Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0003832

Physical Location & Seq

Genomic Coordinates Reverse (-)

60648686 .. 60655768

Gene Structure 7083 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG386800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3528 bp

ATGTACATCAAGGAGGTGACCTTAGAAGGGTTCAAGTCGTACGCGACGCGGACGGTGGTGCCGGGATTCGACCCGTTTTTCAACGCCATAACCGGGCTAAACGGGTCGGGTAAGTCCAACATTCTCGATTCGATTTGCTTCGTTCTCGGCATCACCAACTTACAGCAAGTCCGGGCGGCCAATCTGCAAGAGCTAGTGTACAAGCAAGGCCAGGCCGGAATTACTAAGGCCACCGTCTCCATCGTCTTCGACAATTCGGACCGGGCTCGCAGTCCTCTCGGGTACGAGGCCCATTCCGAACTCACCGTCACCCGGCAGATTGTGGTTGGTGGAAGGAACAAGTACTTGATCAATGGGAAACTTGCGCAGCCAAGTCAGGTCCAGAATCTTTTTCATTCGGTGCAGCTCAATGTGAATAATCCGCATTTTCTCATTATGCAAGGCCGGATTACCAAGGTTTTGAATATGAAACCGCCTGAGATTCTGTCTATGCTTGAAGAGGCTGCTGGGACGAGAATGTATGAAACCAAAAAGGAAGCCGCTCTAAAAACACTTGAGAAGAAGCAGAGTAAAGTTGATGAGATAAATAAGCTTCTTGACCACGAGATACTGCCTGCCTTGGACAAGTTGAGGAGAGAAAGGACACAATATATGCAATGGGCTAATGGCAATGCTGACTTGGATCGGCTTAAAAGGTTCTGCATTGCTTATGAATATGTTCAAGCAGAGAGGATTAGAGACACTGCAGTTTGTGAGGTGGAACAAGTGAAGGCCAAAATTTCTGAGGTTGACGATGATACAAGAAAGATGCAGGCAGCAATACAGGAAATGGAGACACAAGTGTCAAAATTGACTGCTGAAAAGGAAGCTAGTATGGGTGGAGAAGTAAAAGTTTTGTCCACCCAAGTAGATAAGCTTTCTCAAGATGTAGTAAGGGAAGTGTCTATACTGGATAATATGAAGGACAATCTTGATACTGAAAATGAAAATGCTAAAAAGATTGCTTGCAACATTGAAGACATGAAACAGTCTCTAAAAGATAGGGACTGTGCTATCAGAAATGCTGAAGAAGGAGCTGCTGATTTGAAAAGGAGAGCGGAGGAACTTTCTCATAGTCTGAATGAGTATGAGACGCAGTACCAGGGTGTAATCGCTGGCAAGAGTAGCGGAAATGAGGAGAAATGTCTTGAAGATCAATTGGGTGATGCCAAAAGAGCTGTAGGGAGTGCTGAAACAGAATTGGAACAGTTGAAAACAAAAATTCGTCACTGTGAAAAGGAGTTGAAAGAGAAAAATTCTCAACTAATGTCAAAGCGTGAAGAAGCTGTTGCTGTAGAGAGTGAGCTGAAAGCTAGGAAAACAGATGTGGAAAATGTTAAAGTGGCACTGGAGTCTCTTCCATATAAGGAGGGCCAGATAGAAGCATTGCAAAAGGACCGGTCATATGAGTTGGAGTGTGTGCAGAAGTTGAAGGATGAAATGCGTAATCTTTCAGGACACTTGGGAAATGTTGACTTTTCTTATAGAGATCCTGTGAATAATTTTGCTAGGTCTAAGGTCAAGGGTGTAGTTGCAAAACTTATCAAAGTGAAGGATAGCTCCACAATGACTGCCTTAGAGGTCACTGCTGGTGGAAAGTTGTTTAACGTTGTTGTAGACACAGAAAGTACCGGAAAACTACTTCTTCAGAATGGTAATCTTCGAAGGAGAGTAACAATCATACCCTTGAACAAAATTCAAGCTCACACAGTTCCTCCTAGGGTTCAACATGCTGCTGCTAAATTGGTTGGCAAGGGAAACGCAGAACTGGCACTTTCTTTGGTTGGCTATGATGAGCAACTGAGGAGTGCTATGGAATATGTCTTCGGTTCAACCTTTGTGTGCAAAACTATTGATGCTGCAAAGGAGGTTGCGTTTAACAGGGACGTTCGCACCCCAAGTGTCACTCTTGAAGGTGATATCTTTCAGCCCAGTGGTCTTTTGACTGGTGGAAGCCGCAAGGGTGGGGGTGATCTGTTAAGGCAGCTTCATGAGCTGGCTGAAGCTGAGCTAAAACTATCCGAGCACCAGAAAAAGTTGACTGAAATTGAAGCTAAGATTAGAGAGATTCAGCCTCTTCACAAGAAGTTCATGGAGCTTAAAGCACAGTTAGAACTCAAATCATATGACCTTAAATTATTTCAGGGCAGGGCTGAACAAAATGAGCATCATAAGCTTGGTGAATTAGTAAAGAGGATTGAGCAAGAGCTACAAGAAGCAAATTCTGCAGCCAAAGAAAAGAAACTTCTGCATGAAGATTGTGTGAATAAAGTGTTGTTTCTTGAGAAATCAATCAAAGAGCATGACAACAGTCGGGAGGGAAGGCTGAAAGATTTGGAAAAGAAGATCAAAGAGACAAAAGCTCAACTCCAGTCAGCTTCAAAGGATCTCAAGGGACATGAAAATGAAAAGGAGAAGCTTATTATGGAAAAGGATGTTGTTTTAAAAGAGCTTGCATCTTTGGAGACTCAGTTGTCTTCGTTGAGAGCACAAATTGATGGTCTCACTTCAGAAGTAGAAGAACAGAAAGAAAAGGTAAATTCTACAAGAAATAAGCATGATGATTCAAAATCCCAGCTTGATTCAATTCGTATGAAGATGAAGGAGTGTGATTCCCAAATTAATGGCATTGAATCTGATCAGCAAAAACTTCAAGACAAACTTCGTGAGACAAATCTTGAACGGAAAAGGATGGAAAATGAGGTTAAAAGAATGGAAATGGAACAGAAAGACTGCTCTACGAAAGTAGACAAGTTGATAAAGAGGCATGCCTGGATAACATCTGAGAAACAGCTATTTGGAAAAACTGGGACTGATTATGATTTTGCCATGCGGGATCCTAGCAATGCAAGGGAAGAACTAGAGAAGCTGCAGGCTCAACAATCTGGCCTTGAGAAAAGGGTGAATAAGAAAGTTATGGCAATGTTTGAGAAAGCAGAAGACGAGTACAATGACCTAATATCTAAGAAGAACATCATTGAGAATGACAAGTCTAAGATCAAGATGGTGATTGAAGAGCTGGATGAGAAAAAGAAGGAAACATTGAACGTTACTTGGGTCAAAGTTAACAAAGACTTTGGGTCTATCTTTTCTACCCTTTTACCCGGCACGATGGGAAAGCTTGAACCTCCTGAAGGGTGCAGCTTCCTAGATGGCCTTGAGGTGCGTGTTGCATTTGGTGGTGTTTGGAAACAGTCCTTGTCAGAACTGAGTGGAGGTCAGCGATCCCTGCTAGCACTTTCTCTGATCTTGGCATTGCTTCTATTCAAGCCTGCTCCTCTGTATATACTGGATGAGGTTGATGCAGCTCTTGATCTAAGTCACACCCAGAATATTGGAAGAATGATCAAAAGTCATTTCCCACACTCCCAGTTCATAGTGGTTTCACTGAAAGAAGGCATGTTCAATAATGCTAATGTCCTTTTCCGGACAAAATTTGTGGATGGAGTTTCTACTGTTCACAGGACCGTTGCTAAGCAAAACAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000793 GO:0000796 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044815

