Rroxscaffold_4G00279380

At4g00950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009927
Physical Location & Seq
Forward (+)
2185593 .. 2187413
1821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00279380.1

Sequence Viewer

Length: 672 bp
ATGGAGAAGGAGTCTGAGGCAGTGAACGAAGCAAGCCCCATAAGGAAGCTGGCTCTGTTCTCACTTCCAAGCCAGCCACCCGAGCCTCCTGGGATGCTGACGCCGCCGCTGCTGACGTCAGTTTCGGTTCCTTTCCAGTGGGAGGAGGCGCCGGGAAAGCCCCGACACTTCTGCGGCGGAGAAGGCGGCGGAGGAACAGAGACCAAGGCCAAGTGCGCGAGATCCTTGGAGCTGCCTCCGAGGTTGTTGGTGAGTGAGGCTAAAGTTATCAACATGCCTTCCCCGACGACTGTGCTGGATGGGCCTGAGGTGGGCCGGACTCTGTCGTTTTCGTATGCATTGAGGAGCCCGAGCAAGGAGGTTAAGGGCGGCGGCGGCGGCGGGGGGTTTGGGTCTATGAGGTGGGCCAGGTTTCTGAAGAACAAGGAGGCCGGTGAGGGCAATTTCGACTTTTCGCCTACTGACAGCGGCGGTGATCGGACAAAGGTGAAGATCACAAGGGTTAGGAGTAAGGCTAGCTTCTTCAATGGTGCTCATCACTCAAGGTCACACATGTGGGCCAGCATCTGTGAAAGCTTAAAGCAAGTGGTTCCATGGAGACGCAGACAGGAAAAACTGAGAAAATCTGCTACTGTTGGATATGAACCTCAAAATCCAATTCCCACTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF688

Protein Analysis

223

Amino Acids

24.2

Weight (kDa)

9.74

Isoelectric Point (pI)

