Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0003832

Physical Location & Seq

Genomic Coordinates Reverse (-)

32041998 .. 32048737

Gene Structure 6740 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1252800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3528 bp

ATGTACATCAAGGAGGTATGCCTGGAGGGCTTCAAGTCCTACGCGACGAGGACGGTGGTGCCCGGGTTCGACCCGTTTTTCAACGCCATAACGGGTCTGAACGGGTCGGGCAAGTCCAACATCCTCGACTCCATCTGCTTCGTCTTGGGAATTACCAATTTGCAGCAGGTTCGCGCCGCCAACCTCCAGGAGCTCGTCTACAAGCAGGGCCAGGCTGGAATCACCAAGGCCACCGTCTCCATAGTCTTCGACAATTCTGACCGCGCCCGTAGTCCCCTCGGATACGAGGCCCATTCTGAAATCACCGTCACCCGTCAGATTGTGGTTGGTGGAAGGAACAAGTATTTGATCAATGGGAAACTTGCACAGCCTAGTCAGGTCCAGAACCTTTTTCATTCCGTGCAGCTCAATGTTAACAACCCGCATTTTCTGATAATGCAAGGGCGCATTACCAAGGTTTTGAATATGAAACCACCCGAGATTCTGTCTATGCTTGAAGAGGCTGCTGGGACGAGAATGTATGAGACCAAGAAAGAGGCCGCTTTGAAGACACTTGAGAAGAAGCAGAGTAAAGTTGATGAGATCGATAAGCTTCTTGACCAGGAGATACTGCCTGCCTTGGACAAGTTAAGGAGAGAAAGGACGCAATATATGCAATGGGCTAATGGAAACGCTGACTTGGATCACCTGAAAAGGTTTTGCATTGCGCTTGAATATGTTCAAGCAGAAAGAATTAGAGACGGTGCAGCATCTGAGTTGGAACAAGTTAAGGCCAGGATTTCCGAGGTTGATGATGATATGAGAAAGATGAAGGGAGAAATACAGGAAATGGAGACACAAGTGTTGAAGTTGACTGCTGACAAGGAGGCTAGAATGGGTGGGGAAGTAAAAACTTTGTCTGACAAGGTAGATGCTCTTTCCCAAGATCTTGTGAGGGAAGTGTCTGTGCTGAGCAATAAAGAGGATAATCTTGGTATTGAAAATGAAAATGCGGAAAAGATTGTTAACAATATTGAAGACATGAAGCAGTCTGTAAAAGAGATGGACTTCGCTATAAGAAAGGCTGAAGAAGGAGCAGCTGATTTGAAAAAGAGAGCAGAGGAACTTTCTCAGACACTGAATGAGTATGAAAAGGATTACCAGGGTGTACTAGCTGGCAAGAGCAGTGGAAATGAGGAGAAATGCCTCGAAGATCAACTGGGTGATGCGAAAGTAGCTGTTGGGAGTGCTGAAACAGAACTAAAACAGCTGAACACAAAAATAAGCCATTGTCAAAGGGAGTTAAAAGAGAAAAATAAGCAGCTAATGTCAAAGCGTGAAGAAGCTGATGCGGTAAAGAGTGAACTTACTGTTAGACAACGAGATTTGGAAAATCTTGAAAATGCACTGAAGTCTATTCCATATAAAGAGGGCCAGATGGAAGCATTACAAAAGGATCGTGCTTCTGAGTTAGGGGAGGTGCAGAAGCTGAGGGATGAAATGCGAAATCTTTCAGCTCAATTATCAAATGTTGAGTTCAACTATCGGGATCCCGTGAATAACTTTGATAGGTCTAAGGTCAAGGGTGTAGTTGCAAAACTTATCAAAGTAAAGGATAGCTCGACGATGACTGCCTTAGAGGTTACTGCTGGTGGAAAGTTGTTTAATGTTGTTGTAGACACGGAAAGTACTGGAAAACAACTTCTTCAGAATGGCAACCTTCGGAGAAGAGTAACAATTATACCTCTGAACAAAATACAACCGCATACTGTTCATCCTAGGGTTCAACAAGCTGCTTCTAAATTGGTTGGCAAGGAGAATGCAGAGCTTGCACTTTCTTTGGTTGGGTATGATAATGAATTGAAGAATGCTATGGAATTTGTTTTTGGTTCAACCTTTGTTTGCAAAACCGTAGATGCTGCAAAGGAGGTTGCTTTTAACAGGGAAATTCGCAGCCCTAGTGTCACTCTTGAAGGTGATATCTTTCAGCCTAGTGGCCTCTTGACTGGTGGAAGCCGCAAGGGTGGGGGTGATTTGTTAAAGCAACTTCACGAGCTGGCAGAGTCTGAACAAAAACTTTCCATGCATCAGAGAAGATTGACCGAAATTGAAGCCAGGATTACAGAGCTTCTGCCTCTTCAGAAGAAGTTCATGGACCTTAAAGCACAGTTAGAACTTAAATCCTATGACCTTTCATTATTCCAGGGCAGGGCTGAGCAAAATGAGCATCATAAGCTTGGTGAATTAGTAAAAAGGATCGAGCAGGAACTTCAAGAAGCACAATCTGCAGCAAAAGAAAAGCAGCTTTTGTACGAAGAGTGTGTGAATAAAGTTACATTACTTGAGAAATCAATCAAAGAGAATGATAATAGTCGGGAGGGAAGGCTCAAAGATTTTGAAAAGAAGATTAAAGAAACAAAAGCTCAAATGCAATCAGCTTCAAGGAATCTAAAGGGGCATGAGAATGAAAAAGAGAAGCTTATTATGGAAAAAGAAGCTGCTATAAAGGAACTTGCATCTTCAGAAACTCAATTAGCTTCTTTGAGAACACAAATTGACAACCTAACTTCAGAAGTCGAAGAACAGAGAGCAAAGGTAGCTTCTACAAGAAATATTCATGATAGTGCCCAATCTGAGCTTAACTCAATTCGCATGAAGATGAAAGATTGTGATTCCCAAATTAGTGGCATTCTTAAGGAGCAACAAACACTTCAGCGTAAACTTAGTGAGACAAATCTTGAAAGGAAGAAAATGGAAAATGAGGTAAAACGAATGGAAACGGAGCAGAAAGATTGCTCTACCAAAGTTGATAAATTGATAGAGAAGCATGCCTGGATTGTATCTGAGAAACAGTTATTTGGTAAAAGTGGGACTGATTATGATTTTTCCTTGCGGGATCCTCATAATGCAAGGGAAGAACTTGAGAGACTGCAGGCCCAACAGTCTGGCCTCGAGAAAAGGGTGAATAAGAAAGTTATGGCAATGTTTGAGAAAGCAGAAGATGAGTACAACGATCTAATGTCTAAGAAGGACATCATTGAGAATGATAAGTCTAAGATCAAGAAGGTGATTGAAGAGCTGGATGAGAAAAAGAAGGAAACACTGAAAGTTACTTGGGTAAAAGTTAATAATGACTTTGGATCTATCTTTTCTACCCTTTTGCCTGGCACAATGGGAAAGCTTGAACCTCCTGAAGGGTGCAGCTTCTTAGATGGACTTGAGGTTCGTGTTGCATTTGGGGGTGTTTGGAAACAGTCCTTATCAGAACTGAGTGGAGGTCAGCGATCTCTGCTTGCACTTTCCCTGATCTTGGCATTGCTTCTCTTCAAACCTGCCCCACTTTATATACTGGATGAGGTTGATGCGGCTCTTGATCTAAGCCACACGCAGAACATTGGAAGAATGATCAAAACGCATTTCCCCCACTCCCAGTTTATCGTGGTTTCGCTGAAAGAAGGCATGTTCAACAATGCTAACGTTCTGTTCCGAACGAAATTCGTGGACGGAGTTTCGACTGTTCAAAGGACTGTTGCAGCTAAGCACAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000793 GO:0000796 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044815