KEGG Pathways

Metabolic & Signaling

ko04111 map04111

Pfam Domains

Protein Families

SMC_N SMC_hinge

Protein Analysis

1175

Amino Acids

132.71

Weight (kDa)

7.77

Isoelectric Point (pI)

37.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SMC_N	PF02463	2 - 456	7.8e-35	RecF/RecN/SMC N terminal domain
AAA_23	PF13476	6 - 213	7e-06	AAA domain
SMC_hinge	PF06470	518 - 638	2.2e-23	SMC proteins Flexible Hinge Domain
SMC_N	PF02463	858 - 1161	2.9e-29	RecF/RecN/SMC N terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003832)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47460 AT3G47460 AT5G62410
fragaria_vesca	FvH4_7g25682 FvH4_7g25682 FvH4_7g25682 FvH4_7g25682 FvH4_7g25682 FvH4_7g27331 FvH4_7g27350 FvH4_7g27351 FvH4_7g27370 FvH4_7g27601 FvH4_7g27643 FvH4_7g27644 FvH4_7g27690 FvH4_7g28371 FvH4_7g28372 FvH4_7g28800
malus_domestica	MD01G1182300.v1.1 MD07G1252800.v1.1
prunus_persica	Prupe.2G276400_v2.0.a1
pyrus_communis	pycom01g19230 pycom07g22540
rosa_chinensis	RchiOBHm_Chr1g0374201
rosa_laevigata	RLG00000026756
rosa_multiflora	Rmu_co8310535.1_g000001
rosa_roxburghii	Rroxscaffold_4G00283650
rosa_rugosa	Rorug01G0381300
rosa_samantha	Rh1AG392300 Rh1BG356300 Rh1CG369300 Rh1DG386800
rosa_wichuraiana	Rw1G034340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	841, 2512
Acc16I	TGCGCA	1 cut(s)	366
AccB1I	GGYRCC	1 cut(s)	58
AccBSI	CCGCTC	2 cut(s)	542, 1097
AccI	GTMKAC	2 cut(s)	1656, 2787
AccII	CGCG	2 cut(s)	44, 49
AccIII	TCCGGA	1 cut(s)	3465
AciI	CCGC	9 cut(s)	49, 176, 422, 473, 540, 1097, 1167, 1996, 2872
AclI	AACGTT	2 cut(s)	1647, 3087
AclWI	GGATC	6 cut(s)	690, 1523, 2433, 2870, 2883, 3258
AcoI	YGGCCR	1 cut(s)	177
AcsI	RAATTY	7 cut(s)	777, 1260, 1294, 1734, 2260, 2578, 3473
AcuI	CTGAAG	5 cut(s)	1086, 1670, 2061, 2532, 3192
AfaI	GTAC	8 cut(s)	5, 41, 200, 284, 344, 1139, 1669, 2987
AfiI	CCNNNNNNNGG	4 cut(s)	27, 93, 312, 3173
AgeI	ACCGGT	1 cut(s)	1437
AhdI	GACNNNNNGTC	1 cut(s)	3118
AjnI	CCWGG	3 cut(s)	210, 1140, 2810
Alw21I	GWGCWC	2 cut(s)	2067, 2530
AlwI	GGATC	6 cut(s)	690, 1523, 2433, 2870, 2883, 3258
Ama87I	CYCGRG	1 cut(s)	278
Aor13HI	TCCGGA	1 cut(s)	3465
ApoI	RAATTY	7 cut(s)	777, 1260, 1294, 1734, 2260, 2578, 3473
ArsI	GACNNNNNNTTYG	4 cut(s)	291, 323, 2524, 2556
AseI	ATTAAT	1 cut(s)	2661
AsiGI	ACCGGT	1 cut(s)	1437
