FvH4_7g31360

At4g00950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009927
Physical Location & Seq
Reverse (-)
22681919 .. 22684326
2408 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31360.t1

Sequence Viewer

Length: 678 bp
ATGGAGGAGTCTGAGGCTGCAGTGAATGAAGCAAGTCCCATAAGGAAGCTGGCATTATTCTCGCTTCCAAGCCAGCCACCCGAGCCTCCAGGGATACTGACGCCGCCGCTGCTGACGTCAGTTTCAGTTCCGTTCCAGTGGGAGGAGGCGCCGGGAAAGCCCCGACACTCCGGAGAAGGCGCAGAGCCCAAGGCCAAGTGCGCGAGATCATTGGAGCTCCCTCCGAGGATGTTGGTGGGTAGTAGTGACGCTAAAGTTACCAACATGCCCTCCCCAACGACTGTGCTGGATGGGCCTGAGGTGGGCCGGACTTTGTCCTTTTCGTATGCATTGAGGAGCCCGAGCAAGGAGATTAAGGGCGGCCGTGGCGGCGGCGGTAGCGGCAGGTTTGGGTCGAAGAGGTGGGCCGGTTTCAGGAAGAACAAGGAGGCCGGTGAGGGTGGTTTCGACTTTTTGTCTACCGATAATGGCGGTGATCAGACGAAGGTGAAGATCACAAGGGTTAAGAGGAAGGCTAGCTTCTTCAATGGTGCTCATCACTCAAGGTCACATATGTGGGCCAGCATATATGAAAGCTTAAAGCAGGTGGTTCCATGGAGACGCAGACAAGAAAAACTGAGAAAATCTGCAACTGTTGGATATGAACCTCAAAACTGTGCTCCAATTCCCACTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF688

Protein Analysis

226

Amino Acids

24.44

Weight (kDa)

9.85

Isoelectric Point (pI)