KEGG Pathways

Metabolic & Signaling

ko04111 map04111

Pfam Domains

Protein Families

SMC_N SMC_hinge

Protein Analysis

1176

Amino Acids

132.68

Weight (kDa)

6.75

Isoelectric Point (pI)

39.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SMC_N	PF02463	2 - 1161	1.6e-59	RecF/RecN/SMC N terminal domain
AAA_23	PF13476	8 - 218	6e-13	AAA domain
SMC_hinge	PF06470	518 - 638	4.9e-24	SMC proteins Flexible Hinge Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003832)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47460 AT3G47460 AT5G62410
fragaria_vesca	FvH4_7g25682 FvH4_7g25682 FvH4_7g25682 FvH4_7g25682 FvH4_7g25682 FvH4_7g27331 FvH4_7g27350 FvH4_7g27351 FvH4_7g27370 FvH4_7g27601 FvH4_7g27643 FvH4_7g27644 FvH4_7g27690 FvH4_7g28371 FvH4_7g28372 FvH4_7g28800
malus_domestica	MD01G1182300.v1.1 MD07G1252800.v1.1
prunus_persica	Prupe.2G276400_v2.0.a1
pyrus_communis	pycom01g19230 pycom07g22540
rosa_chinensis	RchiOBHm_Chr1g0374201
rosa_laevigata	RLG00000026756
rosa_multiflora	Rmu_co8310535.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283650
rosa_rugosa	Rorug01G0381300
rosa_samantha	Rh1AG392300 Rh1BG356300 Rh1CG369300 Rh1DG386800
rosa_wichuraiana	Rw1G034340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	157, 3319
AccB1I	GGYRCC	1 cut(s)	58
AccI	GTMKAC	2 cut(s)	198, 1656
AccII	CGCG	3 cut(s)	44, 174, 264
AclI	AACGTT	1 cut(s)	3456
AclWI	GGATC	8 cut(s)	690, 1443, 1523, 1536, 2243, 2870, 2883, 3127
AcsI	RAATTY	3 cut(s)	1856, 1926, 3473
AcuI	CTGAAG	8 cut(s)	1086, 1409, 1670, 2102, 2484, 2532, 2675, 3192
AfaI	GTAC	5 cut(s)	5, 1149, 1669, 2291, 2987
AfiI	CCNNNNNNNGG	3 cut(s)	2188, 3173, 3289
AflII	CTTAAG	1 cut(s)	2672
AjuI	GAANNNNNNNTTGG	2 cut(s)	1848, 1880
AloI	GAACNNNNNNTCC	2 cut(s)	3186, 3218
Alw21I	GWGCWC	1 cut(s)	195
Alw26I	GTCTC	6 cut(s)	241, 518, 732, 827, 2702, 2899
AlwI	GGATC	8 cut(s)	690, 1443, 1523, 1536, 2243, 2870, 2883, 3127
AlwNI	CAGNNNCTG	4 cut(s)	752, 1117, 1468, 2045
Ama87I	CYCGRG	3 cut(s)	62, 476, 2930
AoxI	GGCC	9 cut(s)	208, 228, 288, 537, 771, 1411, 1975, 2913, 2926
ApoI	RAATTY	3 cut(s)	1856, 1926, 3473
ArsI	GACNNNNNNTTYG	2 cut(s)	3186, 3218
Asp700I	GAANNNNTTC	3 cut(s)	717, 1489, 2126
AspA2I	CCTAGG	1 cut(s)	1757
