FvH4_7g27350

Structural maintenance of chromosomes protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003832
Physical Location & Seq
Reverse (-)
20464391 .. 20470204
5814 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27350.t1

Sequence Viewer

Length: 1491 bp
ATGCAGGCAACAATAAAGGAAATGGAAACACAAGTGTCCAAGTTGACTGCTGAAAAGGAGGCTAGTATGGGTGGAGAAGTGAAAAATCTGTCTAACCAAGTAGATGCGCTTTCTCAAGATGTAGTGAGGGAAGTGTCTATACTGGATAATATGAAGGACAATCTTGATACTGAAAATGATAATGCTAGAATGATCGCTAGCAACATTGAAGACATGAAACAATCTCTAAAAGATAGGGACTGTGCTATAAGAAATGCCGAAGAAGGAGCTGCTGATTTGAAAAAGAGAGCGGAGGAACTTTCTCATAGTCTGAATGAGTATGAGACTCAGTACCAGGGTGTAATCGCTGGCAAGAGTAGTGGAAATGAGGATAAATGCCTCGAAGATCAATTGGGTGATGCCAAAAGAGCTGTAGGGAGTGCTGAAACAGAACTGGAACAGTTGAAAACAAAAATTCGTCACTGTGAAAAGGAGTTGAAAGAGAAAAGTTCTCAACTAATGTCAAAGCGTGAAGAAGCTGTTGCTGTAGAGAGTGAGCTGAAGGCTAGGAAAACAGATGTTGAAAATGTTAAATTGGCACTGGAGTCTCTTCCATATAAGGAGGGCCAGATGGAAGCTTTGCAAAAGGACCGATCATCTGAAGATCCTGTGAACAATTTTGTTAGGTCTAAGGTCAAGGGTGTAGTTGCAAAACTTATCAAAGTGAAGGATAGCTCCACCATGACTGCCTTAGAGGTCACTGCTGGTGGAAAGTTGTTTAATGTTGTCGTAGACACAGAAAGTACTGGAAAACAACTTCTTCAGAATGGCAATCTTCGAAGAAGAGTAACAATCATACCCTTGAACAAAATTCAAGCTCACACAGTTCCTCCTAGGGTTCAAAATGCTGCTGCTAAATTGGTTGGCAGAGAAAACGCAGAACTTGCACTTTCTTTGGTTGGCTATGATGAGCACCTGAGGAGTGCTATGGAATATGTCTTCGGTTCAACCTTTGTGTGCAAGACTATTGATGCTGCAAAGGAGGTTGCTTTTAACAGGGAAGTTCGTACCCCTAGTGTCACTCTTGAAGGTGATATCTTTCAGCCCAGTGGTCTTTTGACTGGTGGAAGCCGCAAGGGTGGGGGTGATCTGTTAAGGCAGCGTCATGAGCTGGCTGAAGCTGAGCTAAAACTCTCTGAGCATCAGAAAAAGTTGACTGAGATTGAAGCTAAGATAAGAGAAATTCAGCCTCTTCACAAGAAGGCTGAACAAAATGAGCATCATAAGCTTGGTGAATTAGTAAAGAGGATTGAGCAAGAGCTACAAGAAGCTAATACTGCAGCCAAAGAAAAGAAACTTTTGCATGAAGATTGTGTGAATAAAGTGTCATTTCTTGAGAAATCAATCAAAGAGCACGACAACAGTCGGGAGGGAAGGCTGAAAGATTTGGAAAAGAAGATCAAAGAGACAAAAGCTCTACTCCAGTCAGCTTCAAAGGATCTCAAGCCTTGA

Protein Analysis

497

Amino Acids

55.32

Weight (kDa)