60.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 148
AccII CGCG 1 cut(s) 218
AclWI GGATC 1 cut(s) 216
AcuI CTGAAG 1 cut(s) 437
AcyI GRCGYC 3 cut(s) 101, 116, 149
AfiI CCNNNNNNNGG 3 cut(s) 142, 311, 355
AflIII ACRYGT 1 cut(s) 552
AgsI TTSAA 1 cut(s) 526
AhlI ACTAGT 1 cut(s) 665
AjnI CCWGG 2 cut(s) 88, 407
AleI CACNNNNGTG 1 cut(s) 553
AluBI AGCT 4 cut(s) 49, 232, 519, 576
AluI AGCT 4 cut(s) 49, 232, 519, 576
Alw21I GWGCWC 1 cut(s) 535
Alw26I GTCTC 2 cut(s) 194, 592
AlwI GGATC 1 cut(s) 216
AlwNI CAGNNNCTG 1 cut(s) 567
Ama87I CYCGRG 2 cut(s) 80, 349
AoxI GGCC 6 cut(s) 207, 302, 313, 405, 429, 558
ApeKI GCWGC 2 cut(s) 109, 232
ArsI GACNNNNNNTTYG 2 cut(s) 106, 138
AspLEI GCGC 2 cut(s) 151, 218
AspS9I GGNCC 4 cut(s) 302, 313, 405, 558
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 4 cut(s) 262, 446, 485, 499
AsuNHI GCTAGC 1 cut(s) 515
AvaI CYCGRG 2 cut(s) 80, 349
AxyI CCTNAGG 1 cut(s) 306
BanI GGYRCC 1 cut(s) 148
BanII GRGCYC 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 535
BbvI GCAGC 2 cut(s) 96, 219
BccI CCATC 1 cut(s) 293
BcgI CGANNNNNNTGC 4 cut(s) 153, 187, 274, 308
BciT130I CCWGG 2 cut(s) 90, 409
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 2 cut(s) 194, 592
BcuI ACTAGT 1 cut(s) 665
BfaI CTAG 2 cut(s) 516, 666
BfoI RGCGCY 1 cut(s) 152
Bme1390I CCNGG 3 cut(s) 90, 153, 409
BmeT110I CYCGRG 2 cut(s) 80, 349
BmgT120I GGNCC 4 cut(s) 302, 313, 405, 558
BmiI GGNNCC 4 cut(s) 129, 150, 347, 591
BmrFI CCNGG 3 cut(s) 90, 153, 409
BmsI GCATC 2 cut(s) 84, 573
BmtI GCTAGC 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 526
BpuMI CCSGG 1 cut(s) 153
BsaHI GRCGYC 3 cut(s) 101, 116, 149
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 5 cut(s) 89, 204, 225, 239, 593
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 311, 355
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 1 cut(s) 136
Bse21I CCTNAGG 1 cut(s) 306
BseBI CCWGG 2 cut(s) 90, 409
BseDI CCNNGG 5 cut(s) 89, 204, 225, 239, 593
BseGI GGATG 2 cut(s) 99, 304
BseLI CCNNNNNNNGG 3 cut(s) 142, 311, 355
BseMII CTCAG 3 cut(s) 6, 297, 608
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 2 cut(s) 158, 358
BseXI GCAGC 2 cut(s) 96, 219
Bsh1236I CGCG 1 cut(s) 218
BshFI GGCC 6 cut(s) 209, 304, 315, 407, 431, 560
BshNI GGYRCC 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 535
BsiHKCI CYCGRG 2 cut(s) 80, 349
BsiSI CCGG 3 cut(s) 152, 316, 432
BslI CCNNNNNNNGG 3 cut(s) 142, 311, 355
BsmAI GTCTC 2 cut(s) 194, 592
BsmBI CGTCTC 1 cut(s) 592
BsnI GGCC 6 cut(s) 209, 304, 315, 407, 431, 560
Bso31I GGTCTC 1 cut(s) 194
BsoBI CYCGRG 2 cut(s) 80, 349
Bsp1286I GDGCHC 2 cut(s) 350, 535
Bsp143I GATC 3 cut(s) 221, 475, 492
Bsp19I CCATGG 1 cut(s) 593
BspANI GGCC 6 cut(s) 209, 304, 315, 407, 431, 560
BspCNI CTCAG 3 cut(s) 7, 298, 609
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 4 cut(s) 129, 150, 347, 591
BspOI GCTAGC 1 cut(s) 519
BspPI GGATC 1 cut(s) 216
BspT107I GGYRCC 1 cut(s) 148