AspLEI	GCGC	4 cut(s)	176, 266, 447, 709
AspS9I	GGNCC	6 cut(s)	208, 289, 379, 1411, 2134, 2914
AsuC2I	CCSGG	2 cut(s)	63, 64
AvaI	CYCGRG	3 cut(s)	62, 476, 2930
AvaII	GGWCC	2 cut(s)	379, 2134
AvrII	CCTAGG	1 cut(s)	1757
BaeGI	GKGCMC	2 cut(s)	63, 2608
BamHI	GGATCC	2 cut(s)	1528, 2875
BanI	GGYRCC	1 cut(s)	58
BanII	GRGCYC	1 cut(s)	195
BauI	CACGAG	1 cut(s)	2030
BbsI	GAAGAC	3 cut(s)	238, 554, 1023
Bbv12I	GWGCWC	1 cut(s)	195
BbvCI	CCTCAGC	1 cut(s)	1469
BccI	CCATC	4 cut(s)	140, 1036, 1411, 3185
BcgI	CGANNNNNNTGC	2 cut(s)	152, 186
BciVI	GTATCC	1 cut(s)	275
BclI	TGATCA	2 cut(s)	348, 3384
BcnI	CCSGG	2 cut(s)	63, 64
BcoDI	GTCTC	6 cut(s)	241, 518, 732, 827, 2702, 2899
BfaI	CTAG	6 cut(s)	372, 870, 1151, 1758, 1938, 1971
BfmI	CTRYAG	2 cut(s)	2265, 2909
BfrI	CTTAAG	1 cut(s)	2672
BfuAI	ACCTGC	2 cut(s)	157, 3319
BfuI	GTATCC	1 cut(s)	275
BglI	GCCNNNNNGGC	1 cut(s)	27
BglII	AGATCT	1 cut(s)	925
BlnI	CCTAGG	1 cut(s)	1757
BlpI	GCTNAGC	3 cut(s)	950, 2193, 3516
BmcAI	AGTACT	1 cut(s)	1669
Bme18I	GGWCC	2 cut(s)	379, 2134
BmeT110I	CYCGRG	3 cut(s)	62, 476, 2930
BmgT120I	GGNCC	6 cut(s)	208, 289, 379, 1411, 2134, 2914
BmiI	GGNNCC	3 cut(s)	60, 1530, 2877
BmrI	ACTGGG	2 cut(s)	1208, 3403
BmsI	GCATC	9 cut(s)	758, 901, 1195, 1318, 1885, 2074, 2215, 2504, 3331
BmuI	ACTGGG	2 cut(s)	1208, 3403
BpiI	GAAGAC	3 cut(s)	238, 554, 1023
BplI	GAGNNNNNCTC	2 cut(s)	2606, 2638
BpmI	CTGGAG	2 cut(s)	44, 170
Bpu10I	CCTNAGC	1 cut(s)	1469
Bpu1102I	GCTNAGC	3 cut(s)	950, 2193, 3516
BpuEI	CTTGAG	4 cut(s)	575, 2342, 2921, 3218
BpuMI	CCSGG	2 cut(s)	63, 64
Bsa29I	ATCGAT	1 cut(s)	585
BsaI	GGTCTC	1 cut(s)	518
BsaJI	CCNNGG	9 cut(s)	62, 225, 277, 453, 618, 783, 1141, 1757, 2182
Bsc4I	CCNNNNNNNGG	3 cut(s)	2188, 3173, 3289
Bse1I	ACTGG	5 cut(s)	1203, 1675, 1990, 3333, 3409
Bse3DI	GCAATG	4 cut(s)	662, 702, 2967, 3293
BseCI	ATCGAT	1 cut(s)	585
BseDI	CCNNGG	9 cut(s)	62, 225, 277, 453, 618, 783, 1141, 1757, 2182
BseGI	GGATG	5 cut(s)	120, 1480, 1753, 3067, 3337
BseLI	CCNNNNNNNGG	3 cut(s)	2188, 3173, 3289
BseMI	GCAATG	4 cut(s)	662, 702, 2967, 3293
BseMII	CTCAG	9 cut(s)	744, 941, 1124, 1437, 1460, 2184, 2604, 2814, 3239
BseNI	ACTGG	5 cut(s)	1203, 1675, 1990, 3333, 3409
BseRI	GAGGAG	1 cut(s)	1190
BseSI	GKGCMC	2 cut(s)	63, 2608
BseYI	CCCAGC	1 cut(s)	506