6.34

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_hinge PF06470 223 - 343 1.8e-24 SMC proteins Flexible Hinge Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 290
AccI GTMKAC 1 cut(s) 771
AciI CCGC 2 cut(s) 290, 1111
AclWI GGATC 2 cut(s) 638, 1485
AcsI RAATTY 3 cut(s) 453, 849, 1221
AcuI CTGAAG 4 cut(s) 560, 660, 785, 1176
AfaI GTAC 3 cut(s) 332, 784, 1048
AjnI CCWGG 1 cut(s) 333
Alw21I GWGCWC 2 cut(s) 954, 1395
Alw26I GTCTC 3 cut(s) 317, 591, 1439
AlwI GGATC 2 cut(s) 638, 1485
AoxI GGCC 1 cut(s) 604
ApeKI GCWGC 6 cut(s) 269, 887, 890, 1013, 1138, 1319
ApoI RAATTY 3 cut(s) 453, 849, 1221
AspA2I CCTAGG 1 cut(s) 872
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 2 cut(s) 604, 628
AsuHPI GGTGA 4 cut(s) 407, 1082, 1136, 1283
AsuII TTCGAA 1 cut(s) 817
AsuNHI GCTAGC 1 cut(s) 197
AvaII GGWCC 1 cut(s) 628
AvrII CCTAGG 1 cut(s) 872
AxyI CCTNAGG 1 cut(s) 956
BarI GAAGNNNNNNTAC 2 cut(s) 123, 155
BbsI GAAGAC 2 cut(s) 216, 970
Bbv12I GWGCWC 2 cut(s) 954, 1395
BbvI GCAGC 6 cut(s) 256, 874, 877, 1000, 1150, 1331
BccI CCATC 1 cut(s) 604
BciT130I CCWGG 1 cut(s) 335
BcoDI GTCTC 3 cut(s) 317, 591, 1439
BfaI CTAG 6 cut(s) 63, 186, 198, 546, 873, 1053
BfmI CTRYAG 3 cut(s) 411, 525, 1317
BisI GCNGC 7 cut(s) 270, 888, 891, 1014, 1111, 1139, 1320
BlnI CCTAGG 1 cut(s) 872
BlpI GCTNAGC 1 cut(s) 1161
BlsI GCNGC 7 cut(s) 271, 889, 892, 1015, 1112, 1140, 1321
BmcAI AGTACT 1 cut(s) 784
Bme1390I CCNGG 1 cut(s) 335
Bme18I GGWCC 1 cut(s) 628
BmgT120I GGNCC 2 cut(s) 604, 628
BmrFI CCNGG 1 cut(s) 335
BmrI ACTGGG 1 cut(s) 1080
BmsI GCATC 5 cut(s) 94, 388, 1000, 1189, 1267
BmtI GCTAGC 1 cut(s) 201
BmuI ACTGGG 1 cut(s) 1080
BoxI GACNNNNGTC 1 cut(s) 1401
BpiI GAAGAC 2 cut(s) 216, 970
BpmI CTGGAG 2 cut(s) 602, 1445
Bpu1102I GCTNAGC 1 cut(s) 1161
Bpu14I TTCGAA 1 cut(s) 817
BpuEI CTTGAG 3 cut(s) 99, 1394, 1466
BsaJI CCNNGG 2 cut(s) 334, 872
BsaXI ACNNNNNCTCC 2 cut(s) 853, 883
Bse1I ACTGG 7 cut(s) 147, 438, 585, 790, 1086, 1105, 1462
Bse21I CCTNAGG 1 cut(s) 956
BseBI CCWGG 1 cut(s) 335
BseDI CCNNGG 2 cut(s) 334, 872
BseMII CTCAG 5 cut(s) 341, 947, 1152, 1167, 1188
BseNI ACTGG 7 cut(s) 147, 438, 585, 790, 1086, 1105, 1462
BseRI GAGGAG 1 cut(s) 973
BseXI GCAGC 6 cut(s) 256, 874, 877, 1000, 1150, 1331