Asp700I	GAANNNNTTC	1 cut(s)	717
AspA2I	CCTAGG	1 cut(s)	1757
AspLEI	GCGC	1 cut(s)	367
AspS9I	GGNCC	6 cut(s)	259, 289, 379, 1411, 1435, 3504
AsuC2I	CCSGG	6 cut(s)	63, 94, 173, 263, 313, 3144
AsuII	TTCGAA	1 cut(s)	1702
AsuNHI	GCTAGC	1 cut(s)	3271
AvaI	CYCGRG	1 cut(s)	278
AvaII	GGWCC	4 cut(s)	259, 379, 1435, 3504
AvrII	CCTAGG	1 cut(s)	1757
BamHI	GGATCC	1 cut(s)	2875
BanI	GGYRCC	1 cut(s)	58
BanII	GRGCYC	1 cut(s)	268
BarI	GAAGNNNNNNTAC	2 cut(s)	930, 962
BauI	CACGAG	1 cut(s)	602
BbsI	GAAGAC	5 cut(s)	238, 1023, 1855, 2508, 2985
Bbv12I	GWGCWC	2 cut(s)	2067, 2530
BccI	CCATC	7 cut(s)	248, 2531, 2725, 3037, 3145, 3185, 3476
BcgI	CGANNNNNNTGC	2 cut(s)	259, 293
BciT130I	CCWGG	3 cut(s)	212, 1142, 2812
BclI	TGATCA	3 cut(s)	348, 2677, 3384
BcnI	CCSGG	6 cut(s)	63, 94, 173, 263, 313, 3144
BfaI	CTAG	9 cut(s)	194, 870, 1353, 1548, 1758, 2880, 2900, 3188, 3272
BfmI	CTRYAG	5 cut(s)	744, 1218, 1332, 2265, 2909
BlnI	CCTAGG	1 cut(s)	1757
BlpI	GCTNAGC	2 cut(s)	2046, 3513
BmcAI	AGTACT	1 cut(s)	344
Bme1390I	CCNGG	9 cut(s)	63, 94, 173, 212, 263, 313, 1142, 2812, 3144
Bme18I	GGWCC	4 cut(s)	259, 379, 1435, 3504
BmeRI	GACNNNNNGTC	1 cut(s)	3118
BmeT110I	CYCGRG	1 cut(s)	278
BmgT120I	GGNCC	6 cut(s)	259, 289, 379, 1411, 1435, 3504
BmiI	GGNNCC	2 cut(s)	60, 2877
BmrFI	CCNGG	9 cut(s)	63, 94, 173, 212, 263, 313, 1142, 2812, 3144
BmrI	ACTGGG	3 cut(s)	1965, 2856, 3403
BmsI	GCATC	7 cut(s)	159, 798, 1195, 1885, 2215, 2504, 3331
BmtI	GCTAGC	1 cut(s)	3275
BmuI	ACTGGG	3 cut(s)	1965, 2856, 3403
BoxI	GACNNNNGTC	1 cut(s)	2349
BpiI	GAAGAC	5 cut(s)	238, 1023, 1855, 2508, 2985
BpmI	CTGGAG	2 cut(s)	1409, 2393
Bpu1102I	GCTNAGC	2 cut(s)	2046, 3513
Bpu14I	TTCGAA	1 cut(s)	1702
BpuEI	CTTGAG	6 cut(s)	575, 906, 2342, 2414, 2951, 3218
BpuMI	CCSGG	6 cut(s)	63, 94, 173, 263, 313, 3144
BsaI	GGTCTC	1 cut(s)	2546
BsaJI	CCNNGG	4 cut(s)	453, 618, 1141, 1757
BsaWI	WCCGGW	3 cut(s)	1437, 1670, 3465
BsaXI	ACNNNNNCTCC	6 cut(s)	1738, 1768, 1898, 1928, 2495, 2525
Bsc4I	CCNNNNNNNGG	4 cut(s)	27, 93, 312, 3173
Bse118I	RCCGGY	1 cut(s)	1437
Bse1I	ACTGG	9 cut(s)	954, 1392, 1812, 1971, 1990, 2410, 2851, 3333, 3409
Bse3DI	GCAATG	7 cut(s)	662, 676, 702, 1424, 2890, 2967, 3293
BseAI	TCCGGA	1 cut(s)	3465
BseBI	CCWGG	3 cut(s)	212, 1142, 2812
BseDI	CCNNGG	4 cut(s)	453, 618, 1141, 1757
