAAAAATCGTGTACCTCTCTGTCAAGCTAAAATTGATCAGTTTAGAGATGAAGTGGAGAAACTTAGCCAATGCTATACCTCGAAGAAATCTGAGATTGCAAGCATGGTGGAAAAGACCGCTGAAGTTAGGAGAATGAAGGACGAATTGCGACAGTCGCTAGCATCAGCTACAAAAGACAAGCTTAAGCTAGAAGAAGAATATGGCCGCAAAACTAACTGCATCCAGAAATTGACAAACTCTGTTAGGTCTCTTCAGCAACATATTCAGGATGCTGAGGAGCAGCATGCCAGAAATACACAGGCAGAAGAATCTGCAATGGAGGAAAAGCTGAAAGAACTGCAAAATGAGGTTGCTACTATTGAATCAATGCTTGCAAGGTTGCAGGAAGAAGAGAGTGCCTTGTCAATAAGTGCACATGAGACAATTACTGAAATTGGAAAATTAACTGAAATGATTCAAAGCAAAGAGAAGGAATGTCAAAAATACTCCAATAAAATCCGCGAGGTTCTGAGAAATCAAAGCAACAAGGTCACAGCTTTTGGTGGAGATAGAGTGATGGACCTTCTACGTATAATTGAGAGGAATCATCAGAGATTCAAAAGCCCTCCAATTGGTCCAATTGGTGCCCATTTGACTTTGAATAATGGAGATGAATGGGCTAGAACTGTTGAAAATGCTGTAGGAAGGCTGCTCAATGCTTTCATTGTAACAAATCATAAAGACTCTCTACTTCTCAGAACATGTGCAAGGGAAGCAAACTATAATCACCTTCAGATTATCATATATGACTTTTCATTACCAAGGTTGAATATTCCACCTCACATGCTTCCACAAACAAGGCACCCAACTACTCTTTCTCTTCTCCATTCTGAAAATCACACTGTGGTGAATGTCCTGGTAGATATGGGAAGTATTGAAAGGCAAGTTCTTGTTAAAACATATGAGGAGGGGAAGGAAGTTGCTTTTGACCAGAGGATCCCCAATCTGAAGGAGGTCTACACTTATGATGGGCGAAAGATGTTTTCGCGTGGTTCTGTCCAGACAGTTCTTCCTCCCATTAGGCATGATAGACCTGTTCGTCTCTGTGCTAATTATGATGTTCAAATCAAAGAGCTTAAAACATCAGCATCAGCTGTACAAAAAGAAGCTGAGGAGTGCAGGACGAGAAAAAGGGAAGAAGAAGACAAACTTTGGGATCTCAAAGAAAAACACCAAAGTGTGAAGAGGAGGCGTATGAATGCTGACCGTGATTTGGCGTCTAAGAGACTAGCTATTCAAGACAGTGCATATGATGCGGAAGCCAGTACATCACCTGCATCAACTGTTGATGAGCTCCATCGAGACATTTTGAAAGTCCAAGGGGAGATTCAAGAGAGGGAAATGTCGCTGAAAACCTTTCAAGTCAGAATTAGTGAAGCTGTGGCAAAGACAAATGATCTTAAAGTAACATTTGAGAATCTATGTGACTCAGCAAAAGGAGACATTGATGCCTTTGAGAAAGCAGAGAGAGATTTGATGGAGATAGAAACAAAACTAGGTTCTGCGCAAACGGAGAAGCTTCATTATGAAGGTCTAATGAAGACCAAGGTCCTTCATGAAATCAATGAAGCTGAAAAACAATATAAAGAGCTTGAGCATCACCGCGAGGACAACTGTAGAAAGGCTTCAATCCTCTGTCCTGAAAGTGAAATTACAGCTTTGGGTGATTGGGATGGTAGCACTCCAGAACAACTCAGTGCCCAATTAACCAGGCTGAATCAGAGACTTCAGCGAGAGAGTGAGAGATGCACTGAATCCATTGATGAGCTCCGAATGTCATATGAGAGAAAAGAACGTAAGATTTTAAGAAAACAGAAAACATACAGAGCTTTTCGAGAAAAGTTGAATGCTTGCCAGAAAGCCCTAAATTTGCGAAGTACAAAGTTCCAGAGAAATAAAGATTTACTTAAGCGCCAGATGACGTGGCGATTTAATTCTCATCTGGGAAGGAAAGGGTTCAGTGGGAAGATTAAGGTCAGTTATGAGGAGAGAGCCCTTTCAATTGAGGTAAAGATGCCCCAGGATTCATCAAGCAGCACTGTTCGCGACACTAGAGGGCTTTCAGGTGGGGAACGCTCCTTTTCAACATTGTGCTTTGCGCTGGCTCTTCATGATATGACTGAAGCCCCATTTAGAGCTATGGATGAGTTTGATGTATTTATGGATGCTGTAAGTCGGAAAATCAGCCTTGATACTCTTGTCGAGTTTGCATTGGCGCAAGGATCCCAATGGGTACTTATCACCCCTCATGATATCAGCATGGTGAAAAATGGGGATCGGATAAAGAAGCAGCAAATGGCGGCTCCTCGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_23 SMC_N

