MD01G1217700.v1.1

At4g00950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009927
Physical Location & Seq
Reverse (-)
31019484 .. 31028094
8611 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1217700.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGTGTCGGAACCAAGCATAACACCGAAGCTGTCGCTGTTCTCGCTTCCTTCCCAGCCGCCGGAGTCGCCGGGGATGCTAACTCCGCCGCTCCAAACCTCCGCCTCGGTTCCATTCCAGTGGGAGGAAGCTCCAGGAAAGCCGAGGAACTGCGGTGGCAGCGCAGAATCCAAACCCAAGTGCGCCAGATCCTTGGAGCTGCCTCCGAGGTTGCTGACGGAGGCGGCCAAAACTACCAACATGACCTCCCCGACCACCATGTTGGACGAGCCTGACGTTGGGCGGACTATGTCGTTTTCGTTCAGAGTTAGGAGCCCTGATGCTTTTGGGATCAGTAAGAGGTTGAGCAAGGAGAGTTGTAGTAGGGGCGCCAGCGGTTTTGGGTCTATGAGGTGGGGGAGTTTTAGGAAGAGCAAGGAGGTTGTTGACCATGGCGGCTTTGACTTTTTGCCTCCGGCTAGTGGCGGCGGTGGCGGTGGTGAAACGAAGGTGAATATCACAAGAGTTGGGAGGAGAGCCGGTTTCTTGAATGATTCCAACACAAGGTCACGTATGTGGGCAAGCATTTGCGAAAGCTTTAAGCAGGTGGTCCCATGGAGACGCAGGCAAGAAAAAATGAGAAAAATGGCCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF688

Protein Analysis

212

Amino Acids

23.02

Weight (kDa)

10.2

Isoelectric Point (pI)