BseGI	GGATG	6 cut(s)	1480, 2479, 2736, 3067, 3337, 3487
BseLI	CCNNNNNNNGG	4 cut(s)	27, 93, 312, 3173
BseMI	GCAATG	7 cut(s)	662, 676, 702, 1424, 2890, 2967, 3293
BseMII	CTCAG	7 cut(s)	468, 774, 1832, 2037, 2522, 2814, 3239
BseNI	ACTGG	9 cut(s)	954, 1392, 1812, 1971, 1990, 2410, 2851, 3333, 3409
BseRI	GAGGAG	4 cut(s)	646, 1190, 1858, 3306
BseYI	CCCAGC	2 cut(s)	506, 2613
BsgI	GTGCAG	3 cut(s)	422, 1481, 3199
Bsh1236I	CGCG	2 cut(s)	44, 49
BshNI	GGYRCC	1 cut(s)	58
BshTI	ACCGGT	1 cut(s)	1437
BsiHKAI	GWGCWC	2 cut(s)	2067, 2530
BsiHKCI	CYCGRG	1 cut(s)	278
BsiWI	CGTACG	1 cut(s)	39
BslFI	GGGAC	5 cut(s)	523, 1058, 1937, 2448, 2863
BslI	CCNNNNNNNGG	4 cut(s)	27, 93, 312, 3173
BsmBI	CGTCTC	2 cut(s)	241, 1124
BsmFI	GGGAC	5 cut(s)	523, 1058, 1937, 2448, 2863
Bso31I	GGTCTC	1 cut(s)	2546
BsoBI	CYCGRG	1 cut(s)	278
Bsp119I	TTCGAA	1 cut(s)	1702
Bsp1286I	GDGCHC	3 cut(s)	268, 2067, 2530
Bsp13I	TCCGGA	1 cut(s)	3465
Bsp1407I	TGTACA	2 cut(s)	3, 198
Bsp1720I	GCTNAGC	2 cut(s)	2046, 3513
BspACI	CCGC	9 cut(s)	49, 176, 422, 473, 540, 1097, 1167, 1996, 2872
BspCNI	CTCAG	7 cut(s)	469, 775, 1833, 2038, 2521, 2815, 3240
BspEI	TCCGGA	1 cut(s)	3465
BspFNI	CGCG	2 cut(s)	44, 49
BspHI	TCATGA	1 cut(s)	2029
BspLI	GGNNCC	2 cut(s)	60, 2877
BspMAI	CTGCAG	3 cut(s)	748, 2269, 2913
BspOI	GCTAGC	1 cut(s)	3275
BspPI	GGATC	6 cut(s)	690, 1523, 2433, 2870, 2883, 3258
BspQI	GCTCTTC	1 cut(s)	3048
BspT104I	TTCGAA	1 cut(s)	1702
BspT107I	GGYRCC	1 cut(s)	58
BspTNI	GGTCTC	1 cut(s)	2546
BsrBI	CCGCTC	2 cut(s)	542, 1097
BsrDI	GCAATG	7 cut(s)	662, 676, 702, 1424, 2890, 2967, 3293
BsrFI	RCCGGY	1 cut(s)	1437
BsrGI	TGTACA	2 cut(s)	3, 198
BsrI	ACTGG	9 cut(s)	954, 1392, 1812, 1971, 1990, 2410, 2851, 3333, 3409
BssAI	RCCGGY	1 cut(s)	1437
BssECI	CCNNGG	4 cut(s)	453, 618, 1141, 1757
BssSI	CACGAG	1 cut(s)	602
BssT1I	CCWWGG	3 cut(s)	453, 618, 1757
Bst2BI	CACGAG	1 cut(s)	602
Bst2UI	CCWGG	3 cut(s)	212, 1142, 2812
Bst6I	CTCTTC	4 cut(s)	492, 1401, 2121, 3048
BstAPI	GCANNNNNTGC	1 cut(s)	2264
BstAUI	TGTACA	2 cut(s)	3, 198
BstBI	TTCGAA	1 cut(s)	1702
BstEII	GGTNACC	1 cut(s)	16
BstENI	CCTNNNNNAGG	1 cut(s)	3171
BstF5I	GGATG	6 cut(s)	1480, 2479, 2736, 3067, 3337, 3487
BstFNI	CGCG	2 cut(s)	44, 49
BstHHI	GCGC	1 cut(s)	367
BstMWI	GCNNNNNNNGC	7 cut(s)	1164, 1214, 1808, 2032, 2212, 2264, 3268