58.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 574
AatII GACGTC 1 cut(s) 119
Acc36I ACCTGC 2 cut(s) 375, 574
AccB1I GGYRCC 1 cut(s) 148
AccI GTMKAC 1 cut(s) 458
AccII CGCG 1 cut(s) 203
AccIII TCCGGA 1 cut(s) 170
AciI CCGC 8 cut(s) 104, 107, 360, 369, 372, 375, 381, 471
AcoI YGGCCR 1 cut(s) 361
AcyI GRCGYC 3 cut(s) 101, 116, 149
AfiI CCNNNNNNNGG 4 cut(s) 142, 302, 346, 414
AgsI TTSAA 1 cut(s) 526
AhdI GACNNNNNGTC 1 cut(s) 454
AjnI CCWGG 1 cut(s) 88
AleI CACNNNNGTG 1 cut(s) 553
AluBI AGCT 4 cut(s) 49, 217, 519, 576
AluI AGCT 4 cut(s) 49, 217, 519, 576
Alw21I GWGCWC 3 cut(s) 219, 535, 661
Alw26I GTCTC 1 cut(s) 592
Ama87I CYCGRG 2 cut(s) 80, 340
Aor13HI TCCGGA 1 cut(s) 170
AoxI GGCC 7 cut(s) 192, 293, 304, 361, 405, 429, 558
ApeKI GCWGC 2 cut(s) 17, 109
AspLEI GCGC 3 cut(s) 151, 182, 203
AspS9I GGNCC 4 cut(s) 293, 304, 405, 558
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 3 cut(s) 446, 485, 499
AsuNHI GCTAGC 1 cut(s) 515
AvaI CYCGRG 2 cut(s) 80, 340
AxyI CCTNAGG 1 cut(s) 297
BanI GGYRCC 1 cut(s) 148
BanII GRGCYC 3 cut(s) 189, 219, 341
Bbv12I GWGCWC 3 cut(s) 219, 535, 661
BbvI GCAGC 2 cut(s) 4, 96
BccI CCATC 1 cut(s) 284
BceAI ACGGC 1 cut(s) 348
BcgI CGANNNNNNTGC 2 cut(s) 42, 76
BciT130I CCWGG 1 cut(s) 90
BciVI GTATCC 1 cut(s) 87
BclI TGATCA 1 cut(s) 475
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 1 cut(s) 592
BfaI CTAG 1 cut(s) 516
BfmI CTRYAG 1 cut(s) 18
BfoI RGCGCY 1 cut(s) 152
BfuAI ACCTGC 2 cut(s) 375, 574
BfuI GTATCC 1 cut(s) 87
BglI GCCNNNNNGGC 1 cut(s) 369
BisI GCNGC 8 cut(s) 18, 104, 107, 110, 361, 370, 373, 382
BlsI GCNGC 8 cut(s) 19, 105, 108, 111, 362, 371, 374, 383
Bme1390I CCNGG 2 cut(s) 90, 153
BmeRI GACNNNNNGTC 1 cut(s) 454
BmeT110I CYCGRG 2 cut(s) 80, 340
BmgT120I GGNCC 4 cut(s) 293, 304, 405, 558
BmiI GGNNCC 3 cut(s) 150, 338, 591
BmrFI CCNGG 2 cut(s) 90, 153
BmtI GCTAGC 1 cut(s) 519
BpmI CTGGAG 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 526
BpuMI CCSGG 1 cut(s) 153
BsaHI GRCGYC 3 cut(s) 101, 116, 149
BsaJI CCNNGG 5 cut(s) 89, 189, 224, 364, 593
BsaWI WCCGGW 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 302, 346, 414
Bse118I RCCGGY 2 cut(s) 407, 431
Bse1I ACTGG 1 cut(s) 136
Bse21I CCTNAGG 1 cut(s) 297
BseAI TCCGGA 1 cut(s) 170
BseBI CCWGG 1 cut(s) 90
BseDI CCNNGG 5 cut(s) 89, 189, 224, 364, 593
BseGI GGATG 2 cut(s) 234, 295
BseLI CCNNNNNNNGG 4 cut(s) 142, 302, 346, 414
BseMII CTCAG 2 cut(s) 288, 608
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 3 cut(s) 20, 158, 349
BseX3I CGGCCG 1 cut(s) 361
BseXI GCAGC 2 cut(s) 4, 96
Bsh1236I CGCG 1 cut(s) 203
Bsh1285I CGRYCG 1 cut(s) 364