69.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF688 PF05097 17 - 71 1e-05 Protein of unknown function (DUF688)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 574
Acc36I ACCTGC 1 cut(s) 574
AccB1I GGYRCC 1 cut(s) 368
AccBSI CCGCTC 1 cut(s) 91
AclWI GGATC 2 cut(s) 183, 338
AcoI YGGCCR 1 cut(s) 225
AcyI GRCGYC 1 cut(s) 369
AfiI CCNNNNNNNGG 5 cut(s) 61, 124, 278, 461, 543
AgsI TTSAA 1 cut(s) 529
AjnI CCWGG 1 cut(s) 133
AleI CACNNNNGTG 1 cut(s) 553
AluBI AGCT 4 cut(s) 31, 131, 199, 576
AluI AGCT 4 cut(s) 31, 131, 199, 576
Alw26I GTCTC 1 cut(s) 592
AlwI GGATC 2 cut(s) 183, 338
AoxI GGCC 2 cut(s) 225, 627
ApeKI GCWGC 2 cut(s) 159, 199
AspLEI GCGC 3 cut(s) 164, 185, 371
AspS9I GGNCC 1 cut(s) 589
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 2 cut(s) 491, 502
AvaII GGWCC 1 cut(s) 589
BanI GGYRCC 1 cut(s) 368
BanII GRGCYC 1 cut(s) 317
BbvI GCAGC 2 cut(s) 171, 186
BciT130I CCWGG 1 cut(s) 135
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 592
BfaI CTAG 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 574
BisI GCNGC 7 cut(s) 59, 89, 160, 200, 225, 436, 466
BlsI GCNGC 7 cut(s) 60, 90, 161, 201, 226, 437, 467
Bme1390I CCNGG 2 cut(s) 72, 135
Bme18I GGWCC 1 cut(s) 589
BmgT120I GGNCC 1 cut(s) 589
BmiI GGNNCC 5 cut(s) 12, 111, 314, 370, 591
BmrFI CCNGG 2 cut(s) 72, 135
BmsI GCATC 2 cut(s) 66, 310
BpmI CTGGAG 1 cut(s) 117
BpuMI CCSGG 1 cut(s) 72
BsaAI YACGTR 1 cut(s) 551
BsaHI GRCGYC 1 cut(s) 369
BsaJI CCNNGG 7 cut(s) 71, 105, 143, 192, 206, 430, 593
BsaXI ACNNNNNCTCC 4 cut(s) 230, 260, 505, 535
Bsc4I CCNNNNNNNGG 5 cut(s) 61, 124, 278, 461, 543
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 1 cut(s) 118
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 7 cut(s) 71, 105, 143, 192, 206, 430, 593
BseGI GGATG 1 cut(s) 81
BseLI CCNNNNNNNGG 5 cut(s) 61, 124, 278, 461, 543
BseNI ACTGG 1 cut(s) 118
BseRI GAGGAG 1 cut(s) 526
BseXI GCAGC 2 cut(s) 171, 186
BseYI CCCAGC 1 cut(s) 54
BshFI GGCC 2 cut(s) 227, 629
BshNI GGYRCC 1 cut(s) 368
BsiSI CCGG 4 cut(s) 62, 71, 455, 519
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 5 cut(s) 61, 124, 278, 461, 543
BsmAI GTCTC 1 cut(s) 592
BsmBI CGTCTC 1 cut(s) 592
BsmFI GGGAC 1 cut(s) 575
BsnI GGCC 2 cut(s) 227, 629
Bsp1286I GDGCHC 1 cut(s) 317
Bsp143I GATC 2 cut(s) 188, 330
Bsp19I CCATGG 2 cut(s) 430, 593
BspANI GGCC 2 cut(s) 227, 629
BspLI GGNNCC 5 cut(s) 12, 111, 314, 370, 591
BspMI ACCTGC 1 cut(s) 574
BspPI GGATC 2 cut(s) 183, 338
BspQI GCTCTTC 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 368
BsrBI CCGCTC 1 cut(s) 91
BsrFI RCCGGY 1 cut(s) 518
BsrI ACTGG 1 cut(s) 118
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 7 cut(s) 71, 105, 143, 192, 206, 430, 593
BssMI GATC 2 cut(s) 188, 330
BssNI GRCGYC 1 cut(s) 369
BssT1I CCWWGG 3 cut(s) 192, 430, 593
Bst2UI CCWGG 1 cut(s) 135
Bst6I CTCTTC 1 cut(s) 404
BstACI GRCGYC 1 cut(s) 369
BstBAI YACGTR 1 cut(s) 551
BstC8I GCNNGC 3 cut(s) 373, 562, 605
BstDSI CCRYGG 2 cut(s) 430, 593
BstF5I GGATG 1 cut(s) 81
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 3 cut(s) 164, 185, 371
BstKTI GATC 2 cut(s) 191, 333
BstMAI GTCTC 1 cut(s) 592
BstMBI GATC 2 cut(s) 188, 330
BstMWI GCNNNNNNNGC 6 cut(s) 43, 67, 76, 85, 159, 471
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 2 cut(s) 70, 133
BstV1I GCAGC 2 cut(s) 171, 186
BstX2I RGATCY 1 cut(s) 188
BstXI CCANNNNNNTGG 3 cut(s) 120, 193, 262
BstYI RGATCY 1 cut(s) 188
BsuRI GGCC 2 cut(s) 227, 629
BtgI CCRYGG 2 cut(s) 430, 593
BtsCI GGATG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 125
BveI ACCTGC 1 cut(s) 574
Cac8I GCNNGC 3 cut(s) 373, 562, 605
CfoI GCGC 3 cut(s) 164, 185, 371
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 1 cut(s) 589
CseI GACGC 1 cut(s) 609
CviAII CATG 4 cut(s) 241, 259, 431, 594
DinI GGCGCC 1 cut(s) 370
DpnI GATC 2 cut(s) 190, 332
DpnII GATC 2 cut(s) 188, 330
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 404
EarI CTCTTC 1 cut(s) 404
EciI GGCGGA 3 cut(s) 75, 91, 298
Eco130I CCWWGG 3 cut(s) 192, 430, 593
Eco24I GRGCYC 1 cut(s) 317
Eco47I GGWCC 1 cut(s) 589
EcoRII CCWGG 1 cut(s) 133