BstNI	CCWGG	3 cut(s)	212, 1142, 2812
BstNSI	RCATGY	3 cut(s)	1772, 2810, 3442
BstPAI	GACNNNNGTC	1 cut(s)	2349
BstPI	GGTNACC	1 cut(s)	16
BstSCI	CCNGG	9 cut(s)	61, 92, 171, 210, 261, 311, 1140, 2810, 3142
BstSFI	CTRYAG	5 cut(s)	744, 1218, 1332, 2265, 2909
BstUI	CGCG	2 cut(s)	44, 49
BstV2I	GAAGAC	5 cut(s)	238, 1023, 1855, 2508, 2985
BstX2I	RGATCY	3 cut(s)	1528, 2425, 2875
BstXI	CCANNNNNNTGG	2 cut(s)	2663, 3374
BstYI	RGATCY	3 cut(s)	1528, 2425, 2875
BtsCI	GGATG	6 cut(s)	1480, 2479, 2736, 3067, 3337, 3487
BtsI	GCAGTG	2 cut(s)	741, 1623
BtsIMutI	CAGTG	6 cut(s)	741, 1267, 1385, 1623, 1978, 3425
CciI	TCATGA	1 cut(s)	2029
CfoI	GCGC	1 cut(s)	367
Cfr10I	RCCGGY	1 cut(s)	1437
Cfr13I	GGNCC	6 cut(s)	259, 289, 379, 1411, 1435, 3504
CpoI	CGGWCCG	1 cut(s)	259
CseI	GACGC	2 cut(s)	55, 1141
Csp6I	GTAC	8 cut(s)	4, 40, 199, 283, 343, 1138, 1668, 2986
CspAI	ACCGGT	1 cut(s)	1437
CspCI	CAANNNNNGTGG	2 cut(s)	3459, 3494
CspI	CGGWCCG	1 cut(s)	259
CviQI	GTAC	8 cut(s)	4, 40, 199, 283, 343, 1138, 1668, 2986
DraI	TTTAAA	1 cut(s)	2484
DrdI	GACNNNNNNGTC	2 cut(s)	841, 2512
DriI	GACNNNNNGTC	1 cut(s)	3118
DseDI	GACNNNNNNGTC	2 cut(s)	841, 2512
EaeI	YGGCCR	1 cut(s)	177
Eam1104I	CTCTTC	4 cut(s)	492, 1401, 2121, 3048
Eam1105I	GACNNNNNGTC	1 cut(s)	3118
EarI	CTCTTC	4 cut(s)	492, 1401, 2121, 3048
Eco130I	CCWWGG	3 cut(s)	453, 618, 1757
Eco24I	GRGCYC	1 cut(s)	268
Eco31I	GGTCTC	1 cut(s)	2546
Eco32I	GATATC	1 cut(s)	1960
Eco47I	GGWCC	4 cut(s)	259, 379, 1435, 3504
Eco57I	CTGAAG	5 cut(s)	1086, 1670, 2061, 2532, 3192
Eco88I	CYCGRG	1 cut(s)	278
Eco91I	GGTNACC	1 cut(s)	16
EcoNI	CCTNNNNNAGG	1 cut(s)	3171
EcoO65I	GGTNACC	1 cut(s)	16
EcoRII	CCWGG	3 cut(s)	210, 1140, 2810
EcoRV	GATATC	1 cut(s)	1960
EcoT14I	CCWWGG	3 cut(s)	453, 618, 1757
EcoT38I	GRGCYC	1 cut(s)	268
ErhI	CCWWGG	3 cut(s)	453, 618, 1757
Esp3I	CGTCTC	2 cut(s)	241, 1124
FalI	AAGNNNNNCTT	2 cut(s)	2685, 2717
FaqI	GGGAC	5 cut(s)	523, 1058, 1937, 2448, 2863
FauI	CCCGC	1 cut(s)	2865
FauNDI	CATATG	2 cut(s)	1444, 2164
FbaI	TGATCA	3 cut(s)	348, 2677, 3384
FblI	GTMKAC	2 cut(s)	1656, 2787
FokI	GGATG	6 cut(s)	1487, 2486, 2743, 3074, 3344, 3494
FriOI	GRGCYC	1 cut(s)	268
FspBI	CTAG	9 cut(s)	194, 870, 1353, 1548, 1758, 2880, 2900, 3188, 3272
FspI	TGCGCA	1 cut(s)	366
GlaI	GCGC	1 cut(s)	366
GsaI	CCCAGC	2 cut(s)	510, 2617
GsuI	CTGGAG	2 cut(s)	1409, 2393