BshFI GGCC 1 cut(s) 606
BsiHKAI GWGCWC 2 cut(s) 954, 1395
BslFI GGGAC 1 cut(s) 251
BsmAI GTCTC 3 cut(s) 317, 591, 1439
BsmFI GGGAC 1 cut(s) 251
BsnI GGCC 1 cut(s) 606
Bsp119I TTCGAA 1 cut(s) 817
Bsp1286I GDGCHC 2 cut(s) 954, 1395
Bsp143I GATC 7 cut(s) 192, 385, 632, 643, 1126, 1437, 1477
Bsp1720I GCTNAGC 1 cut(s) 1161
BspACI CCGC 2 cut(s) 290, 1111
BspANI GGCC 1 cut(s) 606
BspCNI CTCAG 5 cut(s) 340, 948, 1153, 1168, 1189
BspHI TCATGA 1 cut(s) 1144
BspMAI CTGCAG 1 cut(s) 1321
BspOI GCTAGC 1 cut(s) 201
BspPI GGATC 2 cut(s) 638, 1485
BspT104I TTCGAA 1 cut(s) 817
BsrBI CCGCTC 1 cut(s) 290
BsrI ACTGG 7 cut(s) 147, 438, 585, 790, 1086, 1105, 1462
BssECI CCNNGG 2 cut(s) 334, 872
BssMI GATC 7 cut(s) 192, 385, 632, 643, 1126, 1437, 1477
BssT1I CCWWGG 1 cut(s) 872
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 5 cut(s) 242, 441, 464, 865, 1403
Bst6I CTCTTC 3 cut(s) 594, 817, 1236
BstAPI GCANNNNNTGC 1 cut(s) 923
BstBI TTCGAA 1 cut(s) 817
BstC8I GCNNGC 4 cut(s) 6, 199, 349, 1152
BstDEI CTNAG 8 cut(s) 327, 669, 730, 956, 1161, 1176, 1197, 1209
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 7 cut(s) 195, 388, 635, 646, 1129, 1440, 1480
BstMAI GTCTC 3 cut(s) 317, 591, 1439
BstMBI GATC 7 cut(s) 192, 385, 632, 643, 1126, 1437, 1477
BstMWI GCNNNNNNNGC 5 cut(s) 407, 923, 1147, 1264, 1316
BstNI CCWGG 1 cut(s) 335
BstPAI GACNNNNGTC 1 cut(s) 1401
BstSCI CCNGG 1 cut(s) 333
BstSFI CTRYAG 3 cut(s) 411, 525, 1317
BstV1I GCAGC 6 cut(s) 256, 874, 877, 1000, 1150, 1331
BstV2I GAAGAC 2 cut(s) 216, 970
BstX2I RGATCY 2 cut(s) 643, 1477
BstYI RGATCY 2 cut(s) 643, 1477
Bsu36I CCTNAGG 1 cut(s) 956
BsuRI GGCC 1 cut(s) 606
BtsI GCAGTG 1 cut(s) 738
BtsIMutI CAGTG 4 cut(s) 460, 578, 738, 1093
Cac8I GCNNGC 4 cut(s) 6, 199, 349, 1152
CciI TCATGA 1 cut(s) 1144
CfoI GCGC 1 cut(s) 109
Cfr13I GGNCC 2 cut(s) 604, 628
CseI GACGC 1 cut(s) 1130
Csp6I GTAC 3 cut(s) 331, 783, 1047
CspCI CAANNNNNGTGG 2 cut(s) 340, 375
CviAII CATG 4 cut(s) 214, 721, 1145, 1343
CviQI GTAC 3 cut(s) 331, 783, 1047
DdeI CTNAG 8 cut(s) 327, 669, 730, 956, 1161, 1176, 1197, 1209
DpnI GATC 7 cut(s) 194, 387, 634, 645, 1128, 1439, 1479
DpnII GATC 7 cut(s) 192, 385, 632, 643, 1126, 1437, 1477
