FvH4_7g28800

Structural maintenance of chromosomes protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0003832
Physical Location & Seq
Forward (+)
21292216 .. 21293774
1559 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g28800.t1

Sequence Viewer

Length: 717 bp
ATGTACATCAAGGAGGTAACCTTGGAAGGAATCAAGTCCAGAAAATCTTACAATCAAACGCGGACGGTGGTGCCGGGTTTCGACCCGTTTTTCAAAGCCATAACGGGGCTAAACGGGTCGGGTAAGTCCAACATCCTCGATTCGATTTGCTTCGTTCTGGGCATCACCAATTTACAGCAAGTCAGGGCCGCCAATCTGCAGGAGCTGGTGTATAAGCAAGGCCAGGCCGGAAGGCCACCATGTCAATCGTCTTCCACAATTCGGACCGGGCTCGCAGTCCTCTCGGAAGCTGCCGGGACGAGAATGTATGAGACTAAAAAGGAAGCGGCTTTAAAAACTCTTGAGTTGATGAGATTAATAAGCTTCTTGACCATGAGATATTGCCTGCCTTGGACAAGTTCTGGAGACAAAGGACACAGTATATGCAATGGGCTAAATGGTAATGCTGACTTGGATCGGCTTAAAAGGTTTTGCATTGCTTATGAATATGTTCAAGCGGAGAGGATTAGAGACAGTGCAGTTTGTGAGGTGGAACAAGTGAAGGCCAAAATTTCTGAGGTTGACGATGATAAAGGAAATGGAGATACAAGTGTCCAAGATGAGATTGAAGATATTGGAAGCAGTGATGAGAAGAAAGGGGAGGCTGTTGGGAGCATGAGCAGAGTCGATTTGCAGAAGAGGCTTAGTGCTGCAGAGGAAGAGGAGGATTTGAGCTGA

Protein Analysis

239

Amino Acids

26.21

Weight (kDa)