HgaI	GACGC	2 cut(s)	55, 1141
HhaI	GCGC	1 cut(s)	367
Hin6I	GCGC	1 cut(s)	365
HinP1I	GCGC	1 cut(s)	365
HincII	GTYRAC	4 cut(s)	790, 1513, 2079, 3106
HindII	GTYRAC	4 cut(s)	790, 1513, 2079, 3106
HindIII	AAGCTT	5 cut(s)	590, 914, 2213, 2456, 3158
HpaI	GTTAAC	1 cut(s)	3106
Hpy166II	GTNNAC	9 cut(s)	199, 790, 900, 1513, 1657, 2079, 2788, 3106, 3499
Hpy8I	GTNNAC	9 cut(s)	199, 790, 900, 1513, 1657, 2079, 2788, 3106, 3499
Hpy99I	CGWCG	1 cut(s)	49
HpyCH4IV	ACGT	3 cut(s)	1647, 1926, 3087
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1164, 1214, 1808, 2032, 2212, 2264, 3268
HpySE526I	ACGT	3 cut(s)	1647, 1926, 3087
HspAI	GCGC	1 cut(s)	365
Kpn2I	TCCGGA	1 cut(s)	3465
Ksp22I	TGATCA	3 cut(s)	348, 2677, 3384
KspAI	GTTAAC	1 cut(s)	3106
LguI	GCTCTTC	1 cut(s)	3048
LmnI	GCTCC	4 cut(s)	1073, 1604, 2134, 3319
LweI	GCATC	7 cut(s)	159, 798, 1195, 1885, 2215, 2504, 3331
MaeI	CTAG	9 cut(s)	194, 870, 1353, 1548, 1758, 2880, 2900, 3188, 3272
MaeII	ACGT	3 cut(s)	1647, 1926, 3087
MaeIII	GTNAC	8 cut(s)	16, 307, 1265, 1621, 1711, 1942, 3088, 3359
MbiI	CCGCTC	2 cut(s)	542, 1097
MfeI	CAATTG	1 cut(s)	1196
MflI	RGATCY	3 cut(s)	1528, 2425, 2875
MhlI	GDGCHC	3 cut(s)	268, 2067, 2530
MlyI	GAGTC	2 cut(s)	1400, 2500
MmeI	TCCRAC	2 cut(s)	141, 1431
MroI	TCCGGA	1 cut(s)	3465
MroXI	GAANNNNTTC	1 cut(s)	717
MslI	CAYNNNNRTG	1 cut(s)	2442
MspR9I	CCNGG	9 cut(s)	63, 94, 173, 212, 263, 313, 1142, 2812, 3144
MunI	CAATTG	1 cut(s)	1196
MvaI	CCWGG	3 cut(s)	212, 1142, 2812
MvnI	CGCG	2 cut(s)	44, 49
MwoI	GCNNNNNNNGC	7 cut(s)	1164, 1214, 1808, 2032, 2212, 2264, 3268
NciI	CCSGG	6 cut(s)	63, 94, 173, 263, 313, 3144
NdeI	CATATG	2 cut(s)	1444, 2164
NheI	GCTAGC	1 cut(s)	3271
NlaIV	GGNNCC	2 cut(s)	60, 2877
NmeAIII	GCCGAG	1 cut(s)	126
NmuCI	GTSAC	6 cut(s)	16, 307, 1265, 1621, 1942, 3359
NsbI	TGCGCA	1 cut(s)	366
NspI	RCATGY	3 cut(s)	1772, 2810, 3442
NspV	TTCGAA	1 cut(s)	1702
PaeI	GCATGC	1 cut(s)	2810
PagI	TCATGA	1 cut(s)	2029
PciSI	GCTCTTC	1 cut(s)	3048
PdmI	GAANNNNTTC	1 cut(s)	717
PfeI	GAWTC	9 cut(s)	66, 128, 385, 481, 2107, 2603, 2621, 2651, 2672
Pfl23II	CGTACG	1 cut(s)	39
PinAI	ACCGGT	1 cut(s)	1437
PleI	GAGTC	2 cut(s)	1399, 2500
PpsI	GAGTC	2 cut(s)	1399, 2500
PshAI	GACNNNNGTC	1 cut(s)	2349
PshBI	ATTAAT	1 cut(s)	2661
Psp1406I	AACGTT	2 cut(s)	1647, 3087