Protein Analysis

978

Amino Acids

112.98

Weight (kDa)

8.28

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_N PF02463 6 - 971 9.4e-11 RecF/RecN/SMC N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1903
AasI GACNNNNNNGTC 1 cut(s) 1659
Acc16I TGCGCA 1 cut(s) 2127
Acc36I ACCTGC 1 cut(s) 1903
AccB1I GGYRCC 2 cut(s) 1205, 1422
AccI GTMKAC 2 cut(s) 540, 1578
AccII CGCG 4 cut(s) 1083, 1609, 2226, 2667
AciI CCGC 6 cut(s) 699, 787, 1081, 1877, 2224, 2921
AclWI GGATC 7 cut(s) 162, 1552, 1565, 1785, 2838, 2851, 2904
AcoI YGGCCR 1 cut(s) 784
AcsI RAATTY 2 cut(s) 299, 2488
AcuI CTGAAG 6 cut(s) 723, 818, 1337, 1589, 2333, 2763
AcyI GRCGYC 1 cut(s) 1838
AdeI CACNNNGTG 1 cut(s) 1465
AfaI GTAC 5 cut(s) 594, 1719, 1888, 2500, 2856
AfiI CCNNNNNNNGG 3 cut(s) 426, 1193, 1834
AflII CTTAAG 2 cut(s) 764, 2528
AflIII ACRYGT 1 cut(s) 1322
AjiI CACGTC 1 cut(s) 2544
AjnI CCWGG 3 cut(s) 1476, 2330, 2640
AjuI GAANNNNNNNTTGG 4 cut(s) 1420, 1452, 1932, 1964
AleI CACNNNNGTG 2 cut(s) 1466, 1797
Alw21I GWGCWC 4 cut(s) 471, 997, 1917, 2391
Alw26I GTCTC 7 cut(s) 834, 995, 1667, 1840, 1917, 2055, 2338
Alw44I GTGCAC 1 cut(s) 993
AlwI GGATC 7 cut(s) 162, 1552, 1565, 1785, 2838, 2851, 2904
AoxI GGCC 3 cut(s) 250, 318, 784
ApaLI GTGCAC 1 cut(s) 993
ApeKI GCWGC 6 cut(s) 331, 499, 862, 1270, 2655, 2911
ApoI RAATTY 2 cut(s) 299, 2488
Asp700I GAANNNNTTC 3 cut(s) 1035, 2245, 2576
AspLEI GCGC 5 cut(s) 117, 2128, 2535, 2722, 2839
AspS9I GGNCC 5 cut(s) 190, 250, 1141, 1196, 2170
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 7 cut(s) 1340, 1480, 1884, 2213, 2297, 2854, 2896
AsuNHI GCTAGC 1 cut(s) 739
AvaII GGWCC 4 cut(s) 190, 1141, 1196, 2170
BaeGI GKGCMC 3 cut(s) 997, 1210, 2323
BaeI ACNNNNGTAYC 2 cut(s) 2805, 2838
BamHI GGATCC 2 cut(s) 1557, 2843
BanI GGYRCC 2 cut(s) 1205, 1422
BanII GRGCYC 4 cut(s) 410, 1917, 2391, 2617
BarI GAAGNNNNNNTAC 4 cut(s) 1562, 1594, 2230, 2262
BbsI GAAGAC 2 cut(s) 1770, 2168
Bbv12I GWGCWC 4 cut(s) 471, 997, 1917, 2391
BbvCI CCTCAGC 2 cut(s) 855, 1731
BbvI GCAGC 6 cut(s) 343, 511, 874, 1257, 2667, 2923
BccI CCATC 5 cut(s) 1132, 1583, 1926, 2092, 2288
BciT130I CCWGG 3 cut(s) 1478, 2332, 2642
BclI TGATCA 2 cut(s) 394, 616
BcnI CCSGG 1 cut(s) 197