BshFI GGCC 7 cut(s) 194, 295, 306, 363, 407, 431, 560
BshNI GGYRCC 1 cut(s) 148
BsiEI CGRYCG 1 cut(s) 364
BsiHKAI GWGCWC 3 cut(s) 219, 535, 661
BsiHKCI CYCGRG 2 cut(s) 80, 340
BsiSI CCGG 5 cut(s) 152, 171, 307, 408, 432
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 4 cut(s) 142, 302, 346, 414
BsmAI GTCTC 1 cut(s) 592
BsmBI CGTCTC 1 cut(s) 592
BsmFI GGGAC 1 cut(s) 21
BsnI GGCC 7 cut(s) 194, 295, 306, 363, 407, 431, 560
BsoBI CYCGRG 2 cut(s) 80, 340
Bsp1286I GDGCHC 5 cut(s) 189, 219, 341, 535, 661
Bsp13I TCCGGA 1 cut(s) 170
Bsp143I GATC 3 cut(s) 206, 475, 492
Bsp19I CCATGG 1 cut(s) 593
BspACI CCGC 8 cut(s) 104, 107, 360, 369, 372, 375, 381, 471
BspANI GGCC 7 cut(s) 194, 295, 306, 363, 407, 431, 560
BspCNI CTCAG 3 cut(s) 4, 289, 609
BspEI TCCGGA 1 cut(s) 170
BspFNI CGCG 1 cut(s) 203
BspLI GGNNCC 3 cut(s) 150, 338, 591
BspMAI CTGCAG 1 cut(s) 22
BspMI ACCTGC 2 cut(s) 375, 574
BspOI GCTAGC 1 cut(s) 519
BspT107I GGYRCC 1 cut(s) 148
BsrFI RCCGGY 2 cut(s) 407, 431
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 2 cut(s) 407, 431
BssECI CCNNGG 5 cut(s) 89, 189, 224, 364, 593
BssMI GATC 3 cut(s) 206, 475, 492
BssNI GRCGYC 3 cut(s) 101, 116, 149
BssT1I CCWWGG 2 cut(s) 189, 593
Bst2UI CCWGG 1 cut(s) 90
Bst4CI ACNGT 3 cut(s) 283, 634, 656
Bst6I CTCTTC 1 cut(s) 392
BstACI GRCGYC 3 cut(s) 101, 116, 149
BstC8I GCNNGC 4 cut(s) 51, 74, 517, 562
BstDEI CTNAG 3 cut(s) 12, 297, 617
BstDSI CCRYGG 2 cut(s) 364, 593
BstF5I GGATG 2 cut(s) 234, 295
BstFNI CGCG 1 cut(s) 203
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 3 cut(s) 151, 182, 203
BstKTI GATC 3 cut(s) 209, 478, 495
BstMAI GTCTC 1 cut(s) 592
BstMBI GATC 3 cut(s) 206, 475, 492
BstMCI CGRYCG 1 cut(s) 364
BstMWI GCNNNNNNNGC 9 cut(s) 82, 109, 157, 200, 292, 366, 369, 378, 381
BstNI CCWGG 1 cut(s) 90
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 2 cut(s) 88, 151
BstSFI CTRYAG 1 cut(s) 18
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 2 cut(s) 4, 96
BstZI CGGCCG 1 cut(s) 361
Bsu36I CCTNAGG 1 cut(s) 297
BsuI GTATCC 1 cut(s) 87
BsuRI GGCC 7 cut(s) 194, 295, 306, 363, 407, 431, 560
BtgI CCRYGG 2 cut(s) 364, 593
BtsCI GGATG 2 cut(s) 234, 295
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 2 cut(s) 27, 143
BveI ACCTGC 2 cut(s) 375, 574
Cac8I GCNNGC 4 cut(s) 51, 74, 517, 562
CfoI GCGC 3 cut(s) 151, 182, 203
Cfr10I RCCGGY 2 cut(s) 407, 431
Cfr13I GGNCC 4 cut(s) 293, 304, 405, 558
CseI GACGC 3 cut(s) 109, 257, 609
CviAII CATG 2 cut(s) 265, 594
DdeI CTNAG 3 cut(s) 12, 297, 617
DinI GGCGCC 1 cut(s) 150
DpnI GATC 3 cut(s) 208, 477, 494
DpnII GATC 3 cut(s) 206, 475, 492