BsgI	GTGCAG	4 cut(s)	422, 765, 1481, 3199
Bsh1236I	CGCG	3 cut(s)	44, 174, 264
BshFI	GGCC	9 cut(s)	210, 230, 290, 539, 773, 1413, 1977, 2915, 2928
BshNI	GGYRCC	1 cut(s)	58
BshVI	ATCGAT	1 cut(s)	585
BsiHKAI	GWGCWC	1 cut(s)	195
BsiHKCI	CYCGRG	3 cut(s)	62, 476, 2930
BsiSI	CCGG	1 cut(s)	63
BslFI	GGGAC	3 cut(s)	258, 523, 2863
BslI	CCNNNNNNNGG	3 cut(s)	2188, 3173, 3289
BsmAI	GTCTC	6 cut(s)	241, 518, 732, 827, 2702, 2899
BsmBI	CGTCTC	2 cut(s)	241, 732
BsmFI	GGGAC	3 cut(s)	258, 523, 2863
BsmI	GAATGC	3 cut(s)	1804, 1852, 2667
BsnI	GGCC	9 cut(s)	210, 230, 290, 539, 773, 1413, 1977, 2915, 2928
Bso31I	GGTCTC	1 cut(s)	518
BsoBI	CYCGRG	3 cut(s)	62, 476, 2930
Bsp1286I	GDGCHC	3 cut(s)	63, 195, 2608
Bsp1407I	TGTACA	1 cut(s)	3
Bsp1720I	GCTNAGC	3 cut(s)	950, 2193, 3516
BspANI	GGCC	9 cut(s)	210, 230, 290, 539, 773, 1413, 1977, 2915, 2928
BspCNI	CTCAG	9 cut(s)	745, 942, 1123, 1438, 1461, 2185, 2605, 2815, 3240
BspDI	ATCGAT	1 cut(s)	585
BspFNI	CGCG	3 cut(s)	44, 174, 264
BspHI	TCATGA	1 cut(s)	2596
BspLI	GGNNCC	3 cut(s)	60, 1530, 2877
BspMAI	CTGCAG	2 cut(s)	2269, 2913
BspMI	ACCTGC	2 cut(s)	157, 3319
BspPI	GGATC	8 cut(s)	690, 1443, 1523, 1536, 2243, 2870, 2883, 3127
BspQI	GCTCTTC	1 cut(s)	3048
BspT107I	GGYRCC	1 cut(s)	58
BspTI	CTTAAG	1 cut(s)	2672
BspTNI	GGTCTC	1 cut(s)	518
BsrDI	GCAATG	4 cut(s)	662, 702, 2967, 3293
BsrGI	TGTACA	1 cut(s)	3
BsrI	ACTGG	5 cut(s)	1203, 1675, 1990, 3333, 3409
BssECI	CCNNGG	9 cut(s)	62, 225, 277, 453, 618, 783, 1141, 1757, 2182
BssSI	CACGAG	1 cut(s)	2030
BssT1I	CCWWGG	4 cut(s)	225, 453, 618, 1757
Bst2BI	CACGAG	1 cut(s)	2030
Bst6I	CTCTTC	6 cut(s)	492, 1702, 2121, 2289, 3048, 3308
BstAFI	CTTAAG	1 cut(s)	2672
BstAPI	GCANNNNNTGC	3 cut(s)	652, 1808, 2264
BstAUI	TGTACA	1 cut(s)	3
BstC8I	GCNNGC	7 cut(s)	615, 1156, 1809, 2037, 2808, 2913, 3273
BstENI	CCTNNNNNAGG	1 cut(s)	3171
BstF5I	GGATG	5 cut(s)	120, 1480, 1753, 3067, 3337
BstFNI	CGCG	3 cut(s)	44, 174, 264
BstHHI	GCGC	4 cut(s)	176, 266, 447, 709
BstMAI	GTCTC	6 cut(s)	241, 518, 732, 827, 2702, 2899
BstMWI	GCNNNNNNNGC	9 cut(s)	27, 652, 1214, 1808, 2203, 2212, 2264, 2576, 3268
BstNSI	RCATGY	2 cut(s)	2810, 3442
BstSFI	CTRYAG	2 cut(s)	2265, 2909
BstSLI	GKGCMC	2 cut(s)	63, 2608
BstUI	CGCG	3 cut(s)	44, 174, 264