BspTNI GGTCTC 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 5 cut(s) 89, 204, 225, 239, 593
BssMI GATC 3 cut(s) 221, 475, 492
BssNI GRCGYC 3 cut(s) 101, 116, 149
BssT1I CCWWGG 3 cut(s) 204, 225, 593
Bst2UI CCWGG 2 cut(s) 90, 409
Bst4CI ACNGT 2 cut(s) 292, 634
BstACI GRCGYC 3 cut(s) 101, 116, 149
BstC8I GCNNGC 5 cut(s) 34, 51, 74, 517, 562
BstDEI CTNAG 3 cut(s) 15, 306, 617
BstDSI CCRYGG 1 cut(s) 593
BstF5I GGATG 2 cut(s) 99, 304
BstFNI CGCG 1 cut(s) 218
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 2 cut(s) 151, 218
BstKTI GATC 3 cut(s) 224, 478, 495
BstMAI GTCTC 2 cut(s) 194, 592
BstMBI GATC 3 cut(s) 221, 475, 492
BstMWI GCNNNNNNNGC 9 cut(s) 82, 103, 109, 157, 183, 215, 301, 375, 378
BstNI CCWGG 2 cut(s) 90, 409
BstNSI RCATGY 2 cut(s) 277, 556
BstSCI CCNGG 3 cut(s) 88, 151, 407
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 2 cut(s) 96, 219
BstX2I RGATCY 1 cut(s) 221
BstYI RGATCY 1 cut(s) 221
Bsu36I CCTNAGG 1 cut(s) 306
BsuRI GGCC 6 cut(s) 209, 304, 315, 407, 431, 560
BtgI CCRYGG 1 cut(s) 593
BtsCI GGATG 2 cut(s) 99, 304
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 2 cut(s) 27, 143
Cac8I GCNNGC 5 cut(s) 34, 51, 74, 517, 562
CaiI CAGNNNCTG 1 cut(s) 567
CfoI GCGC 2 cut(s) 151, 218
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 4 cut(s) 302, 313, 405, 558
CseI GACGC 2 cut(s) 109, 609
CviAII CATG 3 cut(s) 274, 553, 594
DdeI CTNAG 3 cut(s) 15, 306, 617
DinI GGCGCC 1 cut(s) 150
DpnI GATC 3 cut(s) 223, 477, 494
DpnII GATC 3 cut(s) 221, 475, 492
EciI GGCGGA 2 cut(s) 192, 204
Eco130I CCWWGG 3 cut(s) 204, 225, 593
Eco24I GRGCYC 1 cut(s) 350
Eco31I GGTCTC 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 437
Eco81I CCTNAGG 1 cut(s) 306
Eco88I CYCGRG 2 cut(s) 80, 349
EcoRII CCWGG 2 cut(s) 88, 407
EcoT14I CCWWGG 3 cut(s) 204, 225, 593
EcoT22I ATGCAT 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 350
EgeI GGCGCC 1 cut(s) 150
EheI GGCGCC 1 cut(s) 150
ErhI CCWWGG 3 cut(s) 204, 225, 593
Esp3I CGTCTC 1 cut(s) 592
FaeI CATG 3 cut(s) 277, 556, 597
FaiI YATR 7 cut(s) 41, 275, 336, 398, 554, 595, 642
FalI AAGNNNNNCTT 2 cut(s) 503, 535
FatI CATG 3 cut(s) 273, 552, 593
FauI CCCGC 1 cut(s) 374
FokI GGATG 2 cut(s) 106, 311
FriOI GRGCYC 1 cut(s) 350
FspBI CTAG 2 cut(s) 516, 666
GlaI GCGC 2 cut(s) 150, 217
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 6 cut(s) 209, 304, 315, 407, 431, 560
HapII CCGG 3 cut(s) 152, 316, 432
HgaI GACGC 2 cut(s) 109, 609
HhaI GCGC 2 cut(s) 151, 218
Hin1I GRCGYC 3 cut(s) 101, 116, 149
Hin1II CATG 3 cut(s) 277, 556, 597
Hin6I GCGC 2 cut(s) 149, 216
HinP1I GCGC 2 cut(s) 149, 216
HindIII AAGCTT 1 cut(s) 574
HinfI GANTC 2 cut(s) 11, 319
HpaII CCGG 3 cut(s) 152, 316, 432
HphI GGTGA 4 cut(s) 262, 446, 485, 499
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 4 cut(s) 16, 240, 417, 480
Hpy8I GTNNAC 1 cut(s) 25
Hpy99I CGWCG 1 cut(s) 289
HpyAV CCTTC 2 cut(s) 176, 288
HpyCH4III ACNGT 2 cut(s) 292, 634
HpyCH4IV ACGT 1 cut(s) 116
HpyCH4V TGCA 1 cut(s) 338