BcoDI GTCTC 7 cut(s) 834, 995, 1667, 1840, 1917, 2055, 2338
BfaI CTAG 7 cut(s) 177, 740, 770, 1242, 1850, 2117, 2673
BfmI CTRYAG 3 cut(s) 329, 1260, 2236
BfoI RGCGCY 1 cut(s) 2536
BfrI CTTAAG 2 cut(s) 764, 2528
BfuAI ACCTGC 1 cut(s) 1903
BisI GCNGC 8 cut(s) 332, 500, 787, 863, 1271, 2656, 2912, 2922
BlsI GCNGC 8 cut(s) 333, 501, 788, 864, 1272, 2657, 2913, 2923
Bme1390I CCNGG 4 cut(s) 197, 1478, 2332, 2642
Bme18I GGWCC 4 cut(s) 190, 1141, 1196, 2170
BmgBI CACGTC 1 cut(s) 2544
BmgT120I GGNCC 5 cut(s) 190, 250, 1141, 1196, 2170
BmiI GGNNCC 6 cut(s) 409, 1207, 1424, 1559, 2845, 2925
BmrFI CCNGG 4 cut(s) 197, 1478, 2332, 2642
BmtI GCTAGC 1 cut(s) 743
BoxI GACNNNNGTC 1 cut(s) 2168
BpiI GAAGAC 2 cut(s) 1770, 2168
BpmI CTGGAG 3 cut(s) 147, 536, 2289
Bpu10I CCTNAGC 2 cut(s) 855, 1731
BpuEI CTTGAG 1 cut(s) 2234
BpuMI CCSGG 1 cut(s) 197
BsaAI YACGTR 1 cut(s) 1151
BsaHI GRCGYC 1 cut(s) 1838
BsaI GGTCTC 1 cut(s) 834
BsaJI CCNNGG 4 cut(s) 1382, 1939, 2166, 2640
BsaXI ACNNNNNCTCC 2 cut(s) 1119, 1149
Bsc4I CCNNNNNNNGG 3 cut(s) 426, 1193, 1834
Bse1I ACTGG 1 cut(s) 1884
Bse3DI GCAATG 1 cut(s) 903
BseBI CCWGG 3 cut(s) 1478, 2332, 2642
BseDI CCNNGG 4 cut(s) 1382, 1939, 2166, 2640
BseGI GGATG 7 cut(s) 76, 103, 801, 856, 2299, 2770, 2791
BseLI CCNNNNNNNGG 3 cut(s) 426, 1193, 1834
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 7 cut(s) 663, 846, 1082, 1330, 1722, 2064, 2329
BseNI ACTGG 1 cut(s) 1884
BseRI GAGGAG 6 cut(s) 872, 1541, 1748, 1822, 2621, 2916
BseSI GKGCMC 3 cut(s) 997, 1210, 2323
BseXI GCAGC 6 cut(s) 343, 511, 874, 1257, 2667, 2923
BsgI GTGCAG 1 cut(s) 1759
Bsh1236I CGCG 4 cut(s) 1083, 1609, 2226, 2667
BshFI GGCC 3 cut(s) 252, 320, 786
BshNI GGYRCC 2 cut(s) 1205, 1422
BsiHKAI GWGCWC 4 cut(s) 471, 997, 1917, 2391
BsiSI CCGG 1 cut(s) 196
BslI CCNNNNNNNGG 3 cut(s) 426, 1193, 1834
BsmAI GTCTC 7 cut(s) 834, 995, 1667, 1840, 1917, 2055, 2338
BsmBI CGTCTC 1 cut(s) 1667
BsmI GAATGC 4 cut(s) 277, 568, 1825, 2473
BsnI GGCC 3 cut(s) 252, 320, 786
Bso31I GGTCTC 1 cut(s) 834
Bsp1286I GDGCHC 8 cut(s) 410, 471, 997, 1210, 1917, 2323, 2391, 2617
Bsp1407I TGTACA 1 cut(s) 1717
Bsp143I GATC 9 cut(s) 154, 394, 453, 616, 1557, 1777, 2017, 2843, 2896
Bsp68I TCGCGA 1 cut(s) 2667
BspACI CCGC 6 cut(s) 699, 787, 1081, 1877, 2224, 2921
BspANI GGCC 3 cut(s) 252, 320, 786
BspCNI CTCAG 7 cut(s) 664, 847, 1083, 1329, 1723, 2063, 2328