BstV2I	GAAGAC	3 cut(s)	238, 554, 1023
BstX2I	RGATCY	4 cut(s)	925, 1528, 2875, 3119
BstXI	CCANNNNNNTGG	2 cut(s)	2663, 2924
BstYI	RGATCY	4 cut(s)	925, 1528, 2875, 3119
Bsu15I	ATCGAT	1 cut(s)	585
BsuI	GTATCC	1 cut(s)	275
BsuRI	GGCC	9 cut(s)	210, 230, 290, 539, 773, 1413, 1977, 2915, 2928
BsuTUI	ATCGAT	1 cut(s)	585
BtsCI	GGATG	5 cut(s)	120, 1480, 1753, 3067, 3337
BtsI	GCAGTG	1 cut(s)	1171
BtsIMutI	CAGTG	4 cut(s)	1115, 1171, 1385, 3080
BveI	ACCTGC	2 cut(s)	157, 3319
Cac8I	GCNNGC	7 cut(s)	615, 1156, 1809, 2037, 2808, 2913, 3273
CaiI	CAGNNNCTG	4 cut(s)	752, 1117, 1468, 2045
CciI	TCATGA	1 cut(s)	2596
CfoI	GCGC	4 cut(s)	176, 266, 447, 709
Cfr13I	GGNCC	6 cut(s)	208, 289, 379, 1411, 2134, 2914
Cfr9I	CCCGGG	1 cut(s)	62
ClaI	ATCGAT	1 cut(s)	585
CseI	GACGC	1 cut(s)	652
Csp6I	GTAC	5 cut(s)	4, 1148, 1668, 2290, 2986
CspCI	CAANNNNNGTGG	2 cut(s)	1147, 1182
CviAII	CATG	8 cut(s)	1021, 2062, 2131, 2438, 2597, 2632, 2807, 3439
CviQI	GTAC	5 cut(s)	4, 1148, 1668, 2290, 2986
Eam1104I	CTCTTC	6 cut(s)	492, 1702, 2121, 2289, 3048, 3308
EarI	CTCTTC	6 cut(s)	492, 1702, 2121, 2289, 3048, 3308
Ecl136II	GAGCTC	1 cut(s)	193
Eco130I	CCWWGG	4 cut(s)	225, 453, 618, 1757
Eco24I	GRGCYC	1 cut(s)	195
Eco31I	GGTCTC	1 cut(s)	518
Eco32I	GATATC	1 cut(s)	1960
Eco47I	GGWCC	2 cut(s)	379, 2134
Eco53kI	GAGCTC	1 cut(s)	193
Eco57I	CTGAAG	8 cut(s)	1086, 1409, 1670, 2102, 2484, 2532, 2675, 3192
Eco88I	CYCGRG	3 cut(s)	62, 476, 2930
EcoICRI	GAGCTC	1 cut(s)	193
EcoNI	CCTNNNNNAGG	1 cut(s)	3171
EcoRV	GATATC	1 cut(s)	1960
EcoT14I	CCWWGG	4 cut(s)	225, 453, 618, 1757
EcoT22I	ATGCAT	1 cut(s)	2067
EcoT38I	GRGCYC	1 cut(s)	195
ErhI	CCWWGG	4 cut(s)	225, 453, 618, 1757
Esp3I	CGTCTC	2 cut(s)	241, 732
FaeI	CATG	8 cut(s)	1024, 2065, 2134, 2441, 2600, 2635, 2810, 3442
FalI	AAGNNNNNCTT	2 cut(s)	2442, 2474
FaqI	GGGAC	3 cut(s)	258, 523, 2863
FatI	CATG	8 cut(s)	1020, 2061, 2130, 2437, 2596, 2631, 2806, 3438
FauI	CCCGC	2 cut(s)	429, 2865
FbaI	TGATCA	2 cut(s)	348, 3384
FblI	GTMKAC	2 cut(s)	198, 1656
FokI	GGATG	5 cut(s)	107, 1487, 1740, 3074, 3344
FriOI	GRGCYC	1 cut(s)	195
FspBI	CTAG	6 cut(s)	372, 870, 1151, 1758, 1938, 1971
GlaI	GCGC	4 cut(s)	175, 265, 446, 708
GsaI	CCCAGC	1 cut(s)	510
GsuI	CTGGAG	2 cut(s)	44, 170