Eam1104I CTCTTC 3 cut(s) 594, 817, 1236
EarI CTCTTC 3 cut(s) 594, 817, 1236
Eco130I CCWWGG 1 cut(s) 872
Eco32I GATATC 1 cut(s) 1075
Eco47I GGWCC 1 cut(s) 628
Eco57I CTGAAG 4 cut(s) 560, 660, 785, 1176
Eco81I CCTNAGG 1 cut(s) 956
EcoRII CCWGG 1 cut(s) 333
EcoRV GATATC 1 cut(s) 1075
EcoT14I CCWWGG 1 cut(s) 872
ErhI CCWWGG 1 cut(s) 872
FaeI CATG 4 cut(s) 217, 724, 1148, 1346
FaqI GGGAC 1 cut(s) 251
FatI CATG 4 cut(s) 213, 720, 1144, 1342
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 7 cut(s) 270, 888, 891, 1014, 1111, 1139, 1320
Fsp4HI GCNGC 7 cut(s) 270, 888, 891, 1014, 1111, 1139, 1320
FspBI CTAG 6 cut(s) 63, 186, 198, 546, 873, 1053
GlaI GCGC 1 cut(s) 108
GluI GCNGC 7 cut(s) 270, 888, 891, 1014, 1111, 1139, 1320
GsuI CTGGAG 2 cut(s) 602, 1445
HaeIII GGCC 1 cut(s) 606
HgaI GACGC 1 cut(s) 1130
HhaI GCGC 1 cut(s) 109
Hin1II CATG 4 cut(s) 217, 724, 1148, 1346
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 2 cut(s) 45, 1194
HindII GTYRAC 2 cut(s) 45, 1194
HindIII AAGCTT 2 cut(s) 615, 1265
HinfI GANTC 2 cut(s) 325, 584
HphI GGTGA 4 cut(s) 407, 1082, 1136, 1283
Hpy166II GTNNAC 4 cut(s) 45, 652, 772, 1194
Hpy188I TCNGA 5 cut(s) 312, 640, 804, 1177, 1185
Hpy188III TCNNGA 6 cut(s) 116, 164, 1064, 1145, 1373, 1406
Hpy8I GTNNAC 4 cut(s) 45, 652, 772, 1194
HpyAV CCTTC 7 cut(s) 148, 257, 535, 700, 1061, 1234, 1407
HpyCH4III ACNGT 5 cut(s) 242, 441, 464, 865, 1403
HpyCH4V TGCA 8 cut(s) 4, 622, 689, 926, 999, 1016, 1319, 1342
HpyF10VI GCNNNNNNNGC 5 cut(s) 407, 923, 1147, 1264, 1316
HpyF3I CTNAG 8 cut(s) 327, 669, 730, 956, 1161, 1176, 1197, 1209
Hsp92II CATG 4 cut(s) 217, 724, 1148, 1346
HspAI GCGC 1 cut(s) 107
Kzo9I GATC 7 cut(s) 192, 385, 632, 643, 1126, 1437, 1477
LmnI GCTCC 2 cut(s) 266, 719
Lsp1109I GCAGC 6 cut(s) 256, 874, 877, 1000, 1150, 1331
LweI GCATC 5 cut(s) 94, 388, 1000, 1189, 1267
MaeI CTAG 6 cut(s) 63, 186, 198, 546, 873, 1053
MaeIII GTNAC 4 cut(s) 458, 736, 826, 1057
MalI GATC 7 cut(s) 194, 387, 634, 645, 1128, 1439, 1479
MbiI CCGCTC 1 cut(s) 290
MboI GATC 7 cut(s) 192, 385, 632, 643, 1126, 1437, 1477
MfeI CAATTG 1 cut(s) 389
MflI RGATCY 2 cut(s) 643, 1477
MhlI GDGCHC 2 cut(s) 954, 1395
MluCI AATT 8 cut(s) 389, 453, 572, 655, 849, 896, 1221, 1274
MlyI GAGTC 2 cut(s) 319, 593