BspFNI CGCG 4 cut(s) 1083, 1609, 2226, 2667
BspHI TCATGA 3 cut(s) 2176, 2731, 2869
BspLI GGNNCC 6 cut(s) 409, 1207, 1424, 1559, 2845, 2925
BspMAI CTGCAG 1 cut(s) 333
BspMI ACCTGC 1 cut(s) 1903
BspOI GCTAGC 1 cut(s) 743
BspPI GGATC 7 cut(s) 162, 1552, 1565, 1785, 2838, 2851, 2904
BspQI GCTCTTC 2 cut(s) 336, 2733
BspT107I GGYRCC 2 cut(s) 1205, 1422
BspTI CTTAAG 2 cut(s) 764, 2528
BspTNI GGTCTC 1 cut(s) 834
BsrDI GCAATG 1 cut(s) 903
BsrGI TGTACA 1 cut(s) 1717
BsrI ACTGG 1 cut(s) 1884
BssECI CCNNGG 4 cut(s) 1382, 1939, 2166, 2640
BssMI GATC 9 cut(s) 154, 394, 453, 616, 1557, 1777, 2017, 2843, 2896
BssNI GRCGYC 1 cut(s) 1838
BssT1I CCWWGG 3 cut(s) 1382, 1939, 2166
Bst2UI CCWGG 3 cut(s) 1478, 2332, 2642
Bst6I CTCTTC 6 cut(s) 336, 837, 966, 1446, 1799, 2733
BstACI GRCGYC 1 cut(s) 1838
BstAFI CTTAAG 2 cut(s) 764, 2528
BstAPI GCANNNNNTGC 1 cut(s) 1874
BstAUI TGTACA 1 cut(s) 1717
BstBAI YACGTR 1 cut(s) 1151
BstC8I GCNNGC 7 cut(s) 516, 682, 741, 867, 954, 2473, 2724
BstDEI CTNAG 9 cut(s) 644, 672, 855, 1091, 1316, 1731, 1842, 2050, 2315
BstF5I GGATG 7 cut(s) 76, 103, 801, 856, 2299, 2770, 2791
BstFNI CGCG 4 cut(s) 1083, 1609, 2226, 2667
BstH2I RGCGCY 1 cut(s) 2536
BstHHI GCGC 5 cut(s) 117, 2128, 2535, 2722, 2839
BstKTI GATC 9 cut(s) 157, 397, 456, 619, 1560, 1780, 2020, 2846, 2899
BstMAI GTCTC 7 cut(s) 834, 995, 1667, 1840, 1917, 2055, 2338
BstMBI GATC 9 cut(s) 154, 394, 453, 616, 1557, 1777, 2017, 2843, 2896
BstMWI GCNNNNNNNGC 6 cut(s) 137, 553, 736, 1334, 1874, 2664
BstNI CCWGG 3 cut(s) 1478, 2332, 2642
BstNSI RCATGY 3 cut(s) 869, 1326, 1408
BstPAI GACNNNNGTC 1 cut(s) 2168
BstSCI CCNGG 4 cut(s) 195, 1476, 2330, 2640
BstSFI CTRYAG 3 cut(s) 329, 1260, 2236
BstSLI GKGCMC 3 cut(s) 997, 1210, 2323
BstSNI TACGTA 1 cut(s) 1151
BstUI CGCG 4 cut(s) 1083, 1609, 2226, 2667
BstV1I GCAGC 6 cut(s) 343, 511, 874, 1257, 2667, 2923
BstV2I GAAGAC 2 cut(s) 1770, 2168
BstX2I RGATCY 3 cut(s) 1557, 1777, 2843
BstYI RGATCY 3 cut(s) 1557, 1777, 2843
BsuRI GGCC 3 cut(s) 252, 320, 786
BtrI CACGTC 1 cut(s) 2544
BtsCI GGATG 7 cut(s) 76, 103, 801, 856, 2299, 2770, 2791
BtsIMutI CAGTG 7 cut(s) 141, 1461, 1870, 2323, 2370, 2587, 2658
BtuMI TCGCGA 1 cut(s) 2667
BveI ACCTGC 1 cut(s) 1903
Cac8I GCNNGC 7 cut(s) 516, 682, 741, 867, 954, 2473, 2724
CciI TCATGA 3 cut(s) 2176, 2731, 2869
CfoI GCGC 5 cut(s) 117, 2128, 2535, 2722, 2839