HaeIII	GGCC	9 cut(s)	210, 230, 290, 539, 773, 1413, 1977, 2915, 2928
HapII	CCGG	1 cut(s)	63
HgaI	GACGC	1 cut(s)	652
HhaI	GCGC	4 cut(s)	176, 266, 447, 709
Hin1II	CATG	8 cut(s)	1024, 2065, 2134, 2441, 2600, 2635, 2810, 3442
Hin6I	GCGC	4 cut(s)	174, 264, 445, 707
HinP1I	GCGC	4 cut(s)	174, 264, 445, 707
HincII	GTYRAC	3 cut(s)	415, 852, 1006
HindII	GTYRAC	3 cut(s)	415, 852, 1006
HindIII	AAGCTT	4 cut(s)	590, 2213, 2456, 3158
HinfI	GANTC	6 cut(s)	128, 219, 481, 2042, 2425, 2651
HpaI	GTTAAC	2 cut(s)	415, 1006
HpaII	CCGG	1 cut(s)	63
Hpy166II	GTNNAC	9 cut(s)	199, 415, 852, 1006, 1148, 1343, 1657, 2699, 3481
Hpy8I	GTNNAC	9 cut(s)	199, 415, 852, 1006, 1148, 1343, 1657, 2699, 3481
Hpy99I	CGWCG	2 cut(s)	49, 1606
HpyCH4IV	ACGT	1 cut(s)	3456
HpyF10VI	GCNNNNNNNGC	9 cut(s)	27, 652, 1214, 1808, 2203, 2212, 2264, 2576, 3268
HpySE526I	ACGT	1 cut(s)	3456
Hsp92II	CATG	8 cut(s)	1024, 2065, 2134, 2441, 2600, 2635, 2810, 3442
HspAI	GCGC	4 cut(s)	174, 264, 445, 707
Ksp22I	TGATCA	2 cut(s)	348, 3384
KspAI	GTTAAC	2 cut(s)	415, 1006
LguI	GCTCTTC	1 cut(s)	3048
LmnI	GCTCC	4 cut(s)	190, 1073, 2677, 2761
LweI	GCATC	9 cut(s)	758, 901, 1195, 1318, 1885, 2074, 2215, 2504, 3331
MaeI	CTAG	6 cut(s)	372, 870, 1151, 1758, 1938, 1971
MaeII	ACGT	1 cut(s)	3456
MaeIII	GTNAC	6 cut(s)	307, 1621, 1711, 1942, 2311, 3088
MflI	RGATCY	4 cut(s)	925, 1528, 2875, 3119
MhlI	GDGCHC	3 cut(s)	63, 195, 2608
MlyI	GAGTC	2 cut(s)	122, 2051
MmeI	TCCRAC	2 cut(s)	141, 738
Mph1103I	ATGCAT	1 cut(s)	2067
MroXI	GAANNNNTTC	3 cut(s)	717, 1489, 2126
MslI	CAYNNNNRTG	3 cut(s)	2442, 2601, 2636
MspA1I	CMGCKG	2 cut(s)	1079, 1249
MspCI	CTTAAG	1 cut(s)	2672
MspI	CCGG	1 cut(s)	63
Mva1269I	GAATGC	3 cut(s)	1804, 1852, 2667
MvnI	CGCG	3 cut(s)	44, 174, 264
MwoI	GCNNNNNNNGC	9 cut(s)	27, 652, 1214, 1808, 2203, 2212, 2264, 2576, 3268
NciI	CCSGG	2 cut(s)	63, 64
NlaIII	CATG	8 cut(s)	1024, 2065, 2134, 2441, 2600, 2635, 2810, 3442
NlaIV	GGNNCC	3 cut(s)	60, 1530, 2877
NmuCI	GTSAC	2 cut(s)	307, 1942
NsiI	ATGCAT	1 cut(s)	2067
NspI	RCATGY	2 cut(s)	2810, 3442
PaeI	GCATGC	1 cut(s)	2810
PaeR7I	CTCGAG	1 cut(s)	2930
PagI	TCATGA	1 cut(s)	2596
PciSI	GCTCTTC	1 cut(s)	3048
PctI	GAATGC	3 cut(s)	1804, 1852, 2667
PdmI	GAANNNNTTC	3 cut(s)	717, 1489, 2126