EcoT14I CCWWGG 3 cut(s) 192, 430, 593
EcoT38I GRGCYC 1 cut(s) 317
EgeI GGCGCC 1 cut(s) 370
EheI GGCGCC 1 cut(s) 370
ErhI CCWWGG 3 cut(s) 192, 430, 593
Esp3I CGTCTC 1 cut(s) 592
FaeI CATG 4 cut(s) 244, 262, 434, 597
FaiI YATR 8 cut(s) 20, 242, 260, 290, 389, 432, 554, 595
FaqI GGGAC 1 cut(s) 575
FatI CATG 4 cut(s) 240, 258, 430, 593
Fnu4HI GCNGC 7 cut(s) 59, 89, 160, 200, 225, 436, 466
FokI GGATG 1 cut(s) 88
FriOI GRGCYC 1 cut(s) 317
Fsp4HI GCNGC 7 cut(s) 59, 89, 160, 200, 225, 436, 466
FspBI CTAG 1 cut(s) 459
GlaI GCGC 3 cut(s) 163, 184, 370
GluI GCNGC 7 cut(s) 59, 89, 160, 200, 225, 436, 466
GsaI CCCAGC 1 cut(s) 58
GsuI CTGGAG 1 cut(s) 117
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 2 cut(s) 227, 629
HapII CCGG 4 cut(s) 62, 71, 455, 519
HgaI GACGC 1 cut(s) 609
HhaI GCGC 3 cut(s) 164, 185, 371
Hin1I GRCGYC 1 cut(s) 369
Hin1II CATG 4 cut(s) 244, 262, 434, 597
Hin6I GCGC 3 cut(s) 162, 183, 369
HinP1I GCGC 3 cut(s) 162, 183, 369
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 1 cut(s) 574
HinfI GANTC 3 cut(s) 65, 167, 533
HpaII CCGG 4 cut(s) 62, 71, 455, 519
HphI GGTGA 2 cut(s) 491, 502
Hpy166II GTNNAC 1 cut(s) 427
Hpy188I TCNGA 3 cut(s) 10, 207, 305
Hpy188III TCNNGA 1 cut(s) 526
Hpy8I GTNNAC 1 cut(s) 427
HpyAV CCTTC 2 cut(s) 60, 481
HpyCH4IV ACGT 2 cut(s) 276, 550
HpyF10VI GCNNNNNNNGC 6 cut(s) 43, 67, 76, 85, 159, 471
HpySE526I ACGT 2 cut(s) 276, 550
Hsp92I GRCGYC 1 cut(s) 369
Hsp92II CATG 4 cut(s) 244, 262, 434, 597
HspAI GCGC 3 cut(s) 162, 183, 369
KasI GGCGCC 1 cut(s) 368
Kzo9I GATC 2 cut(s) 188, 330
LguI GCTCTTC 1 cut(s) 404
LmnI GCTCC 4 cut(s) 96, 136, 196, 312
Lsp1109I GCAGC 2 cut(s) 171, 186
LweI GCATC 2 cut(s) 66, 310
MaeI CTAG 1 cut(s) 459
MaeII ACGT 2 cut(s) 276, 550
MaeIII GTNAC 1 cut(s) 546
MalI GATC 2 cut(s) 190, 332
MbiI CCGCTC 1 cut(s) 91
MboI GATC 2 cut(s) 188, 330
MboII GAAGA 1 cut(s) 421
MflI RGATCY 1 cut(s) 188
MhlI GDGCHC 1 cut(s) 317
Mly113I GGCGCC 1 cut(s) 369
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 2 cut(s) 243, 561
MseI TTAA 1 cut(s) 579
MslI CAYNNNNRTG 2 cut(s) 118, 553
MspA1I CMGCKG 1 cut(s) 375
MspI CCGG 4 cut(s) 62, 71, 455, 519
MspR9I CCNGG 2 cut(s) 72, 135
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 6 cut(s) 43, 67, 76, 85, 159, 471
NarI GGCGCC 1 cut(s) 369
NciI CCSGG 1 cut(s) 72
NcoI CCATGG 2 cut(s) 430, 593
NdeII GATC 2 cut(s) 188, 330
NlaIII CATG 4 cut(s) 244, 262, 434, 597
NlaIV GGNNCC 5 cut(s) 12, 111, 314, 370, 591
NmeAIII GCCGAG 1 cut(s) 168
NmuCI GTSAC 1 cut(s) 546
OliI CACNNNNGTG 1 cut(s) 553
PaqCI CACCTGC 1 cut(s) 574
PciSI GCTCTTC 1 cut(s) 404
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PfeI GAWTC 2 cut(s) 167, 533
PflFI GACNNNGTC 1 cut(s) 289
PfoI TCCNGGA 1 cut(s) 133
PkrI GCNGC 7 cut(s) 60, 90, 161, 201, 226, 437, 467
PleI GAGTC 1 cut(s) 73
PluTI GGCGCC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 73
Ppu21I YACGTR 1 cut(s) 551
Psp6I CCWGG 1 cut(s) 133
PspFI CCCAGC 1 cut(s) 54
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 5 cut(s) 12, 111, 314, 370, 591
PspPI GGNCC 1 cut(s) 589
PsuI RGATCY 1 cut(s) 188
PsyI GACNNNGTC 1 cut(s) 289
RseI CAYNNNNRTG 2 cut(s) 118, 553
SapI GCTCTTC 1 cut(s) 404
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 7 cut(s) 59, 89, 160, 200, 225, 436, 466
Sau3AI GATC 2 cut(s) 188, 330
Sau96I GGNCC 1 cut(s) 589
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 2 cut(s) 72, 135
SduI GDGCHC 1 cut(s) 317
SfaNI GCATC 2 cut(s) 66, 310
SfoI GGCGCC 1 cut(s) 370
SinI GGWCC 1 cut(s) 589
SmiMI CAYNNNNRTG 2 cut(s) 118, 553
SspDI GGCGCC 1 cut(s) 368
SspMI CTAG 1 cut(s) 459
StyD4I CCNGG 2 cut(s) 70, 133
StyI CCWWGG 3 cut(s) 192, 430, 593
TaiI ACGT 2 cut(s) 279, 553
TauI GCSGC 5 cut(s) 61, 91, 227, 438, 468
TfiI GAWTC 2 cut(s) 167, 533
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TscAI CASTG 1 cut(s) 125
TseFI GTSAC 1 cut(s) 546
TseI GCWGC 2 cut(s) 159, 199
Tsp45I GTSAC 1 cut(s) 546
TspGWI ACGGA 1 cut(s) 233
TspRI CASTG 1 cut(s) 125
Tth111I GACNNNGTC 1 cut(s) 289
VpaK11BI GGWCC 1 cut(s) 589
XspI CTAG 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.