Cfr13I GGNCC 5 cut(s) 190, 250, 1141, 1196, 2170
CseI GACGC 1 cut(s) 1827
Csp6I GTAC 5 cut(s) 593, 1718, 1887, 2499, 2855
CspCI CAANNNNNGTGG 2 cut(s) 669, 704
CviQI GTAC 5 cut(s) 593, 1718, 1887, 2499, 2855
DdeI CTNAG 9 cut(s) 644, 672, 855, 1091, 1316, 1731, 1842, 2050, 2315
DpnI GATC 9 cut(s) 156, 396, 455, 618, 1559, 1779, 2019, 2845, 2898
DpnII GATC 9 cut(s) 154, 394, 453, 616, 1557, 1777, 2017, 2843, 2896
DraIII CACNNNGTG 1 cut(s) 1465
DrdI GACNNNNNNGTC 1 cut(s) 1659
DseDI GACNNNNNNGTC 1 cut(s) 1659
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 6 cut(s) 336, 837, 966, 1446, 1799, 2733
EarI CTCTTC 6 cut(s) 336, 837, 966, 1446, 1799, 2733
Ecl136II GAGCTC 2 cut(s) 1915, 2389
Eco105I TACGTA 1 cut(s) 1151
Eco130I CCWWGG 3 cut(s) 1382, 1939, 2166
Eco24I GRGCYC 4 cut(s) 410, 1917, 2391, 2617
Eco31I GGTCTC 1 cut(s) 834
Eco32I GATATC 1 cut(s) 2875
Eco47I GGWCC 4 cut(s) 190, 1141, 1196, 2170
Eco53kI GAGCTC 2 cut(s) 1915, 2389
Eco57I CTGAAG 6 cut(s) 723, 818, 1337, 1589, 2333, 2763
EcoICRI GAGCTC 2 cut(s) 1915, 2389
EcoO109I RGGNCCY 2 cut(s) 190, 2170
EcoRII CCWGG 3 cut(s) 1476, 2330, 2640
EcoRV GATATC 1 cut(s) 2875
EcoT14I CCWWGG 3 cut(s) 1382, 1939, 2166
EcoT38I GRGCYC 4 cut(s) 410, 1917, 2391, 2617
ErhI CCWWGG 3 cut(s) 1382, 1939, 2166
Esp3I CGTCTC 1 cut(s) 1667
FalI AAGNNNNNCTT 6 cut(s) 747, 779, 1755, 1787, 2511, 2543
FauNDI CATATG 3 cut(s) 1522, 1870, 2401
FbaI TGATCA 2 cut(s) 394, 616
FblI GTMKAC 2 cut(s) 540, 1578
Fnu4HI GCNGC 8 cut(s) 332, 500, 787, 863, 1271, 2656, 2912, 2922
FokI GGATG 7 cut(s) 83, 110, 788, 863, 2306, 2777, 2798
FriOI GRGCYC 4 cut(s) 410, 1917, 2391, 2617
Fsp4HI GCNGC 8 cut(s) 332, 500, 787, 863, 1271, 2656, 2912, 2922
FspBI CTAG 7 cut(s) 177, 740, 770, 1242, 1850, 2117, 2673
FspI TGCGCA 1 cut(s) 2127
GlaI GCGC 5 cut(s) 116, 2127, 2534, 2721, 2838
GluI GCNGC 8 cut(s) 332, 500, 787, 863, 1271, 2656, 2912, 2922
GsuI CTGGAG 3 cut(s) 147, 536, 2289
HaeII RGCGCY 1 cut(s) 2536
HaeIII GGCC 3 cut(s) 252, 320, 786
HapII CCGG 1 cut(s) 196
HgaI GACGC 1 cut(s) 1827
HhaI GCGC 5 cut(s) 117, 2128, 2535, 2722, 2839
Hin1I GRCGYC 1 cut(s) 1838
Hin6I GCGC 5 cut(s) 115, 2126, 2533, 2720, 2837
HinP1I GCGC 5 cut(s) 115, 2126, 2533, 2720, 2837
HindIII AAGCTT 3 cut(s) 512, 761, 2138
HpaII CCGG 1 cut(s) 196
HphI GGTGA 7 cut(s) 1340, 1480, 1884, 2213, 2297, 2854, 2896