5.31

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_N PF02463 3 - 76 2.2e-17 RecF/RecN/SMC N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 70
AccII CGCG 1 cut(s) 61
AciI CCGC 4 cut(s) 61, 189, 326, 497
AclWI GGATC 1 cut(s) 462
AcsI RAATTY 1 cut(s) 549
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 105, 261
AgsI TTSAA 3 cut(s) 94, 494, 608
AjnI CCWGG 1 cut(s) 222
AluBI AGCT 4 cut(s) 205, 290, 363, 714
AluI AGCT 4 cut(s) 205, 290, 363, 714
Alw26I GTCTC 3 cut(s) 305, 399, 504
AlwI GGATC 1 cut(s) 462
AlwNI CAGNNNCTG 1 cut(s) 205
AoxI GGCC 5 cut(s) 186, 220, 225, 233, 543
ApeKI GCWGC 2 cut(s) 290, 689
ApoI RAATTY 1 cut(s) 549
AseI ATTAAT 1 cut(s) 356
Asp700I GAANNNNTTC 1 cut(s) 489
AspS9I GGNCC 2 cut(s) 186, 264
AsuC2I CCSGG 3 cut(s) 75, 268, 295
AsuHPI GGTGA 1 cut(s) 157
AvaII GGWCC 1 cut(s) 264
BaeI ACNNNNGTAYC 2 cut(s) 576, 609
BanI GGYRCC 1 cut(s) 70
BanII GRGCYC 1 cut(s) 273
BbsI GAAGAC 1 cut(s) 243
BbvI GCAGC 2 cut(s) 277, 676
BcgI CGANNNNNNTGC 2 cut(s) 264, 298
BciT130I CCWGG 1 cut(s) 224
BcnI CCSGG 3 cut(s) 75, 268, 295
BcoDI GTCTC 3 cut(s) 305, 399, 504
BfmI CTRYAG 2 cut(s) 197, 690
BisI GCNGC 4 cut(s) 189, 291, 327, 690
BlsI GCNGC 4 cut(s) 190, 292, 328, 691
Bme1390I CCNGG 4 cut(s) 75, 224, 268, 295
Bme18I GGWCC 1 cut(s) 264
BmgT120I GGNCC 2 cut(s) 186, 264
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 4 cut(s) 75, 224, 268, 295
BmsI GCATC 1 cut(s) 171
BpiI GAAGAC 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 362
BpuMI CCSGG 3 cut(s) 75, 268, 295
BsaJI CCNNGG 2 cut(s) 21, 389
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 261
Bse3DI GCAATG 2 cut(s) 433, 474
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 2 cut(s) 21, 389
BseGI GGATG 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 105, 261
BseMI GCAATG 2 cut(s) 433, 474
BseMII CTCAG 1 cut(s) 546
BseRI GAGGAG 1 cut(s) 716
BseXI GCAGC 2 cut(s) 277, 676
BsgI GTGCAG 1 cut(s) 537
Bsh1236I CGCG 1 cut(s) 61
BshFI GGCC 5 cut(s) 188, 222, 227, 235, 545
BshNI GGYRCC 1 cut(s) 70
BsiSI CCGG 4 cut(s) 74, 228, 267, 294
BslFI GGGAC 1 cut(s) 310
BslI CCNNNNNNNGG 2 cut(s) 105, 261
BsmAI GTCTC 3 cut(s) 305, 399, 504
BsmFI GGGAC 1 cut(s) 310
BsnI GGCC 5 cut(s) 188, 222, 227, 235, 545
Bsp1286I GDGCHC 1 cut(s) 273
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 454
BspACI CCGC 4 cut(s) 61, 189, 326, 497
BspANI GGCC 5 cut(s) 188, 222, 227, 235, 545
BspCNI CTCAG 1 cut(s) 547
BspFNI CGCG 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 72
BspMAI CTGCAG 2 cut(s) 201, 694
BspPI GGATC 1 cut(s) 462
BspT107I GGYRCC 1 cut(s) 70
BsrDI GCAATG 2 cut(s) 433, 474
BsrGI TGTACA 1 cut(s) 3
BssECI CCNNGG 2 cut(s) 21, 389
BssMI GATC 1 cut(s) 454
BssT1I CCWWGG 2 cut(s) 21, 389
Bst2UI CCWGG 1 cut(s) 224
Bst4CI ACNGT 3 cut(s) 67, 419, 515
Bst6I CTCTTC 2 cut(s) 671, 693
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 273, 386
BstDEI CTNAG 2 cut(s) 555, 683
BstEII GGTNACC 1 cut(s) 16
BstF5I GGATG 1 cut(s) 132
BstFNI CGCG 1 cut(s) 61
BstKTI GATC 1 cut(s) 457
BstMAI GTCTC 3 cut(s) 305, 399, 504
BstMBI GATC 1 cut(s) 454
BstMWI GCNNNNNNNGC 1 cut(s) 679
BstNI CCWGG 1 cut(s) 224
BstPI GGTNACC 1 cut(s) 16
BstSCI CCNGG 4 cut(s) 73, 222, 266, 293
BstSFI CTRYAG 2 cut(s) 197, 690
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 2 cut(s) 277, 676
BstV2I GAAGAC 1 cut(s) 243
BsuRI GGCC 5 cut(s) 188, 222, 227, 235, 545
BtsCI GGATG 1 cut(s) 132
BtsI GCAGTG 1 cut(s) 628
BtsIMutI CAGTG 2 cut(s) 520, 628
Cac8I GCNNGC 2 cut(s) 273, 386
CaiI CAGNNNCTG 1 cut(s) 205
Cfr13I GGNCC 2 cut(s) 186, 264
CpoI CGGWCCG 1 cut(s) 264
Csp6I GTAC 1 cut(s) 4
CspI CGGWCCG 1 cut(s) 264
CviAII CATG 3 cut(s) 240, 373, 655