DriI GACNNNNNGTC 1 cut(s) 454
EaeI YGGCCR 1 cut(s) 361
EagI CGGCCG 1 cut(s) 361
Eam1104I CTCTTC 1 cut(s) 392
Eam1105I GACNNNNNGTC 1 cut(s) 454
EarI CTCTTC 1 cut(s) 392
Ecl136II GAGCTC 1 cut(s) 217
EclXI CGGCCG 1 cut(s) 361
Eco130I CCWWGG 2 cut(s) 189, 593
Eco24I GRGCYC 3 cut(s) 189, 219, 341
Eco52I CGGCCG 1 cut(s) 361
Eco53kI GAGCTC 1 cut(s) 217
Eco81I CCTNAGG 1 cut(s) 297
Eco88I CYCGRG 2 cut(s) 80, 340
EcoICRI GAGCTC 1 cut(s) 217
EcoRII CCWGG 1 cut(s) 88
EcoT14I CCWWGG 2 cut(s) 189, 593
EcoT22I ATGCAT 1 cut(s) 331
EcoT38I GRGCYC 3 cut(s) 189, 219, 341
EgeI GGCGCC 1 cut(s) 150
EheI GGCGCC 1 cut(s) 150
ErhI CCWWGG 2 cut(s) 189, 593
Esp3I CGTCTC 1 cut(s) 592
FaeI CATG 2 cut(s) 268, 597
FalI AAGNNNNNCTT 2 cut(s) 503, 535
FaqI GGGAC 1 cut(s) 21
FatI CATG 2 cut(s) 264, 593
FauNDI CATATG 1 cut(s) 552
FbaI TGATCA 1 cut(s) 475
FblI GTMKAC 1 cut(s) 458
Fnu4HI GCNGC 8 cut(s) 18, 104, 107, 110, 361, 370, 373, 382
FokI GGATG 2 cut(s) 241, 302
FriOI GRGCYC 3 cut(s) 189, 219, 341
Fsp4HI GCNGC 8 cut(s) 18, 104, 107, 110, 361, 370, 373, 382
FspBI CTAG 1 cut(s) 516
GlaI GCGC 3 cut(s) 150, 181, 202
GluI GCNGC 8 cut(s) 18, 104, 107, 110, 361, 370, 373, 382
GsuI CTGGAG 1 cut(s) 72
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 7 cut(s) 194, 295, 306, 363, 407, 431, 560
HapII CCGG 5 cut(s) 152, 171, 307, 408, 432
HgaI GACGC 3 cut(s) 109, 257, 609
HhaI GCGC 3 cut(s) 151, 182, 203
Hin1I GRCGYC 3 cut(s) 101, 116, 149
Hin1II CATG 2 cut(s) 268, 597
Hin6I GCGC 3 cut(s) 149, 180, 201
HinP1I GCGC 3 cut(s) 149, 180, 201
HindIII AAGCTT 1 cut(s) 574
HinfI GANTC 1 cut(s) 8
HpaII CCGG 5 cut(s) 152, 171, 307, 408, 432
HphI GGTGA 3 cut(s) 446, 485, 499
Hpy166II GTNNAC 1 cut(s) 459
Hpy188I TCNGA 3 cut(s) 13, 225, 480
Hpy188III TCNNGA 2 cut(s) 171, 415
Hpy8I GTNNAC 1 cut(s) 459
HpyAV CCTTC 3 cut(s) 170, 478, 505
HpyCH4III ACNGT 3 cut(s) 283, 634, 656
HpyCH4IV ACGT 1 cut(s) 116
HpyCH4V TGCA 3 cut(s) 20, 329, 629
HpyF10VI GCNNNNNNNGC 9 cut(s) 82, 109, 157, 200, 292, 366, 369, 378, 381
HpyF3I CTNAG 3 cut(s) 12, 297, 617
HpySE526I ACGT 1 cut(s) 116
Hsp92I GRCGYC 3 cut(s) 101, 116, 149
Hsp92II CATG 2 cut(s) 268, 597
HspAI GCGC 3 cut(s) 149, 180, 201
KasI GGCGCC 1 cut(s) 148
Kpn2I TCCGGA 1 cut(s) 170
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 3 cut(s) 206, 475, 492
LmnI GCTCC 4 cut(s) 214, 222, 336, 664
Lsp1109I GCAGC 2 cut(s) 4, 96
MaeI CTAG 1 cut(s) 516
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 3 cut(s) 245, 256, 546
MalI GATC 3 cut(s) 208, 477, 494
MboI GATC 3 cut(s) 206, 475, 492
MboII GAAGA 4 cut(s) 409, 430, 502, 514