Hpy166II GTNNAC 4 cut(s) 541, 593, 995, 1579
Hpy8I GTNNAC 4 cut(s) 541, 593, 995, 1579
HpyCH4IV ACGT 3 cut(s) 1150, 2416, 2543
HpyF10VI GCNNNNNNNGC 6 cut(s) 137, 553, 736, 1334, 1874, 2664
HpyF3I CTNAG 9 cut(s) 644, 672, 855, 1091, 1316, 1731, 1842, 2050, 2315
HpySE526I ACGT 3 cut(s) 1150, 2416, 2543
Hsp92I GRCGYC 1 cut(s) 1838
HspAI GCGC 5 cut(s) 115, 2126, 2533, 2720, 2837
Ksp22I TGATCA 2 cut(s) 394, 616
Kzo9I GATC 9 cut(s) 154, 394, 453, 616, 1557, 1777, 2017, 2843, 2896
LguI GCTCTTC 2 cut(s) 336, 2733
LmnI GCTCC 9 cut(s) 128, 199, 413, 466, 859, 1920, 2394, 2702, 2929
Lsp1109I GCAGC 6 cut(s) 343, 511, 874, 1257, 2667, 2923
MaeI CTAG 7 cut(s) 177, 740, 770, 1242, 1850, 2117, 2673
MaeII ACGT 3 cut(s) 1150, 2416, 2543
MaeIII GTNAC 5 cut(s) 441, 1111, 1288, 2026, 2045
MalI GATC 9 cut(s) 156, 396, 455, 618, 1559, 1779, 2019, 2845, 2898
MboI GATC 9 cut(s) 154, 394, 453, 616, 1557, 1777, 2017, 2843, 2896
MfeI CAATTG 3 cut(s) 1191, 1200, 2622
MflI RGATCY 3 cut(s) 1557, 1777, 2843
MhlI GDGCHC 8 cut(s) 410, 471, 997, 1210, 1917, 2323, 2391, 2617
MlyI GAGTC 2 cut(s) 1298, 2042
MmeI TCCRAC 3 cut(s) 161, 351, 2777
MroXI GAANNNNTTC 3 cut(s) 1035, 2245, 2576
MslI CAYNNNNRTG 2 cut(s) 1466, 1797
MspA1I CMGCKG 2 cut(s) 701, 1715
MspCI CTTAAG 2 cut(s) 764, 2528
MspI CCGG 1 cut(s) 196
MspR9I CCNGG 4 cut(s) 197, 1478, 2332, 2642
MunI CAATTG 3 cut(s) 1191, 1200, 2622
Mva1269I GAATGC 4 cut(s) 277, 568, 1825, 2473
MvaI CCWGG 3 cut(s) 1478, 2332, 2642
MvnI CGCG 4 cut(s) 1083, 1609, 2226, 2667
MwoI GCNNNNNNNGC 6 cut(s) 137, 553, 736, 1334, 1874, 2664
NciI CCSGG 1 cut(s) 197
NdeI CATATG 3 cut(s) 1522, 1870, 2401
NdeII GATC 9 cut(s) 154, 394, 453, 616, 1557, 1777, 2017, 2843, 2896
NheI GCTAGC 1 cut(s) 739
NlaIV GGNNCC 6 cut(s) 409, 1207, 1424, 1559, 2845, 2925
NmuCI GTSAC 2 cut(s) 1111, 2045
NruI TCGCGA 1 cut(s) 2667
NsbI TGCGCA 1 cut(s) 2127
NspI RCATGY 3 cut(s) 869, 1326, 1408
OliI CACNNNNGTG 2 cut(s) 1466, 1797
PaeI GCATGC 1 cut(s) 869
PagI TCATGA 3 cut(s) 2176, 2731, 2869
PaqCI CACCTGC 1 cut(s) 1903
PciI ACATGT 1 cut(s) 1322
PciSI GCTCTTC 2 cut(s) 336, 2733
PctI GAATGC 4 cut(s) 277, 568, 1825, 2473
PdmI GAANNNNTTC 3 cut(s) 1035, 2245, 2576
PfoI TCCNGGA 1 cut(s) 195
PkrI GCNGC 8 cut(s) 333, 501, 788, 864, 1272, 2657, 2913, 2923
PleI GAGTC 2 cut(s) 1298, 2042