HpyF10VI GCNNNNNNNGC 9 cut(s) 82, 103, 109, 157, 183, 215, 301, 375, 378
HpyF3I CTNAG 3 cut(s) 15, 306, 617
HpySE526I ACGT 1 cut(s) 116
Hsp92I GRCGYC 3 cut(s) 101, 116, 149
Hsp92II CATG 3 cut(s) 277, 556, 597
HspAI GCGC 2 cut(s) 149, 216
KasI GGCGCC 1 cut(s) 148
Kzo9I GATC 3 cut(s) 221, 475, 492
LmnI GCTCC 2 cut(s) 229, 345
Lsp1109I GCAGC 2 cut(s) 96, 219
LweI GCATC 2 cut(s) 84, 573
MaeI CTAG 2 cut(s) 516, 666
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 1 cut(s) 546
MalI GATC 3 cut(s) 223, 477, 494
MboI GATC 3 cut(s) 221, 475, 492
MboII GAAGA 3 cut(s) 430, 502, 514
MflI RGATCY 1 cut(s) 221
MhlI GDGCHC 2 cut(s) 350, 535
MluCI AATT 2 cut(s) 442, 657
Mly113I GGCGCC 1 cut(s) 149
MlyI GAGTC 2 cut(s) 20, 313
MmeI TCCRAC 1 cut(s) 616
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 3 cut(s) 363, 578, 670
MslI CAYNNNNRTG 1 cut(s) 553
MspA1I CMGCKG 2 cut(s) 109, 468
MspI CCGG 3 cut(s) 152, 316, 432
MspR9I CCNGG 3 cut(s) 90, 153, 409
MvaI CCWGG 2 cut(s) 90, 409
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 9 cut(s) 82, 103, 109, 157, 183, 215, 301, 375, 378
NarI GGCGCC 1 cut(s) 149
NciI CCSGG 1 cut(s) 153
NcoI CCATGG 1 cut(s) 593
NdeII GATC 3 cut(s) 221, 475, 492
NheI GCTAGC 1 cut(s) 515
NlaIII CATG 3 cut(s) 277, 556, 597
NlaIV GGNNCC 4 cut(s) 129, 150, 347, 591
NmuCI GTSAC 1 cut(s) 546
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 2 cut(s) 277, 556
OliI CACNNNNGTG 1 cut(s) 553
PciI ACATGT 1 cut(s) 552
PflFI GACNNNGTC 1 cut(s) 322
PleI GAGTC 2 cut(s) 19, 313
PluTI GGCGCC 1 cut(s) 152
PpsI GAGTC 2 cut(s) 19, 313
PscI ACATGT 1 cut(s) 552
Psp6I CCWGG 2 cut(s) 88, 407
PspGI CCWGG 2 cut(s) 88, 407
PspN4I GGNNCC 4 cut(s) 129, 150, 347, 591
PspPI GGNCC 4 cut(s) 302, 313, 405, 558
PstNI CAGNNNCTG 1 cut(s) 567
PsuI RGATCY 1 cut(s) 221
PsyI GACNNNGTC 1 cut(s) 322
RseI CAYNNNNRTG 1 cut(s) 553
SaqAI TTAA 3 cut(s) 363, 578, 670
Sau3AI GATC 3 cut(s) 221, 475, 492
Sau96I GGNCC 4 cut(s) 302, 313, 405, 558
SchI GAGTC 2 cut(s) 20, 313
ScrFI CCNGG 3 cut(s) 90, 153, 409
SduI GDGCHC 2 cut(s) 350, 535
SfaNI GCATC 2 cut(s) 84, 573
SfoI GGCGCC 1 cut(s) 150
SmiMI CAYNNNNRTG 1 cut(s) 553
SmlI CTYRAG 1 cut(s) 541
SmoI CTYRAG 1 cut(s) 541
SpeI ACTAGT 1 cut(s) 665
Sse9I AATT 2 cut(s) 442, 657
SspDI GGCGCC 1 cut(s) 148
SspMI CTAG 2 cut(s) 516, 666
StyD4I CCNGG 3 cut(s) 88, 151, 407
StyI CCWWGG 3 cut(s) 204, 225, 593
TaaI ACNGT 2 cut(s) 292, 634
TaiI ACGT 1 cut(s) 119
TaqI TCGA 1 cut(s) 447
TasI AATT 2 cut(s) 442, 657
TauI GCSGC 9 cut(s) 106, 109, 177, 189, 372, 375, 378, 381, 471
Tru1I TTAA 3 cut(s) 363, 578, 670
Tru9I TTAA 3 cut(s) 363, 578, 670
TscAI CASTG 2 cut(s) 27, 143
TseFI GTSAC 1 cut(s) 546
TseI GCWGC 2 cut(s) 109, 232
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 1 cut(s) 657
TspRI CASTG 2 cut(s) 27, 143
Tth111I GACNNNGTC 1 cut(s) 322
XceI RCATGY 2 cut(s) 277, 556
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XspI CTAG 2 cut(s) 516, 666
ZraI GACGTC 1 cut(s) 117
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.