Psp6I	CCWGG	3 cut(s)	210, 1140, 2810
PspEI	GGTNACC	1 cut(s)	16
PspFI	CCCAGC	2 cut(s)	506, 2613
PspGI	CCWGG	3 cut(s)	210, 1140, 2810
PspLI	CGTACG	1 cut(s)	39
PspN4I	GGNNCC	2 cut(s)	60, 2877
PspPI	GGNCC	6 cut(s)	259, 289, 379, 1411, 1435, 3504
PstI	CTGCAG	3 cut(s)	748, 2269, 2913
PsuI	RGATCY	3 cut(s)	1528, 2425, 2875
RsaI	GTAC	8 cut(s)	5, 41, 200, 284, 344, 1139, 1669, 2987
RsaNI	GTAC	8 cut(s)	4, 40, 199, 283, 343, 1138, 1668, 2986
RseI	CAYNNNNRTG	1 cut(s)	2442
Rsr2I	CGGWCCG	1 cut(s)	259
RsrII	CGGWCCG	1 cut(s)	259
SapI	GCTCTTC	1 cut(s)	3048
Sau96I	GGNCC	6 cut(s)	259, 289, 379, 1411, 1435, 3504
ScaI	AGTACT	1 cut(s)	344
SchI	GAGTC	2 cut(s)	1400, 2500
ScrFI	CCNGG	9 cut(s)	63, 94, 173, 212, 263, 313, 1142, 2812, 3144
SduI	GDGCHC	3 cut(s)	268, 2067, 2530
SfaNI	GCATC	7 cut(s)	159, 798, 1195, 1885, 2215, 2504, 3331
SfcI	CTRYAG	5 cut(s)	744, 1218, 1332, 2265, 2909
SfuI	TTCGAA	1 cut(s)	1702
SinI	GGWCC	4 cut(s)	259, 379, 1435, 3504
SmiMI	CAYNNNNRTG	1 cut(s)	2442
SmlI	CTYRAG	6 cut(s)	554, 921, 2321, 2429, 2930, 3197
SmoI	CTYRAG	6 cut(s)	554, 921, 2321, 2429, 2930, 3197
SphI	GCATGC	1 cut(s)	2810
SsiI	CCGC	9 cut(s)	49, 176, 422, 473, 540, 1097, 1167, 1996, 2872
SspI	AATATT	1 cut(s)	3373
SspMI	CTAG	9 cut(s)	194, 870, 1353, 1548, 1758, 2880, 2900, 3188, 3272
StyD4I	CCNGG	9 cut(s)	61, 92, 171, 210, 261, 311, 1140, 2810, 3142
StyI	CCWWGG	3 cut(s)	453, 618, 1757
TaiI	ACGT	3 cut(s)	1650, 1929, 3090
TaqI	TCGA	5 cut(s)	69, 126, 131, 249, 1702
TatI	WGTACW	4 cut(s)	3, 198, 342, 2985
TauI	GCSGC	3 cut(s)	179, 542, 1998
TfiI	GAWTC	9 cut(s)	66, 128, 385, 481, 2107, 2603, 2621, 2651, 2672
TscAI	CASTG	6 cut(s)	748, 1274, 1392, 1630, 1978, 3432
TseFI	GTSAC	6 cut(s)	16, 307, 1265, 1621, 1942, 3359
Tsp45I	GTSAC	6 cut(s)	16, 307, 1265, 1621, 1942, 3359
TspGWI	ACGGA	1 cut(s)	2737
TspRI	CASTG	6 cut(s)	748, 1274, 1392, 1630, 1978, 3432
VpaK11BI	GGWCC	4 cut(s)	259, 379, 1435, 3504
VspI	ATTAAT	1 cut(s)	2661
XagI	CCTNNNNNAGG	1 cut(s)	3171
XapI	RAATTY	7 cut(s)	777, 1260, 1294, 1734, 2260, 2578, 3473
XceI	RCATGY	3 cut(s)	1772, 2810, 3442
XmaJI	CCTAGG	1 cut(s)	1757
XmiI	GTMKAC	2 cut(s)	1656, 2787
XmnI	GAANNNNTTC	1 cut(s)	717
XspI	CTAG	9 cut(s)	194, 870, 1353, 1548, 1758, 2880, 2900, 3188, 3272
ZrmI	AGTACT	1 cut(s)	344

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.