MhlI GDGCHC 5 cut(s) 189, 219, 341, 535, 661
MluCI AATT 1 cut(s) 663
Mly113I GGCGCC 1 cut(s) 149
MlyI GAGTC 1 cut(s) 17
MmeI TCCRAC 1 cut(s) 616
Mph1103I ATGCAT 1 cut(s) 331
MroI TCCGGA 1 cut(s) 170
MseI TTAA 3 cut(s) 354, 504, 578
MslI CAYNNNNRTG 1 cut(s) 553
MspA1I CMGCKG 1 cut(s) 109
MspI CCGG 5 cut(s) 152, 171, 307, 408, 432
MspR9I CCNGG 2 cut(s) 90, 153
MvaI CCWGG 1 cut(s) 90
MvnI CGCG 1 cut(s) 203
MwoI GCNNNNNNNGC 9 cut(s) 82, 109, 157, 200, 292, 366, 369, 378, 381
NarI GGCGCC 1 cut(s) 149
NciI CCSGG 1 cut(s) 153
NcoI CCATGG 1 cut(s) 593
NdeI CATATG 1 cut(s) 552
NdeII GATC 3 cut(s) 206, 475, 492
NheI GCTAGC 1 cut(s) 515
NlaIII CATG 2 cut(s) 268, 597
NlaIV GGNNCC 3 cut(s) 150, 338, 591
NmuCI GTSAC 2 cut(s) 245, 546
NsiI ATGCAT 1 cut(s) 331
NspI RCATGY 1 cut(s) 268
OliI CACNNNNGTG 1 cut(s) 553
PaqCI CACCTGC 1 cut(s) 574
PflFI GACNNNGTC 1 cut(s) 313
PkrI GCNGC 8 cut(s) 19, 105, 108, 111, 362, 371, 374, 383
PleI GAGTC 1 cut(s) 16
PluTI GGCGCC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 16
Psp124BI GAGCTC 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 88
PspGI CCWGG 1 cut(s) 88
PspN4I GGNNCC 3 cut(s) 150, 338, 591
PspPI GGNCC 4 cut(s) 293, 304, 405, 558
PstI CTGCAG 1 cut(s) 22
PsyI GACNNNGTC 1 cut(s) 313
RseI CAYNNNNRTG 1 cut(s) 553
SacI GAGCTC 1 cut(s) 219
SaqAI TTAA 3 cut(s) 354, 504, 578
SatI GCNGC 8 cut(s) 18, 104, 107, 110, 361, 370, 373, 382
Sau3AI GATC 3 cut(s) 206, 475, 492
Sau96I GGNCC 4 cut(s) 293, 304, 405, 558
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 2 cut(s) 90, 153
SduI GDGCHC 5 cut(s) 189, 219, 341, 535, 661
SfcI CTRYAG 1 cut(s) 18
SfoI GGCGCC 1 cut(s) 150
SmiMI CAYNNNNRTG 1 cut(s) 553
SmlI CTYRAG 1 cut(s) 541
SmoI CTYRAG 1 cut(s) 541
Sse9I AATT 1 cut(s) 663
SsiI CCGC 8 cut(s) 104, 107, 360, 369, 372, 375, 381, 471
SspDI GGCGCC 1 cut(s) 148
SspMI CTAG 1 cut(s) 516
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 2 cut(s) 88, 151
StyI CCWWGG 2 cut(s) 189, 593
TaaI ACNGT 3 cut(s) 283, 634, 656
TaiI ACGT 1 cut(s) 119
TaqI TCGA 2 cut(s) 395, 447
TasI AATT 1 cut(s) 663
TauI GCSGC 6 cut(s) 106, 109, 363, 372, 375, 384
Tru1I TTAA 3 cut(s) 354, 504, 578
Tru9I TTAA 3 cut(s) 354, 504, 578
TscAI CASTG 2 cut(s) 27, 143
TseFI GTSAC 2 cut(s) 245, 546
TseI GCWGC 2 cut(s) 17, 109
Tsp45I GTSAC 2 cut(s) 245, 546
TspDTI ATGAA 3 cut(s) 42, 585, 657
TspGWI ACGGA 1 cut(s) 120
TspRI CASTG 2 cut(s) 27, 143
Tth111I GACNNNGTC 1 cut(s) 313
XceI RCATGY 1 cut(s) 268
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 458
XspI CTAG 1 cut(s) 516
ZraI GACGTC 1 cut(s) 117
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.