MseI TTAA 4 cut(s) 570, 759, 1032, 1133
MspR9I CCNGG 1 cut(s) 335
MunI CAATTG 1 cut(s) 389
MvaI CCWGG 1 cut(s) 335
MwoI GCNNNNNNNGC 5 cut(s) 407, 923, 1147, 1264, 1316
NdeII GATC 7 cut(s) 192, 385, 632, 643, 1126, 1437, 1477
NheI GCTAGC 1 cut(s) 197
NlaIII CATG 4 cut(s) 217, 724, 1148, 1346
NmuCI GTSAC 3 cut(s) 458, 736, 1057
NspV TTCGAA 1 cut(s) 817
PagI TCATGA 1 cut(s) 1144
PkrI GCNGC 7 cut(s) 271, 889, 892, 1015, 1112, 1140, 1321
PleI GAGTC 2 cut(s) 319, 592
PpsI GAGTC 2 cut(s) 319, 592
PshAI GACNNNNGTC 1 cut(s) 1401
Psp6I CCWGG 1 cut(s) 333
PspGI CCWGG 1 cut(s) 333
PspPI GGNCC 2 cut(s) 604, 628
PstI CTGCAG 1 cut(s) 1321
PsuI RGATCY 2 cut(s) 643, 1477
RsaI GTAC 3 cut(s) 332, 784, 1048
RsaNI GTAC 3 cut(s) 331, 783, 1047
SaqAI TTAA 4 cut(s) 570, 759, 1032, 1133
SatI GCNGC 7 cut(s) 270, 888, 891, 1014, 1111, 1139, 1320
Sau3AI GATC 7 cut(s) 192, 385, 632, 643, 1126, 1437, 1477
Sau96I GGNCC 2 cut(s) 604, 628
ScaI AGTACT 1 cut(s) 784
SchI GAGTC 2 cut(s) 319, 593
ScrFI CCNGG 1 cut(s) 335
SduI GDGCHC 2 cut(s) 954, 1395
SfaNI GCATC 5 cut(s) 94, 388, 1000, 1189, 1267
SfcI CTRYAG 3 cut(s) 411, 525, 1317
SfuI TTCGAA 1 cut(s) 817
SinI GGWCC 1 cut(s) 628
SmlI CTYRAG 3 cut(s) 114, 1373, 1481
SmoI CTYRAG 3 cut(s) 114, 1373, 1481
Sse9I AATT 8 cut(s) 389, 453, 572, 655, 849, 896, 1221, 1274
SsiI CCGC 2 cut(s) 290, 1111
SspMI CTAG 6 cut(s) 63, 186, 198, 546, 873, 1053
StyD4I CCNGG 1 cut(s) 333
StyI CCWWGG 1 cut(s) 872
TaaI ACNGT 5 cut(s) 242, 441, 464, 865, 1403
TaqI TCGA 2 cut(s) 381, 817
TaqII GACCGA 1 cut(s) 645
TasI AATT 8 cut(s) 389, 453, 572, 655, 849, 896, 1221, 1274
TatI WGTACW 1 cut(s) 782
TauI GCSGC 1 cut(s) 1113
Tru1I TTAA 4 cut(s) 570, 759, 1032, 1133
Tru9I TTAA 4 cut(s) 570, 759, 1032, 1133
TscAI CASTG 4 cut(s) 467, 585, 745, 1093
TseFI GTSAC 3 cut(s) 458, 736, 1057
TseI GCWGC 6 cut(s) 269, 887, 890, 1013, 1138, 1319
Tsp45I GTSAC 3 cut(s) 458, 736, 1057
TspDTI ATGAA 3 cut(s) 167, 230, 1359
TspRI CASTG 4 cut(s) 467, 585, 745, 1093
VpaK11BI GGWCC 1 cut(s) 628
XapI RAATTY 3 cut(s) 453, 849, 1221
XmaJI CCTAGG 1 cut(s) 872
XmiI GTMKAC 1 cut(s) 771
XspI CTAG 6 cut(s) 63, 186, 198, 546, 873, 1053
ZrmI AGTACT 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.