PfeI	GAWTC	4 cut(s)	219, 481, 2425, 2651
PfoI	TCCNGGA	1 cut(s)	186
PleI	GAGTC	2 cut(s)	122, 2050
PpsI	GAGTC	2 cut(s)	122, 2050
Psp124BI	GAGCTC	1 cut(s)	195
Psp1406I	AACGTT	1 cut(s)	3456
PspFI	CCCAGC	1 cut(s)	506
PspN4I	GGNNCC	3 cut(s)	60, 1530, 2877
PspPI	GGNCC	6 cut(s)	208, 289, 379, 1411, 2134, 2914
PstI	CTGCAG	2 cut(s)	2269, 2913
PstNI	CAGNNNCTG	4 cut(s)	752, 1117, 1468, 2045
PsuI	RGATCY	4 cut(s)	925, 1528, 2875, 3119
PvuII	CAGCTG	2 cut(s)	1079, 1249
RsaI	GTAC	5 cut(s)	5, 1149, 1669, 2291, 2987
RsaNI	GTAC	5 cut(s)	4, 1148, 1668, 2290, 2986
RseI	CAYNNNNRTG	3 cut(s)	2442, 2601, 2636
SacI	GAGCTC	1 cut(s)	195
SapI	GCTCTTC	1 cut(s)	3048
Sau96I	GGNCC	6 cut(s)	208, 289, 379, 1411, 2134, 2914
ScaI	AGTACT	1 cut(s)	1669
SchI	GAGTC	2 cut(s)	122, 2051
SduI	GDGCHC	3 cut(s)	63, 195, 2608
SfaNI	GCATC	9 cut(s)	758, 901, 1195, 1318, 1885, 2074, 2215, 2504, 3331
SfcI	CTRYAG	2 cut(s)	2265, 2909
Sfr274I	CTCGAG	1 cut(s)	2930
SinI	GGWCC	2 cut(s)	379, 2134
SlaI	CTCGAG	1 cut(s)	2930
SmaI	CCCGGG	1 cut(s)	64
SmiMI	CAYNNNNRTG	3 cut(s)	2442, 2601, 2636
SmlI	CTYRAG	6 cut(s)	554, 2321, 2672, 2900, 2930, 3197
SmoI	CTYRAG	6 cut(s)	554, 2321, 2672, 2900, 2930, 3197
SphI	GCATGC	1 cut(s)	2810
SspI	AATATT	2 cut(s)	1012, 2593
SspMI	CTAG	6 cut(s)	372, 870, 1151, 1758, 1938, 1971
SstI	GAGCTC	1 cut(s)	195
StyI	CCWWGG	4 cut(s)	225, 453, 618, 1757
TaiI	ACGT	1 cut(s)	3459
TaqII	GACCGA	1 cut(s)	2096
TatI	WGTACW	4 cut(s)	3, 1147, 1667, 2985
TauI	GCSGC	4 cut(s)	179, 542, 1998, 3347
TfiI	GAWTC	4 cut(s)	219, 481, 2425, 2651
TscAI	CASTG	4 cut(s)	1122, 1171, 1392, 3087
TseFI	GTSAC	2 cut(s)	307, 1942
Tsp45I	GTSAC	2 cut(s)	307, 1942
TspGWI	ACGGA	4 cut(s)	388, 1676, 2774, 3498
TspMI	CCCGGG	1 cut(s)	62
TspRI	CASTG	4 cut(s)	1122, 1171, 1392, 3087
Vha464I	CTTAAG	1 cut(s)	2672
VpaK11BI	GGWCC	2 cut(s)	379, 2134
XagI	CCTNNNNNAGG	1 cut(s)	3171
XapI	RAATTY	3 cut(s)	1856, 1926, 3473
XceI	RCATGY	2 cut(s)	2810, 3442
XhoI	CTCGAG	1 cut(s)	2930
XmaI	CCCGGG	1 cut(s)	62
XmaJI	CCTAGG	1 cut(s)	1757
XmiI	GTMKAC	2 cut(s)	198, 1656
XmnI	GAANNNNTTC	3 cut(s)	717, 1489, 2126
XspI	CTAG	6 cut(s)	372, 870, 1151, 1758, 1938, 1971
ZrmI	AGTACT	1 cut(s)	1669
Zsp2I	ATGCAT	1 cut(s)	2067

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.