PpsI GAGTC 2 cut(s) 1298, 2042
Ppu21I YACGTR 1 cut(s) 1151
PpuMI RGGWCCY 2 cut(s) 190, 2170
PscI ACATGT 1 cut(s) 1322
PshAI GACNNNNGTC 1 cut(s) 2168
Psp124BI GAGCTC 2 cut(s) 1917, 2391
Psp5II RGGWCCY 2 cut(s) 190, 2170
Psp6I CCWGG 3 cut(s) 1476, 2330, 2640
PspGI CCWGG 3 cut(s) 1476, 2330, 2640
PspN4I GGNNCC 6 cut(s) 409, 1207, 1424, 1559, 2845, 2925
PspPI GGNCC 5 cut(s) 190, 250, 1141, 1196, 2170
PspPPI RGGWCCY 2 cut(s) 190, 2170
PstI CTGCAG 1 cut(s) 333
PsuI RGATCY 3 cut(s) 1557, 1777, 2843
PvuII CAGCTG 1 cut(s) 1715
RruI TCGCGA 1 cut(s) 2667
RsaI GTAC 5 cut(s) 594, 1719, 1888, 2500, 2856
RsaNI GTAC 5 cut(s) 593, 1718, 1887, 2499, 2855
RseI CAYNNNNRTG 2 cut(s) 1466, 1797
SacI GAGCTC 2 cut(s) 1917, 2391
SapI GCTCTTC 2 cut(s) 336, 2733
SatI GCNGC 8 cut(s) 332, 500, 787, 863, 1271, 2656, 2912, 2922
Sau3AI GATC 9 cut(s) 154, 394, 453, 616, 1557, 1777, 2017, 2843, 2896
Sau96I GGNCC 5 cut(s) 190, 250, 1141, 1196, 2170
SchI GAGTC 2 cut(s) 1298, 2042
ScrFI CCNGG 4 cut(s) 197, 1478, 2332, 2642
SduI GDGCHC 8 cut(s) 410, 471, 997, 1210, 1917, 2323, 2391, 2617
SfcI CTRYAG 3 cut(s) 329, 1260, 2236
SinI GGWCC 4 cut(s) 190, 1141, 1196, 2170
SmiMI CAYNNNNRTG 2 cut(s) 1466, 1797
SmlI CTYRAG 3 cut(s) 764, 2213, 2528
SmoI CTYRAG 3 cut(s) 764, 2213, 2528
SnaBI TACGTA 1 cut(s) 1151
SphI GCATGC 1 cut(s) 869
SsiI CCGC 6 cut(s) 699, 787, 1081, 1877, 2224, 2921
SspI AATATT 1 cut(s) 1393
SspMI CTAG 7 cut(s) 177, 740, 770, 1242, 1850, 2117, 2673
SstI GAGCTC 2 cut(s) 1917, 2391
StyD4I CCNGG 4 cut(s) 195, 1476, 2330, 2640
StyI CCWWGG 3 cut(s) 1382, 1939, 2166
TaiI ACGT 3 cut(s) 1153, 2419, 2546
TaqI TCGA 4 cut(s) 662, 1921, 2455, 2823
TatI WGTACW 3 cut(s) 1717, 1886, 2498
TauI GCSGC 2 cut(s) 789, 2924
TscAI CASTG 7 cut(s) 148, 1468, 1870, 2323, 2377, 2587, 2665
TseFI GTSAC 2 cut(s) 1111, 2045
TseI GCWGC 6 cut(s) 331, 499, 862, 1270, 2655, 2911
Tsp45I GTSAC 2 cut(s) 1111, 2045
TspGWI ACGGA 1 cut(s) 2147
TspRI CASTG 7 cut(s) 148, 1468, 1870, 2323, 2377, 2587, 2665
Vha464I CTTAAG 2 cut(s) 764, 2528
VneI GTGCAC 1 cut(s) 993
VpaK11BI GGWCC 4 cut(s) 190, 1141, 1196, 2170
XapI RAATTY 2 cut(s) 299, 2488
XceI RCATGY 3 cut(s) 869, 1326, 1408
XmiI GTMKAC 2 cut(s) 540, 1578
XmnI GAANNNNTTC 3 cut(s) 1035, 2245, 2576
XspI CTAG 7 cut(s) 177, 740, 770, 1242, 1850, 2117, 2673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.