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 2 cut(s) 555, 683
DpnI GATC 1 cut(s) 456
DpnII GATC 1 cut(s) 454
DraI TTTAAA 1 cut(s) 333
Eam1104I CTCTTC 2 cut(s) 671, 693
EarI CTCTTC 2 cut(s) 671, 693
Eco130I CCWWGG 2 cut(s) 21, 389
Eco24I GRGCYC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 264
Eco91I GGTNACC 1 cut(s) 16
EcoO65I GGTNACC 1 cut(s) 16
EcoRII CCWGG 1 cut(s) 222
EcoT14I CCWWGG 2 cut(s) 21, 389
EcoT38I GRGCYC 1 cut(s) 273
ErhI CCWWGG 2 cut(s) 21, 389
FaeI CATG 3 cut(s) 243, 376, 658
FaqI GGGAC 1 cut(s) 310
FatI CATG 3 cut(s) 239, 372, 654
Fnu4HI GCNGC 4 cut(s) 189, 291, 327, 690
FokI GGATG 1 cut(s) 119
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 4 cut(s) 189, 291, 327, 690
GluI GCNGC 4 cut(s) 189, 291, 327, 690
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 5 cut(s) 188, 222, 227, 235, 545
HapII CCGG 4 cut(s) 74, 228, 267, 294
Hin1II CATG 3 cut(s) 243, 376, 658
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 1 cut(s) 361
HinfI GANTC 3 cut(s) 30, 140, 663
HpaII CCGG 4 cut(s) 74, 228, 267, 294
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 3 cut(s) 264, 286, 556
Hpy188III TCNNGA 4 cut(s) 39, 341, 367, 402
Hpy8I GTNNAC 1 cut(s) 562
HpyAV CCTTC 3 cut(s) 20, 225, 535
HpyCH4III ACNGT 3 cut(s) 67, 419, 515
HpyCH4V TGCA 6 cut(s) 199, 426, 474, 518, 673, 692
HpyF10VI GCNNNNNNNGC 1 cut(s) 679
HpyF3I CTNAG 2 cut(s) 555, 683
Hsp92II CATG 3 cut(s) 243, 376, 658
Kzo9I GATC 1 cut(s) 454
LmnI GCTCC 2 cut(s) 202, 651
Lsp1109I GCAGC 2 cut(s) 277, 676
LweI GCATC 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 16
MalI GATC 1 cut(s) 456
MboI GATC 1 cut(s) 454
MboII GAAGA 5 cut(s) 243, 620, 643, 688, 710
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 3 cut(s) 169, 258, 549
MlyI GAGTC 1 cut(s) 672
MmeI TCCRAC 1 cut(s) 153
MroXI GAANNNNTTC 1 cut(s) 489
MseI TTAA 3 cut(s) 332, 356, 462
MspI CCGG 4 cut(s) 74, 228, 267, 294
MspR9I CCNGG 4 cut(s) 75, 224, 268, 295
MvaI CCWGG 1 cut(s) 224
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 1 cut(s) 679
NciI CCSGG 3 cut(s) 75, 268, 295
NdeII GATC 1 cut(s) 454
NlaIII CATG 3 cut(s) 243, 376, 658
NlaIV GGNNCC 1 cut(s) 72
PdmI GAANNNNTTC 1 cut(s) 489
PfeI GAWTC 2 cut(s) 30, 140
PkrI GCNGC 4 cut(s) 190, 292, 328, 691
PleI GAGTC 1 cut(s) 671
PpsI GAGTC 1 cut(s) 671
PshBI ATTAAT 1 cut(s) 356
Psp6I CCWGG 1 cut(s) 222
PspEI GGTNACC 1 cut(s) 16
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 2 cut(s) 186, 264
PstI CTGCAG 2 cut(s) 201, 694
PstNI CAGNNNCTG 1 cut(s) 205
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
Rsr2I CGGWCCG 1 cut(s) 264
RsrII CGGWCCG 1 cut(s) 264
SaqAI TTAA 3 cut(s) 332, 356, 462
SatI GCNGC 4 cut(s) 189, 291, 327, 690
Sau3AI GATC 1 cut(s) 454
Sau96I GGNCC 2 cut(s) 186, 264
SchI GAGTC 1 cut(s) 672
ScrFI CCNGG 4 cut(s) 75, 224, 268, 295
SduI GDGCHC 1 cut(s) 273
SetI ASST 9 cut(s) 18, 23, 207, 292, 365, 470, 531, 561, 716
SfaNI GCATC 1 cut(s) 171
SfcI CTRYAG 2 cut(s) 197, 690
SinI GGWCC 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 3 cut(s) 169, 258, 549
SsiI CCGC 4 cut(s) 61, 189, 326, 497
StyD4I CCNGG 4 cut(s) 73, 222, 266, 293
StyI CCWWGG 2 cut(s) 21, 389
TaaI ACNGT 3 cut(s) 67, 419, 515
TaqI TCGA 4 cut(s) 81, 138, 143, 666
TasI AATT 3 cut(s) 169, 258, 549
TatI WGTACW 1 cut(s) 3
TauI GCSGC 2 cut(s) 191, 329
TfiI GAWTC 2 cut(s) 30, 140
Tru1I TTAA 3 cut(s) 332, 356, 462
Tru9I TTAA 3 cut(s) 332, 356, 462
TscAI CASTG 2 cut(s) 520, 628
TseI GCWGC 2 cut(s) 290, 689
TspDTI ATGAA 1 cut(s) 498
TspRI CASTG 2 cut(s) 520, 628
VpaK11BI GGWCC 1 cut(s) 264
VspI ATTAAT 1 cut(s) 356
XapI RAATTY 1 cut(s) 549
XmnI GAANNNNTTC 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.